CN201648397U - Device for applying oscillatory constant fluid shear stress action to adherent cell - Google Patents
Device for applying oscillatory constant fluid shear stress action to adherent cell Download PDFInfo
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- CN201648397U CN201648397U CN2010201419096U CN201020141909U CN201648397U CN 201648397 U CN201648397 U CN 201648397U CN 2010201419096 U CN2010201419096 U CN 2010201419096U CN 201020141909 U CN201020141909 U CN 201020141909U CN 201648397 U CN201648397 U CN 201648397U
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- fluid
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- oscillatory
- liquid
- microliter syringe
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Abstract
The utility model discloses a device for applying oscillatory constant fluid shear stress action to adherent cell, which consists of a microlitre injector, two connecting pipes, a fluid small room, an oscillatory fluid loading device and a singlechip. The microlitre injector is connected with a liquid in-out opening at one end of the fluid small room through one connecting pipe; the other connecting pipe is connected with a liquid in-out opening at the other end of the fluid small room and is inserted into a liquid storing bottle; the oscillatory fluid loading device can evenly and accurately control the injecting volume and the injecting speed of the injector when working, thereby controlling the flow speed and the oscillation frequency of the liquid in the oscillatory fluid loading device to perform reciprocating motion. The device is simple in structure and convenient in operation, can be formed into a sealing environment, can avoid bacterial contamination during experiment research, and can adjust the oscillation frequency and the size of the action shear force.
Description
Technical field
The utility model belongs to the biomechanics field, relates to a kind of cyto-mechanics deceleration loading device, specifically, relates to a kind of device that is used for the attached cell of vitro culture is applied oscillatory constant fluid shear action.
Background technology
Hydrodynamic shear is ubiquitous a kind of stress in blood flow and the osseous tissue etc. in vivo.For example for the osseous tissue of poroelastic medium, the mechanics loading can cause ground substance of bone and the deformation that is present in the liquid flow path in the bone space, causes interstitial liquid-flow, thereby produces fluid shear stress.This fluid shear stress is an oscillatory type.
Deliver in the article that is entitled as Shear stress induced stimulationof mammalian cell metabolism in 1988 people such as JA Frangos, at first proposed a kind of parallel flat stream chamber, in the cyto-mechanics experiment, be used widely.It is mainly used in adhesive attraction between research cell and cell, cell and the base material etc., and the while also is used to study the reaction of bone and its cells to mechanical stimulation.On the basis of this research, it is that 200420034438.3 patent of invention has openly been described a kind of stream of cells mechanics experimental installation that the patent No. is also arranged.This stream of cells mechanics experimental installation is by down liquid storage tank, peristaltic pump, last liquid storage tank and parallel flat stream chamber four most of compositions, between the each several part by pipe connection.Place slide glass on the groove of the chute central authorities of its parallel flat stream chamber, the groove periphery of chute is provided with sealing groove, put silica gel sealing ring in the sealing groove, be equipped with on cover plate, the cover plate on the parallel flat stream chamber and install cushion plate, the two ends of stream chamber are fixed by the screw on the metal fixture B, and the opening at place, stream gangway, chamber is connected conducting with rubber hose with last liquid storage tank respectively with following liquid storage tank.The front and back of parallel flat stream chamber are fixed by the screw on the metal fixture A, and metal fixture A is provided with vision slit, and four metal fixtures are looped around fixing formation parallel flat stream chamber integral body around the stream chamber.This parallel flat stream chamber design has mainly solved the easy pollution problem of cell in afterburner process.
This stream of cells mechanics experimental installation provides power by peristaltic pump, but pair cell carries out the processing research of one-way fluid shearing force.Its weak point is, though the structure of parallel flat stream chamber solves the easy pollution problem after the reinforcing, what its structure was unfavorable for testing carries out fast.
The utility model provides a kind of experimental installation that can load oscillatory constant fluid shearing force, the effect of the oscillating fluid shearing force in the analog bone tissue well, and the effect of oscillating fluid in other tissue, and then can study of the influence of two-way hydrodynamic shear to attached cell.And the fluid cell is not only constructed simple relatively, and also vulnerable to pollution not after the reinforcing.
The utility model content
The technical problems to be solved in the utility model provides the device that a kind of attached cell to vitro culture applies oscillatory constant fluid shear action, study of the effect of different oscillatory constant fluid shearing forces, can also study of the influence of two-way hydrodynamic shear attached cell to attached cell.Characteristics of the present utility model are that apparatus structure is simple, easy to operate and can form an airtight environment, can prevent the pollution of bacterium and can regulate oscillation frequency and the size of effect shearing force in experimental study.
Above-mentioned technical problem of the present utility model is by providing a kind of following structure that has, attached cell applied the device of oscillatory constant fluid shear action solves, this device comprises microliter syringe, pipe connecting, fluid cell, silica gel sealing ring, oscillatory fluid deceleration loading device and micro-chip.
Described microliter syringe is to be connected by the liquid entrance of pipe connecting with fluid cell one end; Another root pipe connecting connects the liquid entrance of the other end of fluid cell, and inserts in the liquid storage bottle.Microliter syringe is fixed in the installation microliter syringe group draw-in groove in the oscillatory fluid deceleration loading device, promptly constitutes the integral body of whole device.
Described oscillatory fluid deceleration loading device is by the translational motion group of motors, and the linear reciprocating motion coaster is installed microliter syringe group draw-in groove compositions such as (seven).
The stepper-motor rotating screw bolt promotes nut, makes foot rest make linear uniform motion.Motor forward or reverse, then corresponding foot rest are done to advance or setback.
The coaster that foot rest drive straight line moves is done at the uniform velocity straight reciprocating motion.
The microliter syringe pipe shaft is installed on the workplatform, maintains static.Microliter syringe piston rod tail end is fixed on the coaster with jig, and is being affected by coaster and to do at the uniform velocity straight reciprocating motion, thereby makes flowing that liquid in the microliter syringe does to release or draw back.What this fluidic was reciprocal flows, and is transferred in the fluid cell by pipe connecting.
The motor uniform rotation makes coaster produce linear uniform motion.The coaster movement velocity reaches reciprocating cycle and amplitude by micro-chip control, and is adjustable continuously.
Place sheet glass on the chute of fluid cell central authorities, sheet glass is loaded with one of cell and faces down; The chute periphery is provided with sealing groove, places silica gel sealing ring on the sealing groove; The fluid cell is provided with cover plate, and carries out screwing hermetic by 8 symmetrical screws on every side.
The utility model applies the device of oscillatory constant fluid shear action to attached cell, and is simple in structure, easy handling; And because its volume is little, whole device comprises the syringe pusher, can be positioned in the cell culture incubator, so just can guarantee the constant of pH that the fluid cell is interior and temperature fully.
The utility model is simple to the device aseptically process that attached cell applies oscillatory constant fluid shear action.Pipe connecting, screw can be handled with autoclaving, and fluid cell part, syringe can clean with pure water earlier, immerse 75% ethanol again and cross liquid.Be positioned over air-dry getting final product under the ultra-clean ultraviolet lamp before the experiment, guaranteed aseptic after the reinforcing.
The utility model device adopts oscillatory fluid deceleration loading device that motivating force is provided, and can evenly, accurately control the volume injected and the injection speed of microliter syringe, thereby flow velocity and the oscillation frequency of controlling liquid in it is carried out reciprocating.By oscillatory fluid deceleration loading device, can regulate and control the size of hydrodynamic shear continuously.
The utility model is the pipe connecting that adopts polytetrafluoroethylmaterial material to make to the device that attached cell applies oscillatory constant fluid shear action, its duct wall good rigidity, under fluid pressure action, be difficult for to produce significant deformation, can guarantee that therefore the power that cell is suffered is basic identical with the power of oscillatory fluid deceleration loading device driving.
Description of drawings
Below in conjunction with accompanying drawing the utility model is described in further detail the device that attached cell applies oscillatory constant fluid shear action.
Fig. 1 applies the device synoptic diagram of oscillatory constant fluid shear action to attached cell for the utility model.
Fig. 2 is the oscillatory fluid deceleration loading device front view of the utility model device.
Fig. 3 is the oscillatory fluid deceleration loading device sectional view of the utility model device.
Fig. 4 is the fluid cell front view of the utility model device.
Fig. 5 is the fluid cell sectional view of the utility model device.
1 micro-chip, 2 oscillatory fluid deceleration loading devices, 3 microliter syringes, 4 pipe connectings
5 fluid cells 6 are installed draw-in groove 7 stepper-motors 8 jigs 9 coasters
10 foot rests, 11 screw holes, 12 liquid entrances, 13 sealing grooves
14 fluid cell inner chamber liquid in-out mouths, 15 central chutes
Embodiment
, be the utility model applies oscillatory constant fluid shear action to attached cell device at first referring to Fig. 1.This device is made up of microliter syringe 3, pipe connecting 4, fluid cell 5, silica gel sealing ring, oscillatory fluid deceleration loading device 2 and micro-chip 1.Described microliter syringe 3 is to be connected by the liquid entrance 12 of pipe connecting 4 with fluid cell 5 one ends; Another root pipe connecting 4 connects the liquid entrance 12 of the other end of fluid cell 5, and inserts in the liquid storage bottle.Microliter syringe 3 is fixed in the installation microliter syringe group draw-in groove 6 in the oscillatory fluid deceleration loading device 2, promptly constitutes the integral body of whole device.The to-and-fro movement of microliter syringe 3 plungers promptly forms the oscillatory hydrodynamic shear effect that attached cell is applied.
Fig. 2 and Fig. 3 have showed the oscillatory fluid deceleration loading device of the utility model device.Described oscillatory fluid deceleration loading device is by the translational motion stepper-motor, and the linear reciprocating motion coaster is installed microliter syringe group draw-in groove compositions such as (seven).Its stepper-motor 7 rotating screw bolts promote nut, make foot rest 10 make linear uniform motion.Stepper-motor 7 forward or reverse, then corresponding foot rest 10 is done to advance or setback.The coaster 9 that foot rest 10 drive straight lines move is done straight reciprocating motion.It is motionless that microliter syringe 3 pipe shafts are installed in syringe draw-in groove 6 internal fixing.Microliter syringe 3 piston rod tail ends are fixed on the coaster 9 with jig 8, and affected by coaster 9 reciprocating, thereby make flowing that liquid in the microliter syringe 3 do to release or draw back.Stepper-motor 7 uniform rotation make coaster 9 produce linear uniform motion.Coaster 9 movement velocitys and reciprocating cycle and amplitude realize that by rotating speed, the rotating commutation cycle of micro-chip 1 control step motor 7 cycle and amplitude are adjustable continuously.
Fig. 4 and Fig. 5 are the synoptic diagram of the fluid cell of the utility model device.Central chute 15 parts of fluid cell 5 are placed sheet glass, and sheet glass is loaded with one of cell and faces down; The chute periphery is provided with sealing groove 13, places silica gel sealing rings in the sealing groove 13, and fluid cell 5 tops are provided with cover plate, cover plate and fluid cell 5 fixedly be to screw and seal by its 8 symmetrical screws on every side.5 liquid in-out mouths 12 by taper of fluid cell are fastenedly connected pipe 4, and pipe connecting 4 also is connected with microliter syringe 3.
The utility model applies the device of oscillatory constant fluid shear action to attached cell, its pipe connecting 4 adopts polytetrafluoroethylmaterial material to make, pipe connecting 4 duct wall good rigidity, be difficult for embrittlement, under fluid pressure action, be difficult for to produce significant deformation, can guarantee that therefore the power that cell is suffered is basic identical with the power of oscillatory fluid deceleration loading device driving.
The material of described fluid cell 5 is adopted as polymethylmethacrylate.
The utility model is simple to the device aseptically process that attached cell applies oscillatory constant fluid shear action.Pipe connecting 4, screw can be handled with autoclaving, fluid cell 5 parts, microliter syringe 3 must be cleaned with pure water earlier, immerse 75% ethanol again and cross liquid, are positioned over air-dry getting final product under the ultra-clean ultraviolet lamp before the experiment, have guaranteed aseptic after the reinforcing.Whole device comprises the syringe pusher, can put into cell culture incubator, can guarantee the constant of pH in the fluid cell and temperature fully.
Claims (5)
1. device that attached cell is applied oscillatory constant fluid shear action, form by microliter syringe, pipe connecting, fluid cell, oscillatory fluid deceleration loading device and micro-chip, it is characterized in that: microliter syringe (3) links to each other with the liquid entrance (12) of fluid cell (5) one ends by pipe connecting (4), another root pipe connecting (4) connects the liquid entrance (12) of fluid cell (5) the other end, and in the insertion liquid storage bottle, pipe connecting (4) fixes by the liquid in-out mouth (12) of fluid cell (5) taper.
2. the device that attached cell is applied oscillatory constant fluid shear action according to claim 1, it is characterized in that: central chute (15) part of fluid cell (5) is placed sheet glass, the chute periphery is provided with sealing groove (13), the built-in silica gel sealing ring of sealing groove (13), fluid cell (5) top is provided with cover plate, and cover plate and fluid cell (5) are by 8 fastening and sealings of symmetrical screw.
3. the device that attached cell is applied oscillatory constant fluid shear action according to claim 1, it is characterized in that: during oscillatory fluid deceleration loading device (2) work, its stepper-motor (7) rotating screw bolt promotes nut, make foot rest (10) drive coaster (9) and do at the uniform velocity straight reciprocating motion, stepper-motor (7) uniform rotation makes coaster (9) produce linear uniform motion, coaster (9) movement velocity and reciprocating cycle and amplitude are by micro-chip (1) control, and cycle and amplitude are adjustable continuously.
4. the device that attached cell is applied oscillatory constant fluid shear action according to claim 1, it is characterized in that: microliter syringe (3) pipe shaft is fixed in the installation microliter syringe group draw-in groove (6) in the oscillatory fluid deceleration loading device (2), microliter syringe piston rod tail end is fixed on the coaster (9) with jig (8), and affected reciprocating by coaster (9), make flowing that liquid in the microliter syringe does to release or draw back, the to-and-fro movement of microliter syringe (3) plunger promptly forms the oscillatory hydrodynamic shear effect that attached cell is applied.
5. according to claim 1 attached cell is applied the device of oscillatory constant fluid shear action, it is characterized in that: the material of pipe connecting (4) is a tetrafluoroethylene, and the material of fluid cell (5) is a polymethylmethacrylate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201419096U CN201648397U (en) | 2010-03-25 | 2010-03-25 | Device for applying oscillatory constant fluid shear stress action to adherent cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201419096U CN201648397U (en) | 2010-03-25 | 2010-03-25 | Device for applying oscillatory constant fluid shear stress action to adherent cell |
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| CN201648397U true CN201648397U (en) | 2010-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010201419096U Expired - Fee Related CN201648397U (en) | 2010-03-25 | 2010-03-25 | Device for applying oscillatory constant fluid shear stress action to adherent cell |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206574A (en) * | 2011-04-27 | 2011-10-05 | 中国人民解放军第四军医大学 | Automatic cutting device used for primary culture of cells and lysis of tissues |
| CN103409312A (en) * | 2013-07-18 | 2013-11-27 | 新疆医科大学 | Cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion |
| CN104007238A (en) * | 2014-06-10 | 2014-08-27 | 北京航空航天大学 | Film surface uniform shearing force loading device |
-
2010
- 2010-03-25 CN CN2010201419096U patent/CN201648397U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206574A (en) * | 2011-04-27 | 2011-10-05 | 中国人民解放军第四军医大学 | Automatic cutting device used for primary culture of cells and lysis of tissues |
| CN103409312A (en) * | 2013-07-18 | 2013-11-27 | 新疆医科大学 | Cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion |
| CN103409312B (en) * | 2013-07-18 | 2014-12-10 | 新疆医科大学 | Cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion |
| CN104007238A (en) * | 2014-06-10 | 2014-08-27 | 北京航空航天大学 | Film surface uniform shearing force loading device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101124 Termination date: 20130325 |