CN102206574A - Automatic cutting device used for primary culture of cells and lysis of tissues - Google Patents
Automatic cutting device used for primary culture of cells and lysis of tissues Download PDFInfo
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- CN102206574A CN102206574A CN2011101074776A CN201110107477A CN102206574A CN 102206574 A CN102206574 A CN 102206574A CN 2011101074776 A CN2011101074776 A CN 2011101074776A CN 201110107477 A CN201110107477 A CN 201110107477A CN 102206574 A CN102206574 A CN 102206574A
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- sleeve pipe
- knife
- mounting plate
- shaped body
- cylinder
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Abstract
The invention discloses an automatic cutting device used for primary culture of cells and lysis of tissues. The device comprises a cylindrical main body and a pair of scissors above the cylindrical main body, wherein a mounting plate is fixed inside the cylindrical main body, a miniature DC (direct current) motor is located below the mounting plate, the motor shaft of the DC motor passes through the mounting plate and is fixedly connected with a turntable, a pushing rod is connected with a rail in a vertical and sliding mode through a sliding chute below the pushing rod, and one end of a crank is connected with the turntable through a shaft and the other end is connected with one end of the pushing rod through a shaft; the scissors comprise a movable handle and a stationary handle and the movable handle is located inside a movable handle casing; and the stationary handle is located inside a stationary handle casing, the stationary handle casing is positioned by a fixing component of the upper end cover of the cylindrical main body, the end of the stationary handle casing extends to the mounting plate close to the turntable, the movable handle casing passes through the avoidance hole on the upper end cover of the cylindrical main body and enters the cylindrical main body, the end of the movable handle casing extends to the inside of the other end of the pushing rod, and a restoring spring is arranged between the stationary handle casing and the movable handle casing on the fixing component.
Description
Technical field
The present invention relates to a kind of in medical teaching and scientific experiment cell former be commissioned to train foster, organize the automatic shear of cracking
Background technology
In recent years, along with the development that deepens continuously of cytology research, cell cultures also becomes one of the most frequently used means of biology and medical research, it be divided into former be commissioned to train to support and go down to posterity cultivate two kinds.Formerly be commissioned to train that to support be directly to obtain cell from organism to cultivate, because cell just separates from biological tissue, so more approach biological intravital animation.Former growth, metabolism, the breeding of supporting to postgraduate's object cell of being commissioned to train provides strong means, also cultivates for going down to posterity later on simultaneously to create conditions.Utilize aforesaid method also can directly serve clinical practice.For example, with from the operation the excision tumour cell carry out former be commissioned to train foster, carry out the screening of cancer therapy drug then with this culturing cell, the susceptibility of the chemotherapeutics of adding is helped select the most effective chemotherapy regimen, might play the effect that heightens the effect of a treatment, reduces side effect according to tumour cell.
Former be commissioned to train to support mainly contain two kinds of tissue block method and digestion methods.Wherein digestion method mainly is to obtain cell by the enzymolysis tissue.The first step of above-mentioned two kinds of methods all needs tissue block is separated, and shreds.Though former generation, culture method was a kind of simple and easy to do and cultural method that success ratio is higher, most tissues piece isolation technique all is manual operations at present, directly influences tissue block size degree of uniformity, and complex steps, length consuming time not only increases pollution probability, has a strong impact on cell survival rate simultaneously.
Summary of the invention
The present invention is directed to the tissue block hand and operate existing problem, a kind of science be provided, saved time, made things convenient for, efficient and can improve cell survival rate organize the cracking shear, resource, time can significantly be saved, reduce the crossed contamination in the experimentation, and can overcome in the past this drawback tissue block cell injury that excessive shear causes.
For reaching above purpose, the present invention takes following technical scheme to be achieved:
A kind of cell is former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that, the scissors that comprises a cylinder-shaped body and cylinder-shaped body top, wherein the cylinder-shaped body internal fixing has mounting plate, the mounting plate below is provided with a DC micromotor, the mounting plate top is provided with a rotating disk, level to track, a push rod and a crank, the motor shaft of direct-current machine passes mounting plate and rotating disk is connected, the chute of push rod below it is vertical with track to be slidingly connected, crank one end is by axle connection rotating disk, and the crank the other end connects an end of push rod by axle;
Scissors comprises the movable handle of a knife and the static handle of a knife of the static cutter head of cutting out section and movable cutter head, power section, and the movable blade sleeve is in movable blade sleeve pipe; Static handle of a knife is enclosed within the static handle of a knife sleeve pipe, static handle of a knife sleeve pipe is by the mounting block location of cylinder-shaped body upper end cover, its end extends near the mounting plate of rotating disk, the hole of dodging that movable blade sleeve pipe passes the cylinder-shaped body upper end cover enters cylinder-shaped body, its end extends to the inboard of the push rod the other end, is provided with return spring between the static handle of a knife sleeve pipe on mounting block top and movable blade sleeve pipe.
In the such scheme, described return spring is upper and lower respectively to be provided with an arc track, and an end of this arc track and static handle of a knife sleeve pipe connect firmly, and the roller that is provided with on the other end and the movable blade sleeve pipe is slidingly matched, during shearing motion, movable blade sleeve pipe is made arc and is slided in arc track.
Described static handle of a knife tube ends is provided with the circumference of elastic plectrum towards rotating disk.
The blade arranged outside of described static cutter head end is scraping blade in a organized way, the vertical perk of its front end, and the whole scraping blade of organizing is L-shaped.
Ratio between the arm of force of described scissor cut part and power section is 1: 3.Described movable blade sleeve pipe and static handle of a knife telescopic angle are 30 °.
Described direct-current machine is realized rotating speed control by being arranged on the lateral speed control switch of cylinder-shaped body.
Adopt the advantage of apparatus of the present invention to be: simplify the tissue block separating step, weak point consuming time, the isolating size of tissue block is even, reduces pollution probability, significantly improves the former foster survival rate of being commissioned to train of cell.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is that the C of Fig. 1 is to view.
Fig. 3 is the I portion enlarged view among Fig. 1.
Fig. 4 is the tissue container synoptic diagram of band ice chest.
Fig. 5 is the vertical view of tissue container among Fig. 4.
Among Fig. 1 to Fig. 5: 1, movable cutter head; 2, static handle of a knife; 3, return spring; 4, static handle of a knife sleeve pipe; 5, mounting block; 6, elastic plectrum; 7, rotating disk; 8, mounting plate; 9, projection; 10, dodge the hole; 10 ', dodge the hole; 11, crank; 12, axle; 13, track; 14, push rod; 15, movable blade sleeve pipe; 16, movable handle of a knife; 17, static cutter head; 18, static handle of a knife tube ends; 19, motor shaft; 20, push rod inboard; 21, chute; 22, organize scraping blade; 23, sleeve; 24, ice chest; 25, bar is proposed; 26, screen cloth; 27, arc track.
Embodiment
The present invention is described in further detail below in conjunction with drawings and the specific embodiments.
Shown in Figure 2 as Fig. 1, a kind of cell is former be commissioned to train foster, organize the automatic shear of cracking, the scissors that comprises a cylinder-shaped body and cylinder-shaped body top, wherein the cylinder-shaped body internal fixing has mounting plate 8, the mounting plate below is provided with a DC micromotor, this direct-current machine can be realized rotating speed control (not drawing among the figure) by being arranged on the lateral speed control switch of cylinder-shaped body, the mounting plate top is provided with a rotating disk 7, a horizontal rail 13, a push rod 14 and a crank 11, the motor shaft 19 of direct-current machine passes mounting plate 8 and is connected with rotating disk 7, the chute 21 of push rod 14 below it and track 13 vertical being slidingly connected, crank 11 1 ends connect rotating disk 7 by axle 12, and the crank the other end connects an end of push rod 14 by axle 12.
Scissors comprises the movable handle of a knife 16 and the static handle of a knife 2 of the static cutter head 17 of cutting out section and movable cutter head 1, power section, and movable handle of a knife 16 is enclosed within the movable blade sleeve pipe 15; Static handle of a knife 2 is enclosed within the static handle of a knife sleeve pipe 4, and handle of a knife and casing-pipe connection structure are adopted in power section, and its purpose is Convenient scissors installation, dismounting (cleaning) and meets the principle of aseptic technique.Static handle of a knife sleeve pipe 4 is by mounting block 5 location of cylinder-shaped body upper end cover, and its end extends near the rotating disk 7 the mounting plate, and this static handle of a knife tube ends 18 is provided with the circumference of elastic plectrum 6 towards rotating disk 7; The hole 10 ' of dodging that movable blade sleeve pipe 15 passes the cylinder-shaped body upper end cover enters cylinder-shaped body, and its end extends to the inboard 20 of push rod 14 the other ends.Between the static handle of a knife sleeve pipe 4 on mounting block 5 tops and movable blade sleeve pipe 15, be provided with return spring 3.
The return spring 3 of scissors butt joint is upper and lower respectively to be provided with an arc track 27, one end of this arc track and static handle of a knife sleeve pipe 4 connect firmly, the roller that is provided with on the other end and the movable blade sleeve pipe 15 is slidingly matched, during as shearing motion, movable blade sleeve pipe is made arc and is slided in arc track 27, purpose is to reduce vibration scissors is caused uncertain factor on the direction, strengthens the stability of scissors, improves the efficient of scissor cut.
The principle of work of above shear is: motor shaft 19 drives rotating disk 7 and turns clockwise, change push rod 14 into along track 13 relative rotating disk moving linearlies by crank 11 and axle 12 circumferential motion with rotating disk, push rod 14 moves as arc to static handle of a knife sleeve pipe 4 ends 18 away from promoting movable blade sleeve pipes 15 ends with the other end of crank coupling end inboard 20, make movable handle of a knife 16 make shearing motion in opposite directions with static handle of a knife 2, because having strip on mounting plate 8 dodges hole 10, therefore can not hinder the arcuate movement of movable blade sleeve pipe 15 ends.When rotating disk 7 rotation half cycles, the static cutter head 17 and the movable cutter head 2 of scissors close up; When rotating disk 7 rotates a circle, under the effect of return spring 3, be returned to initial state again, a shear action of scissors is finished, and goes round and begins again like this, can realize shearing function automatically.
Be arranged with projection 9 on the circumference of rotating disk 7, can stir elastic plectrum when this projection rotates to elastic plectrum 6 along with rotating disk and make static handle of a knife sleeve pipe 4 vibrations, to remove the tissue debris of institute's adhesive tape on the cutter head.
As shown in Figure 3, the blade arranged outside of static cutter head 17 ends is scraping blade 21 in a organized way, and the vertical perk of its front end is L-shaped.The purpose of organizing scraping blade 21 is set is the dispersive tissue of concentrating in the tissue container and be beneficial to shear, and in filtration procedure, play the conduction shock effect and be convenient to organize abundant filtration, finally obtain the former foster required big or small tissue block of being commissioned to train.
Ratio between the arm of force of scissor cut part and power section is 1: 3, and movable blade sleeve pipe 15 is 30 ° with the angle of static handle of a knife sleeve pipe 4, and its advantage is the shear surface size to fit that can make the scissor cut part, improves shear efficiency.
As Fig. 4, shown in Figure 5, when the automatic shear of the present invention uses, the cutting out section of scissors can be put in the tissue container, replace operating by hand originally scissors.The tissue container inner bottom part is provided with an annulus platform, with the convenient tissue of shearing; Annulus platform central authorities are recessed a sleeve 23, can inlay the different screen cloth of order number 26 below the sleeve 23, is used to filter the tissue block of collecting required different sizes.Annulus platform, sleeve 23, screen cloth 26 can be made of one, and put or take out tissue container into proposing bar 25.Tissue container can be placed on operation in the supporting ice chest 24, and to keep cell number and activity, ice chest is done by rubber, and rubber is divided into inside and outside two-layer, adds refrigerating fulid in intermediate course.
Claims (7)
- A cell former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that, the scissors that comprises a cylinder-shaped body and cylinder-shaped body top, wherein the cylinder-shaped body internal fixing has mounting plate, the mounting plate below is provided with a DC micromotor, the mounting plate top is provided with a rotating disk, level to track, a push rod and a crank, the motor shaft of direct-current machine passes mounting plate and rotating disk is connected, the chute of push rod below it is vertical with track to be slidingly connected, crank one end is by axle connection rotating disk, and the crank the other end connects an end of push rod by axle;Scissors comprises the movable handle of a knife and the static handle of a knife of the static cutter head of cutting out section and movable cutter head, power section, and the movable blade sleeve is in movable blade sleeve pipe; Static handle of a knife is enclosed within the static handle of a knife sleeve pipe, static handle of a knife sleeve pipe is by the mounting block location of cylinder-shaped body upper end cover, its end extends near the mounting plate of rotating disk, the hole of dodging that movable blade sleeve pipe passes the cylinder-shaped body upper end cover enters cylinder-shaped body, its end extends to the inboard of the push rod the other end, is provided with return spring between the static handle of a knife sleeve pipe on mounting block top and movable blade sleeve pipe.
- 2. cell as claimed in claim 1 is former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that, described return spring is upper and lower respectively to be provided with an arc track, one end of this arc track and static handle of a knife sleeve pipe connect firmly, the roller that is provided with on the other end and the movable blade sleeve pipe is slidingly matched, during shearing motion, movable blade sleeve pipe is made arc and is slided in arc track.
- 3. cell as claimed in claim 1 is former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that described static handle of a knife tube ends is provided with the circumference of elastic plectrum towards rotating disk.
- 4. cell as claimed in claim 1 is former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that the blade arranged outside of static cutter head end is scraping blade in a organized way, the vertical perk of its front end, the whole scraping blade of organizing is L-shaped.
- 5. cell cultures as claimed in claim 1, organize the automatic shear of cracking, it is characterized in that, the ratio between the arm of force of scissor cut part and power section is 1: 3.
- As claim 1 or 5 described cells former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that movable blade sleeve pipe and static handle of a knife telescopic angle are 30 °.
- 7. cell as claimed in claim 1 is former be commissioned to train foster, organize the automatic shear of cracking, it is characterized in that described direct-current machine is realized rotating speed control by being arranged on the lateral speed control switch of cylinder-shaped body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110107477 CN102206574B (en) | 2011-04-27 | 2011-04-27 | Automatic cutting device used for primary culture of cells and lysis of tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110107477 CN102206574B (en) | 2011-04-27 | 2011-04-27 | Automatic cutting device used for primary culture of cells and lysis of tissues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102206574A true CN102206574A (en) | 2011-10-05 |
| CN102206574B CN102206574B (en) | 2013-01-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110107477 Expired - Fee Related CN102206574B (en) | 2011-04-27 | 2011-04-27 | Automatic cutting device used for primary culture of cells and lysis of tissues |
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| Country | Link |
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| CN (1) | CN102206574B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486043A (en) * | 2018-02-02 | 2018-09-04 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
| CN109846531A (en) * | 2019-02-26 | 2019-06-07 | 河南亚都实业有限公司 | Hysteromyomectomy negative pressure catheter |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2571766Y (en) * | 2002-08-23 | 2003-09-10 | 中国科学院生物物理研究所 | Liquid self-circulation cell shearing raising pool |
| CN1524948A (en) * | 2003-02-25 | 2004-09-01 | 清华大学 | Apparatus for supersonic cracking cell and cutting macro molecule and method for its use |
| CN100339469C (en) * | 2002-11-19 | 2007-09-26 | 韩国科学技术研究院 | Hybrid bioreactor for cell culture |
| CN101469307A (en) * | 2007-12-28 | 2009-07-01 | 财团法人工业技术研究院 | Cloning apparatus, and segmentation and acquisition mechanism thereof |
| CN201425554Y (en) * | 2009-04-30 | 2010-03-17 | 夏淑君 | Scissors for shearing cells |
| CN201648397U (en) * | 2010-03-25 | 2010-11-24 | 西北工业大学 | Device for applying oscillatory constant fluid shear stress action to adherent cell |
-
2011
- 2011-04-27 CN CN 201110107477 patent/CN102206574B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2571766Y (en) * | 2002-08-23 | 2003-09-10 | 中国科学院生物物理研究所 | Liquid self-circulation cell shearing raising pool |
| CN100339469C (en) * | 2002-11-19 | 2007-09-26 | 韩国科学技术研究院 | Hybrid bioreactor for cell culture |
| CN1524948A (en) * | 2003-02-25 | 2004-09-01 | 清华大学 | Apparatus for supersonic cracking cell and cutting macro molecule and method for its use |
| CN101469307A (en) * | 2007-12-28 | 2009-07-01 | 财团法人工业技术研究院 | Cloning apparatus, and segmentation and acquisition mechanism thereof |
| CN201425554Y (en) * | 2009-04-30 | 2010-03-17 | 夏淑君 | Scissors for shearing cells |
| CN201648397U (en) * | 2010-03-25 | 2010-11-24 | 西北工业大学 | Device for applying oscillatory constant fluid shear stress action to adherent cell |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486043A (en) * | 2018-02-02 | 2018-09-04 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
| CN108486043B (en) * | 2018-02-02 | 2019-03-19 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
| CN109846531A (en) * | 2019-02-26 | 2019-06-07 | 河南亚都实业有限公司 | Hysteromyomectomy negative pressure catheter |
| CN109846531B (en) * | 2019-02-26 | 2023-11-21 | 河南亚都实业有限公司 | Suction catheter for hysteromyectomy |
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| Publication number | Publication date |
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| CN102206574B (en) | 2013-01-09 |
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Granted publication date: 20130109 Termination date: 20140427 |