CN202830046U - In-vitro culture device for mammalian embryos - Google Patents
In-vitro culture device for mammalian embryos Download PDFInfo
- Publication number
- CN202830046U CN202830046U CN 201220554755 CN201220554755U CN202830046U CN 202830046 U CN202830046 U CN 202830046U CN 201220554755 CN201220554755 CN 201220554755 CN 201220554755 U CN201220554755 U CN 201220554755U CN 202830046 U CN202830046 U CN 202830046U
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- flow path
- path channel
- embryo
- culture device
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model relates to an in-vitro culture device for mammalian embryos. The culture device is formed by binding a lower flow path channel layer and an upper main body layer, wherein a flow path channel is arranged in the flow path channel layer; an electromagnetic coil is arranged under the middle part of the flow path channel, and connected with a square wave generator through a lead; a membrane is arranged on the main body layer; culture chambers are arranged surround the membrane, and communicated with each other through the flow path channel; a magnetic piece is arranged on the membrane and located above the electromagnetic coil; and a line switch is arranged on the lead. In the use of the in-vitro culture device, an embryo culture solution and mineral oil are added to the culture chamber at first; the membrane is indirectly driven by the square wave generator to move so that the embryo culture solution and the mineral oil move; growth and development of an embryo in the body of a mammal under the condition of the microscopic environment is simulated; and the embryo is stimulated so as to grow in dynamic, microscopic and three-dimensional environment; and the developmental rate of blastula is increased and the number of cells of the cell mass of the blastula is increased.
Description
Technical field
The utility model relates to animal embryo inventionculture tools field, what be specifically related to is a kind of mammal embryo vitro culture device.
Background technology
The In vitro culture technology is one of the most basic gordian technique of embryo IVC in the embryo engineering.Traditional In vitro culture device mainly is culture dish or culture plate, this class culture apparatus only can for the embryo provide a kind of static state, the macroscopic view, the two dimension the growth in vitro environment, with the embryo in vivo growing environment differ greatly.Therefore; use this class culture apparatus usually can change embryo's the correlation properties such as growth velocity, form, the activity of born of the same parents' intracellular metabolite; cause embryo in vitro fertilization when follow-up use culture dish or culture plate carry out vitro culture; the developmental rate of blastaea is lower; take mouse as example; the developmental rate of blastaea only has 30%～40%, and the cell count of inner cell mass does not reach yet cultivates the number that requires.
In addition, the embryo between the growth period, also can be subject to the extruding of Oviductal Smooth Muscles in vivo, the effect of the power such as laminar shear of friction and Oviductal Fluid; Simultaneously, in addition the nutritive substance that the embryo not only needs nutrient solution to provide in growth, growth course, also needs the stimulation of certain physical property mechanical force, promotes it to grow.And being traditional In vitro culture ware or culture plate, the stimulation of these physical property mechanical forces can't directly provide.
The utility model content
Goal of the invention of the present utility model is to provide a kind of mammal embryo incubator for the problems referred to above, be used for solving embryo in vitro fertilization when follow-up use culture dish or culture plate carry out vitro culture, because there is the problem that blastocyst rate is low, the cell count of inner cell mass is few in the stimulation that the physical property mechanical force can't directly be provided.
The utility model is achieved through the following technical solutions: a kind of mammal embryo vitro culture device is by the flow path channel layer that is positioned at the below and the body layer above being positioned at is bonded, wherein, be provided with flow path channel in the flow path channel layer, below, flow path channel middle part is provided with solenoid, and solenoid is connected with square-wave generator by wire; Be provided with diaphragm on the body layer, be provided with culturing room around the diaphragm, be communicated with by flow path channel between the culturing room; Be provided with magnetic sheet on the diaphragm, magnetic sheet is positioned at the top of solenoid; Be provided with line switch on the described wire.
Described flow path channel layer below is stained with sheet glass.
Adopt the positively effect of technique scheme:
When 1, the utility model uses, at first in culturing room, add embryo medium, mineral oil, indirectly drive motion of membrane by square-wave generator, thereby make embryo medium, mineral oil motion, simulation the embryo grow under the microscopy environment condition in mammalian body, stimulate the embryo, make that the embryo is in dynamically, growing environment microcosmic, three-dimensional, improved the cell count of blastocyst rate and increase blastaea inner cell mass;
2, the utility model is simple to operate, easy to use, is fit to apply.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Among the figure: 1 flow path channel layer, 2 body layers, 3 flow path channels, 4 solenoids, 5 wires, 6 square-wave generators, 7 line switchs, 8 diaphragms, 9 culturing room, 10 magnetic sheets, 11 sheet glass.
Embodiment
In order to be illustrated more clearly in the technical solution of the utility model, below in conjunction with concrete diagram, further set forth content of the present utility model.
As shown in Figure 1, a kind of mammal embryo vitro culture device is to be bonded by the flow path channel layer 1 that is positioned at the below and the body layer 2 that is positioned at the top.Wherein, be provided with flow path channel 3 in the flow path channel layer 1, and below flow path channel 3 middle parts solenoid 4 be set, solenoid 4 is connected with square-wave generator 6 by wire 5, is provided with line switch 7 on the wire 5.Be provided with diaphragm 8 on the body layer 2, be provided with culturing room 9 around the diaphragm 8, be communicated with by flow path channel 3 between the culturing room 9; Be provided with magnetic sheet 10 on the diaphragm 8, magnetic sheet 10 is positioned at the top of solenoid 4.After connecting square-wave generator 6, magnetic sheet 10 can move by band dynamic diaphragm 8 together, diaphragm 8 motions will drive the liquid movement in culturing room 9 and the flow path channel 3, thereby reach the purpose of fetal development in the analogue body, stimulate embryo growth, improve blastocyst rate, increased the cell count of blastaea inner cell mass.
Flow path channel layer 1, body layer 2 and diaphragm 8 be when producing in the utility model, can select that biocompatibility is strong, transparency is high, the avirulent polydimethylsiloxane material of cell is prepared.Simultaneously, in order further to improve whole firm degree, also can add sheet glass 11 below flow path channel layer 1, sheet glass 11 is bonded as one with flow path channel layer 1.
Magnetic sheet 10 can be selected the magnetic sheet of neodymium iron boron material in the utility model.
During use, the requirement condition of at first growing according to different mammal embryos is set the frequency of square-wave generator 6; The nutrient solution that fetal development is required, mineral oil inject flow path channel 3 from culturing room 9 again, and embryo in vitro fertilization is put into culturing room, simultaneously this incubator are placed microscopically, confirm that the embryo has placed culturing room; Connect line switch 7; Whole incubator is put in the incubator that contains 5% carbonic acid gas cultivates, and carry out the length of setup times according to the mammiferous fostering requirement of difference.Like this, realization vitro culture work that just can be simple, convenient, efficient.
Claims (2)
1. mammal embryo vitro culture device, it is characterized in that: this incubator is by the flow path channel layer that is positioned at the below and the body layer above being positioned at is bonded, wherein, be provided with flow path channel (3) in the flow path channel layer, below, flow path channel (3) middle part is provided with solenoid (4), and solenoid (4) is connected with square-wave generator (6) by wire (5); Be provided with diaphragm (8) on the body layer (2), diaphragm (8) is provided with culturing room (9) on every side, is communicated with by flow path channel (3) between the culturing room (9); Be provided with magnetic sheet (10) on the diaphragm (8), magnetic sheet (10) is positioned at the top of solenoid (4); Be provided with line switch (7) on the described wire (5).
2. a kind of mammal embryo vitro culture device according to claim 1 is characterized in that: be stained with sheet glass (11) below the described flow path channel layer (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220554755 CN202830046U (en) | 2012-10-26 | 2012-10-26 | In-vitro culture device for mammalian embryos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220554755 CN202830046U (en) | 2012-10-26 | 2012-10-26 | In-vitro culture device for mammalian embryos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202830046U true CN202830046U (en) | 2013-03-27 |
Family
ID=47941800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220554755 Withdrawn - After Issue CN202830046U (en) | 2012-10-26 | 2012-10-26 | In-vitro culture device for mammalian embryos |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202830046U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876573A (en) * | 2012-10-26 | 2013-01-16 | 黑龙江八一农垦大学 | Mammal embryo in-vitro culture device and culture method thereof |
-
2012
- 2012-10-26 CN CN 201220554755 patent/CN202830046U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876573A (en) * | 2012-10-26 | 2013-01-16 | 黑龙江八一农垦大学 | Mammal embryo in-vitro culture device and culture method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20130327 Effective date of abandoning: 20131120 |
|
| RGAV | Abandon patent right to avoid regrant |