CN204981904U - Pipeline formula cell culture device - Google Patents
Pipeline formula cell culture device Download PDFInfo
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- CN204981904U CN204981904U CN201520493763.4U CN201520493763U CN204981904U CN 204981904 U CN204981904 U CN 204981904U CN 201520493763 U CN201520493763 U CN 201520493763U CN 204981904 U CN204981904 U CN 204981904U
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- cell culture
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Abstract
The utility model discloses a pipeline formula cell culture device, include through the cell culture module and the nourishment cyclic delivery module of pipeline intercommunication, be equipped with the cultivation pipeline that is the S -shaped and coils the setting in the cell culture module. The adherence space of the cell that cultivation pipeline that the S -shaped coiled the setting increases makes the cell can grow in circuitous pipeline, great improvement reproduction space and the utilization ratio of material of cell.
Description
Technical field
The utility model relates to a kind of cell culture apparatus, particularly relates to a kind of duct type cell culture apparatus.
Background technology
In animal cell culture process, very high to the requirement of environment, need to prevent microbiological contamination, when usually adopting culturing bottle culturing cell, directly prepare nutrient solution to inoculate cell, until after cell proliferation to some amount and then express need product or access virus.Because the cell culturing space of culturing bottle is limited, the utilization ratio of culturing bottle is not high.
Utility model content
For above deficiency, the utility model provides a kind of duct type cell culture apparatus, and it is single use device, and its make and easy to use, with low cost, utilization ratio is high and health rank is high.
To achieve these goals, the technical solution adopted in the utility model is: a kind of duct type cell culture apparatus, comprise media to cell culture modules, the cultivation pipeline that S-shaped coiling is arranged is provided with in described media to cell culture modules, the two side of media to cell culture modules is respectively equipped with inlet opening and fluid hole, described inlet opening and fluid hole are communicated with the two ends of cultivating pipeline respectively; Also comprise nutriment circulation conveyor module, described nutriment circulation conveyor module is communicated with fluid hole with the inlet opening of media to cell culture modules respectively by pipeline, and the pipeline between connection nutriment circulation conveyor module and inlet opening is provided with peristaltic pump.
Preferably, described media to cell culture modules inside is the chamber of hollow, the chamber top of this hollow, and the inner roof wall position being positioned at media to cell culture modules is provided with several pieces vertically to downward-extension and spaced diaphragm; The chamber lower portion of hollow, the diapire position being positioned at media to cell culture modules is provided with several pieces and vertically upwards extends and spaced diaphragm, to misplace to each other grafting to the diaphragm of downward-extension and the diaphragm that upwards extends, to make the cultivation pipeline forming the coiling of S shape between diaphragm.
Preferably, described diaphragm is other PE diaphragm of medical grade, and all diaphragms all adopt the mode of welding to be fixed in the chamber of the hollow of media to cell culture modules.
Preferably, described media to cell culture modules is set at least one.
Preferably, the inlet opening of described media to cell culture modules and fluid hole are respectively equipped with fast joint.
Preferably, described nutriment circulation conveyor module comprises tank body and control module, control module is arranged in the nutrient solution of tank body, described control module comprises whipping appts, breather, temperature-control device, potential of hydrogen control device and dissolved oxygen control device, for stir culture liquid, to pass into gas and control the temperature of nutrient solution, potential of hydrogen and dissolved oxygen to nutrient solution.
The beneficial effects of the utility model:
1) diaphragm of dislocation grafting is set in cell cultures film block, make between diaphragm, to define flat roundabout S shape and cultivate pipeline, thus the adherent space of the cell increased, cell is enable to grow in roundabout pipeline, improve the breeding space of cell greatly, just can breed more cell, improve the utilization ratio of cell cultures film block.
2) cell cultures film block is set to disposable pre-sterilized module, makes user not need before use to carry out complicated sterilising treatment, open bag and use, reduce time cost.
3) cell cultures film block is combined by fast joint and pipeline and nutriment circulation conveyor module, therefore the magnitude setting of cell cultures film block can not be limit, make multiple cell cultures film block can carry out the object of cell cultures simultaneously, substantially increase the efficiency of cell cultures work.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
In figure, 1, nutriment circulation conveyor module, 11, tank body, 12, control module, 2, media to cell culture modules, 21, cultivate pipeline, 22, diaphragm, 23, inlet opening, 25, fluid hole, 3, peristaltic pump.
Embodiment
Be further elaborated the utility model below in conjunction with accompanying drawing, wherein, direction of the present utility model take Fig. 1 as standard.
With reference to Fig. 1, duct type cell culture apparatus of the present utility model, comprise nutriment circulation conveyor module 1 and media to cell culture modules 2, media to cell culture modules 2 is linked together by pipeline and nutriment circulation conveyor module 1, is provided the existence supply of cell in media to cell culture modules 2 by nutriment circulation conveyor module 1.
Nutriment circulation conveyor module 1 comprises tank body 11 and control module 12, and tank body 11 adopts glass to make, for storing nutrient solution.
Control module 12 comprises whipping appts, breather, temperature-control device, potential of hydrogen control device and dissolved oxygen control device, control module 12 is put into the nutrient solution of tank body 11, for stir culture liquid, pass into gas to nutrient solution, the temperature of nutrient solution, potential of hydrogen and dissolved oxygen are controlled.
Media to cell culture modules 2 entirety is in square, its inside is the chamber of hollow, the roundabout cultivation pipeline 21 for making cell attachment is provided with in the chamber of this hollow, make the S-shaped coiling of cultivation pipeline 21, the top of the chamber of hollow, the inner roof wall being positioned at media to cell culture modules 2 is provided with several pieces parallel and spaced diaphragms, and diaphragm 22 is other PE diaphragm of medical grade, all diaphragms 22 are all vertically extended downwards
The bottom of the chamber of hollow, the diapire being positioned at media to cell culture modules 2 is provided with several pieces parallel and spaced diaphragms 22, and all diaphragms 22 are all vertically upwards extended,
To misplace to each other grafting to the diaphragm 22 of downward-extension and the diaphragm 22 that upwards extends, thus make between diaphragm 22, to form the flat roundabout cultivation pipeline 21 circulated for nutrient solution, all diaphragms 22 all adopt the mode of welding to be fixed in the chamber of the hollow of media to cell culture modules 2
The two side of media to cell culture modules 2 is respectively equipped with inlet opening 23 and fluid hole 24, inlet opening 23 and fluid hole 24 are communicated with the two ends of cultivating pipeline 21 respectively, and inlet opening 23 and fluid hole 24 are installed with the disposable fast joint of sanitation-grade respectively.
Inlet opening 23 is connected with pipeline by fast joint respectively with fluid hole 24, pipeline then inserts in the nutrient solution of nutriment circulation conveyor module 1 tank body 11 respectively, which form the path for nutrient solution circulation, pipeline between connection nutriment circulation conveyor module 1 and inlet opening 23 is provided with peristaltic pump 3, thus forces nutrient solution to be circulated between nutriment circulation conveyor module 1 and media to cell culture modules 2 by pipeline.
Media to cell culture modules 2 and fast joint are disposable accessory, before using, media to cell culture modules 2 connects the disposable fast joint of sanitation-grade, then sealed and packed rear irradiation sterilization can be packed, meanwhile, just because of media to cell culture modules 2 is disposable pre-sterilized module, therefore user no longer needs complicated sterilising treatment before use, open bag and use, reduce time cost.
Working process of the present utility model is: during use, started nutrient solution from tank body 11 toward cultivation pipeline 21 internal recycle from peristaltic pump 3 after cultivation pipeline 21 inside is adjacent to until cell after cultivation pipeline 21 inner static for some time of single scattered zooblast aseptic access media to cell culture modules 2, flow velocity is controlled by the rotating speed of peristaltic pump 3, cultivate the potential of hydrogen of liquid, temperature, dissolved oxygen is controlled by control module 12.
When the supplementary nutrient solution of needs, by the mode of perfusion the material of cellular metabolism diluted from tank body 11 and cement out.
According to the needs of practical situation, multiple media to cell culture modules 2 culturing cell simultaneously can be set, utilize fast joint, pipeline to be combined with nutriment circulation conveyor module 1 by multiple media to cell culture modules 2, for media to cell culture modules 2 provides nutrient solution, substantially increase the efficiency of cell cultures work.
When needing the cell cultivated to take out from media to cell culture modules 2, after only need adding trysinization in cultivation pipeline 21, concussion media to cell culture modules 2, this like cell will come off, and then allows cell flow out from cultivation pipeline 21.
The foregoing is only better embodiment of the present invention, the present invention is not limited to above-mentioned embodiment, the structural modification that local is small may be there is in implementation process, if do not depart from the spirit and scope of the present invention to various change of the present invention or modification, and belonging within claim of the present invention and equivalent technologies scope, then the present invention is also intended to comprise these change and modification.
Claims (6)
1. a duct type cell culture apparatus, it is characterized in that, comprise media to cell culture modules (2), the cultivation pipeline (21) that S-shaped coiling is arranged is provided with in described media to cell culture modules (2), the two side of media to cell culture modules (2) is respectively equipped with inlet opening (23) and fluid hole (24), described inlet opening (23) and fluid hole (24) are communicated with the two ends of cultivating pipeline (21) respectively; Also comprise nutriment circulation conveyor module (1), described nutriment circulation conveyor module (1) is communicated with fluid hole (24) with the inlet opening (23) of media to cell culture modules (2) respectively by pipeline, and the pipeline between connection nutriment circulation conveyor module (1) and inlet opening (23) is provided with peristaltic pump (3).
2. duct type cell culture apparatus according to claim 1, it is characterized in that, described media to cell culture modules (2) inside is the chamber of hollow, the chamber top of this hollow, the inner roof wall position being positioned at media to cell culture modules (2) is provided with several pieces vertically to downward-extension and spaced diaphragm (22); The chamber lower portion of hollow, the diapire position being positioned at media to cell culture modules (2) is provided with several pieces and vertically upwards extends and spaced diaphragm (22), to misplace to each other grafting to the diaphragm (22) of downward-extension and the diaphragm (22) that upwards extends, to make the cultivation pipeline (21) forming the coiling of S shape between diaphragm (22).
3. duct type cell culture apparatus according to claim 2, it is characterized in that, described diaphragm (22) all adopts the mode of welding to be fixed in the chamber of the hollow of media to cell culture modules (2) for other PE diaphragm of medical grade, all diaphragms (22).
4. duct type cell culture apparatus according to claim 1, is characterized in that, described media to cell culture modules (2) is set at least one.
5. duct type cell culture apparatus according to claim 1, is characterized in that, the inlet opening (23) of described media to cell culture modules (2) and fluid hole (24) are respectively equipped with fast joint.
6. the duct type cell culture apparatus described arbitrarily according to claim 1-5, it is characterized in that, described nutriment circulation conveyor module (1) comprises tank body (11) and control module (12), control module (12) is arranged in the nutrient solution of tank body (11), described control module (12) comprises whipping appts, breather, temperature-control device, potential of hydrogen control device and dissolved oxygen control device, for stir culture liquid, to pass into gas and control the temperature of nutrient solution, potential of hydrogen and dissolved oxygen to nutrient solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520493763.4U CN204981904U (en) | 2015-07-09 | 2015-07-09 | Pipeline formula cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520493763.4U CN204981904U (en) | 2015-07-09 | 2015-07-09 | Pipeline formula cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204981904U true CN204981904U (en) | 2016-01-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520493763.4U Active CN204981904U (en) | 2015-07-09 | 2015-07-09 | Pipeline formula cell culture device |
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| Country | Link |
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| CN (1) | CN204981904U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282015A (en) * | 2016-09-27 | 2017-01-04 | 惠倪 | Systemic circulation fluid bed bioreactor and the method cultivating zooblast |
| WO2022142638A1 (en) * | 2020-12-30 | 2022-07-07 | 惠倪 | Large-circulating cell retention apparatus, fluid circulation pump, and down-detection shaker |
-
2015
- 2015-07-09 CN CN201520493763.4U patent/CN204981904U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282015A (en) * | 2016-09-27 | 2017-01-04 | 惠倪 | Systemic circulation fluid bed bioreactor and the method cultivating zooblast |
| CN106282015B (en) * | 2016-09-27 | 2019-01-04 | 惠倪 | Systemic circulation fluidized bed bioreactor and the method for cultivating zooblast |
| WO2022142638A1 (en) * | 2020-12-30 | 2022-07-07 | 惠倪 | Large-circulating cell retention apparatus, fluid circulation pump, and down-detection shaker |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Pipeline formula cell culture device Effective date of registration: 20171221 Granted publication date: 20160120 Pledgee: Guangzhou Caold financing Company limited by guarantee Pledgor: Guangzhou Qizhi Biology Engineering Equipment Co., Ltd. Registration number: 2017440000196 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20181116 Granted publication date: 20160120 Pledgee: Guangzhou Caold financing Company limited by guarantee Pledgor: Guangzhou Qizhi Biology Engineering Equipment Co., Ltd. Registration number: 2017440000196 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Pipeline formula cell culture device Effective date of registration: 20181212 Granted publication date: 20160120 Pledgee: Guangzhou Caold financing Company limited by guarantee Pledgor: Guangzhou Qizhi Biology Engineering Equipment Co., Ltd. Registration number: 2018440000363 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220321 Granted publication date: 20160120 Pledgee: Guangzhou Caold financing Company limited by guarantee Pledgor: GUANGZHOU QIZHI BIOLOGICAL ENGINEERING EQUIPMENT CO.,LTD. Registration number: 2018440000363 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |