CN201261787Y - Cell cultivation apparatus for exerting mechanical stimulation on cell - Google Patents
Cell cultivation apparatus for exerting mechanical stimulation on cell Download PDFInfo
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- CN201261787Y CN201261787Y CN 200820108541 CN200820108541U CN201261787Y CN 201261787 Y CN201261787 Y CN 201261787Y CN 200820108541 CN200820108541 CN 200820108541 CN 200820108541 U CN200820108541 U CN 200820108541U CN 201261787 Y CN201261787 Y CN 201261787Y
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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Abstract
The utility model discloses a cell culture device for exerting mechanical stimulation on cells, which comprises a cupped culture dish provided with a cover on the upper end surface; a small hole is formed on the bottom surface of the cupped culture dish; a vacuum chamber is positioned at the lower end of the cupped culture dish; a transparent cover glass is arranged at the lower end of the vacuum chamber; biocompatibility elastic membrane is mounted on the upper end surface of the small hole or the top surface of the vacuum chamber corresponding to the small hole; a vacuum extractor is connected with the vacuum chamber and can vacuumize the vacuum chamber; and lubricant is coated on the upper surface of the transparent cover glass. When evacuation is performed on the vacuum chamber by the vacuum extractor, the elastic membrane can be pressed towards and clung on the elastic membrane in the vacuum chamber and is stretched in a two dimensional surface of the upper surface of the cover glass. Horizontal dynamic change of molecules can be observed in real time through a microscope arranged under the vacuum chamber, various types of mechanical stimulation can be produced by controlling the shapes and sizes of the elastic membrane and the cross section of the vacuum chamber, and the complicated mechanical environment in corpora can be better simulated.
Description
Technical field:
The utility model belongs to the cell culture apparatus that is used for biomedical sector, be particularly related to the cell culture apparatus that a kind of pair cell applies mechanical stimulation, this cell culture apparatus is a kind of cell culture apparatus that is provided with elastica, adherent growth monolayer cell or stick the three-dimensional cell culture block on elastica, flexible this elastica can stretch or compress monolayer cell or three-dimensional cell culture block thereon.
Background technology
Along with the development of cytobiology, the influence of cell micro-environment cellular function more and more comes into one's own, and particularly the physiological function of mechanical stimulation pair cell plays an important role.For the effect at external better simulation mechanical stimulation, scientists study has designed many mechanical loading units.Thomas D.Brown (Journal of Biomechanics 33,3-14,2000) has summarized cell in vitro mechanics loading technique more all sidedly.Specifically, patent US5348879-A (Application of biaxialmechanical force to living cell culture-uses displacement actuatorto contact and stretch membrane on which cell culture is mounted, 1994) invented a kind of device that can apply the twin shaft mechanical stimulation to the cell of cultivation on elastica, be fixed around the elastica in this device, place a platform below the elastica, this platform is boosted under power wheel drive and is made elastica be stretched.Patent CN 101092595A (experimental apparatus for loading cell through digital controlled mechanical strain, 2007) invented a kind of to being grown in the device that cell on the elastica applies mechanical stimulation, a mechanical top board is arranged below the elastica, produce stretching action by mechanical top board roof pressure elastica.The shortcoming of these two kinds of methods be elastica in drawing process along with platform or top board move up and down, very difficult pair cell carries out Real Time Observation.
Patent US6107081-A (Unidirectional cell stretching device forstudying mechanical loading in human tissues comprises tissue culturevessel, actuator assembly and elastic strip respectively connectedto ram and fixed structure, 2000) invented a kind of device that can apply the single shaft mechanical stimulation to the cell of cultivation on elastica, bar shaped elastica two ends are clamped in this device, one end is fixed on the fixed shelf, the other end is fixed on the active shelf, the active shelf moves back and forth at power wheel drive lower edge single shaft, thereby along uniaxial extension or elasticity of compression film, the weak point of this device is that the anchor clamps of fixedly elastica are upwards raised elastica, make cell zoom out, be difficult to carry out high power lens and observe from the object lens distance of inverted microscope.Patent CN 1932511A (sinusoidal tensile cell loader, 2007) invented a kind of to being grown in the device that cell on the elastica applies the single shaft mechanical stimulation, bar shaped elastica two ends are clamped in this device, one end is fixed on the fixed shelf, the other end is fixed on the active shelf, the active shelf moves back and forth at power wheel drive lower edge single shaft, thereby along uniaxial extension or elasticity of compression film, different in this patent among the anchor clamps of elastica and the patent US6107081-A, can be so that the elastica position be downward, but and between the object lens of inverted microscope still every frame, culture dish, one section that reaches between culture dish top and the elastica is the space of substratum, can not satisfy high power lens and observe.
(the Annals of BiomedicalEngineering such as Mohammad Sotoudeh of Shu Chien study group, Vol.26, pp.181189,1998) research and design a kind of mechanically operated mechanical loading unit, can stimulate axle stretchings such as elastica apply, its elastica is placed on the polytetrafluoroethylene ring on the stationary platform, fix with a ring device around the elastica, the annular stationary installation is connected with a mechanical driver unit, the control mechanical driver unit moves up and down and can stretch or elasticity of compression film, because polytetrafluoroethylene ring is a fixed, elastica is only in same planar motion in telescopic process.Patent US4839280-A (Apparatus for applying stress to cell cultures, 1989), US4789601-A (Polysiloxane compsn.surface modified to improvebiocompatibility-by incorporation of carbon, amine or carboxylicacid, esp.useful as cell culturesubstrates, 1988) and US6048723-A (Flexible bottom culture plate for applying mechanical load to cellcultures, 2000) invented the mechanical loading unit that a kind of negative pressure drives, be a kind of be the culture dish of bottom surface with the elastica, cell attachment is grown in the elastica upper surface, culture dish bottom surface (elastica) and the cavity under it have formed annular seal space, apply negative pressure and can make elastica decline produce stretching, thereby the cell on it is applied mechanical stimulation.The shortcoming of this scheme is that elastica moves up and down in the deformation process, does not remain on same plane, is unfavorable for microscopic examination, and elastica various piece deformation heterogeneity.Patent US6472202-B1 (Loading station assembly and method for tissueengineering, 2002) further develop on this basis, place a platform in the annular seal space under culture dish bottom surface (elastica), the platform upper surface contacts with the elastica lower surface, elastica above the platform is stretched on this platform upper surface when applying negative pressure like this, the shape that changes the platform upper surface can wait axle or uniaxial extension to elastica, and the difform platform of conversion can apply the various mechanical stimulations that wait axle or single shaft different directions to same sample.Patent CN 1846606A (compound mechanical stress cell loading device for simulated joint cavity, 2006) having invented can be to being grown in the device that cell on the elastica applies multiple mechanical stimulation, be fixed on the mechanical platform around its elastica, by mechanical drive tensile elasticity film.These based on common shortcomings of the mechanics loading technique of elastica are exactly: the surface of cell growth is a higher platform to be arranged below the elastica upper surface, or (US6107081-A) raised by stationary installation on every side in plane, elastica place, thereby make cell far away, the dynamic change of the molecular level of viable cell when can't use high power lens (as the oily mirror of inverted microscope etc.) Real Time Observation mechanical stimulation from the object lens of inverted microscope distance.With the common experimental technique of these devices is to apply fixing or peptic cell behind for some time mechanical stimulation, carries out conventional biochemical analysis then.This mode is suitable for studying cell and is subjected to secular variation behind the mechanical stimulation, but variation for the cell short-term, as the dynamic polar contribution of signaling molecule calcium ion, can carry out the viable cell real-time dynamic monitoring at molecular level with regard to needs, these present methods are difficult to satisfy this demand.
Mechanical loading unit all is the stretching of carrying out single type generally at present, waits axle or uniaxial extension, yet real mechanical environment is complicated and diversified in the body, and this just requires to design the mechanical loading unit of controlled complicated type.Though patent US6472202-B1 (Loading station assembly andmethod for tissue engineering, 2002) claim that platform under can the conversion elastica is to change stretching mode, but this will take whole sealing device apart, operation possibility is not strong, move in circles such as the stretching mode of two types of needs and to hocket, this scheme then is difficult to realize.
The elastica of these devices needs complicated additional components to fix at present, and complex structure is during production costs.
Summary of the invention
The purpose of this utility model is at the above weak point of existing mechanical loading unit, proposes the cell culture apparatus that a kind of pair cell applies mechanical stimulation.The molecular level dynamic change of real-time monitored viable cell when the utility model device can be implemented in pair cell and applies mechanical stimulation; By the planeform and the size of set elastica and vacuum chamber, can obtain waiting the stretch mode of axle stretching, uniaxial extension or complicated type, for the variation of research cell under complicated mechanical environment provides strong instrument.
The technical solution of the utility model is as follows:
The pair cell that the utility model provides applies the cell culture apparatus of mechanical stimulation, comprising: the cup-shaped culture dish 2 of culture dish lid 1 is stamped in a upper surface; One is positioned at the vacuum chamber 9 of described cup-shaped culture dish 3 lower ends; One vacuum extractor 8 that links to each other with described vacuum chamber 9 and described vacuum chamber 9 is vacuumized; It is characterized in that:
The bottom surface of described cup-shaped culture dish 2 is provided with aperture;
The lower surface of described vacuum chamber 9 is transparency cover slides 5;
The upper surface of described aperture or vacuum chamber 9 end faces corresponding with described aperture are equipped with biocompatible flexible film 4.
Clamp a transition medium layer 3 that has an aperture between described cup-shaped culture dish 2 and the described vacuum chamber 9, the aperture on the described transition medium layer 3 is corresponding with the aperture on described cup-shaped culture dish 2 bottom surfaces; Aperture on the described transition medium layer 3 is equal to, or greater than the aperture on described cup-shaped culture dish 2 bottom surfaces.
Pair cell of the present utility model applies the cell culture apparatus of mechanical stimulation, can comprise further that also one is arranged on the microscope of described vacuum chamber 9 belows in order to the Real Time Observation cell.
On described cup-shaped culture dish 2 bottom surfaces on the quantity of aperture and the described transition medium layer 3 quantity of aperture equate that its quantity is at least 1.
Aperture on described cup-shaped culture dish 2 bottom surfaces and the aperture on the described transition medium layer 3 are circular port, slotted eye, square hole, slot or polygonal hole.
Chemistry or physical modification are carried out in the subregion of described elastica 4 upper surfaces or upper surface.
The sidewall 6 of described vacuum chamber 9 is silica gel sidewall, side wall glass, plastic sidewalls or the metal sidewall that has no side effect.
The upper surface of described transparency cover slide 5 scribbles the lubricant that has no side effect.
The cross section of described vacuum chamber 9 is circle, ellipse, square, rectangle, I shape or Polygons.
The cross-sectional area of described vacuum chamber 9 is greater than the cross-sectional area sum of aperture on described cup-shaped culture dish 2 bottom surfaces.
The cross-sectional area of described vacuum chamber 9 is greater than the cross-sectional area sum of aperture on the described transition medium layer 3.
Described vacuum extractor 8 vacuumizes by 7 pairs of described vacuum chambers 9 of breather line; The port of described breather line 7 stretch into length in the described vacuum chamber 9 less than the sidewall of outermost aperture on described cup-shaped culture dish 2 bottom surfaces apart from the distance between the sidewall 6 of described vacuum chamber 9.
The cell culture apparatus that described pair cell applies mechanical stimulation is a plurality of, the cell culture apparatus that a plurality of pair cells apply mechanical stimulation composes in parallel a machinery, a described vacuum extractor 8 links to each other with all vacuum chambers 9 of described machinery simultaneously, and all vacuum chambers 9 are vacuumized.
Advantage of the present utility model is:
1, the utility model device is when pair cell applies mechanical stimulation, molecular level dynamic change that can the Real Time Observation viable cell, the containing much information of acquisition.And present mechanical loading unit based on elastica is difficult to realize this function, and after they were can only be by mechanical stimulation a certain amount of, fixing or peptic cell carried out biochemical analysis then, and the quantity of information of acquisition is few.
2, the utility model device can be in same device integrated controlled broad variety mechanical stimulation, for the variation of research cell under complicated mechanical environment provides strong instrument.Though patent US6472202-B1 (Loading station assembly and method for tissueengineering, 2002) claim that platform under can the conversion elastica is to change stretching mode, but this will take whole sealing device apart, operation possibility is not strong, move in circles such as the stretching mode of two types of needs and to hocket, this scheme then is difficult to realize, then is easy to realize this function with device of the present utility model.
3, the elastica of using in the utility model is made simple, does not need extra stationary installation, and good reproducibility.The elastica of present mechanical loading unit needs in addition preparation separately, and needs complicated additional components to fix, and complex structure is made loaded down with trivial details time-consumingly, and the fixed effect is bad a little promptly can to influence experimental result greatly.
4, the utility model device can be integrated, a plurality of independently mechanical loading units can link together by breather line and form a machinery, by the unified control of a common negative pressure device (vacuum extractor), so just can drive the identical or different mechanics loading unit of a plurality of types simultaneously by a negative pressure device.
Description of drawings
Fig. 1 is the structural representation (before stretching) of the utility model device (embodiment).
Fig. 2 is the structural representation (back stretches) of the utility model device.
Fig. 3 is the structural representation of the utility model device (another embodiment).
Embodiment
Further describe the utility model below in conjunction with drawings and Examples.
Fig. 1 is the structural representation (before stretching) of the utility model device; Fig. 2 is the structural representation (back stretches) of the utility model device.Fig. 3 is the structural representation of the utility model device; As seen from the figure, the pair cell that the utility model provides applies the cell culture apparatus of mechanical stimulation, comprising: the cup-shaped culture dish 2 of culture dish lid 1 is stamped in a upper surface; One is positioned at the vacuum chamber 9 of described cup-shaped culture dish 3 lower ends; One vacuum extractor 8 that links to each other with described vacuum chamber 9 and described vacuum chamber 9 is vacuumized; It is characterized in that:
The bottom surface of described cup-shaped culture dish 2 is provided with aperture;
The lower surface of described vacuum chamber 9 is transparency cover slides 5;
The upper surface of described aperture or vacuum chamber 9 end faces corresponding with described aperture are equipped with biocompatible flexible film 4.
Clamp a transition medium layer 3 that has an aperture between described cup-shaped culture dish 2 and the described vacuum chamber 9, the aperture on the described transition medium layer 3 is corresponding with the aperture on described cup-shaped culture dish 2 bottom surfaces; Aperture on the described transition medium layer 3 is equal to, or greater than the aperture on described cup-shaped culture dish 2 bottom surfaces.
Pair cell of the present utility model applies the cell culture apparatus of mechanical stimulation, can comprise further that also one is arranged on the microscope (not shown) of described vacuum chamber 9 belows in order to the Real Time Observation cell.
On described cup-shaped culture dish 2 bottom surfaces on the quantity of aperture and the described transition medium layer 3 quantity of aperture equate that its quantity is at least 1.
Aperture on described cup-shaped culture dish 2 bottom surfaces and the aperture on the described transition medium layer 3 are circular port, slotted eye, square hole, slot or polygonal hole.
Chemistry or physical modification are carried out in the subregion of described elastica 4 upper surfaces or upper surface.
The sidewall 6 of described vacuum chamber 9 is silica gel sidewall, side wall glass, plastic sidewalls or the metal sidewall that has no side effect.
The upper surface of described transparency cover slide 5 scribbles the lubricant that has no side effect.
The cross section of described vacuum chamber 9 is circle, ellipse, square, rectangle, I shape or Polygons.
The cross-sectional area of described vacuum chamber 9 is greater than the cross-sectional area sum of aperture on described cup-shaped culture dish 2 bottom surfaces.
The cross-sectional area of described vacuum chamber 9 is greater than the cross-sectional area sum of aperture on the described transition medium layer 3.
Described vacuum extractor 8 vacuumizes by 7 pairs of described vacuum chambers 9 of breather line; The port of described breather line 7 stretch into length in the described vacuum chamber 9 less than the sidewall of outermost aperture on described cup-shaped culture dish 2 bottom surfaces apart from the distance between the sidewall 6 of described vacuum chamber 9.
The cell culture apparatus that described pair cell applies mechanical stimulation is a plurality of, the cell culture apparatus that a plurality of pair cells apply mechanical stimulation composes in parallel a machinery, a described vacuum extractor 8 links to each other with all vacuum chambers 9 of described machinery simultaneously, and all vacuum chambers 9 are vacuumized.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, and as shown in Figure 1, its structure comprises:
The cup-shaped culture dish 2 of culture dish lid 1 is stamped in one upper surface, and the bottom surface of described cup-shaped culture dish 2 is provided with an aperture;
One is positioned at the vacuum chamber 9 of described cup-shaped culture dish 2 lower ends, and the lower surface of described vacuum chamber 9 is transparency cover slides 5;
Biocompatible flexible film 4 (as pellosil) is equipped with in the lower surface of described aperture;
One links to each other with described vacuum chamber 9, and breather line 7 and vacuum extractor 8 that described vacuum chamber 9 is vacuumized.
Comprise in the present embodiment that one is arranged on the microscope (not shown) of described vacuum chamber 9 belows in order to the Real Time Observation cell.
Aperture on the bottom surface of described cup-shaped culture dish 2 is a circular port, also can be slotted eye;
But described elastica 4 upper surface adherent growth monolayer cells or stick the three-dimensional cell culture block.(not shown);
The sidewall 6 of described vacuum chamber 9 is the silica gel sidewall, also can be plastic sidewalls;
The upper surface of described transparency cover slide 5 scribbles the lubricant that has no side effect (such as silicone oil); The cross section of described vacuum chamber 9 is a square, also can be rectangle, and its length of side is greater than the diameter of the aperture on described cup-shaped culture dish 2 bottom surfaces;
Described vacuum extractor 8 vacuumizes by 7 pairs of described vacuum chambers 9 of breather line, and elastica 4 is pressed towards transparency cover slide 5, and at stretch along axles such as quilts in the two dimensional surface on cover glass 5 upper surfaces (Fig. 2).
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, reach the maximum tension degree when described elastica 4 is stretched to opening in vacuum chamber of the contact mouth of pipe of described breather line 7 or described breather line 7, described vacuum extractor 8 continues described vacuum chamber 9 vacuumized can not make described elastica 4 continue to be stretched (Fig. 2).
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, and can be to stop after taking out once, keep vacuum chamber in certain negative pressure state, and described elastica 4 keeps stretched state; Also can be to vacuumize discontinuously or clocklike and recover normal pressure, described elastica 4 be stretched with certain frequency and amplitude.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, and as shown in Figure 3, its structure comprises:
The cup-shaped culture dish 2 of culture dish lid 1 is stamped in one upper surface, and the bottom surface of described cup-shaped culture dish 2 is provided with an aperture;
One is positioned at the vacuum chamber 9 of described cup-shaped culture dish 2 lower ends, and the lower surface of described vacuum chamber 9 is transparency cover slides 5;
Clamp a transition medium layer 3 that has an aperture between described cup-shaped culture dish 2 and the described vacuum chamber 9, the aperture on the described transition medium layer 3 is corresponding with the aperture on described cup-shaped culture dish 2 bottom surfaces; Aperture on the described transition medium layer 3 is equal to, or greater than the aperture on described cup-shaped culture dish 2 bottom surfaces; Biocompatible flexible film 4 (as pellosil) is equipped with in the lower surface of the aperture on the described transition medium layer 3;
One links to each other with described vacuum chamber 9, and breather line 7 and vacuum extractor 8 that described vacuum chamber 9 is vacuumized.
Comprise in the present embodiment that one is arranged on the microscope (not shown) of described vacuum chamber 9 belows in order to the Real Time Observation cell;
Aperture on the bottom surface of described cup-shaped culture dish 2 is a square hole, also can be circular port, slotted eye, slot or polygonal hole;
Aperture on the described transition medium layer 3 is a circular port, also can be slotted eye, square hole, slot or polygonal hole.
But in the subregion chemically modified of described elastica 4 upper surfaces be terminal silane molecule with the polyoxyethylene glycol, be modified with polyoxyethylene glycol and resist protein or cell adhesion for the zone of terminal silane molecule, protein or cell can only adhere to does not have the zone of chemically modified polyoxyethylene glycol for terminal silane molecule;
The sidewall 6 of described vacuum chamber 9 is a side wall glass, also can be the metal sidewall that has no side effect;
The upper surface of described transparency cover slide 5 scribbles the lubricant that has no side effect (such as edible oil); The cross section of described vacuum chamber 9 is circular, also can be ellipse or Polygons, and its area is greater than the area of the aperture on the described transition medium layer 3;
Described vacuum extractor 8 vacuumizes by 7 pairs of described vacuum chambers 9 of breather line, and elastica 4 is pressed towards transparency cover slide 5, and is stretching along axles such as quilts in the two dimensional surface on cover glass 5 upper surfaces.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, and its structure comprises:
The cup-shaped culture dish 2 of culture dish lid 1 is stamped in one upper surface, and the bottom surface of described cup-shaped culture dish 2 is provided with two apertures;
One is positioned at the vacuum chamber 9 of described cup-shaped culture dish 2 lower ends, and the lower surface of described vacuum chamber 9 is transparency cover slides 5;
The lower surface of two apertures on the bottom surface of described cup-shaped culture dish 2 is equipped with biocompatible flexible film 4 (as pellosil) respectively;
One links to each other with described vacuum chamber 9, and breather line 7 and vacuum extractor 8 that described vacuum chamber 9 is vacuumized.
Comprise in the present embodiment that one is arranged on the microscope (not shown) of described vacuum chamber 9 belows in order to the Real Time Observation cell.
Aperture on the bottom surface of described cup-shaped culture dish 2 is a circular port, also can be slotted eye, square hole, slot or polygonal hole;
But the molecule of protein or cell absorption is resisted in the subregion physical adsorption of described elastica 4 upper surfaces, and as Pluronic F108, protein or cell can only adhere to or growth in the zone that does not have physical adsorption Pluronic F108.
Metal (as the stainless steel) sidewall of sidewall 6 for having no side effect of described vacuum chamber 9 also can be side wall glass;
The upper surface of described transparency cover slide 5 scribbles the lubricant that has no side effect (such as silicone oil); The cross section of described vacuum chamber 9 is a Polygons, also can be square, rectangle, circle or oval, and its cross-sectional area is greater than the cross-sectional area sum of aperture on described cup-shaped culture dish 2 bottom surfaces.；
Described vacuum extractor 8 vacuumizes by 7 pairs of described vacuum chambers 9 of breather line, two biocompatible flexible films 4 of the lower surface of two apertures on the bottom surface of described cup-shaped culture dish 2 are pressed towards transparency cover slide 5 simultaneously, and are stretching along axles such as quilts in the two dimensional surface on cover glass 5 upper surfaces.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, and its structure comprises:
The cup-shaped culture dish 2 of culture dish lid 1 is stamped in one upper surface, and the bottom surface of described cup-shaped culture dish 2 is provided with an aperture;
One is positioned at the vacuum chamber 9 of described cup-shaped culture dish 2 lower ends, and the lower surface of described vacuum chamber 9 is transparency cover slides 5;
Biocompatible flexible film 4 (as pellosil) is equipped with in the upper surface of described aperture; With
One links to each other with described vacuum chamber 9, and breather line 7 and vacuum extractor 8 that described vacuum chamber 9 is vacuumized.
Comprise in the present embodiment that one is arranged on the microscope of described vacuum chamber 9 belows in order to the Real Time Observation cell.
Aperture on the bottom surface of described cup-shaped culture dish 2 is a square hole, also can be slot;
The subregion of described elastica 4 upper surfaces or upper surface can not be the plane, but some physical structures are arranged, and as column, also can be wavy upper surface, but and the adherent growth monolayer cell, also can stick the three-dimensional cell culture block;
The sidewall 6 of described vacuum chamber 9 is a plastic sidewalls, also can be the silica gel sidewall;
The upper surface of described transparency cover slide 5 scribbles the lubricant that has no side effect (such as silicone oil); The cross section of described vacuum chamber 9 is a rectangle, and the length of side of its minor face equals the length of side on the limit of the aperture on described cup-shaped culture dish 2 bottom surfaces in parallel;
Described vacuum extractor 8 vacuumizes by 7 pairs of described vacuum chambers 9 of breather line, described elastica 4 is pressed towards transparency cover slide 5, and along in the two dimensional surface on described cover glass 5 upper surfaces along the direction of described vacuum chamber 9 cross section major axis by single shaft stretch (Fig. 2).
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, reach the maximum tension degree when described elastica 4 is stretched to opening in vacuum chamber of the contact mouth of pipe of described breather line 7 or described breather line 7, described vacuum extractor 8 continues described vacuum chamber 9 vacuumized can not make described elastica 4 continue to be stretched (Fig. 2).
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, and can be to stop after taking out once, keep vacuum chamber in certain negative pressure state, and described elastica 4 keeps stretched state; Also can be to vacuumize discontinuously or clocklike and recover normal pressure, described elastica 4 be stretched with certain frequency and amplitude.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, as shown in Figure 1.
Aperture on the bottom surface of the cup-shaped culture dish 2 of present embodiment is a square hole, also can be slot;
The cross section of described vacuum chamber 9 is a rectangle, and the length of side of its minor face is greater than the length of side of the square hole on the bottom surface of described cup-shaped culture dish 2;
Scribble lubricant (as silicone oil) on the upper surface of described transparency cover slide 5;
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, and described elastica 4 is pressed towards described transparency cover slide 5, and are being stretched by single shaft along axle stretching such as quilt earlier in the two dimensional surface of the upper surface of described transparency cover slide 5 again.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, as shown in Figure 1.
Aperture on the bottom surface of the cup-shaped culture dish 2 of present embodiment is a square hole, also can be slot;
The cross section of described vacuum chamber 9 is I-shaped, and intermediate portion wide equals the length of side of the square aperture on the bottom surface of described cup-shaped culture dish 2;
Scribble lubricant (as silicone oil) on the upper surface of described transparency cover slide 5;
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, and described elastica 4 is pressed towards described transparency cover slide 5, and are being waited the axle stretching again along being stretched by single shaft earlier in the two dimensional surface of the upper surface of described transparency cover slide 5.
But the pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, as shown in Figure 1.
Aperture on the bottom surface of the described cup-shaped culture dish 2 of present embodiment is a square hole, also can be slot;
The cross section of described vacuum chamber 9 is I-shaped, the length of side of the square aperture on the bottom surface that is wider than described cup-shaped culture dish 2 of intermediate portion;
Scribble lubricant (as silicone oil) on the upper surface of described transparency cover slide 5;
8 pairs of described vacuum chambers 9 of described vacuum extractor vacuumize, and described elastica 4 is pressed towards described transparency cover slide 5, and along in the two dimensional surface of the upper surface of transparency cover slide 5 successively by etc. axle stretching, uniaxial extension, etc. axle stretch.
The pair cell of present embodiment applies the cell culture apparatus of mechanical stimulation, apply the cell culture apparatus of mechanical stimulation and cell culture apparatus that embodiment 4 described pair cell apply mechanical stimulation by an embodiment 1 described pair cell and link together by breather line 7 and form a machinery, vacuumize control by a vacuum extractor 8.
Claims (13)
1. a pair cell applies the cell culture apparatus of mechanical stimulation, comprising: the cup-shaped culture dish (2) of culture dish lid (1) is stamped in a upper surface; One is positioned at the vacuum chamber (9) of described cup-shaped culture dish (2) lower end; One vacuum extractor (8) that links to each other with described vacuum chamber (9) and described vacuum chamber (9) is vacuumized; It is characterized in that:
The bottom surface of described cup-shaped culture dish (2) is provided with aperture;
The lower surface of described vacuum chamber (9) is a transparency cover slide (5);
The upper surface of described aperture or vacuum chamber (9) end face corresponding with described aperture are equipped with biocompatible flexible film (4).
2, apply the cell culture apparatus of mechanical stimulation by the described pair cell of claim 1, it is characterized in that, clamp a transition medium layer (3) that has an aperture between described cup-shaped culture dish (2) and the described vacuum chamber (9), the aperture on the described transition medium layer (3) is corresponding with the aperture on described cup-shaped culture dish (2) bottom surface; Aperture on the described transition medium layer (3) is equal to, or greater than the aperture on described cup-shaped culture dish (2) bottom surface.
3, apply the cell culture apparatus of mechanical stimulation by claim 1 or 2 described pair cells, it is characterized in that, comprise that also one is arranged on the microscope of described vacuum chamber (9) below in order to the Real Time Observation cell.
4, apply the cell culture apparatus of mechanical stimulation by the described pair cell of claim 1, it is characterized in that, the quantity of the last aperture of the quantity of aperture and described transition medium layer (3) equates that its quantity is at least 1 on described cup-shaped culture dish (2) bottom surface.
5, apply the cell culture apparatus of mechanical stimulation by the described pair cell of claim 2, it is characterized in that aperture on described cup-shaped culture dish (2) bottom surface and the aperture on the described transition medium layer (3) are circular port, slotted eye, square hole, slot or polygonal hole.
6, apply the cell culture apparatus of mechanical stimulation by claim 1 or 2 described pair cells, it is characterized in that, chemistry or physical modification are carried out in the subregion of described elastica (4) upper surface or upper surface.
7, apply the cell culture apparatus of mechanical stimulation by claim 1 or 2 described pair cells, it is characterized in that, the sidewall (6) of described vacuum chamber (9) is silica gel sidewall, side wall glass, plastic sidewalls or the metal sidewall that has no side effect.
8, apply the cell culture apparatus of mechanical stimulation by the described pair cell of claim 1, it is characterized in that, the upper surface of described transparency cover slide (5) scribbles the lubricant that has no side effect.
9, apply the cell culture apparatus of mechanical stimulation by claim 1 or 2 described pair cells, it is characterized in that, the cross section of described vacuum chamber (9) is circle, ellipse, square, rectangle, I shape or Polygons.
10, apply the cell culture apparatus of mechanical stimulation by claim 1,2 or 4 described pair cells, it is characterized in that, the cross-sectional area of described vacuum chamber (9) is greater than the cross-sectional area sum of aperture on described cup-shaped culture dish (2) bottom surface.
11, apply the cell culture apparatus of mechanical stimulation by claim 1,2 or 4 described pair cells, it is characterized in that, the cross-sectional area of described vacuum chamber (9) is gone up the cross-sectional area sum of aperture greater than described transition medium layer (3).
12, apply the cell culture apparatus of mechanical stimulation by claim 1 or 2 described pair cells, it is characterized in that, described vacuum extractor (8) vacuumizes described vacuum chamber (9) by breather line (7); The port of described breather line (7) stretches into the interior length of described vacuum chamber (9) less than the distance between the sidewall (6) of the sidewall described vacuum chamber of distance (9) of outermost aperture on described cup-shaped culture dish (2) bottom surface.
13, apply the cell culture apparatus of mechanical stimulation by the described pair cell of claim 1, it is characterized in that, the cell culture apparatus that described pair cell applies mechanical stimulation is a plurality of, the cell culture apparatus that a plurality of pair cells apply mechanical stimulation composes in parallel a machinery, a described vacuum extractor (8) links to each other with all vacuum chambers (9) of described machinery simultaneously, and all vacuum chambers (9) are vacuumized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820108541 CN201261787Y (en) | 2008-06-13 | 2008-06-13 | Cell cultivation apparatus for exerting mechanical stimulation on cell |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820108541 CN201261787Y (en) | 2008-06-13 | 2008-06-13 | Cell cultivation apparatus for exerting mechanical stimulation on cell |
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| CN201261787Y true CN201261787Y (en) | 2009-06-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200820108541 Expired - Lifetime CN201261787Y (en) | 2008-06-13 | 2008-06-13 | Cell cultivation apparatus for exerting mechanical stimulation on cell |
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| CN (1) | CN201261787Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603005B (en) * | 2008-06-13 | 2011-08-31 | 国家纳米科学中心 | Cell culture device for applying mechanical stimulation to cells |
| CN103184144A (en) * | 2013-03-22 | 2013-07-03 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
-
2008
- 2008-06-13 CN CN 200820108541 patent/CN201261787Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603005B (en) * | 2008-06-13 | 2011-08-31 | 国家纳米科学中心 | Cell culture device for applying mechanical stimulation to cells |
| CN103184144A (en) * | 2013-03-22 | 2013-07-03 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
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Granted publication date: 20090624 Effective date of abandoning: 20080613 |