CN100514059C - Sinusoidal tensile cell loader - Google Patents
Sinusoidal tensile cell loader Download PDFInfo
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- CN100514059C CN100514059C CNB2006100951445A CN200610095144A CN100514059C CN 100514059 C CN100514059 C CN 100514059C CN B2006100951445 A CNB2006100951445 A CN B2006100951445A CN 200610095144 A CN200610095144 A CN 200610095144A CN 100514059 C CN100514059 C CN 100514059C
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Abstract
A kind of sine pulls to stretch cell to add to carry device, involve a medical science experiment instrument in of the cell mechanics add to carry device. The invention mainly includes a machine, wheel gear deceleration to adjust soon electrical engineering, number to show to adjust soon a machine, crank a slippery piece organization and pulls to stretch sport organization. The crank slips a piece of organization mainly from adjust a pole, the song arm slippery piece, crank and pull pole composing; Pull to stretch sport organization mainly from glide a support, fix support, direction pole, pioneer pole piece and behind lead a pole piece composing. Because the invention has structure simple, operate simple, maintenance convenience, the cost is cheap, circulating steady, sex price ratio high, the cell is subjected to dint even, can regulate to pull to stretch characteristics, such as range and frequency...etc., pull to stretch distance can the convenience regulates within the scope of 0~25 mm, can satisfy various cell which stick a wall growth to carry on the mechanics incitement which pulls to stretch to add to carry to respond to a research, so the invention can used to the cell mechanics within instrument of the medical science experiment to add to carry device extensively, being applicable to funds and profession to operate the think factory that the personnel lacks specially extensively applied.
Description
Technical field
The invention belongs to the biomechanics field of engineering technology, particularly the sinusoidal charger of the cyto-mechanics in the medical experiment instrument.
Technical background
Along with the development of organizational project and biomedical engineering, cyto-mechanics, organizational project etc. has become the front subject of modern biology development.In order to study the influence of various physics, biochemical factor, must solve all how pair cell carries out the problem that mechanics loads to tissue.In existing cell loader, as publication number is " four point bending cell mechanics loader " patent of CN1425905A, disclosed is the order that stepper motor is carried out CPU, thereby drive staking punch and move various running speeds, displacement, staking punch makes beam be out of shape the back between four strong points of left and right symmetry and the beam surface area is changed, thereby makes the cell generation deformation of adherent growth on the culture plate that is arranged between four strong points of beam.The advantage of this load apparatus is that control operation finished by CPU, realize that by stepper motor pair cell carries out multi-form stretching or compression, but, the subject matter that this load apparatus exists is: 1. the distortion of beam between four strong points is uneven, unavoidably will cause the cell deformation of adherent growth on the culture plate inhomogeneous.2. the distortion between four strong points is by the displacement decision of staking punch and through the lintel intermediate conversion, makes cytomorphosis directly perceived and can bring error like this.3. this load apparatus can not be realized cell microexamination under stretching or compressive state.
Summary of the invention
The objective of the invention is weak point at existing cell loader, a kind of sinusoidal tensile cell loader is provided, have simple in structure, easy and simple to handle, with low cost, reliable operation, operate steadily, the cost performance height, cell is stressed evenly, characteristics such as energy adjustment criteria sinusoidal tensile amplitude and frequency.
The object of the present invention is achieved like this: a kind of sinusoidal tensile cell loader mainly comprises frame, gear reduction buncher, digital display speed regulator, slider-crank mechanism and extensional motion mechanism.Gear reduction buncher and digital display speed regulator are arranged on the rear end of frame, and slider-crank mechanism and extensional motion mechanism are arranged on the front end of frame, separate by the frame installing plate therebetween.The gear reduction buncher is connected by the outer indoor 220 volts of power supplys of lead and device, its output shaft passes hole on the frame installing plate and is fixedly connected in the blind hole of crank rear end of slider-crank mechanism, crank is rotated together with the gear reduction buncher and provides power for device.The digital display speed regulator is connected with the gear reduction buncher by lead, demonstrates the rotating speed of gear reduction buncher in real time, and comes the rotating speed of adjusting gear deceleration buncher by the electric signal that the speed governing button of twisting on the digital display speed regulator is exported.Fan is arranged on the cover plate of frame rear end portion, and the indoor 220 volt power supplys outer by lead and device are connected, and gear reduction buncher and digital display speed regulator are dispelled the heat.Slider-crank mechanism mainly constitutes by adjusting screw rod, the slide block of cranking arm, crank and pull bar, the rotary power of gear reduction buncher is passed to sliding support make the standard sine rectilinear motion, and the offset of sinusoidal stretch range is regulated, and loads stretching so that pair cell carries out sine.Medial end portions at crank is provided with a through hole, the adjustment screw rod passes through hole and is movably connected on the crank, crank arm slide block the rear end the through hole of crank medial end portions with adjust screw rod and be connected, the square hole of the slide block middle-end of cranking arm is through on the square beam of crank front end and is slidingly connected, twist the adjustment screw rod and can regulate the eccentric position of slide block on crank of cranking arm, thereby realize regulating the amplitude of sinusoidal tensile.Slotted hole and pull bar that the axle journal of the slip front of cranking arm passes the pull bar right-hand member are slidingly connected, and the pull bar other end passes positioning adapter fixedlys connected with sliding support by bearing pin.Extensional motion mechanism mainly is made of sliding support, fixed support, guide pole, preceding guide rod piece and back-guiding rod piece, and the culture membrane that is attached with cell is stretched.The two ends of sliding support are connected with the end locking of left and right two guide poles by stop screw respectively, and can be free to slide on two guide poles.Support bracket fastened two ends are connected with the other end locking of left and right two guide poles by stop screw respectively, and can running fix on two guide poles by stop screw.The two ends of left and right two guide poles are fixed on the forward and backward guide rod piece by screw respectively, fixedly connected with frame by gib screw respectively in the two ends of forward and backward guide rod piece.Therefore two stands and two guide poles and two guide rod pieces and frame constitute firm rigid foundation, can guarantee the steady of drawing process.An end that is attached with the culture membrane of cell is pressed on support bracket fastened lower end by clamping plate, and the other end is pressed on the lower end of sliding support by clamping plate, is placed with double dish on the frame below the culture membrane.The culture membrane that is attached with cell carries out unilateral stretching by sliding support under the effect of gear reduction buncher, and can pull down extensional motion mechanism from frame easily and carry out microexamination by backing out gib screw.
After the present invention adopts technique scheme, have simple in structure, easy and simple to handle, operate steadily, the cost performance height, the stressed characteristics such as evenly of cell can be regulated sinusoidal tensile amplitude and frequency, its stretching distance is can be in 0～25mm scope convenient to be regulated, and can satisfy the mechanical stimulation response investigations that the cell of various adherent growth stretches and loads.This charger can be regulated the sinusoidal tensile frequency by the digital display speed regulator easily, and regulate the eccentric distance of slide block on crank of cranking arm according to the scale on the crank and regulate the sinusoidal tensile amplitude, make manipulation process simple, regulate easyly, operating personnel need not to wait amplitude and the frequency of professional training by manual operation adjusting sinusoidal tensile through programming.This charger adopts the sinusoidal tensile load mode, biological tissue and cell are being carried out in the mechanical stimulation response investigations, this be the most basic also be the most frequently used load mode, be converted to the standard sine rectilinear motion by adopting slider-crank mechanism to rotatablely move, got rid of the danger that the electronic switch mode is subjected to electromagnetic interference (EMI), made the device work more reliable, the course of work is directly perceived, abnormal conditions in the running can in time be got rid of, and are easy to maintenance.When this charger dynamic observes in experimentation, can directly extensional motion mechanism integral body on the frame be torn open by gib screw and move to microscopically and observe, make cell not break away from nutrient solution, reduce the contaminated possibility of culture membrane and cell greatly.The little interference by environment of this charger, with low cost, can be widely used as the cyto-mechanics charger in the medical experiment instrument, be specially adapted to research institution's widespread use of the shortage of capital and professional operating personnel.
Description of drawings
Fig. 1 is a structural drawing of the present invention;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 is the structural drawing of the slider-crank mechanism of the present invention's amplification;
Fig. 4 is the cut-open view of Fig. 3 at the A-A place.
Among the figure: 1 one gear reduction bunchers, 2-digital display speed regulator, 3-fan, 4-cover plate, 5-frame, 6-adjustment screw rod, 7-crank, the 8-slide block of cranking arm, 9-pull bar, 10-preceding guide rod piece, 11-bearing pin, 12-sliding support, 13-guide pole, 14-culture membrane, 15-double dish, 16-clamping plate, 17-fixed support, 18-stop screw, 19-back-guiding rod piece, 20-gib screw, 21-positioning adapter, 22-frame installing plate.
Embodiment
Further specify the present invention below in conjunction with embodiment.
Shown in Fig. 1～4, a kind of sinusoidal tensile cell loader mainly comprises frame 5, gear reduction buncher 1, digital display speed regulator 2, slider-crank mechanism and extensional motion mechanism.Gear reduction buncher 1 and digital display speed regulator 2 are fixed in the rear end of frame 5 respectively by screw, slider-crank mechanism and extensional motion mechanism are arranged on the front end of frame 5, separate by frame installing plate 22 therebetween.The power of gear reduction buncher 1 is 15W, the indoor 220 volt power supplys outer by lead and device are connected, its output shaft passes hole on the frame installing plate 22 and is fixedly connected on by key in the blind hole of crank 7 rear ends of slider-crank mechanism, makes crank 7 provide power with 1 rotation of gear reduction buncher for device.Digital display speed regulator 2 is connected with gear reduction buncher 1 by lead, demonstrates the rotating speed of gear reduction buncher 1 in real time, and comes the rotating speed of adjusting gear deceleration buncher 1 by the electric signal that the speed governing button of twisting on the digital display speed regulator 2 is exported.Fan 3 is fixed in by screw on the cover plate 4 of frame 5 rearward end, and the indoor 220 volt power supplys outer by lead and device are connected, and gear reduction buncher 1 and digital display speed regulator 2 are dispelled the heat.Slider-crank mechanism mainly by adjust screw rod 6, crank 7, crank arm slide block 8 and pull bar 9 constitute, the rotary power of gear reduction buncher 1 is passed to sliding support 12 make sinusoidal rectilinear motion, and the offset of sinusoidal stretch range is regulated, and loads stretching so that pair cell carries out sine.Medial end portions at crank 7 is provided with a through hole, adjustment screw rod 6 passes through hole and is movably connected on the crank 7 by clamp, the crank arm rear end of slide block 8 is connected with the screw thread pair of adjusting screw rod 6 in the through hole of crank 7 medial end portions, the square hole of slide block 8 middle-ends of cranking arm is through on the square beam of crank 7 front ends and is slidingly connected, twist adjustment screw rod 6 and can regulate the eccentric position of slide block 8 on crank 7 of cranking arm, thereby realize regulating the amplitude of sinusoidal tensile.Slotted hole and pull bar 9 that the axle journal of slide block 8 front ends of cranking arm passes pull bar 9 right-hand members are slidingly connected, and pull bar 9 other ends pass positioning adapter 21 fixedlys connected with sliding support 12 by bearing pin 11.Extensional motion mechanism mainly is made of sliding support 12, fixed support 17, guide pole 13, preceding guide rod piece 10 and back-guiding rod piece 19, and the culture membrane 14 that is attached with cell is stretched.The two ends of sliding support 12 are connected with the end locking of left and right two guide poles 13 by stop screw 18 respectively, and can be free to slide on two guide poles 13.The two ends of fixed support 17 are connected with the other end locking of left and right two guide poles 13 by stop screw 18 respectively, and can running fix on two guide poles 13 by stop screw 18.The two ends of left and right two guide poles 13 respectively by screw retention on preceding guide rod piece 10, back-guiding rod piece 19, fixedly connected with frame 5 by gib screw 20 respectively in the two ends of preceding guide rod piece 10, back-guiding rod piece 19.Therefore sliding support 12, fixed support 17 and two guide poles 13 and preceding guide rod piece 10, back-guiding rod piece 19 constitute firm rigid foundation with frame 5, can guarantee the stable of drawing process.The culture membrane 14 that is attached with cell is by clamping plate 16, and the one end is pressed on the lower end of fixed support 17, and the other end is pressed on the lower end of sliding support 12, and is placed with double dish 15 on the frame below the culture membrane 14 5.The culture membrane 14 that is attached with cell carries out unilateral stretching by sliding support 12 under the effect of gear reduction buncher 1, and can pull down extensional motion mechanism from frame 5 easily and carry out microexamination by backing out gib screw 20.
The course of work of sinusoidal tensile cell loader: rotate the speed governing button adjusting joint sinusoidal tensile frequency on the digital display speed regulator 2, digital display speed regulator 2 output corresponding electric signal are given gear reduction buncher 1, gear reduction buncher 1 drives crank 7 and rotates together, crank 7 rotations drive the slide block 8 eccentric rotations of cranking arm on it, the slide block 8 of cranking arm on the crank 7 is adjusted screw rod 6 adjusting eccentric distances by rotating, thereby realize regulating stretch range, the slide block 8 eccentric rotation pushing drawing rods 9 of cranking arm are made sinusoidal rectilinear motion, pull bar 9 moves along guide pole 13 by bearing pin 11 traction sliding supports 12, culture membrane 14 1 ends are with sliding support 12 motions, the other end then is locked on the guide pole 13 motionless with fixed support 17, just be stretched attached to the cell on the culture membrane 14 like this and make sinusoidal rectilinear motion.
Experimental result and analysis: sinusoidal tensile cell loader is in bioengineering institute of University Of Chongqing laboratory, as the orientation research of colony's hepatoma carcinoma cell and single cancer cell.Sinusoidal tensile cell loader cleans up before installing, the part that contacts with nutrient solution takes out the back and cleans with tri-distilled water with 75% alcohol solution dipping 12h, ultraviolet ray irradiation 3h, other parts are with 75% ethanolic solution wiping, ultraviolet ray irradiation 1h again after assembling finishes.Afterwards the PU film is installed in and inoculates the HEPG-2 hepatoma carcinoma cell between two stands after the tensioning, add nutrient solution then and surpass PU film 2～3mm, put into 5%CO
2In the incubator, observe adherent situation behind the 24h.Cell attaches after the growth well, the cancer cell that is inoculated on the PU film is imposed stretch range 10%, the cycle sinusoidal tensile of frequency 0.5Hz.Not timing afterwards experiment is observed.
The process contrast is found by experiment, and growth kitchen range round under colony's hepatoma carcinoma cell original state gradually becomes microscler in the response of strain, and its new orientation is vertical with the drawing stress direction.Readjusting of shape and orientation all takes place in single cancer cell under the influence of cyclic strains, it is longer that cell becomes, and cell orientation is almost vertical with the stress direction that applies, and cell orientation is adjusted to and just kept vertical direction after principal strain power is vertical.
Claims (2)
1. sinusoidal tensile cell loader, comprise frame (5), it is characterized in that also having gear reduction buncher (1) and digital display speed regulator (2) to be arranged on the rear end of frame (5), slider-crank mechanism and extensional motion mechanism are arranged on the front end of frame (5), separate by frame installing plate (22) therebetween, gear reduction buncher (1) is connected by the outer indoor 220 volts of power supplys of lead and device, its output shaft passes hole on the frame installing plate (22) and is fixedly connected in the blind hole of crank (7) rear end of slider-crank mechanism, digital display speed regulator (2) is connected with gear reduction buncher (1) by lead, fan (3) is arranged on the cover plate (4) of frame (5) rearward end, the indoor 220 volt power supplys outer by lead and device are connected, slider-crank mechanism is by adjusting screw rod (6), the slide block (8) of cranking arm, crank (7) and pull bar (9) constitute, medial end portions at crank (7) is provided with a through hole, adjustment screw rod (6) passes through hole and is movably connected on the crank (7), crank arm slide block (8) the rear end the through hole of crank (7) medial end portions with adjust screw rod (6) and be connected, the square hole of slide block (8) middle-end of cranking arm is through on the square beam of crank (7) front end and is slidingly connected, slotted hole and pull bar (9) that the axle journal of slide block (8) front end of cranking arm passes pull bar (9) right-hand member are slidingly connected, pull bar (9) other end passes positioning adapter (21) fixedlys connected with sliding support (12) by bearing pin (11), extensional motion mechanism is by sliding support (12), fixed support (17), guide pole (13), preceding guide rod piece (10) and back-guiding rod piece (19) constitute, the two ends of sliding support (12) are respectively by a stop screw (18) and a left side, the one end locking of right two guide poles (13) connects, the two ends of fixed support (17) are respectively by a stop screw (18) and a left side, the other end locking of right two guide poles (13) connects, and can be in upward running fix of two guide poles (13) by stop screw (18), a left side, guide rod piece (10) before the two ends of right two guide poles (13) are fixed in by screw respectively, on the back-guiding rod piece (19), front and back guide rod piece (10,19) fixedly connected with frame (5) by gib screw (20) respectively in two ends, an end that is attached with the culture membrane (14) of cell is pressed on the lower end of fixed support (17) by clamping plate (16), the other end is pressed on the lower end of sliding support (12) by clamping plate (16), is placed with double dish (15) on the frame (5) of culture membrane (14) below.
2. according to right 1 described sinusoidal tensile cell loader, it is characterized in that gear reduction buncher (1) and digital display speed regulator (2) are fixed in the rear end of frame (5) respectively by screw, the power of gear reduction buncher (1) is 15W, fan (3) is fixed on the cover plate (4) of frame (5) rearward end by screw, adjustment screw rod (6) passes through hole and is movably connected on the crank (7) by clamp, and the rear end of the slide block of cranking arm (8) is connected with adjustment screw rod (6) screw thread pair in the through hole of crank (7) medial end portions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100951445A CN100514059C (en) | 2006-09-22 | 2006-09-22 | Sinusoidal tensile cell loader |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100951445A CN100514059C (en) | 2006-09-22 | 2006-09-22 | Sinusoidal tensile cell loader |
Publications (2)
| Publication Number | Publication Date |
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| CN1932511A CN1932511A (en) | 2007-03-21 |
| CN100514059C true CN100514059C (en) | 2009-07-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2006100951445A Expired - Fee Related CN100514059C (en) | 2006-09-22 | 2006-09-22 | Sinusoidal tensile cell loader |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN101649291B (en) * | 2009-08-25 | 2011-12-21 | 四川大学 | Extension and compression device of multi-unit cells |
| CN101906379A (en) * | 2010-07-09 | 2010-12-08 | 东南大学 | Device for precisely stretching visual cells under simulated in vivo environment |
| CN102680176B (en) * | 2012-05-24 | 2015-10-14 | 中国石油天然气集团公司 | A kind of tubing stress corrosion (cracking) test machine in kind |
| US9617507B2 (en) | 2012-10-24 | 2017-04-11 | IonOptix LLC | Apparatus and method for culturing cells and tissue |
| CN103184144B (en) * | 2013-03-22 | 2015-04-29 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
| CN105154331B (en) * | 2015-09-28 | 2017-11-03 | 苏州大学 | Cell tension and compression equipment |
| CN106867888A (en) * | 2017-03-20 | 2017-06-20 | 北京理工大学 | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time |
| FR3064359B1 (en) | 2017-03-21 | 2021-04-16 | Centre Nat Rech Scient | DEVICE FOR THE MECHANICAL CHARACTERIZATION OF AN ELEMENT OF INTEREST, FOR EXAMPLE AN OVOCYTE |
| CN109628305A (en) * | 2018-12-12 | 2019-04-16 | 北京理工大学 | The multichannel differential pulling device for answering mechanics response mechanism to study for external aixs cylinder |
| CN110904090B (en) * | 2019-11-25 | 2021-10-08 | 东华大学 | Dynamic cell culture method and culture device simulating in-vivo force-electricity microenvironment |
| CN111718835B (en) * | 2020-06-12 | 2023-05-26 | 西安工业大学 | Cell tissue mechanics simulator |
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2006
- 2006-09-22 CN CNB2006100951445A patent/CN100514059C/en not_active Expired - Fee Related
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| CN1932511A (en) | 2007-03-21 |
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