CN103355187B - Animal model manufacturing device with magnetic field generating device - Google Patents
Animal model manufacturing device with magnetic field generating device Download PDFInfo
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Abstract
An animal model manufacturing device with a magnetic field generating device comprises a box body, a magnetic field generating device, a magnetic field detecting device, a temperature detecting and controlling device, a voltage control device, a ventilation device, a lighting device and a cooling device; the magnetic field generating device is placed in the box body and is provided with a cavity used for accommodating an experimental animal or an experimental animal feeding container; the side wall of the box body is provided with a door used for putting in or taking out the experimental animal or the experimental animal feeding container; the cooling device makes contact with the magnetic field generating device and is used for cooling the magnetic field generating device; the ventilation device and the lighting device are respectively used for ventilation and lighting for the magnetic field generating device; the voltage control device is connected with the magnetic field generating device and is used for controlling the magnetic field intensity generated by the magnetic field generating device. According to the animal model manufacturing device, both enough magnetic field intensity and magnetic field space suitable for survival of the experimental animal are provided. Furthermore, temperature, lighting, ventilation and the like can be controlled, and the animal model manufacturing device can be used for a long time.
Description
Technical field
The present invention relates to animal model preparation field, be specifically related to the animal model preparation facilities with field generator for magnetic.
Background technology
Along with the progress of society, the development of electric utility, the life increasing extent of power frequency high voltage equipment settlement and people is near, is exposed in power frequency electromagnetic field whether can affect health for a long time, causes the common concern of the public.There is voltage just to have electric field, have electric current just to have electromagnetic field, therefore, all there is electric field around socket, switch, the power line that is connected with socket, near the electrical appliance used and transmission line thereof, all there is power frequency electromagnetic field.The power frequency magnetic field exposure limits that China's (GB exposure draft) provides is occupational exposure 78 μ T, the public exposes as 22 μ T, and the professional exposure limit of European Union and Australia etc. is defined as 500 μ T more, but great exposure strength and dosage can damage human body, do not have definite final conclusion actually.
The earth has earth's magnetic field about (about 3 × 10
-7t), tellurian life has also adapted to the existence in earth's magnetic field in evolution process, and some biology also make use of earth's magnetic field dexterously.Along with the development of science and technology, people get more and more to the application in magnetic field, and numerous Magnetotherapeutic health product also emerges in multitude, as magnetic pad, magnetic pillow, low-frequency pulse Magnetotherapeutic apparatus etc.Magnetic field has found many results for the treatment of in clinical practice, as improved microcirculation in human body, promoting union, preventing and treating osteoporosis etc.Whether people, while effectively utilizing magnetic field, will understand magnetic field more urgently and be harmful to biology, and it are on the impact of environment.
Reproduction due to the mankind relates to the quality of population, low frequency electromagnetic field, particularly power frequency electromagnetic field wherein, especially receives publicity on the impact of human reproduction.Develop various magnetic field generating device at present, to observe the impact of magnetic exposure on laboratory animal physiological function, particularly observe its impact on animals' reproduction function.
Such as, CXLY-II type pulsed magnetic field generator, this instrument can produce frequency 0 ~ 100Hz, the continuously adjustable pulsed magnetic field of magnetic field intensity 150 ~ 800mT, can work long hours, the object of the multiple volume profiles such as mouse, Tissue Culture Plate, Micro-Organism Culture Dish can be put in magnetic fields region (85mm × 40mm × 25mm), can be used for research magnetic field to the effect of the multiple organism effects such as animal, cell, bacterium.But the magnetic fields space of this magnetic field generator is less, the laboratory animal such as mouse free living in this limited space can not be held.That is, when testing, can only be that laboratory animal is placed in the device (and fixing) a few hours, to carry out magnetic exposure to it, afterwards laboratory animal being taken out, after raising, then put into magnetic field generating device, so repeatedly.This not only takes time and effort, and be not under free movement state due to these laboratory animal and be exposed in alternating magnetic field environment constantly for a long time, the situation continuing to be exposed to power frequency magnetic field in this and real life environments under free movement state is not for a long time inconsistent, and therefore obtained animal model is not optimum.
In a word, need one to breed experimental animals at present wherein, and make device prepared by the magnetic exposure animal model that laboratory animal can be freely movable in magnetic fields space.
Summary of the invention
The object of this invention is to provide a kind of can breeding experimental animals and the animal model preparation facilities with field generator for magnetic making that laboratory animal can be freely movable wherein wherein.
For achieving the above object, the invention provides a kind of animal model preparation facilities, comprise casing, field generator for magnetic, detector for magnetic field, temperature detection and regulation device and voltage-operated device, it is characterized in that:
Described preparation facilities also comprises ventilation unit, lighting device and cooling device;
Described field generator for magnetic is arranged in described casing and has the cavity for placing laboratory animal or experimental animal feeding container;
The sidewall of described casing has door, and described door is used for putting into or take out laboratory animal or experimental animal feeding container;
Described cooling device contacts with described field generator for magnetic, for cooling described field generator for magnetic;
Described ventilation unit and lighting device are respectively used to ventilate and illumination for described field generator for magnetic provides; And
Described voltage-operated device is connected with described field generator for magnetic, for controlling the magnetic field intensity that described field generator for magnetic produces.
Preferably, described casing is the casing of armoured magnetic field, and described casing is roughly cuboid, and six walls of casing are made by iron plate, and four sidewalls are provided with ventilating opening, and described door is arranged in described four sidewalls with on the sidewall of described field generator for magnetic axes normal.
Preferably, described field generator for magnetic is solenoid, and the described cavity for placing laboratory animal or experimental animal feeding container is described solenoid inner chamber.
Preferably, described detector for magnetic field comprises magnetic field display unit on the front side wall being arranged in described casing and is positioned at the magnet field probe of the cavity for placing laboratory animal or experimental animal feeding container of described field generator for magnetic.
Preferably, the sidewall of described casing is metal sidewall, for armoured magnetic field, stops magnetic field to the interference of staff and control-animal.
Preferably, described temperature detection and the regulation device temp probe that comprises temperature indicator and be connected with temperature indicator.
Preferably, described ventilation unit is exhaust fan, and described exhaust fan is arranged on the sidewall of described casing.
Preferably, described lighting device comprises illuminating lamp and luminous lamp switch controller, and described luminous lamp switch controller is used for illuminating lamp described in loop control and opens, and realizes the simulation at daytime and night.
Preferably, described cooling device comprises the cooling tube be wrapped on described field generator for magnetic, the pump installation be connected with cooling tube and refrigerant storage container, and described cooling tube, pump installation and refrigerant storage container form cooling circuit.
Preferably, the described cavity size for breeding experimental animals of described field generator for magnetic can hold normal rat rearing-box and/or standard mice rearing-box, described normal rat rearing-box is of a size of (380 ± 20) × (325 ± 20) × (180 ± 20) mm, and described standard mice rearing-box is of a size of (310 ± 20) × (200 ± 20) × (140 ± 20) mm.
Preferably, described illuminating lamp is LED.
Preferably, described cooling tube is made up of copper metal, and described cooling agent is water.
Animal model preparation facilities of the present invention can prepare the animal model that can be used in analysis of magnetic field action and affect the fertility of animals.And, this animal model preparation facilities can further provide the magnetic field space being suitable for laboratory animal existence while providing enough magnetic field intensity, but also temperature controllable, illumination, air circulation etc., and can use for a long time, make it possible to breed experimental animals under the condition of access expansion.In addition, owing to have employed shielding design, inner AC magnetic field is effectively shielded, ensure experimenter not by magnetic interference.
Accompanying drawing explanation
Fig. 1 is the structural representation with an embodiment of the animal model preparation facilities of field generator for magnetic of the present invention;
Fig. 2 is the structural representation of an embodiment of the animal model preparation facilities of Fig. 1;
Fig. 3 is the structural representation of the biopsy cavity marker devices of the animal model preparation facilities of Fig. 2, for clarity sake, and clipped structure;
Fig. 4 shows rat and to be in heat the typical vaginal smear of different phase, wherein: dioestrus (A) mainly comprises leucocyte and mucus; The a large amount of karyocytes of proestrum (B) and cornified vaginal epithelial cell are also deposited; Oestrus (C) mainly cornified vaginal epithelial cell; Oestrus the later stage (D) karyocyte and a large amount of leucocyte deposit, each vaginal smear amplifies 100 times of observations all under the microscope and takes pictures.
Embodiment
Below with reference to accompanying drawing, preferred embodiment of the present invention is described in detail, so that clearer understanding objects, features and advantages of the present invention.It should be understood that embodiment shown in the drawings is not limitation of the scope of the invention, and the connotation just in order to technical solution of the present invention is described.Part identical in figure uses the same reference numerals to represent.
Fig. 1 is the structural representation with the animal model preparation facilities of field generator for magnetic of the present invention.As shown in Figure 1, animal model preparation facilities 100 comprises casing 1, field generator for magnetic 2 (i.e. solenoid), detector for magnetic field (14,18), temperature detection and regulation device (11,12), voltage-operated device 15, cooling device (3,4,5,6), lighting device (9,10) and ventilation unit (7,8).
During work, laboratory animal by cooling device 3 around solenoid 2 in raise.Voltage-operated device 15 controls the voltage strength of solenoid 2, and then controls the intensity in the magnetic field that solenoid 2 produces, to obtain the magnetic field needed for experiment.Cooling device, when solenoid works or when temperature detection and regulation device detect that solenoid temperature exceedes predetermined value, for solenoid 2 provides cooling, thus ensures that solenoid 2 can work long hours.Lighting device realizes the simulation at daytime and night by switch control rule, and the laboratory animal for raising in solenoid 2 together with ventilation unit provides closer to self-sow environment.
Fig. 2 and Fig. 3 illustrates the structural representation of an embodiment of the animal model preparation facilities 100 of Fig. 1.As Figure 1-3, in the present embodiment, casing 1 is roughly cuboid, and six walls of casing 1 are all made up of iron plate, wherein, four sidewalls all have louver (or hole), are beneficial to air circulation.The rear wall (or on other sidewalls) of casing is provided with the exhaust fan 8 be connected with controller for air exhaust fan 7, and is provided with the hole passed through for connecting line and cooling tube 3 (hereafter will be described in more detail).Preferably, bottom casing 1, be also provided with roller (not shown), to facilitate mobile box.
The front side wall of casing 1 is provided with control and display unit region 1a and door 1b, door-plate 1c, and can be put into by door 1b or take out the rearing-box 22 that laboratory animal is housed, door-plate 1c is also made up of iron plate, with armoured magnetic field better.Control and viewing area are provided with power switch 16, current sensing means 17, voltage regulator 15, magnetic field display unit 18, illumination control apparatus 9 and temperature indicator 11.
Wherein, power switch 16 is connected with general supply, voltage regulator 15 and current sensing means 17 are connected to solenoid 2, detector for magnetic field comprises the magnetic field display unit 18 on the front side wall being arranged in described casing and is positioned at the magnet field probe 14 of solenoid 2, and illumination control apparatus 9 is connected to the illuminating lamp 10 being arranged in solenoid 2.In addition, although not shown, exhaust fan 8, current sensing means 17, voltage regulator 15, magnetic field display unit 18, illumination control apparatus 9 and temperature indicator 11 etc. are all connected with power supply.
In above-mentioned animal model preparation facilities 100, by the size of voltage regulator 15 adjustable voltage, and then regulate the size of current intensity and the size of magnetic field intensity, simultaneously, the size of current intensity and magnetic field intensity shows respectively in real time in current sensing means 17 and magnetic field display unit 18, make user can observe the change in electric current and magnetic field easily, thus easily magnetic field intensity is adjusted to required value.
Illuminating lamp 10 in illumination control apparatus 9 automatic cycle control solenoid 2 is with simulate day and night.In the present embodiment, illuminating lamp is LED, the advantages such as it is long that it has the life-span, lower calorific value.
Above-mentioned field generator for magnetic is solenoid 2, can cultured space required for the magnetic field size that will produce and laboratory animal, arranges the diameter of solenoid 2, coil winding density and length thereof.
In the present embodiment, solenoidal diameter (i.e. the diameter of coil) is about 350mm, and solenoid length is about 800mm, and the homogeneous area of uniform magnetic field can be provided to be greater than
industrial frequency AC magnetic field intensity reaches 0 ~ 50.0Gs, and continuously adjustabe, accuracy reaches 2%.
The large I of helix tube inner chamber holds the rat feeding case (size is about 380 × 325 × 180mm) of two standards or the Mouse feeder case (size is about 310 × 200 × 140mm) of 4 standards.But should be understood that and can solenoidal diameter and length be set as required, to hold other experimental animal feeding casees, such as rabbit rearing-box.
In addition, due to field generator for magnetic, namely calorific value is large in the course of the work for solenoid 2, and particularly for producing high-intensity magnetic field or providing the solenoid of homogeneous area of large uniform magnetic field, calorific value is especially large, and it cannot be worked long hours.For guaranteeing to work for a long time, the outer wall of solenoid 2 is wound with cooling tube 3, cooling tube 3 and solenoid 2 electric insulation.One end of cooling tube 3 is connected to pump installation 5, and the other end is connected to the container 4 storing cooling agent, and pump installation 5 other end is also connected to container 4, forms circulation cooling circuit thus.Preferably, cooling tube 3 is made up of copper metal, and cooling agent is water.It should be noted that cooling tube 3 also contacts with solenoid 2 by modes such as stickups, as long as solenoid 2 can be cooled.
By arranging linked switch, when solenoid 2 is started working, pump installation 5 is also started working, and to lower the temperature to solenoid 2, thus ensures that solenoid 2 and animal model preparation facilities 100 can work long hours.Or when temperature detection and regulation device, when namely temp probe 12 detects that solenoidal temperature exceedes predetermined value, pump installation starts and lowers the temperature to solenoid.
The application of animal pattern and prepared animal pattern is prepared by animal model preparation facilities of the present invention
1 laboratory animal
Female and the male rat (purchased from Shanghai City western pul-Bi Kai laboratory animal Co., Ltd) of healthy SPF level SD.The normal condition of raising is that every day, light application time was 6:00 to 18:00, and animal freely drinks water and ingests, and indoor temperature control is in (20 ± 2) DEG C.
2 magnetic fields are in the experimental technique of rat
Get the female rats in 10 4 week ages, be divided into 2 groups at random.Experimental group 5, raises in field generator for magnetic inner, and maintain magnetic field intensity 1mT, every day stimulates 8 hours from 16:00 to 24:00, continues stimulation 4 weeks, for detecting oestrus and blood steroid hormone level.During magnetic exposure, animal freely can drink water, ingest, and room temperature controls at 20 ± 2 DEG C.Control group (5) is raised outer 2 meters of the field generator for magnetic in same room, the noiseless part in experiment magnetic field.
Similar with above experimental design, experimental group and control group respectively get 8 rats starting to process from 4 week age, after maintaining 4 weeks, mate with 1:1 ratio with the male mouse of normal adult.Whether next day is by having sperm to determine whether mating occurs in vaginal smear examination genital tract.The successful female rats of mating, raises separately, the pregnancy duration length of record rat, divides during 28 days puerperiums and claims to obtain female mouse and filial generation body weight.Observe embryonic development situation, record conceived number of days, newborn mouse grows and Cluth size, with the impact of analysis of magnetic field action on female mouse fecundity.
Get the male rat experimental group 8 in 8 week age at random, maintain magnetic field intensity 2mT, every day, stimulation time was by 18:00 to 06:00, totally 12 hours.Separately get 4 male rats as a control group.Continue after 60 days, put to death rat, get epididymal sperm, analyze the indexs such as sperm morphology.
The judgement of 3 female rats Each Stage of Estrus Cycles
Get rat in 4 week age, process 4 thoughtful rats through magnetic field and grow up (8 week age), then start vaginal smear examination Estrus Cycle in Rats.Concrete grammar is, morning every day, 8:30 got female mouse to be checked, routinely, gets rat vagina mucus be evenly coated in and add on the slide of aqua sterilisa with the round end glass bar of sterilizing, and conventional Giemsa staining method dyeing is observed.Reference literature, the under the microscope Oestrous Stages of qualification residing for rat.General plate coating checking continues more than two cycles.Next, be checked through about the 10:30 in the morning on the same day in oestrus, venous blood sampling 1.2ml under Rat Tongue, separated plasma is for detecting the mensuration of FSH, oestrogenic hormone and progesterone level.
4 hormone test methods
Utilize estradiol and progesterone diagnostic kit (Electrochemiluminescince, German Roche Diagnostics GmbH), according to kit operation instructions, Roche 2010 Electrogenerated chemiluminescent immunoassay instrument measures E2 and P4 concentration.
The analytical method of 5 rat spermatozoas
Rat is got its cauda epididymis immediately, removes peripheral adipose, blood and tissue juice as far as possible after putting to death; Cauda epididymidis is moved in the PBS buffer solution (2ml) containing 1%BSA.Live the side of cauda epididymis with fine forceps sub-folder, cut a vertical osculum at another side eye scissors, cauda epididymis spermatium is extruded.At 37 DEG C, cultivate 1 hour under 5%CO2 condition, mixing, draws 6 μ l suspensions, drips in counting plate for sperm (macro ruby tally, Shanghai micro-figure instrument development in science and technology Co., Ltd), counting sperm concentration and activity ratio.Motile sperm respectively in a counting visual field and spermatium quantity are to estimate sperm motility, and every this count totals of increment is greater than 200 sperms.
Use Transgreen (Shanghai Shanghai Ganfeng Bio-Tech Development Co., Ltd.) and PI (sigma) to redye and detect plasmalemmae of sperms integrality.Briefly: Transgreen and PI is mixed, adds fresh spermatozoa suspension, hatch more than 5min for 37 DEG C, get 6 μ l suspensions and drip in counting plate for sperm, observe under fluorescence microscope (LEICA, DFC360FX).Under the exciting of blue light, the complete sperm of plasma membrane sends green fluorescence, and injury of plasmalemmae sperm sends red fluorescence.Count red in the same visual field and green sperm quantity respectively, calculate survival rate, every this count totals of increment is more than 200 sperms.
6 statistical analysis methods
Observe and detect each index, recording relevant data, using each index as variable, magnetic field process and contrast are recorded as experiment grouping, insert in Excel form.Then relevant data is imported SPSS 18.0 statistical software (SPSS Inc., Chicago, IL), according to data type and distribution situation, select t inspection or Mann-Whitney U to check, obtain average and standard deviation, and significant indexes p value.Significance of difference level selects p<0.05.
Embodiment 1
Inspection magnetic field is on the impact of female rats oestrous cycle and steroid hormone blood level
Adopt the magnetic field intensity of 1mT, started lasting stimulation in rats 4 weeks from 4 week age, action time every day is from 16:00 to 24:00.Find that rat occurs restless putting 2-3 days that magnetic field space starts into, after 3 days, recover normal performance gradually.After rat being raised in magnetic field space 4 thoughtful 8 week ages, each group rat all starts to occur the oestrous cycle.By the vaginal smear method of routine, check the oestrous cycle of rat.
Result as shown in Figure 4 and Table 1.Fig. 4 shows the microscopic findings of the vaginal smear of oestrous cycle different phase.By the vaginal epithelial cell keratinization situation come off wherein, and the relative prevalence of leucocyte and mucus, determine the process in oestrous cycle.Table 1 have recorded and started magnetic field (1mT) process from 4 week age and affect situation to female rats oestrus and hormonal readiness.
Table 1. started magnetic field (1mT) process to the impact of female rats oestrus and hormonal readiness from 4 week age
Note: * marks the p value representing that the comparison between magnetic field processed group and control group is obtained by non-parametric test Mann-Whitney U mode, the comparison of the blood level of oestrogenic hormone (E2) and progestational hormone (P4) obtains p value by t inspection, suppose that variance is equal, bilateral truncation.
As can be seen from Table 1, processed group frequency of oestrusing in magnetic field significantly improves, and dioestrus is shorter, and the oestrogenic hormone in oestrus (estradiol) rises to some extent.But the blood level change of progestational hormone is little, without significant difference.
Embodiment 2
Inspection magnetic field is on the impact of female rats steroid hormone blood level and reproduction offspring
Process the female age in mouse to 8 week in 4 week age similarly.As can be seen from Table 2, the blood level of oestrogenic hormone and progesterone is not subject to remarkable impact equally, and wherein p value is all greater than 0.05.In this case, treated female rats and normal Adult male rats mating, the situation of procreate does not have the difference of statistically significant between processed group and experimental group.
Result is as shown in table 2 below, Cluth size, the average weight of every nest filial generation, and the sex ratio of every nest offspring, does not all have the difference (p>0.05) of statistically significant between magnetic field process and control group.The pregnancy duration and female mouse body weight when dividing 28 days puerperiums do not present the difference of statistically significant between magnetic field process with control group yet.
Table 2 started magnetic field (1mT) process to the impact of female rats hormonal readiness and reproduction offspring from 4 week age
Annotation: * mark represents and compares by t inspection acquisition p value between magnetic field processed group and control group, supposes that variance is equal, bilateral truncation.Grey marks the index of offspring.
Embodiment 3
8 week age, SD female rats was exposed to the impact of 5mT intensity magnetic field on blood steroid hormone level and reproduction offspring
Get SD rat 9 in 8 week age, random packet, experimental group is 8, and control group is 4.Experimental group is placed in magnetic field, and magnetic field intensity is 5mT, and every daily exposure time is 18:00 to 06:00, and light application time is 6:00 to 18:00, and rat freely drinks water and takes food; Control group is placed in outside the magnetic field of same room, and magnetic field intensity background value is 0.027 μ T.
Table 3 started magnetic field (5mT) process to the impact of female rats hormonal readiness and reproduction offspring from 8 week age
Annotation: * mark represents and compares by t inspection acquisition p value between magnetic field processed group and control group, supposes that variance is equal, bilateral truncation.Grey marks the index of offspring.
As shown in table 3.Magnetic exposure continues 60, find that in blood, estrogen level reduces, and progesterone level raises.Surprisingly, with normal with strain male copulatory, the Cluth size of offspring significantly increases (Cluth size mean value rises to 9.3 from 5.3), this and magnetic field cause the frequency of oestrusing of female rats to rise there is certain correlation, prompting magnetic field also results in the increase of number of eggs ovulated causing female rodents to be oestrused while frequency rises, thus causes Cluth size to increase.
Embodiment 4
Magnetic field is on the impact of male rat fecundity related index
Adopt the male adult rat of electromagnetic field process (8 week age) of 2mT intensity, every day, from 18:00 to 06:00, amounts to 12 hours, continues 60 days.
Result is as shown in table 4 below, and its testis and epididymis weight and control group do not have statistically significant difference; The membrane integrity of sperm concentration and sperm does not have significant difference yet; But motile sperm proportion significantly reduces in magnetic fields group, the mean value of motile sperm ratio significantly drops to 15.25% from 38.5%.
Table 4 started the impact of magnetic field (2mT) process on male rat sperm index from 8 week age
Annotation: * mark represents and compares by t inspection acquisition p value between magnetic field processed group and control group, supposes that variance is equal, bilateral truncation.Grey marks p<0.05, the index of significant difference between magnetic field process and contrast.
Embodiment 5
4 week age, SD male rat was exposed to the impact of 2mT/5mT intensity magnetic field on fecundity related index
Get SD male rat 15 in 4 week age, random packet, 2mT and 5mT magnetic exposure group is respectively 6, and control group is 3.Experimental group is placed in magnetic field, and magnetic field intensity is 2mT, and every daily exposure time is 18:00 to 06:00, and light application time is 6:00 to 18:00, and rat freely drinks water and takes food; Control group is placed in outside the magnetic field of same room, and magnetic field intensity background value is 0.027 μ T.
Result is as shown in table 5.Magnetic exposure continues 60, and result shows, from 4 week age, to be exposed to the experimental group in 2mT or 5mT magnetic field, and its sperm concentration all reduces relative to contrast.In addition, be exposed to the experimental group in 2mT or 5mT magnetic field, the ratio shared by its motile sperm all declines, more remarkable with 5mT experimental group.
Table 5 started the impact of magnetic field (2/5mT) process on male rat sperm index from 4 week age
From above-described embodiment, the animal model that can be used in analysis of magnetic field action and the fertility of animals is affected can be prepared by animal model preparation facilities of the present invention.And, this animal model preparation facilities can further provide the magnetic field space being suitable for laboratory animal existence while providing enough magnetic field intensity, and temperature controllable, illumination, air circulation etc., and can use for a long time, make it possible to breed experimental animals under the condition of access expansion.In addition, owing to have employed shielding design, inner AC magnetic field is effectively shielded, ensure experimenter not by magnetic interference.
Below described preferred embodiment of the present invention in detail, but it will be appreciated that, after having read above-mentioned instruction content of the present invention, those skilled in the art can make various changes or modifications the present invention.These equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. an animal model preparation facilities, comprises casing, field generator for magnetic, detector for magnetic field, temperature detection and regulation device and voltage-operated device, it is characterized in that:
Described preparation facilities also comprises ventilation unit, lighting device and cooling device;
Described field generator for magnetic is arranged in described casing and has the cavity for placing laboratory animal or experimental animal feeding container;
The sidewall of described casing has door, and described door is used for putting into or take out laboratory animal or experimental animal feeding container;
Described cooling device contacts with described field generator for magnetic, for cooling described field generator for magnetic;
Described ventilation unit and lighting device are respectively used to ventilate and illumination for described field generator for magnetic provides; And
Described voltage-operated device is connected with described field generator for magnetic, for controlling the magnetic field intensity that described field generator for magnetic produces.
2. animal model preparation facilities as claimed in claim 1, it is characterized in that: described casing is the casing of armoured magnetic field, described casing is cuboid, six walls of casing are made by iron plate, four sidewalls are provided with ventilating opening, and described door is arranged in described four sidewalls with on the sidewall of described field generator for magnetic axes normal.
3. animal model preparation facilities as claimed in claim 1, it is characterized in that: described field generator for magnetic is solenoid, the described cavity for placing laboratory animal or experimental animal feeding container is described solenoid inner chamber.
4. animal model preparation facilities as claimed in claim 1, is characterized in that: described detector for magnetic field comprises the magnetic field display unit on the front side wall being arranged in described casing and is positioned at the magnet field probe of the cavity for placing laboratory animal or experimental animal feeding container of described field generator for magnetic.
5. animal model preparation facilities as claimed in claim 1, is characterized in that: the temp probe that described temperature detection and regulation device comprise temperature indicator and be connected with temperature indicator.
6. animal model preparation facilities as claimed in claim 1, it is characterized in that: described ventilation unit is exhaust fan, described exhaust fan is arranged on the sidewall of described casing.
7. animal model preparation facilities as claimed in claim 1, is characterized in that: described lighting device comprises illuminating lamp and luminous lamp switch controller, and described luminous lamp switch controller is used for illuminating lamp described in loop control and opens, and realizes the simulation at daytime and night.
8. animal model preparation facilities as claimed in claim 1, it is characterized in that: described cooling device comprises the cooling tube be wrapped on described field generator for magnetic, the pump installation be connected with cooling tube and refrigerant storage container, described cooling tube, pump installation and refrigerant storage container form cooling circuit.
9. animal model preparation facilities as claimed in claim 1, it is characterized in that: the described cavity size for placing laboratory animal or experimental animal feeding container of described field generator for magnetic can hold normal rat rearing-box and/or standard mice rearing-box, described normal rat rearing-box is of a size of (380 ± 20) × (325 ± 20) × (180 ± 20) mm, and described standard mice rearing-box is of a size of (310 ± 20) × (200 ± 20) × (140 ± 20) mm.
10. animal model preparation facilities as claimed in claim 7, is characterized in that: described illuminating lamp is LED.
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| CN101583879A (en) * | 2006-11-20 | 2009-11-18 | 纳提维斯公司 | Apparatus and method for transducing an in vitro or mammalian system with a low-frequency signal |
| CN201149589Y (en) * | 2008-01-22 | 2008-11-12 | 西北工业大学 | Large displacement cell strain loading device under three-dimensional cultivation condition |
| CN101324552A (en) * | 2008-04-15 | 2008-12-17 | 中国人民解放军第四军医大学 | Design method of magnetic field biology effect experiment instrument |
| CN202761915U (en) * | 2012-04-09 | 2013-03-06 | 上海市计划生育科学研究所 | Animal model preparing device provided with magnetic field generating device |
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