CN103355187A - Animal model manufacturing device with magnetic field generating device - Google Patents
Animal model manufacturing device with magnetic field generating device Download PDFInfo
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Abstract
An animal model manufacturing device with a magnetic field generating device comprises a box body, a magnetic field generating device, a magnetic field detecting device, a temperature detecting and controlling device, a voltage control device, a ventilation device, a lighting device and a cooling device; the magnetic field generating device is placed in the box body and is provided with a cavity used for accommodating an experimental animal or an experimental animal feeding container; the side wall of the box body is provided with a door used for putting in or taking out the experimental animal or the experimental animal feeding container; the cooling device makes contact with the magnetic field generating device and is used for cooling the magnetic field generating device; the ventilation device and the lighting device are respectively used for ventilation and lighting for the magnetic field generating device; the voltage control device is connected with the magnetic field generating device and is used for controlling the magnetic field intensity generated by the magnetic field generating device. According to the animal model manufacturing device, both enough magnetic field intensity and magnetic field space suitable for survival of the experimental animal are provided. Furthermore, temperature, lighting, ventilation and the like can be controlled, and the animal model manufacturing device can be used for a long time.
Description
Technical field
The present invention relates to the animal model preparation field, be specifically related to have the animal model preparation facilities of field generator for magnetic.
Background technology
Along with the progress of society, the development of electric utility, power frequency high voltage equipment settlement and people's life scope is more and more nearer, and whether long term exposure can affect health in power frequency electromagnetic field, caused the public's common concern.There is voltage that electric field is just arranged, has electric current that electromagnetic field is just arranged, therefore, all have electric field around socket, switch, the power line that links to each other with socket, all have power frequency electromagnetic field near the electrical appliance that is using and the transmission line thereof.The power frequency magnetic field exposure limits that China's (GB exposure draft) provides is occupational exposure 78 μ T, the public exposes as 22 μ T, and the professional exposure limit of European Union and Australia etc. is defined as 500 μ T more, but great exposure strength and dosage can damage human body actually, do not have definite final conclusion.
The earth has earth's magnetic field about (about 3 * 10
-7T), tellurian life has also adapted to the existence in earth's magnetic field in the evolution process, and some biology has also utilized the earth's magnetic field dexterously.Along with the development of science and technology, people are more and more to the application in magnetic field, and numerous Magnetotherapeutic health product also emerges in multitude, such as magnetic pad, magnetic pillow, low-frequency pulse Magnetotherapeutic apparatus etc.Many results for the treatment of have been found in magnetic field in clinical practice, as improve microcirculation in human body, promote union, prevent and treat osteoporosis etc.People are when effectively utilizing magnetic field, and whether will understand more urgently magnetic field harmful to biology, with and on the impact of environment.
Because human reproduction relates to the quality of population, low frequency electromagnetic field, particularly wherein power frequency electromagnetic field especially receive publicity on human reproduction's impact.Develop at present various magnetic fields generation, to observe magnetic exposure to the impact of laboratory animal physiological function, particularly observed it to the impact of animal reproductive function.
For example, CXLY-II type pulsed magnetic field generator, this instrument can produce frequency 0～100Hz, the continuously adjustable pulsed magnetic field of magnetic field intensity 150～800mT, can work long hours, magnetic fields zone (can be put into the object of the multiple volume shapes such as mouse, Tissue Culture Plate, Micro-Organism Culture Dish, can be used for studying magnetic field to the effect of the multiple organism effects such as animal, cell, bacterium in 85mm * 40mm * 25mm).But the magnetic fields space of this magnetic field generator is less, can not hold laboratory animal free livings in this limited space such as mouse.That is, when testing, can only be that laboratory animal is placed on (and fixing) a few hours in this equipment, so that it is carried out magnetic exposure, afterwards laboratory animal is taken out, after raising, put into again the magnetic field generation, so repeatedly.This not only takes time and effort, and owing to these laboratory animal are not under free movement state and are exposed to constantly for a long time in the alternating magnetic field environment, the situation that continues for a long time to be exposed to power frequency magnetic field in this and the real life environments under free movement state is not inconsistent, and the animal model that therefore obtains is not optimum.
In a word, need at present a kind of can breeding experimental animals therein, and so that laboratory animal can be in the magnetic fields space the free device of movable magnetic exposure animal model preparation.
Summary of the invention
The purpose of this invention is to provide a kind of animal model preparation facilities with field generator for magnetic that can breed experimental animals therein and make therein freedom activity of laboratory animal.
For achieving the above object, the invention provides a kind of animal model preparation facilities, comprise casing, field generator for magnetic, detector for magnetic field, temperature detection and regulation device and voltage-operated device, it is characterized in that:
Described preparation facilities also comprises ventilation unit, lighting device and cooling device;
Described field generator for magnetic is arranged in described casing and has for the cavity of placing laboratory animal or experimental animal feeding container;
Have door on the sidewall of described casing, described door is used for putting into or taking out laboratory animal or experimental animal feeding container;
Described cooling device contacts with described field generator for magnetic, is used for cooling off described field generator for magnetic;
Described ventilation unit and lighting device are respectively applied to ventilate and illumination for described field generator for magnetic provides; And
Described voltage-operated device is connected with described field generator for magnetic, is used for controlling the magnetic field intensity that described field generator for magnetic produces.
Preferably, described casing is the casing of armoured magnetic field, and described casing is cuboid roughly, and six walls of casing are made by iron plate, and four sidewalls are provided with ventilating opening, and described door is arranged on described four sidewalls sidewall vertical with described field generator for magnetic axis.
Preferably, described field generator for magnetic is solenoid, and described cavity for placing laboratory animal or experimental animal feeding container is described solenoid inner chamber.
Preferably, described detector for magnetic field is arranged in the magnetic field display unit on the front side wall of described casing and is positioned at the magnet field probe of cavity that is used for placing laboratory animal or experimental animal feeding container of described field generator for magnetic.
Preferably, the sidewall of described casing is metal sidewall, is used for armoured magnetic field, stops magnetic field to the interference of staff and control-animal.
Preferably, described temperature detection comprises temperature indicator and the temp probe that is connected with temperature indicator with regulation device.
Preferably, described ventilation unit is exhaust fan, and described exhaust fan is installed on the sidewall of described casing.
Preferably, described lighting device comprises illuminating lamp and luminous lamp switch controller, and described luminous lamp switch controller is used for the described illuminating lamp of loop control to be opened, and realizes the simulation at daytime and night.
Preferably, described cooling device comprises the cooling tube that is wrapped on the described field generator for magnetic, the pump installation that is connected with cooling tube and refrigerant storage container, and described cooling tube, pump installation and refrigerant storage container consist of cooling circuit.
Preferably, the described cavity size be used to breeding experimental animals of described field generator for magnetic can be held standard rat feeding case and/or standard mouse rearing-box, described standard rat feeding case is of a size of (380 ± 20) * (325 ± 20) * (180 ± 20) mm, and described standard mouse rearing-box is of a size of (310 ± 20) * (200 ± 20) * (140 ± 20) mm.
Preferably, described illuminating lamp is the LED lamp.
Preferably, described cooling tube is made by the copper metal, and described cooling agent is water.
Animal model preparation facilities of the present invention can be prepared and can be used in the analysis of magnetic field action to the animal model of the fertility of animals impact.And, this animal model preparation facilities can provide again the magnetic field space that is suitable for laboratory animal existence when enough magnetic field intensity is provided, but also temperature controllable, illumination, circulation of air etc., and can use for a long time, make it possible under the condition near nature, breed experimental animals.In addition, owing to adopted the shielding design, inner AC magnetic field is effectively shielded, guarantee that the experimenter is not subjected to magnetic interference.
Description of drawings
Fig. 1 is the structural representation of an embodiment of the animal model preparation facilities with field generator for magnetic of the present invention;
Fig. 2 is the structural representation of an embodiment of the animal model preparation facilities of Fig. 1;
Fig. 3 is the structural representation of biopsy cavity marker devices of the animal model preparation facilities of Fig. 2, for clarity sake, and the clipped structure;
Fig. 4 has shown the be in heat typical vaginal smear of different phase of rat, and wherein: dioestrus (A) mainly comprises leucocyte and mucus; Proestrum (B) a large amount of karyocyte and cornified vaginal epithelial cells are also deposited; Mainly be cornified vaginal epithelial cell oestrus (C); Oestrus the later stage (D) karyocyte and a large amount of leucocyte and deposit, each vaginal smear all amplifies 100 times of observations at microscopically and takes pictures.
Embodiment
Below with reference to accompanying drawing preferred embodiment of the present invention is elaborated, so that clearer understanding purpose of the present invention, characteristics and advantage.It should be understood that embodiment shown in the drawings is not limitation of the scope of the invention, and just for the connotation of technical solution of the present invention is described.Part identical among the figure uses the same reference numerals to represent.
Fig. 1 is the structural representation with animal model preparation facilities of field generator for magnetic of the present invention.As shown in Figure 1, animal model preparation facilities 100 comprises casing 1, field generator for magnetic 2 (being solenoid), detector for magnetic field (14,18), temperature detection and regulation device (11,12), voltage-operated device 15, cooling device (3,4,5,6), lighting device (9,10) and ventilation unit (7,8).
During work, laboratory animal is raised in the solenoid 2 that is centered on by cooling device 3.The voltage strength of voltage-operated device 15 control solenoids 2, and then the intensity in the magnetic field that produces of control solenoid 2 are to obtain the required magnetic field of experiment.Cooling device for solenoid 2 provides cooling, thereby guarantees that solenoid 2 can work long hours when solenoid is worked or when temperature detection and regulation device detect the solenoid temperature and surpass predetermined value.Lighting device is realized the simulation at daytime and night by switch control, provides closer to the self-sow environment for the laboratory animal of raising in the solenoid 2 with ventilation unit.
Fig. 2 and Fig. 3 illustrate the structural representation of an embodiment of the animal model preparation facilities 100 of Fig. 1.As Figure 1-3, in the present embodiment, casing 1 is cuboid roughly, and six walls of casing 1 are all made by iron plate, wherein, all have louver (or hole) on four sidewalls, are beneficial to circulation of air.On the rear wall of casing (or on other sidewalls) exhaust fan 8 that is connected with controller for air exhaust fan 7 is installed, and is provided with the hole that supplies connecting line and cooling tube 3 (hereinafter with more detailed description) to pass through.Preferably, casing 1 bottom also is provided with the roller (not shown), to make things convenient for mobile box.
Be provided with control and display unit zone 1a and 1b, door-plate 1c at the front side wall of casing 1, can put into or take out the rearing-box 22 that laboratory animal is housed by door 1b, door-plate 1c is also made by iron plate, with armoured magnetic field better.Control and viewing area are provided with power switch 16, current sensing means 17, voltage regulator 15, magnetic field display unit 18, illumination control apparatus 9 and temperature indicator 11.
Wherein, power switch 16 is connected with general supply, voltage regulator 15 and current sensing means 17 are connected to solenoid 2, detector for magnetic field comprises the magnetic field display unit 18 on the front side wall that is arranged in described casing and is positioned at the magnet field probe 14 of solenoid 2 that illumination control apparatus 9 is connected to the illuminating lamp 10 that is arranged in solenoid 2.In addition, although not shown, exhaust fan 8, current sensing means 17, voltage regulator 15, magnetic field display unit 18, illumination control apparatus 9 and temperature indicator 11 etc. all are connected with power supply.
In the above-mentioned animal model preparation facilities 100, size by voltage regulator 15 adjustable voltage, and then the size of adjusting current intensity and the size of magnetic field intensity, simultaneously, the size of current intensity and magnetic field intensity shows in current sensing means 17 and magnetic field display unit 18 respectively in real time, so that the user can observe the variation in electric current and magnetic field easily, thereby magnetic field intensity is adjusted to needed value easily.
Illuminating lamp 10 in the illumination control apparatus 9 automatic cycles control solenoid 2 is with simulate day and night.In the present embodiment, illuminating lamp is the LED lamp, and it is long that it has the life-span, the advantages such as lower calorific value.
Above-mentioned field generator for magnetic is solenoid 2, can according to the magnetic field size that will produce and the needed cultured space of laboratory animal, diameter, coil winding density and the length thereof of solenoid 2 be set.
In the present embodiment, solenoidal diameter (being the diameter of coil) is about 350mm, and solenoid length is about 800mm, the homogeneous area that uniform magnetic field can be provided greater than
The industrial frequency AC magnetic field intensity reaches 0～50.0Gs, and adjustable continuously, and accuracy reaches 2%.
The large I of helix tube inner chamber is held the rat feeding case of two standards, and (size is about the mouse rearing-box of 380 * 325 * 180mm) or 4 standards, and (size is about 310 * 200 * 140mm).But should be understood that solenoidal diameter and length can be set as required, to hold other experimental animal feeding casees, such as the rabbit rearing-box.
In addition, because field generator for magnetic, namely calorific value is large in the course of the work for solenoid 2, and particularly for producing high-intensity magnetic field or the solenoid of the homogeneous area of large uniform magnetic field being provided, calorific value is especially large, so that it can't work long hours.For guaranteeing to work for a long time, be wound with cooling tube 3, cooling tube 3 and solenoid 2 electric insulations at the outer wall of solenoid 2.One end of cooling tube 3 is connected to pump installation 5, and the other end is connected to the container 4 of storage cooling agent, and pump installation 5 other ends also are connected to container 4, consist of thus circulation cooling circuit.Preferably, cooling tube 3 is made by the copper metal, and cooling agent is water.It should be noted that cooling tube 3 also can contact with solenoid 2 by modes such as stickups, as long as can cool off solenoid 2.
By linked switch is set, when solenoid 2 was started working, pump installation 5 was also started working, and so that solenoid 2 is lowered the temperature, thereby guaranteed that solenoid 2 and animal model preparation facilities 100 can work long hours.Perhaps, when temperature detection and regulation device, when namely temp probe 12 detected solenoidal temperature above predetermined value, pump installation started solenoid is lowered the temperature.
The application for preparing animal pattern and prepared animal pattern by animal model preparation facilities of the present invention
1 laboratory animal
Female and the male rat (available from west, Shanghai City pul-Bi Kai laboratory animal Co., Ltd) of healthy SPF level SD.The normal condition of raising is, every day, light application time was 6:00 to 18:00, and animal freely drinks water and ingests, and indoor temperature control is in (20 ± 2) ℃.
2 magnetic fields are in the experimental technique of rat
Get the female rats in 10 4 ages in week, be divided at random 2 groups.5 of experimental group are raised in field generator for magnetic inside, keep magnetic field intensity 1mT, and stimulate 8 hour from 16:00 to 24:00 every day, and continuing stimulated for 4 weeks, for detection of oestrus and blood steroid hormone level.During the magnetic exposure, animal can freely drink water, ingest, and room temperature is controlled at 20 ± 2 ℃.Control group (5) is raised outside the field generator for magnetic of same room 2 meters, the noiseless part in experiment magnetic field.
Similar with above experimental design, experimental group and control group respectively get 8 from 4 ages in week the rats that begin to process, kept for 4 weeks after, mate with 1: 1 ratio with the male mouse of normal adult.Whether next day is by having sperm to determine whether the generation mating in the vaginal smear examination genital tract.The female rats of mating success is raised separately, and the pregnancy duration length of record rat claims to get female mouse and filial generation body weight when dividing 28 days puerperiums.Observe the embryonic development situation, record conceived fate, newborn mouse growth and the young number of nest, with the impact of analysis of magnetic field action on female mouse fecundity.
Get at random 8 of the male rat experimental group in 8 ages in week, keep magnetic field intensity 2mT, every day stimulation time by 18:00 to 06:00, totally 12 hours.Other gets 4 male rats and organizes in contrast.Continue to put to death rat after 60 days, get epididymal sperm, analyze the indexs such as sperm morphology.
The judgement of 3 female rats Each Stage of Estrus Cycles
Get 4 age in week rat, process 4 thoughtful rats through magnetic field and grow up (8 age in week), then begin the vaginal smear examination Estrus Cycle in Rats.Concrete grammar is that morning every day, 8:30 got female mouse to be checked, routinely, got the rat vagina mucus with the round end glass bar of sterilization and was evenly coated on the slide that adds aqua sterilisa, conventional Giemsa staining method dyeing observation.Reference literature is identified the residing Oestrous Stages of rat at microscopically.General plate coating checking continues two more than the cycle.Next, be checked through the morning on the same day in oestrus about 10:30, venous blood sampling 1.2ml under Rat Tongue, separated plasma is for detection of the mensuration of FSH, oestrogenic hormone and progesterone level.
4 hormone test methods
Utilize estradiol and progesterone diagnostic kit (Electrochemiluminescince, German Luo Shi diagnoses Co., Ltd), according to the kit operation instructions, measure E2 and P4 concentration at Luo Shi 2010 electrochemical luminescence immunity analysis instruments.
The analytical method of 5 rat spermatozoas
Rat is got its cauda epididymis after putting to death immediately, fat, blood and tissue juice around removing as far as possible; The cauda epididymidis immigration is contained in the PBS buffer solution (2ml) of 1%BSA.Live a side of cauda epididymis with the fine forceps sub-folder, cut a vertical osculum in other side with eye scissors, the cauda epididymis spermatium is extruded.At 37 ℃, to cultivate 1 hour under the 5%CO2 condition, mixing is drawn 6 μ l suspensions, drips in counting plate for sperm (macro ruby tally, Shanghai little figure instrument development in science and technology Co., Ltd) counting sperm concentration and activity ratio.Count respectively one in the visual field motile sperm and spermatium quantity with the estimation sperm motility, every this counting of increment is total greater than 200 sperms.
Use Transgreen (Shanghai, Shanghai Gan Feng biotech development Co., Ltd) and PI (sigma) to redye and detect the plasmalemmae of sperms integrality.Simply say: with Transgreen and PI mixing, add the fresh spermatozoa suspension, hatch more than the 5min for 37 ℃, get 6 μ l suspensions and drip in counting plate for sperm, in the lower observation of fluorescence microscope (LEICA, DFC360FX).Under the exciting of blue light, the complete sperm of plasma membrane sends green fluorescence, and the injury of plasmalemmae sperm sends red fluorescence.Count respectively red in the same visual field and green sperm quantity, calculate survival rate, every this counting of increment sum surpasses 200 sperms.
6 statistical analysis methods
Observe and detect each index, record relevant data, as variable, process in magnetic field and contrast is recorded as experiment and divides into groups, and inserts in the Excel form with each index.Then relevant data is imported SPSS 18.0 statistical softwares (SPSS Inc., Chicago, IL), according to data type and distribution situation, select t check or Mann-Whitney U check, obtain average and standard deviation, and significant indexes p value.Significance of difference level is selected p＜0.05.
Check magnetic field is on the impact of female rats oestrous cycle and steroid hormone blood levels
Adopt the magnetic field intensity of 1mT, begin to continue 4 weeks of stimulation in rats from 4 ages in week, action time every day is from 16:00 to 24:00.It is restless to find that rat occurred at 2-3 days that put that magnetic field space begins into, recovers gradually normal performance after 3 days.With rat in magnetic field space, raise 4 thoughtful 8 the week ages after, each is organized rat and all begins to occur the oestrous cycle.By the vaginal smear method of routine, check the oestrous cycle of rat.
The result is shown in Fig. 4 and table 1.Fig. 4 has shown the microexamination result of the vaginal smear of oestrous cycle different phase.By the vaginal epithelial cell keratinization situation that comes off wherein, and leucocyte and mucus is how much relative, determines the process in oestrous cycle.Table 1 has recorded from 4 ages in week and has begun magnetic field (1mT) processing to the situation that affects of female rats oestrus and hormonal readiness.
Table 1. begins magnetic field (1mT) processing to the impact of female rats oestrus and hormonal readiness from 4 ages in week
Annotate:
*The p value that relatively obtains by non-parametric test Mann-Whitney U mode between mark expression magnetic field processed group and control group, relatively checking by t of the blood levels of oestrogenic hormone (E2) and progestational hormone (P4) obtains the p value, suppose that variance equates bilateral truncation.
As can be seen from Table 1, the processed group frequency of oestrusing in magnetic field significantly improves, and dioestrus is shorter, and the oestrogenic hormone in oestrus (estradiol) rises to some extent.Yet the blood levels of progestational hormone changes little, without significant difference.
Check magnetic field is on female rats steroid hormone blood levels and reproduction offspring's impact
Process similarly the female age in mouse to 8 week in 4 ages in week.As can be seen from Table 2, the blood levels of oestrogenic hormone and progesterone is not subject to appreciable impact equally, and wherein the p value is all greater than 0.05.In this case, treated female rats and normal bull rat mating, the situation of procreate does not have the difference of statistically significant between processed group and experimental group.
The result is as shown in table 2 below, the young number of nest, the average weight of every nest filial generation, and every nest offspring's sex ratio, magnetic field process and control group between all do not have the difference (p＞0.05) of statistically significant.Female mouse body weight when the pregnancy duration and minute 28 days puerperiums magnetic field process and control group between do not present the difference of statistically significant yet.
Table 2 begins magnetic field (1mT) processing to female rats hormonal readiness and reproduction offspring's impact from 4 ages in week
Note:
*Relatively obtain the p value by the t check between mark expression magnetic field processed group and control group, suppose that variance equates bilateral truncation.Grey marks offspring's index.
8 the week age SD female rats be exposed to the 5mT intensity magnetic field to blood steroid hormone level and reproduction offspring's impact
Get 8 age in week 9 of SD rats, random packet, experimental group are 8, control group is 4.Experimental group places in the magnetic field, and magnetic field intensity is 5mT, and every daily exposure time is 18:00 to 06:00, and light application time is 6:00 to 18:00, and rat freely drinks water and takes food; Control group places outside the magnetic field of same room, and the magnetic field intensity background value is 0.027 μ T.
Table 3 begins magnetic field (5mT) processing to female rats hormonal readiness and reproduction offspring's impact from 8 ages in week
Note:
*Relatively obtain the p value by the t check between mark expression magnetic field processed group and control group, suppose that variance equates bilateral truncation.Grey marks offspring's index.
As shown in table 3.Magnetic exposure continues 60, find that estrogen level reduces in the blood, and progesterone level raises.Surprisingly, with normally with the male mating of strain, the young digital display work of offspring's nest increases (the young number mean value of nest rises to 9.3 from 5.3), this and magnetic field cause the frequency of oestrusing of female rats to rise and have certain correlation, prompting magnetic field has also caused the increase of number of eggs ovulated when frequency rises causing female rodent to oestrus, thereby causes the young number of nest to increase.
Magnetic field is on the impact of male rat fecundity related index
Adopt the electromagnetic field of 2mT intensity to process male adult rat (8 age in week), amount to 12 hour from 18:00 to 06:00 every day, continues 60 days.
The result is as shown in table 4 below, and its testis and epididymis weight and control group do not have statistically significant difference; The membrane integrity of sperm concentration and sperm does not have significant difference yet; But the motile sperm proportion significantly reduces in the magnetic fields group, and the mean value of motile sperm ratio significantly drops to 15.25% from 38.5%.
Table 4 begins magnetic field (2mT) processing to the impact of male rat sperm index from 8 ages in week
Note:
*Relatively obtain the p value by the t check between mark expression magnetic field processed group and control group, suppose that variance equates bilateral truncation.Grey marks p＜0.05, processes in magnetic field and the significant index of contrast difference.
4 the week age SD male rat be exposed to the 2mT/5mT intensity magnetic field to the impact of fecundity related index
Get 4 age in week 15 of SD male rats, random packet, 2mT and 5mT magnetic exposure group respectively are 6, control group is 3.Experimental group places in the magnetic field, and magnetic field intensity is 2mT, and every daily exposure time is 18:00 to 06:00, and light application time is 6:00 to 18:00, and rat freely drinks water and takes food; Control group places outside the magnetic field of same room, and the magnetic field intensity background value is 0.027 μ T.
The result is as shown in table 5.Magnetic exposure continues 60, and the result showed from 4 ages in week, was exposed to the experimental group in 2mT or 5mT magnetic field, and its sperm concentration all reduces with respect to contrast.In addition, be exposed to the experimental group in 2mT or 5mT magnetic field, the shared ratio of its motile sperm all descends, and is more remarkable with the 5mT experimental group.
Table 5 begins magnetic field (2/5mT) processing to the impact of male rat sperm index from 4 ages in week
By above-described embodiment as can be known, can prepare by animal model preparation facilities of the present invention and can be used in the analysis of magnetic field action to the animal model of the fertility of animals impact.And, this animal model preparation facilities can provide again the magnetic field space that is suitable for laboratory animal existence when enough magnetic field intensity is provided, and temperature controllable, illumination, circulation of air etc., and can use for a long time, make it possible under the condition near nature, breed experimental animals.In addition, owing to adopted the shielding design, inner AC magnetic field is effectively shielded, guarantee that the experimenter is not subjected to magnetic interference.
Below described preferred embodiment of the present invention in detail, but it will be appreciated that, after having read above-mentioned instruction content of the present invention, those skilled in the art can make various changes or modifications the present invention.These equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. an animal model preparation facilities comprises casing, field generator for magnetic, detector for magnetic field, temperature detection and regulation device and voltage-operated device, it is characterized in that:
Described preparation facilities also comprises ventilation unit, lighting device and cooling device;
Described field generator for magnetic is arranged in described casing and has for the cavity of placing laboratory animal or experimental animal feeding container;
Have door on the sidewall of described casing, described door is used for putting into or taking out laboratory animal or experimental animal feeding container;
Described cooling device contacts with described field generator for magnetic, is used for cooling off described field generator for magnetic;
Described ventilation unit and lighting device are respectively applied to ventilate and illumination for described field generator for magnetic provides; And
Described voltage-operated device is connected with described field generator for magnetic, is used for controlling the magnetic field intensity that described field generator for magnetic produces.
2. animal model preparation facilities as claimed in claim 1, it is characterized in that: described casing is the casing of armoured magnetic field, described casing is cuboid roughly, six walls of casing are made by iron plate, four sidewalls are provided with ventilating opening, and described door is arranged on described four sidewalls sidewall vertical with described field generator for magnetic axis.
3. animal model preparation facilities as claimed in claim 1, it is characterized in that: described field generator for magnetic is solenoid, described cavity for placing laboratory animal or experimental animal feeding container is described solenoid inner chamber.
4. animal model preparation facilities as claimed in claim 1 is characterized in that: described detector for magnetic field is arranged in the magnetic field display unit on the front side wall of described casing and is positioned at the magnet field probe of cavity that is used for placing laboratory animal or experimental animal feeding container of described field generator for magnetic.
5. animal model preparation facilities as claimed in claim 1, it is characterized in that: described temperature detection comprises temperature indicator and the temp probe that is connected with temperature indicator with regulation device.
6. animal model preparation facilities as claimed in claim 1, it is characterized in that: described ventilation unit is exhaust fan, described exhaust fan is installed on the sidewall of described casing.
7. animal model preparation facilities as claimed in claim 1, it is characterized in that: described lighting device comprises illuminating lamp and luminous lamp switch controller, described luminous lamp switch controller is used for the described illuminating lamp of loop control to be opened, and realizes the simulation at daytime and night.
8. animal model preparation facilities as claimed in claim 1, it is characterized in that: described cooling device comprises the cooling tube that is wrapped on the described field generator for magnetic, the pump installation that is connected with cooling tube and refrigerant storage container, and described cooling tube, pump installation and refrigerant storage container consist of cooling circuit.
9. animal model preparation facilities as claimed in claim 1, it is characterized in that: the described cavity size be used to breeding experimental animals of described field generator for magnetic can be held standard rat feeding case and/or standard mouse rearing-box, described standard rat feeding case is of a size of (380 ± 20) * (325 ± 20) * (180 ± 20) mm, and described standard mouse rearing-box is of a size of (310 ± 20) * (200 ± 20) * (140 ± 20) mm.
10. animal model preparation facilities as claimed in claim 7, it is characterized in that: described illuminating lamp is the LED lamp.
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| CN103734026A (en) * | 2014-01-08 | 2014-04-23 | 上海大学 | Fastener magnetism experiment animal stimulation device |
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Cited By (2)
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| CN103734026A (en) * | 2014-01-08 | 2014-04-23 | 上海大学 | Fastener magnetism experiment animal stimulation device |
| CN103734026B (en) * | 2014-01-08 | 2015-10-07 | 上海大学 | Fastener magnetism experiment animal stimulation device |
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Address after: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee after: Shanghai Institute of biomedical technology Address before: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee before: SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH |