CN2797373Y - Loading device for bone tissue engineering - Google Patents
Loading device for bone tissue engineering Download PDFInfo
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- CN2797373Y CN2797373Y CN 200520026244 CN200520026244U CN2797373Y CN 2797373 Y CN2797373 Y CN 2797373Y CN 200520026244 CN200520026244 CN 200520026244 CN 200520026244 U CN200520026244 U CN 200520026244U CN 2797373 Y CN2797373 Y CN 2797373Y
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- bone tissue
- loading device
- bone
- piezoelectric ceramics
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Abstract
The utility model discloses an incubator which combines direct loading with a perfusion system to improve bone tissue engineering and provides a loading device more suitable to the mechanical environment in the body bone tissue. The loading device of the utility model comprises a planer type rigid frame, a piezoelectric ceramic displacement device, a micrometric displacement capacitive sensor and a culture chamber device, wherein the top of the frame is provided with a pretension regulating screw rod, and the piezoelectric ceramic displacement device is arranged in the frame and is connected with the top; the culture chamber device is arranged in the frame and is connected with the bottom, and the lower part of the piezoelectric ceramic displacement device is provided with the micrometric displacement capacitive sensor. The device is designed for the bone tissue engineering construction, and can be used for other tissue engineering cultures after modification. The utility model has the advantages that the mechanical environment of cultured objects is controlled accurately.
Description
Technical field
The utility model relates to organizational project bio-reactor field, more particularly, is a kind of deceleration loading device that is used for bone tissue engineer.
Background technology
Bio-reactor is the important factor of bone tissue engineer cultivation, and the purpose of bio-reactor is to provide suitable growing condition for the vitro tissue engineering makes up, and makes engineering tissue obtain to biological tissue similar structure and function are arranged.The development of suitable bio-reactor is also depended on the bone structure and the emic degree of understanding except that considering biochemical factor.
Current bio-reactor seldom has at bone tissue engineer and makes up, and normally used have three kinds: i.e. rotating and culturing bottle, rotating wall vessel bioreactor and perfusion type bio-reactor.The bio-reactor of this three classes bone tissue engineer structure has shown all that than static cultivation in the culture dish more advantage is arranged.But the rotating and culturing bottle can not make nutrient solution and other nutrition be penetrated into well in the porous network of timbering material and go, and cell growth and matrix calcification are limited in the outside surface of timbering material.The Object of Development of rotating wall vessel bioreactor can only be isotropic tissue or organ, and support can only be selected the lower material of density.Although the perfusion type bio-reactor allows the hole of nutrient solution by being coupled to each other in the timbering material, the perfusion type bio-reactor can provide better nutrition transmission, and cultured cells is stimulated with shearing stress, but the mechanical environment of perfusion type bio-reactor with still have than big difference at the mechanical environment of body osseous tissue, can't satisfy the cultivation of bone tissue engineerization.
Biomechanics of bone perception conduction mechanism shows the directly dynamically effect of compressive load of normal physiological processes needs of osseous tissue.The bone structure more complicated, the hole of three kinds of different levelss of existence: first kind is the hole that contains blood vessel, and this comprises the hole between bone trabecula, the about 10 μ m of radius; Second kind is the lacuna-bone canaliculus hole that is arranged in matrix osteocyte place, and radius is about 0.1 μ m; The third is the hole of collagen-hydroxylapatite (level), and radius is about 20nm.In the hole of these three kinds of levels, nest falls into-and the bone canaliculus hole is the main lacuna that extraneous load causes liquid-flow, and the osteocyte inner with it constitutes nest to fall into-the bone canaliculus system, is the primary structure of biomechanics of bone perception conduction.The dynamic load effect cause nest fall into-the bone canaliculus hole in liquid-flow, the result causes convection current (convection) transmission of nutrition, refuse.Flowing of liquid not only sent excitation to osteocyte, and guarantees effective transmission of osteocyte signaling molecule, nutrition and refuse.Being arranged in ground substance of bone is not enough by infiltration transmission nutrition and metabolism thing only away from the osteocyte of blood vessel, must fall into by nest-the bone canaliculus hole in liquid to streamed transmission, satisfy the metabolism of osteocyte.From the bone tissue engineer culturing process, perfusion system can apply fluid shearing stress and normal nutrition and refuse transportation to attached cell when complex body was cultivated beginning, but cultivate a stage, scleroblast secretion ground substance of bone, become osteocyte after the embedding, osteocyte is prominent with osteocyte, scleroblast, bone lining cell form cellular network all around by cell, after the ground substance of bone calcification, be nest fall into-after the bone canaliculus hole forms, particularly be formed with certain thickness calcification matrix, only perfusing course is difficult to satisfy the demand of culture this moment.Directly apply dynamic load and just can form similar mechanical environment in the body osseous tissue.
The utility model content
Technical problem to be solved in the utility model is, provides a kind of will directly the loading with perfusion system to combine the incubator that improves bone tissue engineerization, provide can be more approaching at the deceleration loading device of the mechanical environment of body osseous tissue inside.
The utility model is used for the deceleration loading device of bone tissue engineer, be achieved by following technical proposals, deceleration loading device comprises planer-type rigid frame, piezoelectric ceramics shifter, micrometric displacement capacity transducer and cultivates cell equipment, described frame roof is provided with pretension and adjusts screw rod, be provided with the piezoelectric ceramics shifter in the described framework and link with the top, be provided with in the described framework and cultivate cell equipment and link with the bottom, described piezoelectric ceramics shifter bottom is provided with the micrometric displacement capacity transducer.
Cultivation cell one side described in the utility model connects successively by silicone tube cultivates liquid pool, peristaltic pump and cultivates cell opposite side formation filling system.
The timbering material of bone should have certain intensity in the bone tissue engineer cultivation, and suitable mechanical environment must be arranged.The traditional Principle of Mechanical Designing of strain by using that one fritter timbering material reaches the bone physiological level is difficult to accomplish, the utility model adopts intelligent material-piezoelectric ceramics as power resources, can realize that bone holder material bone physiological level strained accurately controls.The strain of different wave (square wave, sine wave, choppy sea) and arbitrarily the waveform strain of hypothesis can realize that all this provides condition for through engineering approaches makes up the unified research of the structure of osseous tissue and function.This Design of device is the research for load influence in the through engineering approaches osseous tissue structure, simultaneously through becoming the bio-reactor of good in-vitro bone tissue engineer cultivation after improving.
Adopt the piezoelectric ceramics shifter as the deceleration loading device power resources, this is the most important advantage of this device.The piezoelectric ceramics shifter has compact construction, volume is little, nothing rubs, drive the characteristics sensitive, that the micrometric displacement precision is high; In addition, use the piezoelectric ceramics shifter, because the speed that electromechanical Coupling is carried out is very fast, there is not heating problem, the electricity that influences culturing cell is led with thermal radiation and can be ignored, because the piezoelectric ceramics shifter similar structure that is electrical condenser, thus minimum power consumption is arranged, even at a high speed and also be like this under the high voltage drive situation.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is that the utility model is cultivated the cell structure synoptic diagram;
Fig. 3 is the utility model perfusion system flow circuit synoptic diagram.
Embodiment
Below in conjunction with accompanying drawing the utility model is described further.
As shown in Figure 1, deceleration loading device comprises planer-type rigid frame 1, piezoelectric ceramics shifter 2, micrometric displacement capacity transducer 3 and cultivates cell 4, frame roof is provided with pretension and adjusts screw rod 5, be provided with piezoelectric ceramics shifter 2 in the framework and contact with the top, be provided with in the framework and cultivate cell 4 and touch with bottom connection, piezoelectric ceramics shifter 2 bottoms are provided with micrometric displacement capacity transducer 3.
Wherein cultivate cell structure as shown in Figure 2, above the cell lid 9 cell is set and covers briquetting 8; Cell covers 9 bottoms and is provided with lower lock block 10.
Adopt intelligent material-piezoelectric ceramics shifter (WTYD081045 type) as core component, connect and compose the loading power system, be the osseous tissue cultivation by Microcomputerized with piezoelectric ceramics power supply
The load (square wave, sine wave, choppy sea etc.) of different wave in the physiological range is provided.Has the output accuracy height, characteristics simple for structure.
As shown in Figure 3, cultivating cell 4 one sides connects cultivation liquid pool 6, peristaltic pump 7 successively and cultivates cell 4 opposite sides formation filling system by silicone tube.By the driving of peristaltic pump, nutrient solution is pumped out from cultivating liquid pool, flows through the cultivation cell then, flows back to the cultivation liquid pool again.The circumfusion subsystem can guarantee effective transportation of culture nutrition, refuse, has controlled perfusion rate simultaneously, for culture provides variable mobile shearing stress.
Although this device designs at the bone tissue engineer structure, the mechanical environment of cartilage tissue engineeredization structure also can be provided, perhaps also can be used for other engineered cultivation through improving, be characterized in that the mechanical environment of culture is accurately controlled.This Design of device is improved the bio-reactor that can become the cultivation of a good in-vitro bone tissue engineer, for example, adopt grating instrument Displacement Measurement, be directly changed into strain unit, and realize feedback control by connecting microcomputer, make the complex body strain of cultivation dynamically be controlled at certain physiology range of strain, this meets at body osseous tissue strained Changing Pattern.
Claims (2)
1. deceleration loading device that is used for bone tissue engineer, it is characterized in that, described deceleration loading device comprises planer-type rigid frame, piezoelectric ceramics shifter, micro-displacement sensor and cultivates cell equipment, described frame roof is provided with pretension and adjusts screw rod, be provided with the piezoelectric ceramics shifter in the described framework and link with the top, be provided with in the described framework and cultivate cell equipment and link with the bottom, described piezoelectric ceramics shifter bottom is provided with micro-displacement sensor.
2. the deceleration loading device that is used for bone tissue engineer according to claim 1 is characterized in that, described cultivation cell one side connects successively by silicone tube cultivates liquid pool, peristaltic pump and cultivate cell opposite side formation filling system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520026244 CN2797373Y (en) | 2005-06-21 | 2005-06-21 | Loading device for bone tissue engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520026244 CN2797373Y (en) | 2005-06-21 | 2005-06-21 | Loading device for bone tissue engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2797373Y true CN2797373Y (en) | 2006-07-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520026244 Expired - Fee Related CN2797373Y (en) | 2005-06-21 | 2005-06-21 | Loading device for bone tissue engineering |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101397539B (en) * | 2008-10-14 | 2011-10-05 | 中国人民解放军第三军医大学 | Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering |
| CN102559493A (en) * | 2012-02-15 | 2012-07-11 | 四川大学 | Microvibration loading device for bone formation related cells |
| CN111057640A (en) * | 2019-12-09 | 2020-04-24 | 西北工业大学 | Experimental device for in-situ research cell mechanical characteristics |
-
2005
- 2005-06-21 CN CN 200520026244 patent/CN2797373Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101397539B (en) * | 2008-10-14 | 2011-10-05 | 中国人民解放军第三军医大学 | Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering |
| CN102559493A (en) * | 2012-02-15 | 2012-07-11 | 四川大学 | Microvibration loading device for bone formation related cells |
| CN111057640A (en) * | 2019-12-09 | 2020-04-24 | 西北工业大学 | Experimental device for in-situ research cell mechanical characteristics |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060719 Termination date: 20100621 |