CN114672416A - Tumor cell culture device - Google Patents
Tumor cell culture device Download PDFInfo
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- CN114672416A CN114672416A CN202210373927.4A CN202210373927A CN114672416A CN 114672416 A CN114672416 A CN 114672416A CN 202210373927 A CN202210373927 A CN 202210373927A CN 114672416 A CN114672416 A CN 114672416A
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- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 34
- 238000004113 cell culture Methods 0.000 title claims abstract description 31
- 230000005540 biological transmission Effects 0.000 claims abstract description 46
- 238000010438 heat treatment Methods 0.000 claims abstract description 24
- 238000000429 assembly Methods 0.000 claims abstract description 6
- 230000000712 assembly Effects 0.000 claims abstract description 6
- 238000009434 installation Methods 0.000 claims description 31
- 230000000694 effects Effects 0.000 claims description 15
- 239000011111 cardboard Substances 0.000 claims description 12
- 230000000149 penetrating effect Effects 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 12
- 230000010354 integration Effects 0.000 claims description 5
- 244000309464 bull Species 0.000 claims 9
- 210000004027 cell Anatomy 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention provides a tumor cell culture device, which relates to the technical field of medical instruments and comprises a device shell, wherein a culture groove is formed in the device shell, a plurality of heating plates are arranged at the center of the interior of the culture groove, transmission assemblies are arranged in the culture grooves on two sides of the heating plates, and a limiting sliding groove is formed in one side of the inner wall of the culture groove .
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a tumor cell culture device.
Background
In order to better understand the tumor cells, in the prior art, a culture device is needed to be used to perform the culture operation on the required cells, and further, the operation such as observation and recording is convenient for the personnel, for example, a tumor cell culture device for anti-tumor experiments is proposed in the patent with application number CN202020777069.6, which comprises an incubator, a sealed cabin door is hinged at the front end of the incubator, the incubator comprises a housing, and a controller for controlling the temperature is assembled at the top end of the housing, the utility model provides an incubator with a culture rack, when in use, a culture dish is placed on the outer surface of a fixed rod and a sleeve piece inside the culture rack, then the cabin door is closed and enters the culture state, in the process of culture, in order to ensure the constant temperature inside the incubator, a temperature sensor assembled on the lower surface of a connecting rod detects the temperature of each culture dish placed near the top of the culture dish, the controller detects its temperature numerical value according to corresponding temperature afterwards, starts corresponding fan for the tuyere that corresponds the below blows off hot-blastly, guarantees near the constant temperature effect of culture dish, and effectual current incubator of having solved adopts the inside bulk temperature of box to control, therefore the constant temperature precision is general, the not good problem of temperature control effect.
However, the above culture apparatus has the following disadvantages in use: the culture dish is required to be used for storing and culturing cells when the cells are cultured, wherein the culture dish is easy to damage when the culture dish is taken due to the fact that the hardness of the common material of the culture dish is low, and the device is not uniform enough in internal heating when the internal heating is carried out, so that the temperature inside part of the culture dish is low, and the cells inside the culture dish cannot be cultured smoothly.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a tumor cell culture device, which solves the problems that the culture dish is easy to damage when the device is used for taking the culture dish due to the lower hardness of the common culture dish material, and the internal heating is not uniform enough when the internal heating is carried out, so that the internal temperature of part of the culture dish is lower, and the internal cells can not be cultured smoothly.
(II) technical scheme
In order to achieve the purpose, the invention adopts the technical scheme that:
a tumor cell culture device comprises a device shell, wherein a culture tank is arranged in the device shell, a plurality of heating plates are arranged in the center of the interior of the culture tank, transmission assemblies are arranged in the culture tank and positioned on two sides of the heating plates, a limiting sliding groove is arranged on one side of the inner wall of the culture tank, a placing assembly is arranged between the limiting sliding groove and the transmission assemblies, and two outer connecting pipes are respectively communicated with one side of the upper surface of the device shell in a penetrating manner;
the placing component comprises a transmission belt, a plurality of arc-shaped expansion plates are integrally arranged on the outer surface of the transmission belt, and a vessel placing plate for fixing a culture vessel is arranged in each arc-shaped expansion plate through a rotating mounting rod;
the device is characterized in that a through hole penetrates through the middle of the upper surface of the device shell, a ventilation assembly is movably mounted inside the through hole, a transparent baffle is integrally arranged on one side of the upper surface of the device shell, a limiting clamping groove is integrally formed in the middle of a transparent baffle plate body, a vessel through groove is formed in the transparent baffle plate body and the limiting clamping groove in a penetrating mode, and the vessel through groove is communicated with the inside of the culture groove.
Preferably, a rotating through hole is formed in the middle of each arc-shaped expansion plate in a penetrating mode, a rotating installation rod is arranged in each rotating through hole in a penetrating mode, and a vessel placing plate is integrally arranged at the top end of the rod body of each rotating installation rod.
As preferred, every arc expansion board outside middle part has all been seted up spacing slot of wearing, every spacing slot of wearing inside equal spacing interlude is provided with spacing picture peg of wearing, every spacing picture peg outer end equal fixed mounting of wearing has the rotation installation pole, every it all is provided with spacing slide bar, every to rotate installation pole tip spacing slide bar equal activity is spacing to be set up inside spacing spout.
As preferred, every the household utensils are placed board upper surface both sides fixed mounting respectively has side position installation piece, and every two are adjacent side position installation piece opposition surface has all been seted up movable spacing groove, every the equal fixed mounting of activity spacing inslot wall has supporting spring, every the equal movable spacing of activity spacing inslot portion has the extension bracing piece, every extend the bracing piece all with correspond to support between the supporting spring and support the tight cooperation, keep away from every of supporting spring one side the equal fixed mounting in extension bracing piece surface has the arc cardboard, every the inboard homogeneous body of arc cardboard is provided with the guiding groove.
Preferably, the lower end rod body of each rotary mounting rod is uniformly provided with a plurality of matching teeth, matching toothed plates are fixedly mounted at the bottoms of the culture tanks on two sides of the transmission assembly respectively, and each matching toothed plate is in transmission matching with the corresponding matching teeth.
Preferably, ventilation assembly is including the subassembly mainboard, integration is provided with the side position draw-in groove respectively in subassembly mainboard body middle part both sides, is located side position draw-in groove one side fixed surface installs the control handle on the subassembly mainboard body, the activity of control handle is inside running through the through-hole.
Preferably, every side position draw-in groove slides the card respectively and goes into the through-hole inner wall department that runs through that corresponds, subassembly mainboard board body is kept away from side position draw-in groove one side and is run through and seted up a plurality of through-holes of taking a breath.
Preferably, the transmission assembly comprises a first rotating rod and a second rotating rod, the first rotating rod and the second rotating rod are respectively rotatably installed in the culture tank and located on two sides of the heating plate, installation shafts are fixedly installed on one sides of the rod bodies of the first rotating rod and the second rotating rod, and the installation shafts are in transmission connection through a transmission belt.
As preferred, be close to first rotor one side culture tank bottom fixed mounting has the motor, motor output fixed mounting has the driving disc, is close to driving disc one side first rotor pole body has seted up the cooperation and has rotated the groove, carry out the transmission cooperation between cooperation rotation groove and the driving disc, first rotor pole body runs through device casing top fixed mounting has the control carousel.
As preferred, be located household utensils and lead to groove one side culture trough bottom fixed mounting has electric push rod, electric push rod's output fixed mounting has a dish, the cooperation of laminating is rotated the installation pole with corresponding to the top dish, the household utensils are placed the board and are led to the inslot wall with household utensils and be spacing card and.
Compared with the prior art, the invention has the following beneficial effects:
1. can carry out stable storage with the household utensils of a plurality of required cultivations through placing the subassembly, and can also cooperate with drive assembly, make this device user's operation of can facilitating more, effectively solved, because of the general material hardness of culture dish is lower, lead to appearing the problem that the culture dish damaged easily when the culture dish is taken at above-mentioned device, reduce the probability that the accident appears in the operation, and then strengthen the security when cultivateing tumor cells, wherein in order to make this subassembly can stably carry out continuous displacement, it is provided with spacing picture peg to wear through the inside equal spacing interlude of every spacing slot of wearing, thereby make every spacing slide bar can move about spacing setting inside spacing spout, avoid appearing because of its distance receives the problem of restriction when the corner appearing, and then ensure that this device can normally function.
2. Carry out the external tight operation of supporting through supporting spring to extending the bracing piece, and then make the extension bracing piece can carry out spacing operation to the arc cardboard, make two arc cardboards can carry out the position to the household utensils of placing and prescribe a limit to, and then make this device can realize stable arrangement have a plurality of household utensils to avoid taking place the problem appearance that drops in placing, wherein through the arc cardboard, and then be convenient for operating personnel can put into fast or take out the culture dish, it is more swift when making this device use, through when using two outer pipes respectively with external carbon dioxide pipeline, the humidification pipeline is connected, the cooperation heating plate realizes keeping its inside constant temperature and humidity constant gas environment under at later stage culture's in-process, make the cultivation effect promote.
3. In order to facilitate taking-out operation of an operator, the required vessel is adjusted to the vessel through groove, the electric push rod pushes the rotary mounting rod, and the vessel placing plate and the inner wall of the vessel through groove are in a limiting clamp shape and can be taken out from the limiting clamp groove; carry out transmission fit through every cooperation pinion rack all with between corresponding cooperation tooth, thereby make when carrying out the position removal make every rotation installation pole carry out the rotation, and then make every household utensils place the board and rotate thereupon, make this subassembly can also carry out the adjustment of angle to every culture dish under the circumstances of carrying out a plurality of position removal, make every face of culture dish can both heat and target in place, and then effectively solved and carried out when the internal heating not enough evenly of its internal heating, thereby lead to the inside temperature of part culture dish lower and then make the problem that inside cell can not be cultivateed smoothly.
4. Can carry out the transmission through the position that the subassembly was placed to the transmission subassembly and handle, and can be the manual control of motor automatic transmission or control carousel when the transmission, wherein automatic rotation is adapted to in cultivateing in order to realize carrying out the transmission that the comprehensive heating was gone on to inside, the manual control of control carousel is adapted to and selects the adjustment operation when the user takes out the household utensils of its inside storage, and then the operating personnel of being convenient for cooperation transparent baffle takes out required household utensils and also can be convenient for the operating personnel adjustment and place the position.
Drawings
FIG. 1 is a schematic view of the overall structure of a tumor cell culture apparatus according to the present invention;
FIG. 2 is a schematic side view of a tumor cell culture apparatus according to the present invention;
FIG. 3 is a schematic top view of a tumor cell culture apparatus according to the present invention;
FIG. 4 is a schematic cross-sectional view taken along line A-A in FIG. 3 of a tumor cell culture apparatus according to the present invention;
FIG. 5 is a schematic view of the internal structure of a tumor cell culture apparatus according to the present invention;
FIG. 6 is a schematic diagram of the structure of the placement part of a tumor cell culture apparatus according to the present invention;
FIG. 7 is a schematic diagram of a top view of a tumor cell culture apparatus according to the present invention;
FIG. 8 is a schematic cross-sectional view taken along line B-B in FIG. 7 of a tumor cell culture apparatus according to the present invention;
FIG. 9 is a schematic diagram of a frame structure of a tumor cell culture apparatus according to the present invention;
FIG. 10 is a schematic front view of a frame structure of a tumor cell culture apparatus according to the present invention;
FIG. 11 is a schematic cross-sectional view taken along line C-C in FIG. 10 of a tumor cell culture apparatus according to the present invention;
FIG. 12 is a schematic diagram of the structure D-D in FIG. 10 of a tumor cell culture apparatus according to the present invention.
In the figure: 1. a device housing; 101. a culture tank; 102. a through hole is penetrated; 2. a transmission assembly; 201. a control dial; 202. a first rotating lever; 203. a matching rotation groove; 204. a second rotating rod; 205. installing a shaft; 3. a limiting clamping groove; 4. a ventilation assembly; 401. a component main board; 402. a side position clamping groove; 403. a control grip; 404. a ventilation through hole; 5. a transparent baffle; 6. a vessel through groove; 7. placing the component; 701. a ware placement plate; 7011. a side position mounting block; 7012. an arc-shaped clamping plate; 7013. extending the support rod; 7014. a movable limiting groove; 7015. a support spring; 7016. a guide groove; 702. a transmission belt; 7021. an arc-shaped expansion plate; 7022. a limiting slide bar; 7023. rotating the mounting rod; 7024. matching teeth; 7025. a limiting through inserting plate; 7026. limiting the inserting slot; 7027. rotating the through hole; 8. heating plates; 9. an electric push rod; 10. a top tray; 11. a motor; 12. a drive plate; 13. a limiting chute; 14. a toothed plate is matched; 15. and (4) an external connecting pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
As shown in fig. 1 to 12, a tumor cell culture device comprises a device housing 1, wherein a culture tank 101 is arranged inside the device housing 1, a plurality of heating plates 8 are arranged at the center inside the culture tank 101, transmission assemblies 2 are arranged inside the culture tank 101 at two sides of the heating plates 8, a limiting sliding groove 13 is arranged at one side of the inner wall of the culture tank 101, a placing assembly 7 is arranged between the limiting sliding groove 13 and the transmission assemblies 2, and two external connecting pipes 15 are respectively communicated with one side of the upper surface of the device housing 1 in a penetrating manner;
the placing component 7 comprises a transmission belt 702, a plurality of arc-shaped expansion plates 7021 are integrally arranged on the outer surface of the transmission belt 702, and a vessel placing plate 701 for fixing a culture vessel is arranged inside each arc-shaped expansion plate 7021 through a rotating mounting rod 7023;
the middle part of the upper surface of the device shell 1 is provided with a through hole 102 in a penetrating manner, a ventilation assembly 4 is movably arranged in the through hole 102 in a penetrating manner, a transparent baffle 5 is integrally arranged on one side of the upper surface of the device shell 1, a limiting clamping groove 3 is integrally arranged in the middle part of a plate body of the transparent baffle 5, a vessel through groove 6 is formed in the transparent baffle 5 and the limiting clamping groove 3 in a penetrating manner, and the vessel through groove 6 is communicated with the culture tank 101 in a penetrating manner.
In this embodiment, every arc expansion board 7021 middle part is run through and has been seted up rotation through-hole 7027, every rotates that through-hole 7027 is inside all to run through and is provided with rotation installation pole 7023, every rotates installation pole 7023 pole body top integration and is provided with household utensils and place board 701, spacing slot 7026 of wearing has all been seted up at every arc expansion board 7021 outside middle part, the inside equal spacing interlude of every spacing slot 7026 of wearing is provided with spacing interlude board 7025, the equal fixed mounting in every spacing picture peg 7025 outer end of wearing has rotation installation pole 7023, every rotates installation pole 7023 tip and all is provided with spacing slide bar 7022, every spacing slide bar 7022 all moves about spacing setting inside spacing spout 13. Can stabilize the storage with the household utensils of a plurality of required cultivations through placing subassembly 7, and can also cooperate with drive assembly 2, make this device can be convenient for user's operation more, effectively solved, it is lower because of the general material hardness of culture dish, lead to appearing the problem that the culture dish damaged easily when the culture dish is taken at above-mentioned device and appear, reduce the probability that the accident appears in the operation, and then strengthen the security when cultivateing tumor cells, wherein can stably carry out continuous displacement in order to make this subassembly, it is provided with spacing interlude board 7025 to wear inside equal spacing interlude of slot 7026 through every spacing, thereby make every spacing slide bar 7022 can the activity spacing setting inside spacing spout 13, avoid appearing because of the problem that its distance received the restriction when the corner appears, and then ensure that this device can normally operate.
What need explain, every household utensils are placed board 701 upper surface both sides fixed mounting respectively has side position installation piece 7011, activity spacing groove 7014 has all been seted up to per two adjacent side position installation pieces 7011 opposite surfaces, the equal fixed mounting of every activity spacing groove 7014 inner wall has supporting spring 7015, the inside equal movable spacing of every activity spacing groove 7014 is installed and is extended bracing piece 7013, every is extended bracing piece 7013 all with correspond to support spring 7015 between support spring 7015 and support the cooperation of supporting tightly, keep away from every extension bracing piece 7013 surface of supporting spring 7015 one side and all fixed mounting have arc cardboard 7012, the inboard homogeneous of every arc cardboard 7012 is provided with guiding groove 7016. Support the tight operation to extending bracing piece 7013 externally through supporting spring 7015, and then make to extend bracing piece 7013 and can carry out spacing operation to arc cardboard 7012, make two arc cardboards 7012 can carry out the position to the household utensils of placing and prescribe a limit to, and then make this device can realize stable arrangement have a plurality of household utensils to avoid taking place the problem appearance that drops in placing, wherein through arc cardboard 7012, and then the operating personnel of being convenient for can put into fast or take out the culture dish, make this device more swift during the use.
When the device is specifically arranged, the lower end rod body of each rotating installation rod 7023 is provided with a plurality of matching teeth 7024 in an integrated manner, the bottoms of the culture tanks 101 at the two sides of the transmission assembly 2 are respectively and fixedly provided with matching toothed plates 14, and each matching toothed plate 14 is in transmission matching with the corresponding matching teeth 7024. Carry out the transmission cooperation through every cooperation pinion rack 14 all with between the corresponding cooperation tooth 7024, thereby make when carrying out the position removal make every rotate installation pole 7023 carry out the rotation, and then make every household utensils place board 701 and rotate thereupon, make this subassembly can also carry out the adjustment of angle to every culture dish under the condition that goes on moving a plurality of positions, make every face of culture dish can both heat and target in place, and then it is not enough even to have effectively solved when carrying out the internal heating, thereby lead to the inside temperature of part culture dish lower and then make the problem that inside cell can not cultivate smoothly.
It can be understood that in this application, ventilation assembly 4 is including subassembly mainboard 401, subassembly mainboard 401 board body middle part both sides respectively the integration be provided with side position draw-in groove 402, the fixed surface installs control handle 403 on the subassembly mainboard 401 board body that is located side position draw-in groove 402 one side, the activity of control handle 403 is inside running through-hole 102, every side position draw-in groove 402 slides the card respectively and goes into the through-hole 102 inner wall department that runs through in the correspondence, subassembly mainboard 401 board body is kept away from side position draw-in groove 402 one side and is run through and seted up a plurality of through-holes 404 of taking a breath. Make this device can manually adjust the inside ventilation flow of culture tank 101 as required through ventilation subassembly 4, and then make this device can realize multiple cultivation environment, move subassembly mainboard 401 through control handle 403 when using ventilation subassembly 4, wherein slide respectively through every side position draw-in groove 402 and block in the through hole 102 inner wall department that runs through that corresponds for control the quantity that ventilation through hole 404 exposes.
It should be noted that the transmission assembly 2 includes a first rotating rod 202 and a second rotating rod 204, the first rotating rod 202 and the second rotating rod 204 are respectively installed inside the culture tank 101 in a rotating manner and located at two sides of the heating plate 8, an installation shaft 205 is fixedly installed at one side of the first rotating rod 202 and one side of the second rotating rod 204, the two installation shafts 205 are connected in a transmission manner through a transmission belt 702, a motor 11 is fixedly installed at the bottom of the culture tank 101 near one side of the first rotating rod 202, a transmission disc 12 is fixedly installed at an output end of the motor 11, a matching rotating groove 203 is formed at one side of the first rotating rod 202 near the transmission disc 12, the matching rotating groove 203 is in transmission matching with the transmission disc 12, a control rotating disc 201 is fixedly installed at the top of the first rotating rod 202 penetrating through the device housing 1, the position where the assembly 7 is placed can be subjected to transmission processing through the transmission assembly 2, and during transmission, the automatic transmission of the motor 11 or the manual control of the control rotating disc 201 can be performed, wherein automatic rotation is adapted to carrying out the transmission that carries out comprehensive heating to inside in cultivateing in order to realize, and the manual control of control carousel 201 is adapted to and selects the adjustment operation when the user takes out the household utensils of its inside storage, and then the operating personnel of being convenient for cooperates transparent baffle 5 to take out required household utensils and also can be convenient for operating personnel to adjust and place the position.
Wherein, be located the culture tank 101 bottom fixed mounting that the groove 6 one side was led to the household utensils and have electric push rod 9, electric push rod 9's output fixed mounting has a dish 10, and a dish 10 with correspond rotate the installation pole 7023 and laminate the cooperation, the household utensils are placed board 701 and the through groove 6 inner wall of household utensils and are spacing card sum. Wherein in order to facilitate operating personnel to take out the operation, through adjusting required household utensils to the logical groove of household utensils 6 after, wherein electric push rod 9 promotes and rotates installation pole 7023, and then makes the household utensils place board 701 and the logical groove of household utensils 6 inner wall for spacing card with, take out from spacing draw-in groove 3 can.
The working principle of the tumor cell culture device is as follows:
when in use, firstly, the two external connecting pipes 15 are respectively connected with the external carbon dioxide pipeline and the humidifying pipeline, so as to keep the internal part of the external connecting pipes to be in a constant humidity and constant gas environment in the later culture process, the control turntable 201 utilizes the transparent baffle 5 to observe whether the vessel placing plate 701 is required to be stored or not, after the observation is finished, the electric push rod 9 is started to lift the current vessel placing plate 701, then the required vessel is placed in the vessel placing plate 701, the electric push rod 9 is reset after the placement, then the motor 11 is started to drive the placing assembly 7 to rotate around the heating plate 8, wherein the matching toothed plate 14 can be matched with the matching teeth 7024 to realize the continuous angle adjustment of the vessel placing plates 701, the uniform heating operation of the vessels on the vessel placing plates 701 is realized, and when the vessels are taken out, the control turntable 201 is controlled to be adjusted to the required vessel, then, the electric push rod 9 is started to lift the current utensil, and the utensil can be taken out through the limiting clamping groove 3.
It should be noted that the specific model specification of the heating plate 8 is FC-004;
the specific model specification of the electric push rod 9 is HTA 2500;
the specific model specification of the motor 11 is MR-42 ZY.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A tumor cell culture device comprising a device housing (1), characterized in that: a culture tank (101) is arranged in the device shell (1), a plurality of heating plates (8) are arranged in the center of the interior of the culture tank (101), transmission assemblies (2) are arranged in the culture tank (101) positioned on two sides of each heating plate (8), a limiting sliding groove (13) is arranged on one side of the inner wall of the culture tank (101), a placing assembly (7) is arranged between each limiting sliding groove (13) and each transmission assembly (2), and two outer connecting pipes (15) are respectively communicated with one side of the upper surface of the device shell (1) in a penetrating manner;
the placing component (7) comprises a transmission belt (702), a plurality of arc-shaped expansion plates (7021) are integrally arranged on the outer surface of the transmission belt (702), and a vessel placing plate (701) for fixing a culture vessel is arranged in each arc-shaped expansion plate (7021) through a rotary mounting rod (7023);
the device is characterized in that a through hole (102) penetrates through the middle of the upper surface of the device shell (1), a ventilation assembly (4) is movably mounted inside the through hole (102), a transparent baffle (5) is integrally arranged on one side of the upper surface of the device shell (1), a limiting clamping groove (3) is integrally formed in the middle of the transparent baffle (5) and the plate body, a vessel through groove (6) is formed in the transparent baffle (5) and the limiting clamping groove (3) in an internal penetrating mode, and the vessel through groove (6) is communicated with the culture tank (101) in an internal mode.
2. A tumor cell culture apparatus according to claim 1, wherein: every arc expansion board (7021) middle part is run through and is seted up rotation through-hole (7027), every it all runs through and is provided with rotation installation pole (7023) to rotate through-hole (7027) inside, every it is provided with household utensils and places board (701) to rotate installation pole (7023) pole body top integration.
3. A tumor cell culture apparatus according to claim 2, wherein: every spacing slot (7026), every of wearing have all been seted up at arc expansion board (7021) outside middle part spacing slot (7026) of wearing is inside all spacing interlude be provided with spacing picture peg (7025), every spacing picture peg (7025) outer end equal fixed mounting is worn to rotate installation pole (7023), every it all is provided with spacing slide bar (7022) to rotate installation pole (7023) tip, every spacing slide bar (7022) equal activity is spacing to be set up inside spacing spout (13).
4. A tumor cell culture apparatus according to claim 2, wherein: every household utensils are placed board (701) upper surface both sides fixed mounting respectively has side position installation piece (7011), and every two are adjacent side position installation piece (7011) opposite surface has all seted up movable spacing groove (7014), every the equal fixed mounting of activity spacing groove (7014) inner wall has supporting spring (7015), every the inside equal movable spacing of activity spacing groove (7014) is installed and is extended bracing piece (7013), every extend bracing piece (7013) and correspond and support between spring (7015) support to the tight cooperation, keep away from every of supporting spring (7015) one side extend bracing piece (7013) equal fixed mounting in surface has arc cardboard (7012), every the inboard homogeneous of arc cardboard (7012) is provided with guiding groove (7016).
5. A tumor cell culture apparatus according to claim 2, wherein: every rotate installation pole (7023) lower extreme pole body unification and be provided with a plurality of cooperation teeth (7024), be located transmission assembly (2) both sides culture tank (101) bottom respectively fixed mounting have cooperation pinion rack (14), every cooperation pinion rack (14) all with correspond carry out the transmission cooperation between cooperation tooth (7024).
6. A tumor cell culture apparatus according to claim 1, wherein: ventilation subassembly (4) is including subassembly mainboard (401), subassembly mainboard (401) body middle part both sides integration respectively are provided with side position draw-in groove (402), are located side position draw-in groove (402) one side surface fixed mounting has control handle (403) on subassembly mainboard (401) body, control handle (403) activity is inside running through-hole (102).
7. The tumor cell culture apparatus according to claim 6, wherein: each side position draw-in groove (402) slides respectively and blocks in corresponding through hole (102) inner wall department, subassembly mainboard (401) board body is kept away from side position draw-in groove (402) one side and is run through and seted up a plurality of through holes of taking a breath (404).
8. A tumor cell culture apparatus according to claim 1, wherein: drive assembly (2) are including first bull stick (202) and second bull stick (204), first bull stick (202) and second bull stick (204) rotate respectively and install in culture tank (101) inside and be located the both sides of hot plate (8), first bull stick (202) and second bull stick (204) pole body one side fixed mounting has installation axle (205), two carry out the transmission through drive belt (702) between installation axle (205) and connect.
9. The tumor cell culture apparatus of claim 8, wherein: be close to first bull stick (202) one side culture trough (101) bottom fixed mounting has motor (11), motor (11) output end fixed mounting has drive plate (12), is close to drive plate (12) one side cooperation rotation groove (203) have been seted up to first bull stick (202) pole body, carry out the transmission cooperation between cooperation rotation groove (203) and drive plate (12), first bull stick (202) pole body runs through device casing (1) top fixed mounting and has control carousel (201).
10. A tumor cell culture apparatus according to claim 5, wherein: be located that the household utensils lead to groove (6) one side culture trough (101) bottom fixed mounting has electric push rod (9), the output end fixed mounting of electric push rod (9) has top dish (10), top dish (10) rotate with corresponding and install pole (7023) and laminate the cooperation, the household utensils are placed board (701) and are led to groove (6) inner wall with the household utensils and be spacing card and.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210373927.4A CN114672416A (en) | 2022-04-11 | 2022-04-11 | Tumor cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210373927.4A CN114672416A (en) | 2022-04-11 | 2022-04-11 | Tumor cell culture device |
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| CN202210373927.4A Pending CN114672416A (en) | 2022-04-11 | 2022-04-11 | Tumor cell culture device |
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