CN2616547Y - Vertical photosynthetic bacterial culture apparatus - Google Patents

Vertical photosynthetic bacterial culture apparatus Download PDF

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Publication number
CN2616547Y
CN2616547Y CNU032209045U CN03220904U CN2616547Y CN 2616547 Y CN2616547 Y CN 2616547Y CN U032209045 U CNU032209045 U CN U032209045U CN 03220904 U CN03220904 U CN 03220904U CN 2616547 Y CN2616547 Y CN 2616547Y
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cylinder body
vertical
screw rod
agitator
cylinder
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Expired - Fee Related
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CNU032209045U
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Chinese (zh)
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黄海智
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a vertical photosynthetic bacterial culturing device which is mainly composed of a cylinder body (8), a cylinder cover (3), a support bracket (10), a screw rod (7), a wall brush (6), an agitator (9), a handle (24), etc., wherein the cylinder body (8) and the cylinder cover (3) are made of transparent material of pressing resistance and are connected by bolts, the cylinder body (8) is made into a vertical regular cuboid shape or a vertical long round barrel shape, and the bottom part of the cylinder body (8) is provided with a material placing opening (27) which can be connected with a material placing valve (18). When the handle (24) can be rotated, the screw rod (7) drives the agitator (9) to rotate and the wall brush (6) to vertically move in order to play the functions of agitating culture fluid and cleaning the attachment on the inner wall of the cylinder body (8). The support bracket (10) is provided with a lamp rack (16) which can be provided with an electric lamp (15) for providing artificial illumination. The utility model has the advantages of convenient manufacture, less investment, convenient use and high productive efficiency, and is suitable for large scale production.

Description

Vertical photosynthetic bacterium culture apparatus
The utility model relates to a kind of production unit of biotechnological formulation, belongs to photosynthetic anaerobion culture apparatus.
Photosynthetic bacterium be a kind of can be in dark the prokaryotic organism of growth and breeding under the aerobic or illumination anaerobic condition, and growth and breeding speed is far longer than dark aerobic condition under the illumination anaerobic condition, so the industrial mass production photosynthetic bacterium mainly is to carry out under the illumination anaerobic condition.Photosynthetic bacterium has the attached wall phenomenon of the light of becoming in culturing process, often stir to keep transparence.Industry is at present cultivated photosynthetic bacterium thing device and is generally horizontal rectangular cylinder body, and not only floor space is big, and function singleness, is not easy to stir culture liquid and removes the container inner wall dirt settling, has reduced light utilization efficiency, has influenced production efficiency and quality.
The purpose of this utility model is to propose a kind of vertical photosynthetic bacterium culture apparatus, not only floor space is less, can make full use of light source, and tool provides functions such as artificial lighting with stirring, remove dirt settling, improve quality product and production efficiency effectively, be fit to scale operation.
The purpose of this utility model is to realize like this, the vertical just rectangular-shaped or vertical oval tubbiness cylinder body that adopts withstand voltage light transmissive material to make, cylinder body is supported on the support of being made by the angle bar weldering, cylinder base is provided with baiting valve, cylinder body is marked with the volume scale, its open top, cover the cylinder cap of making by light transmissive material that studs with rubber seal, a screw rod is installed between cylinder cap and the bottom surface, connect the cylinder brush by threaded hole, the cylinder brush combines closely with inboard wall of cylinder block, and the screw rod lower end is equipped with agitator, shake the handle that install the screw rod upper end, the drive cylinder brushes down to move with agitator and rotates; Solution input tube and outside lamp bracket are installed on the support.Adopt above scheme and structure, reduced the floorage of culture apparatus, both diminishbb floor space can make it be subjected to light to increase light-receiving area again; And realized the input of solution and output, indicated liquor capacity, stirring, removing inwall dirt settling and function such as artificial lighting is provided.
Below in conjunction with accompanying drawing the utility model is described in detail:
Fig. 1 is the stereographic map of the utility model one embodiment.
Fig. 2 is the utility model sectional view of removing support embodiment illustrated in fig. 1.
1. conveying pipelines, 2. flexible pipes, 3. cylinder caps, 4. conveying lids, 5. sealing rings, 6. walls are brushed 7. screw rods, 8. cylinder bodies, 9. agitators, 10. supports, 11. screw rod square heads, 12. wing nuts, 13. cylinder cap connecting ears, 14. cylinder body connecting ears, 15. bulbs, 16. lamp brackets, 17. rotating seats, 18. baiting valves, 19. lock-screws, 20. walls brush screwed hole, 21. wall brush holders, 22. brushes, 23. sleeves, 24. handles, 25. handle square toes holes, 26. steps, 27. drain holes, 28. rubber rings, 29. conveying mouths, 30. containing marks among the figure
Shown in figure (1), (2), this embodiment of the utility model is made up of cylinder body (8), cylinder cap (3), support (10), screw rod (7), wall brush (6), agitator (9) and handle parts such as (24), cylinder body is bonding the forming of synthetic glass of 8mm by thickness, be carved with volume scale (30) on its outer wall, its physical dimension is the rectangular parallelepiped of 300 * 300 * 1500mm, be supported on the support (10) by angle bar weldering system, have drain hole (27) on the bottom surface of cylinder body, baiting valve (18) can be installed, and the center design of bottom surface has rotary seat (17).Outer wall of cylinder block can have containing mark (30), can read the volume of nutrient solution in the cylinder body.Cylinder cap (3) passes through connecting ear (13), (14) through bolted-on connection with cylinder body (8), cylinder cap has groove on (3), sealing-ring (5) is set in wherein, can cylinder cap (3) and cylinder body (8) is tight-closed when tightening wing nut (12), cylinder cap (3) central authorities have circular hole, and cylinder cap has a defeated material mouthful (29) in addition, flexible pipe (2) can be put into cylinder body (8) input solution, defeated material mouthful (a 29) loam cake has been put defeated material flap (4), is lined with rubber layer below it to compress through locking screw (19).Screw rod (7) is installed between cylinder cap (3) medium pore and the bottom surface rotary seat (17), its upper end design has one section square head (11), one section right cylinder and step (26) are arranged below, right cylinder is through in cylinder cap (3) central circular hole, is subjected to step (26) spacing, is plugged with rubber ring (28) at the circular hole place in order to prevent entering of air, step can be brushed threaded hole (20) with wall for thread segment down and link, be with a sleeve (23) under the thread segment, the sleeve below is a four directions section, and its below is the bulb right cylinder.There is a cubic hole to be through on the four directions section of screw rod lower end in the middle of the agitator (9), the bulb cylinder is inserted in the circular hole of rotary seat (17), agitator this moment (9) is sandwiched between sleeve (23) and the bottom surface just, agitator (9) is made up of 2-4 blade, blade is up-narrow and down-wide, brush is installed under the blade contacts with the cylinder body bottom surface, agitator when screw rod rotates (9) and then rotation not only plays stirring action but also can remove the dirt settling of bottom surface.The brush (22) of wall brush is made the square identical with cross section in the cylinder body and is closely contacted with inboard wall of cylinder block, brush is through wall brush holder (21) support and connection, wall brush holder (21) is made concave shaped, the threaded hole (20) of wall brush (6) top connects with screw rod, when screw rod rotated, wall brush (6) moved up and down, can be with cylinder body (8) inwall wiped clean, wall brush holder (21) is made concave shaped, can prevent to interfere with agitator (9).Handle (24) one ends designs has cubic hole (25), can be enclosed within on the screw rod square head (11), shake that handle (24) can drive threaded rod (7) thus rotation drives agitator (9) rotation and wall brush (6) to move up and down to play and stirs and the effect of removing dirt settling.Opposite spin handle when the wall brush moves to upper end or lower end.In addition, be fixed with lamp bracket (16) on the support (10) and electric light (15) can be installed light source is provided, also be fixed with carrier pipe (1) on the support and connect flexible pipe (2), be convenient to import solution.
When using the utility model, can take down handle (24) earlier unscrews wing nut (12) and takes off cylinder cap (3), behind cylinder body (8) inwall and various additional device cleaning and sterilizing, cover cylinder cap (3), in cylinder body (8), import required solution and reagent by carrier pipe (1) and flexible pipe (2) through the defeated material mouth (29) of cylinder cap, shaking handle (24) drive agitator (9) rotation stirs nutrient solution, if dirt settling appears in the body inwall, also can shake on handle (24) the drive wall brush (6) and move or move down, thereby remove the dirt settling on the inboard wall of cylinder block.Finish when solution and reagent add, turn defeated material flap (4) covers defeated material mouthful (29) completely and tightens locking screw (19); When stopping to shake, rubber ring (28) jam-pack should be prevented air admission.
The utility model adopts vertical structure, has increased effective area of shining light and has reduced occupation of land than the horizontal type structure with volume Area has reduced the dissolved oxygen in the nutrient solution; The agitator (9) of design and wall brush (6) have been removed attachment effectively And nutrient solution is stirred, improved light transmission; Adopt sealing ring (5), cylinder between cylinder cap (3) and the cylinder body (8) Be lined with rubber layer under lid conveying mouth (29) lid, be plugged with rubber ring (28) between cylinder cap centre bore and screw rod (7), effectively Prevented the infiltration of air. The utility model can take full advantage of natural lighting and rationally use artificial lighting, is conducive to photosynthetic The growth of bacterium, the photosynthetic bacteria liquid bacterium number of cultivation can reach 60 * 108CFU/ml has improved the productivity ratio of photosynthetic bacteria And quality, be applicable to large-scale production.

Claims (7)

1, a kind of vertical photosynthetic bacterium culture apparatus, mainly form by cylinder body (8), cylinder cap (3), support (10), screw rod (7), wall brush (6), agitator (9) and handle parts such as (24), it is characterized in that cylinder body (8) is supported on the support (10), cylinder body (8) and cylinder cap (3) are through bolted-on connection, agitator (9) and wall brush (6) is installed on cylinder body (8) the intermediary screw rod (7), handle (24) connects with screw rod square head (11), shake handle (24) agitator (9) and rotate thereupon, wall brush (6) above-below direction thereupon moves.
2, vertical photosynthetic bacterium culture apparatus according to claim 1, it is characterized in that its cylinder body (8) is vertical positive rectangular parallelepiped or vertical oval tubbiness, be carved with volume scale (30) on its outer wall, cylinder body (8) and cylinder cap (3) all have withstand voltage light transmissive material to make, have drain hole (27) on the bottom surface of cylinder body, baiting valve (18) can be installed, and the center design of bottom surface has rotary seat (17), has a circular hole in the rotary seat.
3, vertical photosynthetic bacterium culture apparatus according to claim 1 is characterized in that having groove on its cylinder cap (3), and sealing-ring (5) is set in wherein, and design has connecting ear (13) and cylinder body (8) connecting ear (14) through bolted-on connection.Sealing; Cylinder cap (3) center has a circular hole, and screw rod (7) upper end column part can penetrate, and is plugged with rubber ring (28) sealing; Also have drain hole (29) on the cylinder cap (3), be stamped the blowing flap (4) that is lined with rubber pad on it, connect through locking screw (19).
4, vertical photosynthetic bacterium culture apparatus according to claim 1 is characterized in that its support (10) is formed by angle bar weldering system, is equipped with lamp bracket (16) and carrier pipe (1) on the support (10); Lamp bracket (16) can be installed electric light (15), and carrier pipe (1) can connect flexible pipe (2).
5, vertical photosynthetic bacterium culture apparatus according to claim 1, it is characterized in that there is square head (11) its screw rod (7) upper end, be a cylindrical section below, it below the cylindrical section step (26), step is thread segment down, be with a sleeve (23) under the thread segment, it is four directions section and bulb cylindrical section down.
6, vertical photosynthetic bacterium culture apparatus according to claim 1, it is characterized in that its agitator (9) is made up of 2-4 blade, blade is up-narrow and down-wide, brush is installed under the blade contacts with the cylinder body bottom surface, there is square hole the centre, is enclosed within just on the section of four directions, screw rod (7) lower end.
7, vertical photosynthetic bacterium culture apparatus according to claim 1 is characterized in that the brush (22) of its wall brush (6) is made and the identical shape in cylinder body (8) cross section, and contacts closely with cylinder body (8) inwall that brush (22) supports by wall brush holder (21); Wall brush holder (21) top has threaded hole (20) and connects with screw rod (7), and wall brush holder (21) is made concave shape, can cover on the agitator (9), does not interfere with agitator (9) when moving to cylinder body (8) bottom surface.
CNU032209045U 2003-04-01 2003-04-01 Vertical photosynthetic bacterial culture apparatus Expired - Fee Related CN2616547Y (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102226146A (en) * 2011-05-20 2011-10-26 金华职业技术学院 Photobiological reaction equipment of microalgae
CN102807952A (en) * 2012-08-14 2012-12-05 中国人民解放军南京军区南京总医院 Rocking dynamic culture box
CN105754844A (en) * 2016-04-19 2016-07-13 赵美玲 Microbial culture device
CN109880740A (en) * 2019-03-26 2019-06-14 蔡玉华 A kind of culture of photosynthetic bacteria, detection device and application method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102226146A (en) * 2011-05-20 2011-10-26 金华职业技术学院 Photobiological reaction equipment of microalgae
CN102807952A (en) * 2012-08-14 2012-12-05 中国人民解放军南京军区南京总医院 Rocking dynamic culture box
CN102807952B (en) * 2012-08-14 2014-08-27 中国人民解放军南京军区南京总医院 Rocking dynamic culture box
CN105754844A (en) * 2016-04-19 2016-07-13 赵美玲 Microbial culture device
CN109880740A (en) * 2019-03-26 2019-06-14 蔡玉华 A kind of culture of photosynthetic bacteria, detection device and application method
CN109880740B (en) * 2019-03-26 2020-09-01 蔡玉华 Culture and detection device for photosynthetic bacteria and use method

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