CN2567227Y - Fermentation device for solid state fermentation sterile operation - Google Patents
Fermentation device for solid state fermentation sterile operation Download PDFInfo
- Publication number
- CN2567227Y CN2567227Y CN02245659U CN02245659U CN2567227Y CN 2567227 Y CN2567227 Y CN 2567227Y CN 02245659 U CN02245659 U CN 02245659U CN 02245659 U CN02245659 U CN 02245659U CN 2567227 Y CN2567227 Y CN 2567227Y
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- Prior art keywords
- state fermentation
- solid
- fermentation
- solid state
- incubator
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
Abstract
The utility model relates to a fermentation device for solid state fermentation sterile operation, which comprises a liquid seed tank which is communicated with a solid medium sterilizing tank with a lower cap through a pipe with a valve, wherein, the wall of the solid medium sterilizing tank is connected with a pipe with a valve, and the lower port of the solid medium sterilizing tank is communicated with a seed receiving barrel through a 100 level ultra-clean platform. The lower end port of the seed receiving barrel is installed on a 100 level ultra-clean chamber, a seed receiving barrel of a mechanical drum sieve is arranged under the lower end port of the receiving barrel, and a shallow incubation pan is arranged on the lower port of the seed receiving barrel of a mechanical drum sieve. The shallow incubation pan, or a honeycomb incubator, or a belt type incubator is communicated with a solid state fermentation reactor with an air inlet through a traction device. The utility model starts with processes of seed preparation, culture medium sterilization, aseptic inoculation, aseptic material loading, aseptic solid state fermentation, etc., and forms a whole aseptic technique of solid state fermentation, to solve the problem of contamination in a solid state fermentation process.
Description
Technical field
The utility model relates to a kind of fermentation unit, particularly a kind of fermentation unit of solid state fermentation aseptic technique.
Background technology
Solid state fermentation is meant that substratum is solid-state, though it is moisture abundant, but there are not or almost do not have one or more fermentation process of carrying out under the state of free water, substrate (matrix) is the water-insoluble solid mixture, it not only can provide the required carbon source of microorganism, nitrogenous source, inorganic salt, water and other nutrition, or the place of microorganism growth.The most outstanding advantage of solid state fermentation is that technological process is simple, less investment, high production, and its concrete characteristics can be summarized as follows: (1) fermentation costs is low, and the raw material cheapness can directly utilize industrial or agricultural residue (cellulose raw material recyclability resource) etc. as substrate; (2) simulating nature growing environment makes microorganism keep the growth conditions similar to nature; (3) there is not three waste discharges such as " waste water, waste residue, waste gas "; (4) the product aftertreatment is simple, is easy to characteristics such as storage, transportation.Before several thousand, China just utilizes this technology wine brewing and makes various seasonings.So far it is still the main mode of producing traditional food and beverage.Solid state fermentation can the production zymin, single cell protein, organic acid, alcohol and biotic pesticide etc.Characteristics such as fermentation costs is low because solid state fermentation has, and cost of material is cheap, does not have " three wastes " discharging, and environmental pollution is little, and technological process is simple at present, progressively obtain the attention of countries in the world.
Though solid state fermentation has one of the longest technology, the middle of this century, along with the discovery of penicillin, the rise gradually of microbiotic industry has driven the development of fermentation industry; World War II has aggravated the mass-produced needs of penicillin, and this requires fermentation technique at that time can realize also must accomplishing purebred cultivation simultaneously outside the large-scale industrial production.And traditional solid state fermentation can't fundamentally solve microbiological contamination dip-dye problem, so do not obtain enough attention and development under historical conditions at that time.The deep fermentation technology be owing to can realize purebred cultivation of microorganism and large-scale production, meets the demand of historical development and obtains systematic research and use, develops very swift and violent.But also there are many problems in liquid submerged fermentation, and for example: the fermentation costs height, strive grain, environmental pollution etc. with people and animals, these drawbacks are obviously inconsistent with the development of modern fermentation industry, become and limit the unfavorable factor that it further develops.
Modern fermentation industry makes the liquid submerged fermentation technology occupy dominant position to the strict demand of purebred cultivation of microorganism and scale operation.Solid-state fermentation process and equipment are because of its easy microbiological contamination, and fermentation condition is restive, and reasons such as difficulty are amplified in industry, are considered to a kind of ancient and backward fermentation process always.But the plurality of advantages of solid state fermentation is brief as technical process, and raw material sources are extensive, cheap, and energy consumption is low, and is pollution-free etc., in the bio-reactor development, has powerful magnetism and vitality.The key of solid state fermentation problem is the microbiological contamination problem that solves in the solid ferment process, and the aseptic technique of solid state fermentation relates to from a series of links such as seed preparation, medium sterilization, aseptic inoculation, germ-free charging and aseptic solid-state fermentation reactors.
Summary of the invention
One of the purpose of this utility model is to overcome solid-state fermentation process and equipment because of its easy microbiological contamination, and fermentation condition is restive, and the shortcoming of difficulty is amplified in industry; For lag period and the seed production cycle that shortens solid ferment process, avoid infectious bacteria in the fermenting process; Thereby the utility model provides a kind of fermentation unit of solid state fermentation aseptic technique.
The purpose of this utility model is achieved in that
The fermentation unit of the solid state fermentation aseptic technique that the utility model provides comprises: liquid seeds jar, inoculation tube and solid-state fermentation reactor; It is characterized in that: comprise that also one has the solid medium sterilization tank of lower cover, inoculation tube, cultivation tray, cellular incubator, conveyor type incubator, 100 grades of ultra-clean chambers and 100 grades of ultra-clean service platforms of machinery drum sieve; Wherein the liquid seeds jar is communicated with the solid medium sterilization tank that has lower cover by the pipeline that has valve, be connected to a pipeline that has valve on the wall of this solid medium sterilization tank, lower port at described solid medium sterilization tank is communicated with the inoculation tube by described 100 grades of ultra-clean service platforms, the lower port of inoculation tube is installed on 100 grades of ultra-clean chambers, the below of this lower port is provided with an aseptic inoculation tube that has a loam cake, and this inoculation tube is by on 00 grade of ultra-clean chamber inwall of anchor stuck-at-; The end opening of inoculation tube is provided with one and cultivates tray, and this cultivation tray is placed on the tray frame, on 00 grade of ultra-clean chamber inwall of its tray frame stuck-at-; Cultivate the following cellular incubator that also can be provided with of tray, this cellular incubator is placed on the interior mechanical haulage device of 100 grades of ultra-clean chambers; One conveyor type incubator also can be set below its cellular incubator, and 100 grades of ultra-clean chambers are connected with the solid-state fermentation reactor that has inlet mouth, and cellular incubator is connected with the mechanical haulage device respectively with the conveyor type incubator.
Described solid medium sterilization tank is the pressurized vessel of a configuration heating or cooling jacket, and can 4-10 rev/min of rotation up and down, and jar tapered up and down.
Described liquid seeds jar is conventional deep liquid standard fermentor tank;
Described 100 grades of ultra-clean chambers 11 and 100 grades of ultra-clean platforms are by the processing and manufacturing of EMP standard;
Described aseptic inoculation tube is the vertical pressurized vessel of placing, and in order to prevent and treat the sticking aseptic inoculation barrel of material, portion prevents to dismantle the interior cloth tube of aseptic inoculation tube of washing within it, before the aseptic inoculation tube uses, and 120 ℃ of steam sterilizing 30min;
Described inoculation tube is a bucket-shaped container all by the stainless material manufacturing, and container bottom is provided with dodge gate, and machinery drum sieve is installed in it.
Described cultivation tray, cellular incubator, conveyor type incubator are meant three kinds of different charging methods.
Described cultivation tray is meant discharging tray cribbing in solid-state fermentation reactor, places the thick solid medium of 2-5cm on tray.
Described cellular incubator is meant the cellular cribbing of discharging in solid-state fermentation reactor, the solid medium of stacking in cellular incubator.
Described conveyor type incubator is meant discharge conveyor formula travel(l)ing rest in solid-state fermentation reactor, stacks the thick solid medium of 2-5cm on travelling belt.
Described solid-state fermentation reactor is a seal-off pressure container, solid-state fermentation reactor that can 120 ℃ of steam sterilizings.
For lag period and the seed production cycle that shortens solid ferment process, the utility model is selected conventional liq fermentative preparation solid state fermentation seed liquor for use, also claims liquid-solid zymotechnique.
Advantage of the present utility model: (1) adopts tapered solid medium sterilization tank to carry out the solid medium sterilization and inoculation mixes, and solved that extensive solid medium is difficult to sterilize and the problem of extensive aseptic inoculation; (2) adopt 100 grades of ultra-clean chambers and 100 grades of ultra-clean platforms to solve that extensive solid culture material transmits and operating process in easy microbiological contamination problem; (3) adopting the interior cloth tube of vertical aseptic inoculation tube of placing and the aseptic inoculation tube that can dismantle washing to solve extensive solid culture material is difficult to carry and sticking wall problem; (4) adopt machinery drum sieve inoculation tube to solve the problem of easy conglomeration of extensive solid culture material and the easy microbiological contamination of artificial inoculation; (5) adopt the mechanical haulage tray to place the crosshead or the conveyor type incubator of tray frame or cellular incubator, avoid culture to contact, thereby solved the problem of the easy microbiological contamination of extensive solid culture material manual operation with outside; (6) adopt seal-off pressure container solid-state fermentation reactor to solve microbiological contamination problem in the solid ferment process.
Description of drawings 1. apparatus structure synoptic diagram of the present utility model (being arranged vertically) 1, liquid seeds jar, 2, solid medium sterilization tank, 3, solid medium sterilization tank chuck, 4, aseptic inoculation tube, 5, cloth tube in the aseptic inoculation tube, 6, trommel screen, 7, trommel screen inoculation tube, 8, cultivate tray, 9, cellular incubator, 10, the conveyor type incubator, 11,100 grades of ultra-clean chambers, 12,100 grades of ultra-clean service platforms, 13, solid-state fermentation reactor
Embodiment
Embodiment 1
Make the fermentation isolated plant that a cover adopts liquid-solid bonded aseptic technique by Fig. 1, comprising: the deep liquid standard fermentor tank of a standard is as liquid seeds jar 1; Inoculation tube 4 and solid-state fermentation reactor 13; Solid medium sterilization tank 2, this solid medium sterilization tank are the pressurized vessels of a configuration heating or cooling jacket, and can 4-10 rev/min of rotation up and down, and jar tapered up and down; Machinery drum sieve inoculation tube 7, cultivation tray 8, cellular incubator 9,10,100 grades of ultra-clean chambers 11 of conveyor type incubator and 100 grades of ultra-clean service platforms 12 are by the processing and manufacturing of EMP standard; Wherein liquid seeds jar 1 is communicated with solid medium sterilization tank 2 by the pipeline 14 that has valve, be connected to a pipeline 15 that has valve on the wall of solid medium sterilization tank 2, lower port at solid medium sterilization tank 2 is communicated with inoculation tube 4 by 100 grades of ultra-clean service platforms 12, aseptic inoculation tube 4 is vertical pressurized vessels of placing, in order to prevent and treat the sticking aseptic inoculation barrel of material, portion prevents to dismantle the interior cloth tube 5 of aseptic inoculation tube of washing within it, before the aseptic inoculation tube uses, 120 ℃ of steam sterilizing 30min; The lower port of inoculation tube 4 is installed on 100 grades of ultra-clean chambers 11, the below of this lower port is provided with the inoculation tube 7 of a band machinery drum sieve 6, the described inoculation tube 7 of machinery drum sieve that has is all by the stainless material manufacturing, and trommel screen 6, the wall of trommel screen inoculation tube 7 is convenient to disassembly, cleaning, does not have the dead angle; The end opening of inoculation tube 7 is provided with one and cultivates tray 8, cultivate tray 8 below a cellular incubator 9 is set, this cellular incubator is placed on the mechanical haulage device in 100 grades of ultra-clean chambers; 10,100 grades of ultra-clean chambers of one conveyor type incubator are set below its cellular incubator 9 are connected with the solid-state fermentation reactor 13 that has inlet mouth, cellular incubator is connected with the mechanical haulage device respectively with the conveyor type incubator.To carry out solid-state cultivation in the solid-state fermentation reactor of cultivating after tray 8, cellular incubator 9 or conveyor type incubator 10 are sent into autoclaving 13.
By the seed liquor of liquid fermentation tank 1 preparation, improve the pressure of liquid fermentation tank by sterile air, seed liquor is transported in the solid medium sterilization tank 2 behind the autoclaving; Solid medium sterilization tank 2 rotations 3-6 time make liquid seeds liquid and solid medium mixing of materials even; In 100 grades of ultra-clean service platforms 12, rapidly solid medium sterilization tank 2 lower covers and aseptic inoculation tube 4 loam cakes are opened, and be connected to the solid medium sterilization tank with cloth tube 5 in the aseptic inoculation tube and cover for 2 times, the material vertical drop that mixes in the solid medium sterilization tank is in aseptic inoculation tube 4; In 100 grades of ultra-clean chambers 11, aseptic inoculation tube 4 lower covers are opened, be charged to by machinery drum sieve inoculation tube 7 and cultivate on tray 8 or cellular incubator 9 or the conveyor type incubator 10, place in the seal-off pressure container tray solid-state fermentation reactor of tray frame behind the steam sterilizing conveyor type incubator by the mechanical haulage tray and carry out solid state fermentation and cultivate.
Embodiment 2:
Carrying out solid state fermentation by the solid-state fermentation reactor of the cellular charging method of seal-off pressure container of the cellular incubator crosshead of mechanical haulage behind the steam sterilizing cultivates.Other operating process is with embodiment 1.
Embodiment 3:
The operating process of present embodiment is with embodiment 1.Difference is that carrying out solid state fermentation in the solid-state fermentation reactor by the seal-off pressure container conveying belt charging method of mechanical haulage conveyor type incubator behind the steam sterilizing cultivates.
Claims (8)
1. the fermentation unit of a solid state fermentation aseptic technique comprises: liquid seeds jar 1, inoculation tube 4 and solid-state fermentation reactor 13; It is characterized in that: inoculation tube 7, cultivation tray 8, cellular incubator 9,10,100 grades of ultra-clean chambers 11 of conveyor type incubator and 100 grades of ultra-clean service platforms 12 of also comprising solid medium sterilization tank 2, machinery drum sieve 6; Wherein liquid seeds jar 1 is communicated with the solid medium sterilization tank 2 that has lower cover by the pipeline 14 that has valve 16, be connected to a pipeline 15 that has valve 17 on the wall of solid medium sterilization tank 2, lower port at solid medium sterilization tank 2 is communicated with inoculation tube 4 by 100 grades of ultra-clean service platforms 12, the lower port of inoculation tube 4 is installed on 100 grades of ultra-clean chambers 11, the below of this lower port is provided with the inoculation tube 7 of machinery drum sieve 6, the end opening of inoculation tube 7 is provided with one and cultivates tray 8, cultivate the following cellular incubator 9 that also can be provided with of tray 8, this nest honeybee shape incubator 9 is placed in interior the drawing of 100 grades of ultra-clean chambers and leads on the device; One conveyor type incubator 10 also can be set, and its 100 grades of ultra-clean chamber 11 that are connected with the mechanical haulage device is connected with the solid-state fermentation reactor 13 that has inlet mouth.
2. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described solid medium sterilization tank is the pressurized vessel of a configuration heating or cooling jacket 3, and can 4-10 rev/min of rotation up and down, and tank body is tapered up and down.
3. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described inoculation tube 4 is vertical aseptic pressurized vessels of placing, and portion is provided with cloth tube 5 within it.
4. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described inoculation tube is a bucket-shaped container all by the stainless material manufacturing, and container bottom is provided with dodge gate, and machinery drum sieve is installed in it.
5. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described cultivation tray is meant discharging tray cribbing in solid-state fermentation reactor, places the thick solid medium of 2-5cm on tray.
6. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described cellular incubator is meant the cellular cribbing of discharging in solid-state fermentation reactor, the solid medium of stacking in cellular incubator.
7. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described conveyor type incubator is meant discharge conveyor formula travel(l)ing rest in solid-state fermentation reactor, stacks the thick solid medium of 2-5cm on travelling belt.
8. by the fermentation unit of the described solid state fermentation aseptic technique of claim 1, it is characterized in that: described solid-state fermentation reactor 13 be meant can 120 ℃ of steam sterilizings the seal-off pressure container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02245659U CN2567227Y (en) | 2002-08-09 | 2002-08-09 | Fermentation device for solid state fermentation sterile operation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02245659U CN2567227Y (en) | 2002-08-09 | 2002-08-09 | Fermentation device for solid state fermentation sterile operation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2567227Y true CN2567227Y (en) | 2003-08-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN02245659U Expired - Lifetime CN2567227Y (en) | 2002-08-09 | 2002-08-09 | Fermentation device for solid state fermentation sterile operation |
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| CN (1) | CN2567227Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911282A (en) * | 2013-01-06 | 2014-07-09 | 北大工学院绍兴技术研究院 | Fermentation apparatus |
| CN104195032A (en) * | 2014-08-24 | 2014-12-10 | 侯梦斌 | Equipment and process for microorganism solid material fermentation |
| CN106061513A (en) * | 2014-01-24 | 2016-10-26 | 皮埃尔·法布尔皮肤化妆品公司 | Device and method for transferring a sterile product between two containers |
-
2002
- 2002-08-09 CN CN02245659U patent/CN2567227Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911282A (en) * | 2013-01-06 | 2014-07-09 | 北大工学院绍兴技术研究院 | Fermentation apparatus |
| CN106061513A (en) * | 2014-01-24 | 2016-10-26 | 皮埃尔·法布尔皮肤化妆品公司 | Device and method for transferring a sterile product between two containers |
| CN106061513B (en) * | 2014-01-24 | 2019-11-12 | 皮埃尔·法布尔皮肤化妆品公司 | Device and method for transmitting sterile product between the two containers |
| CN104195032A (en) * | 2014-08-24 | 2014-12-10 | 侯梦斌 | Equipment and process for microorganism solid material fermentation |
| CN104195032B (en) * | 2014-08-24 | 2016-02-10 | 侯梦斌 | A kind of microorganism expects the equipment and technology fermented admittedly |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050921 |
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| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050921 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |