CN219878717U - Sterilizing box for plant tissue culture - Google Patents
Sterilizing box for plant tissue culture Download PDFInfo
- Publication number
- CN219878717U CN219878717U CN202321084362.4U CN202321084362U CN219878717U CN 219878717 U CN219878717 U CN 219878717U CN 202321084362 U CN202321084362 U CN 202321084362U CN 219878717 U CN219878717 U CN 219878717U
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- CN
- China
- Prior art keywords
- filter
- screw
- cover
- box
- box body
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 230000000694 effects Effects 0.000 abstract description 9
- 238000009423 ventilation Methods 0.000 abstract description 7
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- 239000011521 glass Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 abstract description 4
- 238000011084 recovery Methods 0.000 abstract description 3
- 238000003860 storage Methods 0.000 abstract description 3
- 230000000149 penetrating effect Effects 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 5
- 238000012258 culturing Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The utility model relates to the technical field of plant tissue culture, and discloses a sterilizing box for plant tissue culture, which comprises a box body and a box cover, wherein the box cover is buckled and sealed above the box body, screw holes are formed in two sides of the surface of the box cover, a filter is connected above the screw holes through a screw cylinder, the filter is communicated with the other side of the box cover, a microporous filter plate is attached to the top surface of the filter, the microporous filter plate is locked above the filter through an annular screw cover connected above the filter, and the middle of the annular screw cover is in a penetrating state. According to the technical scheme, the disposable plastic box body is matched with the box cover to realize the sealing effect, and the filter is arranged to keep ventilation inside the box body, so that bacterial pollution is avoided, plant tissue is cultivated, the problems of high purchase cost, high recovery and cleaning cost, laborious transportation, easiness in breakage, huge storage space and the like of the glass bottle are effectively avoided, and the production efficiency is greatly improved.
Description
Technical Field
The utility model relates to the technical field of plant tissue culture, in particular to a sterilizing box for plant tissue culture.
Background
The plant tissue culture is a technology for culturing isolated plant organs, tissues, cells and protoplasts by utilizing a proper culture medium under aseptic and manual control environment conditions, so that the cells or the whole plants are regenerated, and the preparation of the aseptic culture medium is an essential link of plant tissue culture.
In actual production, the main flow path is to adopt glass bottles, pour culture medium and then seal and sterilize, which brings the problems of large-scale tissue culture production that a large amount of glass bottles are required to be purchased and a large amount of manpower is required to be used for cleaning. Therefore, we propose a sterilization box for plant tissue culture.
Disclosure of Invention
The utility model aims to provide a sterilizing box for plant tissue culture, which is characterized in that a disposable plastic box body is adopted, a box cover is matched to realize a sealing effect, a filter is arranged to keep ventilation inside the box body, meanwhile, bacterial pollution is avoided, plant tissue culture is realized, the problems of high purchase cost, high recovery and cleaning cost, laborious transportation, easiness in breakage, huge storage space and the like of a glass bottle are effectively avoided, the production efficiency is greatly improved, and the problems in the background technology are solved.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the sterilizing box for plant tissue culture comprises a box body and a box cover, wherein the box cover is buckled and sealed above the box body, and screw holes are formed in two sides of the surface of the box cover; the upper part of the screw hole is connected with a filter through a screw cylinder, the filter is communicated with the other side of the box cover, a microporous filter plate is attached to the top surface of the filter, the microporous filter plate is locked above the filter through an annular screw cover connected above the filter, and the middle of the annular screw cover is in a through state; the lower outer ring of the box cover is provided with a ring groove in a surrounding mode, the upper Fang Waijuan of the box body is clamped in the ring groove, four sides of the box cover are integrally connected with buckles, and the buckles are buckled on the buckle plates of the outer ring of the box body.
By adopting the technical scheme, each component is sterilized, then assembled, plant tissues and culture solution are added into the box body after being cooled, and then the box cover is sealed for culturing the plant tissues, so that the sterile environment and ventilation effect in the culturing process are maintained.
Optionally, a gasket is fixed around the outer ring of the filter above the screw cylinder and is abutted against the upper surface of the box cover.
Through adopting above-mentioned technical scheme, when the screw shell is screwed into the screw, the gasket is contradicted and can be realized spacing effect at the lid surface.
Optionally, a sealing ring is embedded and fixed on the inner surface of the ring groove, and the upper surface of the box body abuts against the surface of the sealing ring.
Through adopting above-mentioned technical scheme, the sealing washer is contradicted between annular and box body upper surface, has the effect that improves sealed effect.
Optionally, the annular screw cap is connected to the upper outer ring of the filter in a screwing mode, and the inner part of the annular screw cap is tightly pressed on the surface of the microporous filter plate.
Through adopting above-mentioned technical scheme, the detachable setting of annular screw cap for its inboard fixed micropore filter plate also can be dismantled, conveniently prevents fungus to it and deposits.
Optionally, the inner ring of the screw hole and the outer ring of the screw barrel are both provided with threads, and the screw barrel is screwed into the screw hole through the threads.
By adopting the technical scheme, the purpose that the screw barrel is screwed into the screw hole is realized by utilizing the interaction between the threads.
Compared with the prior art, the technical scheme of the utility model has the following beneficial effects:
1. according to the technical scheme, the disposable plastic box body is matched with the box cover to realize the sealing effect, and the filter is arranged to keep ventilation inside the box body, so that bacterial pollution is avoided, plant tissue is cultivated, the problems of high purchase cost, high recovery and cleaning cost, laborious transportation, easiness in breakage, huge storage space and the like of the glass bottle are effectively avoided, and the production efficiency is greatly improved.
2. According to the technical scheme, the detachable filter is arranged above the box cover, so that the ventilation effect inside the box body can be realized when plant tissues are cultured, and bacteria entering the box body are prevented from being polluted.
Drawings
Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic diagram showing the overall structure of a sterilization case for plant tissue culture according to the present utility model;
FIG. 2 is a schematic view showing the structure of the upper part of the filter of the sterilizing cassette for plant tissue culture of the present utility model;
FIG. 3 is a schematic diagram showing the detailed structure of the sterilization case for plant tissue culture at A.
In the figure: 1. a case body; 11. a buckle plate; 2. a box cover; 21. a screw hole; 22. a buckle; 3. a filter; 31. a screw cylinder; 32. a gasket; 33. an annular screw cap; 34. a microporous filter plate; 4. a ring groove; 41. and (3) sealing rings.
Detailed Description
Referring to fig. 1-3, the present utility model provides a technical solution: the utility model provides a plant tissue culture is with sterilizing box, including box body 1 and lid 2, lid 2 buckle seals in the top of box body 1, the equal holistic buckle 22 that is connected with in four sides of lid 2, the holistic buckle 11 that is provided with in box body 1 outer lane that is located buckle 22 below makes buckle 22 buckle arrange the buckle 11 in box body 1 outer lane, and then compress tightly the locking with lid 2 in box body 1 top, the below outer lane of lid 2 is encircleed is provided with annular groove 4, and annular groove 4's inside embedding is fixed with annular sealing washer 41, make box body 1 go up Fang Waijuan snap-in annular groove 4 and support tightly at sealing washer 41's surface, can realize the sealed locking between lid 2 and the box body 1 like this.
Screw holes 21 are formed in two sides of the surface of the box cover 2, a screw cylinder 31 is screwed into the screw holes 21, the screw cylinder 31 is communicated with the other side of the box cover 2, a filter 3 is fixedly connected to the whole upper portion of the screw cylinder 31, a supporting piece is integrally arranged on the outer ring of the filter 3 above the screw cylinder 31 for realizing the limiting effect of screwing the screw cylinder 31 into the screw holes 21, and after the screw cylinder 31 is screwed into a sufficient depth to ensure stable connection with the screw holes 21, a gasket 32 is abutted against the surface of the box cover 2 for limiting.
The filter 3 is provided with the microporous filter plate 34 in a pasting mode on the top surface, the diameter of the microporous filter plate 34 is slightly larger than the inner diameter of the opening at the top of the filter 3 and smaller than the inner diameter of the annular screw cover 33 arranged above the microporous filter plate 34, so that the annular screw cover 33 can be pressed above the microporous filter plate 34, and is screwed above the filter 3, the microporous filter plate 34 is locked above the filter 3, and the middle of the annular screw cover 33 is in a vertical through state, so that the ventilation state inside the box body 1 can be realized through the filter 3, and the microporous filter plate 34 is utilized for filtering, bacterial pollution inside the box body 1 is avoided, and the microporous filter plate 34 can be independently stored before use due to the detachable arrangement of the annular screw cover 33 and the microporous filter plate 34, so that the microporous filter plate which is more easy to be adhered with the bacteria is polluted before use is avoided, and different types of filter plates can be replaced according to requirements.
When the novel sterilizing box is used, sterilization treatment is carried out on each structure, then the screw cylinder 31 at the bottom of the filter 3 is screwed into the screw hole 21 until the gasket 32 is abutted against the upper surface of the box cover 2, the purpose of connecting the filter 3 to the two sides above the box cover 2 is achieved, after the filter is cooled until a culture medium is solidified, the culture medium is opened on an ultra-clean workbench, plant tissues and culture solution can be put into use, the box body 1 is placed, then the box cover 2 is covered, the upper part of the box body 1 is clamped into the annular groove 4 of the box cover 2 and is contacted with the sealing ring 41, the buckles 22 on the four sides of the box cover 2 are clamped on the buckle plate 11 on the outer ring of the box body 1, the box cover 2 can be tightly locked above the box body 1, then the plant tissues can be cultured, and the filters 3 on the two sides can filter gas while keeping the ventilation effect, and bacterial pollution is avoided to the box body 1.
Claims (5)
1. The utility model provides a plant tissue culture is with sterilizing box, includes box body (1) and lid (2), its characterized in that: the box cover (2) is buckled and sealed above the box body (1), and screw holes (21) are formed in two sides of the surface of the box cover (2);
the upper part of the screw hole (21) is connected with a filter (3) through a screw cylinder (31), the filter (3) is communicated with the other side of the box cover (2), a microporous filter plate (34) is attached to the top surface of the filter (3), the microporous filter plate (34) is locked above the filter (3) through an annular screw cover (33) connected above the filter (3), and the middle of the annular screw cover (33) is in a through state;
the box cover is characterized in that an annular groove (4) is formed in the lower outer ring of the box cover (2) in a surrounding mode, an upper Fang Waijuan of the box body (1) is clamped in the annular groove (4), buckles (22) are integrally connected to four sides of the box cover (2), and the buckles (22) are buckled on buckle plates (11) of the outer ring of the box body (1).
2. A sterilization case for plant tissue culture according to claim 1, wherein: a gasket (32) is fixedly arranged around the outer ring of the filter (3) above the screw cylinder (31), and the gasket (32) is abutted against the upper surface of the box cover (2).
3. A sterilization case for plant tissue culture according to claim 1, wherein: the inner surface of the ring groove (4) is embedded and fixed with a sealing ring (41), and the upper surface of the box body (1) is abutted against the surface of the sealing ring (41).
4. A sterilization case for plant tissue culture according to claim 1, wherein: the annular screw cover (33) is connected to the outer ring above the filter (3) in a screwing mode, and the inner part of the annular screw cover (33) is tightly pressed on the surface of the microporous filter plate (34).
5. A sterilization case for plant tissue culture according to claim 1, wherein: the inner ring of the screw hole (21) and the outer ring of the screw cylinder (31) are both provided with threads, and the screw cylinder (31) is screwed into the screw hole (21) through the threads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321084362.4U CN219878717U (en) | 2023-05-08 | 2023-05-08 | Sterilizing box for plant tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321084362.4U CN219878717U (en) | 2023-05-08 | 2023-05-08 | Sterilizing box for plant tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219878717U true CN219878717U (en) | 2023-10-24 |
Family
ID=88395559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321084362.4U Active CN219878717U (en) | 2023-05-08 | 2023-05-08 | Sterilizing box for plant tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219878717U (en) |
-
2023
- 2023-05-08 CN CN202321084362.4U patent/CN219878717U/en active Active
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