CN220056880U - Stem cell culture container - Google Patents

Abstract

The utility model provides a stem cell culture container, comprising: division board and container, the inner chamber middle part fixed connection partition board of division board, and the bleeder vent is seted up to the bottom symmetry at division board inner chamber both ends, and the one end fixed connection sterilization subassembly of gas-supply subassembly is kept away from to the division board inner chamber, and the exhaust hole is all seted up to the one end that box and division board are close to sterilization subassembly simultaneously, the culture tank is seted up to container upper surface, and the culture tank middle part is through sealed bearing swing joint axis of rotation, and axis of rotation fixed surface connection stirring leaf, and the lower extreme of axis of rotation passes through the worm wheel and connects the worm. According to the utility model, through the cooperation of the partition plate, the container box, the rotating shaft, the stirring blade and the gas transmission component, the stem cells in the culture container can be correspondingly cultured under the proper oxygen concentration, the culture effect of the stem cells is improved, and the stem cells and the culture solution can be ensured to be fully contacted, so that the stem cells can absorb the sufficient culture solution.

Description

Stem cell culture container

Technical Field

The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture container.

Background

Stem cells are a class of pluripotent cells with self-replicating capacity; under certain conditions, it can differentiate into various functional cells, and can be divided into embryonic stem cells and adult stem cells according to the development stage of stem cells; according to the developmental potential of stem cells, three categories can be distinguished: totipotent stem cells, multifunctional stem cells, and unipotent stem cells; stem cells are an insufficiently differentiated, yet immature cell with the potential function of regenerating various tissues, organs and human bodies, and are called "universal cells" in the medical community; therefore, stem cell culture is particularly important, but various problems exist in the prior art about the container for stem cell culture, such as the inability to culture stem cells with different potentials according to different requirements, slow propagation speed and easy adherent growth of stem cells.

Through retrieval, a novel mesenchymal stem cell culture container (application number: CN 201822257096.6) disclosed in the prior art is described as a plurality of culture dishes which are sequentially stacked from top to bottom; a cover body is arranged at the upper end of the culture dish positioned on the top layer of the plurality of culture dishes, and the cover body seals the culture dish; each culture dish is of a cylindrical structure, and the lower part of each culture dish is of a sealing structure; and an albumin coating layer is arranged on the side wall and the bottom of each culture dish, and the albumin coating layer is adsorbed on the side wall or the bottom of each culture dish. "; the culture container can be used for respectively culturing stem cells with different potentials by a plurality of culture dishes, so that the same culture container can be used for simultaneously culturing the stem cells with different potentials; the propagation efficiency of stem cells can be improved; the albumin coating layer can reduce the adsorption of stem cells on the inner wall and the bottom of the culture dish, which is beneficial to the development of the stem cells, but the culture container is stacked in an accumulated mode through the annular convex bodies, so that the stem cells in the culture dish are in a relatively closed environment, after the culture is carried out for a period of time, the oxygen content in the culture dish is exhausted, the stem cells can only breathe in an anaerobic mode, the culture of the stem cells is unfavorable, the culture container needs to shake the container for a long time without time in the process of culturing the stem cells, the stem cells can be promoted to fully absorb the culture solution, and a great deal of manpower and a great deal of time are wasted in a manual shaking mode.

Disclosure of Invention

In order to overcome the defects in the prior art, a stem cell culture container is provided to solve the problems in the prior art.

To achieve the above object, there is provided a stem cell culture vessel comprising: division board and container box, box lateral surface fixed connection gas-supply subassembly, and box internal fixed connection division board, container box pass through division board sliding connection in the box, the inner chamber middle part fixed connection partition board of division board, and the bleeder vent is seted up to the bottom symmetry at division board inner chamber both ends, and the one end fixed connection sterilization subassembly of gas-supply subassembly is kept away from to the division board inner chamber, and the exhaust hole is all offered to the one end that box and division board are close to sterilization subassembly simultaneously to the inner chamber of gas-supply subassembly intercommunication division board, the culture tank is offered to container box upper surface, and the culture tank middle part passes through sealed bearing swing joint axis of rotation, and axis of rotation surface fixed connection stirring leaf, and the lower extreme of axis of rotation passes through worm wheel connection worm, and the worm passes through bearing swing joint in the drive tank that the container box was offered, and the one end fixed connection driven plate of worm simultaneously, driven plate connection initiative dish, and initiative dish pass through the bevel gear connection transmission shaft, the transmission shaft is through the recess that the side of bearing swing joint box was offered in, the bottom fixed connection main motor of recess, main motor passes through the lower extreme of coupling joint transmission shaft.

Preferably, the division board is rectangle structure, equidistant fixed connection three component baffles in the box, and the cross-section of division board is the mouth font structure, and division board fixed connection's in the middle part of the division board is rectangle structure simultaneously, and the cross-section that division board and division board combination constitute together is the day font structure.

Preferably, the two ends of the lower surface of the partition plate are respectively provided with a plurality of groups of air holes at equal intervals, one end of the air hole close to the box body is inclined downwards, and the position of the surface of the box body opposite to the opening of the air hole is fixedly connected with the dust screen.

Preferably, the sterilization assembly comprises a frame body, baffles and ultraviolet lamps, the frame body is in a shape like a Chinese character 'kou', two groups of baffles are symmetrically connected to two sides of the bottom of the frame body, a group of baffles are fixedly connected to the middle part of the top of the frame body, the ultraviolet lamps are fixedly connected to the middle part of the bottom of the frame body, and the whole cross section of the sterilization assembly is in a U-shaped structure.

Preferably, the whole rectangle structure that is of container box, the culture tank that the container box upper surface was seted up is cylindrical structure, and the opening size looks adaptation that container box and box side were seted up, and the transmission groove of seting up in the container box is L shape structure simultaneously.

Preferably, the driving disc is movably connected with the inner side surface of the box body in parallel at equal intervals through a bearing, the shaft section of the driving disc is of a T-shaped structure, the driven disc fixedly connected with the worm is of a circular structure, and the surfaces of the driven disc and the driving disc, which are opposite, are fixedly connected with the friction layer.

Preferably, the stirring blade comprises a molding plate and a buffer layer, the buffer layer is made of silica gel, a buffer groove is formed in one end, close to the gas transmission assembly, of the partition plate, the buffer groove penetrates through the lower surface of the partition plate, the embedded plate is connected in the buffer groove in a sliding mode through a spring, meanwhile, the cross section of the embedded plate is of a convex structure, and the embedded groove is correspondingly formed in the position, corresponding to the opening of the buffer groove, of the upper surface of the container box.

Compared with the prior art, the utility model has the beneficial effects that: through division board, bleeder vent, partition panel, gas-supply subassembly, sterilization subassembly and the cooperation of exhaust hole for the stem cell in this container box can carry out corresponding cultivation under suitable oxygen concentration, improves stem cell culture's effect, and the embedded board can imbed the gomphosis inslot of corresponding container box seting up under the effect of the inside atmospheric pressure of division board moreover, thereby strengthen the fixed effect between box and the container box, avoid the container box unexpected to open, simultaneously, through axis of rotation, stirring leaf, worm, driven plate, initiative dish, transmission shaft and main motor's cooperation, make the stirring leaf can carry out continuous low-speed rotation in the culture tank, can ensure then that stem cell fully absorbs the culture solution, thereby practice thrift corresponding manpower and time.

Drawings

Fig. 1 is a schematic front view of an embodiment of the present utility model.

Fig. 2 is a schematic top view of an embodiment of the present utility model.

Fig. 3 is a schematic top view of a separator plate according to an embodiment of the present utility model.

Fig. 4 is an enlarged schematic view at a of fig. 1 according to an embodiment of the present utility model.

In the figure: 1. a case; 2. a partition plate; 3. a container box; 4. a sterilization assembly; 5. a rotating shaft; 6. stirring the leaves; 7. a worm; 8. a partition panel; 9. a main motor; 10. a transmission shaft; 11. an embedded plate; 12. a gas delivery assembly; 13. a driving disk; 14. a driven plate; 15. and a fitting groove.

Detailed Description

Other advantages and effects of the present utility model will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present utility model with reference to specific examples. The utility model may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present utility model.

Referring to fig. 1 to 4, the present utility model provides a stem cell culture container comprising: division plate 2 and container box 3, 1 lateral surface fixed connection gas-supply subassembly 12 of box, and 1 internal fixation division plate 2 of box, container box 3 pass through division plate 2 sliding connection in box 1, the inner chamber middle part fixed connection partition plate 8 of division plate 2, and the bleeder vent is seted up to the bottom symmetry at division plate 2 inner chamber both ends, and the one end fixed connection sterilization subassembly 4 of 2 inner chambers of division plate keep away from gas-supply subassembly 12, the exhaust hole is all offered to the one end that the sterilization subassembly 4 was close to simultaneously box 1 and division plate 2, and the inner chamber of gas-supply subassembly 12 intercommunication division plate 2, the culture tank is offered to container box 3 upper surface, and the culture tank middle part passes through sealed bearing swing joint axis of rotation 5, and axis of rotation 5 fixed connection stirring leaf 6, and the lower extreme of axis of rotation 5 passes through worm wheel connection worm 7, worm 7 passes through the drive tank that bearing swing joint offered at container box 3, simultaneously worm 7's one end fixed connection driven disc 14, driven disc 13 passes through bearing swing joint at 1 medial surface of box, and driving disc 13 passes through helical gear connection transmission shaft 10, transmission shaft 10 passes through the recess motor fixed connection 9 of the bottom that the motor of bearing swing joint box 1 side was offered, bottom of motor shaft 9 is passed through the main shaft coupling 10.

In this embodiment, different stem cells to be cultured are respectively placed in culture tanks provided with corresponding container boxes 3, when the container boxes 3 are embedded into the box 1 through openings provided with the box 1, a sterile blower (not shown in the figure) in the gas transmission assembly 12 is started at the same time, so that sterile oxygen flows into inner cavities of the corresponding partition plates 2 through gas transmission pipes in the gas transmission assembly 12 respectively, then flows into the culture tanks from air holes on one side of the partition plates 2, flows into the inner cavities of the partition plates 2 from air holes on the other side of the partition plates 2, ensures that the oxygen content in the culture tanks is in a proper range, and starts the switch of the ultraviolet lamp, so that the air flow can perform corresponding sterilization treatment when flowing through the sterilization assembly 4, thereby avoid the external air to pollute the culture tank through the exhaust hole, and last from exhaust hole in-and-out air current, can avoid the problem that the culture tank was polluted, and at the in-process that division board 2 inner chamber atmospheric pressure increased, the atmospheric pressure can promote embedded plate 11 and move down, then embedded plate 11 can imbed in the embedded groove 15 that container box 3 offered, accomplish fixed connection between box 1 and the container box 3, afterwards, start the switch of main motor 9, main motor 9 passes through the shaft coupling and drives transmission shaft 10 and rotate, and transmission shaft 10 passes through helical gear and initiative dish 13 and drives corresponding driven plate 14 and worm 7 synchronous rotation, make worm 7 can drive corresponding axis of rotation 5 and stirring leaf 6 through the worm wheel of meshing and rotate, ensure that stem cell can fully absorb the culture solution.

As a preferred embodiment, the partition plate 2 is in a rectangular structure, three groups of partition plates 2 are fixedly connected in the box body 1 at equal intervals, the cross section of the partition plate 2 is in a square structure, meanwhile, the partition plate 8 fixedly connected in the middle of the partition plate 2 is in a rectangular structure, and the cross section formed by combining the partition plate 2 and the partition plate 8 is in a Chinese character 'ri' shape.

In this embodiment, like fig. 1 and 3, setting up of division board 2 for the inner chamber of box 1 is equally divided into three, then can be convenient for the embedding of container box 3, and container box 3 and box 1 stabilize inner chamber size looks adaptation moreover, avoid container box 3 to appear rocking the problem, and the setting up of partition panel 8 simultaneously then can ensure that the air current flows through the culture tank.

As a preferred embodiment, two ends of the lower surface of the partition plate 2 are respectively provided with a plurality of groups of air holes at equal intervals in parallel, one end of the air hole close to the box body 1 is inclined downwards in an inclined way, and the surface of the box body 1 is fixedly connected with a dust screen at the position opposite to the opening of the air hole.

In this embodiment, as shown in fig. 1 and 3, the ventilation holes are formed, so that the sterile air flow can flow in from one end of the partition plate 2, and then flows out from the other end of the partition plate 2 after flowing through the culture tank, so that the oxygen content in the culture tank can be ensured to be in a proper range, and meanwhile, the probability that external bacteria flow into the partition plate 2 can be reduced due to the ventilation holes with oblique structures.

As a preferred embodiment, the sterilization assembly 4 is composed of a frame body, baffles and ultraviolet lamps, the frame body is in a shape like a Chinese character 'kou', two groups of baffles are symmetrically connected to two sides of the bottom of the frame body, a group of baffles are fixedly connected to the middle part of the top of the frame body, the ultraviolet lamps are fixedly connected to the middle part of the bottom of the frame body, and the whole cross section of the sterilization assembly 4 is in a U-shaped structure.

In this embodiment, as shown in fig. 1 and 3, the positions of the three groups of baffles are distributed, so that the flow path of the air flow in the frame body can be prolonged, the ultraviolet lamp is ensured to sufficiently sterilize the air flow flowing through, and the curved inner structure of the sterilization assembly 4 can also effectively intercept dust or bacteria accidentally flowing from the outside, so that the culture tank is prevented from being polluted.

As a preferred embodiment, the whole container box 3 is in a rectangular structure, the culture tank arranged on the upper surface of the container box 3 is in a cylindrical structure, the sizes of the openings arranged on the side surfaces of the container box 3 and the box body 1 are matched, and meanwhile, the transmission tank arranged in the container box 3 is in an L-shaped structure.

In this embodiment, as shown in fig. 1 and 4, the cylindrical culture tank provided in the container box 3 is convenient for the rotation of the stirring blade 6, so that when the stirring blade 6 rotates, the culture solution and the stem cells can be stirred to the greatest extent, the stem cells can be ensured to fully absorb the culture solution, and the stability of the container box 3 during embedding or taking out can be enhanced due to the adaptation of the sizes of the openings provided in the side surfaces of the container box 3 and the box body 1.

As a preferred implementation mode, the driving disc 13 is movably connected with the inner side surface of the box body 1 at equal intervals in parallel through bearings, the shaft section of the driving disc 13 is of a T-shaped structure, the driven disc 14 fixedly connected with the worm 7 is of a circular structure, and the opposite surfaces of the driven disc 14 and the driving disc 13 are fixedly connected with the friction layer.

In this embodiment, as shown in fig. 4, the friction layer is made of medical silica gel, and the surface of the friction layer is uniformly provided with anti-slip stripes, so that stable synchronous rotation can be performed when the driven disc 14 and the driving disc 13 are tightly attached, and accordingly, the stirring blade 6 can be ensured to rotate correspondingly in the culture tank.

As a preferred embodiment, stirring vane 6 comprises shaping board and buffer layer, and the buffer layer adopts the silica gel material, and just is close to the one end of gas-supply subassembly 12 in the division board 2 and offer the dashpot, and the dashpot runs through the lower surface of division board 2, and embedding board 11 passes through spring sliding connection in the dashpot, and embedding board 11 cross-section is protruding font structure simultaneously, and the embedding groove 15 is offered to the relative dashpot open-ended position correspondence of container box 3 upper surface.

In this embodiment, as shown in fig. 2, fig. 3 and fig. 4, the buffer layer is made of medical silica gel, which can avoid adverse effects on stem cells when the stirring blade 6 rotates at a low speed, so as to ensure that the stem cells can fully absorb nutrient solution, and meanwhile, the box body 1 can be fixedly connected with the container box 3 correspondingly, and when a certain container box 3 needs to be taken out independently, only a pipeline is needed to correspond to the valve of the air pipe communicated with the partition plate 2, so that the air pressure in the corresponding partition plate 2 is recovered to be normal, and then the embedding plate 11 can be reset under the action of the compressed spring, so that the embedding plate 11 can be separated from the embedding groove 15 corresponding to the container box 3, and a incubator can take out the corresponding container box 3 conveniently.

According to the stem cell culture container, through the cooperation of the partition plate 2, the container box 3, the rotating shaft 5, the stirring blade 6 and the gas transmission assembly 12, stem cells in the culture container can be correspondingly cultured under a proper oxygen concentration, the culture effect of the stem cells is improved, the stem cells can be ensured to be fully contacted with culture solution, and the stem cells can absorb enough culture solution.

Claims (7)

1. A stem cell culture vessel comprising: division board (2) and container box (3), box (1) lateral surface fixed connection gas-supply subassembly (12), and fixed connection division board (2) in box (1), container box (3) pass through division board (2) sliding connection in box (1), its characterized in that: the utility model discloses a novel sterilization device, which is characterized in that a partition plate (2) is fixedly connected with a partition plate (8) in the middle of an inner cavity, ventilation holes are symmetrically formed in the bottoms of two ends of the inner cavity of the partition plate (2), one end of the inner cavity of the partition plate (2) away from a gas transmission component (12) is fixedly connected with a sterilization component (4), simultaneously, one ends of a box body (1) and the partition plate (2) close to the sterilization component (4) are provided with exhaust holes, the gas transmission component (12) is communicated with the inner cavity of the partition plate (2), a culture tank is formed in the upper surface of a container box (3), the middle of the culture tank is movably connected with a rotating shaft (5) through a sealing bearing, the surface of the rotating shaft (5) is fixedly connected with a stirring blade (6), the lower end of the rotating shaft (5) is connected with a worm wheel through a worm wheel, the worm wheel (7) is movably connected with a driving disk (14) through a bearing in a transmission tank formed in the container box (3), one end of the worm wheel (7) is fixedly connected with a driving disk (13), the driving disk (13) is connected with the driving disk (10) through a bearing and is movably connected with the inner side surface of the box body (1), the driving disk (13) is connected with the inner side surface of the inner side of the box body through a bearing through a bevel wheel, the bevel wheel is fixedly connected with the bottom of the groove (9) formed in the side surface of the side of the motor, the main motor (9) is connected with the lower end of the transmission shaft (10) through a coupler.

2. The stem cell culture container according to claim 1, wherein the partition plate (2) has a rectangular structure, the three-component partition plates (2) are fixedly connected in the box body (1) at equal intervals, the cross section of the partition plate (2) has a square structure, the partition plate (8) fixedly connected in the middle of the partition plate (2) has a rectangular structure, and the cross section formed by combining the partition plate (2) and the partition plate (8) has a Chinese character 'ri'.

3. The stem cell culture container according to claim 1, wherein a plurality of groups of ventilation holes are formed in parallel and equidistant at two ends of the lower surface of the partition plate (2), one end of the ventilation hole, which is close to the box body (1), is inclined obliquely downwards, and the surface of the box body (1) is fixedly connected with a dust screen at a position opposite to the opening of the ventilation hole.

4. The stem cell culture container according to claim 1, wherein the sterilizing unit (4) is composed of a frame body, baffles and ultraviolet lamps, the frame body is in a shape of a Chinese character 'kou', two groups of baffles are symmetrically connected to two sides of the bottom of the frame body, a group of baffles are fixedly connected to the middle part of the top of the frame body, the ultraviolet lamps are fixedly connected to the middle part of the bottom of the frame body, and the whole cross section of the sterilizing unit (4) is in a U-shaped structure.

5. The stem cell culture container according to claim 1, wherein the container box (3) is of a rectangular structure as a whole, the culture tank formed on the upper surface of the container box (3) is of a cylindrical structure, the size of the opening formed on the side surface of the container box (3) and the side surface of the box body (1) are matched, and meanwhile, the transmission tank formed in the container box (3) is of an L-shaped structure.

6. The stem cell culture container according to claim 1, wherein the driving disc (13) is movably connected with the inner side surface of the box body (1) at equal intervals in parallel through bearings, the shaft section of the driving disc (13) is of a T-shaped structure, the driven disc (14) fixedly connected with the worm (7) is of a circular structure, and the opposite surfaces of the driven disc (14) and the driving disc (13) are fixedly connected with the friction layer.

7. The stem cell culture container according to claim 1, wherein the stirring blade (6) is composed of a molding plate and a buffer layer, the buffer layer is made of silica gel, a buffer groove is formed in one end, close to the gas transmission component (12), of the partition plate (2), the buffer groove penetrates through the lower surface of the partition plate (2), the embedded plate (11) is slidably connected in the buffer groove through a spring, meanwhile, the cross section of the embedded plate (11) is of a convex structure, and the embedded groove (15) is correspondingly formed in the position, corresponding to the opening of the buffer groove, of the upper surface of the container box (3).