CN219342174U

CN219342174U - Stem cell preparation proliferation incubator

Info

Publication number: CN219342174U
Application number: CN202320433274.4U
Authority: CN
Inventors: 刘招; 米楠; 刘芳
Original assignee: Wuhan Wanhai Cell Biotechnology Co ltd
Current assignee: Wuhan Wanhai Cell Biotechnology Co ltd
Priority date: 2023-03-09
Filing date: 2023-03-09
Publication date: 2023-07-14
Anticipated expiration: 2033-03-09

Abstract

The utility model discloses a stem cell preparation proliferation incubator, which comprises a base, wherein a placing groove is formed in the middle of the top of the base, a heater is arranged at the bottom of the inner side of the placing groove, a culture dish is arranged at the inner side of the placing groove, electric push rods are arranged at the two sides of the top of the base, mounting boxes are commonly arranged at the output ends of the two groups of electric push rods, a motor is arranged in the middle of the top of the inner side of the mounting box, and a rotating rod is arranged at the output end of the motor; according to the utility model, through the mutual matching among the motor, the spring A, the sliding groove, the cam, the rotating rod, the sliding block, the cover plate and the stirring blade, when the mounting box is controlled to be closed for proliferation culture of cells, the stirring blade can be driven to extend into the culture dish, the stirring blade can be controlled to rotate subsequently for stirring and mixing the culture solution, the uniform reaction of the culture solution is ensured, the cover plate can be driven to be tightly buckled at the top of the culture dish after the cover plate is closed, the culture dish is pressed and fixed, and the stability of the culture dish is improved.

Description

Stem cell preparation proliferation incubator

Technical Field

The utility model relates to the technical field of stem cell preparation, in particular to a stem cell preparation proliferation incubator.

Background

The stem cells are multipotent cells with self-replication capacity, can be differentiated into a plurality of functional cells under certain conditions, are underdifferentiated and immature cells, have the potential functions of regenerating various tissues and organs and human bodies, are called universal cells in the medical field, and are required to be cultured in a proliferation incubator when being prepared for proliferation culture so as to ensure the proliferation effect of the cells;

the patent with the prior authority bulletin number of CN 212025372U discloses a stem cell incubator, which comprises a box body, a sealing cover, a culture dish, a pull rod and a sterilizing device. The upper end of the box body is provided with an opening, the sealing cover can seal the opening at the upper end of the box body, and the upper part of the inner wall of the box body is convexly provided with a plurality of guide plates, so that the operation is convenient, a relatively closed environment is created, the carbon dioxide concentration in the environment is ensured, and meanwhile, the gas circulated in the environment can be sterilized;

however, the following disadvantages exist in this device:

1. in the device, when the cells are cultured, the culture solution is difficult to mix and stir with the cells, so that uneven mixing of the culture solution is easy to occur, and the proliferation of the cells is influenced;

2. when the cell culture is carried out, the device is required to be ventilated, and dust and bacteria are usually carried in ventilated gas, so that the dust can enter the device to pollute the culture solution when the gas enters the device, and the cell survival is affected.

Disclosure of Invention

The present utility model is directed to a stem cell preparation proliferation incubator, which solves the above-mentioned problems of the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the stem cell preparation proliferation incubator comprises a base, wherein a placing groove is formed in the middle of the top of the base, a heater is installed at the bottom of the inner side of the placing groove, a culture dish is arranged on the inner side of the placing groove, electric push rods are arranged on two sides of the top of the base, an installation box is arranged at the output ends of the electric push rods together, a motor is installed at the middle of the top of the inner side of the installation box, a rotating rod is arranged at the output end of the motor, stirring blades are arranged at the bottom of the rotating rod, sliding grooves are formed in the bottoms of two sides of the inner side of the installation box, sliding blocks are connected to the inner sides of the sliding grooves in a sliding mode, springs A are arranged at the tops of the two sliding blocks, a cover plate is arranged on one side, close to the other side of the sliding blocks, and the cover plate is sleeved on the outer side of the rotating rod;

the top of the inboard one side of installation box is provided with the air pump, the top of installation box one side is provided with the active carbon purification box, the middle part of installation box one side is provided with the intake pipe that is linked together with the active carbon purification box, the input of air pump is provided with the connecting pipe that is linked together with the active carbon purification box, the inboard middle part of intake pipe is provided with the filter screen, the middle part cover of the inboard one side of installation box is equipped with the push rod, one side of push rod extends to the inboard of intake pipe and is provided with the brush, the outside cover of push rod is equipped with spring B, the middle part in the bull stick outside is provided with the cam with push rod matched with.

Preferably, the cover plate is matched with the rotating rod in a sliding connection mode, a vent is formed in one side of the top of the cover plate, the cover plate can slide along the outer side of the rotating rod, and gas can enter the culture dish through the vent.

Preferably, the bottom of one side of the inner side of the air inlet pipe is provided with a storage groove, and the hairbrush is positioned at the inner side of the storage groove and used for storing the hairbrush.

Preferably, a limiting block is arranged on one side of the outer side of the push rod, the diameter of the limiting block is larger than that of the spring B, the spring B can be limited, and when the push rod moves to one side, the spring B can be pushed to compress.

Preferably, an exhaust pipe is arranged at the output end of the air pump and used for guiding the air.

Preferably, the both sides at base top are provided with the mounting groove, and two sets of electric putter is located the mounting groove inboard, provides installation space for electric putter, reduces the installation volume.

Compared with the prior art, the utility model has the beneficial effects that: preparing a proliferation incubator by the stem cells;

1. through the mutual cooperation among the motor, the spring A, the sliding groove, the cam, the rotating rod, the sliding block, the cover plate and the stirring blade, when the device controls the mounting box to be closed for proliferation culture of cells, the stirring blade can be driven to extend into the culture dish, the follow-up stirring blade can be controlled to rotate for stirring and mixing the culture solution, the uniform reaction of the culture solution is ensured, the cover plate can be driven to be tightly buckled at the top of the culture dish after the cover plate is closed, the culture dish is pressed and fixed, the stability of the culture dish is improved, the culture dish can be prevented from shaking when the culture solution is stirred, and the fixation stability is improved;

2. through mutually supporting between active carbon purification box, intake pipe, push rod, air pump, connecting pipe, brush and the spring B for when ventilating in the cultivation process, can filter gas through the filter screen earlier, the rethread active carbon purifies the box and purifies gas, in order to prevent that dust bacterium from getting into the device and causing the pollution to cultivateing the cell, and when the stirring of device control stirring leaf to the culture solution, but also intermittent type formula promote the brush and brush the cam, clear away its filter dust, can prevent that it from taking place to block up, promoted stability in use.

Drawings

FIG. 1 is a perspective view of the present utility model;

FIG. 2 is a front cross-sectional view of the present utility model;

FIG. 3 is a front cross-sectional view of the mounting case of the present utility model;

fig. 4 is an enlarged schematic view of the structure of fig. 3 a according to the present utility model.

In the figure: 1. a base; 2. a heater; 3. a placement groove; 4. an electric push rod; 5. a culture dish; 6. a mounting box; 7. a motor; 8. an activated carbon purification box; 9. an air inlet pipe; 10. a spring A; 11. a chute; 12. a cam; 13. a rotating rod; 14. a push rod; 15. an air pump; 16. a connecting pipe; 17. a slide block; 18. a cover plate; 19. stirring the leaves; 20. a brush; 21. a filter screen; 22. and a spring B.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-4, embodiments of the present utility model are provided: the utility model provides a stem cell preparation proliferation incubator, including base 1, the middle part at base 1 top is provided with standing groove 3, heater 2 is installed to the bottom of standing groove 3 inboard, the inboard of standing groove 3 is provided with culture dish 5, the both sides at base 1 top are provided with electric putter 4, the both sides at base 1 top are provided with the mounting groove, two sets of electric putter 4 are located the mounting groove inboard, provide installation space for electric putter 4, reduce the installation volume, the output of two sets of electric putter 4 is provided with mounting box 6 jointly, the mid-mounting at the inboard top of mounting box 6 has motor 7, the output of motor 7 is provided with bull stick 13, the bottom of bull stick 13 is provided with stirring leaf 19, spout 11 has been seted up to the bottom of the inboard both sides of mounting box 6, the inboard sliding connection of two sets of spout 11 has slider 17, the top of two sets of slider 17 is provided with spring A10, two sets of slider 17 are close to one side jointly be provided with apron 18, and apron 18 cover the outside with bull stick 13, apron 18 is in sliding connection with bull stick 13, one side at 18 top for apron 18 top can slide along the outside of bull stick 13, can get into in the vent 5 through the vent opening of gas;

the top of the inboard side of mounting box 6 is provided with air pump 15, the output of air pump 15 is provided with the blast pipe, be used for carrying out the water conservancy diversion to gas, the top of mounting box 6 one side is provided with active carbon purification box 8, the middle part of mounting box 6 one side is provided with the intake pipe 9 that is linked together with active carbon purification box 8, the storage tank has been seted up to the bottom of the inboard side of intake pipe 9, brush 20 is located the storage tank inboard, be used for accomodating brush 20, the input of air pump 15 is provided with the connecting pipe 16 that is linked together with active carbon purification box 8, the inboard middle part of intake pipe 9 is provided with filter screen 21, the middle part cover of the inboard side of mounting box 6 is equipped with push rod 14, one side of push rod 14 extends to the inboard of intake pipe 9 and is provided with brush 20, the outside cover of push rod 14 is equipped with spring B22, one side of push rod 14 outside is provided with the stopper, and the diameter of stopper is greater than spring B22, can spacing spring B22, when push rod 14 moves to one side, the middle part outside can promote spring B22 compression, the push rod 13 is provided with push rod 14 matched with cam 12.

Working principle: when the device is used, a culture dish 5 for culturing stem cells can be placed in a placing groove 3, then two groups of electric push rods 4 are started, a mounting box 6 can be driven to move downwards and be clamped at the outer side of the placing groove 3 by the two groups of electric push rods 4, stirring blades 19 can extend into the mounting box 6 when the mounting box 6 moves downwards, a cover plate 18 can be clamped at the top of the culture dish 5, in the process of moving downwards the mounting box 6, the elastic force of a spring A10 is overcome through the guiding of a sliding block 17, the cover plate 18 can be pushed to be tightly buckled at the top of the culture dish 5 after the mounting box 6 is clamped at the outer side of the placing groove 3 by utilizing the elastic restoring force of the spring A10, the culture dish 5 is pressed and fixed, the stability of the culture dish 5 is improved, then a motor 7 is started, a rotating rod 13 can be driven to be selected by the motor 7, then the rotating rod 13 is driven to rotate, the stirring blades 19 can be used for stirring and mixing stem cells in the culture dish 5 and culture solution can be stirred uniformly by the stirring blades 19, meanwhile the cover plate 18 can be prevented from scattering the culture solution when the culture solution is stirred, and the culture dish 5 is prevented from shaking by the cover plate 18, and the stability of the culture dish 5 is prevented from shaking;

in the culture process, the device needs to be ventilated to ensure survival of cells, when the device is ventilated, the air pump 15 can be started to extract air, the air can firstly enter the air inlet pipe 9, then the air can be filtered through the filter screen 21 to remove dust therein, after the air is filtered, the air can be further enter the activated carbon purification box 8 to be purified, then the air can enter the installation box 6 to be ventilated through the diversion of the connecting pipe 16, when the device controls the stirring blade 19 to stir culture solution, the cam 12 can be driven to rotate through the rotating rod 13, then the push rod 14 can be intermittently pushed by the cam 12 to overcome the elasticity of the spring B22 to move to one side, so that the push rod 14 can drive the brush 20 to brush the cam 12 to remove filtered dust, the blockage can be prevented, the push rod 14 can be pushed to reset through the elastic restoring force of the spring B22, and the filter screen 21 can be repeatedly cleaned.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front," "rear," "head," "tail," and the like are used as an orientation or positional relationship based on that shown in the drawings, merely to facilitate description of the utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the utility model. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "connected" and "connected" are to be construed broadly, and may be either a fixed connection or a removable connection, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Claims

1. A stem cell preparation proliferation incubator, which comprises a base (1), and is characterized in that: the middle part at the top of the base (1) is provided with a placing groove (3), the bottom at the inner side of the placing groove (3) is provided with a heater (2), the inner sides of the placing groove (3) are provided with a culture dish (5), the two sides at the top of the base (1) are provided with electric push rods (4), the output ends of the two groups of electric push rods (4) are jointly provided with a mounting box (6), the middle part at the inner side top of the mounting box (6) is provided with a motor (7), the output end of the motor (7) is provided with a rotating rod (13), the bottom of the rotating rod (13) is provided with a stirring blade (19), the bottoms at the two sides at the inner side of the mounting box (6) are provided with sliding grooves (11), the tops of the two groups of the sliding grooves (11) are provided with a spring A (10), the two groups of the sliding grooves (17) are mutually close to one side and are jointly provided with a cover plate (18), and the cover plate (18) is sleeved on the outer side of the rotating rod (13);

the utility model discloses a novel air conditioner, including installation box (6), brush, push rod (14), spring B (22) are provided in the middle part of installation box (6), air pump (15) are provided with air pump (15) at the top of inboard one side of installation box (6), the top of installation box (6) one side is provided with active carbon purification box (8), intake pipe (9) that are linked together with active carbon purification box (8) are provided with input of air pump (15), the inboard middle part of intake pipe (9) is provided with filter screen (21), the middle part cover of installation box (6) inboard one side is equipped with push rod (14), one side of push rod (14) extends to the inboard of intake pipe (9) and is provided with brush (20), the outside cover of push rod (14) is equipped with spring B (22), the middle part in the outside of bull stick (13) is provided with cam (12) with push rod (14) matched with.

2. The stem cell preparation proliferation incubator according to claim 1, wherein: the cover plate (18) is matched with the rotating rod (13) in a sliding connection mode, and a ventilation opening is formed in one side of the top of the cover plate (18).

3. The stem cell preparation proliferation incubator according to claim 1, wherein: the bottom of one side of the inner side of the air inlet pipe (9) is provided with a storage groove, and the hairbrush (20) is positioned at the inner side of the storage groove.

4. The stem cell preparation proliferation incubator according to claim 1, wherein: a limiting block is arranged on one side of the outer side of the push rod (14), and the diameter of the limiting block is larger than that of the spring B (22).

5. The stem cell preparation proliferation incubator according to claim 1, wherein: an exhaust pipe is arranged at the output end of the air pump (15).

6. The stem cell preparation proliferation incubator according to claim 1, wherein: the two sides of the top of the base (1) are provided with mounting grooves, and the two groups of electric push rods (4) are positioned on the inner sides of the mounting grooves.