CN216663102U - Microorganism culture and quantitative liquid taking device - Google Patents

Abstract

The utility model provides a microbial cultivation and quantitative liquid taking device, which comprises a box body, wherein the box body sequentially comprises a cultivation chamber, a collection chamber and a liquid taking area from top to bottom; the culture chamber and the collection chamber are separated by a heat insulation plate; collect the room and openly be equipped with the room door that can open and shut, be equipped with the collection cup in the collection room, the top one side of collecting the cup is equipped with the first drain pipe that extends out from cultivateing the room, and the second drain pipe is installed to the opposite side, is equipped with flow rate controller on the second drain pipe, and second drain pipe lower extreme is for getting the liquid district. The beneficial effect of this application lies in: firstly, the microorganism is cultured in an independent culture dish and isolated from the external environment, the culture dish does not need to be opened during sampling, the growth environment of the microorganism is not interfered, and the probability of liquid taking pollution in the microorganism culture process is reduced; the microbial culture and liquid taking are carried out simultaneously, so that the condition that thalli are not uniformly distributed is prevented; thirdly, can realize the ration sample, easy operation, convenient and fast reduces the manpower consumption.

Description

Microorganism culture and quantitative liquid taking device

Technical Field

The utility model belongs to the technical field of microbial culture, and relates to a microbial culture and quantitative liquid taking device.

Background

The microbial culture refers to the rapid growth and propagation of some (kinds of) microorganisms by means of artificially prepared culture media and artificially created culture conditions (such as culture temperature, and the like), and is called microbial culture. Microorganisms are usually inoculated into a culture dish containing a volume of medium and placed in a culture apparatus for culturing.

However, when a microorganism experiment is performed, in the process of multiple sampling, the situation that the microorganism is polluted inevitably occurs, and when the bacteria liquid is inoculated, the culture dish needs to be taken down from the shaking table, and a certain time interval exists due to manual operation, so that part of bacteria is precipitated at the bottom of the culture dish, the distribution of the bacteria in the original bacteria liquid is uneven, the difference of the bacteria amount added into the sample is very large, and the experimental data detected after the sample is cultured for a certain period has great influence, and great errors are caused.

SUMMERY OF THE UTILITY MODEL

In order to reduce the possibility of contamination of the bacterial liquid and improve the liquid taking efficiency, the utility model provides a microbial culture and quantitative liquid taking device, so that the strain culture and the bacterial liquid collection are carried out simultaneously, and the device is efficient and convenient.

In order to achieve the purpose, the utility model adopts the technical scheme that:

the microbial culture and quantitative liquid taking device comprises a box body, wherein the box body sequentially comprises a culture chamber, a collection chamber and a liquid taking area from top to bottom;

the front side of the culture chamber is provided with a box door capable of being opened and closed; a transparent culture dish with a base is arranged in the culture chamber, a stirrer is fixed at the bottom of the culture dish, a first liquid inlet pipe and a first liquid outlet pipe are arranged above the culture dish, one end of the first liquid inlet pipe extends out of the top of the box body, and one end of the first liquid outlet pipe is introduced into the collection chamber; an air pipe and a water pipe are respectively arranged on two sides of the culture dish; a thermometer, a hygrometer and an ultraviolet lamp are also arranged in the culture chamber; the culture chamber and the collection chamber are separated by a heat insulation plate;

collect the room and openly be equipped with the room door that can open and shut, be equipped with the collection cup in the collection room, the top one side of collecting the cup is equipped with the first drain pipe that extends out from cultivateing the room, and the second drain pipe is installed to the opposite side, is equipped with flow rate controller on the second drain pipe, and the liquid district is got in the correspondence of second drain pipe lower extreme.

Preferably, a temperature and humidity display screen is arranged at the upper right corner of the box door, and an observation screen is embedded in the center of the box door.

Preferably, the thermometer and hygrometer are fixed to the rear wall of the incubation chamber, and the ultraviolet lamp is installed on the top of the incubation chamber.

Preferably, a power switch is arranged on the right side of the box body, and an ultraviolet lamp switch is arranged at the top of the box body.

Preferably, a cup seat connected with a motor is installed in the culture chamber, and the culture dish is placed on the cup seat.

Preferably, a valve is arranged on a pipeline of the first liquid inlet pipe positioned outside the tank body.

Preferably, the air pipe is connected with an air pump, the air pump is also connected with an air inlet pipe, and a microorganism filtering device is arranged between the air inlet pipe and the air pump; the water pipe is connected with a water pump, and the water pump is connected with a water tank through a water inlet pipe.

Preferably, the right side of the front surface of the culture chamber is provided with a temperature adjusting knob, a humidity adjusting knob, a stirrer switch and an air inlet switch.

Preferably, a first liquid outlet control switch is arranged between the culture chamber and the collection chamber, a second liquid outlet control switch is arranged between the collection chamber and the liquid taking area, and a flow rate control screen is arranged on the right side of the liquid taking area.

Preferably, the liquid taking area is provided with an inwards concave rectangular opening.

The beneficial effect of this application lies in: firstly, the microorganism is cultured in an independent culture dish and isolated from the external environment, the culture dish does not need to be opened during sampling, the growth environment of the microorganism is not interfered, and the probability of liquid taking pollution in the microorganism culture process is reduced; the microorganism culture and the liquid taking are carried out simultaneously, so that the situation that in the process of sampling for a plurality of times, a culture dish leaves a shaking table, thalli are deposited at the bottom and are unevenly distributed in the culture solution, and a large error occurs in repeated experiments is prevented; thirdly, can realize the ration sample, get the liquid district for sunken rectangle, reduce and get the liquid and directly expose the miscellaneous fungus pollution risk that external environment brought, easy operation, convenient and fast reduces the manpower consumption.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a view showing the internal principal structure of the present invention;

wherein:

1-box body, 2-box door, 3-humiture display screen, 4-observation screen, 5-thermometer, 6-hygrometer, 7-heating block, 8-motor, 9-cup seat, 10-culture dish, 11-stirrer, 12-first liquid inlet pipe, 13-ultraviolet lamp switch, 14-ultraviolet lamp, 15-valve, 16-first liquid outlet pipe, 17-air pipe, 18-water pipe, 19-air pump, 20-water pump, 21-air inlet pipe, 22-microorganism filtering device, 23-water inlet pipe, 24-water tank, 25-thermal insulation board, 26-box door, 27-collection cup, 28-second liquid outlet pipe, 29-flow rate controller, 30-liquid taking area, 31-power switch, 32-temperature adjusting knob, etc, 33-humidity adjusting knob, 34-first liquid outlet control switch, 35-second liquid outlet control switch, 36-flow rate control screen, 37-stirrer switch and 38-air inlet switch.

Detailed Description

In order to more clearly illustrate the present invention, the present invention will be described in further detail below with reference to examples and the accompanying drawings. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the utility model.

In the description of the present invention, it should be noted that the terms "upper end", "lower end", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, and integral connections; they may be directly connected to each other, indirectly connected to each other through an intermediate member, or connected to each other through the inside of two members. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In addition, if no special description is given, all the components used in the following embodiments are existing components, and the corresponding connection manner thereof can also be implemented by conventional technical means, and will not be described in detail in this application.

The utility model discloses a microbial cultivation and quantitative liquid taking device, which comprises a box body 1, wherein the box body 1 sequentially comprises a cultivation chamber, a collection chamber and a liquid taking area from top to bottom, wherein:

the front of the culture room is provided with an opening, one side of the opening is hinged with a box door 2, the upper right corner of the box door 2 is fixed with a temperature and humidity display screen 3, the center of the center is embedded with an observation screen 4, and the strain culture condition can be observed through the observation screen. The rear wall of the culture chamber is fixed with a thermometer 5 and a hygrometer 6, and the top of the culture chamber is provided with an ultraviolet lamp 14. Install the cup 9 that links to each other with motor 8 in the culture chamber, the transparent culture dish 10 of subsidiary base is put to cup 9 overhead, and culture dish 10 bottom is fixed with agitator 11, and culture dish 10 upper end is equipped with first feed liquor pipe 12, and first feed liquor pipe 12 is located and is equipped with valve 15 on the pipeline of 1 outsides of box, and the right side is equipped with the first drain pipe 16 of connecting the collection room. The left side and the right side of the whole culture dish 10 are respectively provided with an air pipe 17 and a water pipe 18 which are respectively connected with an air pump 19 and a water pump 20, the air pump 19 and the water pump 20 are respectively connected with an air inlet pipe 21 and a water inlet pipe 23, wherein the water inlet pipe 23 is connected with a water tank 24, and a microorganism filtering device 22 is arranged between the air inlet pipe 21 and the air pump 19. The culture chamber is separated from the collection chamber by an insulating panel 25.

The collection chamber is provided with a chamber door 26 which can be opened and closed, so that the bacteria liquid collection cup 27 can be conveniently taken and placed. The upper part of the cup 27 has a first outlet pipe 16 extending from the culture chamber and serving as an inlet pipe for the cup 27, and a second outlet pipe 28 is provided on the left side, and the upper part of the second outlet pipe 28 is fixed to the top of the chamber. Be connected with flow rate controller 29 on the second drain pipe 28, the lower extreme of second drain pipe 28 corresponds gets liquid district 30, can use centrifuging tube or erlenmeyer flask to connect and get the fungus liquid when connecing the liquid, and the lower extreme of second drain pipe 28 can be disinfected with alcohol when connecing the liquid every time.

The right side surface of whole box 1 is equipped with switch 31, there is ultraviolet lamp switch 13 at the top, cultivate the room right side and be equipped with temperature regulation knob 32, humidity control knob 33, agitator switch 37 and air inlet switch 38, through opening air inlet switch 38 and humidity control knob 33, sterile outside gas and water molecule can get into the room of cultivateing through trachea 17 and water pipe 18 after the filtration, satisfy the required aerobic and humidity condition of microorganism growth, be equipped with first play liquid control switch 34 between cultivate the room and the collection room, be equipped with second play liquid control switch 35 between collection room and the district of getting liquid, it is equipped with flow rate control screen 36 (select the volume of getting liquid, the automatic display velocity of flow, press the enter work of confirm key) to get the liquid district 30 right side.

The working steps are as follows: the power switch 31 on the right side of the box body 1 is turned on, the ultraviolet lamp 14 on the top of the box body 1 is turned on, the whole culture room is sterilized for 30min, then the culture medium and the strain seed liquid are injected into the culture dish 10 through the first liquid inlet pipe 12 in sequence, the temperature adjusting knob 32 and the humidity adjusting knob 33 are adjusted, proper temperature and humidity are set, heating is carried out through the heating block 7, and the stirrer switch 37 and the air inlet switch 38 are turned on. After the bacteria liquid is cultured for a period of time, the first liquid outlet control switch 34 is opened to allow the bacteria liquid to flow into the collection cup 27, then the flow rate control screen 36 is provided with a volume to be collected, the second liquid outlet control switch 35 is opened, the liquid is automatically suspended after once liquid taking, and the same amount of bacteria liquid can be taken again after the start key on the flow rate control screen 36 is pressed.

It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all the embodiments of the present invention are not exhaustive, and all the obvious variations or modifications which are introduced in the technical scheme of the present invention are within the scope of the present invention.

Claims (10)

1. The microbial culture and quantitative liquid taking device is characterized by comprising a box body, wherein the box body sequentially comprises a culture chamber, a collection chamber and a liquid taking area from top to bottom;

the front side of the culture chamber is provided with a box door capable of being opened and closed; a transparent culture dish with a base is arranged in the culture chamber, a stirrer is fixed at the bottom of the culture dish, a first liquid inlet pipe and a first liquid outlet pipe are arranged above the culture dish, one end of the first liquid inlet pipe extends out of the top of the box body, and one end of the first liquid outlet pipe is introduced into the collection chamber; an air pipe and a water pipe are respectively arranged on two sides of the culture dish; a thermometer, a hygrometer and an ultraviolet lamp are also arranged in the culture chamber; the culture chamber and the collection chamber are separated by a heat insulation plate;

collect the room and openly be equipped with the room door that can open and shut, be equipped with the collection cup in the collection room, the top one side of collecting the cup is equipped with the first drain pipe that extends out from cultivateing the room, and the second drain pipe is installed to the opposite side, is equipped with flow rate controller on the second drain pipe, and the liquid district is got in the correspondence of second drain pipe lower extreme.

2. The microbial cultivation and quantitative liquid taking device as claimed in claim 1, wherein a temperature and humidity display screen is arranged at the upper right corner of the box door, and an observation screen is embedded in the center of the box door.

3. The apparatus according to claim 1, wherein the thermometer and hygrometer are fixed to the rear wall of the culture chamber, and the ultraviolet lamp is mounted on the top of the culture chamber.

4. The microbial cultivation and quantitative liquid taking device according to claim 1 or 3, wherein a power switch is arranged on the right side of the box body, and an ultraviolet lamp switch is arranged on the top of the box body.

5. The apparatus according to claim 1, wherein a cup holder connected to a motor is installed in the cultivation room, and the culture dish is placed on the cup holder.

6. The apparatus according to claim 1, wherein the first liquid inlet pipe is provided with a valve on a pipe located outside the tank.

7. The microbial cultivation and quantitative liquid taking device according to claim 1, wherein the air pipe is connected with an air pump, the air pump is further connected with an air inlet pipe, and a microbial filtering device is arranged between the air inlet pipe and the air pump; the water pipe is connected with a water pump, and the water pump is connected with a water tank through a water inlet pipe.

8. The microbial cultivation and quantitative liquid taking device according to claim 7, wherein a temperature adjusting knob, a humidity adjusting knob, a stirrer switch and an air inlet switch are arranged on the right side of the front surface of the cultivation chamber.

9. The microbial cultivation and quantitative liquid taking device according to claim 1, wherein a first liquid outlet control switch is arranged between the cultivation chamber and the collection chamber, a second liquid outlet control switch is arranged between the collection chamber and the liquid taking area, and a flow rate control screen is arranged on the right side of the liquid taking area.

10. The apparatus according to claim 1, wherein the liquid extraction region is a rectangular opening.

Images