CN215050131U

CN215050131U - Microbial cultivation base filling is equipped

Info

Publication number: CN215050131U
Application number: CN202121102710.7U
Authority: CN
Inventors: 黄艾祥; 柴云美; 马青雯
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2021-05-21
Filing date: 2021-05-21
Publication date: 2021-12-07
Anticipated expiration: 2031-05-21

Abstract

The utility model discloses a microbial culture medium filling equipment, which comprises a frame, wherein a partition plate is arranged in the frame, a first limiting rod is arranged in the frame, a storage tank is arranged at the upper end of the first limiting rod, a motor is arranged at the upper end of the storage tank, a stirring mechanism is arranged at the lower end of the motor, a fixed rod is arranged in the storage tank, a pipeline is arranged at the side end of the storage tank, a first water pump is arranged at the side end of the pipeline, a sterilization chamber is arranged in a filling device, a container is arranged in the sterilization chamber, a culture medium to be filled is placed in the container, and the culture medium is sterilized at high pressure; and through installing the water tank in the device, the water is carried to ration room and filling head through the water pipe and is cleaned, makes the washing of ration room and filling head more convenient, has improved the practicality of device, and the first internally mounted heating pipe of filling carries out high temperature disinfection in to the filling head, prevents that the culture medium from appearing polluting at the filling process.

Description

Microbial cultivation base filling is equipped

Technical Field

The utility model relates to a microbial cultivation field especially relates to a microbial cultivation base filling equipment.

Background

The isolated culture of the microorganism refers to separating the microorganism from a sample by a certain technical method, and repeatedly culturing and breeding the microorganism under the controllable condition of a laboratory to obtain the offspring with the same initial character. The microbial culture refers to the rapid growth and propagation of some (species) of microorganisms by means of artificially prepared culture media and artificially created culture conditions, such as culture temperature, nutrient substances, pH value and the like.

The Culture medium is required to be used for culturing the microorganisms, is artificially prepared, has nutrient components meeting the growth of the microorganisms, is a matrix suitable for the growth of the microorganisms, and can be liquid or solid. The common nutrient components for preparing the culture medium comprise: peptone, beef extract, yeast powder, etc., agar being the most commonly used coagulant for preparing solid culture medium. The German famous bacteriologist Koch (R.Koch, 1843-1910) successfully prepared the solid medium and invented the culture dish, forming a plate (dish) separation culture technique. The plate culture technique is widely used for separating and culturing various microorganisms from various environmental samples and human body samples (pathological tissues, secretions and the like), and researching the characteristics, functions and the like of the separated and cultured microorganisms, thereby greatly promoting the development of bacteriology and microorganism culture techniques. Culture mediums are increasing in variety, and besides beef extract peptone culture mediums and nutrient agar culture mediums, substances such as sugar, blood, serum and the like are added into the culture mediums for separating certain special groups of bacteria, so that a basal culture medium, a sugar fermentation culture medium, a blood culture medium, an enrichment culture medium, a separation culture medium and the like are formed. The increase of the variety of the culture medium reflects the deepening of understanding of the physiological characteristics of the microorganisms; meanwhile, the accumulation and enrichment of the physiological knowledge of the microorganisms promote the design and development of new microorganism isolation culture techniques and methods. It can be said that there is no culture medium invention and development of culture technology, and there is no modern microbiology, and the increase of culture medium variety can be used for preparing specific culture medium according to different varieties and life habits of microorganism, so as to attain the goal of separating and purifying culture microorganism.

The culture period of the microbial strains is generally 24-48 h, and after 24-48 h, the strains need to be streaked again to a new plate culture medium for continuous separation culture. About several hundred dishes per day require the addition of fresh medium for isolated culture of microorganisms. The general operation is as follows: after the culture medium is prepared, the culture medium is sterilized at high temperature for split charging, but the efficiency is lower, and the filling efficiency of the culture medium is improved by adopting culture medium filling equipment at present.

The existing culture medium filling equipment is generally provided with no disinfection and sterilization part, so that the interior of the culture medium is easy to generate bacterial pollution when filling is carried out, the culture medium containing bacteria cannot be used for culturing microorganisms, and the quality and the practicability of the culture medium are reduced; and the inside self-cleaning device that does not set up of current filling equipment, the filling head need wash when carrying out the filling of different materials, but the washing in ration room and the filling head is inconvenient, has reduced the practicality of device.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a microbial cultivation base filling equipment to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a microbial cultivation medium filling device comprises a rack, wherein a partition plate is arranged inside the rack, a first limiting rod is arranged inside the rack, a storage tank is arranged at the upper end of the first limiting rod, a motor is arranged at the upper end of the storage tank, a stirring mechanism is arranged at the lower end of the motor, a fixing rod is arranged inside the storage tank, a pipeline is arranged at the side end of the storage tank, a first water pump is arranged at the side end of the pipeline, a sterilizer is arranged at the side end of the rack, a sterilization chamber is arranged inside the sterilizer, a heating plate is arranged at the lower end of the sterilization chamber, a container is arranged inside the sterilization chamber, an inlet pipe is arranged at the upper end of the container, an exhaust valve is arranged at the upper end of the sterilizer, a thermometer is arranged at the side end of the sterilizer, an inlet hole is arranged at the side end of the sterilizer, a second limiting rod is arranged inside the rack, and a discharge pipe is arranged at the lower end of the storage tank, the quantitative chamber is installed to the discharging pipe lower extreme, the water pipe is installed to quantitative chamber upper end, the valve is installed to the water pipe side, the second water pump is installed to the water pipe side, the water tank is installed to second gag lever post upper end, the filling head is installed to quantitative chamber lower extreme, filling head internally mounted has the heating pipe, the inductor is installed to the first side of filling, the base is installed to the frame lower extreme, the culture dish is installed to the base upper end.

As a further aspect of the present invention: the container is fixedly connected with the sterilizer, the water inlet hole is fixedly connected with the sterilizer, and the heating plate is fixedly connected with the sterilizer.

As a further aspect of the present invention: the container is communicated with the interior of the storage tank through a pipeline, and the first water pump is fixedly connected with the pipeline.

As a further aspect of the present invention: the motor is fixedly connected with the storage tank, and the stirring mechanism is rotatably connected with the motor.

As a further aspect of the present invention: the water tank is fixedly connected with the quantitative chamber through a water pipe, and the second water pump and the valve are fixedly connected with the water pipe.

As a further aspect of the present invention: the filling head is fixedly connected with the quantitative chamber, and the heating pipe is fixedly connected with the filling head.

As a further aspect of the present invention: the inductor is fixedly connected with the filling head, and the culture dish is movably connected with the base.

Compared with the prior art, the beneficial effects of the utility model are that: in the utility model, the sterilization chamber is arranged in the filling device, the container is arranged in the sterilization chamber, the culture medium to be filled is put into the container, the culture medium is sterilized under high pressure, and the filling can be directly carried out after the high-pressure sterilization, so that the filling efficiency is improved; and through the cooperation of water tank, water pump, water pipe, carry ration room and filling head with water and clean, make the washing of ration room and filling head more convenient, improved the practicality of device, and the first internally mounted heating pipe of filling carries out high temperature disinfection in to the filling head, and later stage culture medium pollutes in the filling process.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a side view of the present invention;

FIG. 3 is an enlarged view of the point A in FIG. 1;

fig. 4 is an enlarged view of the position B in fig. 1 of the present invention.

The labels in the figure are: 1. a frame; 2. a partition plate; 3. a first limit rod; 4. storing the tank; 5. a motor; 6. a stirring mechanism; 7. fixing the rod; 8. a sterilizer; 9. a sterilization chamber; 10. a water inlet hole; 11. heating plates; 12. a container; 13. a pipeline; 14. a thermometer; 15. an exhaust valve; 16. a feed pipe; 17. a first water pump; 19. a discharge pipe; 20. a dosing chamber; 21. a second limiting rod; 22. a culture dish; 23. a base; 24. a water tank; 25. a water pipe; 26. a second water pump; 27. a valve; 28. a filling head; 29. heating a tube; 30. an inductor.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

Referring to fig. 1-4, in the embodiment of the present invention, a microbial culture medium filling device comprises a frame 1, a partition plate 2 is installed inside the frame 1, a first limiting rod 3 is installed inside the frame 1, a storage tank 4 is installed at the upper end of the first limiting rod 3, a motor 5 is installed at the upper end of the storage tank 4, a stirring mechanism 6 is installed at the lower end of the motor 5, a fixing rod 7 is installed inside the storage tank 4, a pipeline 13 is installed at the side end of the storage tank 4, a first water pump 17 is installed at the side end of the pipeline 13, a sterilizer 8 is installed at the side end of the frame 1, a sterilization chamber 9 is arranged inside the sterilizer 8, a heating plate 11 is installed at the lower end of the sterilization chamber 9, a container 12 is installed inside the sterilization chamber 9, a feeding pipe 16 is installed at the upper end of the container 12, an exhaust valve 15 is installed at the upper end of the sterilizer 8, a thermometer 14 is installed at the side end of the sterilizer 8, a water inlet 10 is installed at the side end of the sterilizer, a second limiting rod 21 is installed inside the frame 1, storage jar 4 lower extreme is installed and is discharged pipe 19, ration room 20 is installed to 19 lower extremes of discharging pipe, water pipe 25 is installed to ration room 20 upper ends, valve 27 is installed to the 25 side ends of water pipe, second water pump 26 is installed to the 25 side ends of water pipe, water tank 24 is installed to second gag lever post 21 upper end, filling head 28 is installed to ration room 20 lower extreme, filling head 28 internally mounted has heating pipe 29, inductor 30 is installed to filling head 28 side, base 23 is installed to frame 1 lower extreme, culture dish 22 is installed to base 23 upper end.

Wherein,

container

12 and 8 fixed connection of sterilizer, and

inlet opening

10 and 8 fixed connection of sterilizer, and

hot plate

11 and 8 fixed connection of sterilizer, during the use, put into container 12 with the culture medium, give the inside water that adds of sterilizer through inlet opening 10, open hot plate 11 and heat 9 inside heats of sterilization room to the culture medium to in the container 12 heats the sterilization.

The container 12 is communicated with the interior of the storage tank 4 through the pipeline 13, the first water pump 17 is fixedly connected with the pipeline 13, and during use, the first water pump 17 is started, so that the sterilized culture medium enters the storage tank 4 through the pipeline 13 to be stored, and the culture medium is convenient to convey.

Motor 5 and storage jar 4 fixed connection, and rabbling mechanism 6 rotates with motor 5 to be connected, during the use, starter motor 5 makes rabbling mechanism 6 rotatable stir the culture medium in the storage jar 4 for the cooling, the culture medium temperature after the sterilization is higher, need can the filling to 60-70 ℃.

The water tank 24 passes through water pipe 25 and ration room 20 fixed connection, and second water pump 26 and valve 27 and water pipe 25 fixed connection, during the use, starts second water pump 26, can carry the water in the water tank 24 inside the ration room 20, washs ration room 20, makes the cleanness of ration room 20 and filling head 28 more convenient.

Filling head 28 and ration room 20 fixed connection, and heating pipe 29 and filling head 28 fixed connection, during the use, start heating pipe 29 can carry out high temperature disinfection to filling head 28, is favorable to the maintenance to the culture medium.

Inductor 30 and filling head 28 fixed connection, and culture dish 22 and base 23 swing joint, during the use, survey the position of bottom culture dish 22 through inductor 30, when culture dish 22 is at the first 28 lower extremes of filling, inductor 30 can survey culture dish 22 and control filling head 28 and carry out the filling to culture dish 22, makes the device realize automatic filling.

The utility model discloses a theory of operation is: firstly, a culture medium is added into a container 12 through a feeding pipe 16, water is added into a sterilization chamber 9 through a water inlet hole 10, an exhaust valve 15 is opened, air in the sterilization chamber 9 is exhausted, a heating plate 11 is started, water in the sterilization chamber 9 is heated, the culture medium in the container 12 is sterilized at high pressure, the culture medium is not in contact with the inner wall 9 of the sterilization chamber, the temperature in the sterilization chamber 9 is monitored through a thermometer 14, the temperature and the air pressure in the sterilization chamber 9 are displayed and kept, after sterilization is completed, the exhaust valve 15 is opened, the air pressure in the sterilization chamber 9 is exhausted completely, a first water pump 17 is started, the culture medium in the container 12 is conveyed into a storage tank 4 through the first water pump 17, a motor 5 is started, the high-temperature culture medium is stirred, and filling is started after cooling to 45-50 ℃. Culture medium accessible outlet pipe 25 gets into ration room 20 in, carries out the filling through filling head 28, after the filling, can start second water pump 26, cleans ration room 20 and filling head 28, opens heating pipe 29, carries out high temperature disinfection to filling head 28 inside.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A microbiological culture medium filling equipment, includes frame (1), its characterized in that: frame (1) internally mounted has baffle (2), frame (1) internally mounted has first gag lever post (3), storage jar (4) is installed to first gag lever post (3) upper end, motor (5) are installed to storage jar (4) upper end, rabbling mechanism (6) are installed to motor (5) lower extreme, storage jar (4) internally mounted has dead lever (7), pipeline (13) are installed to storage jar (4) side, first water pump (17) are installed to pipeline (13) side, sterilizer (8) are installed to frame (1) side, sterilizer (8) inside is equipped with sterilization room (9), hot plate (11) are installed to sterilization room (9) lower extreme, sterilization room (9) internally mounted has container (12), inlet pipe (16) are installed to container (12) upper end, discharge valve (15) are installed to sterilizer (8) upper end, thermometer (14) are installed to steriliser (8) side, inlet opening (10) are installed to steriliser (8) side, frame (1) internally mounted has second gag lever post (21), discharging pipe (19) are installed to storage jar (4) lower extreme, ration room (20) are installed to discharging pipe (19) lower extreme, water pipe (25) are installed to ration room (20) upper end, valve (27) are installed to water pipe (25) side, second water pump (26) are installed to water pipe (25) side, water tank (24) are installed to second gag lever post (21) upper end, filling head (28) are installed to ration room (20) lower extreme, filling head (28) internally mounted has heating pipe (29), inductor (30) are installed to filling head (28) side, base (23) are installed to frame (1) lower extreme, a culture dish (22) is arranged at the upper end of the base (23).

2. The microbial culture medium filling equipment of claim 1, wherein: the container (12) is fixedly connected with the sterilizer (8), the water inlet hole (10) is fixedly connected with the sterilizer (8), and the heating plate (11) is fixedly connected with the sterilizer (8).

3. The microbial culture medium filling equipment of claim 1, wherein: the container (12) is communicated with the interior of the storage tank (4) through a pipeline (13), and the first water pump (17) is fixedly connected with the pipeline (13).

4. The microbial culture medium filling equipment of claim 1, wherein: the motor (5) is fixedly connected with the storage tank (4), and the stirring mechanism (6) is rotatably connected with the motor (5).

5. The microbial culture medium filling equipment of claim 1, wherein: the water tank (24) is fixedly connected with the quantitative chamber (20) through a water pipe (25), and the second water pump (26) and the valve (27) are fixedly connected with the water pipe (25).

6. The microbial culture medium filling equipment of claim 1, wherein: the filling head (28) is fixedly connected with the quantitative chamber (20), and the heating pipe (29) is fixedly connected with the filling head (28).

7. The microbial culture medium filling equipment of claim 1, wherein: inductor (30) and filling head (28) fixed connection, just culture dish (22) and base (23) swing joint.