CN213895883U

CN213895883U - A culture tank with separation function

Info

Publication number: CN213895883U
Application number: CN202022564862.0U
Authority: CN
Inventors: 闫皙; 郗艳菊; 冯金民; 谷文玲; 杨春梅; 郭永红
Original assignee: Hebei Weierli Animal Pharmacy Group Co ltd
Current assignee: Hebei Weierli Animal Pharmacy Group Co ltd
Priority date: 2020-11-09
Filing date: 2020-11-09
Publication date: 2021-08-06
Anticipated expiration: 2030-11-09

Abstract

The utility model discloses a culture tank with a separation function, which comprises a tank body, support legs are welded on the bottom of the tank body, and a sealing cover is hinged on the top of the tank body, and a feed material is welded through one end of the top of the sealing cover. The top of the sealing cover is provided with a servo motor, the output shaft of the servo motor is connected with a stirring rod, and the outer wall of one side of the top of the tank is connected with a sterile air inlet pipe, and one end of the sterile air inlet pipe is located inside the tank body. A gas distribution pipe, and a first discharge pipe is welded at the center of the bottom of the tank. By arranging a stirring rod inside the tank body, the stirring rod can be used for good stirring and mixing when a variety of different bacterial species are mixed, and a heating chamber is set outside the tank body, and when the temperature needs to be heated, a water bath is used for heating, The tank body can be heated more evenly, and when it is necessary to cool down, the electric heating wire can be turned off, and the circulating cold water can be directly introduced to cool down, which increases the practicability of the device.

Description

Culture tank with separate function

Technical Field

The utility model relates to a microbial cultivation technical field especially relates to a culture tank with separate function.

Background

Tank culture is one of the methods for mass culture of microorganisms or animal and plant cells. Adding the culture solution into a closed iron or stainless steel tank, sterilizing, inoculating microorganism or other cells to be cultured, introducing sterile air, and culturing with a stirrer. To eliminate foam, soybean oil or silicone resin is added. The culture temperature is adjusted to 20-25 ℃; actinomycete at 27-34 deg.c and plant cell at 20-34 deg.c. The tank culture is used for producing products of aerobic microorganisms such as antibiotics and citric acid, and collecting plant tissue culture cells (such as tobacco cells) in large quantity. However, most of culture tanks do not have the separation function, so when the strain needs to be sampled and detected in the culture process, the culture solution at the pipe orifice is polluted and flows back to the interior of the culture tank, thereby causing pollution and influencing the experimental result.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing a culture tank with a separation function.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a culture tank with a separation function comprises a tank body, wherein supporting legs are welded at four corners of the bottom of the tank body, a sealing cover is hinged to the top of the tank body, one end of the top of the sealing cover is welded with a feeding pipe in a penetrating mode, a servo motor is arranged at the top of the sealing cover, an output shaft of the servo motor is connected with a stirring rod, an aseptic air inlet pipe is connected to the outer wall of one side of the top of the tank body, one end, located inside the tank body, of the aseptic air inlet pipe is communicated with an annular air distribution pipe, a first material discharge pipe is welded at the central position of the bottom of the tank body, a temperature sensor is fixed to the inner wall of one side of the bottom of the tank body, a connecting pipe is welded on the outer wall of one side of the bottom of the tank body, one end, away from the tank body, of the connecting pipe is connected with a sampling chamber, an ultraviolet sterilizing lamp is installed on the inner wall of the top of the sampling chamber, a second material discharge pipe is welded at one end of the bottom of the sampling chamber, and a heating chamber is welded on the outer wall of the middle part of the tank body, the heating chamber is internally provided with electric heating wires distributed at equal intervals, the outer wall of one side of the heating chamber is communicated with a water inlet pipe, and the outer wall of the other side of the heating chamber is communicated with a water discharge pipe.

Preferably, the external portion of the tank body is provided with a PLC controller, and the PLC controller is connected with a servo motor, a temperature sensor, an ultraviolet sterilizing lamp and an electric heating wire through wires.

Preferably, the servo motor, the temperature sensor, the ultraviolet sterilizing lamp and the electric heating wire are all connected with a switch through wires, and the switch is connected with an external power supply.

Preferably, the valve is installed to the clamp in inlet pipe, aseptic intake pipe, first row of material pipe, connecting pipe, second row of material pipe, inlet tube and drain pipe middle part all.

Preferably, the top of the heating chamber is provided with exhaust holes distributed at equal intervals.

Preferably, a sealing cover is arranged outside the servo motor, and heat conduction fins distributed at equal intervals are welded on the outer wall of the sealing cover.

The utility model has the advantages that:

1. according to the culture tank with the separation function, the connecting pipe is arranged at the bottom of the tank body, a small amount of culture solution can be discharged by controlling the connecting pipe, so that sampling detection can be performed, the discharged liquid can be well separated, the polluted liquid is prevented from flowing back, the culture solution entering the sampling chamber can be cleaned after sampling is finished, and the ultraviolet sterilizing lamp is used for sterilizing, so that the culture solution in the tank body is prevented from being polluted;

2. this design has the culture tank of separating function, through set up the puddler at jar internal portion, when multiple different bacterial mixes, can utilize the puddler to carry out good stirring and mix to set up the heating chamber at jar external portion, when needs heat up, utilize the water bath heating, can let jar more even that the body is heated, and when needs cool down, can close electric heating wire, directly let in circulation cold water and cool down, increased the practicality of device.

Drawings

FIG. 1 is a sectional view showing the overall structure of a culture tank with a partition function according to the present invention;

FIG. 2 is a schematic view of the overall structure of a culture tank with a partition function according to the present invention;

FIG. 3 is an enlarged schematic view of a part of the structure of a culture tank with a partition function according to the present invention.

In the figure: 1 jar body, 2 supporting legs, 3 sealed lids, 4 inlet pipes, 5 servo motor, 6 puddlers, 7 aseptic intake pipe, 8 annular gas distribution pipes, 9 first row of material pipes, 10 temperature sensor, 11 connecting pipes, 12 sampling chambers, 13 sterilamp, 14 second row of material pipes, 15 heating chambers, 16 electric heating wire, 17 inlet tubes, 18 drain pipes.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-3, a culture tank with a separation function comprises a tank body 1, wherein supporting legs 2 are welded at four corners of the bottom of the tank body 1, a sealing cover 3 is hinged at the top of the tank body 1, a feeding pipe 4 is welded at one end of the top of the sealing cover 3 in a penetrating manner, a servo motor 5 is arranged at the top of the sealing cover 3, a sealing cover is arranged outside the servo motor 5, heat conducting fins distributed at equal intervals are welded on the outer wall of the sealing cover, an output shaft of the servo motor 5 is connected with a stirring rod 6, a sterile air inlet pipe 7 is connected with the outer wall of one side of the top of the tank body 1, one end of the sterile air inlet pipe 7, which is positioned inside the tank body 1, is communicated with an annular air distribution pipe 8, a first discharging pipe 9 is welded at the central position of the bottom of the tank body 1, a temperature sensor 10 is fixed on the inner wall of one side of the bottom of the tank body 1, a connecting pipe 11 is welded on the outer wall of the one side of the bottom of the tank body 1, and a sampling chamber 12 is connected with the connecting pipe 11, an ultraviolet germicidal lamp 13 is mounted on the inner wall of the top of the sampling chamber 12, a connecting pipe 11 is arranged at the bottom of the tank body 1, a small amount of culture solution can be discharged by controlling the connecting pipe 11, so that sampling detection can be performed, the discharged liquid can be well separated, the backflow of the polluted liquid is avoided, the culture solution entering the sampling chamber 12 can be cleaned after sampling is completed, and the ultraviolet germicidal lamp 13 is used for sterilization, so that the culture solution in the tank body 1 is prevented from being polluted;

the inner wall of one end of the bottom of the sampling chamber 12 is welded with a second discharging pipe 14, the outer wall of the middle part of the tank body 1 is welded with a heating chamber 15, the outside of the tank body 1 is provided with a PLC controller, the PLC controller is connected with a servo motor 5, a temperature sensor 10, an ultraviolet germicidal lamp 13 and an electric heating wire 16 through leads, the electric heating wire 16 distributed at equal intervals is arranged inside the heating chamber 15, the top of the heating chamber 15 is provided with exhaust holes distributed at equal intervals, the servo motor 5, the temperature sensor 10, the ultraviolet germicidal lamp 13 and the electric heating wire 16 are all connected with a switch through leads, the switch is connected with an external power supply, the outer wall of one side of the heating chamber 15 is communicated with a water inlet pipe 17, the outer wall of the other side of the heating chamber 15 is communicated with a water outlet pipe 18, the middle parts of the water inlet pipe 4, the sterile air inlet pipe 7, the first discharging pipe 9, the connecting pipe 11, the second discharging pipe 14, the water inlet pipe 17 and the water outlet pipe 18 are oppositely clamped with valves, through set up puddler 6 in jar internal portion, when multiple different bacterial mixes, can utilize puddler 6 to carry out good stirring and mix to set up heating chamber 15 in jar external portion of body 1, when needs heat up, utilize the water bath heating, can let jar body 1 be heated more even, and when needs cool down, can close electric heating wire 16, directly let in circulation cold water and cool down, increased the practicality of device.

The working principle is as follows: when the culture tank with the separation function is used, firstly, the servo motor 5, the temperature sensor 10, the ultraviolet sterilization lamp 13 and the electric heating wire 16 are connected with an external power supply through leads, then strains to be cultured are poured into the tank body 1 from the feeding pipe 4, then the servo motor 5 is started, the servo motor 5 is utilized to drive the stirring rod 6 to stir and mix, when the strains need to be heated, cold water is injected into the water inlet pipe 17, the electric heating wire 16 is started to heat the cold water, the PLC controller is utilized to timely control the electric heating die 16, the temperature sensor 10 timely knows the internal temperature of the tank body 1, sterile air is shunted to the annular gas shunt pipe 8 through the sterile air inlet pipe 7, when the culture is finished and needs to be checked, the valve of the connecting pipe 11 is opened, a little culture solution flows into the sampling chamber 12, then the valve is closed, and the liquid in the sampling chamber 12 is sampled, after the sampling is completed, the inside of the sampling chamber 12 is cleaned, and the ultraviolet germicidal lamp 13 is turned on to perform a sterilization process.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A culture tank with a separation function comprises a tank body (1) and is characterized in that supporting legs (2) are welded at four corners of the bottom of the tank body (1), a sealing cover (3) is hinged to the top of the tank body (1), an inlet pipe (4) penetrates through and is welded at one end of the top of the sealing cover (3), a servo motor (5) is arranged at the top of the sealing cover (3), an output shaft of the servo motor (5) is connected with a stirring rod (6), an aseptic air inlet pipe (7) is connected to the outer wall of one side of the top of the tank body (1), one end of the aseptic air inlet pipe (7) located inside the tank body (1) is communicated with an annular air distribution pipe (8), a first exhaust pipe (9) is welded at the central position of the bottom of the tank body (1), a temperature sensor (10) is fixed to the inner wall of one side of the bottom of the tank body (1), and a connecting pipe (11) is welded to the outer wall of one side of the bottom of the tank body (1), the one end that the jar body (1) was kept away from in connecting pipe (11) is connected with sampling chamber (12), and sampling chamber (12) top inner wall installs sterilamp (13), the welding of sampling chamber (12) bottom one end inner wall has the second to arrange material pipe (14), and the welding of the outer wall in the middle part of the jar body (1) has heating chamber (15), heating chamber (15) inside is equipped with electric heating wire (16) that the equidistance distributes, heating chamber (15) one side outer wall intercommunication has inlet tube (17), and heating chamber (15) opposite side outer wall intercommunication has drain pipe (18).

2. The cultivation tank with the partition function as claimed in claim 1, wherein a PLC controller is installed outside the tank body (1), and the PLC controller is connected with the servo motor (5), the temperature sensor (10), the ultraviolet sterilization lamp (13) and the electric heating wire (16) through wires.

3. The culture tank with the partition function according to claim 1, wherein the servo motor (5), the temperature sensor (10), the ultraviolet sterilizing lamp (13) and the electric heating wire (16) are all connected with a switch through wires, and the switch is connected with an external power supply.

4. The culture tank with the separation function as recited in claim 1, wherein the feeding pipe (4), the sterile air inlet pipe (7), the first discharging pipe (9), the connecting pipe (11), the second discharging pipe (14), the water inlet pipe (17) and the water outlet pipe (18) are provided with valves at the middle part in a butt-clamping manner.

5. The culture pot with partition function as claimed in claim 1, wherein the top of the heating chamber (15) is provided with exhaust holes distributed equidistantly.

6. The culture tank with the partition function as claimed in claim 1, wherein the servo motor (5) is externally provided with a sealing cover, and the outer wall of the sealing cover is welded with heat conduction fins distributed at equal intervals.