CN217025964U - Microbial inoculant continuous production device - Google Patents
Microbial inoculant continuous production device Download PDFInfo
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- CN217025964U CN217025964U CN202220512803.5U CN202220512803U CN217025964U CN 217025964 U CN217025964 U CN 217025964U CN 202220512803 U CN202220512803 U CN 202220512803U CN 217025964 U CN217025964 U CN 217025964U
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Abstract
The utility model discloses a microbial agent continuous production device, which comprises a fermentation tank body, wherein the fermentation tank body is fixed on a supporting component, and the outer surface of the fermentation tank body is provided with a heat-insulating layer; a bacterial liquid discharge port is formed in the bottom of the fermentation tank body, and a first electromagnetic valve is fixed at the bacterial liquid discharge port; the top of the fermentation tank body is provided with a culture medium feed inlet and a microorganism inoculation port, and a second electromagnetic valve is fixed at the culture medium feed inlet; an aeration outlet of the aeration unit is communicated with the interior of the fermentation tank body; the culture medium feeding unit is connected with a culture medium feeding hole; the photoelectric control unit comprises a photoelectric controller which is electrically connected with a temperature sensor, an oxygen concentration sensor and a bacteria liquid concentration detection probe which are positioned on the inner side of the fermentation tank body; the utility model monitors the concentration of the bacterial liquid in the fermentation tank body and the growth stage in real time through the photoelectric control unit, and controls the feeding and discharging through the electromagnetic valve so as to automatically control the discharging of the bacterial liquid in the logarithmic growth phase, thereby realizing continuous production.
Description
Technical Field
The utility model belongs to the field of microbial agent production equipment, and particularly relates to a continuous production device for a microbial agent.
Background
The microbial agent is a liquid preparation containing a certain functionalized strain prepared by carrying out amplification culture on target microorganisms by a certain process, is used for bioremediation of polluted water, soil improvement and the like, and has the characteristics of economy, high efficiency, low energy consumption, no secondary pollution and the like.
The production of common microbial agents generally adopts batch culture and continuous culture modes. The batch culture is a culture mode in which after a certain amount of culture medium is put into a closed reactor, the microorganisms are inoculated, grown and propagated, and the thalli are aged, and then the culture is finished, and finally the product is extracted. The inoculation work of each batch needs a series of classification and expansion culture processes of strains, the process flow is long, the operation is complex, and the continuity of production cannot be ensured. And the repeated inoculation is easy to introduce mixed bacteria, so that the microbial inoculum pollution is caused, and the quality of the microbial inoculum is influenced.
And the continuous production mode of the microbial agent can avoid the complex operation of multiple times of inoculation of the microorganisms, and can effectively avoid the problems.
SUMMERY OF THE UTILITY MODEL
The utility model provides a continuous production device of a microbial agent, which monitors the concentration of bacterial liquid in a fermentation tank body and the growth stage in real time through a photoelectric control unit, controls feeding and discharging through an electromagnetic valve, and automatically controls the discharge of the bacterial liquid in the logarithmic growth phase to realize continuous production.
The utility model provides a continuous production device of microbial inoculum, comprising:
the fermentation tank body is fixed on the supporting component; the outer surface of the fermentation tank body is provided with a heat insulation layer, and a stirring mechanism is fixed in the fermentation tank body; a bacteria liquid discharge port is formed in the bottom of the fermentation tank body, and a first electromagnetic valve is fixed at the bacteria liquid discharge port; the top of the fermentation tank body is provided with a culture medium feed inlet and a microorganism inoculation port, and a second electromagnetic valve is fixed at the culture medium feed inlet;
the aeration outlet of the aeration unit is communicated with the inside of the fermentation tank body;
the culture medium feeding unit is connected with the culture medium feeding hole;
an optoelectronic control unit comprising: the temperature sensor is fixed on the inner side of the fermentation tank body; the oxygen concentration sensor is fixed on the inner side of the fermentation tank body; the bacterial liquid concentration detection probe is fixed on the inner side of the fermentation tank body and is in contact with the bacterial liquid; and the photoelectric controller is connected with the first electromagnetic valve, the second electromagnetic valve, the temperature sensor, the oxygen concentration sensor and the bacteria liquid concentration detection probe.
The aeration unit comprises:
the microporous disc aerator is fixed at the bottom of the inner side of the fermentation tank body; aeration holes are formed in the microporous disc type aerator;
one end of the air pipe penetrates through a through hole formed in the fermentation tank body and is connected with an air inlet of the microporous disc type aerator;
the air outlet of the air filter is connected with the other end of the air pipe;
the air outlet of the air compressor is connected with the air inlet of the air filter; the air compressor is electrically connected with the photoelectric controller.
The aerator is provided with a plurality of aeration holes in an array.
The bacteria liquid concentration detection probe consists of a xenon lamp, a monochromator, a glass plate and an inductor.
The culture medium feeding unit comprises:
the discharge port of the high-temperature steam sterilization device is connected with the feed port of the culture medium through a pipeline;
the culture medium feeding tank is characterized in that a discharge port at the bottom of the culture medium feeding tank is connected with a feed port of the high-temperature steam sterilization device through a pipeline.
The top of the heat-insulating layer is provided with a circulating water inlet, and the bottom of the heat-insulating layer is provided with a circulating water outlet.
The stirring mechanism comprises a driving motor, the driving motor is fixed on the top surface of the fermentation tank body, the bottom end of a driving shaft of the driving motor penetrates through the top surface of the fermentation tank body, and a bottom stirring paddle is fixed at the bottom end of the driving shaft.
The supporting assembly comprises a bottom plate, a supporting frame is fixed on the upper surface of the bottom plate, and the fermentation tank body is fixed on the supporting frame.
The photoelectric controller is fixed on the bottom plate.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model monitors the concentration of the bacterial liquid in the fermentation tank body and the growth stage in real time through the photoelectric control unit, and controls the feeding and discharging through the electromagnetic valve so as to automatically control the discharge of the bacterial liquid in the logarithmic growth phase, thereby realizing continuous production.
2. According to the utility model, the bacterial liquid in logarithmic growth phase is continuously kept in the tank body, so that microorganism inoculation is not required subsequently, only feeding of a sterile culture medium and discharging of the bacterial liquid are required, the production procedure is simplified, and the pollution opportunity is effectively reduced.
3. The utility model provides a novel ultraviolet spectroscopic probe which is used as a bacteria liquid concentration detection probe, directly measures the bacterial cell concentration, is more accurate than a turbidity probe or a potential detection probe used in the prior art, and improves the quality of bacteria liquid production.
Drawings
Fig. 1 is a front view of the overall structure of the present invention.
Description of reference numerals:
1. a fermentation tank body; 2. a support frame; 3. a base plate; 4. an aeration unit; 5. a medium feeding unit; 6. A photoelectric control unit; 7. a drive motor; 8. an air compressor; 9. an air filter; 10. a microbial inoculation port; 11. a culture medium feeding tank; 12. a high-temperature steam sterilization device; 13. a first solenoid valve; 14. A culture medium feed port; 15. a second solenoid valve; 16. a drive shaft; 17. a bacteria liquid concentration detection probe; 18. A heat-insulating layer; 19. a microporous disc aerator; 20. a stirring paddle; 21. a bacterial liquid discharge port; 22. a photoelectric controller; 23. an air tube; 24. a temperature sensor; 25. an oxygen concentration sensor; 26. a circulating water inlet; 27. and a circulating water outlet.
Detailed Description
One embodiment of the present invention is described in detail below with reference to fig. 1, but it should be understood that the scope of the present invention is not limited by the embodiment.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on those shown in the drawings, merely for convenience of description and simplification of the technical solution of the present invention, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1:
as shown in fig. 1, the continuous production apparatus for microbial inoculum provided by the embodiment of the utility model comprises a fermentation tank body 1, a support frame 2, a bottom plate 3, an aeration unit 4, a culture medium feeding unit 5 and a photoelectric control unit 6; the middle part of the upper surface of the bottom plate 3 is connected with the bottom end of the fermentation tank body 1 through the support frame 2, and the right side of the upper surface of the bottom plate 3 is provided with the photoelectric control unit 6. The outer surface of the fermentation tank body 1 is provided with an insulating layer 18 to maintain the temperature condition required by the growth of microorganisms. The top surface of the fermentation tank body 1 is welded with a driving motor 7, a driving shaft 16 of the driving motor 7 penetrates through the top surface of the fermentation tank body, a bottom stirring paddle 20 is welded at the bottom end of the driving shaft 16 of the driving motor 7, the driving motor 7 drives the stirring paddle 20 to rotate at a certain speed, and the microbial inoculum and the culture medium in the tank body 1 are fully stirred, so that the quality of microbial inoculum products is uniform and stable; the bottom surface welding of the fermentation tank body 1 has fungus liquid discharge gate 21, and discharge gate 21 department is fixed with first solenoid valve 13, and the switch of discharge gate 21 is controlled by first solenoid valve 13, and 13 electric connections photoelectric controller 5 of first solenoid valve pass through steps such as pipeline entering production goes up subsequent microbial inoculum drying after the microorganism ejection of compact. The top of the fermentation tank body 1 is provided with a culture medium feeding hole 14 and a microorganism inoculation port 10, the culture medium feeding hole 14 is controlled by a second electromagnetic valve 15, a discharging hole 21 is controlled by a first electromagnetic valve 13, and the first electromagnetic valve 13 and the second electromagnetic valve 15 are connected with a photoelectric controller 22 through a control circuit.
The top of the heat-insulating layer 18 arranged on the outer surface of the fermentation tank body 1 is provided with a circulating water inlet 26, and the bottom of the heat-insulating layer 18 is provided with a circulating water outlet 27. The water inlet temperature is continuously adjusted according to the fermentation conditions of the strains and the actual temperature in the tank body.
The aeration unit 4 consists of an air compressor 8, an air filter 9 and a microporous disc aerator 19 arranged at the bottom of the fermentation tank body 1, and a plurality of aeration holes are arrayed on the microporous disc aerator 19. An air outlet of the air compressor 8 is connected with an air inlet of an air filter 9, an air outlet of the air filter 9 is connected with one end of an air pipe 23, and the other end of the air pipe 23 penetrates through a through hole in the left side of the tank body 1 to be connected with an air inlet of an aerator 19; the input end of the air compressor 8 is electrically connected with the output end of the photoelectric controller 21, the aeration quantity can be controlled according to the dissolved oxygen quantity of bacteria liquid, and the air sterilized by the filter 9 is uniformly conveyed into the tank body 1, so that the fermentation efficiency of the strains is improved, and the effect of the microbial inoculum is improved.
The culture medium feeding unit 5 consists of a culture medium feeding tank 11, a high-temperature steam sterilization device 12 and a connecting pipeline. The bottom discharge port of the culture medium feeding tank 11 is connected with the feed port of the high-temperature steam sterilization device 12 through a pipeline, the discharge port of the high-temperature sterilization device 12 is connected with the culture medium feed port 14 at the top of the fermentation tank body 1 through a pipeline, and a second electromagnetic valve 15 is arranged above the feed port 14. Technicians configure different culture media according to different microbial agents, place the configured culture media into a culture medium feeding tank 11, sterilize the culture media through a high-temperature steam sterilization device 12, and automatically flow a sterile culture medium into the fermentation tank body 1 to supplement nutrients for the bacteria liquid when a second electromagnetic valve 15 is opened.
The photoelectric control unit 6 is composed of a photoelectric controller 22, a bacteria liquid concentration detection probe 17, a temperature sensor 24, an oxygen concentration sensor 25, a first electromagnetic valve 13, a second electromagnetic valve 15 and a connecting circuit. The temperature sensor 24 and the oxygen concentration sensor 25 are arranged on the right side in the tank body 1, and the temperature sensor 24 and the oxygen concentration sensor 25 are electrically connected with the photoelectric controller 22, so that the temperature and the oxygen concentration of the bacteria liquid can be monitored at any time, and an operator can adjust the bacteria liquid timely according to data. The inner side of the fermentation tank body 1 is also provided with a bacteria liquid concentration detection probe 17 which is contacted with the bacteria liquid, the bacteria liquid concentration detection probe 17 is connected with the tail end of an electric wire, and the electric wire passes through a through hole arranged on the fermentation tank body 1 and is connected with a photoelectric controller 22 fixed on the bottom plate 3.
The working principle of the utility model is as follows:
when in use: and (3) switching on an external power supply, enabling all electrical appliances to work normally, firstly putting the culture medium treated by the culture medium feeding unit 5 into the fermentation tank body 1, and then inoculating the microorganism seed liquid through the microorganism inoculation port 10. The driving motor 7 is started, and the motor 7 drives the stirring paddle 20 welded at the bottom end of the driving shaft 16 to rotate, so that the bacteria liquid and the culture medium in the tank body 1 are fully stirred.
The right side of the interior of the tank body 1 is also respectively provided with a temperature sensor 24 and an oxygen concentration sensor 25, so that the temperature and the oxygen concentration in the tank body 1 can be monitored at any time, and an operator can adjust the temperature and the oxygen concentration timely according to data. When the temperature of the bacteria liquid needs to be adjusted, the water inlet temperature of the heat-insulating layer 18 is changed until the temperature displayed by the photoelectric controller 22 meets the requirement. When the oxygen concentration of the bacteria liquid needs to be adjusted, the air compressor 8 is started, compressed air is sterilized through the air filter 9 and then is conveyed to the microporous disc type aerator 19 through the air pipe 23, the compressed air is sprayed to the inside of the tank body 1 through the aeration holes arrayed in the aerator 19, the bacteria liquid in the tank body 1 is uniformly aerated, oxygen required by the growth of microorganisms is provided, meanwhile, the aeration process is also beneficial to uniformly mixing the bacteria liquid, the fermentation efficiency of the strains is improved, and the effect of the microbial inoculum is improved.
In the fermentation process of the bacterial liquid, the growth state of the strains is detected in real time by the bacterial liquid concentration detection probe 17, when the detected 0D600 reaches a certain range required, the photoelectric controller 22 controls the first electromagnetic valve 13 and the second electromagnetic valve 15 to be opened, the bacterial liquid finished product reaching the requirement is discharged from the bacterial liquid discharge port 21, and meanwhile, the culture medium feed port 14 is used for supplementing the culture medium. The photoelectric controller 22 controls the opening and closing of the first electromagnetic valve 13 and the second electromagnetic valve 15 through the 0D600 value detected by the monitoring probe 17, so that the concentration of the bacterial liquid in the tank body 1 is kept stable, and the continuous production of the bacterial liquid is realized.
In summary, the present invention provides a continuous production apparatus for microbial agents, which monitors the concentration of bacterial liquid in the fermentation tank and the growth stage in real time by a photoelectric control unit, and controls the feeding and discharging of the bacterial liquid by an electromagnetic valve, so as to automatically control the discharge of the bacterial liquid during the logarithmic growth phase, thereby realizing continuous production.
The above disclosure is only for a few specific embodiments of the present invention, however, the present invention is not limited to the above embodiments, and any variations that can be made by those skilled in the art are intended to fall within the scope of the present invention.
Claims (9)
1. A microbial inoculant continuous production device, characterized by comprising:
the fermentation tank body (1) is fixed on the supporting component; the outer surface of the fermentation tank body (1) is provided with a heat insulation layer (18), and a stirring mechanism is fixed in the fermentation tank body (1); a bacterium liquid discharge port (21) is formed in the bottom of the fermentation tank body (1), and a first electromagnetic valve (13) is fixed at the bacterium liquid discharge port (21); the top of the fermentation tank body (1) is provided with a culture medium feed port (14) and a microorganism inoculation port (10), and a second electromagnetic valve (15) is fixed at the culture medium feed port (14);
the aeration outlet of the aeration unit (4) is communicated with the inside of the fermentation tank body (1);
the culture medium feeding unit (5) is connected with the culture medium feeding hole (14);
-a photoelectric control unit (6) comprising: the temperature sensor (24) is fixed on the inner side of the fermentation tank body (1); an oxygen concentration sensor (25) fixed inside the fermenter body (1); the bacterial liquid concentration detection probe (17) is fixed on the inner side of the fermentation tank body (1) and is in contact with the bacterial liquid; and the photoelectric controller (22) is connected with the first electromagnetic valve (13), the second electromagnetic valve (15), the temperature sensor (24), the oxygen concentration sensor (25) and the bacteria liquid concentration detection probe (17).
2. A microbial inoculant continuous production apparatus as claimed in claim 1, wherein said aeration unit (4) comprises:
the microporous disc aerator (19) is fixed at the bottom of the inner side of the fermentation tank body (1); aeration holes are formed in the microporous disc type aerator (19);
one end of the air pipe (23) penetrates through a through hole formed in the fermentation tank body (1) and is connected with an air inlet of the microporous disc type aerator (19);
the air outlet of the air filter (9) is connected with the other end of the air pipe (23);
the air outlet of the air compressor (8) is connected with the air inlet of the air filter (9); the air compressor (8) is electrically connected with the photoelectric controller (22).
3. The continuous production apparatus of microbial agents as claimed in claim 2, wherein the microporous disk aerator (19) is provided with a plurality of aeration holes in an array.
4. The continuous production device of microbial inoculum according to claim 1, characterized in that the bacteria liquid concentration detection probe (17) comprises a xenon lamp, a monochromator, a glass plate and a sensor.
5. The apparatus for continuously producing a microbial inoculum according to claim 1, wherein the medium feeding unit (5) comprises:
the discharge hole of the high-temperature steam sterilization device (12) is connected with the culture medium feed hole (14) through a pipeline;
a feed inlet of the high-temperature steam sterilization device (12) is connected with a feed outlet at the bottom of the culture medium feeding tank (11) through a pipeline.
6. The continuous production device of microbial inoculum according to claim 1, characterized in that the top of the heat-insulating layer (18) is provided with a circulating water inlet (26), and the bottom of the heat-insulating layer (18) is provided with a circulating water outlet (27).
7. The continuous production device of microbial inoculum according to claim 1, characterized in that the stirring mechanism comprises a driving motor (7), the driving motor (7) is fixed on the top surface of the fermentation tank body (1), the bottom end of a driving shaft (16) of the driving motor (7) penetrates through the top surface of the fermentation tank body (1), and the bottom end of the driving shaft (16) is fixed with a bottom stirring paddle (20).
8. The continuous production device for microbial agents as claimed in claim 1, wherein the support assembly comprises a bottom plate (3), a support frame (2) is fixed on the upper surface of the bottom plate (3), and the fermentation tank body (1) is fixed on the support frame (2).
9. A continuous production apparatus of microbial agents as claimed in claim 8, wherein said photoelectric controller (22) is fixed on said bottom plate (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220512803.5U CN217025964U (en) | 2022-03-09 | 2022-03-09 | Microbial inoculant continuous production device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220512803.5U CN217025964U (en) | 2022-03-09 | 2022-03-09 | Microbial inoculant continuous production device |
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| Publication Number | Publication Date |
|---|---|
| CN217025964U true CN217025964U (en) | 2022-07-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220512803.5U Active CN217025964U (en) | 2022-03-09 | 2022-03-09 | Microbial inoculant continuous production device |
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| CN (1) | CN217025964U (en) |
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2022
- 2022-03-09 CN CN202220512803.5U patent/CN217025964U/en active Active
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