CN113621478A - Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture - Google Patents
Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture Download PDFInfo
- Publication number
- CN113621478A CN113621478A CN202110881139.1A CN202110881139A CN113621478A CN 113621478 A CN113621478 A CN 113621478A CN 202110881139 A CN202110881139 A CN 202110881139A CN 113621478 A CN113621478 A CN 113621478A
- Authority
- CN
- China
- Prior art keywords
- plate
- guide
- nutrient solution
- frame
- guide plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 69
- 244000005700 microbiome Species 0.000 title claims abstract description 28
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 65
- 230000006835 compression Effects 0.000 claims description 41
- 238000007906 compression Methods 0.000 claims description 41
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 25
- 239000001301 oxygen Substances 0.000 claims description 25
- 229910052760 oxygen Inorganic materials 0.000 claims description 25
- 238000002347 injection Methods 0.000 claims description 24
- 239000007924 injection Substances 0.000 claims description 24
- 238000007789 sealing Methods 0.000 claims description 14
- 238000000605 extraction Methods 0.000 claims description 12
- 238000000926 separation method Methods 0.000 claims description 6
- 235000012149 noodles Nutrition 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 description 11
- 230000008569 process Effects 0.000 description 11
- 230000009471 action Effects 0.000 description 10
- 239000007789 gas Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the field of microorganism culture, in particular to a timed and equivalent nutrient solution adding device suitable for microorganism breeding culture, which comprises a bottom plate, a placing assembly, a culture dish assembly, a nutrient solution extracting assembly and the like; be equipped with on the bottom plate and place the subassembly, be equipped with the culture dish subassembly on placing the subassembly, be equipped with the nutrient solution on the bottom plate and extract the subassembly. Through the cooperation of equipment internals, every hour can start this equipment and make it add quantitative nutrient solution for next culture dish body, can make this internal nutrient solution that all adds of four culture dishes after four hours to make the growth and reproduction condition of this internal microorganism of four culture dishes unanimous, the follow-up microorganism to this internal culture dish of personnel of being convenient for is studied, has reached the effect that can regularly add equivalent nutrient solution for four culture dish bodies in proper order.
Description
Technical Field
The invention relates to the field of microbial culture, in particular to a device for adding nutrient solution in a timed and equivalent manner, which is suitable for microbial breeding culture.
Background
The technology of making some microorganisms grow and reproduce quickly by means of artificially configured culture medium and artificially created culture conditions is called microorganism culture technology, the growth and reproduction of the microorganisms need proper nutrient conditions, proper oxygen content, proper environmental temperature and the like, the culture solution is a nutrient for the growth of the microorganisms, plants and animal tissues, the nutrient solution is a novel nutrient solution synthesized by adopting an environmental bionics technology and a mycorrhizal symbiosis principle and after treatment, and the nutrient solution is the key of soilless culture.
In the process of culturing microorganisms, culture solution is generally loaded in a plurality of culture dishes in groups, and the microorganisms are cultured in the culture dishes, and in the process of culturing, in order to enable the growth and the propagation of the microorganisms to be the same, the separation of the amount of the injected nutrient solution and the time is very important, and the prior art is that the nutrient solution is manually added into the culture dishes by people, but the prior art is difficult to control the time and the amount of the added nutrient solution, and the prior art is inconvenient to inject oxygen into the culture dishes.
Disclosure of Invention
In view of the above, it is necessary to provide a device for feeding equal amounts of nutrient solution at regular time for sequential feeding of equal amounts of nutrient solution to four culture dish bodies and for automatically providing an appropriate amount of oxygen to microorganisms in the culture dish for breeding culture of microorganisms, so as to solve the problems of the prior art that it is difficult to control the feeding time and amount of nutrient solution and it is inconvenient to inject oxygen into the culture dish.
The technical scheme is as follows: the utility model provides a nutrient solution adds equipment regularly equivalent suitable for microorganism breeds cultivation, including the bottom plate, place subassembly, culture dish subassembly, nutrient solution extraction subassembly, drive assembly and supporting component, is equipped with on the bottom plate and places the subassembly, places to be equipped with the culture dish subassembly on the subassembly, is equipped with the nutrient solution extraction subassembly on the bottom plate, is equipped with drive assembly on the bottom plate, is equipped with supporting component on the drive assembly.
Preferably, the placing assembly comprises a rotating shaft and a placing disc, the rotating shaft is connected to the bottom plate in a rotating mode, and the top end of the rotating shaft is connected with the placing disc.
As preferred, the culture dish subassembly is including culture dish body, first baffle, inclined plane closing plate and fixed spring, places a set top distributing type and has placed four culture dish bodies, and the hookup has first baffle on the culture dish body, and the symmetry sliding type is connected with the inclined plane closing plate on the first baffle, and two inclined plane closing plates contact, are connected with two fixed springs between inclined plane closing plate and the first baffle.
Preferably, the nutrient solution extraction component comprises a support frame, an electric push rod, a guide frame, a movable strip, a reset spring, a rubber sleeve, a support frame, a first guide plate, a second guide plate, a first push block, a second guide plate and a guide spring, wherein the top of the bottom plate is connected with the support frame, the electric push rod is installed on the support frame, one end of the electric push rod is connected with the guide frame, the movable strip is connected with the guide frame in a sliding way, the reset spring is connected between the movable strip and the guide frame, the rubber sleeve is connected on the movable strip, the support frame is connected with the support frame, the first guide plate is connected with the support frame in a sliding way, the second guide plate is connected with the support frame, the first push block is connected with the second guide plate, the second guide plate is connected with the second push block in a sliding way, and a guide spring is connected between the second guide plate and the second guide plate.
Preferably, the driving assembly comprises a bevel rack, a guide slat, a guide rod, a first compression spring, a bevel rod and a second compression spring, the drawing board, the fluting baffle, the drive rack, third compression spring, oblique noodless and drive gear, the bottom plate top hookup has the inclined plane frame, the direction slat has been placed at the bottom plate top, the hookup has the guide bar on the direction slat, guide bar and inclined plane frame sliding type are connected, be connected with first compression spring between inclined plane frame and the direction slat, the sliding type is connected with the inclined plane pole on the direction slat, be connected with second compression spring between inclined plane pole and the direction slat, inclined plane pole one end hookup has the drawing board, the hookup has the fluting baffle on the direction slat, the sliding type is connected with the drive rack on the fluting baffle, be connected with third compression spring between drive rack and the fluting baffle, the bottom plate top hookup has oblique noodless, the hookup of rotation axis lower part has drive gear.
Preferably, the supporting assembly comprises a supporting plate, a supporting bar, a supporting spring and a separating rod, the supporting plate is connected to one side of the slotted guide plate, the supporting bar is connected to the supporting plate in a sliding mode, the supporting spring is connected between the supporting bar and the supporting plate, the separating rod is connected to the supporting frame, and the separating rod is in contact with the supporting bar.
Preferably, the oxygen injection assembly is arranged on the support frame and comprises a lower pressing plate, a homing spring, a movable plate, a fourth compression spring and an air injection pipe, the lower pressing plate is connected to the support frame in a sliding mode, the lower pressing plate is in contact with the inclined plane strip, the homing spring is connected between the support frame and the lower pressing plate, the movable plate is connected to the support frame in a sliding mode, the movable plate is in contact with the lower pressing plate, the fourth compression spring is connected between the movable plate and the support frame, and the air injection pipe is connected to the movable plate.
The invention has the beneficial effects that:
through the cooperation of equipment internals, pour into inside one of them culture dish body after the rubber head burette on the rubber sleeve sucks quantitative nutrient solution, place the dish afterwards and the device can rotate the quarter round, and the rubber head burette on the rubber sleeve can not rotate and cause the influence to placing the dish and the device on it, be convenient for add quantitative nutrient solution for next culture dish body and make preparation, reached and to add the effect to this internal culture dish with quantitative nutrient solution automatically.
Through the cooperation of equipment internals, every hour can start this equipment and make it add quantitative nutrient solution for next culture dish body, can make this internal nutrient solution that all adds of four culture dishes after four hours to make the growth and reproduction condition of this internal microorganism of four culture dishes unanimous, the follow-up microorganism to this internal culture dish of personnel of being convenient for is studied, has reached the effect that can regularly add equivalent nutrient solution for four culture dish bodies in proper order.
Through the gas injection pipe, the gas injection pipe can be injected oxygen into one of them culture dish body of having added the nutrient solution inside, has provided suitable condition for the inside microorganism of culture dish body breeds, has reached the effect that can provide suitable oxygen for this internal microorganism of culture dish automatically.
After a certain amount of nutrient solution is added to one of the culture dish bodies, the culture solution in the culture dish body can be taken out due to the rubber head dropper on the rubber sleeve, the rubber head dropper on the rubber sleeve can be manually taken out, and when the nutrient solution is required to be added to the next culture dish body, a new rubber head dropper can be sleeved on the rubber sleeve, so that the residual nutrient solution in the nutrient solution placing groove cannot be polluted by the culture solution taken out by the rubber head dropper, and the subsequent operation is facilitated.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention.
Fig. 2 is a schematic perspective view of a second embodiment of the present invention.
Fig. 3 is a partial perspective view of the present invention.
Fig. 4 is a schematic perspective view of the placement module of the present invention.
FIG. 5 is a schematic view of the assembly of the present invention in a disassembled three-dimensional configuration.
Fig. 6 is a schematic view of a first partial body structure of a nutrient solution extraction assembly of the present invention.
Fig. 7 is a schematic view of a second partial body structure of the nutrient solution extraction assembly of the present invention.
Fig. 8 is an enlarged perspective view of the present invention a.
Fig. 9 is a schematic view of a first partial body structure of the driving assembly of the present invention.
Fig. 10 is a schematic view of a second partial body structure of the driving assembly of the present invention.
Fig. 11 is a schematic perspective view of the oxygen injection assembly of the present invention.
Description of reference numerals: 1_ bottom plate, 2_ placing assembly, 21_ rotating shaft, 22_ placing tray, 3_ petri dish assembly, 31_ petri dish body, 32_ first guide plate, 33_ bevel sealing plate, 34_ stationary spring, 4_ nutrient solution extraction assembly, 41_ support frame, 42_ electric putter, 43_ guide frame, 44_ movable bar, 45_ return spring, 46_ rubber sleeve, 47_ cradling frame, 48_ first guide plate, 49_ second guide plate, 410_ first push block, 411_ second push block, 412_ second guide plate, 413_ guide spring, 5_ drive assembly, 51_ bevel frame, 52_ guide bar, 53_ guide bar, 54_ first compression spring, 55_ bevel bar, 56_ second compression spring, 57_ pull plate, 58_ slotted guide plate, 59_ drive rack, 510_ third compression spring, 511_ bevel strip, 512_ drive gear, 6_ support assembly, 61_ support plate, 62_ support bar, 63_ support spring, 64_ separation bar, 7_ oxygen injection assembly, 71_ lower pressure plate, 72_ homing spring, 73_ movable plate, 74_ fourth compression spring, 75_ gas injection tube.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further explained below by combining the specific drawings.
Example 1
The utility model provides a nutrient solution adds equipment regularly with equivalent suitable for microorganism breeds cultivation, as shown in fig. 1-11, including bottom plate 1, place subassembly 2, culture dish subassembly 3, nutrient solution extraction subassembly 4, drive assembly 5 and supporting component 6, be equipped with on the bottom plate 1 and place subassembly 2, be equipped with culture dish subassembly 3 on placing subassembly 2, be equipped with nutrient solution extraction subassembly 4 on the bottom plate 1, nutrient solution extraction subassembly 4 is used for extracting quantitative nutrient solution, be equipped with drive assembly 5 on the bottom plate 1, drive assembly 5 is used for making the position of four culture dish bodies 31 change, be equipped with supporting component 6 on the drive assembly 5.
The placing component 2 comprises a rotating shaft 21 and a placing disc 22, the rotating shaft 21 is rotatably connected on the bottom plate 1, and the placing disc 22 is connected at the top end of the rotating shaft 21.
The culture dish assembly 3 comprises a culture dish body 31, a first guide plate 32, an inclined plane sealing plate 33 and fixing springs 34, the four culture dish bodies 31 are placed in a distributed mode on the top of the placing tray 22, the first guide plate 32 is connected to the culture dish body 31, the inclined plane sealing plate 33 is symmetrically connected to the first guide plate 32 in a sliding mode, the two inclined plane sealing plates 33 are in contact, the two fixing springs 34 are connected between the inclined plane sealing plate 33 and the first guide plate 32, and the fixing springs 34 are used for driving the inclined plane sealing plate 33 to reset.
The nutrient solution extraction component 4 comprises a support frame 41, an electric push rod 42, a guide frame 43, a movable bar 44, a return spring 45, a rubber sleeve 46, a support frame 47, a first guide plate 48, a second guide plate 49, a first push block 410, a second push block 411, a second guide plate 412 and a guide spring 413, wherein the support frame 41 is connected to the top of the bottom plate 1, the electric push rod 42 is installed on the support frame 41, the electric push rod 42 is used for driving the guide frame 43 and the upper device thereof to reciprocate, one end of the electric push rod 42 is connected with the guide frame 43, the movable bar 44 is connected with the guide frame 43 in a sliding manner on the guide frame 43, the return spring 45 is connected between the movable bar 44 and the guide frame 43, the movable bar 44 used for returning the upper device thereof, the rubber sleeve 46 is connected to the movable bar 44, the support frame 47 is connected to the support frame 41, the first guide plate 48 is connected to the support frame 47, the first guide plate 48 is connected with the movable bar 44 in a sliding manner, the second guide plate 49 is coupled to the supporting plate frame 47, the first pushing block 410 is coupled to the second guide plate 49, the second pushing block 411 is coupled to the first guide plate 48, the two second guide plates 412 are slidably coupled to the second guide plate 49, the movable bar 44 and the upper device thereof can move along the sliding rail between the first guide plate 48 and the second guide plate 49 by the cooperation of the first guide plate 48, the second guide plate 49 and the second guide plate 412, the guide spring 413 is connected between the second guide plate 412 and the second guide plate 49, and the guide spring 413 is used for driving the second guide plate 412 to return.
The driving component 5 comprises a bevel frame 51, a guide slat 52, a guide rod 53, a first compression spring 54, a bevel lever 55, a second compression spring 56, a pull plate 57, a slotted guide plate 58, a driving rack 59, a third compression spring 510, a bevel bar 511 and a driving gear 512, the bevel frame 51 is connected with the top of the bottom plate 1, the bevel frame 51 is used for extruding the pull plate 57 and moving the devices thereon, the guide slat 52 is placed on the top of the bottom plate 1, the guide rod 53 is connected with the guide slat 52, the guide rod 53 is connected with the bevel frame 51 in a sliding way, the first compression spring 54 is connected between the bevel frame 51 and the guide slat 52, the first compression spring 54 is used for driving the guide slat 52 and resetting the devices thereon, the bevel lever 55 is connected with the guide slat 52 in a sliding way, the second compression spring 56 is connected between the bevel lever 55 and the guide slat 52, the second compression spring 56 is used for driving the bevel lever 55 to reset, one end of the inclined rod 55 is connected with a pulling plate 57, the guide strip plate 52 is connected with a slotted guide plate 58, the slotted guide plate 58 is connected with a driving rack 59 in a sliding manner, the driving rack 59 is used for driving a driving gear 512 and an upper device thereof to rotate reversely for a quarter of a circle, a third compression spring 510 is connected between the driving rack 59 and the slotted guide plate 58, the third compression spring 510 is used for driving the driving rack 59 to reset, the top of the bottom plate 1 is connected with an inclined strip 511, the inclined strip 511 is used for extruding the driving rack 59 to move upwards, and the lower part of the rotating shaft 21 is connected with the driving gear 512.
The supporting assembly 6 comprises a supporting plate 61, a supporting bar 62, a supporting spring 63 and a separating rod 64, wherein the supporting plate 61 is connected to one side of the slotted guide plate 58, the supporting bar 62 is slidably connected to the supporting plate 61, the supporting spring 63 is connected between the supporting bar 62 and the supporting plate 61, the supporting spring 63 is used for driving the supporting bar 62 and devices thereon to move, the separating rod 64 is connected to the supporting frame 41, the separating rod 64 is used for pressing the supporting bar 62 to reset, and the separating rod 64 is in contact with the supporting bar 62.
A nutrient solution containing groove is arranged on the bottom plate 1 below the first guide plate 48, nutrient solution is contained in the nutrient solution containing groove, a rubber head dropper is sleeved on the rubber sleeve 46, the same amount of culture solution is contained in the culture dish body 31, the electric push rod 42 is manually controlled to contract, so that the guide frame 43 and the device on the guide frame move towards the direction close to the support frame 41, the movable strip 44 and the device on the movable strip move along the sliding rail below the first guide plate 48 and the second guide plate 49 through the matching of the first guide plate 48 and the second guide plate 49 with one of the second guide plates 412, in the process, the first push block 410 can extrude the rubber head dropper on the rubber sleeve 46, so that the air pressure inside the rubber head dropper is smaller than the external air pressure, because the rubber head dropper on the rubber sleeve 46 is in contact with the nutrient solution in the nutrient solution containing groove, when the rubber head dropper on the rubber sleeve 46 is separated from the first block 410, the rubber dropper on the rubber sleeve 46 is restored, so that the rubber dropper on the rubber sleeve 46 can suck a certain amount of nutrient solution.
Since the return spring 45 is compressed, then the movable bar 44 and the device thereon move upward under the action of the return spring 45, the movable bar 44 pushes the other second guide plate 412 to move, the other guide spring 413 is compressed, then the movable bar 44 contacts with the other side of the other second guide plate 412, the other second guide plate 412 is reset under the action of the other guide spring 413, then the movable bar 44 and the device thereon move along the first guide plate 48, the rubber head dropper on the rubber sleeve 46 presses one of the pair of inclined sealing plates 33 to move away from each other, and the one pair of fixed springs 34 is compressed, so that the rubber head dropper on the rubber sleeve 46 can enter into one of the culture dish bodies 31, then the rubber head dropper on the rubber sleeve 46 contacts with the second pushing block 411, and the second pushing block 411 presses the rubber head dropper on the rubber sleeve 46, so that the nutrient solution sucked in the rubber head dropper on the rubber sleeve 46 can be dripped into one of the culture dish bodies 31, and the effect of automatically adding quantitative nutrient solution is achieved.
Then the electric push rod 42 is manually controlled to extend, so that the guide frame 43 and the devices thereon are reset, the movable bar 44 and the devices thereon are reset along the upper slide rail between the first guide plate 48 and the second guide plate 49, in the process, the movable bar 44 pushes one of the second guide plates 412 to move, one of the guide springs 413 is compressed accordingly, then the movable bar 44 moves to the other side of one of the second guide plates 412, one of the second guide plates 412 is reset under the reset action of one of the guide springs 413, and the reset spring 45 is compressed and reset simultaneously.
When the movable strip 44 and the rubber dropper thereon are reset, the rubber dropper on the rubber sleeve 46 is separated from the pair of inclined sealing plates 33, and the pair of inclined sealing plates 33 are reset under the reset action of the pair of fixed springs 34.
During the process that the guiding frame 43 and the device thereon move towards the direction approaching the supporting frame 41, the guiding frame 43 will push the incline rod 55 and the device thereon to move, the second compression spring 56 will be compressed accordingly, then the guiding frame 43 will move to the other side of the incline rod 55, the incline rod 55 and the device thereon will be reset by the reset action of the second compression spring 56, during the process of resetting the guiding frame 43 and the device thereon, the guiding frame 43 will drive the inclined rod 55 and the device thereon to move away from the supporting frame 41, and the first compression spring 54 will be compressed accordingly, in the process, the driving rack 59 drives the driving gear 512 and the device thereon to rotate reversely for a quarter of a turn, so that the positions of the four culture dish bodies 31 can be changed, and at this time, the rubber dropper on the rubber sleeve 46 is separated from one of the pair of inclined sealing plates 33, so that a certain amount of nutrient solution can be conveniently added to the next culture dish body 31.
When the slotted guide plate 58 and the upper device thereof move away from the support frame 41, the support bar 62 is separated from the separation rod 64, the stretched support spring 63 is restored, so that the support bar 62 and the upper device thereof move, then the drive rack 59 is contacted with the inclined plane strip 511, the inclined plane strip 511 presses the drive rack 59 to move upwards, the third compression spring 510 is compressed, in the process, the drive rack 59 pushes the support bar 62 to move, the support spring 63 is stretched, then the drive rack 59 is contacted with the upper part of the support bar 62, the support bar 62 is reset under the reset action of the support spring 63, so that the support bar 62 clamps the drive rack 59, the drive rack 59 is prevented from resetting, then the pull plate 57 is contacted with the inclined plane frame 51, the inclined plane frame 51 presses the pull plate 57 and the upper device thereof, and the second compression spring 56 is compressed, so that the inclined plane rod 55 is separated from the guide frame 43, the inclined plane rod 55 and the device thereon are reset under the reset action of the first compression spring 54, in the process, the driving rack 59 is clamped by the supporting bar 62, so that the driving rack 59 is not contacted with the driving gear 512, then the separating rod 64 is pressed by the supporting bar 62 to reset, the supporting spring 63 is stretched and reset, so that the supporting bar 62 is not clamped with the driving rack 59, and the driving rack 59 is reset under the reset action of the third compression spring 510.
Example 2
Based on embodiment 1, as shown in fig. 11, the culture dish further comprises an oxygen injection assembly 7, the oxygen injection assembly 7 is disposed on the support frame 41, the oxygen injection assembly 7 is used for injecting oxygen into the culture dish body 31 to which the nutrient solution is added, the oxygen injection assembly 7 comprises a lower pressing plate 71, a return spring 72, a movable plate 73, a fourth compression spring 74 and an air injection pipe 75, the lower pressing plate 71 is slidably connected to the support frame 41, the lower pressing plate 71 is used for pressing the movable plate 73 and the upper device thereof to move downward, the lower pressing plate 71 is in contact with the inclined plane 511, the return spring 72 is connected between the support frame 41 and the lower pressing plate 71, the return spring 72 is used for driving the lower pressing plate 71 to return, the movable plate 73 is slidably connected to the support frame 41, the movable plate 73 is in contact with the lower pressing plate 71, the fourth compression spring 74 is connected between the movable plate 73 and the support frame 41, the fourth compression spring 74 is used for driving the movable plate 73 and the upper device thereof to return, the gas injection pipe 75 is coupled to the movable plate 73, and the gas injection pipe 75 is used to inject oxygen into the interior of the culture dish body 31 to which the nutrient solution is added.
The gas injection pipe 75 is connected with other oxygen supply ends, in the process that the driving rack 59 and the upper device thereof move away from the support frame 41, the driving rack 59 pushes the lower pressing plate 71 to move, the return spring 72 is compressed therewith, the lower pressing plate 71 presses the movable plate 73 and the upper device thereof to move downwards, the fourth compression spring 74 is compressed therewith, the gas injection pipe 75 can also enter one of the culture dish bodies 31 added with the nutrient solution, other oxygen supply equipment is manually controlled to inject oxygen into one of the culture dish bodies 31 added with the nutrient solution through the gas injection pipe 75, the effect of automatically injecting the oxygen into one of the culture dish bodies 31 added with the nutrient solution is achieved, in the process that the driving rack 59 and the upper device thereof are reset, the lower pressing plate 71 is reset under the reset action of the return spring 72, the movable plate 73 and the upper device thereof are reset under the reset action of the fourth compression spring 74, and manually controlling other oxygen supply equipment to stop delivering oxygen, finally manually controlling the electric push rod 42 to stop moving, and repeating the operation after one hour to add a certain amount of nutrient solution to the next culture dish body 31.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a nutrient solution adds equipment regularly with equivalent suitable for microorganism breeds cultivation, a serial communication port, including bottom plate (1), place subassembly (2), culture dish subassembly (3), nutrient solution extraction subassembly (4), drive assembly (5) and supporting component (6), be equipped with on bottom plate (1) and place subassembly (2), be equipped with culture dish subassembly (3) on placing subassembly (2), be equipped with nutrient solution extraction subassembly (4) on bottom plate (1), be equipped with drive assembly (5) on bottom plate (1), be equipped with supporting component (6) on drive assembly (5).
2. The device for adding the nutrient solution into the culture medium for the breeding of the microorganisms according to claim 1, wherein the placing component (2) comprises a rotating shaft (21) and a placing plate (22), the rotating shaft (21) is rotatably connected to the bottom plate (1), and the placing plate (22) is connected to the top end of the rotating shaft (21).
3. The timed and equivalent nutrient solution adding device suitable for the breeding culture of the microorganisms according to claim 2, wherein the culture dish assembly (3) comprises a culture dish body (31), a first guide plate (32), inclined plane sealing plates (33) and fixing springs (34), the four culture dish bodies (31) are placed on the top of the placing tray (22) in a distributed manner, the first guide plate (32) is connected to the culture dish body (31), the inclined plane sealing plates (33) are symmetrically and slidably connected to the first guide plate (32), the two inclined plane sealing plates (33) are in contact, and the two fixing springs (34) are connected between the inclined plane sealing plates (33) and the first guide plate (32).
4. The device for adding the nutrient solution at regular time and in equal quantity suitable for the breeding and the culture of the microorganisms according to claim 3, wherein the nutrient solution extracting component (4) comprises a supporting frame (41), an electric push rod (42), a guide frame (43), a movable strip (44), a return spring (45), a rubber sleeve (46), a support plate frame (47), a first guide plate (48), a second guide plate (49), a first pushing block (410), a second pushing block (411), a second guide plate (412) and a guide spring (413), the supporting frame (41) is connected to the top of the bottom plate (1), the electric push rod (42) is installed on the supporting frame (41), the guide frame (43) is connected to one end of the electric push rod (42), the movable strip (44) is connected to the guide frame (43) in a sliding manner, the return spring (45) is connected between the movable strip (44) and the guide frame (43), the rubber sleeve (46) is connected to the movable strip (44), the supporting frame (47) is connected to the supporting frame (41), the first guide plate (48) is connected to the supporting frame (47), the first guide plate (48) is connected with the movable strip (44) in a sliding mode, the second guide plate (49) is connected to the supporting frame (47), the first pushing block (410) is connected to the second guide plate (49) in a connecting mode, the second pushing block (411) is connected to the first guide plate (48) in a connecting mode, the two second guide plates (412) are connected to the second guide plate (49) in a sliding mode, and the guide spring (413) is connected between the second guide plates (412) and the second guide plates (49).
5. The device for adding the nutrient solution with equal amount at regular time suitable for the breeding and the cultivation of the microorganism as the claim 4, which is characterized in that the driving component (5) comprises an inclined plane frame (51), a guide strip plate (52), a guide rod (53), a first compression spring (54), an inclined plane rod (55), a second compression spring (56), a pulling plate (57), a slotted guide plate (58), a driving rack (59), a third compression spring (510), an inclined plane strip (511) and a driving gear (512), wherein the top of the bottom plate (1) is connected with the inclined plane frame (51), the guide strip plate (52) is placed at the top of the bottom plate (1), the guide rod (53) is connected with the guide strip plate (52) in a sliding way, the first compression spring (54) is connected between the inclined plane frame (51) and the guide strip (52), the inclined plane rod (55) is connected with the guide strip (52) in a sliding way, a second compression spring (56) is connected between the inclined rod (55) and the guide strip plate (52), one end of the inclined rod (55) is connected with a pulling plate (57), the guide strip plate (52) is connected with a slotting guide plate (58), the slotting guide plate (58) is connected with a driving rack (59) in a sliding mode, a third compression spring (510) is connected between the driving rack (59) and the slotting guide plate (58), the top of the bottom plate (1) is connected with an inclined noodle strip (511), and the lower part of the rotating shaft (21) is connected with a driving gear (512).
6. The device for timed and equivalent addition of nutrient solution suitable for breeding and culturing of microorganisms according to claim 5, wherein the support assembly (6) comprises a support plate (61), a support bar (62), a support spring (63) and a separation rod (64), the support plate (61) is connected to one side of the slotted guide plate (58), the support bar (62) is slidably connected to the support plate (61), the support spring (63) is connected between the support bar (62) and the support plate (61), the separation rod (64) is connected to the support frame (41), and the separation rod (64) is in contact with the support bar (62).
7. A device for the timed and equivalent addition of nutrient solution for the breeding culture of microorganisms according to claim 6, the novel oxygen injection device is characterized by further comprising an oxygen injection assembly (7), wherein the oxygen injection assembly (7) is arranged on the support frame (41), the oxygen injection assembly (7) comprises a lower pressing plate (71), a homing spring (72), a movable plate (73), a fourth compression spring (74) and an air injection pipe (75), the lower pressing plate (71) is connected to the support frame (41) in a sliding mode, the lower pressing plate (71) is in contact with the inclined noodles (511), the homing spring (72) is connected between the support frame (41) and the lower pressing plate (71), the movable plate (73) is connected to the support frame (41) in a sliding mode, the movable plate (73) is in contact with the lower pressing plate (71), the fourth compression spring (74) is connected between the movable plate (73) and the support frame (41), and the air injection pipe (75) is connected to the movable plate (73).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110881139.1A CN113621478A (en) | 2021-08-02 | 2021-08-02 | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110881139.1A CN113621478A (en) | 2021-08-02 | 2021-08-02 | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113621478A true CN113621478A (en) | 2021-11-09 |
Family
ID=78382264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110881139.1A Pending CN113621478A (en) | 2021-08-02 | 2021-08-02 | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113621478A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114058488A (en) * | 2021-12-15 | 2022-02-18 | 肖艳平 | Strain culture device convenient to coat based on enzyme engineering |
| CN114410451A (en) * | 2022-01-17 | 2022-04-29 | 罗志勇 | Tumor cell culture device for oncology department |
| CN116676169A (en) * | 2023-08-03 | 2023-09-01 | 山东水发生命科学研究有限公司 | Stem cell culture device and use method thereof |
| CN117165427A (en) * | 2023-11-03 | 2023-12-05 | 昆明建新园食品销售有限公司 | Sauce microorganism detection device for marinating pork and detection method thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08173141A (en) * | 1994-12-28 | 1996-07-09 | Kirin Brewery Co Ltd | Device for dividedly injecting medium |
| WO2013129558A1 (en) * | 2012-02-29 | 2013-09-06 | 株式会社ジェイテック | Cell culture device having culture medium replacement function |
| CN207699596U (en) * | 2017-12-18 | 2018-08-07 | 深圳市泰华细胞工程有限公司 | A kind of cell biological culture dish |
| CN210261800U (en) * | 2019-07-12 | 2020-04-07 | 哈尔滨商业大学 | Make things convenient for microbial cultivation case of nutrient solution equipartition |
| CN210419992U (en) * | 2019-08-16 | 2020-04-28 | 赵雨轩 | Cell culture device |
| CN111575155A (en) * | 2020-05-31 | 2020-08-25 | 常开旭 | Automatic add microbial cultivation ware of liquid medium |
| CN212476700U (en) * | 2020-05-26 | 2021-02-05 | 河南中科干细胞基因工程有限公司 | Culture solution homogenizing and filling equipment for cell culture |
| CN112457980A (en) * | 2020-11-24 | 2021-03-09 | 湖北军翔信息技术有限公司 | A culture dish that can add culture solution automatically for medical experiment |
| CN112574873A (en) * | 2020-12-21 | 2021-03-30 | 郭向东 | Culture dish capable of automatically supplying nutrient solution for biological experiment |
| CN112760223A (en) * | 2021-01-22 | 2021-05-07 | 宋铁英 | Stem cell culture system and culture method thereof |
| CN213456246U (en) * | 2020-09-10 | 2021-06-15 | 蒋石艳 | Medical treatment clinical laboratory uses reagent sample operation panel |
-
2021
- 2021-08-02 CN CN202110881139.1A patent/CN113621478A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08173141A (en) * | 1994-12-28 | 1996-07-09 | Kirin Brewery Co Ltd | Device for dividedly injecting medium |
| WO2013129558A1 (en) * | 2012-02-29 | 2013-09-06 | 株式会社ジェイテック | Cell culture device having culture medium replacement function |
| CN207699596U (en) * | 2017-12-18 | 2018-08-07 | 深圳市泰华细胞工程有限公司 | A kind of cell biological culture dish |
| CN210261800U (en) * | 2019-07-12 | 2020-04-07 | 哈尔滨商业大学 | Make things convenient for microbial cultivation case of nutrient solution equipartition |
| CN210419992U (en) * | 2019-08-16 | 2020-04-28 | 赵雨轩 | Cell culture device |
| CN212476700U (en) * | 2020-05-26 | 2021-02-05 | 河南中科干细胞基因工程有限公司 | Culture solution homogenizing and filling equipment for cell culture |
| CN111575155A (en) * | 2020-05-31 | 2020-08-25 | 常开旭 | Automatic add microbial cultivation ware of liquid medium |
| CN213456246U (en) * | 2020-09-10 | 2021-06-15 | 蒋石艳 | Medical treatment clinical laboratory uses reagent sample operation panel |
| CN112457980A (en) * | 2020-11-24 | 2021-03-09 | 湖北军翔信息技术有限公司 | A culture dish that can add culture solution automatically for medical experiment |
| CN112574873A (en) * | 2020-12-21 | 2021-03-30 | 郭向东 | Culture dish capable of automatically supplying nutrient solution for biological experiment |
| CN112760223A (en) * | 2021-01-22 | 2021-05-07 | 宋铁英 | Stem cell culture system and culture method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114058488A (en) * | 2021-12-15 | 2022-02-18 | 肖艳平 | Strain culture device convenient to coat based on enzyme engineering |
| CN114058488B (en) * | 2021-12-15 | 2023-10-13 | 涌源合生科技(深圳)有限公司 | Strain culture device based on enzyme engineering is convenient for coating |
| CN114410451A (en) * | 2022-01-17 | 2022-04-29 | 罗志勇 | Tumor cell culture device for oncology department |
| CN116676169A (en) * | 2023-08-03 | 2023-09-01 | 山东水发生命科学研究有限公司 | Stem cell culture device and use method thereof |
| CN116676169B (en) * | 2023-08-03 | 2023-10-27 | 山东水发生命科学研究有限公司 | Stem cell culture device and use method thereof |
| CN117165427A (en) * | 2023-11-03 | 2023-12-05 | 昆明建新园食品销售有限公司 | Sauce microorganism detection device for marinating pork and detection method thereof |
| CN117165427B (en) * | 2023-11-03 | 2024-02-20 | 昆明建新园食品销售有限公司 | Sauce microorganism detection device for marinating pork and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113621478A (en) | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture | |
| CN212199289U (en) | Cell culture device | |
| CN111218394B (en) | Reduce bacterial solution inoculation device that other bacterial sneaked into | |
| CN107828636B (en) | Microalgae breeding device | |
| CN112616572B (en) | White beech mushroom culture device and culture method | |
| CN211881344U (en) | Forestry seedling raising box | |
| CN110972942B (en) | Intelligent seedling breeding method | |
| CN208402717U (en) | A kind of mushroom liquid bacterial breeding apparatus | |
| CN115305202A (en) | Microbial culture process and equipment for green agriculture | |
| CN211532248U (en) | Intelligent seedling breeding system | |
| CN205161221U (en) | Sea fresh mushroom's cultivation inoculation device | |
| CN208800517U (en) | A kind of medicinal material tissue culture bottle culture medium cleaning plant | |
| KR20230090369A (en) | Automated cell culture device | |
| CN207443751U (en) | A kind of liquid spawn inoculator | |
| CN107996290B (en) | Device for filling edible fungus culture material and application method thereof | |
| CN208191627U (en) | A kind of edible fungus species breeding apparatus | |
| CN108271472B (en) | Batch nature vegetable seed is scattered and is used equipment | |
| CN108260382B (en) | Batch vegetable seed is scattered and is used equipment with buffer function | |
| CN116144469B (en) | Bifidobacterium culture equipment | |
| CN110800614A (en) | Intelligent seedling breeding system | |
| CN2659126Y (en) | Auto forming machine for turf seedlings growth disc | |
| CN220557012U (en) | Fruit planting incubator convenient to use | |
| CN217363963U (en) | Wheat breeding device | |
| CN220812459U (en) | Multilayer microalgae growth and reproduction device | |
| CN220255068U (en) | Potted seed sowing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |