CN114058488B - Strain culture device based on enzyme engineering is convenient for coating - Google Patents
Strain culture device based on enzyme engineering is convenient for coating Download PDFInfo
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- CN114058488B CN114058488B CN202111532768.XA CN202111532768A CN114058488B CN 114058488 B CN114058488 B CN 114058488B CN 202111532768 A CN202111532768 A CN 202111532768A CN 114058488 B CN114058488 B CN 114058488B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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Abstract
The invention relates to the field of enzyme engineering, in particular to a strain culture device which is convenient to coat and is based on enzyme engineering, and the strain culture device comprises a support base, a first support frame, a second support frame, a first rotating frame and the like; the support base is connected with a first support frame through a bolt, the top of the first support frame is connected with a second support frame, and the first support frame is connected with a first rotating frame in a rotating mode. The second rotating frame can be clung to the surface of the agar culture medium and rotate, so that bacterial liquid on the surface of the agar culture medium can be uniformly coated, bacterial can be conveniently cultured, the agar culture medium can be prevented from being damaged, and the effects of automatically and efficiently coating bacterial liquid on the surface of the agar culture medium uniformly and ensuring the integrity of the agar culture medium are achieved.
Description
Technical Field
The invention relates to the field of enzyme engineering, in particular to a strain culture device which is convenient to coat and is based on enzyme engineering.
Background
In laboratory conditions, a method for artificially growing and reproducing a large amount of pure strains is called strain culture, and in the research of mildew-proof and antibacterial, it is generally required to separate and culture the microorganisms causing mildew and rot, and separate different species of microorganisms from a mixed microorganism population to obtain pure species. There are many methods for obtaining pure species, but the basic principle is similar, namely, the isolated sample is diluted to a certain extent so that the cells of the microorganism exist in a dispersed state as much as possible, then single colonies are grown, and then the single colonies are transferred to a proper culture medium, so that pure species of the microorganism are generally obtained.
In the process of strain culture, bacterial liquid needs to be coated on a culture medium, in the prior art, the bacterial liquid is manually injected into the culture medium, the bacterial liquid is immediately and evenly coated by a coating rod, namely, a left hand holds a culture dish, a slit is opened on a dish cover, and a right hand holds the coating rod to move on the surface of the culture medium so as to achieve the effect of coating the bacterial liquid, but the manual operation of the prior art is easy to be mistaken, so that the culture medium is pushed to be broken, the strain culture is not facilitated, and in addition, when the bacterial liquid is manually injected, the dish cover needs to be opened, so that bacteria are easy to enter the inside of the culture dish, and the strain culture is influenced.
Disclosure of Invention
In view of the above, it is necessary to provide a strain culture device which can automatically and efficiently coat a bacterial liquid on the surface of an agar medium uniformly, ensure the integrity of the agar medium, and maintain a closed culture environment of a culture dish, is convenient to coat based on enzyme engineering, so as to solve the problems that the prior art manual operation is easy to cause the pushing of the culture medium due to errors and the bacteria are easy to enter the interior of the culture dish.
The technical implementation scheme of the invention is as follows: the utility model provides a strain culture apparatus based on coating of being convenient for of enzyme engineering, is including supporting base, first support frame, second support frame, first rotating frame, placing frame, spacing, culture dish, support apron, first spring, coating subassembly and hugging closely the subassembly:
the first support frame is connected with the support base through bolts and is of a circular structure;
the top of the first support frame is connected with the second support frame;
the first rotating frame is rotatably connected with the first supporting frame;
the first rotating frame is fixedly connected with a placing frame which adopts a circular structure;
the top of the placement frame is connected with a limiting frame;
the culture dish is placed on the placement frame and is used for culturing strains;
the limiting frame is connected with a support cover plate in a sliding mode, the support cover plate is rectangular, and the support cover plate is used for covering the culture dish;
the first spring is connected between the supporting cover plate and the limiting frame;
the coating component is arranged on the supporting cover plate and used for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium;
the coating component is provided with a clinging component, and the clinging component is used for clinging the coating component to the surface of the agar culture medium.
More preferably, the coating assembly comprises an arc-shaped rack, a first limit column, a sliding support plate, a rotary slotted plate, a second spring, a second rotating frame, a third spring, a rotary support ring and a spur gear, wherein the arc-shaped rack is connected to the second support frame, the top of the support cover plate is symmetrically connected with the first limit column, the sliding support plate is connected between the two first limit columns in a sliding mode, the rotary slotted plate is connected inside the sliding support plate in a rotating mode, the second spring is symmetrically connected between the sliding support plate and the support cover plate, the second rotating frame is connected to the rotary slotted plate in a sliding mode, the third spring is connected between the second rotating frame and the rotary slotted plate, the rotary support ring is matched with the second rotating frame in a sliding mode, and the spur gear is connected to the top of the rotary support ring.
More preferably, the lower part of the second rotating frame adopts a straight structure for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium.
More preferably, the cling assembly comprises a pushing frame and an arc limiting plate, the top of the sliding support plate is connected with the pushing frame, and the second support frame is connected with the arc limiting plate.
More preferably, the anti-sputtering device further comprises an anti-sputtering assembly, wherein the anti-sputtering assembly is arranged on the first support frame, the anti-sputtering assembly comprises a third support frame, a pushing plate, a fourth spring, a support rod, an arc-shaped grooving support plate, a limiting sliding frame, a sliding support frame, a fifth spring, a one-way ingress pipe, an arc-shaped extrusion plate, a sixth spring and a reset frame, the top of the support cover plate is connected with the third support frame, the pushing plate is connected with the sliding on the third support frame, the fourth spring is connected between the pushing plate and the third support frame, the support rod is connected with the first support frame, one end of the support rod is connected with the arc-shaped grooving support plate, the limiting sliding frame is connected with the sliding support frame in a sliding mode, the fifth spring is connected between the sliding support frame and the limiting sliding support frame, the one-way ingress pipe is connected with the arc-shaped extrusion plate on the support rod, the arc-shaped extrusion plate is contacted with the sliding support frame, the sixth spring is connected between the arc-shaped grooving support plate and the limiting sliding support frame, and the reset frame is connected with the reset frame on the support rod.
More preferably, the device further comprises an extrusion assembly, the extrusion assembly is arranged on the anti-sputtering assembly, the extrusion assembly comprises a fourth support frame, a push plate frame, a seventh spring, a fifth support frame, a liquid storage frame, a threaded cover, a one-way guide pipe and a wedge-shaped extrusion block, the fourth support frame is connected to the top of the sliding support frame, the push plate frame is connected to the inside of the sliding support frame in a sliding mode, the seventh spring is connected between the push plate frame and the fourth support frame, the fifth support frame is connected to the limiting sliding frame, the liquid storage frame is connected to the top of the fifth support frame, the threaded cover is connected to the top of the liquid storage frame through threads, the one-way guide pipe is communicated to the bottom of the liquid storage frame, the one-way guide pipe is communicated with the one-way guide pipe, and the wedge-shaped extrusion block is symmetrically connected to one side of the arc-shaped extrusion plate.
More preferably, the device further comprises a sealing component, wherein the sealing component is arranged on the supporting cover plate and comprises a sliding perforated frame, an eighth spring, a sliding partition plate frame and a guide groove frame, the sliding perforated frame is connected to the top of the supporting cover plate in a sliding mode, the eighth spring is connected between the sliding perforated frame and the supporting cover plate, the sliding partition plate frame is connected to the bottom of the sliding perforated frame in a sliding mode, the guide groove frame is connected to the bottom of the supporting cover plate in a connecting mode, and the guide groove frame is in limiting fit with the sliding partition plate frame.
More preferably, the device further comprises an opening and closing assembly, wherein the opening and closing assembly is arranged on the supporting cover plate and comprises a second limiting column and an arc inclined plate, the supporting cover plate is connected with the second limiting column, the arc inclined plate is fixedly connected to the first supporting frame, and the arc inclined plate is in contact with the second limiting column.
Compared with the prior art, the invention has the following advantages:
the second rotating frame can be clung to the surface of the agar culture medium and rotate, so that bacterial liquid on the surface of the agar culture medium can be uniformly coated, bacterial can be conveniently cultured, the agar culture medium can be prevented from being damaged, and the effects of automatically and efficiently coating bacterial liquid on the surface of the agar culture medium uniformly and ensuring the integrity of the agar culture medium are achieved.
The unidirectional ingress pipe and the device thereon can move downwards and be inserted into the support cover plate, so that the unidirectional ingress pipe is close to the surface of the agar culture medium, and is convenient for fully and completely injecting the bacterial liquid to the surface of the agar culture medium, thereby realizing the purpose of effectively preventing the bacterial liquid from being sprayed.
The unidirectional ingress pipe can draw the bacterial liquid and pour it into the agar culture medium surface, has offered convenience for culturing the bacterial, has reached the effect that can pour the bacterial liquid into the agar culture medium surface automatically.
In the process of injecting the bacterial liquid into the surface of the agar culture medium, the sliding partition plate frame can be opened, and after the injection is finished, the sliding partition plate frame can be immediately closed, so that the closed culture environment of the culture dish is conveniently maintained, the strain in the culture dish is prevented from being polluted by the external environment, and the normal growth and propagation of the strain in the culture dish are facilitated.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention.
Fig. 2 is a schematic perspective view of a second embodiment of the present invention.
Fig. 3 is a schematic perspective view of a close-fitting assembly according to the present invention.
Fig. 4 is a partially disassembled perspective view of the coating assembly of the present invention.
Fig. 5 is a schematic perspective view of a first part of the present invention.
Fig. 6 is a schematic perspective view of a first part of the sputtering prevention assembly of the present invention.
Fig. 7 is a schematic perspective view of a second part of the present invention.
Fig. 8 is a schematic view of a second partial perspective view of the sputter preventing assembly of the present invention.
Fig. 9 is a schematic perspective view of a third portion of the present invention.
Fig. 10 is a schematic perspective view of a third portion of the sputter preventing assembly of the present invention.
FIG. 11 is a schematic view of a portion of a push-in assembly of the present invention in perspective.
Fig. 12 is a schematic perspective view of a fourth portion of the present invention.
Fig. 13 is a schematic view showing a partial perspective structure of the closure assembly of the present invention.
Fig. 14 is a schematic perspective view of a closure assembly according to the present invention.
Fig. 15 is a schematic perspective view of an opening and closing assembly according to the present invention.
The marks of the components in the drawings are as follows: 1. the support base, 22, the first support frame, 23, the second support frame, 24, the first rotating frame, 25, the placing frame, 26, the limit frame, 261, the culture dish, 27, the support cover plate, 28, the first spring, 3, the coating component, 31, the arc rack, 32, the first limit post, 33, the sliding support plate, 331, the rotating slotted plate, 34, the second spring, 35, the second rotating frame, 36, the third spring, 37, the rotating support ring, 38, the spur gear, 4, the clinging component, 41, the pushing frame, 42, the arc limit plate, 5, the anti-sputtering component, 51, the third support frame, 52, the pushing plate, 53, the fourth spring, 54, the support rod, 55, the arc slotted support plate, 56, the limit sliding frame, 57, the sliding support frame, 571, the fifth spring, 58, the unidirectional ingress pipe, 59, the arc extrusion plate, 510, the sixth spring, 511, the resetting frame, 6, the extrusion component, 61, the fourth support frame, 62, the push plate frame, 63, the seventh spring, 64, the fifth support frame, 65, the liquid storage frame, 66, the guide plate, 66, 67, the wedge-shaped component, the arc opening and closing component, the arc frame, the arc opening and closing component, and closing.
Detailed Description
Embodiments of the present invention are described in further detail below with reference to the accompanying drawings and examples. The following examples are illustrative of the invention but are not intended to limit the scope of the invention.
Example 1
The utility model provides a strain culture device convenient to coat based on enzyme engineering, as shown in fig. 1-5, including supporting base 1, first support frame 22, second support frame 23, first revolving frame 24, place frame 25, spacing 26, culture dish 261, supporting cover plate 27, first spring 28, the coating subassembly 3 and hug closely subassembly 4, supporting base 1 is circular structure, there is first support frame 22 through bolted connection on supporting base 1, first support frame 22 top hookup has second support frame 23, second support frame 23 is N type structure, first support frame 22 is last to be connected with first revolving frame 24 in the rotation, fixedly connected with places frame 25 on the first revolving frame 24, place frame 25 adopts circular structure, place frame 25 top hookup has spacing 26, place and place the culture dish 261 that is used for cultivating the strain on the frame 25, be connected with supporting cover plate 27 on the spacing 26 in the slip, be the rectangle supporting cover plate 27, be connected with first spring 28 between supporting cover plate 27, be equipped with on the supporting cover plate 27 and be used for will drip on agar medium surface's the even coating subassembly 3 of fungus liquid coating, be equipped with on the coating subassembly 3 that makes the coating subassembly 3 hug closely the surface of agar medium.
The coating component 3 comprises an arc-shaped rack 31, a first limit column 32, a sliding support plate 33, a rotary slotted plate 331, a second spring 34, a second rotating frame 35, a third spring 36, a rotary support ring 37 and a straight gear 38, wherein the arc-shaped rack 31 is connected to the second support frame 23, the arc-shaped rack 31 adopts an arc-shaped structure, the top of the support cover plate 27 is symmetrically connected with the first limit column 32, the sliding support plate 33 is connected between the two first limit columns 32 in a sliding mode, the rotary slotted plate 331 is rotatably connected inside the sliding support plate 33, the second spring 34 is symmetrically connected between the sliding support plate 33 and the support cover plate 27, the rotary slotted plate 331 is slidably connected with the second rotating frame 35 for uniformly coating bacterial liquid dropped on the surface of an agar culture medium, the bottom of the second rotating frame 35 is in a straight-shaped structure, the third spring 36 is connected between the second rotating frame 35 and the rotary slotted plate 331, the rotary support ring 37 is rotatably connected to the support cover plate 27, the rotary support ring 37 is slidably matched with the second rotating frame 35, and the straight gear 38 is connected to the top of the rotary support ring 37.
The close fitting assembly 4 comprises a pushing frame 41 and an arc limiting plate 42, the top of the sliding supporting plate 33 is connected with the pushing frame 41, the pushing frame 41 adopts an N-type structure, the second supporting frame 23 is connected with the arc limiting plate 42, and the arc limiting plate 42 is arc-shaped.
When the device is used, the support cover plate 27 and the upper device thereof are manually pushed to move upwards, the first spring 28 is compressed along with the support cover plate 27, sterilized agar culture medium is manually poured into the culture dish 261, the support cover plate 27 is loosened, the first spring 28 is reset along with the support cover plate 27 and the upper device thereof, so that the support cover plate 27 covers the culture dish 261, external bacteria are prevented from affecting the agar culture medium in the culture dish 261, bacteria liquid is manually dripped onto the surface of the agar culture medium on the culture dish 261 through small holes in the support cover plate 27, the placement frame 25 is manually rotated clockwise, the placement frame 25 and the upper device thereof are rotated clockwise, the pushing frame 41 is contacted with the arc-shaped limiting plate 42 in the process, the arc-shaped limiting plate 42 can press the pushing frame 41 and the upper device thereof to move downwards, the second spring 34 is compressed along with the arc-shaped limiting plate, when the second rotating frame 35 is contacted with the agar culture medium in the culture dish 261, the third spring 36 is stretched along with the support cover plate, the second rotating frame 35 is tightly attached to the agar culture medium in the culture dish 261, then the gear 38 is directly contacted with the rack 31, the reset gear 31 is driven by the reset frame 31 and the reset device is driven by the reset device, the reset along with the arc-shaped limiting plate 41, the arc-shaped limiting plate is evenly, the second rotating frame is driven by the reset device is driven by the reset, and the reset device is coated with the arc-shaped limiting plate 41, and the second rotating the agar culture medium is coated on the arc-shaped culture medium, and the upper surface is uniformly, and the reset on the upper surface of the culture medium 31.
Example 2
On the basis of embodiment 1, as shown in fig. 5-10, the device further comprises a splash-proof assembly 5, wherein the splash-proof assembly 5 for preventing the fungus liquid from splashing in the injection process is arranged on the first support frame 22, the splash-proof assembly 5 comprises a third support frame 51, a push plate 52, a fourth spring 53, a support rod 54, an arc-shaped slotted support plate 55, a limiting sliding frame 56, a sliding support frame 57, a fifth spring 571, a one-way ingress pipe 58, an arc-shaped extrusion plate 59, a sixth spring 510 and a reset frame 511, the top of the support cover plate 27 is connected with the third support frame 51, the push plate 52 is connected on the third support frame 51 in a sliding manner, a fourth spring 53 is connected between the push plate 52 and the third support frame 51, a support rod 54 is connected on the first support frame 22, one end of the support rod 54 is connected with the arc-shaped slotted support plate 55, an arc-shaped sliding groove 56 is arranged on the arc-shaped slotted support plate 55, a limiting sliding frame 57 is connected on the arc-shaped slotted support plate 55, a hollow structure is arranged inside the sliding support frame 57, a fifth spring 57 is connected between the sliding support frame 57 and the limiting sliding support frame 56, the fifth spring 57 is connected with the limiting sliding support frame 57, the bottom of the sixth spring 59 is connected with the hollow sliding support frame 57, the hollow structure is connected with the hollow support frame 59, the hollow support frame 59 is connected with the arc-shaped extrusion plate 59, the hollow support frame 59 is in a one-way, and is connected with the arc-shaped support frame 59, and is in a one side is connected with the hollow structure, and is in a hollow structure, and is connected with the hollow support frame 59.
During the rotation of the placement frame 25 and the upper device thereof, the pushing plate 52 will contact with the limit carriage 56, the pushing plate 52 will push the limit carriage 56 and the upper device thereof to move together, the sixth spring 510 will be compressed, during this process, the arc-shaped extrusion plate 59 will press the sliding support frame 57 and the upper device thereof to move downward and insert the unidirectional introducing tube 58 into the support cover plate 27, the fifth spring 571 will be compressed, then the bacteria liquid will be manually injected into the limit carriage 56, the bacteria liquid will be injected into the agar medium surface through the unidirectional introducing tube 58, the splashing of the bacteria liquid during the injection will be prevented, then the pushing plate 52 will contact with the reset frame 511, the reset frame 511 will press the pushing plate 52 to move downward and separate from the limit carriage 56, the fourth spring 53 will be compressed, the limit carriage 56 and the upper device thereof will be reset in reverse direction successively, and the bacteria liquid will be injected into the agar medium surface fully and completely.
Example 3
9-12, further include a squeezing assembly 6 for squeezing the bacterial liquid in the unidirectional introducing tube 58 to the surface of the agar medium, where the squeezing assembly 6 is disposed on the anti-splashing assembly 5, and the squeezing assembly 6 includes a fourth support frame 61, a pushing plate frame 62, a seventh spring 63, a fifth support frame 64, a liquid storage frame 65, a screw cap 66, a unidirectional flow guiding tube 67 and a wedge-shaped squeezing block 68, where the top of the sliding support frame 57 is connected with the fourth support frame 61, the sliding support frame 57 is connected with the push plate frame 62 in a sliding manner, the lower part of the push plate frame 62 is disc-shaped, a seventh spring 63 is connected between the push plate frame 62 and the fourth support frame 61, the limiting sliding frame 56 is connected with the fifth support frame 64, the top of the fifth support frame 64 is connected with the liquid storage frame 65, the top of the liquid storage frame 65 is connected with a screw cap 66 through threads, the outer bottom of the liquid storage frame 65 is connected with the unidirectional flow guiding tube 67 for transporting the bacterial liquid, the unidirectional flow guiding tube 67 is connected with the unidirectional 58, and one side of the arc-shaped squeezing plate 59 is symmetrically connected with the wedge-shaped squeezing block 68.
The liquid storage frame 65 is internally provided with a certain amount of fungus liquid, in the process of moving the limit sliding frame 56 and the device thereon, the push plate frame 62 is contacted with one wedge-shaped extrusion block 68, one wedge-shaped extrusion block 68 extrudes the push plate frame 62 downwards and extrudes air in the sliding support frame 57, the seventh spring 63 is stretched, then the push plate frame 62 is separated from one wedge-shaped extrusion block 68, the seventh spring 63 is reset and drives the push plate frame 62 to reset, the fungus liquid in the liquid storage frame 65 is pumped into the one-way ingress pipe 58 through the one-way ingress pipe 67, then the push plate frame 62 is contacted with the other wedge-shaped extrusion block 68, the other wedge-shaped extrusion block 68 extrudes the fungus liquid in the one-way ingress pipe 58 downwards and separates the push plate frame 62 from the other wedge-shaped extrusion block 68, the push plate frame 62 is reset, in the process, the one-way ingress pipe 67 and the one-way ingress pipe 58 only allow the fungus liquid to pass through in one way, the fungus liquid backflow is prevented, after the fungus liquid is injected once, the threaded cover 66 needs to be manually opened, the fungus liquid is quantitatively added into the interior of the one-way ingress pipe, and then the fungus liquid is automatically injected into the threaded cover 66, and the threaded cover is automatically repeatedly moved to the threaded surface of the fungus liquid can be manually closed.
Example 4
On the basis of embodiment 3, as shown in fig. 13-14, the culture dish further comprises a sealing component 7, the sealing component 7 for ensuring the sealed environment inside the culture dish 261 is arranged on the support cover plate 27, the sealing component 7 comprises a sliding perforated frame 71, an eighth spring 72, a sliding partition frame 73 and a guide groove frame 74, the sliding perforated frame 71 is connected with the top of the support cover plate 27 in a sliding mode, the sliding perforated frame 71 is Z-shaped, the eighth spring 72 is connected between the sliding perforated frame 71 and the support cover plate 27, the sliding partition frame 73 is connected with the bottom of the sliding perforated frame 71 in a sliding mode, the guide groove frame 74 is connected with the bottom of the support cover plate 27, an inclined chute is formed in the guide groove frame 74, and the guide groove frame 74 is in limit fit with the sliding partition frame 73.
During the downward movement of the unidirectional introducing pipe 58 and the upper device thereof, the unidirectional introducing pipe 58 can press the sliding perforated frame 71 and the upper device thereof to move downwards, the eighth spring 72 can be compressed accordingly, the sliding partition frame 73 can move along the sliding groove on the guiding groove frame 74 due to the action of the guiding groove frame 74, so that the sliding partition frame 73 is opened for injecting bacteria liquid, and when the unidirectional introducing pipe 58 and the upper device thereof are reset, the eighth spring 72 can reset and drive the sliding perforated frame 71 and the upper device thereof to reset, and the sliding partition frame 73 can be closed accordingly, so that the closed environment inside the culture dish 261 is ensured.
Example 5
On the basis of embodiment 4, as shown in fig. 15, the folding assembly 8 is further included, the folding assembly 8 is disposed on the supporting cover plate 27, the folding assembly 8 includes a second limiting column 81 and an arc inclined plate 82, the supporting cover plate 27 is connected with the second limiting column 81, the second limiting column 81 is cylindrical, the arc inclined plate 82 is fixedly connected to the first supporting frame 22, the arc inclined plate 82 adopts a double-inclined-plane structure, and the arc inclined plate 82 is in contact with the second limiting column 81.
In the process of rotating the placement frame 25 and the upper device thereof, the second limiting column 81 is separated from the arc inclined plate 82, the compressed first spring 28 is restored and drives the support cover plate 27 and the upper device thereof to move downwards, so that the support cover plate 27 is closed, then the second limiting column 81 is contacted with the arc inclined plate 82 again, the arc inclined plate 82 can extrude the second limiting column 81 and the upper device thereof to reset upwards, the first spring 28 is compressed and reset, the support cover plate 27 is opened, the support cover plate 27 is pushed to be opened instead of a human hand, and the agar culture medium is conveniently poured into the culture dish 261.
In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front," "rear," "head," "tail," and the like are used as an orientation or positional relationship based on that shown in the drawings, merely to facilitate description of the invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Claims (5)
1. A strain culture device convenient to coat based on enzyme engineering is characterized in that: including supporting base, first support frame, second support frame, first rotating frame, place frame, spacing, culture dish, support apron, first spring, coating subassembly and hugging closely the subassembly:
the first support frame is connected with the support base through bolts and is of a circular structure;
the top of the first support frame is connected with the second support frame;
the first rotating frame is rotatably connected with the first supporting frame;
the first rotating frame is fixedly connected with a placing frame which adopts a circular structure;
the top of the placement frame is connected with a limiting frame;
the culture dish is placed on the placement frame and is used for culturing strains;
the limiting frame is connected with a support cover plate in a sliding mode, the support cover plate is rectangular, and the support cover plate is used for covering the culture dish;
the first spring is connected between the supporting cover plate and the limiting frame;
the coating component is arranged on the supporting cover plate and used for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium;
the coating component is provided with a clinging component, and the clinging component is used for clinging the coating component to the surface of the agar culture medium;
the coating assembly comprises an arc-shaped rack, first limit columns, a sliding support plate, a rotary slotted plate, a second spring, a second rotating frame, a third spring, a rotary support ring and a spur gear, wherein the arc-shaped rack is connected to the second support frame, the first limit columns are symmetrically connected to the top of the support cover plate, the sliding support plates are connected between the two first limit columns in a sliding mode, the rotary slotted plate is connected to the inside of the sliding support plate in a rotating mode, the second spring is symmetrically connected between the sliding support plate and the support cover plate, the second rotating frame is connected to the rotary slotted plate in a sliding mode, the third spring is connected between the second rotating frame and the rotary slotted plate, the rotary support ring is connected to the rotary support ring in a sliding mode and matched with the second rotating frame, and the spur gear is connected to the top of the rotary support ring;
the lower part of the second rotating frame adopts a straight structure and is used for uniformly coating bacterial liquid dropped on the surface of the agar culture medium;
the cling assembly comprises a pushing frame and an arc limiting plate, the top of the sliding supporting plate is connected with the pushing frame, and the second supporting frame is connected with the arc limiting plate.
2. The enzyme engineering-based strain culture device convenient for coating according to claim 1, wherein the strain culture device is characterized in that: the anti-sputtering device comprises a first support frame, and is characterized by further comprising an anti-sputtering assembly, wherein the anti-sputtering assembly is arranged on the first support frame and comprises a third support frame, a pushing plate, a fourth spring, a support rod, an arc-shaped slotted support plate, a limiting sliding frame, a sliding support frame, a fifth spring, a one-way ingress pipe, an arc-shaped extrusion plate, a sixth spring and a reset frame, wherein the top of the support cover plate is connected with the third support frame, the pushing plate is connected with the sliding on the third support frame, the fourth spring is connected between the pushing plate and the third support frame, the support rod is connected with the first support frame, one end of the support rod is connected with the arc-shaped slotted support plate, the limiting sliding frame is connected with the limiting sliding frame in a sliding manner, the fifth spring is connected between the sliding support frame and the limiting sliding support frame, the arc-shaped extrusion plate is connected with the sliding support frame in a contact manner, the sixth spring is connected between the arc-shaped slotted support plate and the limiting sliding frame, and the reset frame is connected with the support rod.
3. The enzyme engineering-based strain culture device convenient for coating according to claim 2, wherein the device is characterized in that: the device comprises a sputtering prevention assembly, and is characterized by further comprising an extrusion assembly, wherein the extrusion assembly is arranged on the sputtering prevention assembly and comprises a fourth support frame, a push plate frame, a seventh spring, a fifth support frame, a liquid storage frame, a threaded cover, a unidirectional guide pipe and a wedge-shaped extrusion block, the fourth support frame is connected to the top of the sliding support frame in a sliding manner, the push plate frame is connected to the sliding support frame in a sliding manner, the seventh spring is connected between the push plate frame and the fourth support frame, the fifth support frame is connected to the top of the limiting sliding frame, the liquid storage frame is connected to the top of the liquid storage frame through threads, the unidirectional guide pipe is communicated with the bottom of the liquid storage frame, the unidirectional guide pipe is communicated with the unidirectional guide pipe, and the wedge-shaped extrusion block is symmetrically connected to one side of the arc-shaped extrusion plate.
4. A strain culture device for facilitating coating based on enzyme engineering according to claim 3, characterized in that: the sealing assembly is arranged on the supporting cover plate and comprises a sliding perforated frame, an eighth spring, a sliding partition plate frame and a guide groove frame, the sliding perforated frame is connected to the top of the supporting cover plate in a sliding mode, the eighth spring is connected between the sliding perforated frame and the supporting cover plate, the sliding partition plate frame is connected to the bottom of the sliding perforated frame in a sliding mode, the guide groove frame is connected to the bottom of the supporting cover plate in a connecting mode, and the guide groove frame is in limiting fit with the sliding partition plate frame.
5. The enzyme engineering-based strain culture device convenient for coating according to claim 4, wherein the strain culture device is characterized in that: the support cover plate is connected with the first support frame, the arc inclined plate is fixedly connected with the first support frame, and the arc inclined plate is in contact with the first limit column.
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Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03133375A (en) * | 1989-10-18 | 1991-06-06 | Takeda Chem Ind Ltd | Applicator of inoculation solution to surface of agar medium |
| JPH104952A (en) * | 1996-06-26 | 1998-01-13 | Kayagaki Irika Kogyo Kk | Apparatus for coating culture medium and coating of culture medium |
| JPH10309199A (en) * | 1997-05-13 | 1998-11-24 | Yoshikawa Kogyo Co Ltd | Automatic coating of specimen and device |
| JPH11276155A (en) * | 1998-03-27 | 1999-10-12 | Yoshikawa Kogyo Co Ltd | Application apparatus for specimen and method therefor |
| JP2003047498A (en) * | 2001-08-07 | 2003-02-18 | Shibazaki Seisakusho:Kk | Automatic coating method for specimen |
| CN104152343A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Spin coating device |
| CN206308354U (en) * | 2016-12-23 | 2017-07-07 | 中检集团中原农食产品检测（河南）有限公司 | One kind is used for laboratory fluids bacterium solution uniform coating device |
| CN107034140A (en) * | 2017-05-25 | 2017-08-11 | 句容亿格纳米材料厂 | A kind of nanogel slant medium and preparation method thereof |
| CN207685289U (en) * | 2017-12-19 | 2018-08-03 | 河南科技学院 | A kind of microorganism apparatus for coating reducing contamination rate |
| CN208008803U (en) * | 2017-12-22 | 2018-10-26 | 杨宗雷 | A kind of bacterial sampling rolling cloth of coating-type apparatus for coating |
| CN108841571A (en) * | 2018-07-11 | 2018-11-20 | 张明明 | A kind of microculture vessel cleaning device |
| CN109182103A (en) * | 2018-11-02 | 2019-01-11 | 重庆英特力科技有限公司 | Automatic microbe apparatus for coating |
| CN109251850A (en) * | 2018-11-13 | 2019-01-22 | 无锡市疾病预防控制中心 | A kind of automatic L stick of hand-held |
| JP2019165676A (en) * | 2018-03-23 | 2019-10-03 | 国立大学法人東北大学 | Plant disease control agent and method of controlling plant disease |
| CN210176861U (en) * | 2019-05-13 | 2020-03-24 | 中检集团中原农食产品检测（河南）有限公司 | Automatic quantitative coating device for laboratory bacteria liquid |
| CN212894719U (en) * | 2020-06-12 | 2021-04-06 | 云南农业大学 | Coating device capable of uniformly coating bacterial liquid |
| CN213357547U (en) * | 2020-10-10 | 2021-06-04 | 天水市疾病预防控制中心 | Culture dish coating auxiliary device |
| CN213895825U (en) * | 2020-11-09 | 2021-08-06 | 河北维尔利动物药业集团有限公司 | Storage device for microbial bacteria activation |
| CN113621478A (en) * | 2021-08-02 | 2021-11-09 | 梁宗权 | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture |
| CN113632683A (en) * | 2021-08-10 | 2021-11-12 | 雷丁懿 | Liquid culture method for bacteria |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1412474A4 (en) * | 2001-08-03 | 2009-04-08 | Corning Inc | Removable splash guards for culture plates |
| ZA200602094B (en) * | 2006-01-16 | 2007-11-28 | Reliance Life Sciences Pvt Ltd | Device for culturing and transporting cells |
-
2021
- 2021-12-15 CN CN202111532768.XA patent/CN114058488B/en active Active
Patent Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03133375A (en) * | 1989-10-18 | 1991-06-06 | Takeda Chem Ind Ltd | Applicator of inoculation solution to surface of agar medium |
| JPH104952A (en) * | 1996-06-26 | 1998-01-13 | Kayagaki Irika Kogyo Kk | Apparatus for coating culture medium and coating of culture medium |
| JPH10309199A (en) * | 1997-05-13 | 1998-11-24 | Yoshikawa Kogyo Co Ltd | Automatic coating of specimen and device |
| JPH11276155A (en) * | 1998-03-27 | 1999-10-12 | Yoshikawa Kogyo Co Ltd | Application apparatus for specimen and method therefor |
| JP2003047498A (en) * | 2001-08-07 | 2003-02-18 | Shibazaki Seisakusho:Kk | Automatic coating method for specimen |
| CN104152343A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Spin coating device |
| CN206308354U (en) * | 2016-12-23 | 2017-07-07 | 中检集团中原农食产品检测（河南）有限公司 | One kind is used for laboratory fluids bacterium solution uniform coating device |
| CN107034140A (en) * | 2017-05-25 | 2017-08-11 | 句容亿格纳米材料厂 | A kind of nanogel slant medium and preparation method thereof |
| CN207685289U (en) * | 2017-12-19 | 2018-08-03 | 河南科技学院 | A kind of microorganism apparatus for coating reducing contamination rate |
| CN208008803U (en) * | 2017-12-22 | 2018-10-26 | 杨宗雷 | A kind of bacterial sampling rolling cloth of coating-type apparatus for coating |
| JP2019165676A (en) * | 2018-03-23 | 2019-10-03 | 国立大学法人東北大学 | Plant disease control agent and method of controlling plant disease |
| CN108841571A (en) * | 2018-07-11 | 2018-11-20 | 张明明 | A kind of microculture vessel cleaning device |
| CN109182103A (en) * | 2018-11-02 | 2019-01-11 | 重庆英特力科技有限公司 | Automatic microbe apparatus for coating |
| CN109251850A (en) * | 2018-11-13 | 2019-01-22 | 无锡市疾病预防控制中心 | A kind of automatic L stick of hand-held |
| CN210176861U (en) * | 2019-05-13 | 2020-03-24 | 中检集团中原农食产品检测（河南）有限公司 | Automatic quantitative coating device for laboratory bacteria liquid |
| CN212894719U (en) * | 2020-06-12 | 2021-04-06 | 云南农业大学 | Coating device capable of uniformly coating bacterial liquid |
| CN213357547U (en) * | 2020-10-10 | 2021-06-04 | 天水市疾病预防控制中心 | Culture dish coating auxiliary device |
| CN213895825U (en) * | 2020-11-09 | 2021-08-06 | 河北维尔利动物药业集团有限公司 | Storage device for microbial bacteria activation |
| CN113621478A (en) * | 2021-08-02 | 2021-11-09 | 梁宗权 | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture |
| CN113632683A (en) * | 2021-08-10 | 2021-11-12 | 雷丁懿 | Liquid culture method for bacteria |
Non-Patent Citations (2)
| Title |
|---|
| 固体培养基螺旋接种和涂布接种方法比较研究;卢勉飞;蔡芷荷;叶青华;李艳嫦;吴清平;杨绪伟;;中国卫生检验杂志(第04期);第512-515页 * |
| 基于高通量测序和培养组学的血小板中细菌检测方法的建立;王少礼等;《中国输血杂志》(第4期);第321-327页 * |
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