CN114058488A - Strain culture device convenient to coat based on enzyme engineering - Google Patents
Strain culture device convenient to coat based on enzyme engineering Download PDFInfo
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- CN114058488A CN114058488A CN202111532768.XA CN202111532768A CN114058488A CN 114058488 A CN114058488 A CN 114058488A CN 202111532768 A CN202111532768 A CN 202111532768A CN 114058488 A CN114058488 A CN 114058488A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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Abstract
The invention relates to the field of enzyme engineering, in particular to a strain culture device convenient for coating based on enzyme engineering, which comprises a support base, a first support frame, a second support frame, a first rotating frame and the like; the supporting base is connected with a first supporting frame through a bolt, the top of the first supporting frame is connected with a second supporting frame, and the first supporting frame is rotatably connected with a first rotating frame. The second rotating frame can be tightly attached to the surface of the agar culture medium and rotates, so that the bacterium liquid on the surface of the agar culture medium can be uniformly coated, the strain can be conveniently cultured, the agar culture medium can be guaranteed not to be damaged, and the effects of automatically and efficiently uniformly coating the bacterium liquid on the surface of the agar culture medium and guaranteeing the integrity of the agar culture medium are achieved.
Description
Technical Field
The invention relates to the field of enzyme engineering, in particular to a strain culture device convenient for coating based on enzyme engineering.
Background
In the method of artificially growing and breeding a large amount of pure strains under laboratory conditions, called strain culture, in studies on mildew resistance and bacteria resistance, it is generally necessary to separately culture microorganisms causing mildew decay and separate microorganisms of different species from a heterogeneous population of microorganisms to obtain pure strains. The methods for obtaining pure species are many, but the basic principle is similar, namely, the separated sample is diluted to a certain degree, cells of the microorganism exist in a dispersed state as much as possible, then the cells grow into single colonies, and then the single colonies are transferred to a proper culture medium, and the pure species microorganism is generally obtained.
In the process of strain culture, need with the fungus liquid coating on the culture medium, prior art is manual on pouring into the fungus liquid into the culture medium, evenly coat the fungus liquid with the coating rod rapidly immediately, promptly hold the culture dish with the left hand, and make the dish lid open a seam, the coating rod is held at the culture medium surface to the right hand and is removed, in order to reach the effect of coating the fungus liquid, but the easy error of prior art people's manual operation, lead to the culture medium to be pushed open, be unfavorable for the cultivation of fungus, and when the fungus liquid was poured into to the people hand, need open the dish lid, lead to the inside bacterium that gets into easily of culture dish, influence the cultivation of fungus.
Disclosure of Invention
In view of the above, there is a need for a bacterial culture apparatus based on enzyme engineering, which can automatically and efficiently coat the bacterial liquid on the surface of the agar culture medium uniformly and ensure the integrity of the agar culture medium, and can maintain the culture dish in a closed culture environment, so as to solve the problems that the prior art provided by the background art is easy to push the culture medium due to errors in manual operation, and bacteria are easy to enter the interior of the culture dish.
The technical implementation scheme of the invention is as follows: the utility model provides a bacterial culture apparatus based on enzyme engineering coating of being convenient for, including supporting base, first support frame, second support frame, first revolving rack, place frame, spacing, culture dish, support apron, first spring, coating unit and hug closely the subassembly:
the first support frame is connected to the support base through bolts and is of a circular structure;
the top of the first support frame is connected with a second support frame;
the first support frame is connected with a first rotating frame in a rotating mode;
the first rotating frame is fixedly connected with the placing frame which adopts a circular structure;
the top of the placing frame is connected with a limiting frame;
a culture dish is placed on the placing frame and used for culturing strains;
the limiting frame is connected with the support cover plate in a sliding mode, the support cover plate is rectangular, and the support cover plate is used for covering the culture dish;
the first spring is connected between the supporting cover plate and the limiting frame;
the coating assembly is arranged on the supporting cover plate and used for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium;
and the coating component is provided with a clinging component which is used for clinging the coating component to the surface of the agar culture medium.
More preferably, the coating subassembly is including the arc rack, first spacing post, the slip backup pad, the rotation fluting board, the second spring, the second rotating turret, the third spring, rotation support ring and straight-teeth gear, the hookup has the arc rack on the second support frame, support apron top symmetry hookup has first spacing post, common slidingtype connection has the slip backup pad between two first spacing posts, the inside rotary type of slip backup pad is connected with the rotation fluting board, the symmetric connection has the second spring between slip backup pad and the support apron, the slidingtype is connected with the second rotating turret on the rotation fluting board, be connected with the third spring between second rotating turret and the rotation fluting board, the last rotation type of support apron is connected with the rotation support ring, rotation support ring and second rotating turret slidingtype cooperation, the hookup of rotation support ring top has the straight-teeth gear.
More preferably, the lower part of the second rotating frame adopts a straight-line structure, and the straight-line structure is used for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium.
More preferably, the clinging component comprises a pushing frame and an arc limiting plate, the top of the sliding support plate is connected with the pushing frame, and the second support frame is connected with the arc limiting plate.
More preferably, the device also comprises an anti-sputtering component, the anti-sputtering component is arranged on the first support frame and comprises a third support frame, a pushing plate, a fourth spring, a support rod, an arc-shaped slotted support plate, a limit sliding frame, a sliding support frame, a fifth spring, a one-way lead-in pipe, an arc-shaped extrusion plate, a sixth spring and a reset frame, the top of the support cover plate is connected with the third support frame, the third support frame is connected with the pushing plate in a sliding way, the fourth spring is connected between the pushing plate and the third support frame, the support rod is connected on the first support frame, one end of the support rod is connected with the arc-shaped slotted support plate, the arc-shaped slotted support plate is connected with the limit sliding frame in a sliding way, the limit sliding frame is connected with the sliding support frame in a sliding way, the fifth spring is connected between the sliding support frame and the limit sliding frame, the bottom of the sliding support frame is connected with the one-way lead-in pipe, an arc-shaped extrusion plate is connected to the support rod, the arc-shaped extrusion plate is in contact with the sliding support frame, a sixth spring is connected between the arc-shaped slotting support plate and the limiting sliding frame, and a reset frame is connected to the support rod.
More preferably, still including crowded subassembly, crowded subassembly is located on the anti-sputtering subassembly, crowded subassembly is including the fourth support frame, push away the grillage, the seventh spring, the fifth support frame, the stock solution frame, the screw cap, one-way honeycomb duct and wedge extrusion piece, the hookup of slip support frame top has the fourth support frame, the inside slidingtype of slip support frame is connected with push away the grillage, be connected with the seventh spring between push away the grillage and the fourth support frame, the hookup has the fifth support frame on the spacing carriage, the hookup of fifth support frame top has the stock solution frame, there is the screw cap stock solution frame top through threaded connection, stock solution frame outer bottom intercommunication has one-way honeycomb duct, one-way honeycomb duct and one-way leading-in pipe switch-on, arc extrusion plate one side symmetry hookup has the wedge extrusion piece.
More preferably, still including airtight subassembly, airtight subassembly is located and is supported on the apron, airtight subassembly is including sliding slotted frame, eighth spring, slip baffle frame and guide way frame, supports apron top sliding connection and has sliding slotted frame, is connected with the eighth spring between slip slotted frame and the support apron, and sliding slotted frame bottom sliding connection has sliding baffle frame, supports the apron bottom hookup and has guide way frame, guide way frame and the spacing cooperation of sliding baffle frame.
More preferred is, still including the subassembly that opens and shuts, the subassembly that opens and shuts is located on supporting the apron, and the subassembly that opens and shuts is including spacing post of second and pitch arc swash plate, and the hookup has the spacing post of second on the supporting apron, fixedly connected with pitch arc swash plate on the first support frame, pitch arc swash plate and the spacing post contact of second.
Compared with the prior art, the invention has the following advantages:
the second rotating frame can be tightly attached to the surface of the agar culture medium and rotates, so that the bacterium liquid on the surface of the agar culture medium can be uniformly coated, the strain can be conveniently cultured, the agar culture medium can be guaranteed not to be damaged, and the effects of automatically and efficiently uniformly coating the bacterium liquid on the surface of the agar culture medium and guaranteeing the integrity of the agar culture medium are achieved.
The one-way introducing pipe and the device on the one-way introducing pipe can move downwards and are inserted into the supporting cover plate, so that the one-way introducing pipe is close to the surface of the agar culture medium, the bacteria liquid can be conveniently and completely injected to the surface of the agar culture medium, and the aim of effectively preventing the bacteria liquid from being injected is fulfilled.
The one-way introducing pipe can extract the bacteria liquid and inject the bacteria liquid into the surface of the agar culture medium, thereby providing convenience for culturing strains and achieving the effect of automatically injecting the bacteria liquid into the surface of the agar culture medium.
At the in-process to agar medium surface injection fungus liquid, the sliding partition frame can be opened, and after the injection finishes, the sliding partition frame then can close at once, is convenient for keep the inclosed cultivateing environment of culture dish, prevents that the inside bacterial of culture dish from receiving external environment's pollution, is favorable to the normal growth of the inside bacterial of culture dish to breed.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention.
Fig. 2 is a schematic perspective view of a second embodiment of the present invention.
Fig. 3 is a perspective view of the cling assembly of the present invention.
Fig. 4 is a partially disassembled perspective view of a coating module of the present invention.
FIG. 5 is a schematic view of a first partial body structure according to the present invention.
FIG. 6 is a schematic view of a first partially separated body structure of the sputtering prevention assembly of the present invention.
FIG. 7 is a schematic view of a second partial body structure according to the present invention.
FIG. 8 is a schematic view of a second partial body structure of the sputtering prevention assembly of the present invention.
Fig. 9 is a perspective view of a third embodiment of the present invention.
FIG. 10 is a perspective view of a third portion of the sputtering prevention assembly of the present invention.
FIG. 11 is a schematic perspective view of a portion of the squeeze-in assembly of the present invention.
Fig. 12 is a perspective view of a fourth embodiment of the present invention.
Fig. 13 is a schematic perspective view of a portion of a closure assembly according to the present invention.
Fig. 14 is a perspective view of the closure assembly of the present invention.
Fig. 15 is a schematic perspective view of the opening and closing assembly of the present invention.
The parts are labeled as follows: 1. the device comprises a supporting base, 22, a first supporting frame, 23, a second supporting frame, 24, a first rotating frame, 25, a placing frame, 26, a limiting frame, 261, a culture dish, 27, a supporting cover plate, 28, a first spring, 3, a coating assembly, 31, an arc-shaped rack, 32, a first limiting column, 33, a sliding supporting plate, 331, a rotary grooving plate, 34, a second spring, 35, a second rotating frame, 36, a third spring, 37, a rotary supporting ring, 38, a straight gear, 4, a clinging assembly, 41, a pushing frame, 42, an arc-shaped limiting plate, 5, an anti-sputtering assembly, 51, a third supporting frame, 52, a pushing plate, 53, a fourth spring, 54, a supporting rod, 55, an arc-shaped grooving supporting plate, 56, a limiting sliding frame, 57, a sliding supporting frame, 571, a fifth spring, 58, a one-way leading-in pipe, 59, an arc-shaped extrusion plate, 510, a sixth spring, 511, a resetting frame, 6, a one-way leading-in pipe, a limiting frame, a second rotating frame, a third rotating frame, a fourth rotating frame, The extrusion subassembly, 61, fourth support frame, 62, push pedal frame, 63, seventh spring, 64, fifth support frame, 65, stock solution frame, 66, screw cap, 67, one-way honeycomb duct, 68, wedge extrusion piece, 7, airtight subassembly, 71, slip trompil frame, 72, eighth spring, 73, slip baffle frame, 74, guide way frame, 8, subassembly that opens and shuts, 81, the spacing post of second, 82, pitch arc swash plate.
Detailed Description
The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
A strain culture device convenient for coating based on enzyme engineering is disclosed, as shown in figure 1-5, comprising a support base 1, a first support frame 22, a second support frame 23, a first rotating frame 24, a placing frame 25, a limiting frame 26, a culture dish 261, a support cover plate 27, a first spring 28, a coating component 3 and a clinging component 4, wherein the support base 1 is in a circular structure, the support base 1 is connected with the first support frame 22 through bolts, the top of the first support frame 22 is connected with the second support frame 23, the second support frame 23 is in an N-shaped structure, the first support frame 22 is rotatably connected with the first rotating frame 24, the placing frame 24 is fixedly connected with the placing frame 25, the placing frame 25 is in a circular structure, the top of the placing frame 25 is connected with the limiting frame 26, the placing frame 25 is provided with the culture dish 261 for culturing strains, the support cover plate 27 is rotatably connected on the limiting frame 26, the supporting cover plate 27 is rectangular, a first spring 28 is connected between the supporting cover plate 27 and the limiting frame 26, the supporting cover plate 27 is provided with a coating component 3 which is used for uniformly coating the bacterium liquid dripped on the surface of the agar culture medium, and the coating component 3 is provided with a clinging component 4 which is used for enabling the coating component 3 to cling to the surface of the agar culture medium.
The coating component 3 comprises an arc-shaped rack 31, a first limit column 32, a sliding support plate 33, a rotary slotted plate 331, a second spring 34, a second rotary frame 35, a third spring 36, a rotary support ring 37 and a straight gear 38, the arc-shaped rack 31 is connected on the second support frame 23, the arc-shaped rack 31 adopts an arc-shaped structure, the first limit column 32 is symmetrically connected on the top of the support cover plate 27, the sliding support plate 33 is jointly and slidably connected between the two first limit columns 32, the rotary slotted plate 331 is rotatably connected in the sliding support plate 33, the second spring 34 is symmetrically connected between the sliding support plate 33 and the support cover plate 27, the second rotary frame 35 for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium is slidably connected on the rotary slotted plate 331, the bottom of the second rotary frame 35 is in a straight-line structure, the third spring 36 is connected between the second rotary frame 35 and the rotary slotted plate 331, the supporting cover plate 27 is rotatably connected with a rotating supporting ring 37, the rotating supporting ring 37 is slidably matched with the second rotating frame 35, and the top of the rotating supporting ring 37 is connected with a spur gear 38.
The clinging component 4 comprises a pushing frame 41 and an arc limiting plate 42, the top of the sliding support plate 33 is connected with the pushing frame 41, the pushing frame 41 adopts an N-shaped structure, the second support frame 23 is connected with the arc limiting plate 42, and the arc limiting plate 42 is arc-shaped.
When the culture dish is used, the supporting cover plate 27 and the upper device thereof are pushed manually to move upwards, the first spring 28 is compressed along with the supporting cover plate, the sterilized agar culture medium is poured into the culture dish 261 manually, the supporting cover plate 27 is loosened, the first spring 28 resets along with the supporting cover plate and drives the supporting cover plate 27 and the upper device thereof to reset, so that the supporting cover plate 27 covers the culture dish 261 to prevent external bacteria from influencing the agar culture medium in the culture dish 261, bacteria liquid is dripped onto the surface of the agar culture medium on the culture dish 261 through small holes in the supporting cover plate 27 manually, the placing frame 25 is rotated clockwise manually to rotate the placing frame 25 and the upper device thereof, the pushing frame 41 is contacted with the arc limiting plate 42 in the process, the arc limiting plate 42 pushes the pushing frame 41 and the upper device thereof to move downwards, the second spring 34 is compressed along with the second spring, when the second rotating frame 35 is contacted with the agar culture medium in the culture dish 261, the third spring 36 is stretched to enable the second rotating frame 35 to be tightly attached to the agar culture medium in the culture dish 261, then the straight gear 38 is in contact with the arc-shaped rack 31, under the action of the arc-shaped rack 31, the straight gear 38 and the device on the straight gear 38 rotate and enable the second rotating frame 35 to evenly coat the bacterial liquid dropping on the surface of the agar culture medium, then the pushing frame 41 is separated from the arc-shaped limiting plate 42, the second spring 34 resets and drives the pushing frame 41 and the device on the pushing frame to reset, the third spring 36 resets and drives the second rotating frame 35 to reset, and the device can be used for coating the bacterial liquid again by repeating the operations.
Example 2
On the basis of embodiment 1, as shown in fig. 5 to 10, the injection molding device further includes a sputtering prevention assembly 5, the sputtering prevention assembly 5 for preventing the bacteria liquid from splashing during the injection process is disposed on the first support frame 22, the sputtering prevention assembly 5 includes a third support frame 51, a pushing plate 52, a fourth spring 53, a support rod 54, an arc-shaped slotted support plate 55, a limit carriage 56, a sliding support frame 57, a fifth spring 571, a one-way introducing pipe 58, an arc-shaped extrusion plate 59, a sixth spring 510 and a return frame 511, the third support frame 51 is connected to the top of the support cover plate 27, the pushing plate 52 is slidably connected to the third support frame 51, the fourth spring 53 is connected between the pushing plate 52 and the third support frame 51, the support rod 54 is connected to the first support frame 22, an arc-shaped support plate slot 55 is connected to one end of the support rod 54, an arc-shaped slotted support plate 55 is provided with an arc-shaped sliding slot, the arc-shaped slotted support plate 55 is slidably connected to the limit carriage 56, the limiting sliding frame 56 is connected with a sliding support frame 57 in a sliding mode, the inside of the sliding support frame 57 is of a hollow structure, a fifth spring 571 is connected between the sliding support frame 57 and the limiting sliding frame 56, the bottom of the sliding support frame 57 is connected with a one-way leading-in pipe 58 for injecting bacteria liquid, the inside of the one-way leading-in pipe 58 is of a hollow structure, the support rod 54 is connected with an arc extrusion plate 59, one side of the arc extrusion plate 59 is of an inclined plane structure, the arc extrusion plate 59 is in contact with the sliding support frame 57, a sixth spring 510 is connected between the arc slotting support plate 55 and the limiting sliding frame 56, the support rod 54 is connected with a reset frame 511, and the reset frame 511 is of an L shape.
During the rotation of the placing frame 25 and the device thereon, the pushing plate 52 will contact with the limit sliding frame 56, the pushing plate 52 will push the limit sliding frame 56 and the device thereon to move together, the sixth spring 510 will be compressed, during the process, the arc-shaped extrusion plate 59 will extrude the sliding support frame 57 and the device thereon to move downwards and make the one-way introducing pipe 58 insert into the support cover plate 27, the fifth spring 571 will be compressed therewith, then the bacteria liquid will be injected into the limit sliding frame 56 manually, the bacteria liquid can be injected into the surface of the agar culture medium through the one-way introducing pipe 58 to prevent the bacteria liquid from splashing during the injection, then the pushing plate 52 will contact with the reset frame 511, the reset frame 511 will extrude the pushing plate 52 to move downwards and make it separate from the limit sliding frame 56, the fourth spring 53 will be compressed therewith, the limit sliding frame 56 and the device thereon will be reset reversely, so that the bacterial liquid can be fully and completely injected to the surface of the agar culture medium.
Example 3
On the basis of the embodiment 2, as shown in fig. 9-12, the device further comprises an extruding assembly 6, the extruding assembly 6 for extruding the bacteria liquid inside the one-way introducing pipe 58 to the surface of the agar medium is arranged on the sputtering prevention assembly 5, the extruding assembly 6 comprises a fourth support frame 61, a push plate frame 62, a seventh spring 63, a fifth support frame 64, a liquid storage frame 65, a threaded cover 66, a one-way flow guiding pipe 67 and a wedge-shaped extruding block 68, the top of the sliding support frame 57 is connected with the fourth support frame 61, the inside of the sliding support frame 57 is connected with the push plate frame 62 in a sliding manner, the lower part of the push plate frame 62 is disc-shaped, the seventh spring 63 is connected between the push plate frame 62 and the fourth support frame 61, the fifth support frame 64 is connected to the limiting sliding frame 56, the top of the fifth support frame 64 is connected with the liquid storage frame 65, the top of the liquid storage frame 65 is connected with the threaded cover 66 for covering the liquid storage frame 65 through threads, the outer bottom of the liquid storage frame 65 is communicated with a one-way guide pipe 67 for conveying the bacterial liquid, the one-way guide pipe 67 is communicated with the one-way guide pipe 58, and one side of the arc-shaped extrusion plate 59 is symmetrically connected with wedge-shaped extrusion blocks 68.
The liquid storage frame 65 is internally provided with a certain amount of bacteria liquid, in the process of moving the limiting sliding frame 56 and the device on the limiting sliding frame, the push plate frame 62 is contacted with one wedge-shaped extrusion block 68, one wedge-shaped extrusion block 68 extrudes the push plate frame 62 to move downwards and extrude the air in the sliding support frame 57, the seventh spring 63 is stretched along with the downward movement, then the push plate frame 62 is separated from one wedge-shaped extrusion block 68, the seventh spring 63 is reset along with the downward movement and drives the push plate frame 62 to reset, the bacteria liquid in the liquid storage frame 65 is pumped into the one-way introducing pipe 58 through the one-way guide pipe 67, then the push plate frame 62 is contacted with the other wedge-shaped extrusion block 68, the other wedge-shaped extrusion block 68 extrudes the push plate frame 62 to move downwards and extrude the bacteria liquid in the one-way introducing pipe 58 to the surface of the agar culture medium, and then the push plate frame 62 is separated from the other wedge-shaped extrusion block 68, the push plate frame 62 is reset, the one-way guide pipe 67 and the one-way guide pipe 58 only allow the bacteria liquid to pass through in one way in the process, the bacteria liquid is prevented from flowing back, after the bacteria liquid is injected once, a person needs to manually open the threaded cover 66 and add a certain amount of bacteria liquid into the liquid storage frame 65, then manually close the threaded cover 66, and the operations are repeated to automatically inject the bacteria liquid into the surface of the agar culture medium again.
Example 4
On the basis of embodiment 3, as shown in fig. 13 to 14, the culture dish 261 further includes a sealing assembly 7, the sealing assembly 7 for ensuring a sealed environment inside the culture dish 261 is disposed on the supporting cover plate 27, the sealing assembly 7 includes a sliding opening frame 71, an eighth spring 72, a sliding partition frame 73 and a guiding slot frame 74, the sliding opening frame 71 is slidably connected to the top of the supporting cover plate 27, the sliding opening frame 71 is Z-shaped, the eighth spring 72 is connected between the sliding opening frame 71 and the supporting cover plate 27, the sliding partition frame 73 is slidably connected to the bottom of the sliding opening frame 71, the guiding slot frame 74 is connected to the bottom of the supporting cover plate 27, an inclined sliding slot is formed in the guiding slot frame 74, and the guiding slot frame 74 is in limit fit with the sliding partition frame 73.
During the downward movement of the one-way introducing pipe 58 and the devices thereon, the one-way introducing pipe 58 extrudes the sliding perforated frame 71 and the devices thereon move downward, the eighth spring 72 is compressed, the sliding partition frame 73 moves along the sliding groove on the guide groove frame 74 under the action of the guide groove frame 74, so that the sliding partition frame 73 is opened, the bacteria liquid is conveniently injected, when the one-way introducing pipe 58 and the devices thereon reset, the eighth spring 72 resets and drives the sliding perforated frame 71 and the devices thereon to reset, and the sliding partition frame 73 closes accordingly, so as to ensure the sealed environment inside the culture dish 261.
Example 5
On embodiment 4's basis, as shown in fig. 15, still including the subassembly 8 that opens and shuts, the subassembly 8 that opens and shuts is located on the support apron 27, the subassembly 8 that opens and shuts is including spacing post 81 of second and pitch arc swash plate 82, the hookup has spacing post 81 of second on the support apron 27, the spacing post 81 of second is cylindricly, fixedly connected with pitch arc swash plate 82 on the first support frame 22, pitch arc swash plate 82 adopts two inclined plane structures, pitch arc swash plate 82 contacts with spacing post 81 of second.
In the process of placing the frame 25 and rotating the upper device thereof, the second limit column 81 can be separated from the arc sloping plate 82, the compressed first spring 28 can be restored and drive the support cover plate 27 and the upper device thereof to move downwards, so that the support cover plate 27 is closed, the second limit column 81 can be contacted with the arc sloping plate 82 again, the arc sloping plate 82 can extrude the second limit column 81 and the upper device thereof to reset upwards, the first spring 28 can be compressed to reset along with the compression, the support cover plate 27 is opened, the support cover plate 27 is pushed to be opened manually instead of a human, and the agar culture medium can be poured into the culture dish 261 conveniently.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Claims (8)
1. A bacterial culture device convenient for coating based on enzyme engineering is characterized in that: including supporting base, first support frame, second support frame, first revolving rack, placing frame, spacing, culture dish, support apron, first spring, coating unit and hug closely the subassembly:
the first support frame is connected to the support base through bolts and is of a circular structure;
the top of the first support frame is connected with a second support frame;
the first support frame is connected with a first rotating frame in a rotating mode;
the first rotating frame is fixedly connected with the placing frame which adopts a circular structure;
the top of the placing frame is connected with a limiting frame;
a culture dish is placed on the placing frame and used for culturing strains;
the limiting frame is connected with the support cover plate in a sliding mode, the support cover plate is rectangular, and the support cover plate is used for covering the culture dish;
the first spring is connected between the supporting cover plate and the limiting frame;
the coating assembly is arranged on the supporting cover plate and used for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium;
and the coating component is provided with a clinging component which is used for clinging the coating component to the surface of the agar culture medium.
2. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 1, wherein: coating subassembly is including the arc rack, first spacing post, the slip backup pad, the grooving board rotates, the second spring, the second rotating turret, the third spring, support ring and straight-teeth gear rotate, the hookup has the arc rack on the second support frame, it has first spacing post to support the symmetrical hookup in apron top, common slidingtype is connected with the slip backup pad between two first spacing posts, the inside rotary type of slip backup pad is connected with the grooving board of rotation, the symmetric connection has the second spring between slip backup pad and the support apron, it has the second rotating turret to rotate the sliding connection on the grooving board, be connected with the third spring between second rotating turret and the rotation grooving board, the last rotary type of support apron is connected with the rotation support ring, rotation support ring and the slidingtype cooperation of second rotating turret, the hookup of rotation support ring top has straight-teeth gear.
3. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 2, wherein: the lower part of the second rotating frame adopts a straight-line structure and is used for uniformly coating the bacterial liquid dropped on the surface of the agar culture medium.
4. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 2, wherein: the clinging component comprises a pushing frame and an arc limiting plate, the top of the sliding supporting plate is connected with the pushing frame, and the second supporting frame is connected with the arc limiting plate.
5. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 4, wherein: the anti-sputtering device also comprises an anti-sputtering component, the anti-sputtering component is arranged on the first support frame and comprises a third support frame, a pushing plate, a fourth spring, a support rod, an arc-shaped slotted support plate, a limiting sliding frame, a sliding support frame, a fifth spring, a one-way lead-in pipe, an arc-shaped extrusion plate, a sixth spring and a reset frame, the top of the support cover plate is connected with the third support frame, the pushing plate is connected on the third support frame in a sliding way, the fourth spring is connected between the pushing plate and the third support frame, the support rod is connected on the first support frame in a connecting way, the arc-shaped slotted support plate is connected at one end of the support rod, the limiting sliding frame is connected on the arc-shaped slotted support plate in a sliding way, the sliding support frame is connected on the limiting sliding frame in a sliding way, the fifth spring is connected between the sliding support frame and the limiting sliding frame, the one-way lead-in pipe is connected at the bottom of the sliding support frame, the arc-shaped extrusion plate is connected on the support rod, the arc-shaped extrusion plate is contacted with the sliding support frame, a sixth spring is connected between the arc-shaped slotting support plate and the limiting sliding frame, and the support rod is connected with a reset frame.
6. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 5, wherein: still including crowded subassembly, crowded subassembly is located on the anti-sputtering subassembly, crowded subassembly is including the fourth support frame, push away the grillage, the seventh spring, the fifth support frame, the stock solution frame, the screw cap, one-way honeycomb duct and wedge extrusion piece, the hookup of slip support frame top has the fourth support frame, the inside slidingtype of slip support frame is connected with push away the grillage, be connected with the seventh spring between push away the grillage and the fourth support frame, the hookup has the fifth support frame on the spacing carriage, the hookup of fifth support frame top has the stock solution frame, there is the screw cap stock solution frame top through threaded connection, stock solution frame outer bottom intercommunication has one-way honeycomb duct, one-way honeycomb duct and one-way leading-in pipe switch-on, arc extrusion board one side symmetry hookup has the wedge extrusion piece.
7. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 6, wherein: still including airtight subassembly, airtight subassembly is located and is supported on the apron, airtight subassembly is including sliding trompil frame, eighth spring, slip baffle frame and guide way frame, supports apron top sliding connection and has sliding trompil frame, is connected with the eighth spring between sliding trompil frame and the support apron, and sliding trompil frame bottom sliding connection has the slip baffle frame, supports the connection of apron bottom and has the guide way frame, guide way frame and the spacing cooperation of slip baffle frame.
8. A seed culture apparatus for facilitating coating based on enzyme engineering according to claim 7, wherein: still including the subassembly that opens and shuts, the subassembly that opens and shuts is located on the support apron, and the subassembly that opens and shuts is including spacing post of second and pitch arc swash plate, and the hookup has the spacing post of second on the support apron, fixedly connected with pitch arc swash plate on the first support frame, pitch arc swash plate and the spacing post contact of second.
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