CN113632683A - Liquid culture method for bacteria - Google Patents
Liquid culture method for bacteria Download PDFInfo
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- CN113632683A CN113632683A CN202110914789.1A CN202110914789A CN113632683A CN 113632683 A CN113632683 A CN 113632683A CN 202110914789 A CN202110914789 A CN 202110914789A CN 113632683 A CN113632683 A CN 113632683A
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- liquid
- liquid guide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/68—Cultivation bottles
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a method for culturing fungus liquid, which comprises the steps of placing a plurality of culture dishes placed with different edible fungus culture liquids in corresponding placing grooves for culturing, when culture solution needs to be added or oxygen needs to be supplemented, the liquid guide pipe and the liquid guide box are opened, and the supplement is carried out through the second feed liquid independently equipped on the liquid guide box corresponding to the culture dish, when the same group of culture dishes are used for co-culturing the same edible fungi, the adjacent three liquid guide boxes are commonly provided with the first liquid inlet hopper, after the supplement is finished, the liquid guide pipes and the liquid guide boxes are closed, the invention can separate and protect the culture dishes of different edible fungi, when the culture solution or oxygen can be conveniently supplemented to different strains, the culture of various strains can be flexibly combined, when the strain culture device is used in strain culture experiments, the efficiency is improved, the operation is simple and convenient, the sealing effect is good, and the conditions of leakage, pollution and the like can be effectively prevented.
Description
Technical Field
The invention relates to the field of edible fungus culture, in particular to a liquid culture method for edible fungus strains.
Background
The edible fungi are commonly called mushrooms, more than 350 kinds of the known edible fungi in China exist, strains need to be cultured firstly in the artificial cultivation of the edible fungi, liquid strains have the obvious advantages of short production period, low production cost, less automatic pollution, short cultivation period and the like compared with solid strains, the liquid cultivation of the strains is adopted by more and more cultivators, the liquid cultivation of the strains usually needs proper temperature, culture solution and oxygen are supplemented according to the needs, the most common liquid cultivation method in the industry at present is carried out in a reaction kettle, the method has the advantages of being capable of cultivating a large number of strains at one time, being easy to seal, control temperature and humidity and the like, but the culture conditions of the strains required by different edible fungi are different, the method is only suitable for the cultivation of specific strains, is not suitable for the cultivation of multiple types and small batches, and only can be directly filled in a culture dish, the culture dishes of different edible fungi are respectively placed in different areas, and the culture solution needs to be added one by one when needing to be added, which is very inconvenient.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a liquid culture method which can be used for various edible fungi.
The technical scheme adopted by the invention for solving the technical problems is as follows: a liquid culture method of fungi is characterized in that a liquid culture device of edible fungi is used for culture, the liquid culture device of the edible fungi comprises an airtight space in which a plurality of culture dishes are placed, the culture dishes penetrate through the airtight space through corresponding liquid guide pipes to be connected with corresponding liquid guide boxes, the plurality of culture dishes are grouped, the liquid guide boxes of each group of culture dishes are communicated with a first liquid inlet hopper, the liquid guide box of each culture dish is also connected with an independent second liquid inlet hopper, and the liquid guide pipes are opened or closed through a push plate;
the culture method comprises the steps of placing a plurality of culture dishes placed with different edible fungus strain culture solutions in corresponding placing grooves for culture, opening a liquid guide pipe and a liquid guide box when the culture solutions are required to be added or oxygen is required to be supplemented, supplementing through a second liquid inlet hopper independently arranged on the liquid guide box corresponding to the culture dishes, using three adjacent liquid guide boxes to be jointly provided with first liquid inlet hoppers when the same group of culture dishes are used for jointly culturing the same edible fungi, and closing the liquid guide pipe and the liquid guide boxes after the supplementation is finished.
The liquid culture device for the edible fungi comprises a counterweight base, wherein a plurality of culture dishes are placed on the top of the counterweight base at equal intervals, a protective cover is arranged above the counterweight base, supporting rods are inserted into the four corners of the top of the protective cover in a penetrating and sliding mode at equal intervals, a supporting plate is fixedly connected to the top of the supporting rod, a plurality of liquid guide boxes are arranged on the top of the supporting plate at equal intervals corresponding to the positions of the culture dishes, a first liquid inlet hopper is fixedly communicated with the top of each liquid guide box, a fixing cover is fixedly connected to the bottom of the supporting plate, the supporting rods penetrate through the four corners of the fixing cover, first springs are fixedly connected to the four corners of the bottom of the fixing cover, the first springs are sleeved on the rod wall of the supporting rod, one end, away from the fixing cover, of each first spring is fixedly connected with the protective cover, one end, penetrating through the supporting plate, of the bottom of each liquid guide box is fixedly communicated with a first fixing pipe, the inner top of the first fixed pipe is fixedly connected with a first fixed ring, the bottom of the first fixed ring is fixedly connected with a second spring, the other end of the second spring is fixedly connected with a first liquid guide pipe, the first liquid guide pipe is inserted in the first fixed pipe in a sliding manner, a plurality of second fixed pipes are fixedly inserted in the inner bottom of the fixed cover at equal intervals corresponding to the position of the first fixed pipe, the other end of each second fixed pipe penetrates through the protective cover, the second fixed pipes are connected with the protective cover in a sliding manner, the pipe wall of the inner top of each second fixed pipe is fixedly connected with a second fixed ring, the bottom of each second fixed ring is fixedly connected with a third spring, the other end of each third spring is fixedly connected with a third fixed ring, the third fixed ring is connected in the second fixed pipe in a sliding manner, and the second liquid guide pipe is fixedly inserted in the third fixed ring, the utility model discloses a fixing cover, including fixing cover, first push pedal, first recess, first groove, second groove, first spring, second spring, first push pedal and second push pedal, the both ends of fixing cover and both sides are slided respectively and are pegged graft and have been had first push pedal of a plurality of and second push pedal, first recess has all been seted up to first push pedal and second push pedal slip grafting to the inside both ends of fixing cover, fixedly connected with fourth spring on the first recess inner wall, the other end of fourth spring and the inner wall fixed connection of fixing cover, a plurality of second recess has been seted up to the impartial interval in top of first push pedal and second push pedal, the second recess sets up under first catheter, just the diameter of second recess is greater than the diameter of first catheter, the inside fixedly connected with shrouding of second recess.
Specifically, the heating plate is embedded in the counterweight base, and the placing groove matched with the top of the counterweight base is formed in the position, corresponding to the culture dish, of the top of the counterweight base.
Specifically, the inside of protection casing separates through the baffle and is equipped with three cavity, just inside three cavity and the setting of a plurality of culture dish phase-match of protection casing.
Specifically, the bottom of protection casing has seted up the seal groove, the inside scarf joint of seal groove has the sealing washer.
Specifically, it has the feed liquor pipe to be fixed the intercommunication on the outer wall of drain case one side, the slope of feed liquor pipe sets up, just the fixed intercommunication of one end that the drain case was kept away from to the feed liquor pipe has the second to advance the liquid fill.
Specifically, a tube plug is inserted into the inner top of the first catheter, a fixing plate is fixedly connected to the top of the tube plug, and the fixing plate is fixedly connected with the inner tube wall of the first fixing tube.
Specifically, the two ends of the first push plate and the second push plate are fixedly connected with the poking blocks, and the cross sectional area of each poking block is larger than the cross sectional areas of the two ends of the first push plate and the second push plate.
The invention has the beneficial effects that:
1. the culture dishes of different edible fungi can be separated and protected, so that culture solution or oxygen can be conveniently supplemented to different strains, the culture of various strains can be flexibly combined, and the efficiency is improved when the strain culture experiment is used;
2. simple operation, good sealing effect and can effectively prevent leakage, pollution and the like.
Drawings
FIG. 1 is a schematic view showing the construction of a liquid culture apparatus according to the present invention;
FIG. 2 is a plan view of a liquid culture apparatus of the present invention;
FIG. 3 is a schematic view showing the structure of a push plate in the liquid culture apparatus according to the present invention;
FIG. 4 is a schematic view showing the structure of a protective cover in the liquid culture apparatus of the present invention;
FIG. 5 is an enlarged view of a portion A in FIG. 1 of the liquid culture apparatus according to the present invention;
FIG. 6 is an enlarged view of a portion B in FIG. 1 of the liquid culture apparatus of the present invention.
In the figure: 1. a counterweight base; 2. heating plates; 3. a placement groove; 4. a culture dish; 5. a protective cover; 6. a partition plate; 7. a sealing groove; 8. a seal ring; 9. a support bar; 10. a support plate; 11. a liquid guide box; 12. a first liquid inlet hopper; 13. a liquid inlet pipe; 14. a second liquid inlet hopper; 15. a fixed cover; 16. a first spring; 17. a first stationary tube; 18. a first retaining ring; 19. a second spring; 20. a first catheter; 21. a second stationary tube; 22. a second retaining ring; 23. a third spring; 24. a third fixing ring; 25. a second catheter; 26. a first push plate; 27. a second push plate; 28. a shifting block; 29. a first groove; 30. a fourth spring; 31. a second groove; 32. closing the plate; 33. a pipe plug; 34. and (7) fixing the plate.
Detailed Description
The liquid culture device for the edible fungi used in the fungus liquid culture method comprises a counterweight base 1, 9 culture dishes 4 are placed on the top of the counterweight base 1 at equal intervals, a heating plate 2 is embedded in the counterweight base 1, a placing groove 3 matched with the counterweight base 1 is formed in the top of the counterweight base 1 corresponding to the positions of the culture dishes 4, the culture dishes 4 can be effectively and fully contacted with the heating plate 2 in the counterweight base 1 when being placed in the placing groove 3, the culture dishes 4 can be more stably placed, a protective cover 5 is arranged above the counterweight base 1, a sealing groove 7 is formed in the bottom of the protective cover 5, a sealing ring 8 is embedded in the sealing groove 7, the sealing ring 8 can enable the sealing between the protective cover 5 and the counterweight base 1 to be better, three cavities are formed in the protective cover 5 through partition plates 6, and the 9 culture dishes 4 are arranged in a 3 x 3 manner, three cavities in the protective cover 5 are matched with the culture dish 4, the partition board 6 can divide the space in the protective covers 5 which are arranged at equal intervals on the top of the counterweight base 1, so that the culture dish 4 can be conveniently and separately arranged, the supporting rods 9 are inserted into the four corners of the top of the protective cover 5 in a penetrating and sliding mode at equal intervals, the top of each supporting rod 9 is fixedly connected with a supporting plate 10, a plurality of liquid guide boxes 11 are arranged at equal intervals on the top of each supporting plate 10 corresponding to the positions of the culture dishes 4, a first liquid inlet hopper 12 is fixedly communicated with the top of each liquid guide box 11, a fixed cover 15 is fixedly connected to the bottom of each supporting plate 10, the supporting rods 9 penetrate through the four corners of the fixed cover 15, first springs 16 are fixedly connected to the four corners of the bottom of each fixed cover 15, the first springs 16 are sleeved on the rod walls of the supporting rods 9, and one ends, far away from the fixed covers 15, of the first springs 16 are fixedly connected with the protective cover 5, the bottom of the liquid guide box 11 penetrates through one end of the support plate 10 and is fixedly communicated with a first fixed pipe 17, the inner top of the first fixed pipe 17 is fixedly connected with a first fixed ring 18, the bottom of the first fixed ring 18 is fixedly connected with a second spring 19, the other end of the second spring 19 is fixedly connected with a first liquid guide pipe 20, the first liquid guide pipe 20 is inserted in the first fixed pipe 17 in a sliding manner, a plurality of second fixed pipes 21 are fixedly inserted in the inner bottom of the fixed cover 15 at equal intervals corresponding to the position of the first fixed pipe 17, the other end of each second fixed pipe 21 penetrates through the protective cover 5, the second fixed pipes 21 are connected with the protective cover 5 in a sliding manner, a second fixed ring 22 is fixedly connected on the pipe wall of the inner top of each second fixed pipe 21, the bottom of the second fixed ring 22 is fixedly connected with a third spring 23, the other end of the third spring 23 is fixedly connected with a third fixed ring 24, the third fixed ring 24 is connected in the second fixed pipes 21 in a sliding manner, a second liquid guide tube 25 is fixedly inserted in the third fixing ring 24, a plurality of first push plates 26 and second push plates 27 are respectively inserted in the two ends and two sides of the fixing cover 15 in a sliding manner, first grooves 29 are respectively formed in the two ends of the first push plates 26 and the two sides of the second push plates 27 inserted in the fixing cover 15 in a sliding manner, fourth springs 30 are fixedly connected to the inner walls of the first grooves 29, the other ends of the fourth springs 30 are fixedly connected with the inner walls of the fixing cover 15, a plurality of second grooves 31 are formed in the tops of the first push plates 26 and the second push plates 27 at equal intervals, the second grooves 31 are arranged under the first liquid guide tube 20, the diameter of the second grooves 31 is larger than that of the first liquid guide tube 20, sealing plates 32 are fixedly connected in the second grooves 31, when the first push plates 26 and the second push plates 27 are not pushed, the sealing plates 32 prevent the first liquid guide tube 20 from being in contact with and communication with the second liquid guide tube 25, and shifting blocks 28 are fixedly connected to the two ends of the first push plates 26 and the second push plates 27, and the cross-sectional area of the toggle block 28 is greater than the cross-sectional areas of the two ends of the first push plate 26 and the second push plate 27, the toggle block 28 can make the first push plate 26 and the second push plate 27 more convenient and labor-saving when pushing, the outer wall of one side of the liquid guide box 11 is fixedly communicated with the liquid inlet pipe 13, the liquid inlet pipe 13 is arranged obliquely upwards, one end of the liquid inlet pipe 13, which is far away from the liquid guide box 11, is fixedly communicated with the second liquid inlet hopper 14, liquid to be added can be conveniently and individually added into the culture dish 4 below through the second liquid inlet hopper 14, the inner top of the first liquid guide pipe 20 is inserted with the pipe plug 33, the top of the pipe plug 33 is fixedly connected with the fixing plate 34, the fixing plate 34 is fixedly connected with the inner pipe wall of the first fixing pipe 17, and when the first liquid guide pipe 20 is contracted to the inside of the first fixing pipe 17, the pipe plug 33 can plug the first liquid guide pipe 20.
The specific culture method is that the protective cover 5 is vertically shifted upwards, the protective cover 5 is separated from the counterweight base 1, the culture dishes 4 with edible fungus strains are placed in the placing grooves 3 on the counterweight base 1, the protective cover 5 with the partition plates 6 on the counterweight base 1 can separate and protect the culture dishes 4 with different edible fungi, when culture solution is required to be added into the culture dishes 4, the corresponding liquid guide boxes 11 are opened, each liquid guide box 11 is provided with an independent second liquid inlet hopper, so that each liquid guide box 11 can be added with different dosages according to different edible fungi, meanwhile, the three adjacent liquid guide boxes 11 are provided with the first liquid inlet hopper 12 together, so that the same edible fungi are cultured together without being added independently, the culture device is more convenient to add the culture solution, the first push plate 26 and the second push plate 27 are pushed by the shifting block 28, when the first push plate 26 and the second push plate 27 are pushed, the first push plate 26 transversely moves the second push plate 27 to longitudinally move, the first push plate 26 and the second push plate 27 can drive the fourth springs 30 in the two first grooves 29 to respectively stretch and contract when moving, the elastic force of the second spring 19 can push the first liquid guide tube 20 to vertically slide downwards along the first fixing tube 17, the first liquid guide tube 20 can penetrate through the second groove 31 to extend to the inside of the second fixing tube 21 under the elastic force of the second spring 19 and be in contact communication with the second liquid guide tube 25, when one of the first push plate 26 and the second push plate 27 is not pushed, the first liquid guide tube 20 cannot extend to the inside of the second fixing tube 21 and is in communication with the second liquid guide tube 25, the situation of liquid leakage can be effectively prevented, when the second liquid guide tube 25 is pushed by the first liquid guide tube 20, the culture solution can extend to the inside of the culture dish 4 to be supplemented, make the culture solution need not to open protection casing 5 when adding, the replenishment finishes, loosens the promotion to first push pedal 26 and second push pedal 27, and first push pedal 26 and second push pedal 27 reset under the spring action of spring for first catheter 20 rises to the top of shrouding 32 along the slope limit at shrouding 32 (shrouding 32 is located second recess 31 on first push pedal 26 and second push pedal 27) both ends, closes drain box 11 simultaneously, seals the device.
Claims (9)
1. The liquid culture method of the fungi is characterized in that the culture method is used for culturing in a liquid culture device of the edible fungi, the liquid culture device of the edible fungi comprises a closed space in which a plurality of culture dishes (4) are placed, the culture dishes (4) penetrate through the closed space through corresponding liquid guide pipes and are connected with corresponding liquid guide boxes (11), the culture dishes (4) are grouped, the liquid guide boxes (11) of each group of culture dishes (4) are communicated with a first liquid inlet hopper (12), the liquid guide boxes (11) of each culture dish (4) are also connected with independent second liquid inlet hoppers, and the liquid guide pipes are opened or closed through push plates;
the culture method comprises the steps that a plurality of culture dishes (4) with different edible fungus strain culture solutions placed are placed in corresponding placing grooves (3) for culture, when the culture solutions are required to be added or oxygen is required to be supplemented, a liquid guide pipe and a liquid guide box (11) are opened, a second liquid inlet hopper (14) which is independently arranged on the liquid guide box (11) corresponding to the culture dishes (4) is used for supplementing, when the same group of culture dishes (4) are cultured together for the same edible fungi, a first liquid inlet hopper (12) is jointly arranged on three adjacent liquid guide boxes (11), and after the supplementation is finished, the liquid guide pipe and the liquid guide box (11) are closed.
2. The fungus liquid culture method according to claim 1, wherein the closed space comprises a counterweight base (1), a plurality of culture dishes (4) are placed on the top of the counterweight base (1) at equal intervals, a protective cover (5) is arranged above the counterweight base (1), a support rod (9) on the counterweight base (1) penetrates through the protective cover (5), a support plate (10) is fixedly connected to the top of the support rod, a plurality of liquid guide boxes (11) are arranged on the top of the support plate (10) at equal intervals corresponding to the positions of the culture dishes (4), and a first liquid inlet hopper (12) is fixedly communicated with the top of each liquid guide box (11).
3. The fungus liquid culture method according to claim 1, wherein the liquid guide tube comprises a first liquid guide tube (20) and a second liquid guide tube (25), one end of the bottom of the liquid guide box (11) passing through the support plate (10) is fixedly communicated with a first fixing tube (17), the inner top of the first fixing tube (17) is fixedly connected with a first fixing ring (18), the bottom of the first fixing ring (18) is fixedly connected with a second spring (19), the other end of the second spring (19) is fixedly connected with a first liquid guide tube (20), the first liquid guide tube (20) is slidably inserted into the first fixing tube (17), the inner bottom of the fixing cover (15) is fixedly inserted with a plurality of second fixing tubes (21) at equal intervals corresponding to the position of the first fixing tube (17), and the other end of the second fixing tube (21) passes through the protective cover (5), and the second fixing pipe (21) is connected with the protective cover (5) in a sliding manner, a second fixing ring (22) is fixedly connected to the pipe wall of the top in the second fixing pipe (21), a third spring (23) is fixedly connected to the bottom of the second fixing ring (22), a third fixing ring (24) is fixedly connected to the other end of the third spring (23), the third fixing ring (24) is connected to the inside of the second fixing pipe (21) in a sliding manner, and a second liquid guide pipe (25) is fixedly inserted into the inside of the third fixing ring (24).
4. The fungus liquid culture method according to claim 1, wherein the push plates comprise a first push plate (26) and a second push plate (27), two ends and two sides of the fixing cover (15) are respectively inserted with a plurality of first push plates (26) and second push plates (27) in a sliding manner, the first push plates (26) and the second push plates (27) are respectively inserted with a first groove (29) at two ends of the inside of the fixing cover (15) in a sliding manner, a fourth spring (30) is fixedly connected to the inner wall of the first groove (29), the other end of the fourth spring (30) is fixedly connected with the inner wall of the fixing cover (15), a plurality of second grooves (31) are equally spaced at the tops of the first push plates (26) and the second push plates (27), the second grooves (31) are arranged under the first liquid guide tube (20), and the diameter of the second grooves (31) is larger than that of the first liquid guide tube (20), a sealing plate (32) is fixedly connected to the inner part of the second groove (31).
5. The method for culturing a liquid fungus according to claim 2, wherein: the inside of counter weight base (1) has inlayed hot plate (2), the standing groove (3) of looks adaptation is seted up to the position that the top of counter weight base (1) corresponds culture dish (4).
6. The method for culturing a liquid fungus according to claim 2, wherein: the inside of protection casing (5) separates through baffle (6) and is equipped with three cavity, just the inside three cavity of protection casing (5) sets up with a plurality of culture dish (4) phase-match.
7. The method for culturing a liquid fungus according to claim 2, wherein: the bottom of protection casing (5) has been seted up seal groove (7), the inside scarf joint of seal groove (7) has sealing washer (8).
8. The method for culturing a liquid fungus according to claim 2, wherein: the outer wall of one side of the liquid guide box (11) is fixedly communicated with a liquid inlet pipe (13), the liquid inlet pipe (13) is arranged upwards in an inclined mode, and one end, far away from the liquid guide box (11), of the liquid inlet pipe (13) is fixedly communicated with a second liquid inlet hopper (14).
9. The method for culturing a liquid fungus according to claim 3, wherein: a pipe plug (33) is inserted into the inner top of the first liquid guide pipe (20), a fixing plate (34) is fixedly connected to the top of the pipe plug (33), and the fixing plate (34) is fixedly connected with the inner pipe wall of the first fixing pipe (17).
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| CN202110914789.1A CN113632683B (en) | 2021-08-10 | 2021-08-10 | Liquid culture method for bacteria |
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| CN202110914789.1A CN113632683B (en) | 2021-08-10 | 2021-08-10 | Liquid culture method for bacteria |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114058488A (en) * | 2021-12-15 | 2022-02-18 | 肖艳平 | Strain culture device convenient to coat based on enzyme engineering |
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| CN114058488B (en) * | 2021-12-15 | 2023-10-13 | 涌源合生科技(深圳)有限公司 | Strain culture device based on enzyme engineering is convenient for coating |
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