CN106967603B - Magnetotactic bacteria sampling culture instrument - Google Patents
Magnetotactic bacteria sampling culture instrument Download PDFInfo
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- CN106967603B CN106967603B CN201710230989.9A CN201710230989A CN106967603B CN 106967603 B CN106967603 B CN 106967603B CN 201710230989 A CN201710230989 A CN 201710230989A CN 106967603 B CN106967603 B CN 106967603B
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Abstract
The invention is suitable for the magnetotactic bacteria sampling field, particularly it is a magnetotactic bacteria sample culture apparatus, mainly used for sampling magnetotactic bacteria in the underwater soil in the lake water area in large quantities, the invention includes putting the mud box, mixing arrangement, case cover, rotating plate and sampling test tube group; put the mud case and be used for depositing the silt that the lake waters was fished out, agitating unit installs and is used for stirring mud putting the mud case lower part, and the case lid is used for sealing and for putting the mud case and providing shading strip condition putting the mud case upper end, and rotatable the arranging of rotor plate is right-hand putting the mud case, and sample test tube group evenly installs on the rotor plate, and sample test tube group is used for taking a sample to the magnetism bacterium that becomes. The magnetotactic bacteria collecting device mainly samples magnetotactic bacteria in underwater soil in a lake water area in large batch, so that the collecting efficiency of the magnetotactic bacteria collecting device is greatly improved compared with that of a traditional magnetotactic bacteria collecting mode.
Description
Technical Field
The invention is suitable for the field of magnetotactic bacteria sampling, and particularly relates to a magnetotactic bacteria sampling culture instrument.
background
Magnetotactic bacteria (Magnetotibacter) are a group of gram-negative bacteria capable of moving along the direction of a magnetic field, are widely distributed and can be found in lakes, oceans and even wet soil. The research on the formation and the magnetic properties of magnetotactic bacteria magnetosome is not only helpful for disclosing the process of biomineralization and discussing the mechanism of the biological induction geomagnetic field change, but also is an important research content of ancient geomagnetism, rock magnetics and environmental magnetics. Additionally, magnetite, a bacterial cause, also exhibits extraordinary attractiveness in modern biological and medical applications. Therefore, the research of magnetotactic bacteria has been receiving extensive attention from many disciplines since the 90 s of the 20 th century. See the literature "progress in the study of biomineralization and magnetic properties of magnetosomes of magnetotactic bacteria" [ scientific bulletin, 2004, volume 49, phase 24, p2505-2510 ]; the literature "magnetosome research on magnetosomes in magnetotactic bacteria" is published in microbiology, 2006, volume 33, phase 3, p 133-137.
The existing magnetotactic bacteria collection mode is generally as follows: firstly, collecting a sample at the junction of water and sludge in lake water, then placing the sample in a glass jar, adding a proper amount of chemical substances, and culturing at 28 ℃ in the dark for about 20 days, wherein when the surface of underwater sludge is brown, the concentration of the marker is basically finished; then, a collector is manufactured, the collecting process comprises the steps of taking a culture dish, arranging a wire mesh in the dish, taking a proper amount of the enriched sample and adding the sample into the culture dish (taking the wire mesh in the dish as the standard), then filling sterile water in the sterilized collector, covering a layer of filter paper on the opening end, quickly covering the filter paper on the wire mesh in the dish in an inverted mode, finally, placing a magnet at the bottom of the collector, enabling the S pole to face downwards, simultaneously taking a parallel mode as a control, only adding no magnet at last, collecting for about 4 hours, clamping a hose of the collector by a clamp, and collecting magnetotactic bacteria from a sampling hole by using a sterile syringe.
The prior art mainly enriches the magnetotactic bacteria in a glass jar, and then reversely buckles the magnetotactic bacteria on a culture dish through a single collector after complete enrichment; on one hand, the existing magnetotactic bacteria collection method cannot be suitable for collecting a large batch of magnetotactic bacteria, if the magnetotactic bacteria need to be collected, a plurality of groups of culture dishes and collectors need to be added, each group needs to be managed manually, or one collector and one culture dish are used for multiple times, which is very troublesome; on the other hand, the existing magnetotactic bacteria collection is performed after the magnetotactic bacteria enrichment is completed, the waiting time is long, the magnetotactic bacteria cannot be collected in the enrichment process, and much time is wasted.
In view of the above problems, the present invention provides a magnetotactic bacteria sampling culture apparatus, which has the following characteristics:
(1) The magnetotactic bacteria sampling culture instrument can sample magnetotactic bacteria in underwater soil in a lake water area in a large scale, the magnetotactic bacteria collection mode in the prior art is mainly that the magnetotactic bacteria are enriched firstly, then the magnetotactic bacteria are taken and cultured, and then test tubes are inverted and collected in a culture dish.
(2) According to the magnetotactic bacteria sampling culture instrument, once magnetotactic bacteria appear in the mud box, the magnetotactic bacteria can automatically move into the sampling test tube group to be taken, and the magnetotactic bacteria do not need to be collected after being completely enriched like a traditional mode, so that the waiting time for completely enriching the magnetotactic bacteria is shortened.
(3) According to the magnetotactic bacteria sampling culture instrument, the electromagnetic sleeve can be switched on and off through switching on and off of current, so that when magnetotactic bacteria in a test tube need to be taken out and the test tube is not required to be taken out, the electromagnetic sleeve can be demagnetized in advance, and therefore the magnetotactic bacteria in the test tube can be taken out conveniently.
(4) According to the magnetotactic bacteria sampling culture instrument, due to the design of agar in each sampling test tube group, on one hand, the process that sterile water is filled in the test tubes cannot be hindered; on the other hand, the sterile water in the test tube cannot flow out of the test tube in a large quantity to cause serious moisture on the ground and the loss of magnetotactic bacteria.
Disclosure of Invention
In order to make up for the defects of the prior art, the magnetotactic bacteria sampling culture instrument provided by the invention is mainly used for sampling magnetotactic bacteria in underwater soil in a lake water area in large batch, so that the collection efficiency of the magnetotactic bacteria is greatly improved compared with that of a traditional magnetotactic bacteria collection mode.
The technical scheme adopted by the invention for solving the technical problems is as follows: a magnetotactic bacteria sample culture apparatus, mainly used for sampling magnetotactic bacteria in the underwater soil in the lake water area on a large scale, the invention includes putting the mud box, stirring apparatus, case cover, rotating plate and sampling the test tube group; the mud box is integrally of a cubic structure with an open upper end, the mud box is used for storing silt fished out of a lake water area, a cabin door for pumping out the silt is arranged on the side wall of the mud box, the cabin door can be communicated with an external pipeline to pump the silt in the mud box, so that the trouble that a box cover is frequently opened manually to take out the silt is avoided, a sensor for detecting the height of the silt in the mud box is arranged at the upper end of the inner wall of the mud box, and a PLC (programmable logic controller) for controlling all electric elements of the mud box to automatically operate is also arranged in the mud box; the stirring device is arranged at the lower part of the sludge placing box and is used for stirring the sludge so as to avoid uneven sampling caused by uneven distribution of the sludge in the sludge placing box, if the stirring device is not designed, the sludge possibly accumulates at a higher position of the sludge placing box and accumulates at a lower position of the sludge placing box, under the condition, magnetotactic bacteria enriched at the position with lower accumulated sludge possibly cannot enter the sampling test tube group due to larger distance between the magnetotactic bacteria and the box cover, the design of the stirring device effectively avoids the defects, so that the upper end surface of the sludge in the sludge placing box can be in an approximately horizontal state no matter how much sludge is put into the sludge placing box every time, the distance between the magnetotactic bacteria enriched in each area in the sludge placing box and the sampling test tube group is approximately the same, and the sludge can move to the corresponding sampling test tube group, therefore, the design of the stirring device enables a large amount of sludge to be put into the sludge discharge box at one time, so that the sampling of a large amount of magnetotactic bacteria can be realized; the box cover is sleeved on the mud box and used for sealing the upper end of the mud box and providing shading conditions for the mud box, the enrichment treatment of magnetotactic bacteria needs to be carried out at a specific temperature in a shading way, the color of the surface of the mud can be deepened in the process until the mark enrichment is basically finished when batch brown appears, the box cover can shade the mud in the mud box, so that the enrichment treatment of the magnetotactic bacteria can be finished on the surface of the mud in the mud box, and channels through which the magnetotactic bacteria can pass are uniformly arranged on the box cover; the rotatable arrangement of rotor plate is right-hand at the mud box of putting, sample test tube group evenly install on the rotor plate, sample test tube group is used for taking a sample to magnetotactic bacterium.
Specifically, the box cover comprises a cover body, a bacteria passing pipe, a filtering membrane and a mud pouring bin; the upper end of the cover body is uniformly provided with a plurality of rows of bacteria passing holes for placing bacteria passing pipes, the bacteria passing pipes are of hollow cylindrical structures with open upper and lower ends, the bacteria passing pipes are fixedly connected in the bacteria passing holes of the cover body, the lower ends of the bacteria passing pipes are cemented with filtering membranes which only allow bacteria to pass through, and the filtering membranes are designed to prevent sludge in the sludge placing box from entering the bacteria passing pipes from the sludge placing box, so that the large pollution in the bacteria passing pipes can not be caused, a better environment is created for the subsequent sampling test tube group to sample the magnetotactic bacteria, and meanwhile, the following sterile water can not flow into the sludge placing box from the bacteria passing pipes; the upper end surface of the cover body is fixedly connected with a mud pouring bin of a cylindrical structure, sludge enters the mud discharging box through the mud pouring bin, and the upper end of the mud pouring bin is provided with a door capable of opening and closing. During operation, when needing to carry out the batch sampling to the magnetism taxis bacterium, then only need the manual work with the lid cover on putting the mud case, pour silt into from the storehouse of pouring into and put the mud case after that, the rethread puts the agitating unit in the mud case and stir silt flat can, until putting the sensor in the mud case and detecting silt height and reach logical fungus tube lower extreme filtration membrane department after, stop adding silt, silt is again put and is done the shading in the mud case and handle, silt surface can gather magnetism taxis bacterium in this in-process, magnetism taxis bacterium then can see through filtration membrane in order to move to logical fungus tube.
Specifically, a plurality of rows of water conveying channels for conveying sterile water to the bacteria-passing pipes are horizontally arranged on the cover body, the water conveying channels penetrate through the bacteria-passing holes and are communicated with the bacteria-passing pipes in the bacteria-passing holes, and valves for closing the water conveying channels are arranged at the right ends of the water conveying channels; during operation, accessible outside water pump is in order to let in the water channel with sterile water, transports through the water channel again and leads to in the fungus pipe to make the interior sterile water that is full of subsequent sample test tube group, be convenient for the magnetotactic bacterium from putting the mud box and move to the top of sample test tube group.
Specifically, a rotating plate is correspondingly arranged above each row of bacteria passing holes of the cover body, a rotating shaft driven by a motor is arranged on the right side of the sludge placing box, and the rotating plates are all positioned in the same plane and fixedly connected to the rotating shaft; and tube assembling holes for installing sampling test tube sets are formed in each rotating plate, the tube assembling holes are threaded holes, and the number and the distance of the tube assembling holes in each rotating plate are the same as those of each row of the bacteria passing holes in a one-to-one correspondence mode. During operation, the rotation of the rotating plate can be driven by controlling the rotation of the rotating shaft, and then the sampling test tube group installed on the rotating plate is driven to rotate by taking the rotating shaft as a rotating center.
Specifically, each sampling test tube group is arranged in the tube loading hole of each rotating plate and comprises a test tube, a silica gel sleeve and an electromagnetic sleeve, the diameter of the inner wall of the test tube is equal to the diameter of the inner wall of the bacteria passing tube, the design ensures that the connection part of the test tube and the bacteria passing tube is more fit, so that sterile water entering the test tube and the bacteria passing tube is prevented from seeping out from the connection part of the test tube and the bacteria passing tube, the silica gel sleeve is glued on the upper part of the test tube, the upper part of the silica gel sleeve is positioned above the upper end face of the test tube, when the test tube is reversely buckled on the bacteria passing tube, the silica gel sleeve is wrapped on the periphery of the test tube and the bacteria passing tube and plays a role in preventing water overflow, the lower part of the silica gel sleeve is provided with external threads, the test tube is connected on the tube loading hole of the rotating plate through the silica gel sleeve in a threaded manner, the test tube can be tightly nested on the rotating plate and cannot be, and threaded connection's design also is convenient for the experimenter to take off the test tube from the rotor plate, the electromagnetic sleeve inside wall be provided with the electro-magnet, the electromagnetic sleeve lateral wall passes through the screw thread and installs in dress tube hole lower part, and the electromagnetic sleeve is around the test tube periphery, the electromagnetic sleeve can provide magnetic force so that the magnetism taxis bacterium that puts the mud incasement can the automatic movement in the test tube and concentrate in the socle department of test tube, thereby realize the collection to magnetism taxis bacterium, and, the break-make of magnetic force is realized to the break-make of electromagnetic sleeve accessible electric current, and then take out the magnetism taxis bacterium in the test tube as needs, and when not wanting to take out the test tube, can make the electromagnetic sleeve lose magnetism in advance, thereby make the magnetism taxis bacterium in the.
specifically, when the rotating plate is positioned right above the cover body and is in a horizontal state, the upper end face of each bacteria passing tube on the cover body is tightly attached to the opening of a test tube of the corresponding sampling test tube group, the design is that the bacteria passing tubes and the sterile water in the test tube are difficult to seep out from the connection part of the bacteria passing tubes and the test tube, and the outer side wall of the upper end of each bacteria passing tube is wrapped by a silica gel sleeve on the test tube, so that the leakage of the sterile water is further prevented; the first of the benefit of preventing that sterile water from leading to the fungus pipe and oozing out in the test tube reduces the aseptic rivers and to subaerial volume, reduces ground humidity, and the second of the benefit makes sterile water can be very convenient be full of the test tube, is unlikely to appear still to have the condition in space between sterile water and the test tube and influences the collection of magnetotactic bacterium.
In particular, as a preferred embodiment of the present invention, each sampling tube set comprises agar, solid agar is fastened at the tube opening of each sampling tube set, and a hole for allowing sterile water and bacteria to enter the tube from the bacteria-passing tube is arranged in the middle of the agar. On one hand, the holes are formed in the middle of the agar, so that the process of filling the test tube with sterile water cannot be hindered; on the other hand, the design of the agar ensures that the sterile water in the test tube does not flow out of the test tube in a large quantity in the rotating process of the sampling test tube group, so that the ground is seriously wet and magnetotactic bacteria are not lost.
Has the advantages that:
(1) The magnetotactic bacteria sampling culture instrument can sample magnetotactic bacteria in underwater soil in a lake water area in a large scale, the magnetotactic bacteria collection mode in the prior art is mainly that the magnetotactic bacteria are enriched firstly, then the magnetotactic bacteria are taken and cultured, and then test tubes are inverted and collected in a culture dish.
(2) According to the magnetotactic bacteria sampling culture instrument, once magnetotactic bacteria appear in the mud box, the magnetotactic bacteria can automatically move into the sampling test tube group to be taken, and the magnetotactic bacteria do not need to be collected after being completely enriched like a traditional mode, so that the waiting time for completely enriching the magnetotactic bacteria is shortened.
(3) According to the magnetotactic bacteria sampling culture instrument, the electromagnetic sleeve can be switched on and off through switching on and off of current, so that when magnetotactic bacteria in a test tube need to be taken out and the test tube is not required to be taken out, the electromagnetic sleeve can be demagnetized in advance, and therefore the magnetotactic bacteria in the test tube can be taken out conveniently.
(4) According to the magnetotactic bacteria sampling culture instrument, due to the design of agar in each sampling test tube group, on one hand, the process that sterile water is filled in the test tubes cannot be hindered; on the other hand, the sterile water in the test tube cannot flow out of the test tube in a large quantity to cause serious moisture on the ground and the loss of magnetotactic bacteria.
Drawings
The invention is further described with reference to the following figures and embodiments.
FIG. 1 is a top view of the present invention;
FIG. 2 is a sectional view taken along the line A-A of the present invention;
FIG. 3 is a sectional view taken along the line B-B of the present invention;
FIG. 4 is a schematic view of the rotational path of the rotating plate of the present invention;
FIG. 5 is a schematic view of a sampling tube set according to the present invention;
In the figure: the device comprises a mud discharging box 1, a stirring device 2, a box cover 3, a rotating plate 4, a sampling test tube group 5, a cover body 31, a water conveying channel 311, a bacteria introducing tube 32, a filtering membrane 33, a mud pouring bin 34, a tube loading hole 41, a test tube 51, a silica gel sleeve 52, an electromagnetic sleeve 53 and agar 54.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the following embodiments.
as shown in figures 1 and 2, the magnetotactic bacteria sampling culture instrument is mainly used for sampling magnetotactic bacteria in underwater soil in a lake water area in large batch, and comprises a sludge placing box 1, a stirring device 2, a box cover 3, a rotating plate 4 and a sampling test tube group 5; the mud box 1 is integrally of a cubic structure with an open upper end, the mud box 1 is used for storing silt fished out from a lake water area, a cabin door for pumping out the silt is arranged on the side wall of the mud box 1 and can be communicated with an external pipeline to pump the silt in the mud box 1, so that the trouble that a box cover 3 is frequently opened manually to take out the silt is avoided, a sensor for detecting the height of the silt in the mud box 1 is arranged at the upper end of the inner wall of the mud box 1, and a PLC (programmable logic controller) for controlling all electric elements of the mud box 1 to automatically operate is also arranged in the mud box 1; the stirring device 2 is arranged at the lower part of the sludge placing box 1 and is used for stirring the sludge so as to avoid uneven sampling caused by uneven distribution of the sludge in the sludge placing box 1, if the stirring device 2 is not designed, the sludge possibly accumulates at a higher position of the sludge placing box 1, and accumulates at other positions of the sludge placing box 1 at a lower position, under the condition, magnetotactic bacteria enriched at the position with lower accumulated sludge possibly cannot enter the sampling test tube group 5 due to a larger distance between the magnetotactic bacteria and the box cover 3, the design of the stirring device 2 effectively avoids the defects, so that the upper end surface of the sludge in the sludge placing box 1 can be in an approximately horizontal state no matter how much sludge is thrown into the sludge placing box 1 every time, and the distance between the magnetotactic bacteria enriched in each area in the sludge placing box 1 and the sampling test tube group 5 is approximately the same and the corresponding sampling test tube group can move into the corresponding sampling test tube group 5, therefore, the design of the stirring device 2 enables a large amount of sludge to be put into the sludge discharge box 1 at one time, so that the sampling of a large amount of magnetotactic bacteria can be realized, and the stirring device 2 can be a combination of a motor and a stirring rod or a stirring blade, but is not limited to the combination; the box cover 3 is sleeved on the mud box 1, the box cover 3 is used for sealing the upper end of the mud box 1 and providing shading conditions for the mud box 1, the enrichment treatment of magnetotactic bacteria needs shading culture at a specific temperature, the marker enrichment is basically completed when the surface color of the mud is deepened in the process until batch brown appears, the box cover can shade the mud in the mud box 1, so that the surface of the mud in the mud box 1 can complete the enrichment treatment of magnetotactic bacteria, and channels through which the magnetotactic bacteria can pass are uniformly arranged on the box cover 3; the rotating plate 4 is rotatably arranged on the right side of the mud box 1, the sampling test tube groups 5 are uniformly arranged on the rotating plate 4, and the sampling test tube groups 5 are used for sampling magnetotactic bacteria.
Specifically, as shown in fig. 1, fig. 2, fig. 3 and fig. 4, the case cover 3 of the magnetotactic bacteria sampling culture apparatus of the present invention includes a cover body 31, a bacteria-passing tube 32, a filter membrane 33 and a mud pouring bin 34; the upper end of the cover body 31 is uniformly provided with a plurality of rows of bacteria passing holes for placing the bacteria passing pipes 32, the bacteria passing pipes 32 are of hollow cylindrical structures with open upper and lower ends, the bacteria passing pipes 32 are fixedly connected in the bacteria passing holes of the cover body 31, the lower ends of the bacteria passing pipes 32 are glued with filter membranes 33 which only allow bacteria to pass, and the filter membranes 33 are designed to prevent sludge in the sludge placing box 1 from entering the bacteria passing pipes 32 from the sludge placing box 1, so that the large pollution in the bacteria passing pipes 32 can not be caused, a better environment is created for the subsequent sampling test tube group 5 to sample magnetotactic bacteria, and meanwhile, the following sterile water can not flow into the sludge placing box 1 from the bacteria passing pipes 32; the upper end surface of the cover body 31 is fixedly connected with a mud pouring bin 34 with a cylindrical structure, mud enters the mud discharging box 1 through the mud pouring bin 34, and the upper end of the mud pouring bin 34 is provided with a door capable of opening and closing. During operation, when magnetotactic bacteria need to be sampled in batches, only the cover body 31 needs to be manually sleeved on the sludge placing box 1, then sludge is poured into the sludge placing box 1 from the sludge pouring bin 34, and then the sludge is stirred by the stirring device 2 in the sludge placing box 1, until a sensor in the sludge placing box 1 detects that the height of the sludge reaches the filtering membrane 33 at the lower end of the bacteria passing tube 32, the sludge is stopped to be added, then the sludge is subjected to shading treatment in the sludge placing box 1, magnetotactic bacteria can be gathered on the surface of the sludge in the process, and the magnetotactic bacteria can move into the bacteria passing tube 32 through the filtering membrane 33.
Specifically, as shown in fig. 1, fig. 2 and fig. 4, in the magnetotactic bacteria sampling incubator of the present invention, a plurality of rows of water channels 311 for conveying sterile water to the bacteria-passing tubes 32 are horizontally disposed on the cover 31, the water channels 311 penetrate through the bacteria-passing holes and are communicated with the bacteria-passing tubes 32 in the bacteria-passing holes, and a valve for closing the water channels 311 is disposed at the right end of the water channels 311; during operation, the external water pump can be used for introducing sterile water into the water delivery channel 311 and then delivering the sterile water into the bacteria-passing pipe 32 through the water delivery channel 311, so that the subsequent sampling test tube group 5 is filled with the sterile water, and magnetotactic bacteria can move to the bottom of the sampling test tube group 5 from the mud-discharging box 1 conveniently.
Specifically, as shown in fig. 1, fig. 2 and fig. 5, a rotating plate 4 is correspondingly arranged above each row of bacteria-passing holes of the cover 31, a rotating shaft driven by a motor is arranged on the right side of the mud-placing box 1, and the rotating plates 4 are all in the same plane and are fixedly connected to the rotating shaft; each rotating plate 4 is provided with a tube installing hole 41 for installing the sampling test tube group 5, the tube installing holes 41 are threaded holes, and the number and the distance of the tube installing holes 41 on each rotating plate 4 are the same as those of each row of bacteria passing holes in a one-to-one correspondence manner. During operation, the rotation of the rotating shaft can be controlled to drive the rotating plate 4 to rotate, and then the sampling tube group 5 mounted on the rotating plate 4 is driven to rotate by taking the rotating shaft as a rotating center.
Specifically, as shown in fig. 2 and 5, in the magnetotactic bacteria sampling incubator of the present invention, a sampling tube set 5 is installed in the tube-installing hole 41 of each rotating plate 4, each sampling tube set 5 includes a test tube 51, a silicone sleeve 52 and an electromagnetic sleeve 53, the diameter of the inner wall of the test tube 51 is equal to the diameter of the inner wall of the bacteria-passing tube 32, the design is such that the connection between the test tube 51 and the bacteria-passing tube 32 is more engaged, so as to help prevent the sterile water entering the test tube 51 and the bacteria-passing tube 32 from leaking out from the connection between the test tube 51 and the bacteria-passing tube 32, the silicone sleeve 52 is glued on the upper portion of the test tube 51, the upper portion of the silicone sleeve 52 is located above the upper end surface of the test tube 51, when the test tube 51 is turned over the bacteria-passing tube 32, the silicone sleeve 52 wraps around the peripheries of the test tube 51 and the bacteria-passing tube 32 to prevent the water from overflowing, the lower portion of the silicone sleeve 52 is provided with external threads, the test tube 51 is screwed on the tube-, the design of the threaded connection enables the test tube 51 to be tightly nested on the rotating plate 4 so as not to change position or even fall off in the process of rotating along with the rotating plate 4, the design of the threaded connection is convenient for an experimenter to take the test tube 51 off the rotating plate 4, an electromagnet is arranged on the inner side wall of the electromagnetic sleeve 53, the outer side wall of the electromagnetic sleeve 53 is installed on the lower portion of the tube containing hole 41 through threads, the electromagnetic sleeve 53 is wrapped on the periphery of the test tube 51, the electromagnetic sleeve 53 can provide magnetic force so that magnetotactic bacteria in the mud discharging box 1 can automatically move into the test tube 51 and are intensively located at the bottom of the test tube 51, thereby realizing the collection of the magnetotactic bacteria, the electromagnetic sleeve 53 can realize the on-off of the magnetic force through the on-off of the current, and further when the magnetotactic bacteria in the test tube 51 need to be taken out and the test tube 51 is not needed to be, thereby allowing the magnetotactic bacteria in the test tube 51 to be easily removed.
specifically, when the rotating plate 4 is located right above the cover 31 and is in a horizontal state, the upper end surface of each bacteria-passing tube 32 on the cover 31 is closely attached to the opening of the test tube 51 of the corresponding sampling tube set 5, the design is such that the sterile water in the bacteria-passing tubes 32 and the test tubes 51 is difficult to seep out from the connection between the bacteria-passing tubes 32 and the test tubes 51, the outer side wall of the upper end of the bacteria-passing tubes 32 is wrapped by the silicone sleeve 52 on the test tubes 51, and the design is such that the seepage of the sterile water is further prevented; one of the benefits of preventing the leakage of the sterile water from the bacteria-passing tube 32 and the test tube 51 is to reduce the amount of the sterile water flowing to the ground and reduce the humidity of the ground, and the other benefit is to make the sterile water conveniently fill the test tube 51, so that the situation that a space exists between the sterile water and the test tube 51 to affect the collection of the magnetotactic bacteria does not occur.
Specifically, as shown in fig. 5, in the magnetotactic bacteria sampling incubator of the present invention, as a preferred embodiment of the present invention, each sampling tube set 5 comprises agar 54, the opening of the test tube 51 of each sampling tube set 5 is fastened with solid agar 54, and the middle of the agar 54 is provided with a hole for allowing sterile water and bacteria to enter the test tube 51 from the bacteria-passing tube 32. On one hand, the holes are arranged in the middle of the agar, so that the process that the test tube 51 is filled with sterile water cannot be hindered; on the other hand, the agar is designed so that the sterile water in the test tube 51 will not flow out of the test tube 51 in a large amount during the rotation of the sampling tube set 5, which results in severe moisture on the ground and magnetotactic bacteria loss.
In an initial state, the rotating plate 4 is positioned at the upper right of the sludge placing box 1, the sampling tube group 5 on the rotating plate 4 is in a vertical state, the openings of the test tubes 51 are upward, when the magnetic bacteria are required to be collected and sampled in a large batch, firstly, the cover body 31 is sleeved on the sludge placing box 1 manually, then, sludge is poured into the sludge placing box 1 from the sludge pouring bin 34, a proper amount of chemical substances are added (the chemical substances are well known in the field of the existing magnetic bacteria and are not described here), then, the sludge is stirred evenly by the stirring device 2 in the sludge placing box 1, the sludge is stopped to be added until the height of the sludge reaches the filtering membrane 33 at the lower end of the bacteria passing tube 32 detected by the sensor in the sludge placing box 1, then, light shielding treatment is carried out on the sludge in the sludge placing box 1, and the magnetic bacteria are gathered on the surface of the sludge in the process.
Then, the rotating plate rotates 180 degrees to make the test tube opening of each sampling test tube group fit with the upper end of each corresponding bacteria-passing tube on the box cover 3, then sterile water is pumped into the water delivery channel 311 by an external water pump and is conveyed into the bacteria-passing tube 32 through the water delivery channel 311, so that the sampling test tube group 5 is filled with the sterile water, then the magnetotactic bacteria accumulated on the surface of the sludge box 1 move to the bacteria-passing tube 32 from the sludge box 1 and move to the tube bottom of the sampling test tube group 5 from the bacteria-passing tube 32, under the magnetic force of the peripheral electromagnetic sleeve of the sampling test tube group 5, the magnetotactic bacteria are gathered to the tube bottom of the sampling test tube group 5, then, the rotating plate 4 rotates 180 degrees reversely, so that the test tube 51 of the sampling test tube group 5 in which the magnetotactic bacteria are collected faces upwards, and then the magnetotactic bacteria in each sampling test tube group 5 are taken out.
Repeating the above actions repeatedly to realize the batch collection and sampling of magnetotactic bacteria in the sludge discharge box 1; the magnetotactic bacteria batch collection sampling device is inseparable through the matching operation of the sludge discharge box 1, the stirring device 2, the box cover 3, the rotating plate 4 and the sampling test tube group 5, and realizes the batch collection sampling of magnetotactic bacteria.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. The utility model provides a magnetotactic bacteria sample culture appearance, the magnetic tactic bacteria who mainly is arranged in carrying out the bulk sampling to the soil under water in lake water area, its characterized in that: comprises a mud box (1), a stirring device (2), a box cover (3), a rotating plate (4) and a sampling test tube group (5); the mud placing box (1) is integrally of a cubic structure with an open upper end, the mud placing box (1) is used for storing mud fished out of a lake water area, and a cabin door for pumping out the mud is arranged on the side wall of the mud placing box (1); the stirring device (2) is arranged at the lower part of the sludge discharge box (1) and is used for stirring sludge so as to avoid uneven sampling caused by uneven distribution of the sludge in the sludge discharge box (1); the box cover (3) is sleeved on the mud box (1), the box cover (3) is used for sealing the upper end of the mud box (1) and providing a light shielding condition for the mud box (1), and channels through which magnetotactic bacteria can pass are uniformly arranged on the box cover (3); the rotating plate (4) is rotatably arranged on the right side of the sludge placing box (1), the sampling test tube groups (5) are uniformly arranged on the rotating plate (4), and the sampling test tube groups (5) are used for sampling magnetotactic bacteria;
The box cover (3) comprises a cover body (31), a bacteria passing pipe (32), a filter membrane (33) and a mud pouring bin (34); a plurality of rows of bacteria passing holes for placing the bacteria passing pipes (32) are uniformly formed in the upper end of the cover body (31), the bacteria passing pipes (32) are of hollow cylindrical structures with open upper and lower ends, the bacteria passing pipes (32) are fixedly connected in the bacteria passing holes of the cover body (31), and the lower ends of the bacteria passing pipes (32) are cemented with filtering membranes (33) which only allow bacteria to pass through; the upper end surface of the cover body (31) is fixedly connected with a mud pouring bin (34) with a cylindrical structure, sludge enters the mud discharging box (1) through the mud pouring bin (34), and the upper end of the mud pouring bin (34) is provided with a door capable of being opened and closed;
A plurality of rows of water conveying channels (311) for conveying sterile water to the bacteria passing pipes (32) are horizontally arranged on the cover body (31), the water conveying channels (311) penetrate through the bacteria passing holes and are communicated with the bacteria passing pipes (32) in the bacteria passing holes, and a valve for closing the water conveying channels (311) is arranged at the right end of each water conveying channel (311);
A rotating plate (4) is correspondingly arranged above each row of bacteria passing holes of the cover body (31), a rotating shaft driven by a motor is arranged on the right side of the sludge placing box (1), and the rotating plates (4) are all positioned in the same plane and fixedly connected to the rotating shaft; tube installing holes (41) for installing sampling test tube sets (5) are formed in the rotating plates (4), the tube installing holes (41) are threaded holes, and the number and the intervals of the tube installing holes (41) in the rotating plates (4) are the same as those of the bacteria passing holes in each row in a one-to-one correspondence manner;
all install sample test tube group (5) in dress tube hole (41) of each rotor plate (4), each sample test tube group (5) all includes test tube (51), silica gel cover (52) and electromagnetic sleeve (53), test tube (51) inner wall diameter equal with logical fungus pipe (32) inner wall diameter, silica gel cover (52) gluing on test tube (51) upper portion, and silica gel cover (52) upper portion is located test tube (51) up end top, silica gel cover (52) lower part is provided with the external screw thread, test tube (51) pass through silica gel cover (52) threaded connection on dress tube hole (41) of rotor plate (4), electromagnetic sleeve (53) inside wall be provided with the electro-magnet, electromagnetic sleeve (53) lateral wall passes through the screw thread and installs dress tube hole (41) lower part, and electromagnetic sleeve (53) wraps around test tube (51) periphery.
2. The magnetotactic bacteria sampling incubator of claim 1, wherein: when rotor plate (4) were located directly over lid (31) and were in the horizontality, each logical fungus pipe (32) up end on lid (31) all with correspond test tube (51) opening part of sample test tube group (5) and closely laminate, silica gel cover (52) on test tube (51) are wrapped and are lived logical fungus pipe (32) upper end lateral wall.
3. the magnetotactic bacteria sampling incubator of claim 1, wherein: each test tube group (5) of taking a sample all includes agar (54), and the test tube (51) opening part fastening of each test tube group (5) of taking a sample has solid agar (54), and agar (54) middle part is provided with the hole that gets into in test tube (51) from leading to bacterin pipe (32) sterile water and bacterium of being convenient for.
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| CN117247836B (en) * | 2023-11-20 | 2024-02-02 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
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| CN1880441A (en) * | 2006-05-12 | 2006-12-20 | 中国科学院地球化学研究所 | Magnetic bacteria fast-collection device |
| CN101671631A (en) * | 2009-10-22 | 2010-03-17 | 中南大学 | Magnetic bacteria separometer |
| WO2011096659A2 (en) * | 2010-02-05 | 2011-08-11 | Jeon Min-Yong | Microorganism or cell culture container |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880441A (en) * | 2006-05-12 | 2006-12-20 | 中国科学院地球化学研究所 | Magnetic bacteria fast-collection device |
| CN101671631A (en) * | 2009-10-22 | 2010-03-17 | 中南大学 | Magnetic bacteria separometer |
| WO2011096659A2 (en) * | 2010-02-05 | 2011-08-11 | Jeon Min-Yong | Microorganism or cell culture container |
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