CN110972942B - Intelligent seedling breeding method - Google Patents

Intelligent seedling breeding method Download PDF

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Publication number
CN110972942B
CN110972942B CN201911288812.XA CN201911288812A CN110972942B CN 110972942 B CN110972942 B CN 110972942B CN 201911288812 A CN201911288812 A CN 201911288812A CN 110972942 B CN110972942 B CN 110972942B
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seed
tissue culture
suspension
liquid
bottle
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CN110972942A (en
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郑延海
王海兰
陈凯
陈傲然
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Fujian Sanan Sino Science Photobiotech Co Ltd
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Fujian Sanan Sino Science Photobiotech Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like

Abstract

The invention discloses an intelligent seedling breeding method, which comprises the steps of (1) preparing a seed suspension with specific seed density and specific agar concentration; (2) loading the seed suspension into a closed seed suspension germination accelerating tank; (3) uniformly stirring the seed suspension in the closed seed suspension germination accelerating tank by using a stirrer; (4) carry the seed suspension of stirring to the seed liquid box of keeping in with seed liquid pipeline, the portable seeding probe on the automatic dibbler of seed rain slides along the slide rail under drive arrangement's effect, changes portable seeding probe and slides to the seed liquid box top of keeping in, and then moves down under drive arrangement and dip in the seed suspension in the seed liquid box of keeping in, (5) portable seeding probe moves the group banks up bottle top with earth under drive arrangement effect, this seed suspension evenly dibbles with the seed rain mode on the culture medium of group banks up the bottle with earth. The invention has the advantages of high working efficiency, less pollution, low production cost and standardization.

Description

Intelligent seedling breeding method
Technical Field
The invention relates to the field of agricultural planting, in particular to an intelligent seedling breeding method.
Background
With the continuous development of scientific technology, more and more automatic technologies are applied to the field of agricultural planting, and the technologies are used for reducing the labor cost required for planting crops, improving the working efficiency and reducing the production cost. The plant tissue culture technology is a new traditional Chinese medicine seedling rapid propagation technology developed in recent years, is a technology for obtaining a regenerated complete plant by inoculating tissues, organs and the like of a plant on a culture medium containing various nutrient substances and phytohormones under an aseptic condition through aseptic operation, and has the characteristics of high propagation efficiency, short period and regular and consistent seedlings. The prior Chinese medicinal material tissue culture and rapid propagation technology has the following problems: 1) the tissue culture seedling inoculation equipment is simple, the system integration level is low, the compactness is poor, a large number of high-quality technical workers are needed in each link, the inoculation quality and the inoculation speed are guaranteed, and the labor cost is high; 2) the seedling raising system has high frame manufacturing cost and very low intellectualization degree, is difficult to form a seedling raising and planting system with unified standard, all links also need manual control, and the quality of inoculated seedlings is uneven due to different technical proficiency degrees of operators. 3) The operation link is complicated and inconvenient, the production efficiency is low, and the production cost is high.
Disclosure of Invention
Based on the defects in the prior art, the invention provides an intelligent seedling breeding system. Through improving the structure of whole group banks up the system with earth, adopt the liquid of innovation to carry and the dibble seeding mode, realize the accurate supply of the nutrient solution of growing seedlings, greatly improved the efficiency of growing seedlings to resource saving has been played, pollution abatement's beneficial effect.
In order to achieve the purpose, the invention adopts the technical scheme that: the utility model provides an intelligent seedling breeding system, includes germination accelerating tank, seed liquid pipeline, the automatic dibbler of seed rain and group banks up the bottle with earth, the automatic dibbler of seed rain includes the organism, be equipped with the slide rail on the organism, it is equipped with portable seeding probe to slide on the slide rail, portable seeding probe slides along the track, still be equipped with the seed liquid box of keeping in the organism, group banks up the bottle with earth and is located slide rail and seeding probe below, germination accelerating tank passes through seed liquid pipeline and seed liquid box intercommunication of keeping in, the relative slide rail up-and-down motion of portable seeding probe, portable seeding probe slides to group banks up the bottle top from seed liquid box top of keeping in on the track, portable seeding probe dips in the seed liquid of getting in the seed liquid box of keeping in and dibbles to group banks up in the bottle with earth.
Further, the germination accelerating tank is a closed seed suspension germination accelerating tank.
Further, the seed liquid is kept in box top height and is less than germination accelerating tank top height in the seed liquid, just seed liquid conveying pipeline stretches out from germination accelerating tank top, extends to the seed liquid and keeps in box top and insert in the seed liquid box of keeping in downwards along the horizontal direction. The seed liquid conveying pipeline has a design structure, so that when the liquid level in the box is lower than a certain height, the seed suspension liquid is automatically supplemented from the closed seed suspension liquid germination accelerating tank under the action of atmospheric pressure.
Further comprises a first driving device which is arranged on the machine body and drives the movable sowing probe to slide along the length direction of the slide rail,
and the movable seeding probe is driven to move up and down relative to the sliding rail by the second driving device arranged on the machine body.
Further, still include a conveyer belt, the conveyer belt sets up in track and portable seeding probe below for carry group to organize the bottle.
Furthermore, the tissue culture bottle is arranged on the tissue culture basket, and a friction layer for enhancing the friction force between the tissue culture bottle and the conveyor belt is arranged at the bottom of the tissue culture basket. A limiting mechanism used for limiting the position of the tissue culture bottle is arranged in the tissue culture basket.
Further, still include bottle capper, the bottle capper sets up the end at conveyer belt direction of transfer, the bottle capper is group banks up the lid for group when the conveyer belt will bank up the group with the group the bottle and convey the end.
Furthermore, a culture medium is arranged in the tissue culture bottle.
Further, the germination accelerating tank is communicated with the oxygen adding device through an oxygen conveying pipeline. And adjusting the dissolved oxygen of the seed suspension in the germination accelerating tank by an oxygen adding device.
Further, the device comprises a temperature control device, wherein the temperature control device is arranged at the bottom of the germination accelerating tank and is used for adjusting the temperature of the planting suspension. The temperature control device is specifically an electromagnetic heating device or an electric heating wire heater.
Furthermore, the seed rain automatic dibbler also comprises a seed liquid volume quantitative setting controller, so as to control the volume of the liquid drops dibbled onto the culture medium by the movable sowing probe according to the concentration of the seed suspension and the seed number demand in the liquid drops.
The invention also discloses an intelligent seedling breeding method which is realized by adopting the intelligent seedling breeding system and comprises the following steps:
(1) preparing a seed suspension with specific seed density and specific agar concentration;
(2) loading the seed suspension into a closed seed suspension germination accelerating tank;
(3) uniformly stirring the seed suspension in the closed seed suspension germination accelerating tank by using a stirrer;
(4) the seed suspension liquid which is evenly stirred is conveyed to a seed liquid temporary storage box by a seed liquid conveying pipeline, a movable seeding probe on the automatic seed rain dibbler slides along a slide rail under the action of a driving device, the movable seeding probe slides to the upper part of the seed liquid temporary storage box, and then the seed suspension liquid in the seed liquid temporary storage box is dipped by downward movement under the action of the driving device,
(5) the movable sowing probe moves to the upper part of the tissue culture bottle under the action of the driving device, and the seed suspension is evenly dibbled to a culture medium in the tissue culture bottle in a seed rain mode.
And (3) further conveying the tissue culture bottles after the seeds are sown in the step (6) to a bottle covering machine through a conveying belt, covering the bottle covers and placing the bottle covers on a culture rack with a proper light source.
Furthermore, the density range of the specific seeds is 100-3000 grains/mL, and preferably 150-250 grains/mL.
Furthermore, the concentration range of the specific agar is 1-10 g/L, which refers to the mass of the agar in a unit volume of solution.
Further, in the step (2), the temperature in the germination accelerating tank is controlled to be 23-26 ℃, and the oxygen content is controlled to be 5-8 mg/l.
Further, in the step (4), when the seeds exposed to the white in the germination accelerating tank are conveyed to the seed liquid temporary storage box through the seed liquid conveying pipeline, the seed suspension liquid which is uniformly stirred is conveyed to the seed liquid temporary storage box.
Further, in the step (5), the seeds are sown in a seed rain mode by controlling the density of the liquid drops to be 0.15-0.25 drops/cm 2 and the number of the seeds per drop to be 2-3.
The invention has the beneficial effects that:
the application of the intelligent seedling raising system reduces the operation links, and has the advantages of high working efficiency, less pollution, low production cost and standardization.
The invention replaces the full-artificial inoculation seedling breeding technology with intelligent equipment, greatly reduces the labor cost and improves the seedling quality consistency through standardized operation.
The tissue culture container used in the invention is a cylindrical bottle, the diameter of the upper cover is slightly larger than that of the lower body, so that the tissue culture container can be tightly covered and sealed, and the upper cover is made of a material with high light transmittance, so that the waste of light sources can be reduced to the maximum extent, and the light energy utilization rate and the growth rate of tissue culture seedlings can be improved.
Drawings
Fig. 1 is a schematic diagram of the intelligent seedling breeding system of this embodiment.
FIG. 2 is a schematic structural view of the seed rain automatic dibbler of the present embodiment.
Fig. 3 is a schematic structural diagram of the first driving device of the present embodiment.
Fig. 4 is a schematic structural diagram of a second driving device according to the present embodiment.
Description of reference numerals: 1. temperature control device, 2, vernalization jar, 3, agitator, 4, oxygenation device, 5, seed liquid conveying line, 6, oxygen conveying line, 7, the automatic dibbler organism of seed rain, 8, the seed liquid box of keeping in, 9, slide rail, 10, portable seeding probe, 11, group banks up the bottle with earth, 12 group banks up the basket with earth, 13, conveyer belt, 14, bottle lid machine, 15, bottle lid, 16, first drive arrangement, 17, second drive arrangement.
Detailed Description
The invention is further described with reference to the following drawings and detailed description.
An intelligent seedling breeding system is shown in fig. 1 and fig. 2. The utility model provides an intelligent seedling breeding system, includes temperature control device 1, vernalization jar 2 is closed seed suspension vernalization jar 2, and seed liquid pipeline 5, the automatic dibbler of seed rain, group banks up bottle 11, group and organizes basket 12, conveyer belt 13, bottle lid machine 14 with earth, the automatic dibbler of seed rain includes organism 7, be equipped with slide rail 9 on the organism 7, slide on slide rail 9 and be equipped with portable seeding probe, portable seeding probe slides along slide rail 9, and the automatic dibbler of seed rain still includes first drive arrangement 16 and second drive arrangement 17, first drive arrangement 16 sets up on organism 7, and the portable seeding probe of drive slides along slide rail 9 length direction. Referring specifically to fig. 3, the first driving device 16 includes a driving motor 161 and a driving transmission belt 161, the driving transmission belt 161 is disposed in the sliding rail 9, a sliding block 101 is disposed above the movable sowing probe 10, a sliding gear 102 is disposed on the sliding block 101, the sliding gear 102 is engaged with the driving transmission belt 161, the driving transmission belt 161 is driven to rotate by the rotation of the driving motor 161, and the sliding gear 101 is driven to rotate, so that the movable sowing probe 10 horizontally slides on the sliding rail 9. The second driving device 17 is arranged on the machine body 7 and drives the movable sowing probe to move up and down relative to the slide rail 9. The second driving device 17 comprises a telescopic cylinder 171, the telescopic cylinder 171 is fixed on the movable sowing probe 10, and the output end of the telescopic cylinder 171 passes through the slide block 101 of the movable sowing probe 10 and can drive the bottom dipping block 102 to move up and down.
The germination accelerating tank 2 is communicated with the oxygen adding device 4 through an oxygen conveying pipeline 6. The dissolved oxygen of the seed suspension in the germination accelerating tank 2 is adjusted by an oxygen adding device.
Still be equipped with seed liquid in the organism 7 and keep in box 8, 8 top heights of box are kept in to seed liquid are less than 2 top heights of vernalization jar, just 5 ways of seed liquid conveyer pipe stretch out from 2 tops of vernalization jar, extend to 8 tops of box are kept in to seed liquid and insert in the box 8 is kept in to seed liquid downwards along the horizontal direction. The seed liquid conveying pipe 5 is designed in a structure, so that when the liquid level in the box is lower than a certain height, the seed suspension is automatically supplemented from the closed seed suspension germination accelerating tank 2 under the action of atmospheric pressure. Tissue culture bottle 11 is located slide rail 9 and seeding probe below, germinating tank 2 keeps in box 8 intercommunication through 5 ways of seed liquid conveyer pipe and seed liquid, the relative slide rail 9 up-and-down motion of portable seeding probe, portable seeding probe slides to tissue culture bottle 11 top from 8 tops of the box of keeping in of seed liquid on the track, portable seeding probe dips in the seed liquid of getting in the box 8 of seed liquid and dibble seeding to tissue culture in the bottle 11. The tissue culture bottle 11 is internally provided with a culture medium.
The conveyer belt 13 sets up in track and portable seeding probe below for carry group and organize bottle 11 with earth. Tissue culture bottle 11 sets up on tissue culture basket 112, tissue culture basket 12 bottom is equipped with the frictional layer of reinforcing and conveyer belt frictional force. A limiting mechanism used for limiting the position of the tissue culture bottle 11 is arranged in the tissue culture basket 112.
The bottle capper 14 sets up the end at conveyer belt direction of transfer, the bottle capper is group banks up lid for group with group when organizing bottle 11 to convey the end with group at the conveyer belt for group banks up bottle 11 with earth.
The temperature control device is arranged at the bottom of the germination accelerating tank 2 and is used for adjusting the temperature of the planting suspension. The temperature control device 1 is specifically an electromagnetic heating device or a heating wire heater, or the temperature control device 1 adopts a temperature sensor, an MCU and an electromagnetic heating device, the temperature sensor collects temperature information and sends the temperature information to the MCU, the MCU generates a control signal and sends the control signal to the electromagnetic heating device, and the electromagnetic heating device receives the control signal to start or close an electromagnetic heating function and control electromagnetic heating time.
The seed rain automatic dibbler also comprises a seed liquid volume quantitative setting controller, so as to control the volume of the liquid drops dibbled onto the culture medium by the movable sowing probe according to the concentration of the seed suspension and the seed number demand in the liquid drops. The volume setting method comprises the following steps: taking dendrobium as an example, 20 ten thousand dendrobium seeds are added into 1L of liquid with agar concentration of 1-3 g/L, namely, a uniform seed suspension with concentration of 0.2 grains/microliter is generated. Then, the movable sowing probe is controlled to dibble the liquid drop on the culture medium with a volume of 10 microliter according to the concentration of the seed suspension and the seed number requirement (e.g. 2 grains/drop) in the liquid drop.
According to the device provided by the embodiment of the invention, the closed seed suspension germination accelerating tank is arranged, so that the optimum condition for seed germination accelerating is perfectly realized, the germination vigor and consistency are improved, the cleanliness of the inner space of the germination tank is effectively ensured, and the pollution is reduced. The homogeneity of the seed suspension is achieved by adjusting the agar content of the liquid, which ensures that a relatively consistent "seed rain" is obtained. Oxygen adding pump for ensuring proper concentration of O in seed suspension2The content can provide enough oxygen required by seed germination, thereby improving the germination vigor and the germination rate to the maximum extent and optimizing the quality of a seed suspension.
The embodiment of the invention utilizes the characteristic that partial Chinese medicinal material seeds can be subjected to suspension liquid germination acceleration, obtains the suspension liquid with uniformly distributed seeds by adjusting the agar concentration of the suspension liquid and properly stirring, uniformly seeds the suspension liquid with germination acceleration and white exposure to a culture medium in tissue culture bottles through intelligent seed rain dibbling equipment, accurately controls the density of liquid drops, and basically keeps the number of seeds in each liquid drop consistent, so that the number of seeds in each tissue culture bottle is basically consistent, the sowed tissue culture bottle is covered and placed on a culture frame, and high-quality seedlings are obtained through meticulous culture for a certain time.
By adopting the technical scheme in the embodiment, the following technical effects are achieved:
1) the seed germination accelerating device is relatively closed, is clean and environment-friendly, and effectively avoids germ pollution;
2) the buoyancy of the seed suspension is adjusted by changing the agar concentration of the solution, and then the uniform distribution of the seeds in the solution is realized by stirring;
3) the intelligent seed rain dibbler realizes the uniform distribution of seed liquid drops and the basic consistency of the number of seeds in each tissue culture bottle;
4) the transmission frequency of the tissue culture bottle after seeding is precisely consistent with the working frequency of the bottle capping machine, so that the two devices are well matched;
5) the tissue culture bottle covered with the cover is placed on a clean culture room culture shelf, and strong, tidy and consistent and pollution-free excellent seedlings are cultured through careful management and culture.
The embodiment of the invention also discloses an intelligent seedling breeding method which is realized by adopting the intelligent seedling breeding system and comprises the following steps:
(1) preparing a seed suspension with specific seed density and specific agar concentration; the specific seed density is preferably 150-250 grains/ml. The concentration range of the specific agar is 1-5 g/L.
(2) The seed suspension is put into a closed seed suspension germination accelerating tank 2; controlling the temperature in the germination accelerating tank to be 23-26 ℃, and the oxygen content to be 5-8 mg/L.
(3) Uniformly stirring the seed suspension in the closed seed suspension germination accelerating tank by using a stirrer 3;
(4) carry the seed suspension with 5 ways of seed liquid conveyer pipe with the stirring to the seed liquid box 8 of keeping in, portable seeding probe on the automatic dibbler of seed rain slides along slide rail 9 under drive arrangement's effect, changes portable seeding probe and slides to the seed liquid box 8 top of keeping in, and then moves down under drive arrangement and dip in the seed suspension of getting in the seed liquid box 8 of keeping in, the seed suspension that will stir evenly when "reveal white" in the germination accelerating tank is carried to seed liquid box 8 of keeping in through 5 ways of seed liquid conveyer pipe.
(5) The movable sowing probe moves to the upper part of the tissue culture bottle 11 under the action of the driving device, and the seed suspension is evenly dibbled on the culture medium in the tissue culture bottle 11 in a seed rain mode. The seed rain mode is to control the density of liquid drops to be 0.15-0.25 drops/cm 2 and the quantity of each seed drop to be 2-3 seeds.
And (6) conveying the tissue culture bottle 11 with the sown seeds to a bottle covering machine through a conveying belt, covering the bottle cover and placing the bottle cover on a culture rack with a proper light source.
The following description further illustrates the process and beneficial effects of the intelligent seedling breeding method according to the embodiment, with respect to a specific application embodiment of seeding and cultivating dendrobe fruit pods. The method specifically comprises the following steps:
1) the preferable selection of the dendrobium fruit pods: selecting Dendrobium huoshanense fruit pods with relatively consistent individual size and maturity (aiming at ensuring relatively consistent seed quantity, germination vigor and germination rate of Dendrobium huoshanense), and performing shell alcohol sterilization to break the pods for preparing seed suspension.
2) Determination of the seed suspension concentration: according to the germination rate and the sowing density of the dendrobium huoshanense seeds, the number of the seeds in the seed liquid per unit volume is determined to be 150-250 seeds/mL.
2) And (3) blending uniformity of the seed suspension: according to the weight and the suspension force of the dendrobium huoshanense seeds, a method of increasing the concentration (1-3 g/L) of agar in the solution to improve the suspension force and fully stirring is adopted to obtain a uniform dendrobium huoshanense seed suspension.
3) Putting the prepared seed suspension into a translucent closed germination accelerating tank which is wiped clean in advance, and uniformly stirring at the temperature of 23-26 ℃ and the oxygen content of 5-8 mg/L;
4) seeds exposed to the white in the germination accelerating tank (7-10 days) are separately planted on a culture medium in a tissue culture bottle through an intelligent seed rain dibbler, the density of liquid drops is 0.15-0.25 drops/cm 2, and the number of the seeds per drop is 2-3;
5) conveying the tissue culture bottles after the seeds are sown to a bottle covering machine through a conveying belt, covering the bottle covers and placing the bottle covers on a culture rack with a proper light source;
6) the illumination intensity in the whole growth period is maintained at 50-70 mu mol m < -2 > s < -1 >, the illumination time is 8-16 hours, the ambient temperature is 22-26 ℃, and the ambient humidity is 45-65%;
7) and carefully culturing for 180-210 days to obtain the high-quality dendrobium huoshanense seedlings.
The embodiment of the method adopts a traditional Chinese medicine seed suspension liquid culture germination accelerating technology, selects seeds with basically consistent sizes, germination vigor and germination rate, adopts a closed semi-transparent seed liquid germination accelerating tank, realizes the neat and consistent germination of the seeds through the control of temperature and a dissolved oxygen system, and adjusts the buoyancy of the solution through adjusting the concentration of agar in the solution, thereby obtaining the uniformly distributed seed suspension liquid;
the invention adopts an intelligent seed rain dibbling technology, in the invention, seed liquid is dibbled to a culture medium in a tissue culture bottle through a movable seed rain dibbling disk, the volume of each liquid drop is determined according to the seed concentration of a suspension and the seed quantity requirement in each liquid drop, and the number of the seeds dibbled to each tissue culture bottle can be ensured to be basically consistent;
the invention breaks through the traditional culture method of 3 sections (germination, proliferation and rooting) of the tissue culture seedlings of the traditional Chinese medicinal materials, combines the proliferation stage and the rooting stage for culture, reduces the working links, shortens the culture period and saves the production cost.
Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes using the contents of the present specification and drawings, or any other related technical fields, which are directly or indirectly applied thereto, are included in the scope of the present invention.

Claims (11)

1. An intelligent seedling breeding method is realized by adopting an intelligent seedling breeding system, and is characterized in that: the system comprises a germination accelerating tank, a seed liquid conveying pipeline, an automatic seed rain dibbler and a tissue culture bottle, wherein the automatic seed rain dibbler comprises a machine body, a slide rail is arranged on the machine body, a movable sowing probe is arranged on the slide rail in a sliding manner, the movable sowing probe slides along the rail, a seed liquid temporary storage box is further arranged in the machine body, the tissue culture bottle is positioned below the slide rail and the sowing probe, the germination accelerating tank is communicated with the seed liquid temporary storage box through the seed liquid conveying pipeline, the germination accelerating tank is a closed type seed suspension liquid germination accelerating tank, the top height of the seed liquid temporary storage box is lower than that of the germination accelerating tank, the seed liquid conveying pipeline extends out of the top of the germination accelerating tank, extends to the top of the seed liquid temporary storage box along the horizontal direction and is downwards inserted into the seed liquid temporary storage box, the movable sowing probe moves up and down relative to the slide rail, the movable sowing probe slides to the top of the tissue culture bottle from the top of the seed liquid temporary storage box on the track, the movable seeding probe is dipped with the seed liquid in the seed liquid temporary storage box and dibbles the seed liquid into the tissue culture bottle, and the method comprises the following steps:
(1) preparing a seed suspension with specific seed density and specific agar concentration;
(2) loading the seed suspension into a closed seed suspension germination accelerating tank;
(3) uniformly stirring the seed suspension in the closed seed suspension germination accelerating tank by using a stirrer;
(4) the seed suspension liquid which is evenly stirred is conveyed to a seed liquid temporary storage box by a seed liquid conveying pipeline, a movable seeding probe on the seed rain automatic dibbler slides along a slide rail under the action of a driving device, the movable seeding probe slides to the upper part of the seed liquid temporary storage box, and then the movable seeding probe moves downwards under the action of the driving device to dip the seed suspension liquid in the seed liquid temporary storage box,
(5) the movable seeding probe moves to the upper part of the tissue culture bottle under the action of the driving device, the seed suspension is evenly dibbled to the culture medium in the tissue culture bottle in a seed rain mode, and the seed rain mode is control liquidThe drop density is 0.15-0.25 drops/cm2And sowing seeds in a mode that the number of seeds per drop is 2-3.
2. The intelligent seedling breeding method as claimed in claim 1, further comprising the step (6) of conveying the tissue culture bottles with the seeds sowed to a bottle covering machine through a conveyor belt, covering the bottle covers and placing the bottle covers on a culture shelf with a proper light source.
3. The intelligent seedling breeding method as claimed in claim 1, wherein the specific seed density is in the range of 100-3000 seeds/mL.
4. The intelligent seedling breeding method as claimed in claim 1, wherein the specific agar concentration is in the range of 1-10 g/L.
5. The intelligent seedling breeding method according to claim 1, wherein in the step (2), the temperature in the germination accelerating tank is controlled to be 23-26 ℃, and the oxygen content is controlled to be 5-8 mg/l.
6. The intelligent seedling breeding method as claimed in claim 1, wherein in the step (4), when the seeds exposed to white in the germination accelerating tank are transferred to the seed liquid temporary storage box through the seed liquid transfer pipeline, the seed suspension liquid is transferred to the seed liquid temporary storage box.
7. The intelligent seedling breeding method according to claim 1, wherein: the intelligent seedling breeding system comprises a first driving device and a second driving device, wherein the first driving device is arranged on the machine body and used for driving the movable seeding probe to slide along the length direction of the sliding rail, and the second driving device is arranged on the machine body and used for driving the movable seeding probe to move up and down relative to the sliding rail.
8. The intelligent seedling breeding method according to claim 1, wherein: the intelligent seedling breeding system further comprises a conveying belt, a bottle cap machine and a tissue culture basket, wherein the conveying belt is arranged below the track and the movable seeding probe and used for conveying tissue culture bottles, the bottle cap machine is arranged at the tail end of the conveying belt in the conveying direction, the bottle cap machine is used for conveying the tissue culture bottles to the upper cover of the tissue culture bottles when the conveying belts convey the tissue culture bottles to the tail end, the tissue culture bottles are arranged on the tissue culture basket, the bottom of the tissue culture basket is provided with a friction layer for enhancing the friction force of the conveying belts, and a limiting mechanism used for limiting the position of the tissue culture bottles is arranged in the tissue culture basket.
9. The intelligent seedling breeding method according to claim 1, wherein: the germination accelerating tank is communicated with the oxygen adding device through an oxygen conveying pipeline.
10. The intelligent seedling breeding method according to claim 1, wherein: the intelligent seedling breeding system further comprises a temperature control device, wherein the temperature control device is arranged at the bottom of the pregermination tank and used for adjusting the temperature of the planting suspension.
11. The intelligent seedling breeding method according to claim 1, wherein: the seed rain automatic dibbler also comprises a seed liquid volume quantitative setting controller, so as to control the volume of the liquid drops dibbled onto the culture medium by the movable sowing probe according to the concentration of the seed suspension and the seed number demand in the liquid drops.
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