CN203754728U - Cell culture plate for cell immunofluorescence and immunochemical research - Google Patents
Cell culture plate for cell immunofluorescence and immunochemical research Download PDFInfo
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- CN203754728U CN203754728U CN201420110956.2U CN201420110956U CN203754728U CN 203754728 U CN203754728 U CN 203754728U CN 201420110956 U CN201420110956 U CN 201420110956U CN 203754728 U CN203754728 U CN 203754728U
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Abstract
The utility model discloses a cell culture plate for cell immunofluorescence and immunochemical research. The height of a plate body accounts for 1/2-2/3 of that of a merchant common culture plate; the bottom surface of the plate body is a horizontal plane, and a division wall which is protruded upwards slightly is arranged on the bottom surface; the division wall is used for dividing the bottom surface of the culture plate body into N*(N+1) rectangular chambers, wherein N is equal to 2-6, and the height of the division wall accounts for 1/4 of that of the plate body; a concave right angle for marking the direction of the plate body is arranged in one of the corners of the plate body. By adopting the cell culture plate disclosed by the utility model, cells can be uniformly dispersed into each square chamber, and can growth in the same culture environment so as to avoid experimental differences, and meanwhile, differential treatment (for example, adding different antibodies or reagents) and same treatment (such as cell fixation, sealing, washing and the like) performed on the cells in the different square chambers can be realized at the same time; the experiment efficiency can be improved, the cost can be reduced, and the comparability of experiment results can be achieved.
Description
Technical field
The utility model relates to a kind of Tissue Culture Plate, relates in particular to a kind of Tissue Culture Plate that is applicable to carry out cellular immunofluorescence and immunocytochemistry research; Belong to biology and medical field experimental apparatus.
Background technology
Carrying out in modern biology and medical research and clinical detection, cellular immunofluorescence and immunocytochemistry experiment are a kind of conventional technique means.The method adopting is at present: by cell cultures in 96 well culture plates (or being positioned on the cover glass in culture dish), treat that cell attachment grows and grow to logarithmic growth after date, discard nutrient solution, to its be fixed, the experimental implementation such as sealing, primary antibodie are hatched, clean, two anti-ly hatch, clean, colour developing or Fluirescence observation.But the method exists every hole (or on every cover glass) cell and needs operation separately, the problem such as existence condition discordance in treating processes, and the expense reagent of wasting time and energy, be prone to error; In addition sometimes because of the restriction of micro objective operating distance also there will be cell observation in 96 well culture plates less than or unclear problem.
Summary of the invention
For problems of the prior art and deficiency, the technical problems to be solved in the utility model is to provide a kind of Tissue Culture Plate that is applicable to carry out cellular immunofluorescence and immunocytochemistry research.
The top cover that the Tissue Culture Plate that is applicable to carry out cellular immunofluorescence and immunocytochemistry research described in the utility model mates by plate body with plate body forms; It is characterized in that: the height of described plate body is 1/2～2/3 of commercially available common culture plate height; The bottom surface of described plate body is horizontal plane, and is provided with the partition wall that upwards omits micro-protuberance in bottom surface, is separated into N × (N+1) individual rectangle cell cultivating plate body bottom surface, wherein N=2～6, and the height of partition wall is 1/4 of plate body height; Wherein one jiao of spill right angle that is provided with the plate body direction that serves as a mark of described plate body.
Laboratory can be according to demand, selects the product of above-mentioned different size.Wherein, preferred embodiment: N=2～3, the thickness of described partition wall is 0.2～0.4 millimeter, the length of side of square chamber is 5～8 millimeters.
Further, above-mentioned plate body take spill right angle as mark initial point, is carved with the label symbol of identification cell in the side of the both direction of plate body.
Cultured cells is inoculated in after Tissue Culture Plate described in the utility model, cell can be scattered in each rectangle cell uniformly, and in plate body bottom, cultured cells is separated, does not separate on the top that the height of nutrient solution is plate body higher than the height of partition wall, cell is grown in identical culture environment, both handled easily, has avoided again the difference of experiment condition and result.Wherein, the height of plate body is more convenient for using various types of microscopes to observe lower than the height of conventional culture plate.
The Tissue Culture Plate that what the utility model provided be applicable to carries out cellular immunofluorescence and immunocytochemistry research, cell cultures and cellular immunofluorescence/immunocytochemistry are blended in to one, both simplify operating process, strengthened again the consistence of experimentation.Compare with existing culture plate, the bottom design of Tissue Culture Plate of the present utility model has upwards the separation thin-walled of projection slightly, in plate body bottom, cultured cells is separated, and is convenient to the later stage cell is carried out to differential operation; Does not separate on the top of plate body, is convenient to carry out cell cultures and later stage to carry out the operation of other steps in cellular immunofluorescence and immunocytochemistry research.Time saving and energy saving, both simplified operation, improve again the consistence of experimentation.
Brief description of the drawings
Fig. 1: be the schematic side view of the utility model culture plate structure.
Fig. 2: be the schematic top plan view of the utility model culture plate.
Fig. 3: be the concrete enforcement schematic diagram of the utility model culture plate.
Wherein: label symbol, the spill right angle of 48 mark plate body directions, 49 adherent growth cell on culture plate bottom surface of signal, 45 rectangle cells, 46 partition walls, 47 cells do not separated on the top of 41 top covers, 42 plate bodys, 43 plate bodys.
Embodiment
Embodiment 1
Shown in Fig. 1～3, and the culture plate that preferably contains 2 × 3 square chamber taking Fig. 3 the utility model is as example, and concrete structure, embodiment and advantage are described in further detail.
As depicted in figs. 1 and 2, the top cover 41 that the Tissue Culture Plate that is applicable to carry out cellular immunofluorescence and immunocytochemistry research described in the utility model mates by plate body 42 with plate body forms; Wherein: the height of described plate body is 2/3 of commercially available common culture plate height; The bottom surface of described plate body is horizontal plane, and be provided with the partition wall 46 that upwards omits micro-protuberance in bottom surface, be separated into the square chamber 45 of 12 × 3 cultivating plate body bottom surface, the height of partition wall 46 is 1/4 of plate body height, the thickness of partition wall is 0.4 millimeter, and the length of side of square chamber is 6 millimeters; Described plate body 42 wherein one jiao be provided with the spill right angle 48 of the plate body direction that serves as a mark, and take spill right angle as mark initial point, be carved with the label symbol 47 of identification cell in the side of the both direction of plate body.
Cell to be studied is mixed with to suitable concentration with nutrient solution, after mixing, joins in above-mentioned culture plate, make liquid level mediate 51, like this, although cell is dispersed in different cells, cell is all grown in identical liquid environment.Culture plate is positioned in incubator and is cultivated after for some time, and cell attachment is grown on culture plate bottom surface 49.
In the time will carrying out cellular immunofluorescence and immunocytochemistry experiment, culture plate is taken out, suck nutrient solution, add physiological saline or PBS in culture plate, to clean together the cell in each hole, follow-up step can be carried out co-treatment as fixing, saturatingization, sealing, colour developing, cleaning etc., has avoided the drawback by hole operation with common culture plate; In the time that needs carry out differential processing to cell, can be the each square chamber of label symbol 47 mark that identification cell is carved with in spill right angle 48 or plate body side according to the right angle concave shaped design of 42 1 jiaos of plate bodys, the particular solution (as primary antibodie, two anti-etc.) that adds experimental amount according to experimental design in corresponding square chamber, whole experimentation can not produce the situation that between each cell, solution mixes.And time saving and energy saving, both simplified operation, improve again the consistence of experimentation.
As mentioned above, can be according to experiment real needs, select to prepare the cell cultures panel products that is applicable to carry out cellular immunofluorescence and immunocytochemistry research described in the utility model of different size.As, the slightly partition wall 46 of micro-protuberance is set upwards cultivating plate body bottom surface, cultivation plate body bottom surface is separated into N × (N+1) individual rectangle cell (45), and N=2～6 and arranges arbitrarily, can obtain the product described in the similar embodiment 1 of more specifications.
Embodiment 2
Need to carry out cellular immunofluorescence and immunocytochemistry experiment according to experiment, select applicable microscope to observe.
The height of plate body is 1/2 of commercially available common culture plate height; The height of partition wall 46 is 1/4 of plate body height, and the top 3/4 of plate body does not separate 43, at the bottom of can being convenient to object lens Close approach plate, has both been applicable to, with the cell at the bottom of inverted microscope access panel, being applicable to again with the cell at the bottom of conventional microscopic examination plate.
Claims (3)
1. for the Tissue Culture Plate of cellular immunofluorescence and immunochemistry research, formed by plate body (42) and the top cover (41) that mates with plate body; It is characterized in that: the height of described plate body (42) is 1/2～2/3 of commercially available common culture plate height; The bottom surface of described plate body (42) is horizontal plane, and be provided with the partition wall (46) that upwards omits micro-protuberance in bottom surface, be separated into N × (N+1) individual rectangle cell (45) cultivating plate body bottom surface, wherein N=2～6, the height of partition wall (46) is 1/4 of plate body (42) height; Wherein one jiao of spill right angle (48) that is provided with the plate body direction that serves as a mark of described plate body (42).
2. the Tissue Culture Plate for cellular immunofluorescence and immunochemistry research according to claim 1, it is characterized in that: described N=2～3, the thickness of described partition wall (46) is 0.2～0.4 millimeter, and the length of side of square chamber (45) is 5～8 millimeters.
3. the Tissue Culture Plate for cellular immunofluorescence and immunochemistry research according to claim 1, it is characterized in that: described plate body (42), taking spill right angle (48) as mark initial point, is carved with the label symbol (47) of identification cell in the side of the both direction of plate body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420110956.2U CN203754728U (en) | 2014-03-12 | 2014-03-12 | Cell culture plate for cell immunofluorescence and immunochemical research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420110956.2U CN203754728U (en) | 2014-03-12 | 2014-03-12 | Cell culture plate for cell immunofluorescence and immunochemical research |
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| Publication Number | Publication Date |
|---|---|
| CN203754728U true CN203754728U (en) | 2014-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420110956.2U Expired - Fee Related CN203754728U (en) | 2014-03-12 | 2014-03-12 | Cell culture plate for cell immunofluorescence and immunochemical research |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251859A (en) * | 2018-10-11 | 2019-01-22 | 广州洁特生物过滤股份有限公司 | A kind of cell culture apparatus and its cell box |
-
2014
- 2014-03-12 CN CN201420110956.2U patent/CN203754728U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251859A (en) * | 2018-10-11 | 2019-01-22 | 广州洁特生物过滤股份有限公司 | A kind of cell culture apparatus and its cell box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140806 Termination date: 20160312 |