CN111471592A - Cell culture bottle and manufacturing method thereof - Google Patents

Abstract

A cell culture bottle comprises a bottle body, one end of the bottle body is provided with a bottle cap, a side wall of the bottle body is provided with a slot inlet, the slot inlet is embedded with a connector, and the connector is embedded with the side of the slot inlet connected together and one end protrudes from the outer end of the bottle; the bottom of the connector is provided with a test paper holder, the test paper holder is perpendicular to the connector, the top is embedded in the connector and fixed, and the bottom is suspended in the bottle; the test paper holder Set the test strip slot. A method for manufacturing a cell culture flask, comprising the following steps: 1) prefabricating parts; 2) assembling parts. The invention can solve the technical problem that the existing cell culture flask cannot detect the contamination of cells in real time.

Description

A cell culture flask and method of making the same

technical field

The invention belongs to the technical field of scientific instruments, in particular to a cell culture flask and a manufacturing method thereof.

Background technique

Cell culture refers to a method that simulates the in vivo environment in vitro, allowing it to grow, multiply and maintain its primary structure and function. Cell culture, also known as cell cloning technology, is an indispensable technology in modern medicine and cell biology research methods. Microbial contamination is a relatively common event during cell culture. Currently, bacterial, fungal, and mycoplasma contamination are the most common microbial contamination during cell culture. If the cells are contaminated by microorganisms, it will not only affect the growth state of the cells and the experimental results, but also lead to a series of serious consequences such as waste of time and scientific research funds and the inability to repeat the experiments. Therefore, detecting the contamination of the cell culture medium and finding and determining the type of microbial contamination in time to remove the contamination is one of the keys to successfully culturing cells and the basis for a successful experiment. However, the current methods for detecting cell culture media contamination (culture method, fluorescent staining method, ELISA method, etc.) are relatively complicated, and it is difficult to detect and determine the type of contaminating microorganisms in the early stage of cell contamination, thus affecting the actual progress of the experiment.

The "cell culture flask" provided by the patent application number "CN200720048543.6" only has the accommodation conditions for cell culture, but does not have the detection conditions.

SUMMARY OF THE INVENTION

In order to overcome the above-mentioned defects of the prior art, the present invention provides a cell culture flask and a manufacturing method thereof, which can solve the technical problem that the existing cell culture flask cannot detect the contamination of cells in real time.

The present invention adopts following technical scheme:

A cell culture bottle comprises a bottle body, one end of the bottle body is provided with a bottle cap, a side wall of the bottle body is provided with a slot inlet, the slot inlet is embedded with a connecting piece, and the connecting piece is embedded with the side of the slot inlet connected together and one end protrudes from the outer end of the bottle; the bottom of the connector is provided with a test paper holder, the test paper holder is perpendicular to the connector, the top is embedded in the connector and fixed, and the bottom is suspended in the bottle; the test paper holder Set the test strip slot.

Preferably, the test paper basket includes a bottom plate and a back plate, a pair of side plates are arranged on both sides of the bottom plate, a plurality of blocking bars are connected between a pair of the side plates, and the space between the blocking bars and the back plate For the test paper slot.

Preferably, a plurality of baffles parallel to the side plates are arranged on the back panel toward the baffle bars to separate the test paper slot into a plurality of test paper accommodating frames, and the test paper accommodating frames are placed in the test paper accommodating frames.

Preferably, there are two baffles, and the test paper slot is divided into three test paper accommodating frames, and the test papers in the test paper accommodating frames are bacteria detection test paper, fungus detection test paper and mycoplasma test paper respectively.

Preferably, the bottle body is square, and one side of the bottle is connected to the bottle cap through a bevel neck, and the bevel neck is threadedly connected to the bottle cap.

Preferably, each side wall of the card slot inlet is provided with a convex rib protruding from the side wall, and a corresponding groove is provided on the connecting piece and the convex rib of the side wall of the card slot inlet, and the convex rib One is embedded in the groove and one is tightly fitted.

Preferably, two convex ribs protruding from both sides of the back plate are provided on both sides of the top of the back plate, and the bottom of the connecting piece is provided with grooves corresponding to the top of the back plate, and two grooves are provided corresponding to the convex ribs, and the back plate is provided with two grooves. The top is embedded in the groove, and the two convex ribs are embedded in the groove for tight fit.

Preferably, the material of the connector is rubber.

A method for making a cell culture flask, comprising the following steps:

1) Prefabricated parts: Prefabricate the bottle body and neck, bottle cap, and test paper basket through an injection molding machine; cut the rubber raw material into a connector;

2) Assembling the parts: After inserting the top of the back plate into the groove of the connector, and inserting the second ridge into the second groove to fit and connect, put the test paper into the test paper accommodating frame, and remove the test paper holder from the card slot. The inlet is placed in the bottle body, the two sides of the connector are inserted into the inlet of the slot, and the first ridge and the first groove are fitted into each other.

The present invention has the following positive beneficial effects:

Using the culture flask of the present invention can detect suspicious contamination in time, and determine the corresponding contaminating microorganism type, therefore, the time required for determining whether the cells are contaminated and the contamination type can be greatly reduced, and corresponding measures can be taken in time to reduce the loss to lowest.

The culture bottle used in the present invention can be especially applied to a square oblique bottle, and has the advantages of a square oblique bottle: the arrangement of the connecting piece does not affect the flatness of the top of the bottle body, so it can be stacked and placed to save space; cultivation area.

Description of drawings

Fig. 1 is a perspective view of a culture flask of the present invention.

FIG. 2 is a plan view of FIG. 1 .

FIG. 3 is a structural diagram of FIG. 2 with the rubber sheet removed.

FIG. 4 is a cross-sectional view of the bottle body A-A in FIG. 2 .

FIG. 5 is a front view of the test paper basket with test paper.

FIG. 6 is a cross-sectional view of the test paper basket at the position B-B in FIG. 4 .

7 is a schematic diagram of the overall structure of the present invention without the top of the bottle body.

Number: 1-bottle body, 11-card slot entrance, 111-rib one, 2-bottle cap, 3-connector, 31-groove one, 32-groove, 33-groove two, 4-test paper holder Basket, 41- bottom plate, 42- back plate, 421- rib, 43- side plate, 44- blocking lever, 45- baffle plate, 46- test paper accommodating frame, 5- test paper.

Detailed ways

The specific embodiments of the present invention will be further described below with reference to the accompanying drawings.

The following examples are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those of ordinary skill in the art, on the basis of the following description, other changes or changes in different forms can also be made, and these obvious changes or changes which belong to the spirit of the present invention are still within the protection scope of the present invention. middle.

1-7, a cell culture bottle includes a bottle body 1, a bottle cap 2 is provided at one end of the bottle body 1, a slot inlet 11 is provided on the side wall of the bottle body 1, and the slot inlet 11 is embedded in a connector 3. The connector 3 is fitted and connected with the side wall of the slot inlet 11 and one end extends out of the outer end face of the bottle body 1; , the top is embedded in the connector 3 and fixed, and the bottom is suspended in the bottle body 1; the test paper tray 4 is provided with a test paper slot.

Further, referring to FIGS. 3-7 , the test paper basket 4 includes a bottom plate 41 and a back plate 42, a pair of side plates 43 are provided on both sides of the bottom plate 41, and a plurality of blocking bars are connected between a pair of the side plates 43 44. The space between the blocking rod 44 and the back plate 42 is a test paper slot.

Further, referring to FIGS. 4 and 5 , a plurality of baffles 45 parallel to the side plates 43 are arranged on the back plate 42 toward the blocking bar 44 to separate the test paper slot into a plurality of test paper accommodating frames 46 , and the test paper accommodating frames A test strip 47 is placed inside 46 .

Further, referring to FIG. 6 , there are two baffles 45 , and the test paper slot is divided into three test paper accommodating frames 46 , and the test papers 5 in the test paper accommodating frames 46 are respectively bacteria detection test paper and fungus detection test paper. Test strips and Mycoplasma test strips.

Further, referring to FIG. 1 , the bottle body 1 is a square shape, and one side of the bottle body 1 is connected to the bottle cap 2 through a bevel neck, and the bevel neck is threadedly connected to the bottle cap 2 .

Further, referring to FIG. 4 , each side wall of the card slot inlet 11 is provided with a rib one 111 protruding from the side wall, and the connector 3 is provided corresponding to the rib one 111 of the side wall of the card slot inlet 11 The groove one 31, the convex edge one 111 is embedded in the groove one 31 for a tight fit.

Further, two convex ribs 421 protruding from both sides of the back plate 42 are provided on both sides of the top of the back plate, the bottom of the connecting member 3 is provided with a groove 32 corresponding to the top of the back plate 42, and a groove is provided corresponding to the two convex ribs 421. Second 33, the top of the back plate 42 is inserted into the groove 32, and the second convex rib 421 is inserted into the second groove 33 for a tight fit.

Further, the material of the connecting piece 3 is rubber.

A method for making a cell culture flask, comprising the following steps:

1) Prefabricated parts: Prefabricate the bottle body 1 and the bottle neck, bottle cap 2, test paper basket 4 through the injection molding machine; cut the rubber raw material into the connector 3;

2) Assembling the parts: after inserting the top of the back plate 42 into the groove 32 of the connector 3, and inserting the second rib 421 into the second groove 33 for fitting and connection, put the test paper 5 into the test paper accommodating frame 46, Put the test paper basket 4 into the bottle body 1 from the slot inlet 11, plug both sides of the connector 3 into the slot inlet 11, and fit the ridge one 111 and the groove one 33 to each other.

On the basis of the original culture bottle, the invention patent can detect the cell contamination in real time while the cell is cultured through a test paper basket 4 that can accommodate the cell contamination detection test paper, so as to timely discover and determine the species of contaminating microorganisms, and then take early treatment. and countermeasures to avoid unnecessary consequences.

The test paper of the invention can detect common types of microbial contamination (bacteria, fungi, mycoplasma, etc.), and if contamination of bacteria, fungi and mycoplasma occurs during the cell culture process, the test paper can detect in real time whether there is contamination and the types of microorganisms that are polluted. During the cultivation process, if the above-mentioned common contaminating microorganism types appear, the color of the corresponding test paper will change. By observing the change of the test paper, it can be judged whether there is common microorganism contamination in the cells, and corresponding treatment measures can be guided.

The principle of the test paper for detecting bacteria is based on the antigen-antibody combined immunological detection method of peptidoglycan, a component of bacterial cell wall, and is realized in the form of test paper. The principle of the test paper for detecting fungi is based on the antigen-antibody immunological detection method of the fungal cell wall component chitin and is realized in the form of test paper. The principle of the test paper for the detection of mycoplasma is based on the antigen-antibody immunological detection method for the mycoplasma itself and is realized in the form of test paper. The realization form of immunological test paper and the preparation of peptidoglycan, chitin and mycoplasma antibodies are all in the prior art, and the present invention only utilizes the test paper prepared by using the prior art, so its principle and preparation method will not be repeated.

During cell culture, when cells and culture medium are in culture flasks, microbial contamination test strips come into contact with the culture medium. If common bacterial, fungal or mycoplasma infections are present in the culture medium during the culture process, these strips can be sensitively detected. Corresponding microorganisms are generated to respond to the observer, therefore, the present invention can quickly and conveniently detect whether there is common microorganism contamination in the cell culture process.

Claims (9)

1. A cell culture bottle comprises a bottle body (1), wherein one end of the bottle body (1) is provided with a bottle cap (2), and is characterized in that the side wall of the bottle body (1) is provided with a clamping groove inlet (11), the clamping groove inlet (11) is embedded into a connecting piece (3), the connecting piece (3) is connected with the side wall of the clamping groove inlet (11) in an embedded manner, and one end of the connecting piece extends out of the outer end face of the bottle body (1); the bottom of the connecting piece (3) is provided with a test paper basket (4), the test paper basket (4) is vertical to the connecting piece (3), the top of the test paper basket is embedded into the connecting piece (3) for fixing, and the bottom of the test paper basket is suspended in the bottle body (1); a test paper groove is arranged in the test paper holding basket (4).

2. The cell culture bottle of claim 1, wherein the test paper basket (4) comprises a bottom plate (41) and a back plate (42), a pair of side plates (43) are arranged on two sides of the bottom plate (41), a plurality of blocking rods (44) are connected between the pair of side plates (43), and a space between the blocking rods (44) and the back plate (42) is a test paper slot.

3. The cell culture bottle according to claim 2, wherein the back plate (42) is provided with a plurality of baffles (45) parallel to the side plates (43) and facing the stop rods (44) to divide the test strip slot into a plurality of test strip accommodating frames (46), and the test strips (47) are arranged in the test strip accommodating frames (46).

4. A cell culture flask according to claim 3, wherein the number of the baffles (45) is two, the test strip slot is divided into three test strip accommodating frames (46), and the test strips (5) in the test strip accommodating frames (46) are respectively bacteria test strips, fungi test strips and mycoplasma test strips.

5. A cell culture flask according to claim 1, wherein the flask body (1) is square and is connected to the flask cap (2) at one side by a neck, said neck being screwed to the flask cap (2).

6. The cell culture bottle as claimed in claim 1, wherein each side wall of the inlet (11) is provided with a first rib (111) protruding from the side wall, the connecting piece (3) and the first rib (111) on the side wall of the inlet (11) are provided with a corresponding first groove (31), and the first rib (111) is inserted into the first groove (31) for tight fit.

7. The cell culture bottle as claimed in claim 2, wherein two ribs (421) protruding from two sides of the top of the back plate (42) are provided on two sides of the top of the back plate, the bottom of the connector (3) is provided with a groove (32) corresponding to the top of the back plate (42) and a groove (33) corresponding to the rib (421), the top of the back plate (42) is inserted into the groove (32), and the rib (421) is inserted into the groove (33) with tight fit.

8. A cell culture flask according to claim 1, wherein the connecting element (3) is made of rubber.

9. A method of making a cell culture flask according to claims 1-8, comprising the steps of:

1) prefabricating parts: prefabricating a bottle body (1), a bottle neck, a bottle cap (2) and a test paper basket (4) through an injection molding machine; cutting a rubber raw material into a connecting piece (3);

2) assembling parts: the top of the back plate (42) is plugged into the groove (32) of the connecting piece (3), and the second rib (421) is plugged into the second groove (33) to be embedded and connected, the test paper is placed into the test paper accommodating frame (46) and the test paper supporting basket (4) is placed into the bottle body (1) from the clamping groove inlet (11), and the two sides of the connecting piece (3) are plugged into the clamping groove inlet (11) and the first rib (111) and the first groove (33) are embedded with each other.

Images