CN207294805U - A kind of cell co-cultures bottle - Google Patents

Abstract

The utility model discloses a kind of cell to co-culture bottle,Including cultivating bottle,The culture bottle includes two culture bottlenecks of two co-walled rectangle culturing room and connection culturing room,The co-walled inner wall surface of two culturing room is equipped with opening,The opening, which is equipped with, passes through film,Two culture bottlenecks are connected with ventilating cover,The ventilating cover is equipped with the stomata of multiple annular arrays,The HDPE film and dPTFE films of covering stomata are equipped with ventilating cover,Common two, blake bottle is combined into one to the utility model on the market,It is co-walled among two bottles,Add the capacity of cell culture chamber,The cell of Liang Ge culturing room can be carried out at the same time individually operated,Design through film can Shi Liangge culturing room the iuntercellular factor realize it is common,And two kinds of cells cannot pass through,Suitable for attached cell,The foundation of the co-culture system of suspension cell and half suspension cell,It is operationally more easy to operate,Will not mutual contamination of cells,And it may be reused,Reduce cost.

Description

A kind of cell co-cultures bottle

Technical field

It the utility model is related to cell culture apparatus field, and in particular to a kind of cell co-cultures bottle.

Background technology

The interaction of cell and microorganism is that living nature generally occurs, the co-cultivation room research of cell or microorganism The basic means of cell and microbial interaction, the scientific research of early stage is using single cell or single microorganism as grinding Study carefully, the research to interact between cell is less, and the research of cell and microbial interaction is also less, with the depth of investigation Increase, these interactions actually belong to the information interchange between a kind of intercellular information interchange, microorganism and cell Exchanging between microorganism.This information interchange has a great impact microorganism and cell, affect they propagation, Differentiation, material secretion.

Since the search time of interaction is longer, the research of direct interaction is relatively difficult.It is because different thin Born of the same parents are mixed, and different cell growth cycles are different, and a kind of cell is likely to grow the existence of another cell of too fast contention Space, is not easy to observe；What is more important mixed culture cell is inconvenient to be distinguished, and causes cell in-situ identification to there is certain be stranded Difficulty, and iuntercellular or cell and the Indirect Interaction of microorganism are then conducive to the information interchange of scientific research personnel's discovery therebetween, It is found that many important phenomenons, to prevent and cure diseases, controls the biology shape of cell and microorganism to provide strategy.

Utility model content

For in the prior art the defects of, the purpose of this utility model, which is to provide, a kind of to be had large capacity, can be mixed And the cell of independent operation co-cultures bottle.

The technical solution adopted in the utility model is that a kind of cell co-cultures bottle, including culture bottle, the culture bottle Two culture bottlenecks including two co-walled rectangle culturing room and connection culturing room, the co-walled inner wall surface of two culturing room are equipped with Opening, the opening, which is equipped with, passes through film, and two culture bottlenecks are connected with ventilating cover, and the ventilating cover is equipped with the gas of multiple annular arrays Hole, the HDPE film and dPTFE films of covering stomata are equipped with ventilating cover, for by carbon dioxide and playing waterproof action.

In the technical program, Liang Ge culturing room shares a side wall, adds the capacity of cell culture chamber, while can be to two The cell progress of a culturing room is individually operated, and co-walled side wall is equipped with opening, and opening, which is equipped with, passes through film, and design can make through film The iuntercellular factor of Liang Ge culturing room is realized common, and two kinds of cells cannot be by suitable for attached cell, suspension cell and half The foundation of the co-culture system of suspension cell, it is operationally more easy to operate, will not mutual contamination of cells, and can repeat to make With reducing cost.

Further, the two cultures bottleneck is inclined upwardly, to prevent nutrient solution from outflowing.

In such manner, it is possible to liquid avoids culturing room from being subject to outside contamination while avoiding cultivating indoor nutrient solution outflow.

Further, the filter opening aperture through film is less than 40 microns.

In such manner, it is possible to the Shi Liang culturing room iuntercellular factor is realized common while avoids two kinds of mixing with cells.

Further, the opening is square openings that the length of side is 0.5 centimetre, extended distance culture bottle bottom surface 0.2 to 0.3 centimetre.

In this way, certain separate space is left, avoids pancreatin from passing through through film during digestion easy to attached cell And influence other cells.

Further, it is polystyrene to cultivate bottle material.

In this way, having high transparency using the blake bottle of polystyrene material, light transmittance is beneficial to up to more than 90% Cell culture state is observed under the microscope, and in addition also there is good rigidity and chemical resistance.

Further, the film that passes through is polycarbonate membrane.

The beneficial effects of the utility model are：Two common on the market, blake bottle is combined into one, among two bottles It is co-walled, the capacity of cell culture chamber is added, while can be individually operated to the progress of the cell of Liang Ge culturing room, design can through film The iuntercellular factor of Shi Liangge culturing room is realized common, and two kinds of cells cannot be by, suitable for attached cell, suspension cell and The foundation of the co-culture system of half suspension cell, it is operationally more easy to operate, will not mutual contamination of cells, and can repeat Use, reduce cost.

Brief description of the drawings

, below will be right in order to illustrate more clearly of the utility model embodiment or technical solution of the prior art Embodiment or attached drawing needed to be used in the description of the prior art are briefly described.In all of the figs, it is similar Element or part are generally identified by similar reference numeral.In attached drawing, each element or part might not be according to actual ratios Draw.

Fig. 1 is the three-dimensional structure diagram of the utility model.

Wherein：1- culturing room, 2- culture bottlenecks, 3- ventilating covers, 301- stomatas, 4- pass through film

Embodiment

The embodiment of technical solutions of the utility model is described in detail below in conjunction with attached drawing.Following embodiments are only For clearly illustrating the technical solution of the utility model, therefore example is only used as, and this practicality cannot be limited with this New protection domain.

When it is implemented, as shown in Figure 1, a kind of cell co-cultures bottle, including culture bottle, the culture bottle includes two A co-walled rectangle culturing room 1 and two culture bottlenecks 2 of connection culturing room 1, the co-walled inner wall surface of two culturing room 1 are equipped with Opening, the opening, which is equipped with, passes through film 4, and two culture bottlenecks 2 are connected with ventilating cover 3, and the ventilating cover 3 is equipped with multiple annular arrays Stomata 301, the HDPE film and dPTFE films of covering stomata are equipped with ventilating cover 3, for by carbon dioxide and playing anti- Water acts on.

In the technical program, Liang Ge culturing room 1 shares a side wall, adds the capacity of cell culture chamber 1, while can be right The cell progress of Liang Ge culturing room 1 is individually operated, and co-walled side wall is equipped with opening, and opening, which is equipped with, passes through film 4, and design passes through film 4 can the iuntercellular factor of Shi Liangge culturing room 1 realize common, and two kinds of cells cannot be by, suitable for attached cell, suspend it is thin The foundation of the co-culture system of born of the same parents and half suspension cell, it is operationally more easy to operate, will not mutual contamination of cells, and can be with Reuse, reduce cost.

As shown in Figure 1, the two cultures bottleneck 2 is inclined upwardly, to prevent nutrient solution from outflowing.In such manner, it is possible to avoid training Liquid avoids culturing room 1 from being subject to outside contamination while supporting the outflow of the nutrient solution in room 1.

As shown in Figure 1, the filter opening aperture through film 4 is less than 40 microns.In such manner, it is possible to 1 iuntercellular of Shi Liang culturing room The factor is realized common while avoids two kinds of mixing with cells.

As shown in Figure 1, the opening is the square openings that the length of side is 0.5 centimetre, extended distance culture bottle bottom surface 0.2-0.3 centimetres.In this way, certain separate space is left, avoids pancreatin from passing through saturating during digestion easy to attached cell Cross film 4 and influence other cells.

As shown in Figure 1, culture bottle material is polystyrene.In this way, there is pole using the blake bottle of polystyrene material High transparency, light transmittance is up to more than 90%, beneficial to cell culture state is observed under the microscope, in addition also has well The advantages that rigidity and chemical resistance.

As shown in Figure 1, the film that passes through is polycarbonate membrane.

In summary：The utility model has the advantages that simple in structure, easy to operate.

Finally it should be noted that：Various embodiments above is only to illustrate the technical solution of the utility model, rather than it is limited System；Although the utility model is described in detail with reference to foregoing embodiments, those of ordinary skill in the art should Understand：It can still modify the technical solution described in foregoing embodiments, either to which part or whole Technical characteristic carries out equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from this practicality newly The scope of each embodiment technical solution of type, it should all cover among the claim of the utility model and the scope of specification.

Claims (6)

1. a kind of cell co-cultures bottle, it is characterised in that：Including cultivating bottle, the culture bottle includes two co-walled rectangles Culturing room and two culture bottlenecks of connection culturing room, the co-walled inner wall surface of two culturing room are equipped with opening, and the opening is equipped with Through film, two culture bottlenecks are connected with ventilating cover, and the ventilating cover is equipped with the stomata of multiple annular arrays, is equipped with and covers in ventilating cover The HDPE film and dPTFE films of lid stomata, for by carbon dioxide and playing waterproof action.

2. a kind of cell according to claim 1 co-cultures bottle, it is characterised in that：The two cultures bottleneck is inclined upwardly, To prevent nutrient solution from outflowing.

3. a kind of cell according to claim 1 co-cultures bottle, it is characterised in that：The filter opening aperture through film is less than 40 microns.

4. a kind of cell according to claim 1 co-cultures bottle, it is characterised in that：The opening is that the length of side is 0.5 centimetre Square openings, 0.2 to 0.3 centimetre of extended distance culture bottle bottom surface.

5. a kind of cell according to claim 1 co-cultures bottle, it is characterised in that：It is polystyrene to cultivate bottle material.

6. a kind of cell according to claim 1 co-cultures bottle, it is characterised in that：The film that passes through is polycarbonate membrane.