CN219807967U - Auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane - Google Patents
Auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane Download PDFInfo
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- CN219807967U CN219807967U CN202321102283.1U CN202321102283U CN219807967U CN 219807967 U CN219807967 U CN 219807967U CN 202321102283 U CN202321102283 U CN 202321102283U CN 219807967 U CN219807967 U CN 219807967U
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- Prior art keywords
- base
- cell culture
- culture plate
- rectangular groove
- monoclonal antibody
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- 210000003617 erythrocyte membrane Anatomy 0.000 title claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims abstract description 26
- 238000004113 cell culture Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 2
- 210000003743 erythrocyte Anatomy 0.000 claims 4
- 239000012528 membrane Substances 0.000 claims 4
- 238000010790 dilution Methods 0.000 abstract description 12
- 239000012895 dilution Substances 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 6
- 238000003113 dilution method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 238000010367 cloning Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an erythrocyte membrane monoclonal antibody subcloning auxiliary structure, which comprises a base and a 96-hole cell culture plate, wherein the upper end surface of the base is provided with a rectangular groove, the 96-hole cell culture plate is arranged in the rectangular groove, and the rectangular groove divides the upper end surface of the base into two parts and forms two identification surfaces; the wells on the 96-well cell culture plate are divided into 12 columns, and the labels for displaying the cell numbers and the cell liquid sucking amounts in the third to seventh rows of the wells in the 12 columns are sequentially arranged on the two identification surfaces. The utility model is convenient to process and use. The 96-hole cell culture plate is placed in the rectangular groove, limiting dilution is carried out by comparing the parameter marks on the left side and the right side of the device through the cell count number, the limiting dilution is more convenient, the workload is lightened, the error caused by limiting dilution is prevented, the number of single groups is reduced, and the subcloning times are increased.
Description
Technical Field
The utility model relates to an auxiliary structure of monoclonal antibody of erythrocyte membrane.
Background
The hybridoma technique is to fuse B lymphocytes that secrete antibodies with myeloma cells that can proliferate indefinitely in vitro, thereby obtaining hybridoma cells that can proliferate and secrete antibodies for a long period in vitro. The technology is widely applied to the preparation of monoclonal antibodies, and the monoclonal antibodies have high specificity, so that the technology is widely applied to the aspects of the diagnosis and treatment of serious diseases, the identification, the positioning, the separation and the like of biological macromolecules. Thus, the application of hybridoma technology is becoming more and more widespread and important.
The fused cells need to be subjected to further screening by cloning after screening, and the cells with high secretion are selected. The cloning methods commonly used at present are as follows: limiting dilution method, semi-solid culture method, micromanipulation method, flow sorting method, etc. The limited dilution method is the simplest and easy to operate, and complex instruments and equipment are not needed, so that the limited dilution method is the most widely applied. The principle is that the cells in the culture holes are blown by a gun or a suction pipe to suspend the cells, then counted by a counting plate, finally the original hole cells are diluted and dripped on a new culture plate according to the amount of 0.5-1 cells per hole, and after a period of culture, part of the holes can be seen to have single clone formation. In practical experimental operation, the cell loss is often caused by inaccurate counting or blowing of a liquid-transfering device, so that the cell number of each hole is properly widened after the cell counting, and the proportion of single clone in the hole is increased. In addition, the cell number obtained by counting needs to be calculated in limited dilution, then the multiple ratio dilution is carried out, and the calculation is carried out after each counting, so that the complicated degree of the experiment and the operation time of the experiment are increased, and the manpower and material resources are wasted.
Disclosure of Invention
The utility model provides an auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane in order to solve the problems existing in the prior art.
The utility model adopts the technical scheme that:
the red cell membrane monoclonal antibody subcloning auxiliary structure comprises a base and a 96-hole cell culture plate, wherein the upper end surface of the base is provided with a rectangular groove, the 96-hole cell culture plate is arranged in the rectangular groove, and the rectangular groove divides the upper end surface of the base into two parts and forms two identification surfaces; the wells on the 96-well cell culture plate are divided into 12 columns, and the labels for displaying the cell numbers and the cell liquid sucking amounts in the third to seventh rows of the wells in the 12 columns are sequentially arranged on the two identification surfaces.
Further, after the 96-well cell culture plate is placed in the rectangular groove, the upper end face of the 96-well cell culture plate is flush with the upper end face of the base.
Further, the bottom surface of the rectangular groove is a frosted surface.
Further, the bottom surface of the base is a frosted surface, or anti-skid supporting feet are arranged on the bottom surface of the base.
Further, the base is made of acrylic material.
The utility model has the following beneficial effects:
the utility model is convenient to process and use. The 96-hole cell culture plate is placed in the rectangular groove, limiting dilution is carried out by comparing the parameter marks on the left side and the right side of the device through the cell count number, the limiting dilution is more convenient, the workload is lightened, the error caused by limiting dilution is prevented, the number of single groups is reduced, and the subcloning times are increased.
Drawings
Fig. 1 is a front view of the present utility model.
Fig. 2 is a top view of the base.
Description of the embodiments
The utility model is further described below with reference to the accompanying drawings.
Referring to fig. 1 and 2, the utility model relates to an auxiliary structure for monoclonal antibodies of erythrocyte membranes, which comprises a base 1 and a 96-hole cell culture plate 2, wherein the upper end surface of the base 1 is provided with a rectangular groove 11, the 96-hole cell culture plate 2 is arranged in the rectangular groove 11, and the rectangular groove 11 divides the upper end surface of the base 1 into two parts and forms two identification surfaces; the holes on the 96-hole cell culture plate 2 are divided into 12 columns, and the labels for displaying the cell numbers and the cell liquid sucking amount in the holes from the third row to the seventh row in the 12 columns are sequentially arranged on the two identification surfaces.
The label in the utility model is formed by directly inscribing the mark on the base 1 or directly fixing a nameplate with the mark on the base 1.
The rectangular groove 11 is used for placing a 96-hole cell culture plate, so that the 96-hole cell culture plate can be conveniently placed in a fitting manner; the 96-hole cell culture plate placed on the rectangular groove 11 is flush with the identification surface, the identification surface is provided with a parameter identification, and when counting is completed, the corresponding cell liquid amount which is ended and needs to be sucked by the double dilution hole can be intuitively obtained. And interval scale marks are arranged among the parameter marks corresponding to different holes and are used for distinguishing and preventing corresponding dislocation.
In addition, in order to prevent the 96-well cell culture plate from sliding in the rectangular groove 11 and the base 1 from sliding when placed on an ultra-clean bench, the bottom surface of the groove of the rectangular groove 11 is provided with a frosted surface. And setting the bottom surface of the base 1 as a frosted surface, or arranging anti-slip supporting feet on the bottom surface of the base 1.
When in use, firstly, the ultra-clean workbench is started and then is subjected to ultraviolet irradiation sterilization, and ventilation is not less than 15min after sterilization is completed; the auxiliary structure was then wiped with 75% alcohol, placed smoothly on top of an ultra clean bench, and 96 well cell culture plates to be diluted in a limited manner were placed in rectangular grooves 11.
The cell mass obtained by fusion or subcloning is blown uniformly, placed into a 1.5ml centrifuge tube added with culture medium in advance, and 200 μl of the first well of a 96-well cell culture plate is sucked after being blown uniformly again, and the remaining cell fluid is used for counting. For limiting dilution to be more convenient, the workload is reduced, the culture medium added into the centrifuge tube with 1.5ml can be added according to the size of the cell mass, and the counting result is controlled within 10. Limiting dilution is performed according to the counting result by controlling the parameter identification on the label, when the limiting dilution is performed to the required cell quantity, the corresponding liquid volume is sucked by a liquid-transferring gun, and the liquid is added into a culture medium containing feeder cells for plating.
The foregoing is merely a preferred embodiment of the utility model, and it should be noted that modifications could be made by those skilled in the art without departing from the principles of the utility model, which modifications would also be considered to be within the scope of the utility model.
Claims (5)
1. An auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane, which is characterized in that: the cell culture device comprises a base (1) and a 96-hole cell culture plate (2), wherein a rectangular groove (11) is formed in the upper end face of the base (1), the 96-hole cell culture plate (2) is arranged in the rectangular groove (11), and the rectangular groove (11) divides the upper end face of the base (1) into two parts and forms two identification surfaces; the holes on the 96-hole cell culture plate (2) are divided into 12 rows, and the labels for displaying the cell numbers and the cell liquid sucking amount in the holes from the third row to the seventh row in the 12 rows are sequentially arranged on the two identification surfaces.
2. The red blood cell membrane monoclonal antibody subcloning auxiliary structure of claim 1, wherein: after the 96-hole cell culture plate (2) is arranged in the rectangular groove (11), the upper end face of the 96-hole cell culture plate (2) is flush with the upper end face of the base (1).
3. The red blood cell membrane monoclonal antibody subcloning auxiliary structure of claim 1, wherein: the bottom surface of the rectangular groove (11) is a frosted surface.
4. The red blood cell membrane monoclonal antibody subcloning auxiliary structure of claim 1, wherein: the bottom surface of the base (1) is a frosted surface, or an anti-slip supporting leg is arranged on the bottom surface of the base (1).
5. The red blood cell membrane monoclonal antibody subcloning auxiliary structure of claim 1, wherein: and the base (1) is made of acrylic material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321102283.1U CN219807967U (en) | 2023-05-10 | 2023-05-10 | Auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321102283.1U CN219807967U (en) | 2023-05-10 | 2023-05-10 | Auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219807967U true CN219807967U (en) | 2023-10-10 |
Family
ID=88208593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321102283.1U Active CN219807967U (en) | 2023-05-10 | 2023-05-10 | Auxiliary structure for monoclonal antibody subcloning of erythrocyte membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219807967U (en) |
-
2023
- 2023-05-10 CN CN202321102283.1U patent/CN219807967U/en active Active
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