CN205556702U - Cell culture ware that immunofluorescence used - Google Patents
Cell culture ware that immunofluorescence used Download PDFInfo
- Publication number
- CN205556702U CN205556702U CN201620301179.9U CN201620301179U CN205556702U CN 205556702 U CN205556702 U CN 205556702U CN 201620301179 U CN201620301179 U CN 201620301179U CN 205556702 U CN205556702 U CN 205556702U
- Authority
- CN
- China
- Prior art keywords
- immunofluorescence
- cell culture
- diameter
- culture apparatus
- truncated cone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture ware that immunofluorescence used, it includes round platform shape cavity, two bottom surfaces of round platform shape cavity are not at the bottom of the confined opening, the less lower bottom surface and slide glass adhesion of round platform shape cavity area, the great last bottom surface edge of round platform shape cavity area is equipped with collar flange, the collar flange internal diameter is the same with the diameter of last bottom surface, the diameter of going up the bottom surface is the same with the aperture of training the diaphragm orifice, the utility model discloses the operator is difficult to follow the technical problem who cultivates the board bottom and take out with the slide glass when effectively, cheaply solving the immunofluorescence experiment, tests not restricted for use number moreover at every turn, can not cause the waste, has practiced thrift the experiment cost, has improved the efficiency of immunofluorescence experiment.
Description
Technical field
This utility model relates to technical field of medical instruments, and the cell in particular to a kind of immunofluorescence is trained
Support device.
Background technology
In Medical Molecular Biology is tested, immunofluorescence is a conventional and important experimental technique.This
The common practice of experiment is to be placed on by microscope slide bottom 6 well culture plates or 24 well culture plates, is then carrying glass
Inoculating cell on sheet.When after the complete antibody of cell incubation, microscope slide be taken out from culture plate, tabletting,
Finally take pictures under fluorescence microscope.But this experiment has the problem of a many experimenter of puzzlement,
It is exactly that microscope slide can be because negative pressure be sticked together bottom culture plate securely, it is difficult to be drawn off, and
And because culture hole is smaller, a lot of clamping tools are all difficult to operate.Experimenter be typically with tweezers or
Microscope slide is provoked by syringe needle, and this way is cumbersome and time consuming, and success rate is the highest.Test consumption at present
The representative of the solution problem above that material manufacturer provides isChamber slides system, but this product exists
Following deficiency: the most each incubator only has the culturing room of fixed number, if needing the training used in Shi Yan
Support room culturing room's number less than this consumptive material, then will necessarily cause waste；2. this consumptive material is imported product,
Expensive, and be only capable of using once, a lot of experimenters are difficult to bear.In sum, experimenter needs
One can solve the scheme that microscope slide takes out bottom culture plate simply, at a low price.
Summary of the invention
The purpose of this utility model is to provide the cell culture apparatus of a kind of immunofluorescence, solves immunity glimmering
In light experiment, microscope slide is difficult to the technical problem taken out bottom culture plate.
For achieving the above object, the cell culture apparatus of the immunofluorescence designed by this utility model, it is special
Different part is: it includes truncated cone-shaped chamber, and two bottom surfaces of described truncated cone-shaped chamber are not to be closed
Open bottom；
The less bottom surface of described truncated cone-shaped chamber area and microscope slide adhesion, described truncated cone-shaped chamber area
Bigger upper bottom surface edge is provided with collar flange, and described collar flange internal diameter is identical with the diameter of upper bottom surface,
The diameter of described upper bottom surface is identical with the aperture cultivating plate hole.
Further, the diameter of described upper bottom surface and the aperture of 24 orifice plates or 6 orifice plates are identical.Immunofluorescence
Usually use 24 orifice plates or 6 orifice plates, so the diameter that can design upper bottom surface is same, the most supporting make
With.
Further, the external diameter of described collar flange 2.5mm wider than internal diameter.This width design is in view of training
Support the interval between each hole of plate, if collar flange is the widest, when cell culture apparatus is placed in hole, respectively
Individual collar flange easily touches together.
Further, the height of described truncated cone-shaped chamber is 15mm.This height design is in view of culture plate
The degree of depth in hole, if truncated cone-shaped chamber height is too big, then microscope slide readily accesses the bottom cultivating plate hole.
Preferably, described collar flange and upper bottom surface edge integrated molding.
Preferably, described truncated cone-shaped chamber is the chamber that plastic material is made.
Operation principle of the present utility model is as follows:
The cell culture apparatus border seal of immunofluorescence, bottom surface utilizes cell growth without the material of impact
Material and microscope slide adhesion；Whole cell culture apparatus is hung on the hole of culture plate by collar flange.Cell is trained
Supporting device and provide the carrier needed for a growth to the cell being planted in the inside, culture plate is to cell culture apparatus
Provide an aseptic environment.After having hatched fluorescent antibody on microscope slide, by cell culture apparatus from training
Take out in supporting plate.Microscope slide will not be too strong with the adhesion of the bottom surface of Tissue Culture Plate, can be more easily
Both are separated by ground, do not interfere with microscope slide and shoot for tabletting and fluorescence.
The beneficial effects of the utility model are as follows:
When effectively, solving immunofluorescence experiment at a low price, operator is difficult to microscope slide bottom culture plate
The technical problem taken out, and test every time and be all not intended to use number, do not result in waste, save
Experimental cost, improves the efficiency of immunofluorescence experiment.
Accompanying drawing explanation
Fig. 1 is the perspective view of the cell culture apparatus of a kind of immunofluorescence；
Fig. 2 is that the cell culture apparatus of Fig. 1 is placed on the perspective view cultivated in plate hole；
Fig. 3 be Fig. 2 face structural representation；
Fig. 4 is the plan structure schematic diagram that the cell culture apparatus in Fig. 1 is placed on 6 well culture plates.
Detailed description of the invention
With specific embodiment, this utility model is described in further detail below in conjunction with the accompanying drawings:
The cell culture apparatus of immunofluorescence as shown in Figure 1 to Figure 3, it includes truncated cone-shaped chamber 1, institute
Two bottom surfaces stating truncated cone-shaped chamber 1 are the open bottom do not closed；
The less bottom surface 1a of described truncated cone-shaped chamber 1 area and microscope slide 2 adhesion, described truncated cone-shaped chamber
The upper bottom surface 1b edge integrated molding that room 1 area is bigger has collar flange 3, in described collar flange 3
Footpath is identical with the diameter of upper bottom surface 1b；
As shown in Figure 4, the diameter of described upper bottom surface 1b and the aperture of 6 orifice plates are identical；If used in 24
On orifice plate, the diameter of upper bottom surface 1b then designs according to the aperture of 24 orifice plates.
The external diameter 2.5mm wider than internal diameter of described collar flange 3.
The chamber that described truncated cone-shaped chamber 1 is made for plastic material, height is 15mm；
The cell culture apparatus border seal of the immunofluorescence of the present embodiment, bottom surface 1a utilizes cell raw
The long material without impact and microscope slide 2 adhesion；Whole cell culture apparatus hangs over 6 holes by collar flange 3
On the hole of plate.Cell culture apparatus give be planted in the inside cell provide one growth needed for carrier, 6
Orifice plate provides an aseptic environment to cell culture apparatus.After having hatched fluorescent antibody on microscope slide 2,
Cell culture apparatus is taken out in the hole of 6 orifice plates.Microscope slide 2 is viscous with the bottom surface 1a's of Tissue Culture Plate
Even will not be too strong, more easily both can be separated, not interfere with microscope slide 2 for tabletting and glimmering
Light shoots.
Claims (6)
1. the cell culture apparatus of an immunofluorescence, it is characterised in that: it includes truncated cone-shaped chamber (1),
Two bottom surfaces of described truncated cone-shaped chamber (1) are the open bottom do not closed；
The less bottom surface (1a) of described truncated cone-shaped chamber (1) area and microscope slide (2) adhesion, institute
State bigger upper bottom surface (1b) edge of truncated cone-shaped chamber (1) area and be provided with collar flange (3), described
Collar flange (3) internal diameter is identical with the diameter of upper bottom surface (1b), the diameter of described upper bottom surface (1b) with
The aperture cultivating plate hole is identical.
The cell culture apparatus of immunofluorescence the most according to claim 1, it is characterised in that: described
The diameter of upper bottom surface (1b) and the aperture of 24 orifice plates or 6 orifice plates are identical.
The cell culture apparatus of immunofluorescence the most according to claim 1 and 2, it is characterised in that:
The external diameter 2.5mm wider than internal diameter of described collar flange (3).
The cell culture apparatus of immunofluorescence the most according to claim 1 and 2, it is characterised in that:
The height of described truncated cone-shaped chamber (1) is 15mm.
The cell culture apparatus of immunofluorescence the most according to claim 1 and 2, it is characterised in that:
Described collar flange (3) and upper bottom surface (1b) edge integrated molding.
The cell culture apparatus of immunofluorescence the most according to claim 1 and 2, it is characterised in that:
Described truncated cone-shaped chamber (1) is the chamber that plastic material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620301179.9U CN205556702U (en) | 2016-04-12 | 2016-04-12 | Cell culture ware that immunofluorescence used |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620301179.9U CN205556702U (en) | 2016-04-12 | 2016-04-12 | Cell culture ware that immunofluorescence used |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205556702U true CN205556702U (en) | 2016-09-07 |
Family
ID=56810726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620301179.9U Expired - Fee Related CN205556702U (en) | 2016-04-12 | 2016-04-12 | Cell culture ware that immunofluorescence used |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205556702U (en) |
-
2016
- 2016-04-12 CN CN201620301179.9U patent/CN205556702U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100836827B1 (en) | Cell culture dish for the embryoid body formation from embryonic stem cells | |
| CN204474693U (en) | A kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish | |
| CN105378051B (en) | Structure for culturing cells | |
| CN204550529U (en) | A kind of microbial cultivation device | |
| CN205088253U (en) | Culture dish for it is biological experiments | |
| CN205556702U (en) | Cell culture ware that immunofluorescence used | |
| CN102876562A (en) | Microfluidic chip system for culture and multiplication behavior research of marine microalgae | |
| CN208562405U (en) | A kind of novel tissue culture plate | |
| CN217025936U (en) | Cell culture bottle | |
| CN203923211U (en) | A kind of co-culture of cells orifice plate | |
| CN204417516U (en) | The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope | |
| CN108504574A (en) | Culture bottle | |
| CN203429183U (en) | Three-dimensional cell culture plate | |
| CN208869583U (en) | A kind of fixation device of culture dish | |
| CN209039505U (en) | A kind of circular rotating Cell culture plate structure | |
| CN201241126Y (en) | Disposable microorganism test board | |
| CN203429184U (en) | Multi-adherent-cell coculture device | |
| CN205667149U (en) | A kind of culture system produced for Cordyceps | |
| CN202022941U (en) | Culture dish for maturing ovocyte of animal | |
| CN201400687Y (en) | Virus culture bottle | |
| CN205275566U (en) | Fixing device | |
| CN205803500U (en) | Tissue Culture Plate | |
| CN206902162U (en) | A kind of cell culture cell for epidermis model 3D cultures | |
| CN201525849U (en) | Amnion carrying lantern ring | |
| CN205681918U (en) | A kind of vaccination mechanism for cultivating edible fungi and strain container |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160907 Termination date: 20180412 |