CN108865798A - One kind splitting formula quantitative anti-skid visible surface sampling culture dish out - Google Patents
One kind splitting formula quantitative anti-skid visible surface sampling culture dish out Download PDFInfo
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- CN108865798A CN108865798A CN201810906641.1A CN201810906641A CN108865798A CN 108865798 A CN108865798 A CN 108865798A CN 201810906641 A CN201810906641 A CN 201810906641A CN 108865798 A CN108865798 A CN 108865798A
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- wall
- ware
- ware downside
- downside wall
- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses one kind to split formula quantitative anti-skid visible surface sampling culture dish out, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier, when multiple culture dishes are stacked placement, first protrusion and the second protrusion can limit the relative movement of two adjacent culture dish, observation window can make the observation of minute colony more convenient, what graduation mark can indicate agar medium pours into volume.
Description
Technical field
The present invention relates to a kind of experiment equipment, especially one kind to split formula quantitative anti-skid visible surface sampling culture dish out.
Background technique
Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware
The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab
Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face
To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity
Incomplete culture;In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism
Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working;These disadvantages
It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold
Easily cause the waste of human resources.When the culture dish pours into agar medium, the volume of pouring into of culture medium is usually operator
Depending on empirically, therefore the agar medium of different personnel pours into volume and may have larger difference, to influence relevant experiment
As a result.When the bacterium colony for the target culture that the culture medium in culture dish is grown is smaller, need to be amplified bacterium colony with microscope
By observe bacterium colony it is accurate in the form of, need to open in culture dish during magnifying bacterium colony, this may bring pollution
Or the leakage of harmful culture.
Summary of the invention
For disadvantages mentioned above, the purpose of the present invention is to provide the formulas out of splitting that a kind of structure is simple and convenient to operate quantitatively to prevent
Sliding visible surface sampling culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism, improve test
As a result accuracy improves working efficiency.
For splitting formula quantitative anti-skid visible surface sampling culture dish out, to reach the purpose, the technical side that the present invention uses
Case is:One kind splitting formula quantitative anti-skid visible surface sampling culture dish out, including ware bottom and the ware lid that is covered on ware bottom of relaxation,
It is characterized in that:The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, and the ware bottom includes
Bottom wall is at least one from bottom wall around the first ware downside wall holded up;The first ware downside wall is equipped at least one circle laterally
Crackle mouth, the transversal crack choma was around described first ware downside wall one week, the circle plane of the transversal crack mouth and institute
The plane for stating bottom wall is parallel;The first ware downside wall is divided into movable ware downside wall and fixation by the transversal crack mouth
Ware downside wall;The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, the cross
To crackle wall thickness less than the first ware downside wall;The movable ware downside wall is equipped at least one and splits perpendicular to the transverse direction
The movable ware downside wall of the longitudinal crack mouth of line mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall, the longitudinal direction
The thickness of crackle wall is less than the first ware downside wall;The diameter of the ware lid side wall is greater than the first ware downside wall;The bottom wall
Diameter be greater than the tegmental wall;The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, are less than institute
State the height of ware lid side wall;The bottom wall is additionally provided at least one first protrusion, and it is second convex that the tegmental wall is additionally provided at least one
It rises;It is additionally provided at least one transparent observation window in the tegmental wall, the observation window is in convex lens shape, and by the tegmental wall
Center thickeies and is formed;The first ware downside wall is additionally provided at least one graduation mark, and the graduation mark is around the
One ware downside wall one week, the circular flat of the graduation mark is parallel with the plane of the bottom wall.
Compared with the prior art, the beneficial effects of the invention are as follows:The longitudinal crack wall and transversal crack wall of the culture dish
Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made
Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall
Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use
Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian
The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously
Test result accuracy and working efficiency, when multiple culture dishes are stacked placement, the first protrusion and the second protrusion can
To limit the relative movement of two adjacent culture dish, observation window can make the observation of minute colony more convenient, and graduation mark can indicate
Agar medium pours into volume.
Detailed description of the invention
Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description
Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field
For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram for splitting formula quantitative anti-skid visible surface sampling culture dish out;Fig. 2 is the movable ware bottom side
The cross-sectional view of wall;Fig. 3 is the partial enlargement diagram of the first ware downside wall;Respectively number is respectively in figure:
1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 7 be the first ware downside wall, and 71 be movable ware bottom
Side wall, 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack wall, and 76 be fixed ware bottom
Side wall, 10 be the second protrusion, and 11 be the first protrusion, and 21 be observation window, and 22 be graduation mark.
Specific embodiment
It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention
The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on
Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.
In the discussion of specific embodiment, the shape for splitting formula quantitative anti-skid visible surface sampling culture dish out is preferable
Select it is round, still, those skilled in the art should be understood that it is suitable for such as ellipse, rectangle or its
It is suitable for the shape of culture dish.
The invention discloses one kind to split formula quantitative anti-skid visible surface sampling culture dish out, including ware bottom 2 and relaxation covering
In the ware lid 1 on ware bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid side wall 5 holded up, institute from tegmental wall 3
The ware bottom 2 stated includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4;The first ware downside wall
7 are equipped at least one circle transversal crack mouth 72, and the transversal crack mouth 72 is surround described first ware downside wall 7 one weeks, the cross
Circle plane to crackle mouth 72 is parallel with the plane of the bottom wall 4;The first ware downside wall 7 is by the transversal crack
Mouth 72 is divided into movable ware downside wall 71 and fixed ware downside wall 76;The movable ware downside wall 71 and the fixation ware bottom
The coupling part of side wall 76 is transversal crack wall 73, and the thickness of the transversal crack wall 73 is less than the first ware downside wall 7;Described
Movable ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74, the longitudinal crack mouth
The movable ware downside wall 71 of 74 two sides is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is less than the first ware bottom
Side wall 7.Features described above has determined the basic structure for splitting formula quantitative anti-skid visible surface sampling culture dish out, wherein longitudinal split
The thickness very little of line wall 75 and transversal crack wall 73 is easy to rupture under external force, makes the first ware downside wall 7 in longitudinal crack
It splits at mouth 74 and transversal crack mouth 72, movable ware downside wall 71 can be detached from from fixed ware downside wall 76 at this time.And first
The space that ware downside wall 7 and bottom wall 4 are formed is as culture inner cavity, when cultivating inner cavity by the enough agar mediums of injection(Institute
The liquid level for stating culture medium is higher than the transversal crack mouth 72), after the culture medium solidification, apply force to the activity
Ware downside wall 71 makes the first ware downside wall 7 split at longitudinal crack mouth 74 and transversal crack mouth 72, with removal activity ware bottom side
Wall 71, and then the height of fixed ware downside wall 76 is made to decrease below the plane of the agar medium, when use, will be described
Agar medium plane is directly covered in object under test surface, that is, can reach the purpose of sampling microorganisms from surface of object, avoid
The tedious steps eluted again after microorganism acquisition are carried out using cotton swab or filter paper, greatly simplify body surface microorganism
The process of sampling, and overcome and be easy the problem of being polluted by environmental microorganism in sampling process, while improving test result
Accuracy and working efficiency.
It is split in the embodiment of formula quantitative anti-skid visible surface sampling culture dish out described, the diameter of the bottom wall 4 is greater than
The diameter of the tegmental wall 3, the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, and ware lid side wall 5 can only be supported in
Bottom wall 4.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, are less than the ware
The height of lid side wall 5, this feature can be limited to be existed between activity ware downside wall 71 and tegmental wall 3 when ware lid 1 and the fastening of ware bottom 2
Certain gap prevents the agar medium from contacting with tegmental wall 3.
The culture dish further include at least one be located at the bottom wall 4 the first protrusion 11 and at least one be located at
Second protrusion 10 of the tegmental wall 3.First protrusion 11 is identical as the shape of the second protrusion 10.First protrusion 11 and the
The effect of two protrusions 10 is embodied in, and when multiple culture dishes are arranged to stacked structure, the first of a culture dish is convex
It plays 11 to cooperate with the second protrusion 10 of adjacent culture dish, one culture dish can be limited relative to the adjacent culture dish
It is mobile.
The tegmental wall 3 is additionally provided at least one transparent observation window 21, and the observation window 21 is in convex lens shape, and by
The center of the tegmental wall 3 thickeies and is formed, therefore the observation window 21 can be made micro- with the bacterium colony of amplification target culture
The observation of petite is more convenient, and can prevent the possible dirt because opening ware lid 1 and ware bottom 2 during observation bacterium colony
The leakage of dye or harmful culture.
The first ware downside wall 7 is additionally provided at least one graduation mark 22, and the graduation mark 22 is around the first ware bottom
Side wall 7 one weeks, the circular flat of the graduation mark 22 was parallel with the plane of the bottom wall 4;The graduation mark 22 can indicate
Agar medium pours into volume, to avoid pouring into the difference of volume because of personal experience's difference bring.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute
The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally
Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair
Bright protection scope.
Claims (1)
1. one kind splits formula quantitative anti-skid visible surface sampling culture dish out, including the ware lid that ware bottom and relaxation are covered on ware bottom,
It is characterized in that:The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up, the ware bottom packet from tegmental wall
It includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall;It is horizontal that the first ware downside wall is equipped at least one circle
To crackle mouth, the transversal crack choma around described first ware downside wall one week, the circle plane of the transversal crack mouth with
The plane of the bottom wall is parallel;The first ware downside wall is divided into movable ware downside wall and is consolidated by the transversal crack mouth
Determine ware downside wall;The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, described
The thickness of transversal crack wall is less than the first ware downside wall;The movable ware downside wall is equipped at least one perpendicular to the transverse direction
The movable ware downside wall of the longitudinal crack mouth of crackle mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall, described vertical
To crackle wall thickness less than the first ware downside wall;The diameter of the ware lid side wall is greater than the first ware downside wall;The bottom
The diameter of wall is greater than the tegmental wall;The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, are less than
The height of the ware lid side wall;The bottom wall be additionally provided at least one first protrusion, the tegmental wall be additionally provided at least one second
Protrusion;It is additionally provided at least one transparent observation window in the tegmental wall, the observation window is in convex lens shape, and by the lid
Wall center thickeies and is formed;The first ware downside wall is additionally provided at least one graduation mark, and the graduation mark is surround
First ware downside wall one week, the circular flat of the graduation mark is parallel with the plane of the bottom wall.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810906641.1A CN108865798A (en) | 2018-08-10 | 2018-08-10 | One kind splitting formula quantitative anti-skid visible surface sampling culture dish out |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810906641.1A CN108865798A (en) | 2018-08-10 | 2018-08-10 | One kind splitting formula quantitative anti-skid visible surface sampling culture dish out |
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Publication Number | Publication Date |
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CN108865798A true CN108865798A (en) | 2018-11-23 |
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CN201810906641.1A Withdrawn CN108865798A (en) | 2018-08-10 | 2018-08-10 | One kind splitting formula quantitative anti-skid visible surface sampling culture dish out |
Country Status (1)
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2018
- 2018-08-10 CN CN201810906641.1A patent/CN108865798A/en not_active Withdrawn
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Application publication date: 20181123 |