CN108893345A - One kind splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out - Google Patents

One kind splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out Download PDF

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Publication number
CN108893345A
CN108893345A CN201810981077.XA CN201810981077A CN108893345A CN 108893345 A CN108893345 A CN 108893345A CN 201810981077 A CN201810981077 A CN 201810981077A CN 108893345 A CN108893345 A CN 108893345A
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China
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wall
ware
ware downside
downside wall
culture dish
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CN201810981077.XA
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Chinese (zh)
Inventor
韩涛
韩德礼
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Individual
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Individual
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Priority to CN201810981077.XA priority Critical patent/CN108893345A/en
Publication of CN108893345A publication Critical patent/CN108893345A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses one kind to split the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier, the cooperation of first handgrip and the second handgrip can make ware bottom and ware lid more easily be turned on or fasten, observation window can make the observation of minute colony more convenient, what graduation mark can indicate agar medium pours into volume, radioactive ray can simplify the counting process of bacterium colony.

Description

One kind splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out
Technical field
The present invention relates to a kind of experiment equipment, especially one kind to split the hand-held fan-shaped count table surface sample training of formula quantitative visual out Support ware.
Background technique
Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity Incomplete culture；In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working；These disadvantages It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold Easily cause the waste of human resources.When the culture dish pours into agar medium, the volume of pouring into of culture medium is usually operator Depending on empirically, therefore the agar medium of different personnel pours into volume and may have larger difference, to influence relevant experiment As a result.When the bacterium colony for the target culture that the culture medium in culture dish is grown is smaller, need to be amplified bacterium colony with microscope By observe bacterium colony it is accurate in the form of, need to open in culture dish during magnifying bacterium colony, this may bring pollution Or the leakage of harmful culture.When the colony counts for the target culture that the culture medium in culture dish is grown are huge, bacterium colony Counting process it is then comparatively laborious.
Summary of the invention
For disadvantages mentioned above, the purpose of the present invention is to provide the formulas out of splitting that a kind of structure is simple and convenient to operate quantitatively may be used Depending on hand-held fan-shaped count table surface sample culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism, The accuracy of test result is improved, working efficiency is improved.
For splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out, to reach the purpose, the present invention is used Technical solution be：One kind splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out, including ware bottom and relaxation covering Ware lid on ware bottom, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side holded up from tegmental wall Wall, the ware bottom includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；The first ware bottom side Wall is equipped at least one circle transversal crack mouth, and the transversal crack choma around described first ware downside wall one week, split by the transverse direction The circle plane of line mouth is parallel with the plane of the bottom wall；The first ware downside wall is divided by the transversal crack mouth Movable ware downside wall and fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is Transversal crack wall, the thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall is equipped at least one Item is perpendicular to the longitudinal crack mouth of the transversal crack mouth, and the movable ware downside wall of longitudinal crack mouth two sides is by longitudinally splitting The connection of line wall, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than described first Ware downside wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the fixed ware downside wall The sum of height, less than the height of the ware lid side wall；The edge of the bottom wall is equipped at least one first handgrip, the tegmental wall Edge be equipped at least one second handgrip；At least one transparent observation window, the observation window are additionally provided in the tegmental wall In convex lens shape, and is thickeied by the tegmental wall center and formed；The first ware downside wall is additionally provided at least one Graduation mark, the graduation mark surround the first ware downside wall one week, the plane of the circular flat of the graduation mark and the bottom wall In parallel；In the bottom wall, the radioactive ray of bottom wall edge, the radioactive ray are extended to from bottom wall central point equipped at least two The bottom wall is divided into the fan-shaped region of at least two area equations.
Compared with the prior art, the beneficial effects of the invention are as follows：The longitudinal crack wall and transversal crack wall of the culture dish Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously The cooperation of test result accuracy and working efficiency, the first handgrip and the second handgrip can be such that ware bottom and ware lid is more easily turned on Or fasten, observation window can make the observation of minute colony more convenient, and graduation mark can indicate the volume of pouring into of agar medium, radiation Line can simplify the counting process of bacterium colony.
Detailed description of the invention
Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram for splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out；Fig. 2 is provided at institute State the schematic diagram of the radioactive ray of bottom wall；Fig. 3 is the cross-sectional view of the movable ware downside wall；Fig. 4 is first ware bottom The partial enlargement diagram of side wall；Respectively number is respectively in figure：
1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 7 be the first ware downside wall, and 71 be movable ware bottom Side wall, 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack wall, and 76 be fixed ware bottom Side wall, 8 be the second handgrip, and 9 be the first handgrip, and 21 be observation window, and 22 be graduation mark, and 23 be radioactive ray.
Specific embodiment
It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.
In the discussion of specific embodiment, the shape for splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out Shape preferably selects circle, still, those skilled in the art should be understood that it is suitable for such as ellipse, Rectangle or other shapes suitable for culture dish.
The invention discloses one kind to split the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out, including 2 He of ware bottom The ware lid 1 that relaxation is covered on ware bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid holded up from tegmental wall 3 Side wall 5, the ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4；Described first Ware downside wall 7 is equipped at least one circle transversal crack mouth 72, and the transversal crack mouth 72 is around the first ware downside wall 7 one Week, the circle plane of the transversal crack mouth 72 are parallel with the plane of the bottom wall 4；The first ware downside wall 7 is described Transversal crack mouth 72 be divided into movable ware downside wall 71 and fixed ware downside wall 76；The movable ware downside wall 71 with it is described Fixation ware downside wall 76 coupling part be transversal crack wall 73, the thickness of the transversal crack wall 73 is less than the first ware bottom side Wall 7；The movable ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74, it is described The movable ware downside wall 71 of 74 two sides of longitudinal crack mouth is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is small In the first ware downside wall 7.Features described above has determined that the formula quantitative visual out that splits holds fan-shaped count table surface sample culture dish Basic structure, wherein the thickness very little of longitudinal crack wall 75 and transversal crack wall 73, is easy to rupture under external force, makes first Ware downside wall 7 splits at longitudinal crack mouth 74 and transversal crack mouth 72, and movable ware downside wall 71 can be from fixed ware bottom at this time It is detached from side wall 76.And foot is injected when cultivating inner cavity as culture inner cavity in the space that the first ware downside wall 7 and bottom wall 4 are formed When the agar medium of amount（The liquid level of the culture medium is higher than the transversal crack mouth 72）, after the culture medium solidification, The movable ware downside wall 71 is applied force to, makes the first ware downside wall 7 at longitudinal crack mouth 74 and transversal crack mouth 72 It splits, with removal activity ware downside wall 71, and then the height of fixed ware downside wall 76 is made to decrease below the agar medium Plane, the agar medium plane is directly covered in object under test surface when use, that is, it is micro- to can reach body surface The purpose of biological sampling avoids and carries out the tedious steps eluted again after microorganism acquisition using cotton swab or filter paper, greatly Simplify the process of sampling microorganisms from surface of object, and overcome in sampling process be easy asked by what environmental microorganism polluted Topic, while improving test result accuracy and working efficiency.
In the embodiment for splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out, the bottom wall 4 Diameter is greater than the diameter of the tegmental wall 3, and the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, ware lid side wall 5 Bottom wall 4 can be supported in.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, small In the height of the ware lid side wall 5, this feature can limit activity ware downside wall 71 and tegmental wall 3 when ware lid 1 and the fastening of ware bottom 2 Between there are certain gaps, prevent the agar medium from contacting with tegmental wall 3.
The culture dish further includes the first handgrip 9 and at least one position that at least one is located at 4 edge of bottom wall The second handgrip 8 in 3 edge of tegmental wall.The presence of first handgrip 9 and the second handgrip 8 can make operator more easily use hand Ware lid 1 and ware bottom 2 are opened or fastened.
The tegmental wall 3 is additionally provided at least one transparent observation window 21, and the observation window 21 is in convex lens shape, and by The center of the tegmental wall 3 thickeies and is formed, therefore the observation window 21 can be made micro- with the bacterium colony of amplification target culture The observation of petite is more convenient, and can prevent the possible dirt because opening ware lid 1 and ware bottom 2 during observation bacterium colony The leakage of dye or harmful culture.
The first ware downside wall 7 is additionally provided at least one graduation mark 22, and the graduation mark 22 is around the first ware bottom Side wall 7 one weeks, the circular flat of the graduation mark 22 was parallel with the plane of the bottom wall 4；The graduation mark 22 can indicate Agar medium pours into volume, to avoid pouring into the difference of volume because of personal experience's difference bring.
In the bottom wall 4, the radioactive ray 23 at 4 edge of bottom wall are extended to from 4 central point of bottom wall equipped at least two, it is described Radioactive ray 23 bottom wall 4 is divided into the fan-shaped regions of at least two area equations；When the culture medium institute in culture dish When the colony counts of the target culture of growth are huge, the bacterium colony count results in a fan-shaped region can be used, multiplied by corresponding Multiple（In embodiment shown in Fig. 2, bottom wall 4 is equally divided into the fan-shaped region of 8 area equations, so multiple is 8）With It indicates all clump counts of culture dish, therefore simplifies the counting process of bacterium colony.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair Bright protection scope.

Claims (1)

1. one kind splits the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out, including ware bottom and relaxation are covered on ware bottom Ware lid, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, described Ware bottom includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；The first ware downside wall is equipped at least One circle transversal crack mouth, the transversal crack choma was around described first ware downside wall one week, the circle of the transversal crack mouth Plane is parallel with the plane of the bottom wall；The first ware downside wall is divided into movable ware bottom side by the transversal crack mouth Wall and fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack Wall, the thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall be equipped at least one perpendicular to The movable ware downside wall of the longitudinal crack mouth of the transversal crack mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall It connects, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than first ware bottom side Wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the height of the fixed ware downside wall The sum of, less than the height of the ware lid side wall；The edge of the bottom wall is equipped at least one first handgrip, the edge of the tegmental wall Equipped at least one the second handgrip；It is additionally provided at least one transparent observation window in the tegmental wall, the observation window is in convex lens Mirror shape, and thickeied by the tegmental wall center and formed；The first ware downside wall is additionally provided at least one graduation mark, For the graduation mark around the first ware downside wall one week, the circular flat of the graduation mark was parallel with the plane of the bottom wall；? The bottom wall, the radioactive ray of bottom wall edge are extended to equipped at least two from bottom wall central point, and the radioactive ray will be described Bottom wall be divided into the fan-shaped regions of at least two area equations.
CN201810981077.XA 2018-08-27 2018-08-27 One kind splitting the hand-held fan-shaped count table surface sample culture dish of formula quantitative visual out Withdrawn CN108893345A (en)

Priority Applications (1)

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Application publication date: 20181127

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