CN108841671A - One kind splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out - Google Patents
One kind splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out Download PDFInfo
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- CN108841671A CN108841671A CN201810981873.3A CN201810981873A CN108841671A CN 108841671 A CN108841671 A CN 108841671A CN 201810981873 A CN201810981873 A CN 201810981873A CN 108841671 A CN108841671 A CN 108841671A
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- wall
- ware
- ware downside
- downside wall
- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
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- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses one kind to split formula leakproof quantitative visual criss-cross method surface sampling culture dish out, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, observation window can make the observation of minute colony more convenient, and graduation mark can indicate the volume of pouring into of agar medium, and grid line can simplify the counting process of bacterium colony.
Description
Technical field
The present invention relates to a kind of experiment equipment, especially one kind to split formula leakproof quantitative visual criss-cross method surface sampling training out
Support ware.
Background technique
Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware
The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab
Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face
To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity
Incomplete culture；In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism
Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working；These disadvantages
It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold
Easily cause the waste of human resources.When the culture dish pours into agar medium, the volume of pouring into of culture medium is usually operator
Depending on empirically, therefore the agar medium of different personnel pours into volume and may have larger difference, to influence relevant experiment
As a result.When the bacterium colony for the target culture that the culture medium in culture dish is grown is smaller, need to be amplified bacterium colony with microscope
By observe bacterium colony it is accurate in the form of, need to open in culture dish during magnifying bacterium colony, this may bring pollution
Or the leakage of harmful culture.When the colony counts for the target culture that the culture medium in culture dish is grown are huge, bacterium colony
Counting process it is then comparatively laborious.
Summary of the invention
For disadvantages mentioned above, the purpose of the present invention is to provide the formula leakproofs out of splitting that a kind of structure is simple and convenient to operate to determine
Measure visual criss-cross method surface sampling culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism,
The accuracy of test result is improved, working efficiency is improved.
For splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out, to reach the purpose, the present invention is used
Technical solution be：One kind splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out, including ware bottom and relaxation covering
Ware lid on ware bottom, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side holded up from tegmental wall
Wall, the ware bottom includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；The first ware bottom side
Wall is equipped at least one circle transversal crack mouth, and the transversal crack choma around described first ware downside wall one week, split by the transverse direction
The circle plane of line mouth is parallel with the plane of the bottom wall；The first ware downside wall is divided by the transversal crack mouth
Movable ware downside wall and fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is
Transversal crack wall, the thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall is equipped at least one
Item is perpendicular to the longitudinal crack mouth of the transversal crack mouth, and the movable ware downside wall of longitudinal crack mouth two sides is by longitudinally splitting
The connection of line wall, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than described first
Ware downside wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the fixed ware downside wall
The sum of height, less than the height of the ware lid side wall；The culture dish further includes that at least one is holded up from bottom wall is circular
Second ware downside wall, the height of the second ware downside wall are less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid
Side wall, the first ware downside wall and the second ware downside wall are located at the same side of the bottom wall；At least one is additionally provided in the tegmental wall
Transparent observation window, the observation window are in convex lens shape, and are thickeied by the tegmental wall center and formed；Described
One ware downside wall is additionally provided at least one graduation mark, and the graduation mark is surround the first ware downside wall one week, the graduation mark
Circular flat is parallel with the plane of the bottom wall；In the bottom wall, the grid line equipped with vertical and horizontal orthogonal crossover, the grid
Bottom wall is divided into the region of several squares or approximating square, the area equation of the square area by line.
Compared with the prior art, the beneficial effects of the invention are as follows：The longitudinal crack wall and transversal crack wall of the culture dish
Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made
Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall
Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use
Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian
The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously
Test result accuracy and working efficiency.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, observation window can make micro-
The observation of petite is more convenient, and graduation mark can indicate the volume of pouring into of agar medium, and grid line can simplify the meter of bacterium colony
Number process.
Detailed description of the invention
Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description
Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field
For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram for splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out；Fig. 2 is provided at institute
State the schematic diagram of the grid line of bottom wall；Fig. 3 is the cross-sectional view of the movable ware downside wall；Fig. 4 is first ware bottom
The partial enlargement diagram of side wall；Respectively number is respectively in figure：
1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 6 be the second ware downside wall, and 7 be the first ware bottom side
Wall, 71 be movable ware downside wall, and 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack
Wall, 76 be fixed ware downside wall, and 13 be gap, and 21 be observation window, and 22 be graduation mark, and 23 be grid line.
Specific embodiment
It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention
The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on
Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.
In the discussion of specific embodiment, the shape for splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out
Shape preferably selects circle, still, those skilled in the art should be understood that it is suitable for such as ellipse,
Rectangle or other shapes suitable for culture dish.
The invention discloses one kind to split formula leakproof quantitative visual criss-cross method surface sampling culture dish out, including 2 He of ware bottom
The ware lid 1 that relaxation is covered on ware bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid holded up from tegmental wall 3
Side wall 5, the ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4；Described first
Ware downside wall 7 is equipped at least one circle transversal crack mouth 72, and the transversal crack mouth 72 is around the first ware downside wall 7 one
Week, the circle plane of the transversal crack mouth 72 are parallel with the plane of the bottom wall 4；The first ware downside wall 7 is described
Transversal crack mouth 72 be divided into movable ware downside wall 71 and fixed ware downside wall 76；The movable ware downside wall 71 with it is described
Fixation ware downside wall 76 coupling part be transversal crack wall 73, the thickness of the transversal crack wall 73 is less than the first ware bottom side
Wall 7；The movable ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74, it is described
The movable ware downside wall 71 of 74 two sides of longitudinal crack mouth is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is small
In the first ware downside wall 7.Features described above has determined the formula leakproof quantitative visual criss-cross method surface sampling culture dish out of splitting
Basic structure, wherein the thickness very little of longitudinal crack wall 75 and transversal crack wall 73, is easy to rupture under external force, makes first
Ware downside wall 7 splits at longitudinal crack mouth 74 and transversal crack mouth 72, and movable ware downside wall 71 can be from fixed ware bottom at this time
It is detached from side wall 76.And foot is injected when cultivating inner cavity as culture inner cavity in the space that the first ware downside wall 7 and bottom wall 4 are formed
When the agar medium of amount（The liquid level of the culture medium is higher than the transversal crack mouth 72）, after the culture medium solidification,
The movable ware downside wall 71 is applied force to, makes the first ware downside wall 7 at longitudinal crack mouth 74 and transversal crack mouth 72
It splits, with removal activity ware downside wall 71, and then the height of fixed ware downside wall 76 is made to decrease below the agar medium
Plane, the agar medium plane is directly covered in object under test surface when use, that is, it is micro- to can reach body surface
The purpose of biological sampling avoids and carries out the tedious steps eluted again after microorganism acquisition using cotton swab or filter paper, greatly
Simplify the process of sampling microorganisms from surface of object, and overcome in sampling process be easy asked by what environmental microorganism polluted
Topic, while improving test result accuracy and working efficiency.
It is split in the embodiment of formula leakproof quantitative visual criss-cross method surface sampling culture dish out described, the bottom wall 4
Diameter is greater than the diameter of the tegmental wall 3, and the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, ware lid side wall 5
Bottom wall 4 can be supported in.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, small
In the height of the ware lid side wall 5, this feature can limit activity ware downside wall 71 and tegmental wall 3 when ware lid 1 and the fastening of ware bottom 2
Between there are certain gaps, prevent the agar medium from contacting with tegmental wall 3.
The culture dish further includes that at least one surround the second ware downside wall 6 holded up, the first ware downside wall from bottom wall 4
7 and second ware downside wall 6 be located at the same side of the bottom wall 4.The diameter of the ware lid side wall 5 is greater than the first ware downside wall 7
Diameter, but less than the diameter of the second ware downside wall 6, this feature is limited when the ware lid 1 and the ware bottom 2 fastening, ware lid
Side wall 5 is fastened on the centre of the first ware downside wall 7 and the second ware downside wall 6 just.In addition, the height of the second ware downside wall 6 is small
In the ware lid side wall 5, this feature can prevent the second ware downside wall 6 from hindering the unlatching of the ware lid 1 with ware bottom 2
Or it fastens.First ware downside wall 7 and the second ware downside wall 6 and bottom wall 4 form gap 13, the gap 13 can collect to
The agar medium of spill and leakage, prevents the culture medium from overflowing from ware bottom 2 when injecting agar medium in culture dish.
The tegmental wall 3 is additionally provided at least one transparent observation window 21, and the observation window 21 is in convex lens shape, and by
The center of the tegmental wall 3 thickeies and is formed, therefore the observation window 21 can be made micro- with the bacterium colony of amplification target culture
The observation of petite is more convenient, and can prevent the possible dirt because opening ware lid 1 and ware bottom 2 during observation bacterium colony
The leakage of dye or harmful culture.
The first ware downside wall 7 is additionally provided at least one graduation mark 22, and the graduation mark 22 is around the first ware bottom
Side wall 7 one weeks, the circular flat of the graduation mark 22 was parallel with the plane of the bottom wall 4；The graduation mark 22 can indicate
Agar medium pours into volume, to avoid pouring into the difference of volume because of personal experience's difference bring.
In the bottom wall 4, the grid line 23 equipped with vertical and horizontal orthogonal crossover, bottom wall 4 is divided by the grid line 23
The region of several squares or approximating square, the area equation of the square area；When the culture medium institute in culture dish
When the colony counts of the target culture of growth are huge, the bacterium colony count results in a square area can be used, multiplied by phase
The multiple answered（In the embodiment shown in Figure 2, the side length of the square area is 1 centimetre, agar medium in ware bottom 2
Diameter is 10 centimetres, then its area is 78.5 square centimeters, so multiple is 78.5）To indicate all clump counts of culture dish,
Therefore the counting process of bacterium colony is simplified.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute
The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally
Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair
Bright protection scope.
Claims (1)
1. one kind splits formula leakproof quantitative visual criss-cross method surface sampling culture dish out, including ware bottom and relaxation are covered on ware bottom
Ware lid, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, described
Ware bottom includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；The first ware downside wall is equipped at least
One circle transversal crack mouth, the transversal crack choma was around described first ware downside wall one week, the circle of the transversal crack mouth
Plane is parallel with the plane of the bottom wall；The first ware downside wall is divided into movable ware bottom side by the transversal crack mouth
Wall and fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack
Wall, the thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall be equipped at least one perpendicular to
The movable ware downside wall of the longitudinal crack mouth of the transversal crack mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall
It connects, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than first ware bottom side
Wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the height of the fixed ware downside wall
The sum of, less than the height of the ware lid side wall；The culture dish further includes that at least one surround the second ware holded up from bottom wall
Downside wall, the height of the second ware downside wall are less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid side wall, the
One ware downside wall and the second ware downside wall are located at the same side of the bottom wall；At least one transparent sight is additionally provided in the tegmental wall
Window is examined, the observation window is in convex lens shape, and is thickeied by the tegmental wall center and formed；The first ware bottom side
Wall is additionally provided at least one graduation mark, and the graduation mark surround the first ware downside wall one week, the circular flat of the graduation mark
It is parallel with the plane of the bottom wall；In the bottom wall, the grid line equipped with vertical and horizontal orthogonal crossover, the grid line is by bottom wall
It is divided into the region of several squares or approximating square, the area equation of the square area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810981873.3A CN108841671A (en) | 2018-08-27 | 2018-08-27 | One kind splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810981873.3A CN108841671A (en) | 2018-08-27 | 2018-08-27 | One kind splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108841671A true CN108841671A (en) | 2018-11-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810981873.3A Withdrawn CN108841671A (en) | 2018-08-27 | 2018-08-27 | One kind splitting formula leakproof quantitative visual criss-cross method surface sampling culture dish out |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108841671A (en) |
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2018
- 2018-08-27 CN CN201810981873.3A patent/CN108841671A/en not_active Withdrawn
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Application publication date: 20181120 |