CN205974491U - Ration mar board - Google Patents
Ration mar board Download PDFInfo
- Publication number
- CN205974491U CN205974491U CN201620942553.3U CN201620942553U CN205974491U CN 205974491 U CN205974491 U CN 205974491U CN 201620942553 U CN201620942553 U CN 201620942553U CN 205974491 U CN205974491 U CN 205974491U
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- CN
- China
- Prior art keywords
- lid
- cut
- plate
- plate body
- mar
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a ration mar board, be in including mar needle, lid and setting the plate body of lid bottom, the inside surface of plate body is equipped with six culture hole, the upper surface of lid is equipped with six incisions, and two mar needle holder boards run through the incision with the plate body, the outer wall of lid is equipped with three infrared level instrument, laser emitter is installed on infrared level instrument's top, just infrared level instrument with laser emitter electric connection, this kind of ration mar board through setting up count square, infrared level instrument and mar scale mark, can make the mar position stable, and the width is stable, and the error of having avoided the human factor to arouse increases the stability of experiment, the selection of observing the place is controllable, and the subjective mistake of selecting is avoided as according to later can all observe same place at every turn to the scale, quantify more easily during at last according to area method statistical, subjective judgment when having avoided the analysis reduces the error, increases the credibility.
Description
Technical field
This utility model is related to life studies technical field, specially a kind of quantitative cut plate.
Background technology
Inoculate some cells in culture hole, treat that cell density reaches 80% about, drawn perpendicular to bottom surface with sharp thing
Trace, using normal saline flushing, removes floating cells, continues to cultivate the corresponding time, observes cut two edges distance, determine cell
Transfer ability.Existing scratch experiment technology is depended on experimenter and is being covered with using liquid-transfering gun pipette tips or other sharppointed article
Cut on the Tissue Culture Dish of cell or 6/12/24 orifice plate and realize.There is no certain objective criterion, thing in operation
The selection of product is more random.In last statistics, existing method mainly determines cell by observing cut two frontier distance
The speed of migration and ability.
Existing scratch experiment technology depends on the subjective random operation of experimenter, not only easily occurs in operating process
Error, and last result statistic processess rely primarily on observe cut two frontier distance and determine cell migration speed and
Ability, but cell is in transition process, is not neat advance, so being just easier appearance when delimiting migration border
Error, is also susceptible to the various deviations being caused by subjective and objective factor, leads to the stability of result and credibility to be beaten greatly
Discount, this is also the reason this experiment current is not gradually approved by the higher magazine of some influence abroad factors.This patent passes through
The specification of various equipment and using method and increased the quarter on culture dish and 6/12/24 orifice plate in specification scratch experiment
Degree, so that last statistical result does not depend solely on the distance on cut two border, can also rely on scored area area simultaneously
Decrement observe, there is certain objective criterion, not only reduce observe statistics difficulty, so that this result is had simultaneously
Can quantify, the characteristic such as objective and reproducible.
Utility model content
The purpose of this utility model is to provide a kind of quantitative cut plate, with solve in above-mentioned background technology propose ask
Topic.
For achieving the above object, this utility model provides following technical scheme：A kind of quantitative cut plate, including cut pin,
Plate lid and be arranged on the plate body of described Ban Gai bottom, the interior surface of described plate body is provided with six culture hole, described plate lid upper
Surface is provided with six otch, and two cut needle splints run through described otch and described plate body, and the outer wall of described plate lid is provided with three
Infrared ray gradienter, the top of described infrared ray gradienter is provided with laser beam emitting head, and described infrared ray gradienter and described
Laser beam emitting head is electrically connected with, and described infrared ray gradienter and described laser beam emitting head are all electrically connected with accumulator, described plate
The lower surface of body is provided with cut graduation mark and several count grid.
Further, described plate lid and described plate body match, and junction is provided with sealing ring.
Further, described culture hole is evenly distributed on the surface of described plate lid.
Further, it is fixedly connected with securing member by connecting rod between described infrared ray gradienter and described plate lid.
Further, the outer wall avris of described plate lid is provided with temporary repository, and the avris of described temporary repository is provided with
Hermatic door.Inside is provided with three dividing plates, and is connected through the hinge between described hermatic door and described temporary repository.
Compared with prior art, the beneficial effects of the utility model are：This kind of quantitative cut plate, by arranging counting side
Lattice, infrared ray gradienter and cut graduation mark, can make scoring position stable, width is stable, it is to avoid anthropic factor causes
Error, increases the stability of experiment；The selection observing place is controlled, and scale is as same with reference to can observe afterwards every time
Side, it is to avoid the subjective mistake selecting；Finally according to being easier during area-method statistical data to quantify, it is to avoid during analysis, subjectivity is sentenced
Disconnected, reduce error, increase credibility, and the setting of temporary repository, cell to be cultivated can be deposited the short time.
Brief description
Fig. 1 is overall structure diagram of the present utility model；
Fig. 2 is one of partial structural diagram of the present utility model；
Fig. 3 is the two of partial structural diagram of the present utility model；
In reference：1- hermatic door；2- temporary repository；3- dividing plate；4- otch；5- cut needle splint；6- plate lid；7-
Infrared ray gradienter；8- laser beam emitting head；9- securing member；10- connecting rod；11- plate body；12- counts grid；13- cut pin；14-
Culture hole.
Specific embodiment
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely description is it is clear that described embodiment is only a part of embodiment of this utility model rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of making creative work
The every other embodiment being obtained, broadly falls into the scope of this utility model protection.
Refer to Fig. 1-3, this utility model provides a kind of technical scheme：A kind of quantitative cut plate, including cut pin 13, plate
Lid 6 and the plate body 11 being arranged on described plate lid 6 bottom, the interior surface of described plate body 11 is provided with six culture hole 14, described plate
The upper surface of lid 6 is provided with six otch 4, and two cut needle splints 5 run through described otch 14 and described plate body 11, described plate lid 6
Outer wall be provided with three infrared ray gradienters 7, the top of described infrared ray gradienter 7 is provided with laser beam emitting head 8, and described red
Outside line level indicator 7 and described laser beam emitting head 8 are electrically connected with, described infrared ray gradienter 7 and described laser beam emitting head 8 all with store
Battery is electrically connected with, and the lower surface of described plate body 11 is provided with cut graduation mark and several count grid 12.
Further, described plate lid 6 and described plate body 11 match, and junction is provided with sealing ring.
Further, described culture hole 4 is evenly distributed on the surface of described plate lid 6.
Further, it is fixedly connected with securing member 9 by connecting rod 10 between described infrared ray gradienter 7 and described plate lid 6.
Further, the outer wall avris of described plate lid 6 is provided with temporary repository 2, and the avris of described temporary repository 2 is installed
There is hermatic door 1, inside is provided with three dividing plates 3, and is connected through the hinge between described hermatic door 1 and described temporary repository 2.
Operation principle：First, cell is placed in culture in culture hole 4, because the bottom of plate body 11 is provided with cut scale
Line, so, cut pin, in cut, can make scoring position stable, width is stable, it is to avoid the error that anthropic factor causes,
Increase the stability of experiment, and count the setting of grid 12, the selection in observation place can be made controlled, scale afterwards may be used as reference
To observe same place every time, it is to avoid the subjective mistake selecting.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with multiple changes in the case of without departing from principle of the present utility model and spirit, repair
Change, replace and modification, scope of the present utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. a kind of quantitative cut plate, including cut pin (13), plate lid (6) and the plate body (11) being arranged on described plate lid (6) bottom,
It is characterized in that：The interior surface of described plate body (11) is provided with six culture hole (14), and the upper surface of described plate lid (6) is provided with six
Individual otch (4), two cut needle splints (5) run through described otch (4) and described plate body (11), and the outer wall of described plate lid (6) sets
There are three infrared ray gradienters (7), the top of described infrared ray gradienter (7) is provided with laser beam emitting head (8), and described infrared
Line level indicator (7) and described laser beam emitting head (8) electric connection, described infrared ray gradienter (7) and described laser beam emitting head (8)
All it is electrically connected with accumulator, the lower surface of described plate body (11) is provided with cut graduation mark and several count grid (12).
2. a kind of quantitative cut plate according to claim 1 it is characterised in that：Described plate lid (6) and described plate body (11)
Match, and junction is provided with sealing ring.
3. a kind of quantitative cut plate according to claim 1 it is characterised in that：Described culture hole (14) is evenly distributed on institute
State the surface of plate lid (6).
4. a kind of quantitative cut plate according to claim 1 it is characterised in that：Described infrared ray gradienter (7) and described
It is fixedly connected with securing member (9) by connecting rod (10) between plate lid (6).
5. a kind of quantitative cut plate according to claim 1 it is characterised in that：The outer wall avris of described plate lid (6) is provided with
Temporary repository (2), the avris of described temporary repository (2) is provided with hermatic door (1), and inside is provided with three dividing plates (3), and institute
State and be connected through the hinge between hermatic door (1) and described temporary repository (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620942553.3U CN205974491U (en) | 2016-08-26 | 2016-08-26 | Ration mar board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620942553.3U CN205974491U (en) | 2016-08-26 | 2016-08-26 | Ration mar board |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205974491U true CN205974491U (en) | 2017-02-22 |
Family
ID=58035206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620942553.3U Expired - Fee Related CN205974491U (en) | 2016-08-26 | 2016-08-26 | Ration mar board |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205974491U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384715A (en) * | 2018-04-27 | 2018-08-10 | 上海长海医院 | A kind of cell scratch experiment culture dish and its application method |
| CN113984567A (en) * | 2021-10-21 | 2022-01-28 | 重庆长安汽车股份有限公司 | Method for testing scratch resistance of rubber material for vehicle |
-
2016
- 2016-08-26 CN CN201620942553.3U patent/CN205974491U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384715A (en) * | 2018-04-27 | 2018-08-10 | 上海长海医院 | A kind of cell scratch experiment culture dish and its application method |
| CN108384715B (en) * | 2018-04-27 | 2023-04-25 | 上海长海医院 | Culture dish for cell scratch experiment and use method thereof |
| CN113984567A (en) * | 2021-10-21 | 2022-01-28 | 重庆长安汽车股份有限公司 | Method for testing scratch resistance of rubber material for vehicle |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170222 Termination date: 20200826 |