CN108901842A - A method of induction Adiantum reniforme L var sinense Y. X. Lin sporinite - Google Patents
A method of induction Adiantum reniforme L var sinense Y. X. Lin sporinite Download PDFInfo
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- CN108901842A CN108901842A CN201810689882.5A CN201810689882A CN108901842A CN 108901842 A CN108901842 A CN 108901842A CN 201810689882 A CN201810689882 A CN 201810689882A CN 108901842 A CN108901842 A CN 108901842A
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- sporinite
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
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Abstract
The present invention provides a kind of method for inducing Adiantum reniforme L var sinense Y. X. Lin sporinite, including the induction of Adiantum reniforme L var sinense Y. X. Lin original foliage, and mature spore is grown the stripping and slicing after induced medium culture 58 days after original foliage；By the original foliage after stripping and slicing in carrying out Fiber differentiation on sporinite induction mixed-matrix, the induction step of Adiantum reniforme L var sinense Y. X. Lin sporinite can be completed.The induced medium is MS, and 1.0mg/L 6-BA, 0.1mg/L NAA, 3.0% sucrose of mass concentration, the mixture of 0.7% agar of mass concentration composition, the pH value of the mixture is 5.5~6.0.The sporinite induction mixed-matrix includes at least three kinds in river sand, peat, perlite and vermiculite.
Description
Technical field
The present invention provides a kind of method for inducing and cultivating of Adiantum reniforme L var sinense Y. X. Lin sporinite, specially Adiantum reniforme L var sinense Y. X. Lin sporinite
Matrix method for inducing and cultivating.
Background technique
Adiantum reniforme L var sinense Y. X. Lin also known as lotus leaf gold thread grass, belong to single leaf venushair fern system, pick up from Sichuan Province ten thousand by Xiong Wanzhong within 1975
County is named by Lin Youxing as novel species Adiantum reniforme L var sinense Y. X. Lin for 1980, is unique single blade profile plant.He is as Quaternary Period Relict Plant
The eyeball for having attracted each botanist at once is of great significance to the research of continental drift.In addition, Adiantum reniforme L var sinense Y. X. Lin is also
There is obvious pharmacological action to kidney liver and urinary system, being used as medicine in civil acquisition plant has quite long history.Currently, civil adopt
Collection and scientific research use even commercial operation that the plantation object plane is made to face bigger crisis.Therefore Adiantum reniforme L var sinense Y. X. Lin sporinite is efficiently induced,
It carries out species breeding and Protection significance is great.
It is red according to Institute of Botany, Chinese Academy of Sciences Guo on December 31 east in 1992；Stone mine；Chen Weilun is in periodical《Plant
Learn notification》" sterile culture of Adiantum reniforme L var sinense Y. X. Lin " text is pointed out only to can induce when lppmBA is added dropwise on mature original foliage and be embraced
Daughter is formed, required time about half a year or so.On July 1st, 2010 Institute Of Unity and Coherence In Writing Of Chongqing；Chongqing higher learning institutions' landscape flower engineering is ground
Center Zhang Zurong and Zhang Shaobin etc. are studied carefully in periodical《Agriculture of Anhui science》" the sporogenesis technology of spinulose tree fern and Adiantum reniforme L var sinense Y. X. Lin
The test result of research " shows that conversion ratio of 2 kinds of plants from gametophyte to sporinite is all very low, highest only 63.1%.2015
Year, 25 days 2 months Guangxi Zhuang Autonomous Region Yulin City Yuzhou District Yang Hui just applied《A kind of Adiantum reniforme L var sinense Y. X. Lin vitro Regeneration System》
Patent of invention show that GGB is in+0.43% agar of MS+0.8mg/L ZT+3.0% sucrose, differential medium that pH value is 5.8
Upper differentiation rate is 57.9%, not up to 60%, the space also further increased in induction differentiation.Moreover, GGB induction and
Sporinite sprouting carries out in pure inorganic chemical reagent culture medium, to test material in natural growth substrate condition
It explores limited.
Summary of the invention
The present invention overcomes deficiencies in the prior art, and especially sporinite induction time is too long or inductivity is not high
Disadvantage provides the method for efficiently induction Adiantum reniforme L var sinense Y. X. Lin sporinite, to repair the original ecological environment of Adiantum reniforme L var sinense Y. X. Lin, carries out
Environment big protection in the Yangtze river basin provides good basis.
For GGB is efficiently smoothly induced sporinite, first in MS+1.0mg/L 6-BA+0.1mg/L NAA+3.0% sugarcane
Sugared+0.7% agar, the culture medium that pH value is 5.5~6.0 are classified GGB, and quality and quantity reaches examination after GGB sprouting
Select bright-colored after testing requirement, the eugonic agglomerate for being cut into same size moves it in sporinite induced medium
Sporinite induction is carried out, specific technical solution is as follows：
A method of induction Adiantum reniforme L var sinense Y. X. Lin sporinite includes the following steps：
(1) Adiantum reniforme L var sinense Y. X. Lin original foliage induces, and mature spore is grown after original foliage through induced medium culture 50-70
Stripping and slicing after it；
It (2), can be complete by the original foliage after step (1) stripping and slicing in carrying out Fiber differentiation on sporinite induction mixed-matrix
At the induction step of Adiantum reniforme L var sinense Y. X. Lin sporinite.
Induced medium described in step (1) is MS, 1.0mg/L 6-BA, 0.1mg/L NAA, 3.0% sugarcane of mass concentration
Sugar, the mixture of 0.7% agar of mass concentration composition, the pH value of the mixture are 5.5~6.0.
The induction mixed-matrix of sporinite described in step (2) includes at least three in river sand, peat, perlite and vermiculite
Kind is with the mixture of any ratio.
It is river sand that sporinite, which induces the specific quality proportioning of each raw material of mixed-matrix,：Peat：Perlite=1：2：1；Or river
It is husky：Peat：Vermiculite=1：4：2；Or river sand：Peat：Perlite：Vermiculite=1：2：1/6：1/4；Or river sand：Perlite：Vermiculite
=1：5：3.
Induction Adiantum reniforme L var sinense Y. X. Lin sporinite practical technique advantage of the present invention：
Since nature culture and soilless culture substrate are more inorganization in terms of bulk density, air porosity and water holding
It learns environment to have a clear superiority, therefore nature culture and soilless culture substrate mixing are more advantageous to GGB and get rid of to pure inorganic chemistry ring
The dependence in border, reform of nature matrix environment ahead of time, more inorganic change can shorten sporinite sprout time 5-10 in mixed-matrix
It, improves germination rate 25-35%, and spore physical efficiency is grown up strong and sturdy rapidly, accelerates reproduction test process.
Any chemokine ingredient is free of in mixed-matrix, especially in organic matter species content, content of humic acid and ion
Exchange etc. is that inorganic environment is incomparable, so the merit of species can be maximally maintained, reduction was cultivated
Variation is generated in journey, and upper important in inhibiting is being mass produced.
After Adiantum reniforme L var sinense Y. X. Lin sporinite Germination And Seedling, field is primitively returned naturally, carries out restoration of the ecosystem construction and length
The big protection in river all has realistic meaning.
Detailed description of the invention
Fig. 1 is 1 Adiantum reniforme L var sinense Y. X. Lin GGB proliferative induction of embodiment.
Fig. 2 is the induction of 1 Adiantum reniforme L var sinense Y. X. Lin sporinite of embodiment.
Fig. 3 is 1 Adiantum reniforme L var sinense Y. X. Lin seedling replanting of embodiment growth figure, and a left side is the length of basin on Adiantum reniforme L var sinense Y. X. Lin seedling replanting
Gesture figure；The right side is the vigorous growing way figure of Adiantum reniforme L var sinense Y. X. Lin growth of seedling.
Specific embodiment
Embodiment 1
Explant processing, original foliage and GGB induction
Acquisition current year generate be seeded in after the disinfection of ripe spore MS+0.5mg/L 6-BA+0.1mg/L NAA+3.0% sucrose+
0.7% agar, pH value are to carry out GGB induction, induction training after growing within 30 days or so original foliage in 5.5~6.0 initial culture bases
Supporting base is+0.7% agar of MS+1.0mg/L 6-BA+0.1mg/L NAA+3.0% sucrose, and pH value is 5.5~6.0；
The type of matrix selects and proportion
The type and proportion of matrix are sporinite success induction key points.Sporinite induce mixed-matrix according to
After certain proportion prepares, be added pure water reconciled, amount of water with matrix hold it is naturally agglomerating in hand be advisable, matrix divide
Dress thickness is advisable with 1-2cm, and the work that reconciles sterilizes in high-pressure sterilizing pot after the completion, common pressure 0.15MPa, temperature 121
DEG C, 20~40min of sterilization time.
The mixed-matrix mainly has river sand, peat, perlite and four kinds of vermiculite, and specific proportion is 1. river sand：Mud
Charcoal：Perlite=1：2：1 2. river sand：Peat：Vermiculite=1：4：2 3. river sands：Peat：Perlite：Vermiculite=1：2：1/6：1/4
4. river sand：Perlite：Vermiculite=1：5：3.
Test result show after 50 days or so Fiber differentiations sporinite in 3. river sand：Peat：Perlite：Leech
Stone=1：2：1/6：Inductivity highest, reaches 81.7% in 1/4 mixed-matrix, in 4. river sand：Perlite：Vermiculite=1：5：3
Mixed-matrix in inductivity it is minimum, only 56.7%.Detailed Experimental result see the table below.
60d induces situation in different mixed-matrix miospore bodies after inoculation
Claims (4)
1. a kind of method for inducing Adiantum reniforme L var sinense Y. X. Lin sporinite, which is characterized in that include the following steps：
（1）Mature spore is grown the stripping and slicing after induced medium culture 58 days after original foliage by the induction of Adiantum reniforme L var sinense Y. X. Lin original foliage；
（2）By step（1）Lotus leaf can be completed in carrying out Fiber differentiation on sporinite induction mixed-matrix in original foliage after stripping and slicing
The induction step of venushair fern sporinite.
2. the method for induction Adiantum reniforme L var sinense Y. X. Lin sporinite described in claim 1, which is characterized in that step（1）The induction
Culture medium is MS, and 1.0mg/L 6-BA, 0.1mg/L NAA, 3.0% sucrose of mass concentration, what 0.7% agar of mass concentration formed mixes
Object is closed, the pH value of the mixture is 5.5~6.0.
3. the method for induction Adiantum reniforme L var sinense Y. X. Lin sporinite described in claim 1, which is characterized in that step（2）The spore
Body induction mixed-matrix includes at least three kinds mixtures with any ratio in river sand, peat, perlite and vermiculite.
4. the method for induction Adiantum reniforme L var sinense Y. X. Lin sporinite described in claim 1, which is characterized in that sporinite induces mixed-matrix
The specific quality proportioning of each raw material is river sand：Peat：Perlite=1：2：1；Or river sand：Peat：Vermiculite=1：4：2；Or river sand：Mud
Charcoal：Perlite：Vermiculite=1：2：1/6：1/4；Or river sand：Perlite：Vermiculite=1：5：3.
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| CN201810689882.5A CN108901842B (en) | 2018-06-28 | 2018-06-28 | Method for inducing sporophyte of Adiantum didatus |
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| CN201810689882.5A CN108901842B (en) | 2018-06-28 | 2018-06-28 | Method for inducing sporophyte of Adiantum didatus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226385A (en) * | 2019-06-11 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of type of seeding of pteridophyte |
| CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
| CN112931212A (en) * | 2021-03-29 | 2021-06-11 | 江苏蕨美植物科技有限公司 | Method for breeding adiantum ladder |
| CN113133385A (en) * | 2020-01-20 | 2021-07-20 | 上海辰山植物园 | Spore propagation method of rare or endangered lotus leaf Adiantum |
| CN115500260A (en) * | 2022-09-09 | 2022-12-23 | 中国长江三峡集团有限公司 | Method for efficiently breeding special rare plant nelumbo nucifera gaertn by simulating field environment |
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| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226385A (en) * | 2019-06-11 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of type of seeding of pteridophyte |
| CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
| CN113133385A (en) * | 2020-01-20 | 2021-07-20 | 上海辰山植物园 | Spore propagation method of rare or endangered lotus leaf Adiantum |
| CN112931212A (en) * | 2021-03-29 | 2021-06-11 | 江苏蕨美植物科技有限公司 | Method for breeding adiantum ladder |
| CN115500260A (en) * | 2022-09-09 | 2022-12-23 | 中国长江三峡集团有限公司 | Method for efficiently breeding special rare plant nelumbo nucifera gaertn by simulating field environment |
| GB2622316A (en) * | 2022-09-09 | 2024-03-13 | China Three Gorges Corp | Method for simulating high-efficiently breeding unique rare Adiantum nelumboides in wild environment |
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