CN103081745B - Pteridophyte seedling raising device - Google Patents
Pteridophyte seedling raising device Download PDFInfo
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- CN103081745B CN103081745B CN201310041580.4A CN201310041580A CN103081745B CN 103081745 B CN103081745 B CN 103081745B CN 201310041580 A CN201310041580 A CN 201310041580A CN 103081745 B CN103081745 B CN 103081745B
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- 238000009331 sowing Methods 0.000 claims abstract description 48
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Abstract
The invention discloses a pteridophyte seedling raising device. The pteridophyte seedling raising device comprises a seedling raising basin, a sowing clapboard, and a culture medium pushout constructional element. The seedling raising basin comprises a basin body (2) and a cover body (3) which is matched with the basin body. The basin body is composed of a bottom wall and side walls. Through-holes are formed in the bottom wall. The cover body is composed of a top wall and side walls. Convection holes are formed in the side wall of the cover body. The sowing clapboard (4) is arranged in the basin body and is composed of clapboard side walls and side walls of griddings arranged inside the clapboard side walls. The culture medium pushout constructional element (5) comprises a base seat and a convex circular cylinder which is arranged on the base seat and matched with the shape of gridding cavities. The pteridophyte seedling raising device can improve efficiency of seedling and transplanting, reduce cross infection probability between sporophytes, reduce damage of gametophytes and sporophytes in transplanting process, and has the advantages that cultivating room is made full use of, stability of development environment of spores and seeding transplanting process standardization are guaranteed, management cost is reduced and the pteridophyte seedling raising device is suitable for pteridophyte seedling standardization and large-scale production.
Description
Technical field
The present invention relates to a kind of pteridophyte seedling nursing device.
Background technology
China's pteridophyte resource is very abundant, has 2456 kinds, accounts for 1/5 of world's fern sum, and wherein 1218 kinds is endemic species.That a lot of pteridophytes have is medicinal, eat, view and admire and the ecological value.Because fern is a kind of more original vascular plant, gametophytic generation (sexual generation) is in weak tendency, grow and be fertilized and be higher to requirement for environmental conditions, so the cultivation of pteridophyte breeding is difficult compared with spermatophyte, this has also restricted the exploitation to pteridophyte greatly.Along with people are to progressively the understanding and increase day by day to the pteridophyte demand such as medicinal, edible of pteridophyte economic worth, caused the excessive predation to wild fern resource.Add that environment in recent years deterioration, vegetation deterioration, humidity decline, and have further aggravated the exhaustion of fern resource, and have made a variety of ferns endangered.Therefore, greatly develop fern cultivation propagation technique extremely urgent, be not only conducive to the sustainable use of fern resource, and significant to the protection of wild fern germplasm.
Except some cultivars, most ferns can produce the spore of enormous amount, are undertaken that fern breeding is convenient and swift and efficiency is high by sowing spore.But the spore of pteridophyte is little more a lot of than seed, if use common seedling nursing device in reproductive process, divide seedling process to be difficult to standardization, thereby the loss that easily varies with each individual and affect production efficiency and increase seedling while transplanting, and spore germination and grow very responsively to the variation of environmental condition, common seedling nursing device is difficult to meet the demands.
Summary of the invention
The object of this invention is to provide a kind of pteridophyte seedling nursing device.
Pteridophyte seedling nursing device provided by the invention, comprises seedling-growing container, sowing dividing plate and culture matrix release member;
The lid 3 that described seedling-growing container comprises basin body 2 and mates with it; Described basin body is the cavity being comprised of diapire and basin body sidewall, and diapire is provided with an above through hole; Described lid is the cavity being comprised of roof and covered body wall, and covered body wall is provided with the convection holes for Air Flow;
Described sowing dividing plate 4 is located in described basin body, for by dividing plate sidewall be located at the cavity body with some grids chamber that the grid sidewall in dividing plate sidewall forms;
Described culture matrix is released member 5 and is comprised base and be located on described base and the equal form fit in all grids described sowing dividing plate chamber and some convex columns one to one.
The diameter of described through hole can be 3-6mm, specifically can be 5mm.
The diameter of described convection holes can be 2-3mm, specifically can be 2mm.
Described basin body is specially rectangular-shaped, diapire and four basin body sidewalls, consists of.The length of the inner chamber of described basin body specifically can be 163mm, width and specifically can be 153mm, highly specifically can be 59mm.Described lid is specially rectangular-shaped, roof and four covered body walls, consists of.The length of the inner chamber of described lid specifically can be 163mm, width and specifically can be 153mm, highly specifically can be 29mm.On described diapire, can evenly be provided with 8 through holes.On described covered body wall, can be provided with two groups of convection holes, on each covered body wall one group, two groups of convection holes are symmetrical arranged, and every group of convection holes comprises 6 convection holes.Specifically, in every group of convection holes, in the middle of 1 convection holes is positioned at, it is around around other 5 convection holes.
Acting as of seedling-growing container basin body: sprouting and seedling space that the spore of pteridophyte is provided.
Acting as of seedling-growing container lid: the spore germination of pteridophyte needs moistening environment, but complete totally enclosed environment easily makes cultivation matrix temperature raise, and grows algae, fungi etc., and is unfavorable for keeping the fresh of air; On the one hand, the existence of seedling-growing container lid has guaranteed that seedling-growing container has sealing to a certain degree, has overcome the tradition vessel of growing seedlings and without sealing, has caused moisture rapid evaporation, the problem that spore cannot normally be sprouted; On the other hand, seedling-growing container lid is provided with two groups of corresponding convection holes, thereby seedling-growing container within forms cross-ventilation, making can be relatively constant in humidity to the extremely responsive pteridophyta spore of air humidity, in the fresh environment of air, sprout smoothly, can avoid using and have the tradition of the lid vessel of growing seedlings often to need uncap to ventilate to cause the problem that moisture fluctuation is large, avoided the uncontrollability of operation, greatly improve the efficiency of management, reduced management cost, also avoided traditional breeding method vessel to seal completely and caused spore and the perishable shortcoming of gametophyte.
Described sowing dividing plate can be rectangular-shaped.The outer length of described sowing dividing plate specifically can be 161mm, outer width specifically can be 151mm, and outer height specifically can be 15mm, has the grid chamber that 240 internal diameters are 9 * 9mm.
Sowing the acting as of dividing plate: the spore quantity that (1) is scattered in while making to sow in each lattice is roughly the same, easily causes the shortcoming of damage to gametophyte and sporophyte when intensive overlapping on affects growth and seedling and minute seedling together after having overcome traditional seeding method gametophyte and growing up; (2) when transplanting, the seedling in each lattice does as a whole transplanting, guarantees that the size and number of every group planting strain is substantially suitable, has guaranteed the standardization of the process of transplanting, and can not be subject to the impact of human factor.
The base that described culture matrix is released member can be rectangular-shaped.The outer length of described base specifically can be that 180mm, outer width specifically can be 170mm, outer height is specially 5mm, on base, distance edge 11mm is to be provided with 240 rectangular-shaped convex columns in interior scope, the external diameter of each convex column is 8 * 8mm, highly for 20mm, the vertical range of every two convex column outers is 2mm.
Culture matrix is released acting as of member: use culture matrix is released member and take out the matrix with seedling from sowing dividing plate, has not only overcome the shortcoming of easily losing its root while digging out seedling with transplanting instrument, and has greatly improved transplanting efficiency.
Described pteridophyte seedling nursing device also can comprise for placing the pallet 1 of described seedling-growing container.
Described pallet specifically can be rectangular-shaped, and outer length specifically can be 480mm, outer width specifically can be 360mm.Four sidewalls of described pallet are bottom-up can be outward-dipping.Each pallet can be placed 6 seedling-growing containers.The culturing rack of every layer of 500 * 1200mm can be placed 3 pallets, thereby makes full use of culturing rack space, and is convenient to moving fast of a plurality of seedling-growing containers.
Acting as of pallet: pack into after soil in (1) seedling-growing container, can add water in pallet, seedling-growing container is directly put into pallet and make soil water suction; (2) in growing process, need to water in seedling-growing container, pallet also can be avoided the mud pollution culturing rack oozing out from seedling-growing container bottom; (3) be convenient to once move fast a plurality of seedling-growing containers.
The size of pallet and seedling-growing container is reasonable in design, can make full use of the space of whole layer culturing rack.
The material of seedling-growing container specifically can be the transparent PP plastics into stable chemical nature.The material of sowing dividing plate and culture matrix release member specifically can be the PP plastics of stable chemical nature.The material of pallet specifically can be the PVC plastics of stable chemical nature.
Arbitrary described pteridophyte seedling nursing device can be used for pteridophyte and grows seedlings above.
Described pteridophyte can be wall fern order plant, specifically can Drynariaceae plant, more specifically can be Mongolian oak Cyclosorus plant, and more specifically can be Mongolian oak fern.Described pteridophyte can be wall fern order plant, specifically can Cyatheaceae plant, more specifically can be spinulose tree fern.Described pteridophyte can be Pteridaceae plant, specifically can be Pteris plant, more specifically can be white jade brake fern.Described pteridophyte can be wall fern order plant, specifically can be Dryopteridaceae plant, more specifically can be polystichun family plant, more specifically can be Cyrtomium plant, more specifically can be cyrtomium caryotideumPresl.
In sum; pteridophyte seedling nursing device provided by the invention; can improve seedling and transplanting efficiency; reduce cross-infection probability between sporophyte; reduce the damage that transplanting process gametophyte and sporophyte are subject to; have reasonable in design, make full use of culture space, guarantee the stability of spore development environment and the standardization of sowing transplanting process, reduce the advantages such as management cost, be particularly suitable for standardization and the large-scale production of pteridophyte seedling.
Accompanying drawing explanation
Fig. 1 is the front view of sowing basin body.
Fig. 2 is the front view of sowing basin lid.
Fig. 3 is the front view of sowing dividing plate.
Fig. 4 is the front view that culture matrix is released member
Fig. 5 is the front view of sowing dividing plate and culture matrix release Components Composition state.
Fig. 6 is the assembling mode schematic diagram of sowing basin lid, sowing dividing plate and sowing basin body.
Fig. 7 is the front view of pallet.
Fig. 8 is the front view of pallet and sowing basin assembled state.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.
Pteridophyte seedling nursing device provided by the invention, comprises seedling-growing container, sowing dividing plate and culture matrix release member; The lid 3 that described seedling-growing container comprises basin body 2 and mates with it; Described basin body is the cavity being comprised of diapire and basin body sidewall, and diapire is provided with an above through hole; Described lid is the cavity being comprised of roof and covered body wall, and covered body wall is provided with the convection holes for Air Flow; Described sowing dividing plate 4 is located in described basin body, for by dividing plate sidewall be located at the cavity body with some grids chamber that the grid sidewall in dividing plate sidewall forms; Described culture matrix is released member 5 and is comprised base and be located on described base and the equal form fit in all grids described sowing dividing plate chamber and some convex columns one to one.The diameter of described through hole can be 3-6mm, specifically can be 5mm.The diameter of described convection holes can be 2-3mm, specifically can be 2mm.Described basin body is specially rectangular-shaped, diapire and four basin body sidewalls, consists of.The length of the inner chamber of described basin body specifically can be 163mm, width and specifically can be 153mm, highly specifically can be 59mm.The thickness of basin body sidewall specifically can be 1mm.Described lid is specially rectangular-shaped, roof and four covered body walls, consists of.The length of the inner chamber of described lid specifically can be 163mm, width and specifically can be 153mm, highly specifically can be 29mm.The thickness of covered body wall specifically can be 1mm.Cap side-wall can outwards routed up the edge of 1mm apart from 2mm place, edge, and for interlocking with basin body, folding with convenience, can reach sealing effectiveness again.On described diapire, can evenly be provided with 8 through holes.On described covered body wall, can be provided with two groups of convection holes, on each covered body wall one group, two groups of convection holes are symmetrical arranged, and every group of convection holes comprises 6 convection holes.Specifically, in every group of convection holes, in the middle of 1 convection holes is positioned at, it is around around other 5 convection holes.Described sowing dividing plate can be rectangular-shaped.The outer length of described sowing dividing plate specifically can be 161mm, outer width specifically can be 151mm, and outer height specifically can be 15mm, has the grid chamber that 240 internal diameters are 9 * 9mm (16 * 15).The thickness of dividing plate sidewall and grid sidewall specifically can be 1mm.The base that described culture matrix is released member can be rectangular-shaped.The outer length of described base specifically can be that 180mm, outer width specifically can be 170mm, outer height is specially 5mm, on base, distance edge 11mm is to be provided with 240 rectangular-shaped convex columns (16 * 15) in interior scope, the external diameter of each convex column is 8 * 8mm, highly for 20mm, the vertical range of every two convex column outers is 2mm.Described device also can comprise for placing the pallet 1 of described seedling-growing container.Described pallet specifically can be rectangular-shaped, and outer length specifically can be 480mm, outer width specifically can be 360mm.Each pallet can be placed 6 seedling-growing containers.Fig. 1 is the front view of sowing basin body.Fig. 2 is the front view of sowing basin lid.Fig. 3 is the front view of sowing dividing plate.Fig. 4 is that culture matrix is released the front view that front view Fig. 5 of member is sowing dividing plate and culture matrix release Components Composition state.Fig. 6 is the assembling mode schematic diagram of sowing basin lid, sowing dividing plate and sowing basin body.Fig. 7 is the front view of pallet.Fig. 8 is the front view of pallet and sowing basin assembled state.The material of seedling-growing container specifically can be the transparent PP plastics into stable chemical nature.The material of sowing dividing plate and culture matrix release member specifically can be the PP plastics of stable chemical nature.The material of pallet specifically can be the PVC plastics of stable chemical nature.
The method that adopts pteridophyte seedling nursing device provided by the invention to carry out spore sowing and grow seedlings is exemplified below:
1, in seedling-growing container basin body, add the matrix of about 20mm thickness (2 parts by volume peat soils and 1 parts by volume element sand to be mixed, pack cloth bag at 121 ℃ of sterilizing 20min of high-pressure sterilizing pot), flatten, then in basin body, put into sowing dividing plate, and insert matrix until covered sowing dividing plate in sowing dividing plate, again matrix is flattened gently, scrape off the matrix that surpasses sowing dividing plate.
2, in pallet, add the dark water in 20mm left and right, then seedling-growing container basin body is put into pallet, matrix is fully absorbed water, then remove remaining water in pallet.
3,10mg Mongolian oak fern spore is added in 100ml water, obtain spore suspension.
4, spore suspension step 3 being obtained is evenly sprayed on the stromal surface (every 100ml spore suspension can spray 10 sowing basins) in the seedling-growing container basin body that step 3 obtains, and covers seedling-growing container lid, finishes writing label, 25 ± 2 ℃ of dark culturing 48 hours.
5, after completing steps 4, pallet is placed on culturing rack, (illumination every day 16 hours, intensity of illumination is 30-50 μ molm in 25 ± 2 ℃ of cultivations
-2s
-1).
Cultivate the spore that the green point occurring in the matrix of left and right for 15 days is sprouting.Cultivate after 50-60 days and form a large amount of heart-shaped prothallium, regularly uncap with watering can spray a small amount of water in gametophyte surface to promote fertilization process.Cultivate 90-100d left and right and start to form sporophyte, in each lattice of dividing plate, approximately can form 2-3 strain, each seedling-growing container can obtain about 500-700 strain sporophyte seedling, every layer of culturing rack can obtain about 9000-12000 strain seedling, can use some rare fertilizer this period, or spray once with the every 7-10d of 1/2MS nutrient solution, be beneficial to the growth of seedling.After sporophyte seedling grows 2 true leaves that about 20mm is high, dividing plate body with seedling and matrix is taken out, the unnecessary matrix in dividing plate body below is scraped off, with dividing plate top, buckle and from below, insert dividing plate body by matrix and seedling release, matrix in each lattice and seedling are planted in preprepared vinyl disc or foveola dish, water itself and matrix (3 parts of pure bog moss or bog mosses, 1 part of mixed preparing of perlite) are fully merged, seedling replanting survival rate is 90%.
Adopt pteridophyte seedling nursing device provided by the invention and said method multiple pteridophyte can be carried out to spore sowing and grows seedlings, the transplanting survival rate of spinulose tree fern reaches 89%, and the transplanting survival rate of white jade brake fern reaches 96%, and the transplanting survival rate of cyrtomium caryotideumPresl reaches 92%.
The method that adopts traditional fern seed seedling nursing device to carry out spore sowing and grow seedlings is exemplified below:
1, the preparation of vessel
Select suitable vessel (as plastics cutlery box, vinyl disc), a plurality of apertures are beaten in its bottom, be convenient to immersion and draining.
2, matrix (is mixed 2 parts by volume peat soils and 1 parts by volume element sand, pack cloth bag at 121 ℃ of sterilizing 20min of high-pressure sterilizing pot) pack in the vessel that step 1 obtains, thickness leveling the appropriate compacting of about 30mm, saturated with water, with watering can, stromal surface is sprayed to 1 time water again, be beneficial to and make soil crack up.
3,10mg Mongolian oak fern spore is added in 100ml water, obtain spore suspension.
4, spore suspension step 3 being obtained is evenly sprayed on the stromal surface of step 2.Cover sowing basin lid, finish writing label, 25 ± 2 ° of C dark culturing 48 hours, (illumination every day 16 hours, intensity of illumination is 30-50 μ molm then to transfer to 25 ± 2 ℃ of cultivations of culturing room
-2s
-1).
Cultivate the spore that the green point occurring in the matrix of left and right for 15 days is sprouting.Cultivate after 50-60 days and form a large amount of heart-shaped prothallium, regularly uncap with watering can spray a small amount of water in gametophyte surface to promote fertilization process.Cultivate 90-100d left and right and start to form sporophyte, can use some rare fertilizer this period, or spray once with the every 7-10d of 1/2MS nutrient solution, be beneficial to the growth of seedling.After sporophyte seedling grows 2 true leaves that about 20mm is high, seedling is planted in preprepared vinyl disc or foveola dish, water itself and matrix (3 parts of pure bog moss or bog mosses, 1 part of mixed preparing of perlite) are fully merged, transplanting survival rate is only 60% left and right.
Adopt same traditional seedling nursing device and method to carry out the seeding and seedling raising of other pteridophytes, transplanting process gametophyte and sporophyte are all subject to damage in various degree, the transplanting survival rate of spinulose tree fern is only 62%, the transplanting survival rate of white jade brake fern is only 75%, and the transplanting survival rate of cyrtomium caryotideumPresl is only 65%.
Method provided by the invention and existing methodical comparison:
(1) two kind of device sowing, occurs that at a large amount of heart-shaped prothallium early stage is without significant difference; Later stage is because gametophyte is grown up gradually, mutually crowded, and a large amount of water droplets that assemble at the seedling nursing device box top of conventional method are dropped in gametophyte surface, is easy to cause mouldy rotting, once infect, develops very soon, and whole seed tray all destroys; Seedling nursing device provided by the invention adopts water conservancy diversion and convection current ventilation device, adds the division of the little lattice of upper seeding wheel face, relatively independent, greatly reduces the occurrence probability of cross-infection;
(2) it is simple and easy to do that seedling nursing device provided by the invention divides the transplantation of seedlings stage, and cost of labor is low, and high effeciency has been avoided the impact (as reduced the damage to seedling, slow seedling is fast) of many human factors; The regularity of seedling is more consistent, has avoided seedling number how many, not of uniform size situation in every cave to occur;
(3) seedling nursing device provided by the invention is applicable to small-sized seed seeding and seedling raising very much.
Claims (2)
1. pteridophyte seedling nursing device, comprises seedling-growing container, sowing dividing plate and culture matrix release member;
The lid (3) that described seedling-growing container comprises basin body (2) and mates with it; Described basin body is the cavity being comprised of diapire and basin body sidewall, and diapire is provided with an above through hole; Described lid is the cavity being comprised of roof and covered body wall, and covered body wall is provided with the convection holes for Air Flow;
Described sowing dividing plate (4) is located in described basin body, for by dividing plate sidewall be located at the cavity body with some grids chamber that the grid sidewall in dividing plate sidewall forms;
Described culture matrix is released member (5) and is comprised base and be located on described base and the equal form fit in all grids described sowing dividing plate chamber and some convex columns one to one;
The diameter of described through hole is 3-6mm;
The diameter of described convection holes is 2-3mm;
Described basin body is rectangular-shaped, by diapire and four basin body sidewalls, formed, the length of the inner chamber of described basin body be 163mm, width be 153mm, highly for 59mm; Described lid is rectangular-shaped, by roof and four covered body walls, formed, the length of the inner chamber of described lid be 163mm, width be 153mm, highly for 29mm; On described diapire, be evenly provided with 8 through holes; Described covered body wall is provided with two groups of convection holes, and on each covered body wall one group, two groups of convection holes are symmetrical arranged, and every group of convection holes comprises 6 convection holes;
In every group of convection holes, in the middle of 1 convection holes is positioned at, it is around around other 5 convection holes;
Described sowing dividing plate is rectangular-shaped, and outer length is that 161mm, outer width are 151mm, and outer height is 15mm, has the grid chamber that 240 internal diameters are 9 * 9mm;
Described base is rectangular-shaped, and outer length is that 180mm, outer width are that 170mm, outer height are 5mm; On described base, distance edge 11mm to be to be provided with 240 rectangular-shaped convex columns in interior scope, and the external diameter of each convex column is 8 * 8mm, is highly 20mm, and the vertical range of every two convex column outers is 2mm;
Described pteridophyte seedling nursing device also comprises the pallet (1) for placing described seedling-growing container.
2. the application of pteridophyte seedling nursing device in pteridophyte is grown seedlings described in claim 1.
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| CN103814767A (en) * | 2014-02-07 | 2014-05-28 | 兰州大学 | Quick culturing device for bryophyte experimental materials |
| CN106613160A (en) * | 2016-11-23 | 2017-05-10 | 云南云投生态环境科技股份有限公司 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
| CN107637427A (en) * | 2017-09-22 | 2018-01-30 | 界首市永银种植专业合作社 | A kind of device for raising seedlings and method for culturing seedlings for growth of watermelon |
| CN109006439A (en) * | 2018-09-10 | 2018-12-18 | 北京昌回林海生态科技有限公司 | A kind of venushair fern sapling multiplication method based on hole tray live streaming spore method |
| CN112673947B (en) * | 2020-12-18 | 2022-09-09 | 青岛滕润翔检测评价有限公司 | Lily vegetable seed seedling raising device and seedling raising method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0380026A (en) * | 1989-08-22 | 1991-04-04 | Mametora Noki Kk | Method for growing soil-stick seedling for transplantation |
| CN2143870Y (en) * | 1992-11-19 | 1993-10-20 | 刘宏伟 | Nested soil-blocks tray |
| US5419080A (en) * | 1991-02-01 | 1995-05-30 | Gardener's Supply | Multi-celled tray for growing plants |
| CN2280411Y (en) * | 1997-03-04 | 1998-05-06 | 游义杰 | Soil-cultivation greenhouse |
| CN202635209U (en) * | 2012-05-07 | 2013-01-02 | 四川先拓生物科技有限公司 | Seedling raising culture device |
| CN203120554U (en) * | 2013-02-01 | 2013-08-14 | 中国科学院植物研究所 | Fern seedling raising device |
-
2013
- 2013-02-01 CN CN201310041580.4A patent/CN103081745B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0380026A (en) * | 1989-08-22 | 1991-04-04 | Mametora Noki Kk | Method for growing soil-stick seedling for transplantation |
| US5419080A (en) * | 1991-02-01 | 1995-05-30 | Gardener's Supply | Multi-celled tray for growing plants |
| CN2143870Y (en) * | 1992-11-19 | 1993-10-20 | 刘宏伟 | Nested soil-blocks tray |
| CN2280411Y (en) * | 1997-03-04 | 1998-05-06 | 游义杰 | Soil-cultivation greenhouse |
| CN202635209U (en) * | 2012-05-07 | 2013-01-02 | 四川先拓生物科技有限公司 | Seedling raising culture device |
| CN203120554U (en) * | 2013-02-01 | 2013-08-14 | 中国科学院植物研究所 | Fern seedling raising device |
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| Publication number | Publication date |
|---|---|
| CN103081745A (en) | 2013-05-08 |
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