CN103814767A - Quick culturing device for bryophyte experimental materials - Google Patents

Quick culturing device for bryophyte experimental materials Download PDF

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Publication number
CN103814767A
CN103814767A CN201410044939.8A CN201410044939A CN103814767A CN 103814767 A CN103814767 A CN 103814767A CN 201410044939 A CN201410044939 A CN 201410044939A CN 103814767 A CN103814767 A CN 103814767A
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China
Prior art keywords
bryophyte
upper cover
culture
setting
culturing device
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Pending
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CN201410044939.8A
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Chinese (zh)
Inventor
尚振艳
韩炳宏
牛得草
傅华
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Lanzhou University
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Lanzhou University
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Priority to CN201410044939.8A priority Critical patent/CN103814767A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The invention relates to a quick culturing device for massive homogeneous bryophyte experimental materials in a laboratory. The quick culturing device is composed of a lofting tank, an upper cover, a culturing tray support, a culturing tray and the like, the lofting tank and the upper cover thereof are connected by two hinges, and ventilation holes for plants to breathe are arranged on the left side and the right side of the upper cover; in order to prevent water vapor in the culturing device from losing too quickly, a culturing device sealing ring is designed to fix a buckling lock and a buckling ring of the upper cover and the lofting tank; in order to bring convenience to moving of the culturing device, handles are arranged on the culturing device. The quick culturing device can be used for studying of bryophyte developmental biology and bryophyte physiological ecology, and technical support can be provided for quick culturing of massive homogeneous experimental materials in the process of bryophyte experimental teaching in schools.

Description

Bryophyte experiment material fast culture device
Technical field
The present invention relates to a kind of device for a large amount of homogeneous bryophyte experiment material fast culture.
Background technology
Bryophyte blazons all over the world, all has distribution from polar region to the torrid zone.Bryophyte belongs to rudimentary plant, there is no the differentiation of real root, stem and leaf organ, can carry out photosynthesis, in botanic evolution process, representing the type that is transitioned into gradually Lu Sheng from aquatic, in the research of Developmental Biology, Chang Zuowei model plant material is added on research and utilization.In addition, bryophyte becomes the important pioneer in native evolutionary process as soil, can improve environment with metabolism by its life, improve the utilizability of soil nutrient, change soil physics structure and microflora feature, for higher plant settle down creation basic condition, be the focus of agrology research field concern so far to the improvement process of soil physical chemistry and biological property and mechanism and settle down rear about bryophyte.The research that develops into above-mentioned two scientific contents of experimental biology provides important thinking, and the fast culture of a large amount of homogeneous bryophyte materials under indoor conditions is the important foundation that guarantees that above-mentioned scientific research content is carried out smoothly.
So far, how to realize the fast culture of a large amount of homogeneous bryophyte materials in laboratory, become above-mentioned scientific research content at the indoor important bottleneck of carrying out analog study.The condition of bryophyte the most suitable growth is hot and humid environment, i.e. 25 ℃ of left and right of temperature, and relative air humidity reaches 80%.And at present about the cultivation of bryophyte experiment material, normally use for reference the cultural method in the artificial incubator of higher plant, and there is the culturing pot of liver moss trophosome to be positioned in 25 ℃ of incubators inoculation, give certain light, spray water repeatedly artificial every day.Field planting and the growth rate of the liver moss experiment material of this mode under cultivating are extremely slow, the bryophyte distributing take Long Zhong loess plateau, Gansu is as inoculation material is as example, conventionally need 50 days from being inoculated into 5 centimetres of left and right of trophosome growth on the ground, badly influence development of scientific research speed.The weak point of said method is because the common volume of incubator is larger, although the temperature inside the box can maintain 25 ℃ substantially, in case, humidity is restive more than 80%.In addition, the air humidity in incubator is obviously inhomogeneous, makes the liver moss experiment material growing way of cultivating under same treatment inconsistent.In addition, the cultivation of bryophyte material in enormous quantities, often needs very large space, in process of scientific research, is often subject to the restriction in incubator quantity and space, makes bryophyte experimental biology research under multiprocessing condition be difficult to carry out.
Than the condition of culture of higher plant, rudimentary plant liver moss is because biomass is few, and total oxygen demand is few, but higher for hot and humid culture environment conditional request, in addition, the hydrophily of bryophyte tissue own is stronger, therefore prior art be not suitable for the cultivation of bryophyte material.
Summary of the invention
The invention provides a kind of for a large amount of homogeneous bryophyte experiment material fast culture devices.
Bryophyte experiment material fast culture device of the present invention, it is characterized in that device is made up of setting-out groove, upper cover, culture tray support, culture tray, wherein: culture apparatus upper cover is prepared with transparent material, and the air-vent for plant respiration is left in the left and right sides on it, gapped between the inwall of culture tray and setting-out groove, the top edge of setting-out groove is provided with sealing ring, between setting-out groove and upper cover, be hinged, and setting-out groove and on cover and be respectively arranged with padlock and clasp.
In bryophyte experiment material fast culture device of the present invention, be also provided with and heat and lagging facility and humidification setting.
Bryophyte experiment material fast culture device of the present invention leaves the air-vent for plant respiration in the left and right sides of culture apparatus upper cover, simultaneously, for preventing that in culture apparatus, steam scatters and disappears too fast, design culture apparatus sealing ring and culture apparatus padlock and clasp, on culture apparatus of the present invention, be also provided with handle in its left and right sides respectively for convenience of mobile.The present invention has overcome the deficiencies in the prior art, adopt small size, flat cabinet design theory, to improve in culture apparatus air humidity as core, inoculation is had to as far as possible near apart from design of water level in culture tray and culture apparatus upper cover and the culture apparatus setting-out groove of liver moss trophosome, when making bryophyte material fully to absorb the steam in setting-out groove, the steam that culture apparatus upper cover condenses also can give bryophyte material and supplement more moisture.In addition, for further improving air humidity in culture apparatus, only be provided with a small amount of air-vent for plant respiration in culture apparatus upper cover both sides, simultaneously, between culture apparatus upper cover and culture apparatus setting-out groove, culture apparatus sealing shroud and culture apparatus padlock and clasp are designed, thereby both guaranteed the supply of oxygen in culture apparatus, prevented again that in culture apparatus, steam scattered and disappeared too fast.
Simple in structure, easy to use, with low cost, the good feature of cultivating, managing well, carrying well that the present invention has, can be used for the research of bryophyte Developmental Biology and bryophyte Physiological Ecology aspect, meanwhile, can provide technical support about the fast culture of a large amount of homogeneous experiment materials in the teaching Process of experiment of bryophyte for school.
When being provided with Heating system and humidification setting in device of the present invention, after electric heater unit and humidifier with attemperating unit, can make the application of device more convenient.
accompanying drawing explanation
The schematic diagram that accompanying drawing 1 is embodiments of the invention.In figure: 1 is culture apparatus upper cover; 2 is culture apparatus left side air-vent; 3 is culture apparatus sealing ring; 4 is bryophyte experiment material; 5 is culture apparatus left side handle; 6 culture apparatus setting-out grooves; 7 is culture tray left socle; 8 is culture apparatus padlock; 9 is culture tray medium-height trestle; 10 is culture tray right support; 11 is culture apparatus clasp; 12 is culture apparatus right side air-vent; 13 is the upper and lower connection hinge of culture apparatus; 14 is culture tray; 15 is culture apparatus right side handle; 16 is predetermined water level line in culture apparatus setting-out groove.
Embodiment
Following the present invention explains orally by reference to the accompanying drawings in detail.
From accompanying drawing, bryophyte experiment material fast culture device of the present invention is mainly by setting-out groove 6, the upper cover 1 that transparent material is made, for placing the left, center, right support 7,9,10 of culture tray, culture tray 14 forms, between the inwall of the edge of culture tray 14 and setting-out groove 1, leave gap, circulate for aqueous vapor.Between setting-out groove 1 and its upper cover, connected by two hinges 13.Leave the air- vent 2,12 for plant respiration in the left and right sides of culture apparatus upper cover, meanwhile, for preventing that in culture apparatus, steam is lost too fast, be provided with the sealing ring 3 made from flexible material at the top edge of setting-out groove.In addition, on setting-out groove 6 and upper cover 1, be respectively arranged with padlock (8) and clasp (11), for mutual snap close between upper cover 1 and setting-out groove 6 is fixed, make sealing ring realize sealing simultaneously, interaction between snap close can make the compressed distortion of sealing ring 3 in use, and the sealing forming between setting-out groove 6 and upper cover 1 causes humidity reduction in device in case the aqueous vapor in locking apparatus is overflowed.For convenience of mobile, putting handle 5,15 respectively in the device of the present invention left and right sides.
Occupation mode of the present invention is as follows: at the interior injection running water of culture apparatus setting-out groove 6 to predetermined water level line 16, there is the culture tray 14 of liver moss trophosome to be positioned on culture tray left, center, right support 7,9,10 inoculation, and send appropriate water (can not ponding) forth with watering can thereon, the culture apparatus upper cover 1 that closes, buckles culture apparatus padlock 8 and clasp 11.Then, bryophyte experiment material fast culture device being positioned over and being filled in air-conditioned laboratory, is 28 ℃ by Temperature Setting, under indoor illumination intensity, cultivates.Supplement week about culture apparatus setting-out trough inner water amount to predetermined water level line, meanwhile, have in inoculation in the culture tray of liver moss trophosome and to send appropriate water (can not ponding) forth with watering can.The utilization of multiple these culture apparatuses, just can realize a large amount of homogeneous bryophyte experiment material fast culture.
In bryophyte experiment material fast culture device, also can be provided with and heat and lagging facility and humidification setting, like this can more convenient use, but in accompanying drawing 1, do not represent this part content.

Claims (2)

1. bryophyte experiment material fast culture device, it is characterized in that device is by setting-out groove (6), upper cover (1), culture tray support (7), (9), (10), culture tray (14) forms, wherein: culture apparatus upper cover is prepared with transparent material, and the air-vent (2) for plant respiration is left in the left and right sides on it, (12), gapped between the inwall of culture tray (14) and setting-out groove (6), the top edge of setting-out groove is provided with sealing ring (3), between setting-out groove (6) and upper cover (1), connect with hinge (13), and on setting-out groove (6) and upper cover (1), be respectively arranged with padlock (8) and clasp (11).
2. bryophyte experiment material fast culture device according to claim 1, is characterized in that being also provided with and heating and lagging facility and humidification setting in device.
CN201410044939.8A 2014-02-07 2014-02-07 Quick culturing device for bryophyte experimental materials Pending CN103814767A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105462831A (en) * 2016-01-15 2016-04-06 贵州大学 Fruit disease pathogenic bacteria inversed grafting culture device

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1039188A (en) * 1964-07-31 1966-08-17 Frederick John Constable Improvements in or relating to propagators
WO2000018215A1 (en) * 1998-09-16 2000-04-06 Daled Ab Device for promoting growth from seed to plant
CN2419597Y (en) * 2000-05-19 2001-02-14 刘靖北 Large opening creature culturing container with flat annualar heat resisting rubber sealing
JP3559814B2 (en) * 2000-11-21 2004-09-02 独立行政法人 宇宙航空研究開発機構 Plant growth container
CN1559194A (en) * 2004-03-02 2005-01-05 广东省农业科学院花卉研究所 Method for breeding sprout by tissue culture, and vessels therefor
CN2800737Y (en) * 2005-06-10 2006-08-02 中国农业科学院农业环境与可持续发展研究所 Plant culturing box
EP2085463A1 (en) * 2008-02-01 2009-08-05 Eppendorf AG Culture plate comprising a lid for lateral ventilation
CN202285758U (en) * 2011-11-09 2012-07-04 叶军 Intelligent plant and vegetable culture box
CN102835310A (en) * 2012-05-12 2012-12-26 姜伟 Double-tray combined type plant tissue culture container
CN202635209U (en) * 2012-05-07 2013-01-02 四川先拓生物科技有限公司 Seedling raising culture device
CN202799829U (en) * 2012-04-30 2013-03-20 西南民族大学 Hydroponic grass planting box
CN103081745A (en) * 2013-02-01 2013-05-08 中国科学院植物研究所 Pteridophyte seedling raising device
KR101301470B1 (en) * 2011-07-11 2013-08-29 (주)모이라 Air cleaner using plant
CN203675730U (en) * 2014-02-07 2014-07-02 兰州大学 Bryophyte experimental material rapid culturing device

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1039188A (en) * 1964-07-31 1966-08-17 Frederick John Constable Improvements in or relating to propagators
WO2000018215A1 (en) * 1998-09-16 2000-04-06 Daled Ab Device for promoting growth from seed to plant
CN2419597Y (en) * 2000-05-19 2001-02-14 刘靖北 Large opening creature culturing container with flat annualar heat resisting rubber sealing
JP3559814B2 (en) * 2000-11-21 2004-09-02 独立行政法人 宇宙航空研究開発機構 Plant growth container
CN1559194A (en) * 2004-03-02 2005-01-05 广东省农业科学院花卉研究所 Method for breeding sprout by tissue culture, and vessels therefor
CN2800737Y (en) * 2005-06-10 2006-08-02 中国农业科学院农业环境与可持续发展研究所 Plant culturing box
EP2085463A1 (en) * 2008-02-01 2009-08-05 Eppendorf AG Culture plate comprising a lid for lateral ventilation
KR101301470B1 (en) * 2011-07-11 2013-08-29 (주)모이라 Air cleaner using plant
CN202285758U (en) * 2011-11-09 2012-07-04 叶军 Intelligent plant and vegetable culture box
CN202799829U (en) * 2012-04-30 2013-03-20 西南民族大学 Hydroponic grass planting box
CN202635209U (en) * 2012-05-07 2013-01-02 四川先拓生物科技有限公司 Seedling raising culture device
CN102835310A (en) * 2012-05-12 2012-12-26 姜伟 Double-tray combined type plant tissue culture container
CN103081745A (en) * 2013-02-01 2013-05-08 中国科学院植物研究所 Pteridophyte seedling raising device
CN203675730U (en) * 2014-02-07 2014-07-02 兰州大学 Bryophyte experimental material rapid culturing device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105462831A (en) * 2016-01-15 2016-04-06 贵州大学 Fruit disease pathogenic bacteria inversed grafting culture device

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Application publication date: 20140528