CN105462831A - Fruit disease pathogenic bacteria inversed grafting culture device - Google Patents

Fruit disease pathogenic bacteria inversed grafting culture device Download PDF

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Publication number
CN105462831A
CN105462831A CN201610025167.2A CN201610025167A CN105462831A CN 105462831 A CN105462831 A CN 105462831A CN 201610025167 A CN201610025167 A CN 201610025167A CN 105462831 A CN105462831 A CN 105462831A
Authority
CN
China
Prior art keywords
incubator
fruit
pathogenic bacteria
disease pathogenic
reversal connection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610025167.2A
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Chinese (zh)
Inventor
李明
龙友华
张承
吴小毛
邓广俊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guizhou University
Original Assignee
Guizhou University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guizhou University filed Critical Guizhou University
Priority to CN201610025167.2A priority Critical patent/CN105462831A/en
Publication of CN105462831A publication Critical patent/CN105462831A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

Abstract

The invention discloses a fruit disease pathogenic bacteria inversed grafting culture device. The device comprises an incubator(1) made of transparent materials, two movable incubator covers (2) are arranged at the top of the incubator(1), and the movable incubator covers (2) are matched with sliding grooves (4) formed in the upper portion of the incubator(1); a platform is arranged at the bottom of the interior of the incubator(1), and two or more rows of semicircular fruit carrying cups (8) which are different in size and specification are arranged on the platform in rows; a temperature and humidity regulator (5) is further arranged at one side of the interior of an incubator body of the incubator(1), the temperature and humidity regulator (5) is connected to the exterior of the incubator(1) through a connecting line (6), and a plug (7) is arranged at the end of the connecting line (6). Compared with the prior art, the device has the advantages of being controllable in temperature and humidity, large in culture capacity, capable of achieving aseptic technique and saving materials, simple in structure, wide in application prospect and the like.

Description

A kind of fruit disease pathogenic bacteria reversal connection culture apparatus
Technical field
The present invention relates to a kind of fruit disease pathogenic bacteria reversal connection incubator, belong to scientific experiment equipment technical field.
Background technology
Plant diseases ( plantdisease) refer to that plant is under the impact of biological or abiotic factor, the pathological change on a series of form, physiology and chemistry occurs, hinders the process of normal growth, growth, thus affect the phenomenon of human economy benefit.It can be divided into infectious disease and noninfectious disease two class according to the kind of cause of disease.And infectious disease is the disease that a class is caused by biotic factor, there is the features such as pathogenicity bo, infectivity, symptom specificity, the course of disease, Disease Cycle, usually cause the death such as farm crop and fruit tree, agricultural-food total crop failure; That includes the broad varietys such as fungal disease, bacterial disease, Disease, nematode venereal disease evil, parasitic seed plant disease.
And plant pathology is adopt Koch's Postulates when identifying a kind of unknown disease, that includes 1) on sick plant, be often attended by the existence of a kind of pathogenic micro-organism; 2) this microorganism can obtain pure culture in separation and purification in vitro or artificial medium; 3) pure culture is inoculated on the healthy tree of same breed, occurs the disease that symptom is identical; 4) from the plant of inoculation morbidity, be separated to its pure culture again, proterties is identical with inoculum; If carried out above-mentioned four step qualification work to obtain certain evidence, just can confirm that this microorganism is its pathogen.Above-mentioned 3rd) step plant pathology is often called " pathogenic bacteria reversal connection ", and in pathogenic bacteria reversal connection to plant after usually need could grow under specific habitat consequently to reach after reversal connection is fallen ill carry out the 4th) the qualification work of step.What specific habitat had the greatest impact is humiture, and should cultivate under a relative sterile environment after pathogenic bacteria reversal connection to plant, to reach the accuracy (controlled variable principle) affecting qualification from other pathogenic micro-organism dip-dye inoculation mouth.
Existing fruit germ reversal connection culture apparatus mainly beaker etc., usually have difficult controls of humiture, aseptic controlling nanmu, consumptive material greatly, the shortcoming such as complicated operation.
Summary of the invention
Advantages such as the object of the invention is the shortcoming for overcoming prior art and a kind of fruit disease pathogenic bacteria reversal connection culture apparatus is provided, it has, and humiture is controlled, cultivation amount is large, aseptic technique, saving material, structure are simple and have a extensive future.
Technical scheme of the present invention is such:
A kind of fruit disease pathogenic bacteria reversal connection culture apparatus, it comprises the incubator that a transparent material is made, and this transparent material is preferably transparent glass or plastics.The top of this incubator is provided with the mobile case lid of two fans, and mobile case lid coordinates with incubator top chute; Be provided with a platform at incubator inner bottom part, platform is in a row provided with the semicircle fruit hypanthium that the above size specification of two rows differs, fruit to be seeded is placed in fruit hypanthium, and fruit has pathogenic bacteria vaccination.The inner side of incubator box body is also provided with humiture control device, and humiture control device is connected to outside incubator by wire, and the termination of wire is provided with plug.The superimposed setting of the two mobile case lids of fan, and be that track carries out relatively sliding with chute.By the relatively sliding of mobile case lid, form a kind of two-layer adjustable cover plate.Movable case covers to be provided with and makes it easy to take chute as the handle that track carries out push-and-pull slip, and handle is rectangular parallelepiped bulge-structure.Artificial driving handle during operation, handle drives movable case lid to slide.
In addition, humiture control device being provided with switch, temperature regulator, humidity regulation device and indicating meter, for realizing the temperature and humidity regulation and control in incubator, and showing in real time.
Owing to have employed technique scheme, compared with prior art, the advantages such as the present invention has that humiture is controlled, cultivation amount is large, aseptic technique, saving material, structure are simple and have a extensive future
Accompanying drawing explanation
Fig. 1 is apparatus structure schematic diagram of the present invention.
Description of reference numerals: 1-incubator, 2-moves case lid, 3-handle, 4-chute, 5-humiture control device, 6-wire, 7-plug, 8-fruit hypanthium, 9-fruit.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.
As shown in Figure 1, the present invention is so a kind of fruit disease pathogenic bacteria reversal connection incubator, it comprises the incubator 1 that a transparent material is made as transparent glass or plastics, this incubator 1 be Specifically above that top is superimposed with each other and is provided with the mobile case lid 2 of two fans, mobile case lid 2 coordinates with the chute 4 as track of incubator 1 inside upper part, and mobile case lid 2 is provided with rectangular handle 3, be provided with at incubator 1 inner bottom part the semicircle fruit hypanthium 8 that size specification differs; Incubator 1 box house side is also provided with humiture control device 5, and humiture control device 5 is connected with wire 6, and wire 6 termination is provided with plug 7 for switching on power, and in figure, wire 6 is arranged according to spirrillum.This humiture control device 5 is provided with switch, temperature regulator, humidity regulation device and indicating meter.
Fruit hypanthium 8 size specification is multiple, quantity is adjustable.Fruit 9 is placed in fruit hypanthium 8, and fruit 9 has pathogenic bacteria vaccination.
During use, driving handle 3 makes mobile case lid 2 move along chute 4 and opens mobile case lid 2, the fruit 9 inoculated by pathogenic bacteria is placed in the fruit hypanthium 8 in incubator 1, then mobile case lid 2 is covered, the plug 7 of wire 6 termination is switched on power, the switch opening humiture control device 5 makes it in running order, according to the humiture state of indicating meter display, temperature regulator and humidity regulation device is regulated to cultivate.
Certainly, more than just embody rule example of the present invention, the technical scheme that the present invention also has other embodiment, all employings to be equal to replacement or equivalent transformation to be formed, all drops within protection domain of the presently claimed invention.

Claims (6)

1. a fruit disease pathogenic bacteria reversal connection culture apparatus, it is characterized in that: it comprises the incubator (1) that a transparent material is made, the top of this incubator (1) is provided with the mobile case lid of two fan (2), and mobile case lid (2) coordinates with incubator (1) top chute (4); Be provided with a platform at incubator (1) inner bottom part, platform be in a row provided with the semicircle fruit hypanthium (8) that the above size specification of two rows differs; Incubator (1) box house side is also provided with humiture control device (5), and humiture control device (5) is connected to incubator (1) outward by wire (6), and the termination of wire (6) is provided with plug (7).
2. fruit disease pathogenic bacteria reversal connection culture apparatus according to claim 1, is characterized in that: two fan mobile case lid (2) superimposed settings, and with chute (4) for track carries out relatively sliding.
3. fruit disease pathogenic bacteria reversal connection culture apparatus according to claim 1, is characterized in that: mobile case lid (2) is provided with and makes it easy to chute (4) for track carries out the handle (3) of push-and-pull slip, and handle (3) is rectangular parallelepiped bulge-structure.
4. fruit disease pathogenic bacteria reversal connection culture apparatus according to claim 1, is characterized in that: humiture control device (5) is provided with switch, temperature regulator, humidity regulation device and indicating meter.
5. fruit disease pathogenic bacteria reversal connection culture apparatus according to claim 1, is characterized in that: fruit (9) to be seeded is placed in fruit hypanthium (8), and fruit (9) has pathogenic bacteria vaccination.
6. fruit disease pathogenic bacteria reversal connection culture apparatus according to claim 1, is characterized in that: described transparent material is transparent glass or plastics.
CN201610025167.2A 2016-01-15 2016-01-15 Fruit disease pathogenic bacteria inversed grafting culture device Pending CN105462831A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610025167.2A CN105462831A (en) 2016-01-15 2016-01-15 Fruit disease pathogenic bacteria inversed grafting culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610025167.2A CN105462831A (en) 2016-01-15 2016-01-15 Fruit disease pathogenic bacteria inversed grafting culture device

Publications (1)

Publication Number Publication Date
CN105462831A true CN105462831A (en) 2016-04-06

Family

ID=55600993

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610025167.2A Pending CN105462831A (en) 2016-01-15 2016-01-15 Fruit disease pathogenic bacteria inversed grafting culture device

Country Status (1)

Country Link
CN (1) CN105462831A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103814767A (en) * 2014-02-07 2014-05-28 兰州大学 Quick culturing device for bryophyte experimental materials
CN204125462U (en) * 2014-08-22 2015-01-28 王新峰 Intellectual bacteriological incubator
CN204125464U (en) * 2014-05-11 2015-01-28 朱建疆 Cell culture incubator
CN204529856U (en) * 2015-04-15 2015-08-05 付玉华 A kind of anaerobic bacterium incubator
CN204888078U (en) * 2015-06-23 2015-12-23 申海防 Domestic fungus isolation meets seed case

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103814767A (en) * 2014-02-07 2014-05-28 兰州大学 Quick culturing device for bryophyte experimental materials
CN204125464U (en) * 2014-05-11 2015-01-28 朱建疆 Cell culture incubator
CN204125462U (en) * 2014-08-22 2015-01-28 王新峰 Intellectual bacteriological incubator
CN204529856U (en) * 2015-04-15 2015-08-05 付玉华 A kind of anaerobic bacterium incubator
CN204888078U (en) * 2015-06-23 2015-12-23 申海防 Domestic fungus isolation meets seed case

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Application publication date: 20160406