CN102498893A - Method for breeding cultivation seedlings of rare or endangered euryodendron excelsum artificially - Google Patents

Method for breeding cultivation seedlings of rare or endangered euryodendron excelsum artificially Download PDF

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Publication number
CN102498893A
CN102498893A CN2011103517139A CN201110351713A CN102498893A CN 102498893 A CN102498893 A CN 102498893A CN 2011103517139 A CN2011103517139 A CN 2011103517139A CN 201110351713 A CN201110351713 A CN 201110351713A CN 102498893 A CN102498893 A CN 102498893A
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seedling
seedlings
rare
seed
matrix
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CN102498893B (en
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王跃华
申仕康
蒋连计
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Yunnan University YNU
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Yunnan University YNU
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Abstract

The invention relates to a method for breeding cultivation seedlings of rare or endangered euryodendron excelsum artificially. The method comprises the following steps of: collecting mature euryodendron excelsum fruits in a species distribution area, and cleaning mature seeds; preparing a sprouting medium by using humus soil and loamy soil in a volume ratio of (1-3):1; mixing the seeds and dried river sand uniformly, broadcasting the mixture uniformly on a sprouting medium, covering a layer of surface humus soil, keeping the temperature of between 20 and 25 DEG C for 4 to 6 weeks to obtain seedlings with true leaves; after the seeds germinate, transplanting the seedlings with 2 to 5 true leaves, wherein the seedling transplanting medium is the same as the sprouting medium; and after transplanting the seedlings, ensuring moisture required by the growth in the seedling stage, allowing the seedlings in the environment of high temperature and high humidity to grow, and growing for 18 to 24 weeks to perform field transplantation. By the method, the seed germination rate of the euryodendron excelsum is over 70 percent, the survival rate of the seedlings is over 80 percent, the cultivation seedlings are robust, and 'little old seedlings' are avoided. The method is suitable for large-scale field transplantation. The method provides a technical guarantee for the salvation of the rare or endangered species, the recovery of population and the effective protection of resources.

Description

Rare or endangered species pig blood wood cultivation the Miaos worker mating system
Technical field
The invention belongs to biological technical field, be specifically related to rare or endangered species pig blood wood cultivation the Miaos worker mating system.
Background technology
Pig blood wood ( Euryodendron excelsumH. T. Chang) belong to Theaceae (Theaceae) pig blood wood and belong to the perennial woody plant, be the rare and endangered species of the peculiar monotypic genus of China.These species were set up by professor Zhang Hongda of Zhongshan University in 1963, and its phylogeny position is special, were the important materials that research Theaceae systematic growth and form develop, and simultaneously, pig blood ebon does straight and upright, and timber is extremely hard, is shipbuilding and architectural good timber.At present only in Guangdong Province spring county Ba Jia town have distribute and individual amount rare, field investigation finds that these species only deposit individual number 179 strains at present, plant 23 strains of growing up, and the distribution habitat influences seriously by artificial disturbance, natural population faces the danger of extinction.Therefore, these species have been classified as national secondary and have been laid special stress on protecting wild plant, are classified as critical species (critically endangered) by international nature with natural resources protection alliance (IUCN) simultaneously.
Pig blood wood is main with cross pollination, and flower yield is more, and the seed-setting amount is big, but the biennial bearing phenomenon is obvious, and because pig blood wood area is narrow, next-door neighbour village, distribution habitat receives local artificial serious interference, and population upgrades difficulty naturally.Yet; For this poky endangered species of pig blood wood, enlarging population quantity and carry out the species population back to nature through the artificial breeding seedling is to carry out its effectively important channel of protection, and in the process that adopts prior seed to grow seedlings, finds; Pig blood wood seed can normally be sprouted; The seedling lethality is very high but seedling builds up the phase, reaches 80% even more, and " little old man's seedling " phenomenon is serious.So traditional seedling culture method can not obtain the pig blood wood seedling of normal growth.
" little old man's seedling " just is meant the extremely slow or dormant seedling of growth.Obviously, this " little old man's seedling " can not be used as the transplanting seedling.Little old man's height of seedling of existing traditional seedling-cultivating method reaches 20-30%.
Summary of the invention
The objective of the invention is to propose a kind of method of rare or endangered species pig blood wood seedling artificial breeding; This method can reduce seedling significantly and build up phase seedling lethality; Avoid " little old man " seedling; For this rare plant kind in imminent danger of artificial cultivation provides high quality seedling, to overcome the deficiency of existing traditional seedling method.
This rare or endangered species pig blood wood cultivation the Miaos worker mating system that the present invention proposes is characterized in that it has the following steps:
(1) seed collection: collect ripe pig blood wood fruit on species distribution ground, clean mature seed;
(2) presprouting of seeds matrix is prepared: adopting humus soil and loam is the ratio of 1-3:1 according to volume ratio, configuration vernalization matrix;
(3) presprouting of seeds: after (1) step obtained seed and mix with dry river sand, uniform broadcasting on vernalization matrix covered one deck top layer humus soil behind the seed broadcasting, keep 20-25 ℃ of temperature, in time 4-6 week, obtains the long seedling that true leaf is arranged;
(4) seedling builds up and transplants: when treating behind the seed sprouting that seedling grows to 2-5 sheet true leaf; Transplant, seedling replanting matrix guarantees the required moisture of seedling growth with vernalization matrix behind the seedling replanting; And placing growth under the hot and humid environment, growth 18-24 can carry out the field transplanting after week.
The vernalization matrix humus soil in (2) step and loam are that 1:1 or 1:3 are good according to the ratio of stereometer.
It is standard that the top layer humus soil thickness in (3) step is not more than soil surface 1cm to guarantee to scatter seeds.
(3) step in, seed in vernalization matrix the degree of depth less than 2cm.
Said hot and humid environment of (4) step is more than 30 ℃ for the growth environmental temperature, below 40 [t1], and ambient humidity about in the of 80% 10 percentage points.
Concrete grammar of the present invention can reduce: get pig blood wood mature seed; Broadcast sowing in humus soil after evenly crumpling stirring with dry river sand: loam=1:1 (volume ratio) is to the vernalization matrix top layer of humus soil: loam=3:1 (volume ratio); The degree of depth is less than 2cm in vernalization matrix for seed, and presprouting of seeds is carried out in 20-25 ℃ of environment, when the back seedling of waiting to emerge grows to 2-5 sheet true leaf; Transplant to cultivation matrix (with vernalization matrix); Place hot and humid environment, promptly temperature is 30-40 ℃, grows in the environment of humidity about 80%.
Method of the present invention can be guaranteed the seeding ratio of pig blood wood more than 70%, and seedling percent reaches more than 80%, can obtain healthy and strong cultivation seedling 18-24 week, does not have " little old man's seedling ".Be suitable for large-scale open-air the transplanting.
Advantage of the present invention:
1, pig blood wood is one of plant the most rare and in imminent danger in the world, and in view of the mechanism in imminent danger of these species, enlarging population quantity through the seedling artificial breeding is effectively to protect the important channel of these species.The present invention cultivates pig blood wood seedling, to the development and use of conservation biology, RESTORATION ECOLOGY, population back to nature and the resource of these species important scientific basis and technological means is provided.
2, directly adopt pig blood wood seed rapidly and efficiently to carry out the artificial propagation of species seedling; Adopt the matrix of humus soil and loam combination, under hot and humid environment, breed, seedling percent is brought up to more than 80%; Improved more than 4 times than traditional seedling-cultivating method; And avoided the phenomenon of " little old man's seedling " fully, the cultivation seedling is healthy and strong, is suitable for extensive open-air the transplanting.For saving these rare and endangered species, being that the effective protection of repopulate and resource provides technical guarantee.
Description of drawings
Fig. 1 is that pig blood wood one-tenth seedling photo in kind alive is bred in manual work of the present invention.In the seedling age 18-24 week of the blood of pig shown in figure wood, robust growth, growing way are prosperous by force.
Fig. 2 is that pig blood wood one-tenth seedling photo in kind alive is bred in manual work of the present invention.Take in Yunnan University greenhouse on February 8th, 2010.
Embodiment
Face further specifies the present invention with instance, but scope of the present invention is not limited to the scope of instance.
Embodiment 1:
At the beginning of (1) 1 month pig blood wood ( Euryodendron excelsum) gather ripening fruits with distributing, rubbing is cleaned and obtained mature seed under running water, and is for use after drying naturally.
(2) adopt humus soil and red soil ratio (volume ratio) the configuration vernalization matrix according to 1:1, soil is faintly acid, vernalization matrix is placed the plastic crate vernalization of 60 * 42 * 30cm; Specific practice is: pad last layer filter course in plastic crate, and to spread then and mix vernalization matrix completely, the thickness of matrix in plastic crate is 10-12 centimetre; Water and irrigate matrix with thin shower nozzle; Smooth stromal surface then,, broadcast sowing in the vernalization stromal surface with pig blood wood mature seed and dry river sand mixing; Gently spread the humus soil that one deck sieves then, the humus soil thickness that broadcasts sowing is less than 1cm.The daily assurance soil moisture of watering, and place 20-25 ℃ greenhouse to carry out vernalization the plastic crate, in about 4-6 week, seeding ratio successively reaches 70%-80%.Sieving screen cloth humous here is 40 orders.
When (3) treating that seedling grows to 2-5 sheet true leaf, in the soil matrix with seedling replanting to humus soil: loam=1:1 (volume ratio) and humus soil: loam=3:1 (volume ratio), seedling replanting adopts plastic flowerpot to carry out; Every pot transplanting 4 strain seedling, 3 repetitions place the greenhouse to grow plastic flowerpot; The greenhouse condition remains on 30-40 ℃, and humidity about in the of 80% 10 percentage points is transplanted the back survival rate of seedling and reached 82%; Treat growth of seedling 18-24 week; About 40 centimetres of nursery stock plant heights, 0.8-1.2 centimetre in base footpath does not have damage by disease and insect.Can carry out the field by conventional method transplants.
Embodiment 2:
On routine 1 basis, change the vernalization substrate composition into humus soil and red soil according to 1:2, ratio (volume ratio), all the other are constant.Gained seedling replanting survival rate reaches 85%, becomes the seedling upgrowth situation as shown in Figure 1.
Instance 3:
On routine 1 basis, just the vernalization substrate composition changes humus soil and the red soil ratio (volume ratio) according to 1:3 into, and all the other are constant.Gained seedling replanting survival rate reaches 90%, becomes the seedling upgrowth situation as shown in Figure 2.

Claims (5)

1. rare or endangered species pig blood wood is cultivated the Miaos worker's mating system, it is characterized in that it has the following steps:
(1) seed collection: collect ripe pig blood wood fruit on species distribution ground, clean mature seed;
(2) presprouting of seeds matrix is prepared: adopting humus soil and loam is the ratio of 1-3:1 according to volume ratio, configuration vernalization matrix;
(3) presprouting of seeds: after (1) step obtained seed and mix with dry river sand, uniform broadcasting on vernalization matrix covered one deck top layer humus soil behind the seed broadcasting, keep 20-25 ℃ of temperature, in time 4-6 week, obtains the long seedling that true leaf is arranged;
(4) seedling builds up and transplants: when treating behind the seed sprouting that seedling grows to 2-5 sheet true leaf; Transplant, seedling replanting matrix guarantees the required moisture of seedling growth with vernalization matrix behind the seedling replanting; And placing growth under the hot and humid environment, growth 18-24 carries out the field transplanting after week.
2. according to the said rare or endangered species pig of claim 1 blood wood cultivation the Miaos worker mating system, vernalization matrix humus soil and the loam that it is characterized in that for (2) step is 1:1 or 1:3 according to the ratio of stereometer.
3. according to the said rare or endangered species pig of claim 1 blood wood cultivation the Miaos worker mating system, it is standard that the top layer humus soil thickness that it is characterized in that for (3) step is not more than soil surface 1cm to guarantee to scatter seeds.
4. according to claim 1 or 3 said rare or endangered species pig blood wood cultivation the Miaos worker mating systems, it is characterized in that seed in vernalization matrix the degree of depth less than 2cm.
5. according to the said rare or endangered species pig of claim 1 blood wood cultivation the Miaos worker mating system, it is characterized in that said hot and humid environment of (4) step is more than 30 ℃ for the growth environmental temperature, below 40 ℃, ambient humidity about in the of 80% 10 percentage points.
CN 201110351713 2011-11-09 2011-11-09 Method for breeding cultivation seedlings of rare or endangered euryodendron excelsum artificially Expired - Fee Related CN102498893B (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105918133A (en) * 2016-05-27 2016-09-07 陈思 Tissue culture and rapid propagation method of euryodendron excelsum
CN105993952A (en) * 2016-05-27 2016-10-12 陈思 Rapid breeding method of Euryodendron excelsum cultivation seedlings
CN109544429A (en) * 2018-12-27 2019-03-29 浙江大学城市学院 A method of Urban Biodiversity is improved using urban green space space child care endangered plants
CN110463525A (en) * 2019-09-19 2019-11-19 云南省林业科学院 A kind of method of minimum population wild plant near-earth protection
CN110809934A (en) * 2019-08-26 2020-02-21 中国科学院华南植物园 Method for germinating and raising seedlings of haematemesis suis seeds
CN112931015A (en) * 2021-03-23 2021-06-11 云龙县漕涧林场 Seedling raising technology for cinnamomum camphora seeds of minimum population plants
CN113068559A (en) * 2021-03-23 2021-07-06 苏州金螳螂园林绿化景观有限公司 Rapid seedling raising method for small seeds of cajeput

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
王跃华等: "山茶科濒危植物猪血木的生态与繁殖特性", 《云南植物研究》 *
申仕康等: "中国特有珍稀濒危植物猪血木的研究进展", 《中国野生植物资源》 *
申仕康等: "濒危植物猪血木(Euryodendron excelsum H.T.Chang)自然种群结构及动态", 《生态学报》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105918133A (en) * 2016-05-27 2016-09-07 陈思 Tissue culture and rapid propagation method of euryodendron excelsum
CN105993952A (en) * 2016-05-27 2016-10-12 陈思 Rapid breeding method of Euryodendron excelsum cultivation seedlings
CN105993952B (en) * 2016-05-27 2018-07-03 陈思 Pig blood wood cultivates seedling fast breeding method
CN105918133B (en) * 2016-05-27 2018-07-03 陈思 The quick mating system of pig blood wood tissue cultures
CN109544429A (en) * 2018-12-27 2019-03-29 浙江大学城市学院 A method of Urban Biodiversity is improved using urban green space space child care endangered plants
CN110809934A (en) * 2019-08-26 2020-02-21 中国科学院华南植物园 Method for germinating and raising seedlings of haematemesis suis seeds
CN110463525A (en) * 2019-09-19 2019-11-19 云南省林业科学院 A kind of method of minimum population wild plant near-earth protection
CN112931015A (en) * 2021-03-23 2021-06-11 云龙县漕涧林场 Seedling raising technology for cinnamomum camphora seeds of minimum population plants
CN113068559A (en) * 2021-03-23 2021-07-06 苏州金螳螂园林绿化景观有限公司 Rapid seedling raising method for small seeds of cajeput

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