CN101695249B - Non-tube rapid propagation method for dendrobium devonianum - Google Patents
Non-tube rapid propagation method for dendrobium devonianum Download PDFInfo
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- CN101695249B CN101695249B CN2009101912136A CN200910191213A CN101695249B CN 101695249 B CN101695249 B CN 101695249B CN 2009101912136 A CN2009101912136 A CN 2009101912136A CN 200910191213 A CN200910191213 A CN 200910191213A CN 101695249 B CN101695249 B CN 101695249B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a non-tube rapid propagation method for dendrobium devonianum. The method mainly comprises the following steps: constructing a seedbed; cultivating sound seedlings of maternal plants; treating stems before cuttage; planting the treated stems on the seedbed; and carrying out field management and seedling transplant. Due to carrying out plant non-tube rapid propagation according to the characteristics of dendrobium devonianum such as longer stems and more eustipes, the method has the advantages of low cost, quick speed and high transplant survival rate and is also a novel seedling rapid propagation approach of dendrobium plants. Moreover, the method solves the problems of the prior dendrobium tissue culture technology seriously restricting industrialization process such as great difficulty, high cost and low transplant survival rate.
Description
Technical field
The present invention relates to a kind of asexual reproduction method of the stem of noble dendrobium, relate to a kind of non-tube rapid propagation method for dendrobium devonianum in particular.
Technical background
Dendrobium devonianum (Dendrobium devonianum Paxt.) is the medicinal good merchantable brand of Application and Development in recent years, is commonly called as purple dendrobium, purple skin orchid, the Xiao Huang grass, bracketplant etc. mainly are distributed in the province such as Yunnan, Guangxi, Guizhou of China, and Burma, Vietnam, Laos, Thailand are also more.Because the market of dendrobium devonianum is better in recent years, the medicinal herb grower has carried out the harvesting of " predation formula " to dendrobium devonianum, make stem of noble dendrobium resource suffer considerable damage, domestic resource has been in Critical Condition, and the required dendrobium devonianum of its commodity mainly comes from domestic outlying mountain area and surrounding countries.
Chinese patent (ZL02134754.9) discloses a kind of method for quickly breeding of HERBA DENDROBII, mainly increases after prior art five steps " inducing clumping bud " step " adventitious buds proliferation cultivation ".China (ZL200410077729.5) discloses the tissue culture propagation of multiple autumn dendrobium original seed and hybrid; Chinese patent application (200710066480.1) discloses a kind of seedling-cultivating method of dendrobium candidum stem apex quick breeding by group culture; Chinese patent application (200710160571.1) discloses a kind of tissue cultivation rapid breeding method of spring dendrobium stem.Can lack problem to a certain degree solving the seedling that stem of noble dendrobium industry present stage faced by tissue culture quick breeding, but exist cost higher, technology difficulty is difficult to problems such as popularization greatly.
Chinese patent application (200810021790.6) discloses a kind of cottage breeding method of dendrobium candidum, but cuttage need consume a large amount of stem of noble dendrobium plant, and cost is higher, and the large-scale promotion difficulty is big.
According to statistics, the price of dendrobium devonianum linearly rises at present, and the vegetative propagation technique of research and popularization dendrobium devonianum will play the effect of protection dendrobium devonianum in imminent danger.
Summary of the invention
The invention provides a kind of non-tube rapid propagation method for dendrobium devonianum, non-test tube rapid propagation may further comprise the steps:
1) utilize the flaw-piece waste material of Wood Processing or asbestos shingle to do the end and make up the seedbed, with wood sawdust, broken bark, perlite as matrix;
2) the kind strain of stem section is carried out hormone regulating and controlling and fertilizer treatment at vigorous period and growth middle and later periods respectively;
3) the stem section is carried out the shaping of stem section, wound preliminary treatment and drying and other treatment before cuttage;
4) pretreated stem section is placed on the seedbed, the stem section is carried out half dewiness cover;
When 5) young shoot grows 1～2cm, under the condition of controlled humidity, spray NAA and nutrient solution;
6) plant transplantation of seedlings.
The height of bed 10～the 15cm in the described seedbed of step 1), bedside 1～1.2m, the high 0.8～1.0m of support; Thickness 5～the 10cm of matrix.
Step 2) described vigorous period hormone is controlled to be: in 6～September of the previous year of stem segment cuttage the maternal plant of stem section being sprayed GA1 concentration is the mixed liquor twice of 0.05～0.3mg/L for 1000mg/L+6-BA concentration, each 1 month at interval;
Growth middle and later periods fertilizer treatment is: at spraying concentration 0.01～0.1% nitrogenous fertilizer 3 times in 8～December of the previous year of stem segment cuttage, 20～30 days at interval, reduce using of phosphorus potash fertilizer simultaneously.
The time of the shaping of the described beginning stem of step 3) section, wound preliminary treatment and drying and other treatment is December to cuttage March then of cuttage the previous year;
The described stem section of step 3) is shaped as the kind stem of cutting fallen leaves, dissects to be every section stem section with a stipes in stipes;
The wound preliminary treatment allows cutting plane fully be covered for using ash in mix stirring with the stem section;
Drying and other treatment is for being 5～25 ℃ in temperature, intensity of illumination 1000～5000lx, condition under shone 3～5 days, after to be positioned over the ventilation air-dry to cuttage 4～June then.
Preliminary treatment is that the 6-BA of stem section with 0.05～0.3mg/L handled 1～3 hour;
The specification of putting is put the stem section for interval 3～8cm, and every mu in 10～150,000 rhizome sections;
The material that half dewiness covers is the grass meal or the sandy soil of wood sawdust, pulverizing.
The condition of the described humidity of step 5) is 40～80%;
Spray spraying concentration 0.1～0.8mg/LNAA once; Spray nutrient solution, the whole growth phase, every interval sprayed once in 20 days.
The time of described kind of transplantation of seedlings of step 6) is stem segment cuttage 2～April of next year.
Useful technique effect of the present invention is: the present invention is long according to the dendrobium devonianum stem, the more characteristic of stipes, carries out the non-tube rapid propagation of plant, than other cultivation methods such as tube rapid propagation and cuttages, saves cultivation and surpasses 50% with the stem section, saves work and surpasses 40%; Being the method that a kind of cost is low, speed is fast, transplanting survival rate is high, also is a kind of new quick reproduction technique approach of Dendrobium plant.
Embodiment
A, set up greenhouse and seedbed
The greenhouse of simulated tissue culture test tube microenvironment should be provided with the atomizing mini sprinkler and the light filling lamp of spray cooling in the greenhouse; Add a cover mocromembrane on the bow member and form shed integral body; Utilize the flaw-piece waste material or the asbestos shingle of Wood Processing to do the end, make the height of bed 10～15cm, bedside 1～1.2m, the bed long because of ground is decided, the implant bed of support 0.8～1.0m.Mix as matrix with wood sawdust, broken bark, perlite or by proper proportion; Matrix is laid on the seedbed thickness 5～10cm.
B, the stripped material of preparation
In 6～September of the previous year of stem segment cuttage the maternal plant of stem section is sprayed GA1 1000mg/L+6-BA 0.05～0.3mg/L twice, each 1 month at interval; Growth middle and later periods strong seedling cultivation is: at spraying concentration 0.01～0.1% nitrogenous fertilizer 3 times in 8～December of the previous year of stem segment cuttage, 20～30 days at interval, reduce using of phosphorus potash fertilizer simultaneously.
The time of the shaping of stem section, wound preliminary treatment and drying and other treatment is December to cuttage March then of cuttage the previous year; The stem section is shaped as the kind stem of cutting fallen leaves, dissects to be every section stem section with a stipes in stipes; The wound preliminary treatment is stirred for mixing in the stem section with ash, allows dissect fully covered; Drying and other treatment is for being 5～25 ℃ in temperature, intensity of illumination 1000～5000lx, condition under shone 3～5 days, after be positioned over that the ventilation is air-dry to be used to cuttage reserve in 4～June then.
C, seedling are cultivated
6-BA with 0.05～0.3mg/L handled 1～3 hour with the stem section; The specification of putting is put the stem section for 3～8cm at interval, and every mu in 10～150,000 rhizome sections, and the material that half dewiness covers is the grass meal or the sandy soil of wood sawdust, pulverizing, and the condition of humidity is 40～80%, sprays nutrient solution and be spraying concentration 0.1～0.8mg/LNAA once.Per 20 days of whole growth phase sprayed once.
Seedling was transplanted 2～April of next year.
Claims (2)
1. non-tube rapid propagation method for dendrobium devonianum, it is characterized in that: non-test tube rapid propagation may further comprise the steps:
1) utilize the flaw-piece waste material of Wood Processing or asbestos shingle to do the end and make up the seedbed, with wood sawdust, broken bark, perlite as matrix;
2) the kind strain of stem section is carried out hormone regulating and controlling and fertilizer treatment at vigorous period and growth middle and later periods respectively;
3) the stem section is carried out the shaping of stem section, wound preliminary treatment and drying and other treatment before cuttage;
4) pretreated stem section is placed on the seedbed, the stem section is carried out half dewiness cover;
When 5) young shoot grows 1～2cm, under the condition of controlled humidity, spray NAA and nutrient solution;
6) plant transplantation of seedlings;
Wherein, step 2) described vigorous period hormone is controlled to be: in 6～September of the previous year of stem segment cuttage the maternal plant of stem section being sprayed GA1 concentration is the mixed liquor twice of 0.05～0.3mg/L for 1000mg/L+6-BA concentration, each 1 month at interval;
Growth middle and later periods fertilizer treatment is: at spraying concentration 0.01～0.1% nitrogenous fertilizer 3 times in 8～December of the previous year of stem segment cuttage, 20～30 days at interval, reduce using of phosphorus potash fertilizer simultaneously;
The time of the shaping of the described beginning stem of step 3) section, wound preliminary treatment and drying and other treatment is December to cuttage March then of cuttage the previous year;
The described stem section of step 3) is shaped as the kind stem of cutting fallen leaves, dissects to be every section stem section with a stipes in stipes; The wound preliminary treatment allows cutting plane fully be covered for using ash in mix stirring with the stem section; Drying and other treatment was shone under the condition of intensity of illumination 1000～5000lx 3～5 days for being 5～25 ℃ in temperature, after to be positioned over the ventilation air-dry to cuttage 4～June then;
Preliminary treatment is that the 6-BA of stem section with 0.05～0.3mg/L handled 1～3 hour; The specification of putting is put the stem section for interval 3～8cm, and every mu in 10～150,000 rhizome sections; The material that half dewiness covers is the grass meal or the sandy soil of wood sawdust, pulverizing;
The condition of the described humidity of step 5) is 40～80%; Spray spraying concentration 0.1～0.8mg/LNAA once; Spray nutrient solution, the whole growth phase, every interval sprayed once in 20 days;
The time of described kind of transplantation of seedlings of step 6) is stem segment cuttage 2～April of next year.
2. non-tube rapid propagation method for dendrobium devonianum according to claim 1 is characterized in that: the height of bed 10～15cm in the described seedbed of step 1), bedside 1～1.2m, the high 0.8～1.0m of support; Thickness 5～the 10cm of matrix.
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| CN2009101912136A CN101695249B (en) | 2009-10-26 | 2009-10-26 | Non-tube rapid propagation method for dendrobium devonianum |
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| CN101695249B true CN101695249B (en) | 2011-05-04 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101999307B (en) * | 2010-10-21 | 2013-07-03 | 重庆市中药研究院 | Natural reproduction method for sowing dendrobium |
| CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技（上海）有限公司 | Method for natural propagation of dendrobium officinale seeds |
| CN102584431B (en) * | 2012-02-02 | 2014-02-12 | 云南腾众新能源科技有限公司 | Compound plant ash dendrobium seedling culture medium material and preparation method and application thereof |
| CN102972267A (en) * | 2012-11-26 | 2013-03-20 | 广东省农业科学院作物研究所 | Cuttage seedling method for dendrobium candidum |
| CN104094767A (en) * | 2014-07-31 | 2014-10-15 | 张松波 | Dendrobium officinale planting method |
| CN106106103B (en) * | 2016-07-11 | 2019-07-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch dendrobium nobile old stem cuttage breeding method |
| CN106857148A (en) * | 2017-02-22 | 2017-06-20 | 南京林业大学 | A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training |
| CN108770668A (en) * | 2018-06-22 | 2018-11-09 | 遵义市播州区惠联生态农业发展有限公司 | A kind of purple dendrobium capsule seed seedling-raising technique |
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Non-Patent Citations (3)
| Title |
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| 孙永玉等.齿瓣石斛栽培基质筛选及其栽培方式研究.《林业科学研究》.2007,第20卷(第4期),506-509. * |
| 李军萍等.齿瓣石斛种子培养及扩繁技术研究.《河北农业科学》.2008,第12卷(第7期),52-53. * |
| 郑志新等.云南龙陵齿瓣石斛化学成分分析测定及栽培方式选择.《安徽农业科学》.2008,第36卷(第4期),1426-1427. * |
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