CN101263787A - Louis iris tissue culture fast seedling establishment and ecological application method - Google Patents
Louis iris tissue culture fast seedling establishment and ecological application method Download PDFInfo
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- CN101263787A CN101263787A CNA2008100610558A CN200810061055A CN101263787A CN 101263787 A CN101263787 A CN 101263787A CN A2008100610558 A CNA2008100610558 A CN A2008100610558A CN 200810061055 A CN200810061055 A CN 200810061055A CN 101263787 A CN101263787 A CN 101263787A
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Abstract
The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.
Description
Technical field
The present invention relates to quick breeding and the ecological method of using of a kind of water plants, particularly the fast rapid-result seedling of Louis's iris and the ecological method of using.
Background technology
Louis's iris under field conditions (factors), based on plant division or seminal propagation, though division propagation is simple, single plant 1 year can only plant division once, reproduction rate generally has only 10 times, and is lower, is unfavorable for a large amount of breedings, it is longer to be applied to the ecological cycle.
Though most of iris kind can reach the purpose of quick breeding by the tissue culture mode, conventional tissue culture is used the cycle from the test-tube plantlet to ecology long, and survival rate can not get assurance.
Summary of the invention
Technical problem to be solved by this invention is that the above-mentioned defective that overcomes prior art provides fast rapid-result seedling of a kind of iris and the ecological method of using, and makes it can effectively fast rapid-result seedling and be effectively applied to ecology.
The present invention solves the problems of the technologies described above the technical scheme that is adopted:
Louis iris tissue culture fast seedling establishment and the ecological method of using may further comprise the steps:
1) the choosing and sterilizing of material: get the some strains of Louis's iris, clean up, culture in clear water, spend 2～3 days and change water one time, be colonizated in again in the nutrient solution 7 days, and got nutrition organs such as flower, bud, floral axis, blade and piece root, inoculate behind the routine disinfection as explant;
2) inducing and enrichment culture of bud: with 1) the nutrition organs of sterilizing be inoculated on the inducing culture, treat that callus is formed up to 1～2cm
2Size, change differential medium over to when having the green protruding block of 0.5～0.8cm, induce with differential medium and be all MS+6-BA2.5～3.5mgL
-1+ 2,4-D0.8～1.2mgL
-1, when root system grows to the 0.5cm left and right sides, change root media over to;
3) root induces
The consistent no offspring of choosing growth changes the inducing culture of root, medium MS+IBA 0.40～0.8mgL over to
-1Perhaps MS+BA0.10～0.30mgL
-1+ NAA 0.40～0.60mgL
-1Perhaps 1/2MS+BA0.10～0.30mgL
-1+ NAA0.40～0.60mgL
-1, when test-tube plantlet grows to 12～15cm, carry out hardening;
Above bud and root tissue culture condition are: 23 ± 2 ℃ of temperature, intensity of illumination 2000lx, light application time 11～12h/d;
4) hardening: emerge after 6～8 days at the natural lighting lower refining seedling; Wash test-tube plantlet root medium off, be transplanted in the container for plant growth that mixed-matrix is housed of sterilization, water, shade and be incubated, when seedling grows to 20cm length, directly go out the garden and be applied to ecology.
Above-mentioned steps 1) in the selection of explant, with the success easily of floral organs such as flower, bud, floral axis, especially with floral axis the best.
Above-mentioned steps 3) stage of taking root in shortens the time that test-tube plantlet is cultivated as long as root system also can enter the hardening stage when growing to the 0.5cm left and right sides.
Above-mentioned steps 4) container for plant growth in can be selected according to actual needs, as be used for the greening of water body chinampa formula can be with the control root container of taking root, the container size diameter is 10～20cm, highly is 15～35cm; As be directly used in and be colonizated in the earth the available non-woven fabric net bag of greening, the container size diameter is 5～10cm, highly is 10～15cm.Utilize above-mentioned these special seedling containers that fill to cultivate, the seedling growth is fast, and quality is good.
Above-mentioned used mixed-matrix is the mixture of peat+perlite+vermiculite+slow-release compound fertilizer, can promote the growth of seedling.
Compared with prior art, the invention has the advantages that:
1, directly is colonizated in the container of being furnished with mixed-matrix after the employing tissue culturing seedling hardening, the one-tenth seedling is fast, quality good, the cycle that seedling is cultivated shortens, and can directly apply in the construction of ecotopes such as wetland, waters, reduces conventional organization and cultivates the intermediate link that cultivate in the seedling land for growing field crops.
2, by tissue culture, its reproduction coefficient height can be bred in the anniversary, is fit to factorial seedling growth.
3, above-mentioned tissue culture is that advanced persons' such as genetic transformation, haploid breeding seedling growing process provides the foundation and condition, has shortened the time of breeding greatly.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1:
Louis iris tissue culture fast seedling establishment and the ecological method of using may further comprise the steps:
1, material choosing and sterilizing
Get the some strains of Louis's iris in the field, after bringing back the laboratory mud on its bulb is cleaned up, breed is in clear water, spend 2～3 days and change water one time, be colonizated in again in the nutrient solution of human configuration 7 days, get nutrition organs such as flower, bud, floral axis, blade and piece root as explant, inoculate behind the routine disinfection.
2, bud induces and enrichment culture
The nutrition organs such as flower, bud, floral axis, blade and piece root of sterilizing are inoculated on the inducing culture, treat that callus is formed up to 1～2cm
2Size, change differential medium over to when having the green protruding block of 0.5～0.8cm, about 30～35 days of time, induce with differential medium and be all MS+6-BA2.5～3.5mgL
-1+ 2,4-D0.8～1.2mgL
-1After cultivation in 10～15 days, when root system grows to about 0.5cm, no offspring changes root media over to when growing to 5～8cm length.
No offspring also can continue to change differential medium over to be broken up.
3, root induces
The consistent no offspring of choosing growth changes the inducing culture of root, medium MS+IBA 0.40～0.8mgL over to
-1Perhaps MS+BA0.10～0.30mgL
-1+ NAA 0.40～0.60mgL
-1Perhaps 1/2MS+BA0.10～0.30mgL
-1+ NAA0.40～0.60mgL
-1Begin to take root through 10～15 days cultivations, when test-tube plantlet grows to 12～15cm, carry out hardening.
Above bud and root tissue culture condition are: 23 ± 2 ℃ of temperature, intensity of illumination 2000lx, light application time 11～12h/d;
4, hardening
Emerge after 6～8 days at the natural lighting lower refining seedling.
Wash test-tube plantlet root medium off, be transplanted in the control root container in the mixed-matrix of peat+perlite+vermiculite+slow-release compound fertilizer of sterilization, water, shade and be incubated, survival rate about 95%.Conventional rich water quality management directly goes out the garden and is applied to ecology when seedling grows to 20cm length.
Above-mentioned container for plant growth can be selected according to actual needs, as be used for the greening of water body chinampa formula can be with the control root container of taking root, the container size diameter is 10～20cm, highly is 15～35cm; As be directly used in and be colonizated in the earth the available non-woven fabric net bag of greening, the container size diameter is 5～10cm, highly is 10～15cm.Utilize above-mentioned these special seedling containers that fill to cultivate, the seedling growth is fast, and quality is good.
Claims (5)
1, Louis iris tissue culture fast seedling establishment and the ecological method of using is characterized in that: may further comprise the steps:
1) the choosing and sterilizing of material: get the some strains of Louis's iris, clean up, culture in clear water, spend 2～3 days and change water one time, be colonizated in again in the nutrient solution 7 days, and got nutrition organs such as flower, bud, floral axis, blade and piece root, inoculate behind the routine disinfection as explant;
2) inducing and enrichment culture of bud: with 1) the nutrition organs of sterilizing be inoculated on the inducing culture, treat that callus is formed up to 1～2cm
2Size, change differential medium over to when having the green protruding block of 0.5～0.8cm, induce with differential medium and be all MS+6-BA2.5～3.5mgL
-1+ 2,4-D0.8～1.2 mgL
-1, when root system grows to the 0.5cm left and right sides, change root media over to;
3) root induces
The consistent no offspring of choosing growth changes the inducing culture of root, medium MS+IBA 0.40～0.8mgL over to
-1Perhaps MS+BA0.10～0.30mgL
-1+ NAA 0.40～0.60mgL
-1Perhaps 1/2MS+BA0.10～0.30mgL
-1+ NAA0.40～0.60mgL
-1, when test-tube plantlet grows to 12～15cm, carry out hardening;
Above bud and root tissue culture condition are: 23 ± 2 ℃ of temperature, intensity of illumination 2000lx, light application time 11～12h/d;
4) hardening: emerge after 6～8 days at the natural lighting lower refining seedling; Wash test-tube plantlet root medium off, be transplanted in the container for plant growth that mixed-matrix is housed of sterilization, water, shade and be incubated, when seedling grows to 20cm length, directly go out the garden and be applied to ecology.
2, Louis iris tissue culture fast seedling establishment as claimed in claim 1 and the ecological method of using, it is characterized in that: the step 1) explant is a floral axis.
3, Louis iris tissue culture fast seedling establishment as claimed in claim 1 or 2 and the ecological method of using, it is characterized in that: used mixed-matrix is the mixture of peat+perlite+vermiculite+slow-release compound fertilizer in the step 4).
4, Louis iris tissue culture fast seedling establishment as claimed in claim 1 or 2 and the ecological method of using, it is characterized in that: the container for plant growth in the step 4) is the container of taking root, the container size diameter is 10～20cm, highly is 15～35cm; Or the non-woven fabric net bag, the container size diameter is 5～10cm, highly is 10～15cm.
5, Louis iris tissue culture fast seedling establishment as claimed in claim 3 and the ecological method of using is characterized in that: the container for plant growth in the step 4) is the control root container of taking root, and the container size diameter is 10～20cm, highly is 15～35cm; Or the non-woven fabric net bag, the container size diameter is 5～10cm, highly is 10～15cm.
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| CNA2008100610558A CN101263787A (en) | 2008-05-08 | 2008-05-08 | Louis iris tissue culture fast seedling establishment and ecological application method |
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| CNA2008100610558A CN101263787A (en) | 2008-05-08 | 2008-05-08 | Louis iris tissue culture fast seedling establishment and ecological application method |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816287A (en) * | 2010-05-05 | 2010-09-01 | 南京大学 | Method for culturing Iris pseudacorus callus |
| CN102165920A (en) * | 2011-02-21 | 2011-08-31 | 浙江省萧山棉麻研究所 | Tissue culturing method for regenerating somatic cells of aquatic iris |
| CN101869072B (en) * | 2009-04-24 | 2012-01-04 | 上海上房园林植物研究所 | Tissue culture method of golden-heart Iris pseudacorus |
| CN102893872A (en) * | 2012-11-03 | 2013-01-30 | 云南省农业科学院花卉研究所 | Tissue culture method for domesticated seedlings of iris pallida |
| CN104429963A (en) * | 2014-12-08 | 2015-03-25 | 邱林 | Free pollen cultivation method of iris ensata thunb |
| CN104839019A (en) * | 2015-04-29 | 2015-08-19 | 浙江农林大学 | Method for in-vitro rapid propagation of iris laevigata by using immature fruits |
| CN104996302A (en) * | 2015-08-20 | 2015-10-28 | 浙江大学 | Method for remarkably improving callus inductivity of iris pseudacorus L. |
| CN106106178A (en) * | 2016-07-28 | 2016-11-16 | 北京林业大学 | A kind of method for tissue culture of confection Rhizoma Iridis Tectori |
| CN107494267A (en) * | 2017-09-27 | 2017-12-22 | 江苏农林职业技术学院 | A kind of cultural method of Iris germanica callus |
| CN108496800A (en) * | 2018-04-10 | 2018-09-07 | 黑龙江省科学院自然与生态研究所 | Chinese small iris high frequency regenerating system method is established based on callus induction |
| CN111567401A (en) * | 2020-04-20 | 2020-08-25 | 上海植物园 | Tissue culture rapid propagation method of butterfly flower' Mengdian |
| CN111887152A (en) * | 2019-11-19 | 2020-11-06 | 东北林业大学 | Method for establishing plant regeneration system of swallow flower bud growing way |
| CN114403000A (en) * | 2021-12-31 | 2022-04-29 | 湖北懿昌能兴生态农业科技有限公司 | Louisiana iris tissue culture propagation method with high plant regeneration rate |
-
2008
- 2008-05-08 CN CNA2008100610558A patent/CN101263787A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869072B (en) * | 2009-04-24 | 2012-01-04 | 上海上房园林植物研究所 | Tissue culture method of golden-heart Iris pseudacorus |
| CN101816287A (en) * | 2010-05-05 | 2010-09-01 | 南京大学 | Method for culturing Iris pseudacorus callus |
| CN102165920A (en) * | 2011-02-21 | 2011-08-31 | 浙江省萧山棉麻研究所 | Tissue culturing method for regenerating somatic cells of aquatic iris |
| CN102165920B (en) * | 2011-02-21 | 2012-11-14 | 浙江省萧山棉麻研究所 | Tissue culturing method for regenerating somatic cells of aquatic iris |
| CN102893872A (en) * | 2012-11-03 | 2013-01-30 | 云南省农业科学院花卉研究所 | Tissue culture method for domesticated seedlings of iris pallida |
| CN104429963A (en) * | 2014-12-08 | 2015-03-25 | 邱林 | Free pollen cultivation method of iris ensata thunb |
| CN104839019A (en) * | 2015-04-29 | 2015-08-19 | 浙江农林大学 | Method for in-vitro rapid propagation of iris laevigata by using immature fruits |
| CN104996302A (en) * | 2015-08-20 | 2015-10-28 | 浙江大学 | Method for remarkably improving callus inductivity of iris pseudacorus L. |
| CN106106178A (en) * | 2016-07-28 | 2016-11-16 | 北京林业大学 | A kind of method for tissue culture of confection Rhizoma Iridis Tectori |
| CN107494267A (en) * | 2017-09-27 | 2017-12-22 | 江苏农林职业技术学院 | A kind of cultural method of Iris germanica callus |
| CN108496800A (en) * | 2018-04-10 | 2018-09-07 | 黑龙江省科学院自然与生态研究所 | Chinese small iris high frequency regenerating system method is established based on callus induction |
| CN108496800B (en) * | 2018-04-10 | 2021-07-16 | 黑龙江省科学院自然与生态研究所 | Method for establishing iris high-frequency regeneration system based on callus induction |
| CN111887152A (en) * | 2019-11-19 | 2020-11-06 | 东北林业大学 | Method for establishing plant regeneration system of swallow flower bud growing way |
| CN111887152B (en) * | 2019-11-19 | 2022-06-07 | 东北林业大学 | Method for establishing plant regeneration system of swallow flower bud growing way |
| CN111567401A (en) * | 2020-04-20 | 2020-08-25 | 上海植物园 | Tissue culture rapid propagation method of butterfly flower' Mengdian |
| CN114403000A (en) * | 2021-12-31 | 2022-04-29 | 湖北懿昌能兴生态农业科技有限公司 | Louisiana iris tissue culture propagation method with high plant regeneration rate |
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Open date: 20080917 |