CN106386508A - Seedling method of rhodiola rosea and planting method of rhodiola rosea - Google Patents
Seedling method of rhodiola rosea and planting method of rhodiola rosea Download PDFInfo
- Publication number
- CN106386508A CN106386508A CN201611071316.5A CN201611071316A CN106386508A CN 106386508 A CN106386508 A CN 106386508A CN 201611071316 A CN201611071316 A CN 201611071316A CN 106386508 A CN106386508 A CN 106386508A
- Authority
- CN
- China
- Prior art keywords
- radix rhodiolae
- seed
- seedling
- culture
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a seedling method of rhodiola rosea and a planting method of the rhodiola rosea, and belongs to the field of plant production. The seedling method comprises the following steps: using a sodium hypochlorite solution with the mass concentration of 10% to 20% for peel removal and washing, inoculating seeds after peel removal into an MS culture medium containing vermiculite, indoleacetic acid with the mass concentration of 0.1 to 0.5 mg/L, gibberellin with the mass concentration of 0.5 to 1 mg/L and rare earth with the mass concentration of 0.01 to 0.05 mg/L, culturing at the temperature of 10 to 20 DEG C, irradiating for 10 to 12 h to carry out primary culture under the illumination intensity of 15500 to 18000 lx every day, inoculating obtained aseptic seedlings into a subculture culture medium, and then grafting obtained rootless seedlings into a rooting culture medium to obtain rhodiola rosea seedlings; after training the rhodiola rosea seedlings, cultivating the training seedlings into a green house, irradiating for 10 to 12 h under the illumination intensity of 15500 to 18000 lx every day, and matching with field management to obtain matured rhodiola rosea, wherein the emergence rate and seedling survival rate of rhodiola rosea are high.
Description
Technical field
A kind of a kind of the present invention relates to plant production field, and the kind particularly to method for culturing seedlings of Radix Rhodiolae and Radix Rhodiolae
Plant method.
Background technology
Radix Rhodiolae (Rhodiola rosea L.), another name：Rhodiola rosea L., sweep sieve agate boolean etc., be a kind of perennial grass
This plant.Root is sturdy, cone, and meat is isabelline, the most fibrous root of root cervical region tool, and rhizome is short, sturdy, cylindrical, is covered by majority
The lepidiod leaf of tiles arrangement, great majority are grown in the place about 3500-5000 rice, and Radix Rhodiolae is mainly entered with root and rhizome
Medicine, Herb also can be used as medicine.
Radix Rhodiolae is bitter：Sweet, bitter, flat, there is the kidney invigorating, qi-regulating blood-nourishing, promoting blood circulation and hemostasis, clearing away lung-heat to relieve cough, antipyretic, and under leukorrhagia stopping
Effect.
Because Radix Rhodiolae has splendid medical value, market constantly increases to the demand of Radix Rhodiolae, and
Only meet the demand in market by plucking wild Radix Rhodiolae, not only harvesting amount does not reach demand, and excessive harvesting also causes at Radix Rhodiolae
In endangered edge, belong to one-level Tibetan medicine in imminent danger material.Furthermore, Radix Rhodiolae is grown in High aititude region, the condition of its growth
Require harsh, artificial breeding, cultivation difficulty are larger, and the yield that further result in Radix Rhodiolae cannot meet the demand in market.
Content of the invention
It is an object of the invention to provide a kind of method for culturing seedlings of Radix Rhodiolae, after the seed of Radix Rhodiolae is removed the peel by it, artificially
Help the seed step that completes broken skin, shorten the time of seed germination；Add Vermiculitum to ensure to plant in Initial culture base
Son can carry out good breathing, advantageously emerging in seed in Initial culture base；Through successive transfer culture and taking root
After culture, the Radix Rhodiolae seedling that obtains, its incubation time from seed to seedling is short, and can effectively be lifted emergence rate and
Survival rate.
Another object of the present invention is to providing a kind of implantation methods of Radix Rhodiolae, by the seedling of Radix Rhodiolae through domestication
Afterwards it is easier to adapt to planting environment, improve the survival rate of Radix Rhodiolae seedling, the plantation yield of lifting Radix Rhodiolae, meet market
Demand, slows down the demand pressure of wild Radix Rhodiolae, avoids the extinction of Radix Rhodiolae to a certain extent.
The present invention solves its technical problem and employs the following technical solutions to realize.
A kind of method for culturing seedlings of Radix Rhodiolae, its be by the seed of Radix Rhodiolae be soaked in mass concentration be 10%-20% time
Sodium chlorate solution is removed the peel, and is rinsed with sterilized water after peeling, obtains removing the peel seed；Peeling seed is inoculated into just generation
In culture medium, carry out initial culture, obtain aseptic seedling；Using the cotyledon of aseptic seedling and hypocotyls as explant, and it is inoculated in and continues
In culture base, carry out successive transfer culture, obtain no root；No root will turn and plant in root media, and carry out root culture, obtain
To Radix Rhodiolae seedling；The temperature of initial culture is 10-20 DEG C, and intensity of illumination is 15500-18000lx, and light application time is 10-
12h, Initial culture base be added with Vermiculitum and mass concentration be the heteroauxing of 0.1-0.5mg/L, 0.5-1mg/L red mould
The MS culture medium of the rare earth of element and 0.01-0.05mg/L.
A kind of implantation methods of Radix Rhodiolae, it is to cultivate Radix Rhodiolae seedling according to the method for culturing seedlings of above-mentioned Radix Rhodiolae to tame and docile
Change, obtain taming Seedling；Seed will be tamed plant in booth, and control the light application time in booth to be 10-12h, intensity of illumination is
15500-18000lx, coordinates field management, to the Radix Rhodiolae obtaining maturation.
The beneficial effect of the implantation methods of the method for culturing seedlings of Radix Rhodiolae provided in an embodiment of the present invention and Radix Rhodiolae is：Pass through
Peeling to Radix Rhodiolae seed is processed, and accelerates the speed of Radix Rhodiolae seed germination, and by adding Vermiculitum in Initial culture base,
The breathing of seed can be conducive to, further speed up sprouting of seed；Rare earth can promote to sprout and the absorption to nutrition for the seed,
Improve the emergence rate of aseptic seedling further；In the presence of a series of tissue cultures of successive transfer culture and root culture, lifting is red
The emergence rate of Herba hylotelephii erythrosticti seed and survival rate, and strengthen the adaptability of Radix Rhodiolae seedling；By Radix Rhodiolae seedling through domestication
Plant again afterwards, can further lift the survival rate of Radix Rhodiolae seedling, increase the yield of Radix Rhodiolae, meet the demand in market,
Reduce the harvesting amount of wild Radix Rhodiolae, it is to avoid the danger of extinction in Radix Rhodiolae.
Specific embodiment
Purpose, technical scheme and advantage for making the embodiment of the present invention are clearer, below will be in the embodiment of the present invention
Technical scheme be clearly and completely described.In embodiment, unreceipted actual conditions person, builds according to normal condition or manufacturer
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, being can be by the commercially available conventional product bought and obtain
Product.
The implantation methods of the method for culturing seedlings to the Radix Rhodiolae of the embodiment of the present invention and Radix Rhodiolae are specifically described below.
The embodiment of the present invention provides a kind of method for culturing seedlings of Radix Rhodiolae, and it is the seed with liquor natrii hypochloritises to Radix Rhodiolae
Removed the peel, be rinsed with sterilized water after peeling, obtain removing the peel seed；Above-mentioned peeling seed through initial culture, continue
After culture and root culture, you can obtain Radix Rhodiolae seedling.
The embodiment of the present invention also provides a kind of implantation methods of Radix Rhodiolae, and it is through domestication by above-mentioned Radix Rhodiolae seedling
Afterwards, obtain taming Seedling；Seed will be tamed plant in booth, and control the light application time in booth to be 10-12h, intensity of illumination is
15500-18000lx, coordinates field management, to the Radix Rhodiolae obtaining maturation.
Specifically, the implantation methods of the method for culturing seedlings of Radix Rhodiolae and Radix Rhodiolae, are carried out according to following steps：
S1 peeling step：
The seed of Radix Rhodiolae is put in the container that can seal, such as：The plastic bottle of the PVC material with bottle cap, then
Pour the liquor natrii hypochloritises that mass concentration is 10%-20% into in the container filled seed, said vesse is sealed, uses this matter
Amount concentration is that the liquor natrii hypochloritises of 10%-20% soak seed 5-10min.Liquor natrii hypochloritises can be that kind of skin is soft de-
Fall, in addition to can be to seed peeling, can also sterilize it is ensured that can become in tissue culture procedures afterwards to seed disinfection
The carrying out of work(is it is ensured that the higher emergence rate of seed.
Preferably, during being soaked, seed can have been filled and liquor natrii hypochloritises' container shakes to above-mentioned
Swing, under the dual function of earthquake and liquor natrii hypochloritises, the aeration level of the crust of seed can increased.
Further, before pouring liquor natrii hypochloritises in said vesse, several stones can also be put in a reservoir
Son, after being filled with liquor natrii hypochloritises in container and start concussion, the stone of the inside vibrates together as well as vibration force, stone
In the collision of son and seed, part kind skin can be made to loosen faster, the work that the kind skin after being easy to completely removes.
Specifically, after immersion completes, remove the liquor natrii hypochloritises in container, and be placed in net by soaking the seed completing
In sieve, using aseptic water washing seed, while seed is pressed against on mesh screen being rubbed, so that the crust of seed is quickly taken off
Fall, and after the crust of seed comes off, continue to use aseptic water washing 20-30min, obtain removing the peel seed.With sterilized water to secondary
The seed that sodium chlorate solution soaked carries out the flushing of long period, can be effectively prevented from sodium hypochlorite and bring damage to seed
Wound, reduces bud ratio.
It is highly preferred that the seed that complete of peeling can also be laid in absorbent paper, and send into ultraviolet-sterilization case and carry out purple
Outer induction, the ultraviolet wavelength of above-mentioned ultraviolet induction is, for example, 250-254nm, and the time control of ultraviolet induction is 2-3h.Pass through
Above-mentioned ultraviolet induction, not only can give the peeling degerming sterilization of seed further, can also further improve the emergence rate of seed.
S2 initial culture:
The seed of peeling is inoculated in Initial culture base, carries out initial culture, obtain aseptic seedling.
Above-mentioned Initial culture base is to add the Vermiculitum and mass concentration indole second for 0.1-0.5mg/L in MS culture medium
Sour (IAA), the gibberellins (GA of 0.5-1mg/L3), the rare earth of 0.01-0.05mg/L prepare, the addition of above-mentioned Vermiculitum
Add 10-20g in above-mentioned MS culture medium for every 1L, and the particle diameter of this Vermiculitum is 6-8mm；Above-mentioned MS culture medium is commercially available
MS culture medium containing sucrose and agar.Heteroauxing can stimulate the division of cambial cell, stimulates the cell elongation of branch, suppression
The cell growth of root, adjusts the morphogenesis of calluss, makes an addition to and peeling seed can be promoted in MS culture medium quickly to go out
Bud, and lift the bud ratio of peeling seed, but the assurance to the amount of the heteroauxing adding is critically important, if the Yin adding
The amount of indolylbutyric acid excessively can suppress sprouting of seed on the contrary；Gibberellins can promote the elongation growth of stem, leaf, induces α-amylase
Formation, accelerate cell division, mature cell longitudinally elongation, internodal cell elongation, break seed dormancy, promote seed to sprout
Deng, in MS culture medium add gibberellins can further promote remove the peel seed rudiment；Rare earth is except promoting seed
Rudiment, improve seed germination rate, promote growth of seedling outside additionally it is possible to promote seed to the nutrient substance in culture medium
Absorb, thus further strengthening the bud ratio of seed；Make an addition to the Vermiculitum in culture medium, culture medium can be inoculated in
Seed provides enough oxygen can carry out enough breathings it is ensured that seed is inoculated in culture medium, if seed cannot be carried out
Normal Repiration, also results in the reduction of germination rate.When being used in mixed way of heteroauxing, gibberellins and rare earth, every kind of thing
The power of influence of confrontation seed germination rate is all improved, and so that the germination rate of peeling seed is further lifted.
Cultivation temperature during initial culture is 10-20 DEG C, and intensity of illumination is 15500-18000lx, and light application time is 10-
12h, and ambient humidity when cultivating is 45%-55%.Because the original producton location of Radix Rhodiolae, in high altitude localitiess, is being carried out just
In order to improve the bud ratio of seed during culture, the conditional regulatory of culture is become to meet the weather conditions of high altitude localitiess,
I.e. temperature difference during initial culture is larger, and illumination is stronger, and light application time is longer, and ambient humidity is low, the germination rate of lifting seed.
S3 successive transfer culture:
After initial culture, the cotyledon of the aseptic seedling obtaining and hypocotyls are inoculated in subculture medium as explant,
Carry out successive transfer culture, obtain no root.
It is that the cotyledon of the aseptic seedling of initial culture is cut blade tip, leaves petiole, and by petiole be cut into 0.3cm ×
The fritter of 0.3cm；The hypocotyls of aseptic seedling are cut into the segment of 0.3cm；Above-mentioned cotyledon piece and hypocotyls section are inoculated in and continue
On culture base, carry out successive transfer culture.Train after the cotyledon of aseptic seedling out from seed development and hypocotyls cutting again
Support, can effectively increase the yield of Radix Rhodiolae seedling, make a seed just can cultivate the seedling of many high-qualitys, greatly
The yield of lifting seedling.
The culture medium of successive transfer culture can be for example to add the 6- benzyl that mass concentration is 0.1-0.5mg/L in MS culture medium
Aminoadenine (6-BA), the gibberellins (GA of 1-1.5mg/L3) and the heteroauxing (IAA) of 0.5-1mg/L after obtain, its
In, above-mentioned MS culture medium is the commercially available MS culture medium containing sucrose and agar.6- benzyl aminoadenine can promote cell division,
Cell is promoted to increase weight gaining, the elongation growth promoting stem and leaf, suppression antibody Monoclonal ability that is aging, improving plant etc., in explant
Culture in, add 6-BA and can effectively lift differentiation and the resistance of explant, both can improve the emergence rate of explant；
IAA can stimulate the division of cambial cell, stimulate the cell elongation of branch, the cell growth of suppression root, adjust calluss
Morphogenesis, makes an addition to and can promote in MS culture medium quickly to form no root, lifts emergence rate；GA3Accelerate cell division, promote
Enter the elongation growth of stem, leaf, accelerate the growth of no root.In the presence of above-mentioned three Plant Hormones, being capable of synergism
Emerging in explant, further lifts the emergence rate of explant.
The cultivation temperature of successive transfer culture is preferably 5-15 DEG C, and intensity of illumination is 15500-18000lx, and light application time is 10-
12h, and culture ambient humidity be 45%-55%, its temperature, illumination condition and ambient humidity are close to the High aititude of Radix Rhodiolae
The environmental condition in original producton location, ensure that the emergence rate of explant, and lifts the resistance of the no root that explant is cultivated, and makes
The growing way of no root is higher, and survival rate is higher.
S4 root culture：
The no root that successive transfer culture is obtained turns plants in root media, carries out root culture, until obtaining Radix Rhodiolae
Seedling.
Successive transfer culture is to add the naphthalene acetic acid (NAA) that mass concentration is 1-1.5mg/L and 0.5-1mg/L in MS culture medium
Kinetins (KT) after obtain, above-mentioned MS culture medium is similarly the commercially available MS culture medium containing sucrose and agar.NAA is one
Plant the plant growth regulator promoting plant root growth, there is the effect promoting cell division and expanding, no root can be made
Quickly grow adventitious root.KT not only can promote cell division, with the differentiation of induced bud and growth and can increase pore
Aperture, thus lifting Phytoextraction carbon dioxide, increases the photosynthesis of plant, there to be more Nutrients uptakes no root
Growth and root of hair.
Temperature during root culture is 5-15 DEG C, and before no root sends out roots, carries out shading treatment, be conducive to unrooted
The taking root of Seedling, during root culture, ambient humidity is 45%-55%；Meanwhile, relatively low temperature and humidity condition is former close to Radix Rhodiolae
The high altitude environment in the place of production, not only contributes to taking root of no root, can also lift the resistance of Seedling, increases the survival of seedling
Rate.
The plantation of S5 Radix Rhodiolae：
The Radix Rhodiolae seedling cultivated according to the method described above, after domestication, obtains taming Seedling, then will tame seed plant
In booth, by a series of field management, obtain the Radix Rhodiolae of maturation.
Above-mentioned domestication refers to, by Radix Rhodiolae seedling cultivation in illumination box, intensity of illumination is set to 15500-
18000lx, and light application time be 10-12h, seedling in the whole nurturing period in illumination box, the humidity of illumination box
With 1h for interval, alternately press 70% and 50% setting.Replace cultivation by seedling is carried out with high humidity environment and low-humidity environment
Resistance training, enable Radix Rhodiolae more to adapt to the of a relatively high growing environment of humidity of low altitude area, carry further
Rise the survival ability after Radix Rhodiolae seedling enters greenhouse gardening.And above-mentioned illumination condition is close to the High aititude in Radix Rhodiolae original producton location
Illumination condition, it is ensured that the vigorous growth gesture of Radix Rhodiolae seedling, the survival rate of lifting transplanting seedling, also promotes the seedling energy of Radix Rhodiolae
Enough resistance training preferably adapting to different humidity.Have passed through the Radix Rhodiolae after the training of the resistance under different humidity transplanting in big
After canopy, its ratio of stock is higher, thus lifting the yield of Radix Rhodiolae.
When Radix Rhodiolae is planted in booth, intensity of illumination is preferably arranged to 15500-18000lx, and light application time is 10-
12h, it is possible to the work such as use cooperatively farm manure, cut weeds, enables the growth that Radix Rhodiolae is more vigorous, its yield can obtain
To lifting.
The implantation methods of the method for culturing seedlings to the Radix Rhodiolae of the present invention and Radix Rhodiolae are made further with reference to embodiments
Describe in detail.
Embodiment 1
Radix Rhodiolae seed is loaded in PVC plastic bottle, and puts into a small amount of stone in above-mentioned PVC plastic bottle, be then injected into
Mass concentration is 10% liquor natrii hypochloritises, covers tightly bottle cap, after soaking and shaking 10min, liquor natrii hypochloritises is removed, by bottle
In seed, pour in mesh screen, with aseptic water washing, rinse seed is pressed against on mesh screen and rub, until planting
Skin all removes, and continues to use aseptic water washing 20min.
The seed blotting the peeling of water is put in ultraviolet-sterilization case, processes 3h under the wavelength of 250nm.
By peeling seed be inoculated in containing 0.1mg/L heteroauxing, the gibberellins of 0.5mg/L, the rare earth of 0.05mg/L and
10g, size are in the Initial culture base of Vermiculitum of 6mm, temperature be 20 DEG C, intensity of illumination be that 15500lx, light application time are
10h and humidity are to be cultivated, until obtaining aseptic seedling under conditions of 45%.
The cotyledon of aseptic seedling is cut into the fritter of 0.3cm × 0.3cm, hypocotyls are cut into the segment of 0.3cm and inoculate respectively
In the subculture medium of 6- benzyl aminoadenine, 1mg/L gibberellins and 0.5mg/L heteroauxing containing 0.1mg/L, in temperature
Spend for 15 DEG C, intensity of illumination be 15500lx, light application time be the ambient humidity of 12h and culture be to cultivate under the conditions of 45%, directly
To obtaining no root.
In the root media of the kinetins no root transferred in the naphthalene acetic acid containing 1mg/L and 0.5mg/L, in temperature
For 15 DEG C, ambient humidity is 45%, is cultivated, until obtaining Radix Rhodiolae seedling under conditions of shading.
Radix Rhodiolae seedling is cultivated in illumination box, intensity of illumination is 15500lx, light application time is 12h, and light
According to incubator humidity with 1h for interval, alternately according to 70% and 50% setting, until obtain tame Seedling.
Domestication seed is planted it is ensured that the intensity of illumination in booth reaches 15500lx in booth, light application time reaches 10h,
And the Radix Rhodiolae of maturation is planted out in the work such as compounding application farm manure and weeding.
Embodiment 2
Radix Rhodiolae seed is loaded in PVC plastic bottle, and puts into a small amount of stone in above-mentioned PVC plastic bottle, be then injected into
Mass concentration is 20% liquor natrii hypochloritises, covers tightly bottle cap, after soaking and shaking 5min, liquor natrii hypochloritises is removed, by bottle
In seed, pour in mesh screen, with aseptic water washing, rinse seed is pressed against on mesh screen and rub, until planting
Skin all removes, and continues to use aseptic water washing 30min.
The seed blotting the peeling of water is put in ultraviolet-sterilization case, processes 2h under the wavelength of 254nm.
By peeling seed be inoculated in containing 0.5mg/L heteroauxing, the gibberellins of 1mg/L, the rare earth of 0.01mg/L and
20g, size are in the Initial culture base of Vermiculitum of 8mm, temperature be 10 DEG C, intensity of illumination be that 18000lx, light application time are
12h and humidity are to be cultivated, until obtaining aseptic seedling under conditions of 55%.
The cotyledon of aseptic seedling is cut into the fritter of 0.3cm × 0.3cm, hypocotyls are cut into the segment of 0.3cm and inoculate respectively
In the subculture medium of 6- benzyl aminoadenine, 1.5mg/L gibberellins and 1mg/L heteroauxing containing 0.5mg/L, in temperature
Spend for 5 DEG C, intensity of illumination be 18000lx, light application time be the ambient humidity of 10h and culture be to cultivate under the conditions of 55%, directly
To obtaining no root.
In the root media of the kinetins no root transferred in the naphthalene acetic acid containing 1.5mg/L and 1mg/L, in temperature
For 5 DEG C, ambient humidity is 55%, is cultivated, until obtaining Radix Rhodiolae seedling under conditions of shading.
Radix Rhodiolae seedling is cultivated in illumination box, intensity of illumination is 18000lx, light application time is 10h, and light
According to incubator humidity with 1h for interval, alternately according to 70% and 50% setting, until obtain tame Seedling.
Domestication seed is planted it is ensured that the intensity of illumination in booth reaches 18000lx in booth, light application time reaches 12h,
And the Radix Rhodiolae of maturation is planted out in the work such as compounding application farm manure and weeding.
Embodiment 3
Radix Rhodiolae seed is loaded in PVC plastic bottle, and puts into a small amount of stone in above-mentioned PVC plastic bottle, be then injected into
Mass concentration is 15% liquor natrii hypochloritises, covers tightly bottle cap, after soaking and shaking 7min, liquor natrii hypochloritises is removed, by bottle
In seed, pour in mesh screen, with aseptic water washing, rinse seed is pressed against on mesh screen and rub, until planting
Skin all removes, and continues to use aseptic water washing 25min.
The seed blotting the peeling of water is put in ultraviolet-sterilization case, processes 2.5h under the wavelength of 252nm.
By peeling seed be inoculated in containing 0.3mg/L heteroauxing, the gibberellins of 0.8mg/L, the rare earth of 0.03mg/L and
15g, size are in the Initial culture base of Vermiculitum of 7mm, temperature be 15 DEG C, intensity of illumination be that 16500lx, light application time are
11h and humidity are to be cultivated, until obtaining aseptic seedling under conditions of 50%.
The cotyledon of aseptic seedling is cut into the fritter of 0.3cm × 0.3cm, hypocotyls are cut into the segment of 0.3cm and inoculate respectively
In the subculture medium of 6- benzyl aminoadenine, 1.2mg/L gibberellins and 0.8mg/L heteroauxing containing 0.3mg/L,
Temperature is 10 DEG C, intensity of illumination is 17000lx, light application time is 11h and the ambient humidity of culture is culture under the conditions of 50%,
Until obtaining no root.
In the root media of the kinetins no root transferred in the naphthalene acetic acid containing 1.2mg/L and 0.7mg/L, in temperature
Spend for 10 DEG C, ambient humidity is 50%, is cultivated, until obtaining Radix Rhodiolae seedling under conditions of shading.
Radix Rhodiolae seedling is cultivated in illumination box, intensity of illumination is 17000lx, light application time is 11h, and light
According to incubator humidity with 1h for interval, alternately according to 70% and 50% setting, until obtain tame Seedling.
Domestication seed is planted it is ensured that the intensity of illumination in booth reaches 17000lx in booth, light application time reaches 11h,
And the Radix Rhodiolae of maturation is planted out in the work such as compounding application farm manure and weeding.
Embodiment 4
Radix Rhodiolae seed is loaded in PVC plastic bottle, and puts into a small amount of stone in above-mentioned PVC plastic bottle, be then injected into
Mass concentration is 16% liquor natrii hypochloritises, covers tightly bottle cap, after soaking and shaking 9min, liquor natrii hypochloritises is removed, by bottle
In seed, pour in mesh screen, with aseptic water washing, rinse seed is pressed against on mesh screen and rub, until planting
Skin all removes, and continues to use aseptic water washing 24min.
The seed blotting the peeling of water is put in ultraviolet-sterilization case, processes 2.2h under the wavelength of 251nm.
By peeling seed be inoculated in containing 0.2mg/L heteroauxing, the gibberellins of 0.8mg/L, the rare earth of 0.02mg/L and
12g, size are in the Initial culture base of Vermiculitum of 6mm, temperature be 18 DEG C, intensity of illumination be that 16500lx, light application time are
12h and humidity are to be cultivated, until obtaining aseptic seedling under conditions of 52%.
The cotyledon of aseptic seedling is cut into the fritter of 0.3cm × 0.3cm, hypocotyls are cut into the segment of 0.3cm and inoculate respectively
In the subculture medium of 6- benzyl aminoadenine, 1.3mg/L gibberellins and 0.8mg/L heteroauxing containing 0.2mg/L,
Temperature is 12 DEG C, intensity of illumination is 17500lx, light application time is 11.5h and the ambient humidity of culture is training under the conditions of 53%
Support, until obtaining no root.
In the root media of the kinetins no root transferred in the naphthalene acetic acid containing 1.4mg/L and 0.7mg/L, in temperature
Spend for 12 DEG C, ambient humidity is 52%, is cultivated, until obtaining Radix Rhodiolae seedling under conditions of shading.
Radix Rhodiolae seedling is cultivated in illumination box, intensity of illumination is 17500lx, light application time is 12h, and light
According to incubator humidity with 1h for interval, alternately according to 70% and 50% setting, until obtain tame Seedling.
Domestication seed is planted it is ensured that the intensity of illumination in booth reaches 16500lx in booth, light application time reaches 12h,
And the Radix Rhodiolae of maturation is planted out in the work such as compounding application farm manure and weeding.
Comparing embodiment 1, embodiment 2, the initial culture bud ratio of embodiment 3, embodiment 4 and matched group 1, wherein compare
The processing mode of group 1 is similar with embodiment, only difference is that, the seed of matched group 1 does not all remove kind of a skin.Emergence rate
=(quantity of the quantity/seed of aseptic seedling) × 100%, above-mentioned 5 groups are processed all according to 100 seeds calculating.Comparing result is shown in
Table 1.
The emergence rate of table 1 seed
| Group number | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Matched group 1 |
| Emergence rate | 95% | 93% | 91% | 93% | 71% |
According to comparing result, the emergence rate of the 4 groups of embodiments processing through peeling is not apparently higher than carrying out at peeling
The matched group 1 of reason.Illustrate, when carrying out nursery, to remove kind of a skin, can significantly improve the efficiency of seed sprouting.
Comparing embodiment 1, embodiment 2, the initial culture bud ratio of embodiment 3, embodiment 4 and matched group 2, wherein compare
The processing mode of group 2 is similar with embodiment, only difference is that, is not added with Vermiculitum in Initial culture base.Emergence rate=(no
The quantity of the quantity/seed of vaccine) × 100%, above-mentioned 5 groups are processed all according to 100 seeds calculating.Comparing result is shown in Table 2.
The emergence rate of table 2 seed
| Group number | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Matched group 2 |
| Emergence rate | 95% | 93% | 91% | 93% | 78% |
According to comparing result, the emergence rate that with the addition of Vermiculitum seed in Initial culture base is higher than to be not added with Vermiculitum
Matched group 2.Illustrate to the addition of the Repiration that Vermiculitum can be conducive to seed in Initial culture base, improve emergence rate.
Comparing embodiment 1, embodiment 2, the seedling cultivation survival rate of embodiment 3, embodiment 4 and matched group 3, wherein compare
The processing mode of group 3 is similar with embodiment, only difference is that, seedling cultivation was not being carried out before booth difference
The training of ambient humidity.Survival rate=(transplanting the transplanting quantity of the Seedling quantity/total of survival) × 100%, above-mentioned 5 groups of process are all pressed
Calculate according to 100 seedling.Comparing result is shown in Table 3.
The survival rate of table 3 seedling
| Group number | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Matched group 3 |
| Survival rate | 95% | 93% | 91% | 93% | 64% |
According to comparing result, the seedling of the 4 groups of embodiments survival rate in booth is higher than the survival rate of matched group 3,
Illustrate to carry out the training of different humidity before by seedling replanting to booth, the survival rate of seedling replanting can be lifted.
In sum, the implantation methods of the method for culturing seedlings of the Radix Rhodiolae of the embodiment of the present invention and Radix Rhodiolae, by red scape
The peeling of its seed is processed, and accelerates the speed of Radix Rhodiolae seed germination, and by adding Vermiculitum, Neng Gouyou in Initial culture base
Beneficial to the breathing of seed, further speed up sprouting of seed；Rare earth can promote to sprout and the absorption to nutrition for the seed, further
Improve the emergence rate of aseptic seedling；And in the presence of a series of tissue cultures of successive transfer culture and root culture, lift Radix Rhodiolae
The emergence rate of seed and survival rate, and strengthen the adaptability of Radix Rhodiolae seedling；Radix Rhodiolae seedling through after domestication again
Plantation, can further lift the survival rate of Radix Rhodiolae seedling, increase the yield of Radix Rhodiolae, meet the demand in market, reduce
The harvesting amount of wild Radix Rhodiolae, it is to avoid the danger of extinction in Radix Rhodiolae.
Embodiments described above is a part of embodiment of the present invention, rather than whole embodiments.The reality of the present invention
The detailed description applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected enforcement of the present invention
Example.Based on the embodiment in the present invention, those of ordinary skill in the art are obtained under the premise of not making creative work
Every other embodiment, broadly falls into the scope of protection of the invention.
Claims (10)
1. a kind of method for culturing seedlings of Radix Rhodiolae is it is characterised in that the seed of Radix Rhodiolae is soaked in mass concentration is 10%-20%
Liquor natrii hypochloritises removed the peel, be rinsed with sterilized water after peeling, obtain remove the peel seed；Described peeling seed is connect
Plant in Initial culture base, carry out initial culture, obtain aseptic seedling；Using the cotyledon of described aseptic seedling and hypocotyls as explant
Body, and be inoculated in subculture medium, carry out successive transfer culture, obtain no root；Described no root is turned and plants in root media
In, carry out root culture, obtain Radix Rhodiolae seedling；The temperature of described initial culture is 10-20 DEG C, and intensity of illumination is 15500-
18000lx, light application time is 10-12h, and described Initial culture base is to be added with Vermiculitum and mass concentration for 0.1-0.5mg/L
The rare earth of heteroauxing, the gibberellins of 0.5-1mg/L and 0.01-0.05mg/L MS culture medium.
2. Radix Rhodiolae according to claim 1 method for culturing seedlings it is characterised in that with described sterilized water be rinsed when
Between be 20-30min.
3. the method for culturing seedlings of Radix Rhodiolae according to claim 1 is it is characterised in that also include described peeling seed is being connect
Plant the step carrying out ultraviolet induction to before described Initial culture base, described ultraviolet induction is that described peeling seed is put into wavelength
For processing 2-3h under the ultraviolet of 250-254nm.
4. the method for culturing seedlings of Radix Rhodiolae according to claim 1 is it is characterised in that the temperature of described successive transfer culture is 5-15
DEG C, intensity of illumination is 15500-18000lx, and light application time is 10-12h.
5. the method for culturing seedlings of Radix Rhodiolae according to claim 1 is it is characterised in that the temperature of described root culture is 5-15
DEG C, and carry out shading treatment to turning the described no root planted in described root media.
6. the method for culturing seedlings of Radix Rhodiolae according to claim 1 is it is characterised in that described initial culture, described subculture are trained
Support and the ambient humidity of described root culture is 45%-55%.
7. the method for culturing seedlings of Radix Rhodiolae according to claim 1 is it is characterised in that gone with described liquor natrii hypochloritises
Skin is carried out in the following manner：Soak described seed 5-10min with described liquor natrii hypochloritises.
8. the method for culturing seedlings of Radix Rhodiolae according to claim 1 is it is characterised in that described subculture medium is to add to have matter
Amount concentration is the heteroauxing of the 6- benzyl aminoadenine, the gibberellins of 1mg-1.5mg/L and 0.5-1mg/L of 0.1-0.5mg/L
MS culture medium；Described root media is that to be added with mass concentration be swashing of the naphthalene acetic acid of 1mg-1.5mg/L and 0.5-1mg/L
The MS culture medium of therbligs.
9. a kind of implantation methods of Radix Rhodiolae are it is characterised in that nursery side by Radix Rhodiolae as described in any one of claim 1-8
The Radix Rhodiolae seedling domestication that method is cultivated, obtains taming Seedling；Described domestication seed is planted in booth, controls in described booth
Light application time is 10-12h, and intensity of illumination is 15500-18000lx, coordinates field management, to the Radix Rhodiolae obtaining maturation.
10. the implantation methods of Radix Rhodiolae according to claim 9 are it is characterised in that described domestication is by described Radix Rhodiolae
In illumination box, the intensity of illumination of described illumination box is 15500-18000lx to seedling cultivation, and light application time is 10-
12h, the humidity in described illumination box, with one hour for interval, alternately presses 70% and 50% setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611071316.5A CN106386508B (en) | 2016-11-29 | 2016-11-29 | The method for culturing seedlings of rhodiola root and the implantation methods of rhodiola root |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611071316.5A CN106386508B (en) | 2016-11-29 | 2016-11-29 | The method for culturing seedlings of rhodiola root and the implantation methods of rhodiola root |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106386508A true CN106386508A (en) | 2017-02-15 |
| CN106386508B CN106386508B (en) | 2018-05-04 |
Family
ID=58082179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611071316.5A Active CN106386508B (en) | 2016-11-29 | 2016-11-29 | The method for culturing seedlings of rhodiola root and the implantation methods of rhodiola root |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106386508B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108432764A (en) * | 2018-04-03 | 2018-08-24 | 洛阳超拓园林绿化工程有限公司 | A kind of root-growing agent improving palisades hole plant setting survival rate |
| CN112616674A (en) * | 2021-01-08 | 2021-04-09 | 南京农业大学 | Induced proliferation culture medium for tissue culture and rapid propagation of rhodiola rosea and tissue culture and rapid propagation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273028A (en) * | 2013-07-03 | 2015-01-14 | 中国科学院上海生命科学研究院 | Method for rapid in-vitro propagation of Crassulaceae plant |
-
2016
- 2016-11-29 CN CN201611071316.5A patent/CN106386508B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273028A (en) * | 2013-07-03 | 2015-01-14 | 中国科学院上海生命科学研究院 | Method for rapid in-vitro propagation of Crassulaceae plant |
Non-Patent Citations (3)
| Title |
|---|
| 王水琦: "《植物组织培养》", 31 March 2007, 中国轻工业出版社 * |
| 肖玉兰: "《植物无糖组培快繁工厂化生产技术》", 30 June 2003, 云南科技出版社 * |
| 胡颂平等: "《植物细胞组织培养技术》", 31 August 2014, 中国农业大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108432764A (en) * | 2018-04-03 | 2018-08-24 | 洛阳超拓园林绿化工程有限公司 | A kind of root-growing agent improving palisades hole plant setting survival rate |
| CN112616674A (en) * | 2021-01-08 | 2021-04-09 | 南京农业大学 | Induced proliferation culture medium for tissue culture and rapid propagation of rhodiola rosea and tissue culture and rapid propagation method |
| CN112616674B (en) * | 2021-01-08 | 2022-04-29 | 南京农业大学 | Induced proliferation culture medium for tissue culture and rapid propagation of rhodiola rosea and tissue culture and rapid propagation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106386508B (en) | 2018-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102119660B (en) | Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse | |
| CN103548652B (en) | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings | |
| CN104521715B (en) | Sealing water planting cutting propagation method for gardenias with flower buds | |
| CN106577281B (en) | The high planting percent breeding method of polygala arillata stem section tissue culture | |
| CN107347608A (en) | Virus-free seed potato and seedling water planting integration high power mating system | |
| CN106538196A (en) | A kind of breeding method of root of subprostrate sophora | |
| CN103125386B (en) | Industrial horseradish planting method | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN107155844A (en) | A kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication | |
| CN106069511A (en) | A kind of nursery stock inoculation method of Fructus Citri grandis | |
| CN104186351A (en) | Tissue culture method of strawberries | |
| CN106258956B (en) | A kind of dendrobium candidum pedigree breeding and field transplantation method | |
| CN105075858B (en) | A kind of liquid rapid propagation method of bletilla seed | |
| CN108293878A (en) | A kind of tissue culture method of snakegourd tender leaf | |
| CN107667860A (en) | A kind of tissue culture propagation of Alpinia japonica | |
| CN101627729B (en) | High-quality corm of colorful calla and method for cultivating same | |
| CN106359087A (en) | Tissue culture quick-breeding seedling raising method for radix asparagi | |
| CN106942063B (en) | A kind of paulownia tissue culture and rapid propagation method of combination outside sprout-cultivating-bottle technology | |
| CN104304000B (en) | A kind of Clones of Cunninghamia Lanceolata tissue cultured seedling root induction method and root media | |
| CN108739370A (en) | A method of it is carried out using ripe lotus embryo fast numerous | |
| CN104756866A (en) | Cuttage rapid propagation method of test-tube plantlet of toona sinensis | |
| CN103168690B (en) | Breeding method of Qi dioscorea opposita virus-free miniature seed beans | |
| CN105145363B (en) | It is a kind of to significantly improve the method that China fir tissue culture produces emergence rate | |
| CN103907497A (en) | Rapid cutting propagation method of test-tube plum plantlets | |
| CN106386508B (en) | The method for culturing seedlings of rhodiola root and the implantation methods of rhodiola root |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190311 Address after: 721000 Second Floor of Kiwifruit Industrial Park Testing Center, Meixian County, Baoji City, Shaanxi Province Patentee after: BAOJI SONGLIANG AGRICULTURAL TECHNOLOGY CO.,LTD. Address before: 266034 Room 1001-1002, 171 Shandong Road, North District, Qingdao City, Shandong Province Patentee before: QINGDAO SONGLIANG GENE TECHNOLOGY CO.,LTD. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221212 Address after: 266000 Room 902, 9/F, Building 7, Binhai 1, No. 201, Ruichang Road, Shibei District, Qingdao, Shandong Patentee after: Qingdao Songliang Zhiyun Technology Co.,Ltd. Address before: 721000 Second Floor of Kiwifruit Industrial Park Testing Center, Meixian County, Baoji City, Shaanxi Province Patentee before: BAOJI SONGLIANG AGRICULTURAL TECHNOLOGY CO.,LTD. |