CN104186062A - Method for germinating paris polyphylla seeds - Google Patents
Method for germinating paris polyphylla seeds Download PDFInfo
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- CN104186062A CN104186062A CN201410395352.1A CN201410395352A CN104186062A CN 104186062 A CN104186062 A CN 104186062A CN 201410395352 A CN201410395352 A CN 201410395352A CN 104186062 A CN104186062 A CN 104186062A
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Abstract
The invention discloses a method for germinating paris polyphylla seeds, belonging to the technical field of traditional Chinese medicine planting. The method for germinating paris polyphylla seeds comprises the following steps: (1) washing and peeling off paris polyphylla seeds, namely, putting ripe paris polyphylla seeds subjected to after-ripening into water, scrubbing to remove aril, and air-drying for later use; (2) freezing at low temperature, namely, freezing the air-dried paris polyphylla seeds for 2-3 months at 0-5 DEG C; and (3) culturing on a culture dish at constant temperature and humidity, namely, putting a piece of filtering paper into the culture dish at the middle third of January of the next year, putting the paris polyphylla seeds on the filtering paper, covering the seeds with the filtering paper, and culturing to the last third of April to the first third of May at constant temperature of 15-30 DEG C and constant humidity of 60-80 percent. The method is simple, convenient and feasible, the cost is low, the germination stage of the paris polyphylla seeds is shortened to be 4 to 5 months from two years, the germination rate is increased to be 80-90 percent from 30-50 percent, and technical guarantee is provided for propagation and on-scale planting of paris polyphylla.
Description
Technical field
The invention belongs to traditional Chinese medicine planting technology field, particularly the method for a kind of Yunnan Paris polyphylla seed germination.
Background technology
Yunnan Paris polyphylla [ParispolyphyllaSmithvar.yunnanensis (Franch.) Hand.-Mazz.], includes in one of the version Pharmacopoeia of the People's Republic of China in 2010, and tool is clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion effect; Swollen for furunculosis carbuncle, abscess of throat, snake bite and insect sting, falls and flutters the pain of injury, convulsion.Yunnan Paris polyphylla not only can directly be used as medicine, and is the main batching of the famous Chinese patent drugs such as " Yunnan Baiyao ", " palace blood is peaceful ", " heat poison is clear ", " jidesheng sheyao tablets ".For a long time, people rely on wild resource to Yunnan Paris polyphylla always.Due to excavating of long-term excessive predation formula, wild Paris polyphylla has suffered destructive destruction, and existing oneself is on the verge of exhaustion, and artificial planting becomes the inevitable choice that solves Paris polyphylla scarcity of resources.But the artificial planting of Yunnan Paris polyphylla is mainly to adopt directly plantation or the rhizome segment plantation of wild rhizome, this certainly will take a large amount of bulk drugs, cause with kind with the aggravation of contradictions of bulk drug, in the long run, the industrialization artificial cultivation of Yunnan Paris polyphylla should be from the breeding of seed.Yunnan Paris polyphylla seed has obvious afterripening, and Yunnan Paris polyphylla seed only has the incomplete little embryo of an oval differentiation, and embryo could be sprouted after needing dormancy to complete after-ripening, and Development and germination and the temperature of embryo are closely related.In natural situation, through the seedling of could being unearthed two winters, and emergence rate is lower.
Application number is 98118955.5, the Chinese patent that name is called " Yunnan Paris polyphylla seed sprouting and quick propagating technology thereof " discloses a kind of Yunnan Paris polyphylla seed sprouting and propagation method thereof, is specially: get Yunnan Paris polyphylla seed, water rinses, 70% alcohol immersion 2-5 minute, uses 0.2%HgC1
2soak 10-30 minute, in the gibberellin of 20-80mg/kg weight concentration, under 22 DEG C of dark conditions, soak 48 hours, be placed in sterile petri dish, under 4 DEG C or 22 DEG C of dark, alternately place each 1 week, 3-5 time repeatedly, then illumination every day 10 hours, light intensity 1000lux, taking 1/2MS as minimal medium, additional methyl α-naphthyl acetate (NAA) 0.5mg/L and 6-benzyl aminopurine (6BA) 0.1mg/L breed.The method can effectively be broken the Yunnan Paris polyphylla seed dormancy phase, reproduction speed is fast, the cycle is short, the advantage of industrial fast breeding, can effectively improve output and the quality of Yunnan Paris polyphylla.
Application number is 201310153037.3, and the Chinese patent that name is called " a kind of method that improves Yunnan Paris polyphylla seed germination rate " discloses a kind of method that improves Yunnan Paris polyphylla seed germination rate.The method is rubbed with the hands and is removed exosper again after the ripe Yunnan Paris polyphylla fruit band pericarp gathering is dried in the shade, and goes the seed of exosper to carry out high temperature immersion stranding: the seed 48 hours that removes exosper with the emerge in worm water stranding of 40 DEG C.Injure again processing: the seed that high temperature is soaked is placed on coarse metallic plate crumples its seed coat, carry out again subsequently gibberellin and soak rear alternating temperature Sand culture.Yunnan Paris polyphylla seed germination rate is brought up to more than 96%.
Application number is 200810058414.4, and the Chinese patent that name is called " one is broken the seed dormant method of Yunnan Paris polyphylla " discloses one and broken the seed dormant method of Yunnan Paris polyphylla.The concrete steps of the method are: by the Yunnan Paris polyphylla seed of the redness maturation gathering, wash away exocarp in the annual 9-10 month, naturally dry in the shade 15 days under room temperature, pack subsequently ventilative valve bag into and preserve.Take out the seed in valve bag November, and the water soaking under room temperature 15 hours takes out and at room temperature naturally dries 15 hours subsequently, puts into afterwards water again and soaks 15 hours.By through the seed of above-mentioned processing directly sowing in soil, sprout, thickness of earth covering 2cm, waters and is advisable with ponding not, seed is sprouted for 60 days in soil afterwards, its germination rate, more than 90%, can be widely used in the cultivation of Yunnan Paris polyphylla.
Application number is 200910084213.6, and the Chinese patent that name is called " germination accelerating method of a kind of Yunnan Paris polyphylla seed " discloses the germination accelerating method of a kind of Yunnan Paris polyphylla seed.The method is to mix with 2-4:1 ratio with Yunnan Paris polyphylla seed with the wet sand of water content 60-80%, places 25-35 days for 8-15 DEG C; Take out the GA of seed 50-200mg/L
3solution seed soaking 1.5-2.5 hour; Mix with 2-4:1 ratio with seed with the wet sand of water content 60-80% again, place 18-25 DEG C, keep wet husky moisture content 55-70 days; The GA that is 2.5-30mg/L by concentration again after taking-up seed
3solution seed soaking 1.5-3 hour; Mix with 2-4:1 ratio with seed with the wet sand of water content 60-80%, keep wet husky moisture content.The method of this invention can make Yunnan Paris polyphylla seed sprout in advance, and emergence rate is high.
Above-mentioned patented technology, has been used HgC1
2, ethanol, gibberellin, GA
3, the compound such as NAA, 6BA, not only increased production cost, and may exist chemical substance residual, be unfavorable for Yunnan Paris polyphylla standardized cultivation.
By literature search, have no the open report identical with the present invention.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of method that new Yunnan Paris polyphylla seed germination is provided, the method can be broken the Yunnan Paris polyphylla seed dormancy phase, shortens the budding period of Yunnan Paris polyphylla seed, accelerate the sprouting of Yunnan Paris polyphylla seed, improve the germination rate of Yunnan Paris polyphylla seed.
Object of the present invention is practised by following technical proposals:
A method for Yunnan Paris polyphylla seed germination, comprises the steps:
(1) Paris polyphylla seed in Yunnan cleans decortication: wash arillus by rubbing with the hands by putting into water through the ripe Yunnan Paris polyphylla seed of after-ripening, dry moisture for subsequent use.
(2) deepfreeze: dry the Yunnan Paris polyphylla seed of moisture in 0～5 DEG C of refrigeration 2～3 months.
(3) culture dish constant temperature and humidity is cultivated: in mid-January next year, in culture dish, place filter paper, on filter paper, place Yunnan Paris polyphylla seed, then cover filter paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
Step (1) Yunnan Paris polyphylla seed cleans decortication and is preferably: in 9～October, red the ripe Yunnan Paris polyphylla of gathering seed, put shady and cool place's after-ripening 10～20 days; Put into water again and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking, dries moisture for subsequent use.
Step (2) deepfreeze is preferably: the Yunnan Paris polyphylla seed that dries moisture will be put into 4 DEG C of refrigerator deepfreezes 2～3 months.
The placement density of the Yunnan Paris polyphylla seed described in step (3) is preferably 30～50/dm
2.
Step (3) culture dish constant temperature and humidity is cultivated and is preferably: in mid-January next year, place 10～15 metafiltration paper in culture dish, place Yunnan Paris polyphylla seed on filter paper, density is 30～50/dm
2, then cover 5～10 metafiltration paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
Preferred, the method for a kind of Yunnan Paris polyphylla seed germination, comprises the steps:
(1) Paris polyphylla seed in Yunnan cleans decortication: in 9～October, red the ripe Yunnan Paris polyphylla of gathering seed, put shady and cool place's after-ripening 15 days; Put into water again and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking, dries moisture for subsequent use.
(2) deepfreeze: the Yunnan Paris polyphylla seed that dries moisture will be put into 4 DEG C of refrigerator deepfreezes 2～3 months.
(3) culture dish constant temperature and humidity is cultivated: in mid-January next year, place 10～15 metafiltration paper in culture dish, place Yunnan Paris polyphylla seed on filter paper, density is 30～50/dm
2, then cover 5～10 metafiltration paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
Advantage of the present invention and beneficial effect are: the present invention is simple and easy to do, with low cost, and the budding period of Yunnan Paris polyphylla was shortened to 4～5 months from 2 years, and germination rate brings up to 80～90% from 30～50%.Breeding and large-scale planting that the present invention is Yunnan Paris polyphylla provide technical guarantee.
Brief description of the drawings
Fig. 1 is the sprouting situation map that Yunnan Paris polyphylla seed culture ware constant temperature and humidity is cultivated.
Fig. 2 is the sprouting situation map that Yunnan Paris polyphylla seed culture ware constant temperature and humidity is cultivated.
Fig. 3 is Paris polyphylla seed germination situation map in earth culture Yunnan under equal conditions.
Fig. 4 is Paris polyphylla seed germination situation map in Sha Pei Yunnan under equal conditions.
Embodiment
The method of Yunnan provided by the invention Paris polyphylla seed germination, comprises the steps:
(1) Paris polyphylla seed in Yunnan cleans decortication: wash arillus by rubbing with the hands by putting into water through the ripe Yunnan Paris polyphylla seed of after-ripening, dry moisture for subsequent use.
(2) deepfreeze: dry the Yunnan Paris polyphylla seed of moisture in 0～5 DEG C of refrigeration 2～3 months.
(3) culture dish constant temperature and humidity is cultivated: in mid-January next year, in culture dish, place filter paper, on filter paper, place Yunnan Paris polyphylla seed, then cover filter paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
Below in conjunction with specific embodiment, the present invention is done to further detailed description.Should be understood that the following examples are only not used in and limit the scope of the invention for the present invention is described.
Embodiment 1
The present embodiment is in the Yunnan Province Agriculture Academy of Science Medicine Plant Institute laboratory implementation of Kunming, Yunnan city.
(1), in 9～October, red the ripe Yunnan Paris polyphylla of gathering seed, put shady and cool place's after-ripening 15 days.
(2) the Yunnan Paris polyphylla seed of after-ripening is put into water and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking, dries moisture for subsequent use.
(3) the Yunnan Paris polyphylla seed that dries moisture is put into 4 DEG C of refrigerator deepfreezes 2～3 months.
(4) in mid-January next year, in diameter 10cm culture dish, place 10 metafiltration paper, on filter paper, place 30～50 Yunnan Paris polyphylla seeds, then cover 5～10 metafiltration paper;
Adopt respectively two kinds of methods of earth culture and Sha Pei in contrast simultaneously, in soil or sand, keep humidity 60～80%, cladding thickness 2cm Yunnan Paris polyphylla planting seed.
(5) keep culture dish and Yunnan Paris polyphylla seed temperature at 15～30 DEG C, humidity is 60～80%, and constant temperature and humidity is cultured to the first tenday period of a month in late April to May; Earth culture and Sha Pei be in identical environmental condition, waters not occur that ponding is advisable.
While being cultured to mid-April, more than 86% all there is rudiment or bud point (Fig. 1, Fig. 2) in the Yunnan Paris polyphylla seed of constant temperature and humidity culture dish method of the present invention; The Yunnan Paris polyphylla seed of earth culture method only has 67% germination rate (Fig. 3); The Yunnan Paris polyphylla seed of Sha Peifa is not sprouted (Fig. 4).
After the Paris polyphylla seed germination of Yunnan, be transplanted in time seedbed management.
The present embodiment shows that the inventive method is simple and easy to do, with low cost, and the seed germination time is short, and Yunnan Paris polyphylla seed germination rate, apparently higher than earth culture method, reaches more than 86%.Cultivation by the method for Yunnan Paris polyphylla, is subject to the welcome at Paris polyphylla plantation family, Yunnan deeply.
Claims (7)
1. a method for Yunnan Paris polyphylla seed germination, is characterized in that comprising the steps:
(1) Paris polyphylla seed in Yunnan cleans decortication: wash arillus by rubbing with the hands by putting into water through the ripe Yunnan Paris polyphylla seed of after-ripening, dry moisture for subsequent use;
(2) deepfreeze: dry the Yunnan Paris polyphylla seed of moisture in 0～5 DEG C of refrigeration 2～3 months;
(3) culture dish constant temperature and humidity is cultivated: in mid-January next year, in culture dish, place filter paper, on filter paper, place Yunnan Paris polyphylla seed, then cover filter paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
2. the method for Yunnan according to claim 1 Paris polyphylla seed germination, is characterized in that: step (1) Yunnan Paris polyphylla seed cleans decortication and is: in 9～October, red the ripe Yunnan Paris polyphylla of gathering seed, put shady and cool place's after-ripening 15 days; Put into water again and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking, dries moisture for subsequent use.
3. the method for Yunnan according to claim 1 Paris polyphylla seed germination, is characterized in that: step (2) deepfreeze is: the Yunnan Paris polyphylla seed that dries moisture will be put into 4 DEG C of refrigerator deepfreezes 2～3 months.
4. the method for Yunnan according to claim 1 Paris polyphylla seed germination, is characterized in that: the placement density of the Yunnan Paris polyphylla seed described in step (3) is 30～50/dm
2.
5. the method for Yunnan according to claim 1 Paris polyphylla seed germination, it is characterized in that: step (3) culture dish constant temperature and humidity is cultivated and is: in mid-January next year, in culture dish, place 10～15 metafiltration paper, place Yunnan Paris polyphylla seed on filter paper, density is 30～50/dm
2, then cover 5～10 metafiltration paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
6. the method for Yunnan according to claim 1 Paris polyphylla seed germination, is characterized in that: comprise the steps:
(1) Paris polyphylla seed in Yunnan cleans decortication: in 9～October, red the ripe Yunnan Paris polyphylla of gathering seed, put shady and cool place's after-ripening 15 days; Put into water again and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking, dries moisture for subsequent use;
(2) deepfreeze: the Yunnan Paris polyphylla seed that dries moisture will be put into 4 DEG C of refrigerator deepfreezes 2～3 months;
(3) culture dish constant temperature and humidity is cultivated: in mid-January next year, place 10～15 metafiltration paper in culture dish, place Yunnan Paris polyphylla seed on filter paper, density is 30～50/dm
2, then cover 5～10 metafiltration paper, under 15～30 DEG C of temperature, humidity 60～80% conditions, constant temperature and humidity is cultured to the first tenday period of a month in late April to May.
7. the method for Yunnan according to claim 1 Paris polyphylla seed germination, is characterized in that: after the Paris polyphylla seed germination of Yunnan, be transplanted in time seedbed management.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982217A (en) * | 2015-08-11 | 2015-10-21 | 玉龙县凤翔旺生态养殖有限责任公司 | Paris polyphylla seed propagating method |
| CN104996103A (en) * | 2015-06-26 | 2015-10-28 | 中国中医科学院中药研究所 | Pseudo-ginseng seed storage method |
| CN105103855A (en) * | 2015-08-13 | 2015-12-02 | 临沧双滢生物科技有限公司 | Method of promoting paris polyphylla mother seeds to germinate in advance |
| CN105248278A (en) * | 2015-10-16 | 2016-01-20 | 金寨县绿野中药材专业合作社 | Method for raising seed seedlings of rhizoma paridis chinensis |
| CN105248093A (en) * | 2015-10-19 | 2016-01-20 | 中国科学院昆明植物研究所 | Method increasing Paris polyphylla var yunnanensis maturing rate |
| CN105325143A (en) * | 2015-10-14 | 2016-02-17 | 中国科学院昆明植物研究所 | Paris polyphylla var yunnanensis and paris polyphylla var chinensis seedling three-phase breeding technology |
| CN105612997A (en) * | 2015-12-29 | 2016-06-01 | 云南彝道农业发展有限公司 | Method for raising emergence rate of seeds of typhonium giganteum engl. |
| CN105746129A (en) * | 2016-03-03 | 2016-07-13 | 湖南利诺生物药业有限公司 | Rapid propagation method for polyphylla seeds |
| CN106031342A (en) * | 2015-03-18 | 2016-10-19 | 云南宝田农业科技有限公司 | Method for geminating Rhizoma Paridis Yunnanensis |
| CN107258294A (en) * | 2017-07-21 | 2017-10-20 | 广西桂平市蒙圩镇柱强种养专业合作社 | A kind of breeding method of Yunnan Paris polyphylla seed |
| CN107360753A (en) * | 2017-07-20 | 2017-11-21 | 四川省中医药科学院 | A kind of magnificent Paris polyphylla seed fast breeding method |
| CN107548727A (en) * | 2017-10-19 | 2018-01-09 | 中国农业大学 | A kind of storage practice of Yunnan Paris polyphylla seed |
| CN108307965A (en) * | 2018-03-14 | 2018-07-24 | 上海市药材有限公司 | A kind of vernalization of paris polyphylla seed and method for culturing seedlings |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1486591A (en) * | 2003-08-02 | 2004-04-07 | 腾冲县生物技术开发有限责任公司 | Artificial Rhizoma paridis seedling growing process |
| CN1602656A (en) * | 2004-11-01 | 2005-04-06 | 成都中康光大药业有限公司 | Wild Paris chinensis Franch artificial domestication grow seedling and cultivation method |
| CN101147441A (en) * | 2007-10-18 | 2008-03-26 | 云南省农业科学院高山经济植物研究所 | Seedlings growing method for promoting emergence rate of paris yunnanensis franch seeds |
| JP2009232755A (en) * | 2008-03-27 | 2009-10-15 | National Agriculture & Food Research Organization | Treatment method for improving germination |
-
2014
- 2014-08-12 CN CN201410395352.1A patent/CN104186062B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1486591A (en) * | 2003-08-02 | 2004-04-07 | 腾冲县生物技术开发有限责任公司 | Artificial Rhizoma paridis seedling growing process |
| CN1602656A (en) * | 2004-11-01 | 2005-04-06 | 成都中康光大药业有限公司 | Wild Paris chinensis Franch artificial domestication grow seedling and cultivation method |
| CN101147441A (en) * | 2007-10-18 | 2008-03-26 | 云南省农业科学院高山经济植物研究所 | Seedlings growing method for promoting emergence rate of paris yunnanensis franch seeds |
| JP2009232755A (en) * | 2008-03-27 | 2009-10-15 | National Agriculture & Food Research Organization | Treatment method for improving germination |
Non-Patent Citations (2)
| Title |
|---|
| 熊海浪 等: "滇重楼种子萌发及组织培养研究", 《广东农业科学》, no. 21, 31 October 2011 (2011-10-31) * |
| 陈翠 等: "云南重楼种苗繁育技术", 《中国现代中药》, vol. 12, no. 2, 28 February 2010 (2010-02-28) * |
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| CN106031342A (en) * | 2015-03-18 | 2016-10-19 | 云南宝田农业科技有限公司 | Method for geminating Rhizoma Paridis Yunnanensis |
| CN104996103A (en) * | 2015-06-26 | 2015-10-28 | 中国中医科学院中药研究所 | Pseudo-ginseng seed storage method |
| CN104982217A (en) * | 2015-08-11 | 2015-10-21 | 玉龙县凤翔旺生态养殖有限责任公司 | Paris polyphylla seed propagating method |
| CN105103855A (en) * | 2015-08-13 | 2015-12-02 | 临沧双滢生物科技有限公司 | Method of promoting paris polyphylla mother seeds to germinate in advance |
| CN105103855B (en) * | 2015-08-13 | 2017-08-08 | 临沧福大吉农林开发有限公司 | A kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time |
| CN105325143A (en) * | 2015-10-14 | 2016-02-17 | 中国科学院昆明植物研究所 | Paris polyphylla var yunnanensis and paris polyphylla var chinensis seedling three-phase breeding technology |
| CN105248278A (en) * | 2015-10-16 | 2016-01-20 | 金寨县绿野中药材专业合作社 | Method for raising seed seedlings of rhizoma paridis chinensis |
| CN105248093A (en) * | 2015-10-19 | 2016-01-20 | 中国科学院昆明植物研究所 | Method increasing Paris polyphylla var yunnanensis maturing rate |
| CN105612997A (en) * | 2015-12-29 | 2016-06-01 | 云南彝道农业发展有限公司 | Method for raising emergence rate of seeds of typhonium giganteum engl. |
| CN105746129A (en) * | 2016-03-03 | 2016-07-13 | 湖南利诺生物药业有限公司 | Rapid propagation method for polyphylla seeds |
| CN105746129B (en) * | 2016-03-03 | 2018-06-29 | 湖南利诺生物药业有限公司 | Paris polyphylla seed fast breeding method |
| CN107360753A (en) * | 2017-07-20 | 2017-11-21 | 四川省中医药科学院 | A kind of magnificent Paris polyphylla seed fast breeding method |
| CN107258294A (en) * | 2017-07-21 | 2017-10-20 | 广西桂平市蒙圩镇柱强种养专业合作社 | A kind of breeding method of Yunnan Paris polyphylla seed |
| CN107548727A (en) * | 2017-10-19 | 2018-01-09 | 中国农业大学 | A kind of storage practice of Yunnan Paris polyphylla seed |
| CN108307965A (en) * | 2018-03-14 | 2018-07-24 | 上海市药材有限公司 | A kind of vernalization of paris polyphylla seed and method for culturing seedlings |
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