A kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time
Technical field
The present invention relates to biological medicinal material Yunnan Paris polyphylla seed growing expanding species method, the technology directly applies to biological medicinal material and bred
Expanding species.
Background technology
At present, Yunnan Paris polyphylla seed growing technology, which relates generally to one kind, makes Yunnan Paris polyphylla seed come into leaves for four months long, eight months
Processing method, including its germination technology step is:The first step, the Yunnan Paris polyphylla seed capsules for selecting at the beginning of September maturation, the moon of making thinner
It is dry 30 days;Second step, at the beginning of 10 months will under the Yunnan Paris polyphylla seed cryogenic conditions that dried in the shade place 15~20 days carry out low-temperature treatment;The
Three steps, in mid-October by complete low-temperature treatment after Yunnan Paris polyphylla seed peel off capsule shell, by the Yunnan Paris polyphylla seed with red skin
It is sown in ready seed plate, carries out seed sprouting;4th step, the seed plate of the 3rd step is placed in freezer, low-temperature treatment 15
~20 days;5th step, mid-January by the later Yunnan Paris polyphylla seed broadcasting of the 4th step low-temperature treatment in the crop field of 2800 meters of height above sea level
In;6th step, carry out artificial weeder and make, begin with Yunnan Paris polyphylla seedling mid-April and come into leaves, there is 85% Yunnan weight to mid-June
Building seedling grows leaf, and the method makes the bud ratio and planting percent of Paris polyphylla seed reach more than 85%.(it is Patent point see this
Analysis is 201410502503.9)
In view of Yunnan Paris polyphylla germination needs low temperature to dry, the maturity of its original seed is most important, therefore, the present invention
Made an issue of in the maturity for focusing on Yunnan Paris polyphylla original seed, the Yunnan Paris polyphylla original seed received is selected using 9~October, makes 4, original seed with this invention
The moon comes into leaves;In addition, in raising technology, during processing Yunnan Paris polyphylla original seed, using " secondary dormancy " technology, promoting original seed to send out in advance
Bud.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of bud ratio and planting percent for making Yunnan Paris polyphylla original seed and reached
More than 80% method for promoting the germination ahead of time of Yunnan Paris polyphylla original seed.
The technical scheme that the present invention solves above-mentioned technical problem is as follows:A kind of side of promotion Yunnan Paris polyphylla original seed germination ahead of time
Method, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 42~48 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
12~18 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 42~48 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in treated nursery, according to every square metre
The density of 130~160 seeds is sowed;After planting, sandy soil layer and pine needle layer are laid in nursery after planting successively, to protect
The wetness of soil is demonstrate,proved, is watered once within every 8~12 days;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 80%~97% grows leaf.
The beneficial effects of the invention are as follows:Shorten the Yunnan Paris polyphylla original seed budding cycle.Can be by other Stratification techniques with the present invention
Growth cycle 360 days, is in advance 120 days, greatly shortens the original seed budding time, while bud ratio is improved, increased economy
Effect.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Further, the thickness of the sandy soil layer is 1.5~2cm.
Further, the thickness of the pine needle layer is 2.0~2.5cm.
Further, a kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 45 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
15 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 45 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in treated nursery, according to every square metre
The density of 150 seeds is sowed;After planting, 2cm sandy soil layer is covered, 2.25cm pine needle layer is covered again above, to ensure soil
The wetness of earth, waters once for every 10 days;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 97% grows leaf.
Further, a kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 42 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
12 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 42 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in treated nursery, according to every square metre
The density of 130 seeds is sowed;After planting, 1.75cm sandy soil layer is covered, 2.0cm pine needle layer is covered again above, to ensure
The wetness of soil, waters once for every 8 days;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 85% grows leaf.
Further, a kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 48 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
18 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 48 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in nursery, according to the density of 160 every square metre
Sow;After planting, 1.5cm sandy soil layer is covered, 2.5cm pine needle layer is covered again above, to ensure the wetness of soil, every 12
It waters once;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 80% grows leaf.
Further, the processing of the nursery will pass through following steps:
1) the whole moisture in the soil in crop field, per moisture in the soil by 1.5 meters wide, rises high 0.20 meter;
2) by step 1) in whole thin time of crop field put down;
3) to step 2) in crop field sprinkling soil sterilants, dry in the air to after 24 hours, obtain nursery, sow original seed.
Beneficial effect using above-mentioned further scheme is:
1st, original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed, makes original
Selection is planted to be optimal;
2nd, " secondary dormancy " method is used, Yunnan Paris polyphylla original seed obtains endoplasm structure evolution twice and dried twice, makes original seed kind
Son is able to fully revive, and makes its sprout growth in advance;
3rd, sow density using 150 at the aspect of sowing of Yunnan Paris polyphylla original seed, make its sprout after spacing in the rows it is neat, density is fitted
In, while effectively using soil, suitable shoot growth;
4th, after Paris polyphylla original seed in Yunnan is sowed, 2 centimetres of sandy soil of covering, and cover pine needle, make its keep suitable wetness and
Gas permeability, to ensure 97% bud ratio.
5th, after being sowed using Yunnan Paris polyphylla original seed every moisturizing in 10 days once, it is ensured that soil moisture is suitable, is conducive to seed to sprout
Bud, fries bud beneficial to seed and is unearthed.
Embodiment
The principle and feature to the present invention are described with reference to embodiments, and the given examples are served only to explain the present invention,
It is not intended to limit the scope of the present invention.
Embodiment one
A kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 45 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
15 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 45 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in treated nursery, according to every square metre
The density of 150 seeds is sowed;After planting, 2cm sandy soil layer is covered, 2.25cm pine needle layer is covered again above, to ensure soil
The wetness of earth, waters once for every 10 days;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 97% grows leaf.
The processing of the nursery will pass through following steps:
1) the whole moisture in the soil in crop field, per moisture in the soil by 1.5 meters wide, rises high 0.20 meter;
2) by step 1) in whole thin time of crop field put down;
3) to step 2) in crop field sprinkling soil sterilants, dry in the air to after 24 hours, obtain nursery, sow original seed.
Embodiment two
A kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 42 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
12 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 42 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in treated nursery, according to every square metre
The density of 130 seeds is sowed;After planting, 1.75cm sandy soil layer is covered, 2.0cm pine needle layer is covered again above, to ensure
The wetness of soil, waters once for every 8 days;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 85% grows leaf.
The processing of the nursery will pass through following steps:
1) the whole moisture in the soil in crop field, per moisture in the soil by 1.5 meters wide, rises high 0.20 meter;
2) by step 1) in whole thin time of crop field put down;
3) to step 2) in crop field sprinkling soil sterilants, dry in the air to after 24 hours, obtain nursery, sow original seed.
Embodiment three
A kind of method of promotion Yunnan Paris polyphylla original seed germination ahead of time, comprises the following steps:
(1) original seed is selected:Ripe Yunnan Paris polyphylla capsule during selecting for 9~October, making thinner to dry in the shade obtains primary original seed;
(2) dormancy:The primary original seed that step (1) is obtained is dried, and after primary original seed removes steam, is put into
In self-sealing plastic bag, preserved 48 days in a low temperature of 0~2 degree of refrigerator-freezer;
(3) once dissipate and dry in the air:The original seed that step (2) is obtained takes out from refrigerator-freezer and valve bag, scattered sun in shady place put to
18 days;
(4) secondary dormancy:The original seed that step (3) is obtained is put into valve bag, is inserted in refrigerator-freezer in 0~2 degree of low temperature again
Preserve 48 days;
(5) secondary dissipate is dried in the air:The original seed that step (4) is obtained peels off capsule shell, places shady place and dries removal epidermis water
Point;
(6) original seed is broadcasted sowing:The original seed that step (5) is obtained is sown in nursery, according to the density of 160 every square metre
Sow;After planting, 1.5cm sandy soil layer is covered, 2.5cm pine needle layer is covered again above, to ensure the wetness of soil, every 12
It waters once;
(7) weeding seedling-protection:The weeds grown are removed in cultivating process, Yunnan Paris polyphylla is begun with and grows leaflet June next year, to 7
The Yunnan Paris polyphylla seedling that the end of month has 80% grows leaf.
The processing of the nursery will pass through following steps:
1) the whole moisture in the soil in crop field, per moisture in the soil by 1.5 meters wide, rises high 0.20 meter;
2) by step 1) in whole thin time of crop field put down;
3) to step 2) in crop field sprinkling soil sterilants, dry in the air to after 24 hours, obtain nursery, sow original seed.
In summary, the specific experiment situation of three kinds of embodiments is as follows:
Example one, when Yunnan Paris polyphylla original seed Cord blood 42 days in refrigerator-freezer, original seed storage life is shorter, more normal storage life
(45 days) few 72 hours, and its endoplasm structure evolution not exclusively, thus influences its bud ratio;Example two, when Yunnan Paris polyphylla original seed is in ice
In cabinet during Cord blood 45 days, original seed storage life is in normal range (NR), and its endoplasm structure evolution completely, thus improves budding
Rate;Example three, when Yunnan Paris polyphylla original seed Cord blood 48 days in refrigerator-freezer, original seed storage life is longer, more normal storage life (45
My god) 72 hours more, its endoplasm structure evolution was in isloation state, thus equally influenceed its bud ratio;
Example one, when Yunnan Paris polyphylla original seed is dried 12 days, the evaporation of original seed contained humidity is insufficient, and water content is higher, and influence is former
Plant bud ratio;Example two, when Yunnan Paris polyphylla original seed is dried 15 days, the evaporation of original seed contained humidity is abundant, and water content is moderate, hereby it is ensured that
The bud ratio of original seed;Example three, when Yunnan Paris polyphylla original seed is dried 18 days, original seed contained humidity evaporation capacity is too high, makes its water content inclined
It is low, thus the influence normal bud ratio of original seed;
, example one about the same with " dormancy " situation during " secondary dormancy " is implemented, when Yunnan Paris polyphylla original seed is in refrigerator-freezer
During Cord blood 42 days, original seed storage life is shorter, and more normal storage life (45 days) is few 72 hours, and its endoplasm structure develops not again
Completely, thus its bud ratio is influenceed;Example 2, when Yunnan Paris polyphylla original seed Cord blood 45 days in refrigerator-freezer, original seed storage life is just
In normal scope, its endoplasm structure develops completely again, thus improves bud ratio;Example 3, when Yunnan Paris polyphylla original seed in refrigerator-freezer it is low
When temperature is preserved 48 days, original seed storage life is longer, more normal storage life (45 days) 72 hours more, and its endoplasm structure develops again to be in
Isloation state is crossed, thus equally influences its bud ratio;
Example one, when sowing 130, Yunnan Paris polyphylla original seed for every square metre, spacing in the rows is excessively dilute after budding, and be unfavorable for soil has
Effect is utilized;Example two, when sowing 150, Yunnan Paris polyphylla original seed for every square metre, spacing in the rows is neat after budding, and density is moderate, effective
While using soil, suitable shoot growth;Example three, when sowing Yunnan Paris polyphylla original seed more than 160 for every square metre, after budding
Spacing in the rows is overstocked, and influence young shoot light, ventilation, growth;
Example one, after Yunnan Paris polyphylla original seed is sowed, cover 1.5 centimetres of sandy soil, influence its moisturizing degree, make bud ratio by
Loss;Example two, after Yunnan Paris polyphylla original seed is sowed, covers 2 centimetres of sandy soil, it is ensured that soil moisture, good permeability, it is ensured that go out
Bud rate;Example three, after Yunnan Paris polyphylla original seed is sowed, covers 2.5 centimetres of sandy soil, have impact on the gas permeability of soil, increase soil
Water content, bud ratio is incurred loss;Cover uniform pine needle again above, preferably pine needle, it is good ventilative to be first that it has
Property, next to that harvesting is easy in locality, reduce cost;Other can also use rice straw mulching, but straw is after repeatedly watering, careless bar
Fouling, adds humidity, destroys gas permeability, the growth after influence original seed budding;
To ensure the wetness of soil, it is necessary to which every 10 days or so water once;Example one, Yunnan Paris polyphylla original seed sow after every
Once, soil moisture is too high, and seed easily rots for moisturizing in 8 days, is unfavorable for the fried bud of seed and is unearthed;Example two, Yunnan Paris polyphylla original seed is broadcast
After spreading every moisturizing in 10 days once, soil moisture is suitable, is conducive to seed germination, and frying bud beneficial to seed is unearthed;Example three, Yunnan weight
Building original seed sow after every moisturizing in 12 days once, soil moisture is too low, reduce the seed germination time, be also unfavorable for seed fry bud go out
Soil.
The thickness of the pine needle layer is 2.0~2.5cm, guarantor's wetness of soil can be effectively ensured, less than this standard
Do not reach guarantor's wetness of soil, the ventilating air permeability of original seed is have impact on again after this standard.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.