CN109005734A - A kind of method of Seed of Camellia oleifera sawdust vernalization - Google Patents
A kind of method of Seed of Camellia oleifera sawdust vernalization Download PDFInfo
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- CN109005734A CN109005734A CN201810787563.8A CN201810787563A CN109005734A CN 109005734 A CN109005734 A CN 109005734A CN 201810787563 A CN201810787563 A CN 201810787563A CN 109005734 A CN109005734 A CN 109005734A
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- sawdust
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- camellia oleifera
- methylglycine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a kind of method of Seed of Camellia oleifera sawdust vernalization, include the following steps: that (one) Seed of Camellia oleifera screens；(2) it is soaked seed respectively with water and germination liquid；(3) sawdust prepares；(4) vernalization.The germination liquid includes the component of following parts by weight: 0.2-0.5 parts of rape element sterol, 0.005-0.01 parts of gibberellin, 0.002-0.005 parts of thiophanate methyl, 8-10 parts, 95-100 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.The method of Seed of Camellia oleifera sawdust vernalization disclosed by the invention is easy to operate, Germination effect is good, the bud ratio and high survival rate of seed, the emergence rate of plant is high, uniformity and later period growth power are good, the date of seeding of oil tea can effectively be shifted to an earlier date, achieve the purpose that early sowing early emergence, increase yield, there are preferable economic benefit, social benefit and ecological benefits.
Description
Technical field
The present invention relates to presprouting of seeds technical field more particularly to a kind of storage sides of oil tea breeding fringe bar heat preserving antistaling
Method.
Background technique
Oil tea is the distinctive high-quality woody oil tree species of south China, tea oil color clear flavor incense, it is prolonged it is resistance to deposit, not by aspergillus flavus
Element infection, be not easy it is rancid, it is easily digested, long-term consumption have good medical care effect to human body, be current depth
By the favorite green of people, healthy edible vegetable oil.Tea oil in addition to edible or vaseline, hair oil, machine oil and
Pharmaceutically make the raw material of penicillin and streptomycin finish.
The seed of oil tea can because external environment be not suitable for or own growth is immature, cause the dormant period of seed long, send out
Bud rate is low, and sprouting, irregular and germination quality is low, emerge situations such as postponing, and is after planting not easy to germinate under natural conditions or germinate
Slowly or even seed goes mouldy and rots, and the planting percent of nursery is lower, has seriously affected the growth of later period oil tea, results in growth week
A series of problems, such as phase is long, and product quality is not high influences economic benefit.To oil tea Accelerating germination before sowing, oil tea can be effectively shortened
The germinating time of seed and germination percentage is improved, and shift to an earlier date the date of seeding of oil tea with this, reached early sowing early emergence, increase yield, improve
The purpose of business efficiency.But Seed of Camellia oleifera germination accelerating method in the prior art is not directed to the specific requirements vernalization of all kinds of seeds,
Effective strain can not be carried out to environment, type etc., cause the waste of resource and specifically used effect undesirable.
Therefore, it is necessary to seek more efficiently Seed of Camellia oleifera germination accelerating method, Seed of Camellia oleifera vernalization in the prior art is overcome to imitate
The undesirable technical problem of fruit ensures the bud ratio and survival rate of seed, emergence rate, uniformity and the later period growth power of plant.
Summary of the invention
The main purpose of the present invention is to provide a kind of method of Seed of Camellia oleifera sawdust vernalization, the germination accelerating method, operation letters
Just, Germination effect is good, the bud ratio and high survival rate of seed, and the emergence rate of plant is high, uniformity and later period growth power are good.Can have
Effect shift to an earlier date oil tea date of seeding, achieve the purpose that early sowing early emergence, increase yield, have preferable economic benefit, social benefit and
Ecological benefits.
To achieve the above objectives, the technical solution adopted by the present invention are as follows: a kind of method of Seed of Camellia oleifera sawdust vernalization, including
Following steps:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 1-2 hours, in removing
Floating impurity and blighted grain seed is then placed in warm water and impregnates 18-25 hours, finally soaked seed 1-2 hours with germination liquid again, rear to drip
Solid carbon dioxide point is stand-by；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 4-5 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then one
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on layer sawdust, another layer sawdust irrigates the height of 2000 times of liquid
Potassium manganate water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 12- in cool-bag
Vernalization in 20 days.
Preferably, the mesh number of No. 1 iron sieve is 10-30 mesh；The mesh number of No. 3 iron sieve is 100-200 mesh；Described 5
The mesh number of number iron sieve is 4-10 mesh.
Preferably, the temperature of the warm water is 25-35 DEG C；One layer of sawdust with a thickness of 10cm-15cm.
Preferably, the temperature of the cool-bag is controlled at 25~35 DEG C, and humid control is 80~90%.
Preferably, the moisturizing material is selected from infiltration one of sponge or wet cloth.
Preferably, the germination liquid includes the component of following parts by weight: 0.2-0.5 parts of rape element sterol, gibberellin
0.005-0.01 parts, 0.002-0.005 parts of thiophanate methyl, phosphate three (methylol) methylglycine jasmine hydrochlorate 8-10
Part, 95-100 parts of water.
Preferably, the preparation method of phosphate three (methylol) the methylglycine jasmine hydrochlorate, includes the following steps:
Three (methylol) methylglycines are dissolved in water by I forms solution, then potassium hydroxide is added into solution, until solution PH
=7, water is evaporated off in back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite being prepared by step I are dissolved in tetrahydrofuran and form solution by II, so
Diethyl (2- ethyl chloride) phosphate is added thereto afterwards, is stirred to react at 40-60 DEG C 6-8 hours, tetrahydro is evaporated off in back spin
Furans, and washed 3-5 times with ether, obtain phosphate three (methylol) methylglycine villaumite；
III phosphate three (methylol) the methylglycine villaumite formation solution soluble in water that will be prepared by step II,
Jasmonic is added in backward solution, is stirred to react at 40-60 DEG C 4-6 hours, the chlorination that water is evaporated off in back spin and reaction generates
Hydrogen obtains phosphate three (methylol) methylglycine jasmine hydrochlorate.
Preferably, (methylol) methylglycine three described in step I, water mass ratio be 1:(5-10).
Preferably, (methylol) methylglycine sylvite, tetrahydrofuran, diethyl (2- ethyl chloride) three described in step II
Phosphatic mass ratio is 1:(5-8): 1.37.
Preferably, (methylol) methylglycine of phosphate three described in step III villaumite, water, jasmonic mass ratio be
3.54:(10-12):1。
Preferably, the preparation method of the germination liquid includes the following steps: to mix each raw material, with 800-1000 revs/min
The mixing speed mechanical stirring of clock 20-30 minutes, obtains germination liquid.
Due to the above technical solutions, the present invention has the following advantages over the prior art:
(1) method of Seed of Camellia oleifera sawdust vernalization provided by the invention, easy to operate, Germination effect is good；
(2) method of Seed of Camellia oleifera sawdust vernalization provided by the invention, overcome in the prior art Seed of Camellia oleifera Germination effect pay no attention to
The technical issues of thinking, the bud ratio and survival rate of energy effective guarantee seed, emergence rate, uniformity and the later period growth power of plant,
So that the bud ratio and high survival rate of seed, the emergence rate of plant is high, uniformity and later period growth power are good.In addition, can effectively mention
The date of seeding of preceding oil tea, achievees the purpose that early sowing early emergence, increases yield, has preferable economic benefit, social benefit and ecology
Benefit；
(3) method of Seed of Camellia oleifera sawdust vernalization provided by the invention improves the water conservation energy of seed by the dipping of germination liquid
Power, antibacterial ability, and the elements such as N/P/K are introduced, further improve Germination effect；
(4) method of Seed of Camellia oleifera sawdust vernalization provided by the invention, each ingredient synergistic effect of germination liquid, so that its Germination effect
Significantly, rape element sterol, gibberellin are inside added, may advantageously facilitate rudiment and growth；Thiophanate methyl is added, is further mentioned
High sterilizing ability plays water conservation, vernalization, supplies nutrients by introducing phosphate three (methylol) methylglycine jasmine hydrochlorate
Multiple action.
Specific embodiment
It is described below for disclosing the present invention so that those skilled in the art can be realized the present invention.It is excellent in being described below
Embodiment is selected to be only used as illustrating, it may occur to persons skilled in the art that other obvious modifications.
Embodiment 1
A kind of method of Seed of Camellia oleifera sawdust vernalization, includes the following steps:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 1 hour, removes and floats
Impurity and blighted grain seed, be then placed in warm water and impregnate 18 hours, finally soaked seed 1 hour with germination liquid again, after drain away the water
For use；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 4 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then at one layer
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on sawdust, another layer sawdust irrigates the Gao Meng of 2000 times of liquid
Sour potassium water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 12 in cool-bag
Its vernalization.
The mesh number of No. 1 iron sieve is 10 mesh；The mesh number of No. 3 iron sieve is 100 mesh；The mesh number of No. 5 iron sieve is 4
Mesh.
The temperature of the warm water is 25 DEG C；One layer of sawdust with a thickness of 10cm.
The temperature of the cool-bag is controlled at 25 DEG C, and humid control is 80%.
The moisturizing material is infiltration sponge.
The germination liquid includes the component of following parts by weight: 0.2 part of rape element sterol, 0.005 part of gibberellin, methyl Tobe
0.002 part of saliva, 8 parts, 95 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of phosphate three (methylol) the methylglycine jasmine hydrochlorate, includes the following steps:
Three (methylol) methylglycine 10g are dissolved in water 50g by I forms solution, then potassium hydroxide is added into solution, until
Water is evaporated off in solution PH=7, back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite 10g being prepared by step I are dissolved in tetrahydrofuran 50g and being formed by II
Then diethyl (2- ethyl chloride) phosphate 13.7g is added thereto, is stirred to react at 40 DEG C 6 hours, back spin is evaporated off for solution
Tetrahydrofuran is removed, and is washed 3 times with ether, phosphate three (methylol) methylglycine villaumite is obtained；
Phosphate three (methylol) the methylglycine villaumite 17.7g being prepared by step II is dissolved in shape in water 50g by III
At solution, jasmonic 5g is added in backward solution, is stirred to react at 40 DEG C 4 hours, back spin is evaporated off water and reacts generation
Hydrogen chloride obtains phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of the germination liquid includes the following steps: to mix each raw material, with 800 revs/min of mixing speed
Mechanical stirring 20 minutes, obtain germination liquid.
Embodiment 2
A kind of method of Seed of Camellia oleifera sawdust vernalization, includes the following steps:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 1.2 hours, in removing
Floating impurity and blighted grain seed is then placed in warm water and impregnates 19 hours, is finally soaked seed 1.2 hours with germination liquid again, after drain
Moisture is stand-by；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 4.2 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then one
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on layer sawdust, another layer sawdust irrigates the height of 2000 times of liquid
Potassium manganate water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 13 in cool-bag
Its vernalization.
The mesh number of No. 1 iron sieve is 15 mesh；The mesh number of No. 3 iron sieve is 120 mesh；The mesh number of No. 5 iron sieve is 6
Mesh.
The temperature of the warm water is 28 DEG C；One layer of sawdust with a thickness of 12cm.
The temperature of the cool-bag is controlled at 28 DEG C, and humid control is 83%.
The moisturizing material is wet cloth.
The germination liquid includes the component of following parts by weight: 0.3 part of rape element sterol, 0.007 part of gibberellin, methyl Tobe
0.003 part of saliva, 8.5 parts, 97 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of phosphate three (methylol) the methylglycine jasmine hydrochlorate, includes the following steps:
Three (methylol) methylglycine 10g are dissolved in water 65g by I forms solution, then potassium hydroxide is added into solution, until
Water is evaporated off in solution PH=7, back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite 10g being prepared by step I are dissolved in tetrahydrofuran 62g and being formed by II
Then diethyl (2- ethyl chloride) phosphate 13.7g is added thereto, is stirred to react at 45 DEG C 6.5 hours for solution, rear to rotate
Tetrahydrofuran is removed, and is washed 4 times with ether, phosphate three (methylol) methylglycine villaumite is obtained；
Phosphate three (methylol) the methylglycine villaumite 17.7g being prepared by step II is dissolved in shape in water 53g by III
At solution, jasmonic 5g is added in backward solution, is stirred to react at 45 DEG C 4.5 hours, water is evaporated off in back spin and reaction generates
Hydrogen chloride, obtain phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of the germination liquid includes the following steps: to mix each raw material, with 850 revs/min of mixing speed
Mechanical stirring 23 minutes, obtain germination liquid.
Embodiment 3
A kind of method of Seed of Camellia oleifera sawdust vernalization, includes the following steps:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 1.5 hours, in removing
Floating impurity and blighted grain seed is then placed in warm water and impregnates 22 hours, is finally soaked seed 1.6 hours with germination liquid again, after drain
Moisture is stand-by；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 4.6 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then one
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on layer sawdust, another layer sawdust irrigates the height of 2000 times of liquid
Potassium manganate water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 16 in cool-bag
Its vernalization.
The mesh number of No. 1 iron sieve is 20 mesh；The mesh number of No. 3 iron sieve is 160 mesh；The mesh number of No. 5 iron sieve is 8
Mesh.
The temperature of the warm water is 30 DEG C；One layer of sawdust with a thickness of 13cm.
The temperature of the cool-bag is controlled at 30 DEG C, and humid control is 86%.
The moisturizing material is infiltration sponge.
The germination liquid includes the component of following parts by weight: 0.35 part of rape element sterol, 0.008 part of gibberellin, methyl support
0.004 part of cloth saliva, 9 parts, 98 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of phosphate three (methylol) the methylglycine jasmine hydrochlorate, includes the following steps:
Three (methylol) methylglycine 10g are dissolved in water 85g by I forms solution, then potassium hydroxide is added into solution, until
Water is evaporated off in solution PH=7, back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite 10g being prepared by step I are dissolved in tetrahydrofuran 70g and being formed by II
Then diethyl (2- ethyl chloride) phosphate 13.7g is added thereto, is stirred to react at 50 DEG C 7.2 hours for solution, rear to rotate
Tetrahydrofuran is removed, and is washed 5 times with ether, phosphate three (methylol) methylglycine villaumite is obtained；
Phosphate three (methylol) the methylglycine villaumite 17.7g being prepared by step II is dissolved in shape in water 57g by III
At solution, jasmonic 5g is added in backward solution, is stirred to react at 52 DEG C 5.2 hours, water is evaporated off in back spin and reaction generates
Hydrogen chloride, obtain phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of the germination liquid includes the following steps: to mix each raw material, with 900 revs/min of mixing speed
Mechanical stirring 20-30 minutes, obtain germination liquid.
Embodiment 4
A kind of method of Seed of Camellia oleifera sawdust vernalization, includes the following steps:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 1.8 hours, in removing
Floating impurity and blighted grain seed is then placed in warm water and impregnates 24 hours, is finally soaked seed 1.8 hours with germination liquid again, after drain
Moisture is stand-by；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 4.8 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then one
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on layer sawdust, another layer sawdust irrigates the height of 2000 times of liquid
Potassium manganate water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 19 in cool-bag
Its vernalization.
The mesh number of No. 1 iron sieve is 26 mesh；The mesh number of No. 3 iron sieve is 190 mesh；The mesh number of No. 5 iron sieve is 8
Mesh.
The temperature of the warm water is 33 DEG C；One layer of sawdust with a thickness of 14cm.
The temperature of the cool-bag is controlled at 33 DEG C, and humid control is 88%.
The moisturizing material is wet cloth.
The germination liquid includes the component of following parts by weight: 0.45 part of rape element sterol, 0.01 part of gibberellin, methyl Tobe
0.004 part of saliva, 9.5 parts, 99 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of phosphate three (methylol) the methylglycine jasmine hydrochlorate, includes the following steps:
Three (methylol) methylglycine 10g are dissolved in water 95g by I forms solution, then potassium hydroxide is added into solution, until
Water is evaporated off in solution PH=7, back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite 10g being prepared by step I are dissolved in tetrahydrofuran 75g and being formed by II
Then diethyl (2- ethyl chloride) phosphate 13.7g is added thereto, is stirred to react at 58 DEG C 7.5 hours for solution, rear to rotate
Tetrahydrofuran is removed, and is washed 4 times with ether, phosphate three (methylol) methylglycine villaumite is obtained；
Phosphate three (methylol) the methylglycine villaumite 17.7g being prepared by step II is dissolved in shape in water 59g by III
At solution, jasmonic 5g is added in backward solution, is stirred to react at 58 DEG C 5.8 hours, water is evaporated off in back spin and reaction generates
Hydrogen chloride, obtain phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of the germination liquid includes the following steps: to mix each raw material, with 950 revs/min of mixing speed
Mechanical stirring 29 minutes, obtain germination liquid.
Embodiment 5
A kind of method of Seed of Camellia oleifera sawdust vernalization, includes the following steps:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 2 hours, removes and floats
Impurity and blighted grain seed, be then placed in warm water and impregnate 25 hours, finally soaked seed 2 hours with germination liquid again, after drain away the water
For use；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 5 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then at one layer
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on sawdust, another layer sawdust irrigates the Gao Meng of 2000 times of liquid
Sour potassium water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 20 in cool-bag
Its vernalization.
The mesh number of No. 1 iron sieve is 30 mesh；The mesh number of No. 3 iron sieve is 200 mesh；The mesh number of No. 5 iron sieve is
10 mesh.
The temperature of the warm water is 35 DEG C；One layer of sawdust with a thickness of 15cm.
The temperature of the cool-bag is controlled at 35 DEG C, and humid control is 90%.
The moisturizing material is wet cloth.
The germination liquid includes the component of following parts by weight: 0.5 part of rape element sterol, 0.01 part of gibberellin, methyl Tobe
0.005 part of saliva, 10 parts, 100 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of phosphate three (methylol) the methylglycine jasmine hydrochlorate, includes the following steps:
Three (methylol) methylglycine 10g are dissolved in water 100g by I forms solution, then potassium hydroxide is added into solution, until
Water is evaporated off in solution PH=7, back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite 10g being prepared by step I are dissolved in tetrahydrofuran 80g and being formed by II
Then diethyl (2- ethyl chloride) phosphate 13.7g is added thereto, is stirred to react at 60 DEG C 8 hours, back spin is evaporated off for solution
Tetrahydrofuran is removed, and is washed 5 times with ether, phosphate three (methylol) methylglycine villaumite is obtained；
Phosphate three (methylol) the methylglycine villaumite 17.7g being prepared by step II is dissolved in shape in water 60g by III
At solution, jasmonic 5g is added in backward solution, is stirred to react at 60 DEG C 6 hours, back spin is evaporated off water and reacts generation
Hydrogen chloride obtains phosphate three (methylol) methylglycine jasmine hydrochlorate.
The preparation method of the germination liquid includes the following steps: to mix each raw material, with 1000 revs/min of stirring speed
Degree mechanical stirring 30 minutes, obtains germination liquid.
Comparative example 1
Germination accelerating method is similar to Example 1, the difference is that not impregnated with germination liquid.
Comparative example 2
Germination accelerating method is the same as sowing vernalization related in 107494167 A embodiment 1 of CN.
Presoaking and germinating is carried out to same a collection of Seed of Camellia oleifera to above-described embodiment 1-5 and comparative example 1-2 is utilized respectively, simultaneously
Blank group is set, clear water presoaking and germinating is as used, after 30 hours, 50 hours, 70 hours and final bud ratio is shown in Table 1.
Table 1
As can be seen from the above table, using the final bud ratio 99-100% of method vernalization Seed of Camellia oleifera disclosed by the embodiments of the present invention, 70
Bud ratio 80-95% after hour, and method vernalization in the prior art is used, final bud ratio 92%, bud ratio after 70 hours
63%, if, without being soaked seed using germination liquid, final bud ratio 90% sprouts after 70 hours using the germination accelerating method of the present embodiment
Rate 73%, it is seen that it is more preferable using germination accelerating method Germination effect disclosed in inventive embodiments, it is more efficient.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and what is described in the above embodiment and the description is only the present invention
Principle, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these variation and
Improvement is both fallen in the range of claimed invention.The present invention claims protection scope by appended claims and its
Equivalent defines.
Claims (10)
1. a kind of method of Seed of Camellia oleifera sawdust vernalization, which comprises the steps of:
(1) Seed of Camellia oleifera screens: by the artificial oil tea fresh fruit for shining and coming out, classification, bulky grain is weeded out with No. 1 iron, little particle
Or yellow also with the sieve screening of No. 3 iron, take the tea oil seed of intermediate full seed；
(2) it soaks seed: the appropriate Seed of Camellia oleifera by screening storage being put into clear water before sowing and is first impregnated 1-2 hours, in removing
Floating impurity and blighted grain seed is then placed in warm water and impregnates 18-25 hours, finally soaked seed 1-2 hours with germination liquid again, rear to drip
Solid carbon dioxide point is stand-by；
(3) sawdust prepares: retract No. 5 iron of sawdust from band saw factory and screen out impurity, substantially remove bigger bark or
Then sawdust dries sawdust 4-5 days, the fine degree according to weather, it is ensured that mass dryness fraction reaches 90% or more, then one
Sawdust is irrigated with the potassium permanganate water that sprayer sprays into 2000 times of liquid on layer sawdust, another layer sawdust irrigates the height of 2000 times of liquid
Potassium manganate water, so three times；
(4) vernalization: hand picks up then unclamping naturally for sawdust, and sawdust, which slightly scatters, to be advisable, after smooth, by ready Seed of Camellia oleifera
It is sprinkling upon on sawdust, can't see the sawdust below seed, almost has the thickness of 2 seeds, then put the sawdust of 10cm above,
The potassium permanganate water of 2000 times of liquid is sprayed, then covers moisturizing material above, then places it in and carries out 12- in cool-bag
Vernalization in 20 days.
2. the method for Seed of Camellia oleifera sawdust vernalization according to claim 1, which is characterized in that the mesh number of No. 1 iron sieve
For 10-30 mesh；The mesh number of No. 3 iron sieve is 100-200 mesh；The mesh number of No. 5 iron sieve is 4-10 mesh.
3. the method for Seed of Camellia oleifera sawdust vernalization according to claim 1, which is characterized in that the temperature of the warm water is
25-35℃；One layer of sawdust with a thickness of 10cm-15cm.
4. the method for Seed of Camellia oleifera sawdust vernalization according to claim 1, which is characterized in that the temperature of the cool-bag
Control is at 25~35 DEG C, and humid control is 80~90%.
5. the method for Seed of Camellia oleifera sawdust vernalization according to claim 1, which is characterized in that the moisturizing material, which is selected from, to be seeped
One of water sponge or wet cloth.
6. the method for Seed of Camellia oleifera sawdust vernalization according to claim 1, which is characterized in that the germination liquid includes as follows
The component of parts by weight: 0.2-0.5 parts of rape element sterol, 0.005-0.01 parts of gibberellin, 0.002-0.005 parts of thiophanate methyl,
8-10 parts, 95-100 parts of water of phosphate three (methylol) methylglycine jasmine hydrochlorate.
7. the method for Seed of Camellia oleifera sawdust vernalization according to claim 6, which is characterized in that three (the hydroxyl first of phosphate
Base) methylglycine jasmine hydrochlorate preparation method, include the following steps:
Three (methylol) methylglycines are dissolved in water by I forms solution, then potassium hydroxide is added into solution, until solution PH
=7, water is evaporated off in back spin, obtains three (methylol) methylglycine sylvite；
Three (methylol) methylglycine sylvite being prepared by step I are dissolved in tetrahydrofuran and form solution by II, so
Diethyl (2- ethyl chloride) phosphate is added thereto afterwards, is stirred to react at 40-60 DEG C 6-8 hours, tetrahydro is evaporated off in back spin
Furans, and washed 3-5 times with ether, obtain phosphate three (methylol) methylglycine villaumite；
III phosphate three (methylol) the methylglycine villaumite formation solution soluble in water that will be prepared by step II,
Jasmonic is added in backward solution, is stirred to react at 40-60 DEG C 4-6 hours, the chlorination that water is evaporated off in back spin and reaction generates
Hydrogen obtains phosphate three (methylol) methylglycine jasmine hydrochlorate.
8. the method for Seed of Camellia oleifera sawdust vernalization according to claim 7, which is characterized in that (hydroxyl first three described in step I
Base) methylglycine, water mass ratio be 1:(5-10).
9. the method for Seed of Camellia oleifera sawdust vernalization according to claim 7, which is characterized in that three (hydroxyl described in step II
Methyl) methylglycine sylvite, tetrahydrofuran, diethyl (2- ethyl chloride) phosphatic mass ratio be 1:(5-8): 1.37；Step
The villaumite of (methylol) methylglycine of phosphate three described in rapid III, water, jasmonic mass ratio be 3.54:(10-12): 1.
10. a kind of preparation method of germination liquid described in claim 6, which comprises the steps of: mix each raw material
It closes, with mixing speed mechanical stirring 20-30 minutes of 800-1000 revs/min, obtains germination liquid.
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| CN110024660A (en) * | 2019-04-17 | 2019-07-19 | 雷军 | A kind of method and vernalization soil making vernalization soil using sawdust as raw material |
| CN111328567A (en) * | 2020-02-26 | 2020-06-26 | 株洲市地杰现代农业有限责任公司 | Camellia oleifera seedling breeding method |
| CN113692904A (en) * | 2021-08-05 | 2021-11-26 | 贵州黔西南喀斯特区域发展研究院(黔西南州农业林业科学研究院) | Method for storing camellia oleifera seeds and cultivating bud seedling stocks by using sawdust |
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Application publication date: 20181218 |