CN106069137A - The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in a kind of booth - Google Patents

The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in a kind of booth Download PDF

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Publication number
CN106069137A
CN106069137A CN201610527790.8A CN201610527790A CN106069137A CN 106069137 A CN106069137 A CN 106069137A CN 201610527790 A CN201610527790 A CN 201610527790A CN 106069137 A CN106069137 A CN 106069137A
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China
Prior art keywords
booth
cultured seedling
tissue cultured
herba anoectochili
anoectochili roxburghii
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CN201610527790.8A
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Chinese (zh)
Inventor
陈阳春
张本厚
顾正国
张伟
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INSTITUTE OF DAFENG MARINE INDUSTRY NANJING UNIVERSITY OF TECHNOLOGY
Jiangsu Daocheng Biological Science & Technology Co Ltd
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INSTITUTE OF DAFENG MARINE INDUSTRY NANJING UNIVERSITY OF TECHNOLOGY
Jiangsu Daocheng Biological Science & Technology Co Ltd
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Priority to CN201610527790.8A priority Critical patent/CN106069137A/en
Publication of CN106069137A publication Critical patent/CN106069137A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14Greenhouses
    • A01G9/1407Greenhouses of flexible synthetic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

Abstract

Plant the implantation methods of Herba Anoectochili roxburghii tissue cultured seedling in the invention discloses a kind of booth, comprise the following steps: the selection in (1) place;(2) construction of booth;(3) construction of moving nursery bed;(4) construction of seedlings nursing plate;(5) selection of substrate;(6) process of tissue cultured seedling;(7) Cultivate administration;(8) gather management;This implantation methods plantation survival rate is high, can reach more than 95%;Growth cycle is short, has only to 45 months from planting to gather;Land utilization ratio is high, bad is not limited by soil is good;The present invention is that the later stage seedling exercising plantation of the plant tissue culture of Herba Anoectochili roxburghii provides technical service;Simple to operate, small investment, the suitable large-scale industrial production of low cost.

Description

The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in a kind of booth
Technical field
The method that the present invention relates to Chinese herbal medicine cultivation, is specifically related to the implantation methods of a kind of Herba Anoectochili roxburghii, particularly to one The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in booth.
Background technology
Herba Anoectochili roxburghii be the orchid family (Orchidaceae) Anoectochilus Blume (gold thread Nelumbo) (Anoectochilus Blume) for many years Raw herbaceous plant, this platymiscium has kind more than 40, is distributed in Tropical Asian regional to Oceania, 20 kinds of China, 2 mutation, mainly Be distributed in Fujian, Guangxi, Guangdong etc. and have the limes marginis of subtropical evergreen broad-leaved, cliff, soil property dredge soft sough.Herba Anoectochili roxburghii is Rare Chinese medicine, enjoys good reputations such as " kings of medicine ", has heat-clearing and toxic substances removing, anti-inflammatory analgetic, skin care of promoting the production of body fluid, strengthens the effects such as immunity.Warp Relevant department measures and finds, in Herba Anoectochili roxburghii, the content of aminoacid composition, composition, content and activity of fighting against senium trace element is above Domestic and wild Radix Panacis Quinquefolii and wild ginseng.Centuries is used as conventional medical herbs among the people.Sugary (the polysaccharide 13. of Herba Anoectochili roxburghii main component 326%, oligosaccharide 11. 243%, reducing sugar 9. 73%), taurine, Cardiac glycosides, esters, alkaloid, stay body, multiple amino Acid, trace element and inorganic elements etc..Wherein taurine, polysaccharide composition have nutrition, defying age, regulation human body immunity of organism Effect.Owing to composition containing carbohydrate content (polysaccharide 13. 326%, oligosaccharide 11. 243%, reducing sugar 9. 73%), cattle sulphur Acid, Cardiac glycosides, esters, alkaloid, stay the compositions such as body, therefore Herba Anoectochili roxburghii have control spitting of blood, bronchitis, nephritis, bladder fire, The function of the solidifying difficult disease such as diabetes, hematuria, rheumatic arthritis, also has certain effect to anticancer.
Herba Anoectochili roxburghii seed germination rate under field conditions (factors) is extremely low, adds and excavates for a long time, and Wild ornamental resources is rare, is on the verge of Extinction.Currently with plant tissue culture technique can substantial amounts of production Herba Anoectochili roxburghii seedling, to some extent solve seedling The problem of shortage, but tissue cultured seedling environment proper capacity is poor, and plantation survival rate is the highest, the invention provides a kind of Herba Anoectochili roxburghii group training The greenhouse gardening of Seedling and management method, not only survival rate is high, and the good growth cycle of growth conditions shortens.
Summary of the invention
The technical problem to be solved in the present invention is to provide that a kind of growth cycle is short, survival rate is high, growth conditions is good and suitably Large-scale productionization effectively solves that Herba Anoectochili roxburghii germ plasm resource is short and plantation Herba Anoectochili roxburghii in the not enough booth of existing cultivation technique The implantation methods of tissue cultured seedling.
For solving above-mentioned technical problem, the technical solution used in the present invention is: plantation Herba Anoectochili roxburghii tissue cultured seedling in this booth Implantation methods, comprises the following steps:
(1) selection in place: select a sunlight can direct projection and draughty barnyard ground, annual mean temperature is more than 10 DEG C;
(2) construction of booth: described booth uses reinforcing bar to build frame, the length 20 ~ 40m of described booth, width 3 ~ 6m, highly 2.65 ~ 2.95m, the device for raising seedlings being provided with in booth is moving nursery bed, fan and warm and humid monitoring system, and frame depends on from the bottom to top Secondary covering Anti-fogging Film, thermal insulation plastic film and fly net;
(3) construction of moving nursery bed: described moving nursery bed includes that seedbed and support, described seedbed are placed in by two bottom sides pulley On the slideway of horizontal alignment, the slideway of described horizontal alignment is fixed on the ground by upright supports;
(4) construction of seedlings nursing plate: described seedbed is several seedlings nursing plates composition, and the total length of several seedlings nursing plates is less than described The length of slideway, described seedlings nursing plate is moved along the slideway of horizontal alignment by described pulley;
(5) selection of substrate: use bark, peat soil, fertile soil and the substrate of perlite mixing, bark, peat soil, fertile soil It is 1:1:1:1 with perlitic mixed proportion;And interpolation quick lime is uniformly mixed and is placed in seedlings nursing plate, described substrate The degree of depth is 6 ~ 10cm;
(6) process of tissue cultured seedling: choosing tissue cultured seedling height is that 8 ~ 10cm tissue culture bottle is placed in booth one week, to adapt in booth The environment such as temperature, illumination and humidity, then take out clear water from bottle and rinse and remove the agar of attachment, then be immersed in concentration To be 0.1% root-inducing powder with concentration be 0.2% ~ 0.5% carbendazim mixed solution in 2 ~ 10 minutes, plant after draining;
(7) Cultivate administration: planting depth is 3 ~ 8cm, planting density be 300 ~ 500 strains/square metre;I.e. irrigate after having planted Water, and it is aerated regulation according to the temperature in canopy and humidity, during follow-up management, substrate is not dry does not waters;The temperature of plantation is protected Holding at 22 ~ 27 DEG C, humidity is maintained at 60 ~ 80%;Carbendazim can be reused after determining to live successfully and fit in solution pouring with root-inducing powder Use, after 7 ~ 10 days, spray blade face, blade face humidity with the potassium dihydrogen phosphate of dilution 300 ~ 500 times;Every two after one month Execute a biphosphate potassium foliage fertilizer week;
(8) gather management: after four months, seedling to 4 ~ 5 slice leaf, plant height reach to gather during 12 ~ 20cm;Plant when gathering More than one section from substrate distance cuts off harvesting.
Use technique scheme, choose sunlight can direct projection and draughty barnyard ground, it is ensured that the illumination of booth And ventilate, the most not only save resource but also cost-effective;Device for raising seedlings moving nursery bed is arranged to transportable, the most any one Individual seedlings nursing plate can be moved along two horizontal slides by pulley, the most just stays the space being available for human users to realize interplanting mould Formula;Use bark, peat soil, fertile soil and perlitic mixed-matrix can improve the survival rate of Herba Anoectochili roxburghii tissue cultured seedling;At base Adding quick lime in matter on the one hand can be to disinfecting soil, on the other hand, can increase the nutrition of substrate, quick lime and base Matter mixes, and can improve the fertilizer efficiency of substrate, is more beneficial for germination and the growth of Herba Anoectochili roxburghii tissue cultured seedling, improves bud ratio and shortening Growth cycle, wherein, the covering of Anti-fogging Film and thermal insulation plastic film can play the effect of insulation, can allow again and receive in canopy uniformly Scattered light, the beneficially growth of Herba Anoectochili roxburghii;When gathering in the plant more than one section distance in substrate in substrate first Above the cutting off of joint is beneficially bred;To keep clean at any time in canopy, in canopy, surrounding is also sterilized with Calx.
Further improvement of the present invention is, described step (1) hollow place includes the barren salt-soda soil of soil resource or dry Nonirrigated farmland.Achieve the motility of place election such barnyard.
Further improvement of the present invention is, described one multilamellar sunshade of step (2) booth peripheral hardware, two ends arrange two The door that individual position is relative, described door sets gradually from inside to outside as plastic foil door, gauze door, sunshade door;Sunshade is outer by interior To being sequentially provided with outward willow sunshade forest belt and gutter.Such setting both can be to being aerated regulation in booth, simultaneously can Illumination adjusting and conditioning is provided in thinking booth.
Further improvement of the present invention is, the usage amount of the described described quick lime in step (5) is every cubic metre mixed Close and substrate is added 400 ~ 800g quick lime.By experimental results demonstrate, the usage amount of quick lime is at 400 ~ 800g/cubic meter Time, with mix in substrate after that the fertilizer efficiency of substrate can be made to bring up to is the highest.
Further improvement of the present invention is, in described step (6), the temperature in booth is maintained at 24 ~ 32 DEG C;Illumination Intensity 1800 ~ 2500LX, humidity is 40 ~ 80%.The tissue cultured seedling height that will be singled out is that 8 ~ 10cm tissue culture bottle is placed in booth one In week, to adapt to the environment such as temperature, illumination and humidity in booth, now the environmental condition such as the temperature of booth, humidity is the most crucial, both Tissue cultured seedling to be ensured adapts to the environment of booth and survives, the growth of tissue cultured seedling to be ensured.
Further improvement of the present invention is, in described step (6), the temperature in booth is maintained at 26 ~ 28 DEG C;Illumination Intensity 2000 ~ 2200LX;Humidity is 55 ~ 65%.
Compared with prior art, the invention has the beneficial effects as follows: 1) plantation survival rate height, can reach more than 95%;2) growth Cycle is short, has only to 4-5 month from planting to gather;3) land utilization ratio is high, bad is not limited by soil is good;4) present invention is The later stage seedling exercising plantation of the plant tissue culture of Herba Anoectochili roxburghii provides technical service;Simple to operate, small investment, low cost is the biggest Technical scale metaplasia is produced.
Specific implementation method
Embodiment 1: the implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in this booth, comprises the following steps:
(1) selection in place: selection sunlight can direct projection and draughty barnyard ground;Can be the barren salt-soda soil of soil resource or Punja;
(2) construction of booth: using reinforcing bar to build frame, length 26.5m, width 6m, highly 2.65m, the nursery set in canopy fills It is set to moving nursery bed, fan and warm and humid monitoring system, frame covers Anti-fogging Film, thermal insulation plastic film and insect protected the most successively Net;One multilamellar sunshade of booth peripheral hardware, two ends arrange two relative doors in position, and described door sets gradually from inside to outside as moulding Material film door, gauze door, sunshade door;Willow sunshade forest belt and gutter it is sequentially provided with from the inside to the outside outside sunshade;
(3) moving nursery bed is built: including seedbed and support, seedbed is placed on the slideway of horizontal alignment by two bottom sides pulley, The slideway of described horizontal alignment is fixed on the ground by upright supports;
(4) seedlings nursing plate is built: seedbed is several seedlings nursing plates composition, and the total length of several seedlings nursing plates is less than described slideway Length, described seedlings nursing plate is moved along the slideway of horizontal alignment by described pulley;Any one seedlings nursing plate can pass through pulley edge Article two, horizontal slide moves, and stays the space being available for human users to realize nixed planting;
(5) substrate select: use bark, peat soil, fertile soil and perlite mixing substrate, bark, peat soil, fertile soil and Perlitic mixed proportion is 1:1:1:1;And add quick lime and be uniformly mixed and be placed in seedlings nursing plate, described substrate deep Degree is 6cm;The usage amount of described quick lime is to add 400 ~ 800g quick lime in the mixed-matrix of every cubic metre;
(6) process of tissue cultured seedling: choosing tissue cultured seedling height is that 8cm tissue culture bottle is placed in booth one week, to adapt to the temperature in booth The environment such as degree, humidity and illumination, the temperature in booth is maintained at 26 DEG C;Intensity of illumination 2000LX;Humidity is 55%;Then from bottle Son takes out clear water and rinses the agar removing attachment, then by soaking 2min in the solution that also root-inducing powder and carbendazim are configured to, drains Rear plantation;
(7) Cultivate administration: planting depth is 3cm, planting density be 300 strains/square metre.I.e. water permeable after having planted, and note Meaning is ventilated, and during follow-up management, substrate is not dry does not waters.Plantation temperature is maintained at 22 ~ 27 DEG C, and humidity is 60 ~ 80%;Can after determining to live successfully Reuse carbendazim and root-inducing powder with the use of, after 7 days, the potassium dihydrogen phosphate of available dilution 500 times sprays blade face, blade face tide Wet;A foliage fertilizer can be executed every two weeks after one month;
(8) gather management: after four months, seedling to 4-5 sheet leaf, plant height can be gathered when reaching 20cm.Can be when gathering More than one section from substrate distance of plant cuts off harvesting.
Plantation survival rate is up to more than 95%, and growth cycle is short, has only to 4-5 month from planting to gather.
Embodiment 2: the implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in this booth, comprises the following steps:
(1) selection in place: selection sunlight can direct projection and draughty barnyard ground;Can be the barren salt-soda soil of soil resource or Punja;
(2) construction of booth: using reinforcing bar to build frame, length 26.5m, width 6m, highly 2.65m, the nursery set in canopy fills It is set to moving nursery bed, fan and warm and humid monitoring system, frame covers Anti-fogging Film, thermal insulation plastic film and insect protected the most successively Net;One multilamellar sunshade of booth peripheral hardware, two ends arrange two relative doors in position, and described door sets gradually from inside to outside as moulding Material film door, gauze door, sunshade door;Willow sunshade forest belt and gutter it is sequentially provided with from the inside to the outside outside sunshade;
(3) moving nursery bed is built: including seedbed and support, seedbed is placed on the slideway of horizontal alignment by two bottom sides pulley, The slideway of described horizontal alignment is fixed on the ground by upright supports;
(4) seedlings nursing plate is built: seedbed is several seedlings nursing plates composition, and the total length of several seedlings nursing plates is less than described slideway Length, described seedlings nursing plate is moved along the slideway of horizontal alignment by described pulley;Any one seedlings nursing plate can pass through pulley edge Article two, horizontal slide moves, and stays the space being available for human users to realize nixed planting;
(5) substrate select: use bark, peat soil, fertile soil and perlite mixing substrate, bark, peat soil, fertile soil and Perlitic mixed proportion is 1:1:1:1;And add quick lime and be uniformly mixed and be placed in seedlings nursing plate, described substrate deep Degree is 6cm;The usage amount of described quick lime is to add 400 ~ 800g quick lime in the mixed-matrix of every cubic metre;
(6) process of tissue cultured seedling: choosing tissue cultured seedling height is that 8cm tissue culture bottle is placed in booth one week, to adapt to the temperature in booth The environment such as degree, humidity and illumination, the temperature in booth is maintained at 28 DEG C;Intensity of illumination 2200LX;Humidity is 65%;Then from bottle Son takes out clear water and rinses the agar removing attachment, then by soaking 2min in the solution that also root-inducing powder and carbendazim are configured to, drains Rear plantation;
(7) Cultivate administration: planting depth is 3cm, planting density be 300 strains/square metre.I.e. water permeable after having planted, and note Meaning is ventilated, and during follow-up management, substrate is not dry does not waters;Plantation temperature is maintained at 22 ~ 27 DEG C, and humidity is 60 ~ 80%;Can after determining to live successfully Reuse carbendazim and root-inducing powder with the use of, after 7 days, the composite foliage fertilizer of available dilution 300 times sprays blade face, blade face Moist;This foliage fertilizer can be executed once every two weeks after one month;
(8) gather management: after four months, seedling to 4-5 sheet leaf, plant height can be gathered when reaching 20cm.Can be when gathering More than one section from substrate distance of plant cuts off harvesting.
Plantation survival rate is up to more than 95%, and growth cycle is short, has only to 4-5 month from planting to gather.
Finally, in addition it is also necessary to be only several specific embodiments of the present invention it is noted that listed above.Obviously, this Bright it is not limited to above example, it is also possible to have many deformation.Those of ordinary skill in the art can be from present disclosure The all deformation directly derived or associate, are all considered as protection scope of the present invention.

Claims (6)

1. the implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in a booth, it is characterised in that comprise the following steps:
(1) selection in place: selecting a barnyard ground, annual mean temperature is more than 10 DEG C;
(2) construction of booth: described booth uses reinforcing bar to build frame, the length 20 ~ 40m of described booth, width 3 ~ 6m, highly 2.65 ~ 2.95m, the device for raising seedlings set in booth is as moving nursery bed, fan and warm and humid monitoring system, on frame the most successively Cover Anti-fogging Film, thermal insulation plastic film and fly net;
(3) construction of moving nursery bed: described moving nursery bed includes that seedbed and support, described seedbed are placed in by two bottom sides pulley On the slideway of horizontal alignment, the slideway of described horizontal alignment is fixed on the ground by upright supports;
(4) construction of seedlings nursing plate: described seedbed is several seedlings nursing plates composition, and the total length of several seedlings nursing plates is less than institute Stating the length of slideway, described seedlings nursing plate is moved along the slideway of horizontal alignment by described pulley;
(5) selection of substrate: use bark, peat soil, fertile soil and the substrate of perlite mixing, bark, peat soil, fertile soil It is 1:1:1:1 with perlitic mixed proportion;And interpolation quick lime is uniformly mixed and is placed in seedlings nursing plate, described substrate The degree of depth is 6 ~ 10cm;
(6) process of tissue cultured seedling: choosing tissue cultured seedling height is that 8 ~ 10cm tissue culture bottle is placed in booth one week, then takes from bottle Go out clear water and rinse the agar removing attachment, then be immersed in many bacterium that concentration is 0.1% root-inducing powder and concentration is 0.2% ~ 0.5% Spirit mixed solution in 2 ~ 10 minutes, after draining plant;
(7) Cultivate administration: planting depth is 3 ~ 8cm, planting density be 300 ~ 500 strains/square metre;I.e. irrigate after having planted Water, and it is aerated regulation according to the temperature in canopy and humidity, during follow-up management, substrate is not dry does not waters;The temperature of plantation is protected Holding at 22 ~ 27 DEG C, humidity is maintained at 60 ~ 80%;Carbendazim can be reused after determining to live successfully and fit in solution pouring with root-inducing powder Use, after 7 ~ 10 days, spray blade face, blade face humidity with the potassium dihydrogen phosphate of dilution 300 ~ 500 times;Every two after one month Execute a biphosphate potassium foliage fertilizer week;
(8) gather management: after four months, seedling to 4 ~ 5 slice leaf, plant height reach to gather during 12 ~ 20cm;Plant when gathering More than one section from substrate distance cuts off harvesting.
The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in booth the most according to claim 1, it is characterised in that described step (1) hollow place includes salt-soda soil or the punja that soil resource is barren.
The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in booth the most according to claim 1, it is characterised in that described step Suddenly one multilamellar sunshade of (2) booth peripheral hardware, two ends arrange two relative doors in position, described door set gradually from inside to outside into Plastic foil door, gauze door, sunshade door;Willow sunshade forest belt and gutter it is sequentially provided with from the inside to the outside outside sunshade.
The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in booth the most according to claim 1, it is characterised in that described step The mixed-matrix that usage amount is every cubic metre of the described quick lime in (5) adds 400 ~ 800g quick lime suddenly.
The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in booth the most according to claim 1, it is characterised in that described step Suddenly in (6), the temperature in booth is maintained at 24 ~ 32 DEG C;Intensity of illumination 1800 ~ 2500LX, humidity is 40 ~ 80%.
The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in booth the most according to claim 5, it is characterised in that described step Suddenly in (6), the temperature in booth is maintained at 26 ~ 28 DEG C;Intensity of illumination 2000 ~ 2200LX;Humidity is 55 ~ 65%.
CN201610527790.8A 2016-07-07 2016-07-07 The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in a kind of booth Pending CN106069137A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106718723A (en) * 2016-11-24 2017-05-31 宣威市绿隆农业有限公司 A kind of nuisanceless high-yield planting method of roxburgh anoectochilus terminal bud
CN107211696A (en) * 2017-06-21 2017-09-29 柳城新天地生态农业发展有限公司 The implantation methods of roxburgh anoectochilus terminal bud
CN107333597A (en) * 2017-06-27 2017-11-10 福建百卉花艺有限公司 A kind of potted plant camellia pest control method
CN107637391A (en) * 2017-11-13 2018-01-30 云南菌视界生物科技有限公司 A kind of red soil area hickory chick cultivation management method
CN107691207A (en) * 2017-11-23 2018-02-16 鹿寨县众生园中药材种植专业合作社 The implantation methods of roxburgh anoectochilus terminal bud
CN110089407A (en) * 2019-04-04 2019-08-06 福建省农业科学院作物研究所 A kind of bagging plantation store method of bud germ plasm resource
CN112425498A (en) * 2020-11-12 2021-03-02 罗定市百草源种养专业合作社 Facility three-dimensional seedling hardening planting method for anoectochilus formosanus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1647610A (en) * 2004-01-19 2005-08-03 福建农林大学 Roxburgh anoectochilus cultivating device and technology
CN102823412A (en) * 2012-09-17 2012-12-19 地缘(厦门)生物科技有限公司 Anoectochilus roburghii pollution-free planting method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1647610A (en) * 2004-01-19 2005-08-03 福建农林大学 Roxburgh anoectochilus cultivating device and technology
CN102823412A (en) * 2012-09-17 2012-12-19 地缘(厦门)生物科技有限公司 Anoectochilus roburghii pollution-free planting method

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106718723A (en) * 2016-11-24 2017-05-31 宣威市绿隆农业有限公司 A kind of nuisanceless high-yield planting method of roxburgh anoectochilus terminal bud
CN106718723B (en) * 2016-11-24 2020-04-10 宣威市绿隆农业有限公司 Pollution-free high-yield planting method for anoectochilus formosanus
CN107211696A (en) * 2017-06-21 2017-09-29 柳城新天地生态农业发展有限公司 The implantation methods of roxburgh anoectochilus terminal bud
CN107333597A (en) * 2017-06-27 2017-11-10 福建百卉花艺有限公司 A kind of potted plant camellia pest control method
CN107637391A (en) * 2017-11-13 2018-01-30 云南菌视界生物科技有限公司 A kind of red soil area hickory chick cultivation management method
CN107691207A (en) * 2017-11-23 2018-02-16 鹿寨县众生园中药材种植专业合作社 The implantation methods of roxburgh anoectochilus terminal bud
CN110089407A (en) * 2019-04-04 2019-08-06 福建省农业科学院作物研究所 A kind of bagging plantation store method of bud germ plasm resource
CN110089407B (en) * 2019-04-04 2021-09-07 福建省农业科学院作物研究所 Bagging planting and storing method for anoectochilus roxburghii germplasm resources
CN112425498A (en) * 2020-11-12 2021-03-02 罗定市百草源种养专业合作社 Facility three-dimensional seedling hardening planting method for anoectochilus formosanus

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Application publication date: 20161109