CN105325097A - Method for promoting seed germination of lithocarpus calophyllus - Google Patents
Method for promoting seed germination of lithocarpus calophyllus Download PDFInfo
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- CN105325097A CN105325097A CN201510739525.1A CN201510739525A CN105325097A CN 105325097 A CN105325097 A CN 105325097A CN 201510739525 A CN201510739525 A CN 201510739525A CN 105325097 A CN105325097 A CN 105325097A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a method for promoting seed germination of lithocarpus calophyllus. The method is characterized by including the procedures of seed collection, seed preprocessing, sand storage germination acceleration and breeding management and protection, wherein by collecting mature lithocarpus calophyllus seeds, sand storage germination acceleration is conducted after seed selection, sterilization, seed soaking and cytokinin processing, and sprouted seeds are obtained through breeding management and protection. According to the method for promoting seed germination of lithocarpus calophyllus, seed dormancy can be effectively broken through only by conducting soaking through plant hormones with a proper concentration and sand storage, seed germination is accelerated, the germination rate is increased, the technological operation is easy, cost is low, operation is convenient, and the method is suitable for popularization and has good economic benefits, social benefits and ecological benefits.
Description
Technical field
The present invention relates to the germination accelerating method of seed, specifically a kind of method promoting Mei Yeke seed germination.
Background technology
Mei Yeke (
lithocarpuscalophyllus) have another name called red autumnal leaves rafter (Jiangxi), sclerophyll C.-glauca, yellow C.-glauca, yellow back of the body oak (Guangdong), person bore (Guangxi), it is Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) arbor, arbor, up to 28 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 1 meter, the epimere of innovation is by the thin micro-pubescence early come off, and biennial branch brown-black, has hole skin.Leaf hard leather matter, wide ellipse, avette or oblong, long 8-15 centimetre, wide 4-9 centimetre, top is point or short prominent point gradually, shape of tail, base portion is bordering on circle or shallow ear-lobe shape, sometimes slightly short the or deflection in side, lateral vein every limit 7-11 bar, cave on blade face, anxious upwards curved and disappear near leaf margin, offshoot is very very thin, is bordering on parallel each other, blade back is without hair, but there is very thick brown color to bronzing, very short panniform, the not plump layer of Powdered squama that fall can be smeared, the blade back canescence of biennial leaf or be slightly with grey grey, wax squama layer consolidation; The long 2.5-5 centimetre of petiole, Inflorescence is identical with star Mao Ke's; Acorn-cup is thick wooden, high 5-10 millimeter, wide 15-25 millimeter; Nut height 15-20 millimeter, wide 18-26 millimeter, top flat central authorities' micro-pits or very short point, often have thin canescence pollen, areola bore 10-14 millimeter, dark 2-4 millimeter.The florescence 6-7 month, the fruit next year 8-9 month is ripe.The dun black of bark, does not ftracture or shallow lobe, thick about 12 millimeters of the bark that the height of tree is about 15 meters, endothelium reddish tan, and ridge rib is very outstanding, up to 3 millimeters, and timber ficelle, quite hard heavy, not very corrosion resistant.Fruit is starch-containing.Produce Jiangxi, the Er Sheng west and south, Fujian, Southern Hunan, Guangdong, Guangxi, South of Guizhou.Be born in height above sea level 500-1200 rice evergreen broad-leaved forest in Taishan mountain.
At present, Mei Yeke resource is still in wild distribution, but along with the multifarious demand of protective plant, large-scale artificial cultivation Mei Yeke resource is imperative.Because Mei Yeke seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And often only have about 40% without the Mei Yeke percentage of seedgermination of any hormone agents process in traditional mode of production, and germinating time evening, emerge irregular, limit the large-scale planting of Mei Yeke.
Summary of the invention
Object of the present invention is the deficiency overcoming existing Mei Yeke Seed germination techniques, provides a kind of germination rate and the quickening germination physiology that can improve Mei Yeke seed, workable, practical simplicity, the method for the promotion Mei Yeke seed germination that cost is low.
Technical scheme of the present invention is as follows:
A kind of method promoting Mei Yeke seed germination, comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe Mei Yeke seed, carry out choosing seeds, sterilize, soak seed and implement after the basic element of cell division process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe Mei Yeke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the Mei Yeke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean Mei Yeke seed being put into volumetric concentration 0.3% soaks 40 ~ 50min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the Mei Yeke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir Mei Yeke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Mei Yeke seed is pulled out and drain;
4. basic element of cell division process: the cell division cellulose solution Mei Yeke seed after seed soaking being put into 100 ~ 300mg/L soaks 3 ~ 4d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, Mei Yeke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
Preferably, the Mei Yeke seed in above step (3) and sand are share equally after the ratio of 1:5 is puddled by volume.
Preferably, the even stand thickness described in above step (3) is no more than 10cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
Preferably, the periodically sprinkle bactericide described in above step (4) be with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8 ~ 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
The present invention relative to prior art, the advantage had and good effect as follows:
1, the method for promotion Mei Yeke seed germination of the present invention, do not need to divide the not easy-operating modes such as cotyledon through mechanical damage removal base portion seed coat, removal unit, only need soak and Sand culture with the cell division cellulose solution of suitable concentration, just effectively seed dormancy can be broken, accelerate seed germination and improve germination rate, sprout nearly 15 days than in traditional mode of production in advance without the Mei Yeke seed of any hormone agents process, germination rate improves more than 40%.
2, the method for promotion Mei Yeke seed germination of the present invention, Mei Yeke seed is through basic element of cell division process and bury vernalization with fine sand, and sprout and shorten perdurabgility, germination peak period Relatively centralized, bud seedling high-quality, whole structure is good.
3, the present invention is placed in nursery by being equipped with the husky flowerpot hiding seed, and with covered rearing with plastic film, and moisturizing is carried out in timing watering, allows seed sprout in its natural state, is applicable to producing.
4, the present invention's potassium permanganate carries out disinfection to seed, and with the mode process sand of high-temperature sterilization, effectively prevents Mei Yeke seed to produce and go mouldy, improve the germination rate of Mei Yeke seed
5, the method for promotion Mei Yeke seed germination of the present invention, technological operation is simple, with low cost, handled easily, is applicable to promoting, and has good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Autumn gathers ripe Mei Yeke seed, is placed in airtight container 2 days.By the Mei Yeke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean Mei Yeke seed puts into volumetric concentration 0.3% is soaked 40min, then pulls out, dry in the shade after rinsing well with clear water.Mei Yeke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir Mei Yeke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Mei Yeke seed is pulled out and drain.The cell division cellulose solution that Mei Yeke seed after seed soaking puts into 100mg/L is soaked 4d.
The baking oven first river sand cleaned up being placed in 100 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by Mei Yeke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 10cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 2:
Autumn gathers ripe Mei Yeke seed, is placed in airtight container 2 days.By the Mei Yeke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean Mei Yeke seed puts into volumetric concentration 0.3% is soaked 45min, then pulls out, dry in the shade after rinsing well with clear water.Mei Yeke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir Mei Yeke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Mei Yeke seed is pulled out and drain.The cell division cellulose solution that Mei Yeke seed after seed soaking puts into 200mg/L is soaked 3d.
The baking oven first river sand cleaned up being placed in 120 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by Mei Yeke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 10cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 9d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 3:
Autumn gathers ripe Mei Yeke seed, is placed in airtight container 3 days.By the Mei Yeke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean Mei Yeke seed puts into volumetric concentration 0.3% is soaked 50min, then pulls out, dry in the shade after rinsing well with clear water.Mei Yeke seed clear water after sterilization is soaked 3d, makes its abundant imbibition; In clear water, fully stir Mei Yeke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Mei Yeke seed is pulled out and drain.The cell division cellulose solution that Mei Yeke seed after seed soaking puts into 300mg/L is soaked 3d.
The baking oven first river sand cleaned up being placed in 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by Mei Yeke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 10cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Claims (4)
1. one kind promotes the method for Mei Yeke seed germination, it is characterized in that: comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe Mei Yeke seed, carry out choosing seeds, sterilize, soak seed and implement after the basic element of cell division process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe Mei Yeke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the Mei Yeke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean Mei Yeke seed being put into volumetric concentration 0.3% soaks 40 ~ 50min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the Mei Yeke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir Mei Yeke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Mei Yeke seed is pulled out and drain;
4. basic element of cell division process: the cell division cellulose solution Mei Yeke seed after seed soaking being put into 100 ~ 300mg/L soaks 3 ~ 4d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, Mei Yeke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
2. the method for promotion Mei Yeke seed germination according to claim 1, is characterized in that: the Mei Yeke seed in step (3) and sand are share equally after the ratio of 1:5 is puddled by volume.
3. the method for promotion Mei Yeke seed germination according to claim 1, is characterized in that: the even stand thickness described in step (3) is no more than 10cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
4. the method for promotion Mei Yeke seed germination according to claim 1, is characterized in that: the periodically sprinkle bactericide described in step (4) be with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8 ~ 10d sprinkling once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
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| CN201510739525.1A CN105325097A (en) | 2015-11-04 | 2015-11-04 | Method for promoting seed germination of lithocarpus calophyllus |
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| CN201510739525.1A CN105325097A (en) | 2015-11-04 | 2015-11-04 | Method for promoting seed germination of lithocarpus calophyllus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613624A (en) * | 2016-10-17 | 2017-05-10 | 柳州三农科技有限公司 | Acquisition method of lithocarpus calophyllus tissue culture explant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103988607A (en) * | 2014-05-23 | 2014-08-20 | 广西壮族自治区林业科学研究院 | Method for promoting germination of lithocarpus polystachyus rehd seeds |
| CN104081980A (en) * | 2014-07-03 | 2014-10-08 | 广西壮族自治区林业科学研究院 | Method for lithocarpus polysachyus rehd container seedling culturing |
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- 2015-11-04 CN CN201510739525.1A patent/CN105325097A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103988607A (en) * | 2014-05-23 | 2014-08-20 | 广西壮族自治区林业科学研究院 | Method for promoting germination of lithocarpus polystachyus rehd seeds |
| CN104081980A (en) * | 2014-07-03 | 2014-10-08 | 广西壮族自治区林业科学研究院 | Method for lithocarpus polysachyus rehd container seedling culturing |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613624A (en) * | 2016-10-17 | 2017-05-10 | 柳州三农科技有限公司 | Acquisition method of lithocarpus calophyllus tissue culture explant |
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