CN106613624A - Acquisition method of lithocarpus calophyllus tissue culture explant - Google Patents
Acquisition method of lithocarpus calophyllus tissue culture explant Download PDFInfo
- Publication number
- CN106613624A CN106613624A CN201610903292.9A CN201610903292A CN106613624A CN 106613624 A CN106613624 A CN 106613624A CN 201610903292 A CN201610903292 A CN 201610903292A CN 106613624 A CN106613624 A CN 106613624A
- Authority
- CN
- China
- Prior art keywords
- yeke
- mei
- explant
- days
- transplanted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an acquisition method of a lithocarpus calophyllus tissue culture explant. The method includes the procedures of seed selection and sowing, bud seedling transplanting and management, stock plant planting and management and explant acquisition. A stock plant is planted after two rounds of selection of excellent seed sources and excellent strong seedlings, and a proper bud strip is cut from the stock pant for disinfection and inoculation. Stock plant management and bud strip acquisition are both very convenient, the obtained explant is excellent in quality, low in contamination rate and death rate, high in adventive bud germination rate and high in operability, buds are orderly, the requirements of establishment of a lithocarpus calophyllus tissue culture rapid propagation system are met, and high economic benefits, social benefits and ecological benefits are achieved.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, is related to a kind of Mei Yeke tissue cultures explant acquisition methods.
Background technology
Mei Yeke（Lithocarpus calophyllus）Also known as red autumnal leaves rafter（Jiangxi）, sclerophyll C.-glauca, Huang C.-glauca, yellow back of the body oak（Extensively
East）, person cone（Guangxi）, it is Fagaceae（Fagaceae）Lithocarpus（Lithocarpus）Arbor, arbor, up to 28 meters, 1 meter of the diameter of a cross-section of a tree trunk 1.3 meters above the ground,
Thin micro- pubescence that the epimere of innovation is early come off, biennial branch brown-black, there is hole skin.Leaf hard leather matter, wide ellipse, it is avette or
Oblong, long 8-15 centimetre, wide 4-9 centimetre, top is tapering or short prominent point, and shape of tail, base portion is bordering on circle or shallow ear-lobe shape, sometimes
Side is slightly short or deflection, and lateral vein, in blade face depression, is suddenly bent up near leaf margin and disappears per side 7-11 bars, and offshoot is very very thin,
It is bordering on each other parallel, blade back has very thick brown color to bronzing, can smear the very short panniform, the powder squama that fall without hair
Not plump layer, the blade back canescence of biennial leaf or slightly with grey grey, wax squama layer consolidation；Petiole is long 2.5-5 centimetre, spends and inflorescence and star
Mao Ke's is identical；Acorn-cup thickness is wooden, high 5-10 millimeters, wide 15-25 millimeters；The high 15-20 millimeters of nut, wide 18-26 millimeters, top
Planar central micro-pits or very short point, often there is thin canescence pollen, areola bore 10-14 millimeters, deep 2-4 millimeters.Florescence
The 6-7 months, the fruit next year 8-9 months are ripe.The dun black of bark, does not ftracture or shallow lobe, and the bark that about 15 meters of the height of tree is thick about 12 millimeters,
Endothelium reddish tan, ridge rib is very projected, up to 3 millimeters, timber ficelle, quite hard weight, not very corrosion resistant.Fruit is starch-containing.Produce
Jiangxi, the Fujian Er Sheng west and south, Southern Hunan, Guangdong, Guangxi, South of Guizhou.It is born in 200 meters of mountain regions of height above sea level 500-1 evergreen wealthy
Ye Linzhong.
At present, Mei Yeke resources are still in wild distribution, but with the demand of protection Plant Diversity, scale people
Work cultivation Mei Yeke resources are imperative.Although at present Mei Yeke is successfully using group culturation rapid propagating technology to the excellent family that selects
System has carried out nursery and has afforested, but in tissue culture procedures, explant material is usually used big tree fringe bar and base portion rudiment
Deng.But big tree majority is located at remote forest zone, plucks inconvenience, rudiment is difficult, quantity is few and is difficult to sterilize, therefore tree coppice shoot greatly is difficult to
Meet actual production nursery demand.And In vitro Embryo, aseptic seedling, cotyledon and needle etc. be used as explant material, it is indefinite to obtain
The speed and quantity of bud is fast and many not as good as stem section, it is difficult to become the common used material of tissue cultures.
The content of the invention
The present invention is in order to overcome the deficiencies in the prior art, there is provided one kind plucks convenient, and quality is good, and quantity is more, and sterilizing is easy, can
Meet the Mei Yeke tissue cultures explant acquisition methods of Mei Yeke tissue-culturing quick-propagation Establishing needs.
In order to realize foregoing invention purpose, technical scheme is as follows：
A kind of Mei Yeke tissue cultures explant acquisition methods, main operational steps are as follows：
（1）Seed selection and sowing：Choose no disease and pests harm, the Mei Yeke superior families seed of full grains is sowed Jing after vernalization process；
（2）Sprout is transplanted and management：Sprout is transplanted to specification in 15cm × 15cm non-woven fabrics Seedling bags, optionally to drench after being unearthed
Water, spray, fertilising；
（3）Maternal plant is planted and management：Work as step（2）Transplanting nursery stock is taken root after stablizing, and is transplanted to specification for 30cm × 30cm containers
In cup, cool canopy is built, selecting strong sprout and being transplanted in the container cup equipped with matrix as maternal plant carries out maternal plant plantation, regularly topdresses, plus
The strong prevention and control of plant diseases, pest control；The component and weight ratio of described matrix is：Yellow soil: coconut palm chaff of becoming thoroughly decomposed: vermiculite: chicken manure is 5: 1: 2: 2；
（4）Explant is obtained：Clip rudiment bar, prunes needle, and carry out disinfection inoculation.
Above-described type of seeding is referred to and for the seed Jing after routine disinfection and hot-water soak to be placed in 100～140 DEG C
Dry in baking oven, naturally cool to and seed is seeded in into what is crossed using the disinfecting solution of potassium permanganate of volumetric concentration 0.3% after room temperature
In sand bed.
Above-described sprout transplanting and management method are transplanted in non-woven fabrics Seedling bag as high 6～8 cm of sprout,
The composite fertilizer's solution of mass concentration 0.5% was applied every 7～10 days, sprout is taken root stable after 40 days.
Above step（3）The cm of height of seedling 40～60 is chosen, the cm of ground diameter 0.3～0.6, trunk is logical straight, the few container seedling of side shoot
As maternal plant.
Above-described maternal plant management method is that the shade of high 1.8～2.5 m is built with the shade net that shade density is 40～60%
Canopy, a volumetric concentration of applying fertilizer every 7～15 days is 0.5～1% composite fertilizer's solution, dense to the inferior quality of foliage-spray one per 20 days
Spend the potassium dihydrogen phosphate for 0.2%～0.5%；After maternal plant is planted 30 days, in blocking trunk at the cm of ground 30.
Above-described explant acquisition methods are clip 6～9cm of length, and the rudiment bar of the cm of diameter 0.2～0.4 uses volume
The alcoholic solution of concentration 75% is carried out after spraying disinfection to rudiment bar, and at 0.5～1.0 cm of rudiment bar top terminal bud is wiped out, remaining
Lower part is from top to bottom trimmed to the stem section of 3.0～4.0 cm length as explant, and every rudiment bar takes 1～2 stem section.
Active ingredient >=48% of the NPK in above-described composite fertilizer's solution.
In contrast to prior art, the present invention has the advantage that and has the beneficial effect that：
1st, the present invention is that maternal plant plantation is carried out in nursery, facilitates maternal plant management and coppice shoot collection；Pass through when maternal plant selects
The two-wheeled of fine provenance and excellent strong sprout is selected, and can well ensure that the fine quality of acquired explant, and in pipe
Under reason, coppice shoot can constantly be sprouted, and growth is fast, can meet the needs of large-scale production.
2nd, the present invention carries out in time cutting strunk to maternal plant and promotees to sprout, and both ensure that enough sprout growth spaces, and preferably
Maintain the equilibrium of nutrition；Suitable rudiment bar is chosen as explant material, its degree of lignification and physiological ageing degree can
Preferably meet tissue culture needs.
4th, the maternal plant matrix that the present invention is prepared according to the physiological property of Mei Yeke, material is easy to get, and nutrition is balanced, reduces life
Cost is produced, the survival rate of maternal plant is improve.
5th, the present invention wipes out terminal bud after sterilization rudiment bar in its top, can remove the young tender terminal bud injured by thimerosal,
Ensure that the postvaccinal survival rate of explant；Every coppice shoot 1-2 stem section of clip from top to bottom, it is ensured that take stem section be respectively provided with it is stronger
Power of regeneration, it is ensured that adventitious bud is concentrated and sprouted during adventitious bud inducing, and rudiment is neat.
6th, maternal plant of the invention management and coppice shoot collection is quite convenient to, and the explant for being obtained is best in quality, pollution rate,
The death rate is low, and adventitious bud germination rate is high, and rudiment is neat, workable, meets Mei Yeke tissue-culturing quick-propagation systems
The demand of foundation, with stronger economic benefit, social benefit and ecological benefits.
Specific embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1：
No disease and pests harm, the Mei Yeke superior families seeds of full grains are chosen, puts in seed Jing after routine disinfection and hot-water soak
Dry in 100～140 DEG C of baking oven, naturally cool to and seed is seeded in into the permanganic acid using volumetric concentration 0.3% after room temperature
In the sand bed that potassium solution was sterilized.
After sprout is unearthed, it is in 15cm × 15cm non-woven fabrics Seedling bags, depending on feelings that specification is transplanted to as high 6～8 cm of sprout
Condition water drenching, spray, and the composite fertilizer's solution of mass concentration 0.5% was applied every 7～10 days, the wherein NPK's in composite fertilizer's solution
Active ingredient >=48%, sprout is taken root stable after 40 days.
After transplanting nursery stock is taken root to be stablized, the cm of height of seedling 40～60 is chosen, the cm of ground diameter 0.3～0.6, trunk is logical straight, side shoot
Few container seedling is transplanted to specification for 30cm × 30cm and is equipped with the container cup of matrix as maternal plant, the group of described matrix
Divide and weight ratio is：Yellow soil: coconut palm chaff of becoming thoroughly decomposed: vermiculite: chicken manure is 5: 1: 2: 2.Built with the shade net that shade density is 40～60%
The cool canopy of high 1.8～2.5 m, every the volumetric concentration of fertilising in 7～15 days be 0.5～1% composite fertilizer's solution, composite fertilizer's solution
In NPK active ingredient >=48%；It is molten to the potassium dihydrogen phosphate that mass concentration of foliage-spray is 0.2～0.5% per 20 days
Liquid；After maternal plant is planted 30 days, in blocking trunk at the cm of ground 30.
Clip 6～9cm of length, the rudiment bar of the cm of diameter 0.2～0.4, with the alcoholic solution of volumetric concentration 75% to rudiment bar
After carrying out spraying disinfection, at 0.5～1.0 cm of rudiment bar top terminal bud is wiped out, remaining part is from top to bottom trimmed to 3.0～
The stem section of 4.0 cm length takes 1～2 stem section as explant, every rudiment bar.
Embodiment 2：
No disease and pests harm, the Mei Yeke superior families seeds of full grains are chosen, puts in seed Jing after routine disinfection and hot-water soak
Dry in 110 DEG C of baking oven, naturally cool to after room temperature seed is seeded in it is molten using the potassium permanganate of volumetric concentration 0.3%
In the sand bed that liquid disinfectant is crossed.
After sprout is unearthed, it is in 15cm × 15cm non-woven fabrics Seedling bags, depending on feelings that specification is transplanted to as high 6～8 cm of sprout
Condition water drenching, spray, and the composite fertilizer's solution of mass concentration 0.5% was applied every 8 days, wherein the NPK's in composite fertilizer's solution is effective
Composition >=48%, sprout is taken root stable after 40 days.
After transplanting nursery stock is taken root to be stablized, the cm of height of seedling 45～50 is chosen, the cm of ground diameter 0.3～0.4, trunk is logical straight, side shoot
Few container seedling is transplanted to specification for 30cm × 30cm and is equipped with the container cup of matrix as maternal plant, the group of described matrix
Divide and weight ratio is：Yellow soil: coconut palm chaff of becoming thoroughly decomposed: vermiculite: chicken manure is 5: 1: 2: 2.Height is built with the shade net that shade density is 50%
The cool canopy of 2.0 m, every the volumetric concentration of fertilising in 8 days be 0.5% composite fertilizer's solution, the NPK in composite fertilizer's solution it is effective into
Divide >=48%；Per 20 days to the potassium dihydrogen phosphate that mass concentration of foliage-spray is 0.2～0.3%；When maternal plant plants 30
After it, in blocking trunk at the cm of ground 30.
Clip 6～8cm of length, the rudiment bar of the cm of diameter 0.2～0.3, with the alcoholic solution of volumetric concentration 75% to rudiment bar
After carrying out spraying disinfection, terminal bud is wiped out at 0.5 cm of rudiment bar top, remaining part is from top to bottom trimmed to the stem of 3.0cm length
Duan Zuowei explants, every rudiment bar takes 1～2 stem section.
Embodiment 3：
No disease and pests harm, the Mei Yeke superior families seeds of full grains are chosen, puts in seed Jing after routine disinfection and hot-water soak
Dry in 120 DEG C of baking oven, naturally cool to after room temperature seed is seeded in it is molten using the potassium permanganate of volumetric concentration 0.3%
In the sand bed that liquid disinfectant is crossed.
After sprout is unearthed, it is in 15cm × 15cm non-woven fabrics Seedling bags, depending on feelings that specification is transplanted to as high 6～8 cm of sprout
Condition water drenching, spray, and the composite fertilizer's solution of mass concentration 0.5% was applied every 9 days, wherein the NPK's in composite fertilizer's solution is effective
Composition >=48%, sprout is taken root stable after 40 days.
After transplanting nursery stock is taken root to be stablized, the cm of height of seedling 50～55 is chosen, the cm of ground diameter 0.4～0.5, trunk is logical straight, side shoot
Few container seedling is transplanted to specification for 30cm × 30cm and is equipped with the container cup of matrix as maternal plant, the group of described matrix
Divide and weight ratio is：Yellow soil: coconut palm chaff of becoming thoroughly decomposed: vermiculite: chicken manure is 5: 1: 2: 2.Built with the shade net that shade density is 40～60%
The cool canopy of high 2.2 m, is 1% composite fertilizer's solution every the volumetric concentration of fertilising in 10 days, and the NPK's in composite fertilizer's solution is effective
Composition >=48%；Per 20 days to the potassium dihydrogen phosphate that mass concentration of foliage-spray is 0.3～0.4%；When maternal plant plantation
After 30 days, in blocking trunk at the cm of ground 30.
Clip 7～8cm of length, the rudiment bar of 0.2～0.3cm of diameter is entered with the alcoholic solution of volumetric concentration 75% to rudiment bar
After row spraying disinfection, terminal bud is wiped out at 0.5～1.0 cm of rudiment bar top, remaining part is from top to bottom trimmed to 3.0～4.0
The stem section of cm length takes 1～2 stem section as explant, every rudiment bar.
Embodiment 4：
No disease and pests harm, the Mei Yeke superior families seeds of full grains are chosen, puts in seed Jing after routine disinfection and hot-water soak
Dry in 140 DEG C of baking oven, naturally cool to after room temperature seed is seeded in it is molten using the potassium permanganate of volumetric concentration 0.3%
In the sand bed that liquid disinfectant is crossed.
After sprout is unearthed, it is in 15cm × 15cm non-woven fabrics Seedling bags, depending on feelings that specification is transplanted to as high 6～8 cm of sprout
Condition water drenching, spray, and the composite fertilizer's solution of mass concentration 0.5% was applied every 10 days, wherein the NPK's in composite fertilizer's solution has
Effect composition >=48%, sprout is taken root stable after 40 days.
After transplanting nursery stock is taken root to be stablized, the cm of height of seedling 55～60 is chosen, the cm of ground diameter 0.5～0.6, trunk is logical straight, side shoot
Few container seedling is transplanted to specification for 30cm × 30cm and is equipped with the container cup of matrix as maternal plant, the group of described matrix
Divide and weight ratio is：Yellow soil: coconut palm chaff of becoming thoroughly decomposed: vermiculite: chicken manure is 5: 1: 2: 2.Height is built with the shade net that shade density is 60%
The cool canopy of 2.5 m, every the volumetric concentration of fertilising in 15 days be 1% composite fertilizer's solution, the NPK in composite fertilizer's solution it is effective into
Divide >=48%；Per 20 days to the potassium dihydrogen phosphate that mass concentration of foliage-spray is 0.4～0.5%；When maternal plant plants 30
After it, in blocking trunk at the cm of ground 30.
Clip 8～9cm of length, the rudiment bar of the cm of diameter 0.3～0.4, with the alcoholic solution of volumetric concentration 75% to rudiment bar
After carrying out spraying disinfection, terminal bud is wiped out at 0.5～1.0 cm of rudiment bar top, remaining part is from top to bottom trimmed to 4.0 cm
Long stem section takes 1～2 stem section as explant, every rudiment bar.
Claims (7)
1. a kind of Mei Yeke tissue cultures explant acquisition methods, it is characterised in that：Main operational steps are as follows：
（1）Seed selection and sowing：Choose no disease and pests harm, the Mei Yeke superior families seed of full grains is sowed Jing after vernalization process；
（2）Sprout is transplanted and management：Sprout is transplanted to specification in 15cm × 15cm non-woven fabrics Seedling bags, optionally to drench after being unearthed
Water, spray, fertilising；
（3）Maternal plant is planted and management：Work as step（2）Transplanting nursery stock is taken root after stablizing, and is transplanted to specification for 30cm × 30cm containers
In cup, cool canopy is built, selecting strong sprout and being transplanted in the container cup equipped with matrix as maternal plant carries out maternal plant plantation, regularly topdresses, plus
The strong prevention and control of plant diseases, pest control；The component and weight ratio of described matrix is：Yellow soil: coconut palm chaff of becoming thoroughly decomposed: vermiculite: chicken manure is 5: 1: 2: 2；
（4）Explant is obtained：Clip rudiment bar, prunes needle, and carry out disinfection inoculation.
2. a kind of Mei Yeke tissue cultures explant acquisition methods according to claim 1, it is characterised in that：Described broadcasts
The method of kind refers to that the seed Jing after routine disinfection and hot-water soak is placed in 100～140 DEG C of baking oven and dries, naturally cools to
Seed is seeded in the sand bed crossed using the disinfecting solution of potassium permanganate of volumetric concentration 0.3% after room temperature.
3. a kind of Mei Yeke tissue cultures explant acquisition methods according to claim 1, it is characterised in that：Described bud
Seedling is transplanted with management method to be transplanted in non-woven fabrics Seedling bag as high 6～8 cm of sprout, and every 7～10 days an inferior quality was applied
The composite fertilizer's solution of concentration 0.5%, sprout is taken root stable after 40 days.
4. a kind of Mei Yeke tissue cultures explant acquisition methods according to claim 1, it is characterised in that：Step（3）
The cm of height of seedling 40～60 is chosen, the cm of ground diameter 0.3～0.6, trunk is logical straight, and the few container seedling of side shoot is used as maternal plant.
5. a kind of Mei Yeke tissue cultures explant acquisition methods according to claim 1, it is characterised in that：Described mother
Strain management method was that the cool canopy of high 1.8～2.5 m is built with the shade net that shade density is 40～60%, every fertilising one in 7～15 days
Secondary volumetric concentration is 0.5～1% composite fertilizer's solution, per 20 days to the phosphoric acid that mass concentration of foliage-spray is 0.2%～0.5%
Dihydro potassium solution；After maternal plant is planted 30 days, in blocking trunk at the cm of ground 30.
6. a kind of Mei Yeke tissue cultures explant acquisition methods according to claim 1, it is characterised in that：Described is outer
Implant acquisition methods are clip 6～9cm of length, the rudiment bar of the cm of diameter 0.2～0.4, with the alcoholic solution pair of volumetric concentration 75%
Rudiment bar is carried out after spraying disinfection, and at 0.5～1.0 cm of rudiment bar top terminal bud is wiped out, and remaining part is from top to bottom trimmed to
The stem section of 3.0～4.0 cm length takes 1～2 stem section as explant, every rudiment bar.
7. a kind of Mei Yeke tissue cultures explant acquisition methods according to claim 3 or 6, it is characterised in that：It is described
Composite fertilizer's solution in NPK active ingredient >=48%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610903292.9A CN106613624A (en) | 2016-10-17 | 2016-10-17 | Acquisition method of lithocarpus calophyllus tissue culture explant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610903292.9A CN106613624A (en) | 2016-10-17 | 2016-10-17 | Acquisition method of lithocarpus calophyllus tissue culture explant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106613624A true CN106613624A (en) | 2017-05-10 |
Family
ID=58856090
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610903292.9A Pending CN106613624A (en) | 2016-10-17 | 2016-10-17 | Acquisition method of lithocarpus calophyllus tissue culture explant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106613624A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220477A (en) * | 2018-10-30 | 2019-01-18 | 华东师范大学 | A kind of paid collection of Ke Youzhi kind and seedling Labor-saving mating system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325097A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting seed germination of lithocarpus calophyllus |
| CN105367271A (en) * | 2015-12-02 | 2016-03-02 | 罗杰 | Specific compound fertilizer for lithocarpus calophyllus and preparation method |
| CN105393839A (en) * | 2015-12-02 | 2016-03-16 | 黄钱英 | Lithocarpuscalophyllus container nursery method |
| CN105393779A (en) * | 2015-12-02 | 2016-03-16 | 黄钱英 | Large scale Lithocarpuscalophyllus seedling cultivation method |
| CN105432294A (en) * | 2015-12-02 | 2016-03-30 | 罗杰 | Lithocarpus calophyllus rapid asexual propagation method |
-
2016
- 2016-10-17 CN CN201610903292.9A patent/CN106613624A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325097A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting seed germination of lithocarpus calophyllus |
| CN105367271A (en) * | 2015-12-02 | 2016-03-02 | 罗杰 | Specific compound fertilizer for lithocarpus calophyllus and preparation method |
| CN105393839A (en) * | 2015-12-02 | 2016-03-16 | 黄钱英 | Lithocarpuscalophyllus container nursery method |
| CN105393779A (en) * | 2015-12-02 | 2016-03-16 | 黄钱英 | Large scale Lithocarpuscalophyllus seedling cultivation method |
| CN105432294A (en) * | 2015-12-02 | 2016-03-30 | 罗杰 | Lithocarpus calophyllus rapid asexual propagation method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220477A (en) * | 2018-10-30 | 2019-01-18 | 华东师范大学 | A kind of paid collection of Ke Youzhi kind and seedling Labor-saving mating system |
| CN109220477B (en) * | 2018-10-30 | 2021-05-07 | 华东师范大学 | Method for collecting high-quality seeds of lithocarpus carpio and breeding seedlings in labor-saving manner |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1922987B (en) | Multiple-ear rock lithocarpus glaber dwarfing rapid reproduction method | |
| CN104081980A (en) | Method for lithocarpus polysachyus rehd container seedling culturing | |
| CN105165512B (en) | Four seasons cuttage and quick-propagation method is preced with Alnus formosana Plantation control partially | |
| CN103828665A (en) | Method for planting se-enriched rice | |
| WO2014190756A1 (en) | Rice ridge-type plant-hole position-setting cultivation method | |
| Narolia et al. | Effect of irrigation schedule and weed-management practices on productivity and profitability of direct-seeded rice (Oryza sativa) in South-eastern Rajasthan | |
| CN103190260A (en) | White bark pine seedling method | |
| CN104106352A (en) | Cultivation method of ligustrun lucidum | |
| CN109566244A (en) | A kind of oil tea implantation methods | |
| CN104584819A (en) | Method for cultivating gold-edge roses on large scale | |
| CN101238792B (en) | Isofemale castor vegetative propagation method | |
| CN105123178A (en) | Plantation method for pseudo-ginseng under trees | |
| CN105453848A (en) | Green high-yield planting technology for mustard | |
| CN104871753A (en) | Red cedrela sinensis seedling growing method | |
| CN103609310B (en) | A kind of alternating method and a kind of tobacco planting method | |
| CN105746115A (en) | Domesticating cultivation method of gymnadenia conopsea | |
| CN103636338B (en) | One grows tobacco fertilizing method and implantation methods | |
| CN107624467A (en) | A kind of fruit of a cubeb litsea tree method for culturing seedlings | |
| CN106258683A (en) | A kind of entire mechanization produces the method for rice rice oil | |
| CN106613624A (en) | Acquisition method of lithocarpus calophyllus tissue culture explant | |
| CN108243812A (en) | The flat Europe hybridization fibert cultivation management method in the north | |
| CN107912202A (en) | A kind of breeding method of Leaf-harvesting Ginkgo seedling | |
| CN106508962B (en) | A kind of seeding growing seedlings method of cypress vernalization composition and its preparation and himalayan cypress | |
| CN102630466A (en) | Method for breeding seed and fresh dual-purpose pumpkin | |
| CN106258457A (en) | Implant acquisition methods outside a kind of Ke Lan Ye tissue culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170510 |
|
| WD01 | Invention patent application deemed withdrawn after publication |