CN105432294A - Lithocarpus calophyllus rapid asexual propagation method - Google Patents
Lithocarpus calophyllus rapid asexual propagation method Download PDFInfo
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- CN105432294A CN105432294A CN201510871451.7A CN201510871451A CN105432294A CN 105432294 A CN105432294 A CN 105432294A CN 201510871451 A CN201510871451 A CN 201510871451A CN 105432294 A CN105432294 A CN 105432294A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a lithocarpus calophyllus rapid asexual propagation method, which comprises working procedures of scion collecting, grafting time and management after grafting, wherein oriental oak container seedlings are selected as rootstocks, healthy and strong semi-lignified and lignified branches without pest and disease damage are clipped from lithocarpus calophyllus seed trees to be used as scions, are grafted by adopting a split grafting, and are managed after grafting, and lithocarpus calophyllus grafted seedlings which are obtained in the spring of the following year can come out a nursery to afforest. The lithocarpus calophyllus rapid asexual propagation method is a breakthrough of lithocarpus calophyllus vegetative propagation technique, utilizes oriental oak rootstocks to graft and multiply, rapidly and largely multiplies the oriental oak container seedlings which keep excellent economic characters of female parents, and is developed in seedling roots. Lithocarpus calophyllus seedlings enable scion woods and rootstocks to rapidly heal and germinate shoots by utilizing the advantages that rootstock oriental oaks are strong in adaptive capacity to natural environment such as drought and infertility and the like, the germinated shoots rapidly grow, and the lithocarpus calophyllus rapid asexual propagation method promotes lithocarpus calophyllus to perfectly, efficiently and rapidly develop, and has excellent economic benefit, social benefit and ecological benefits.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to a kind of Mei Yeke Clonal regeneration method.
Background technology
Mei Yeke (
lithocarpuscalophyllus) have another name called red autumnal leaves rafter (Jiangxi), sclerophyll C.-glauca, yellow C.-glauca, yellow back of the body oak (Guangdong), person bore (Guangxi), it is Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) arbor, arbor, up to 28 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 1 meter, the epimere of innovation is by the thin micro-pubescence early come off, and biennial branch brown-black, has hole skin.Leaf hard leather matter, wide ellipse, avette or oblong, long 8-15 centimetre, wide 4-9 centimetre, top is point or short prominent point gradually, shape of tail, base portion is bordering on circle or shallow ear-lobe shape, sometimes slightly short the or deflection in side, lateral vein every limit 7-11 bar, cave on blade face, anxious upwards curved and disappear near leaf margin, offshoot is very very thin, is bordering on parallel each other, blade back is without hair, but there is very thick brown color to bronzing, very short panniform, the not plump layer of Powdered squama that fall can be smeared, the blade back canescence of biennial leaf or be slightly with grey grey, wax squama layer consolidation; The long 2.5-5 centimetre of petiole, Inflorescence is identical with star Mao Ke's; Acorn-cup is thick wooden, high 5-10 millimeter, wide 15-25 millimeter; Nut height 15-20 millimeter, wide 18-26 millimeter, top flat central authorities' micro-pits or very short point, often have thin canescence pollen, areola bore 10-14 millimeter, dark 2-4 millimeter.The florescence 6-7 month, the fruit next year 8-9 month is ripe.The dun black of bark, does not ftracture or shallow lobe, thick about 12 millimeters of the bark that the height of tree is about 15 meters, endothelium reddish tan, and ridge rib is very outstanding, up to 3 millimeters, and timber ficelle, quite hard heavy, not very corrosion resistant.Fruit is starch-containing.Produce Jiangxi, the Er Sheng west and south, Fujian, Southern Hunan, Guangdong, Guangxi, South of Guizhou.Be born in height above sea level 500-1200 rice evergreen broad-leaved forest in Taishan mountain.
Cork oak (
quercusvariabilisBl) have another name called cork oak, tertia Qinggang, hemp oak, be that Fagaceae (Fagaceae) oak belongs to arbor, tree crown is extensively avette, and trunk is many, taupe, dark lobe, and cork layer spy is thick.Sprig is filbert, and male inflorescence is born in annual shoot bottom, and female flower list is raw or twin with annual shoot axil.May at florescence; The fruit 9-10 month in next year is ripe.Chinese important commerical tree species.Cork oak happiness light, be often born in mountain region tailo, but treelet is there to be lateral shelter as well.To weather, the strong adaptability of soil.The low temperature of ability-20 DEG C, in the acidity of pH4-8, all has growth in neutral and limy soil, also drought-resistant, barren and with deep, fertile, suitably moistening and well-drained loam and sandy loam optimum, not resistance to ponding.Produce the provinces and regions such as Liaoning, Hebei, Shanxi, Shaanxi, Gansu, Shandong, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Henan, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan.North China is born in the tailo of height above sea level less than 800 meters usually, and southwest can reach height above sea level 2000-3000 rice.
At present, Mei Yeke resource is still in wild distribution, but along with keeping the demand of Plant Diversity, large-scale artificial cultivation Mei Yeke resource is imperative.Because Mei Yeke seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And often only have about 50% without the Mei Yeke percentage of seedgermination of any hormone agents process in traditional mode of production, and germinating time evening, emerge irregular, limit the large-scale planting of Mei Yeke.
Summary of the invention
The invention provides a kind of characteristic of cork oak and Mei Yeke can being combined, obtain well developed root system, growth is fast, and survival rate is high, the Mei Yeke Clonal regeneration method of wide adaptability.
To achieve these goals, technical scheme of the present invention is as follows:
A kind of Mei Yeke Clonal regeneration method, comprise the operation of scion collection, grafting time, grafting and managing after grafting, cork oak container seedling carpentery workshop is selected to be stock, from the healthy and strong semi-lignified without damage by disease and insect of Mei Yeke elite stand clip to lignification branch as scion, cleft graft is adopted to carry out grafting, carry out managing after grafting, the Mei Yeke grafting obtained to spring next year can go out garden afforestation; Concrete operation step is as follows:
(1) scion collection: from the healthy and strong semi-lignified without damage by disease and insect of Mei Yeke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel or plastic foil;
(2) grafting time: the four seasons all can carry out grafting;
(3) grafting: the cork oak container seedling nursery stock that leading thread is more than 0.3cm in garden, as stock, adopts cleft graft to carry out grafting;
(4) managing after grafting:
Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, stop fertilising, make the abundant lignification of grafting after entering the winter, the Mei Yeke grafting obtained to spring next year can go out garden afforestation.
Preferably, above-described stock breeding method is as follows:
(1) seedbed and container prepare: select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely;
(2) seed treatment: select common cork oak seed with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1;
(3) sow: germinate February next year to April after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil;
(4) young anvil management: after planting regularly trickle moisturizing, nursery stock strengthens water and fertilizer management and the extermination of disease and insect pest after being unearthed, and cork oak seedling leading thread reaches more than 0.3cm and can be used for grafting.
Preferably, the formula of above-described Nutrition Soil by volume feed is: laterite: humus soil=1 ~ 2: 1; Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 8 ~ 10cm × 15 ~ 20cm.
Preferably, above-described water and fertilizer management and the extermination of disease and insect pest, start fertilising after nursery stock is unearthed 20 days, based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.
Preferably, above-described cleft graft leads to straight place from stock base portion 5 ~ 10cm to cut disconnected, more vertically split down along medulla, dark 1.0 ~ 1.2cm; Being whittled into double inclined plane in the both sides of scion bud is wedge shape, and bevel becomes 30 °, and bevel is long consistent with stock otch, and bevel is level and smooth, retains 1 bud 1 leaf; The scion of cutting is inserted the stock interface of riving, scion and stock form layer alignment, and bud, in outside, is finally used the tighten scion from bottom to top of grafting special plastic film strip, will be strained, prevent scion from moving during colligation.
Relative to prior art, the advantage that the present invention has and good effect as follows:
1, the present invention is the once breakthrough of Mei Yeke vegetative propagation technique, utilizes cork oak stock to carry out propagation by grafiting, and a large amount of Fast-propagation maintains the cork oak container seedling nursery stock of maternal excellent economic characters, and seedling root is flourishing; Mei Yeke wood seedling relies on stock cork oak to the advantageous feature of the stronger adaptive capacity of the natural environment such as non-irrigated, barren, and fringe bar is with stock energy quickly-healing and sprout young sprout, and the young sprout sprouted grows rapid.
2, the present invention selects cork oak as stock, and cultivates with carrying out garden, the good characteristics such as cork oak plant strain growth is rapid, strong, and well developed root system has branch many, and rudiment power is strong, and ground, garden convenient management, simple to operate, survival rate is high.
3, cost of the present invention is low, simple to operate, expands soon numerous, reproduction coefficient is high, and the Mei Yeke nursery stock quantity of acquisition is large, and survival rate is high, and be applicable to downgrading intensive cultivation, promote Mei Yeke high-quality, efficient, fast-developing to there are good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely.Selecting common cork oak seed, with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1.Germinate February next year to March after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil.Nutrition Soil by volume feed is: laterite: humus soil=1 ~ 2: 1 mix obtained.Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 8cm × 15cm.After planting regularly trickle moisturizing, starts fertilising after nursery stock is unearthed 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.Cork oak seedling leading thread reaches more than 0.3cm and can be used for grafting.
Spring or summer, from the healthy and strong semi-lignified without damage by disease and insect of Mei Yeke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel or plastic foil.The cork oak seedling that leading thread is more than 0.3cm in garden, as stock, adopts cleft graft to carry out grafting.Cleft graft leads to straight place from stock base portion 5 ~ 8cm to cut disconnected, more vertically split down along medulla, dark 1.0 ~ 1.2cm; Being whittled into double inclined plane in the both sides of scion bud is wedge shape, and bevel becomes 30 °, and bevel is long consistent with stock otch, and bevel is level and smooth, retains 1 bud 1 leaf; The scion of cutting is inserted the stock interface of riving, scion and stock form layer alignment, and bud, in outside, is finally used the tighten scion from bottom to top of grafting special plastic film strip, will be strained, prevent scion from moving during colligation.Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Nursery stock is unearthed and starts fertilising after 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control, stops fertilising, make the abundant lignification of grafting after entering the winter, and the Mei Yeke grafting obtained to spring next year can go out garden afforestation.
Embodiment 2:
Select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely.Selecting common cork oak seed, with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1.Germinate March next year to April after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil.Nutrition Soil by volume feed is: laterite: humus soil=2: 1 mix obtained.Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 10cm × 20cm.After planting regularly trickle moisturizing, starts fertilising after nursery stock is unearthed 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.Cork oak seedling leading thread reaches more than 0.3cm and can be used for grafting.
Autumn or winter, from the healthy and strong semi-lignified without damage by disease and insect of Mei Yeke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with plastic foil.The cork oak seedling that leading thread is more than 0.3cm in garden, as stock, adopts cleft graft to carry out grafting.Cleft graft leads to straight place from stock base portion 8 ~ 10cm to cut disconnected, more vertically split down along medulla, dark 1.0 ~ 1.2cm; Being whittled into double inclined plane in the both sides of scion bud is wedge shape, and bevel becomes 30 °, and bevel is long consistent with stock otch, and bevel is level and smooth, retains 1 bud 1 leaf; The scion of cutting is inserted the stock interface of riving, scion and stock form layer alignment, and bud, in outside, is finally used the tighten scion from bottom to top of grafting special plastic film strip, will be strained, prevent scion from moving during colligation.Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Nursery stock is unearthed and starts fertilising after 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control, stops fertilising, make the abundant lignification of grafting after entering the winter, and the Mei Yeke grafting obtained to spring next year can go out garden afforestation.
Claims (5)
1. Yi Zhong Mei Yeke Clonal regeneration method, it is characterized in that: the operation comprising scion collection, grafting time, grafting and managing after grafting, cork oak container seedling carpentery workshop is selected to be stock, from the healthy and strong semi-lignified without damage by disease and insect of Mei Yeke elite stand clip to lignification branch as scion, cleft graft is adopted to carry out grafting, carry out managing after grafting, the Mei Yeke grafting obtained to spring next year can go out garden afforestation; Concrete operation step is as follows:
(1) scion collection: from the healthy and strong semi-lignified without damage by disease and insect of Mei Yeke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel or plastic foil;
(2) grafting time: the four seasons all can carry out grafting;
(3) grafting: the cork oak container seedling nursery stock that leading thread is more than 0.3cm in garden, as stock, adopts cleft graft to carry out grafting;
(4) managing after grafting:
Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, stop fertilising, make the abundant lignification of grafting after entering the winter, the Mei Yeke grafting obtained to spring next year can go out garden afforestation.
2. a kind of Mei Yeke Clonal regeneration method according to claim 1, is characterized in that: described stock breeding method is as follows:
(1) seedbed and container prepare: select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely;
(2) seed treatment: select common cork oak seed with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1;
(3) sow: germinate February next year to April after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil;
(4) young anvil management: after planting regularly trickle moisturizing, nursery stock strengthens water and fertilizer management and the extermination of disease and insect pest after being unearthed, and cork oak seedling leading thread reaches more than 0.3cm and can be used for grafting.
3. a kind of Mei Yeke Clonal regeneration method according to claim 2, is characterized in that: the formula of described Nutrition Soil by volume feed is: laterite: humus soil=1 ~ 2: 1; Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 8 ~ 10cm × 15 ~ 20cm.
4. according to the arbitrary a kind of described Mei Yeke Clonal regeneration method of claim 1 or 2, it is characterized in that: described water and fertilizer management and the extermination of disease and insect pest, fertilising is started after nursery stock is unearthed 20 days, based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.
5. a kind of Mei Yeke Clonal regeneration method according to claim 1, is characterized in that: described cleft graft leads to straight place from stock base portion 5 ~ 10cm to cut disconnected, more vertically split down along medulla, dark 1.0 ~ 1.2cm; Being whittled into double inclined plane in the both sides of scion bud is wedge shape, and bevel becomes 30 °, and bevel is long consistent with stock otch, and bevel is level and smooth, retains 1 bud 1 leaf; The scion of cutting is inserted the stock interface of riving, scion and stock form layer alignment, and bud, in outside, is finally used the tighten scion from bottom to top of grafting special plastic film strip, will be strained, prevent scion from moving during colligation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258990A (en) * | 2016-10-17 | 2017-01-04 | 柳州玲通科技有限责任公司 | A kind of Mei Yeke initial culture bud inducement method |
| CN106613624A (en) * | 2016-10-17 | 2017-05-10 | 柳州三农科技有限公司 | Acquisition method of lithocarpus calophyllus tissue culture explant |
| CN108718747A (en) * | 2018-05-24 | 2018-11-02 | 郴州市林业科学研究所 | North America Oak Tree method for culturing seedlings |
| CN112492994A (en) * | 2020-11-26 | 2021-03-16 | 山东省林木种质资源中心 | Method for budding quercus acutissima and quercus variabilis |
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|---|---|---|---|---|
| CN1062260A (en) * | 1990-12-10 | 1992-07-01 | 刘维清 | The method of scion grafting of chestnut branch on oaks (cupule family) |
| CN103975739A (en) * | 2014-05-16 | 2014-08-13 | 广西壮族自治区林业科学研究院 | Propagation method of lithocarpus polysachyus rehd young stock grafting container-grown seedling |
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2015
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1062260A (en) * | 1990-12-10 | 1992-07-01 | 刘维清 | The method of scion grafting of chestnut branch on oaks (cupule family) |
| CN103975739A (en) * | 2014-05-16 | 2014-08-13 | 广西壮族自治区林业科学研究院 | Propagation method of lithocarpus polysachyus rehd young stock grafting container-grown seedling |
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| 王连珍等: "柞树种质资源研究进展", 《蚕业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258990A (en) * | 2016-10-17 | 2017-01-04 | 柳州玲通科技有限责任公司 | A kind of Mei Yeke initial culture bud inducement method |
| CN106613624A (en) * | 2016-10-17 | 2017-05-10 | 柳州三农科技有限公司 | Acquisition method of lithocarpus calophyllus tissue culture explant |
| CN108718747A (en) * | 2018-05-24 | 2018-11-02 | 郴州市林业科学研究所 | North America Oak Tree method for culturing seedlings |
| CN112492994A (en) * | 2020-11-26 | 2021-03-16 | 山东省林木种质资源中心 | Method for budding quercus acutissima and quercus variabilis |
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