CN101238792B - Isofemale castor vegetative propagation method - Google Patents
Isofemale castor vegetative propagation method Download PDFInfo
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- CN101238792B CN101238792B CN200710013492A CN200710013492A CN101238792B CN 101238792 B CN101238792 B CN 101238792B CN 200710013492 A CN200710013492 A CN 200710013492A CN 200710013492 A CN200710013492 A CN 200710013492A CN 101238792 B CN101238792 B CN 101238792B
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- castor
- oil plant
- ricin
- cutting
- cuttage
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Abstract
The invention provides a haplometrotic ricin asexual propagation method belonging to the crop heterosis utilization field. The invention is characterized in that: A. transplant and preserve the wholeplant of single female ricin material which can not be sexually reproduced into the sunlight greenhouse before frost; B. cut hard branches with three leaves when the temperature is up to 20 DEG C in the next spring, dip the lower part of the ricin cutting into 2000 times solution of 20% a-NAA powder in 2 hours; C. root the hard branch of ricin in nursery bed containing fine sand or fine soil, thedepth is 10-15cm; D. cultivate the cutting at 20-28 DEG C, under 70-80% humidity, with the moist substrate and scattered light; E. train the branches 5-10 days before being planted into field, increase the light and ventilation time. The invention not only has the merits of retaining precious ricin single female breeding material, but also breeding lots of single female ricin material many times using branches of single plant.
Description
Technical field
The present invention relates to single pistillate castor vegetative propagation, breeding hybridized castor parent female series seed selection and asexual propagation method belong to agricultural thing heterosis utilization field.
Background technology
Castor-oil plant hybrid vigour is fairly obvious, and crossbreed is than the conventional generally volume increase 20-30% that plants.Advantage combination volume increase is more than 100%.And the key of seed selection breeding hybridized castor is at first to breed the castor-oil plant female series.Castor-oil plant is Euphorbiaceae, the coaxial different flower of male and female flowering homophyletic, and female flower is grown thickly in raceme top, and male flower and is born in the floral axis bottom.The single pistillate castor inflorescence only give birth to female flower, not give birth to male flower.It is very little that probability takes place unisexuality female plant castor-oil plant under field conditions (factors).Have only by breeding material is carried out inbreeding of more generation, or in offspring's variation that the method for Cor60 x ray irradiation x, chemistry, physics is handled just idol discovery is arranged, be of great rarity comparatively speaking.The whole nation tens of families of castor-oil plant breeding units, the pure female system of the castor-oil plant of breeding has only 5.Most breeding units are found behind the single female material of castor-oil plant owing to do not have adequate time and method that its generation male flower pollination self sexual propagation is kept.Wither away when frost arrives thereupon, thereby seriously restricted the seed selection process of castor-oil plant female series.
Summary of the invention
The technical problem to be solved in the present invention is: overcome the problem that prior art exists, a kind of precious single pistillate castor breeding material that not only effectively kept is provided, and the branch that utilizes an individual plant can repeatedly breed countless unisexuality female plant castor-oil plants, thereby for inducing male flower selfed breeding castor-oil plant female series to create condition.Also can be used for being difficult to induce the vegetative propagation of male flower castor-oil plant female series simultaneously, utilize vegetative castor-oil plant female series directly to prepare the single pistillate castor vegetative propagation method of castor-oil plant first-filial generation kind.
The technical solution adopted for the present invention to solve the technical problems is: this single pistillate castor vegetative propagation method is characterized in that:
Castor-oil plant unisexuality female plant is preserved in A, continuity, the single female material of castor-oil plant earlier whole strain before frost that can not realize sexual propagation heredity continuity of finding is transplanted in winter warm type big shack preserve;
B, spring next year, the temperature hard branch of after 20 ℃ three blades of clip band that gos up soaked castor-oil plant cutting bottom 2 hours with 2000 times of liquid of 20%a methyl pulvis;
C, with the castor-oil plant cuttage in the bed of growing seedlings that fine sand or fine earth are housed, water the cuttage of permeable back earlier, degree of depth 10-15cm;
D, cutting is remained on 20-28 ℃, humidity 70-80% cultivates under the moistening astigmatic condition of matrix, and 10-20 days begin to take root, and root system can reach the 10-30 bar in 25-35 days, the newborn branch 1-3 of overground part, can transplant in the land for growing field crops during blade 3-6 sheet;
Pruned in 5-10 days before E, the field planting land for growing field crops, cut off big portion branches and leaves on the ground, only keep 1 newborn twig of stalwartness of base portion, increase ventilation and light application time and carry out hardening.
Preserving the castor-oil plant female series, is that the hard branch of three blades of direct clip band carries out cuttage.
The castor-oil plant cuttage or be loaded on fine sand or the seedling-growing container of fine earth, the plastic sack of growing seedlings in.
Soak the castor-oil plant cutting with the 20%a methyl or do not soak castor-oil plant and transplant a cutting direct cuttage and also can take root with the hard branch of three blades.
Compared with prior art, the beneficial effect that the present invention had is: not only effectively kept precious breeding resource " single pistillate castor material ", but and the branch vegetative propagation that utilizes 1 individual plant goes out countless strain single pistillate castors, thereby for inducing male flower selfing purifying, seed selection castor-oil plant female series to create condition.This technology also can be used for the pierre of castor-oil plant female series simultaneously.
Embodiment
Embodiment 1:
Induce the male flower elite clone of difficulty especially with the single female material C 89-3 of the stingless type castor-oil plant of capsule, the female material C 02-6 of spinosity type list, three of C9041A, transplant in winter warm type big shack October last year, next year beta pruning on April 23, soak castor-oil plant cutting bottom cuttage in 2 hours with 2000 times of liquid of 20%a methyl pulvis, cutting is remained on 20-28 ℃, humidity 70-80% cultivates under the moistening astigmatic condition of matrix, begins to take root after 10-15 days.C9041A was taken root the fastest 10 days.C02-6 was taken root the slowest 15 days.Cuttage 11 of minimum root systems after 30 days, 45 of maximum root systems, the long 25cm of root.May 22 next year, training only kept 1 healthy and strong twig of base portion, and all the other are all cut, and stopped water spray and increased the illumination hardening about 5 days, transplanted in the land for growing field crops, saw the following form.
Cuttage root-taking transplanting survival rate statistical form
| Material number | Cuttage (bar) | Survive (bar) | Survival rate % | On average take root (bar) | Transplant number (bar) | Survive number (bar) | Survival rate % |
| C89-3 | 20 | 20 | 100 | 29 | 20 | 18 | 90 |
| C02-6 | 20 | 17 | 85 | 11 | 17 | 16 | 94 |
| C9041A | 20 | 19 | 95 | 22 | 19 | 17 | 89 |
More than the single female materials of three castor-oil plant transplant respectively in three different isolated areas, induce the male flower success through artificial agronomic measures, obtain the selfed seed of varying number respectively.Late October next year is seeded in Sanya, Hainan with selfed seed.
Embodiment 2:
Find a large spike single pistillate castor material last year, through artificial 8 harvesting female flowers, last 25 days 1 time every harvesting in 3 days, 1 inflorescence is plucked 1003 of female flowers (general castor-oil plant female flower is no more than 300) altogether, does not induce the generation male flower yet.Passing temperature in time descends to inducing and has not had possibility in male flower selfing year.Carry out the hard branch of clip September 2 next year, the hard branch of three blades of clip band is soaked castor-oil plant cutting 2 hours with the 20%a methyl, cuttage is loaded on degree of depth 10-15cm in the bed of growing seedlings of fine sand or fine earth; Cutting is remained on 20-28 ℃, and humidity 70-80% cultivates under the moistening astigmatic condition of matrix, September 25 per day taking root more than 20, September 26 training hardening was transplanted booth October 6 and is survived the winter, and preserved the female excellent germplasm material of this list.Treat to lure again next year to spend to educate and be.
Cuttage or be loaded on seedling-growing container, in the plastic sack of growing seedlings.
Not soaking castor-oil plant transplants a cutting direct cuttage and also can take root.
Utilize this technology not only effectively to keep precious breeding single pistillate castor material, but and utilize the countless strain single pistillate castors of branch vegetative propagation of 1 individual plant.
Thereby for inducing male flower selfed breeding castor-oil plant female series to create condition, this technology also can be used for the pierre of castor-oil plant female series simultaneously.
Claims (3)
1. single pistillate castor vegetative propagation method is characterized in that:
Castor-oil plant unisexuality female plant is preserved in A, continuity, the single female material of castor-oil plant earlier whole strain before frost that can not realize sexual propagation heredity continuity of finding is transplanted in winter warm type big shack preserve;
B, spring next year, the temperature hard branch of after 20 ℃ three blades of clip band that gos up soaked castor-oil plant cutting bottom 2 hours with 2000 times of liquid of 20% αNai Yisuan pulvis;
C, with the castor-oil plant cuttage in the bed of growing seedlings that fine sand or fine earth are housed, water the cuttage of permeable back earlier, degree of depth 10-15cm;
D, cutting is remained on 20-28 ℃, humidity 70-80% cultivates under the moistening astigmatic condition of matrix, and 10-20 days begin to take root, and root system can reach the 10-30 bar in 25-35 days, and the newborn branch 1-3 of overground part, blade 3-6 transplant in the land for growing field crops during sheet;
Pruned in 5-10 days before E, the field planting land for growing field crops, cut off big portion branches and leaves on the ground, only keep 1 newborn twig of stalwartness of base portion, increase ventilation and light application time and carry out hardening.
2. single pistillate castor vegetative propagation method according to claim 1 is characterized in that: the castor-oil plant cuttage be loaded on fine sand or the seedling-growing container of fine earth, the plastic sack of growing seedlings in.
3. single pistillate castor vegetative propagation method according to claim 1 is characterized in that: soak the castor-oil plant cutting with 20% αNai Yisuan or do not soak castor-oil plant and transplant a cutting direct cuttage and also can take root with the hard branch of three blades.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710013492A CN101238792B (en) | 2007-02-06 | 2007-02-06 | Isofemale castor vegetative propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710013492A CN101238792B (en) | 2007-02-06 | 2007-02-06 | Isofemale castor vegetative propagation method |
Publications (2)
| Publication Number | Publication Date |
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| CN101238792A CN101238792A (en) | 2008-08-13 |
| CN101238792B true CN101238792B (en) | 2010-05-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710013492A Expired - Fee Related CN101238792B (en) | 2007-02-06 | 2007-02-06 | Isofemale castor vegetative propagation method |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347093B (en) * | 2008-08-28 | 2012-10-17 | 吉林大学 | Method for quickly cultivating thermosensitive type recessive nueleus sterility isofemale line castor and use |
| CN102090317B (en) * | 2009-12-10 | 2014-03-19 | 黄家祥 | Breeding method and hybrid seeding method for castor unisexual flower inbred lines |
| CN102668860A (en) * | 2012-06-13 | 2012-09-19 | 云南省农业科学院经济作物研究所 | High-altitude press-grafting method for female individual plants of Ricinus communis |
| CN102823409B (en) * | 2012-09-13 | 2013-05-29 | 云南省农业科学院经济作物研究所 | Cutting propagation method of ricinus communis |
| CN102934610A (en) * | 2012-10-23 | 2013-02-20 | 中国农业科学院油料作物研究所 | Breeding maintaining method of ricinus communist female plant |
| CN104542259A (en) * | 2015-01-15 | 2015-04-29 | 山东家祥蓖麻种业科技有限公司 | Breeding method capable of identifying purity of hybrid seedlings in castor seedlings |
| CN107493882A (en) * | 2017-08-29 | 2017-12-22 | 长葛市联发粮食专业合作社 | Castor-oil plant method for culturing seedlings |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1488242A (en) * | 2003-09-09 | 2004-04-14 | 山西绿洲农业科技有限公司 | Method for planting high-yield dwarf-stem single spike castor-oil plant |
| CN1759655A (en) * | 2004-10-14 | 2006-04-19 | 马立民 | Method for grafting and breeding high production castor bean |
-
2007
- 2007-02-06 CN CN200710013492A patent/CN101238792B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1488242A (en) * | 2003-09-09 | 2004-04-14 | 山西绿洲农业科技有限公司 | Method for planting high-yield dwarf-stem single spike castor-oil plant |
| CN1759655A (en) * | 2004-10-14 | 2006-04-19 | 马立民 | Method for grafting and breeding high production castor bean |
Non-Patent Citations (3)
| Title |
|---|
| 李文昌.蓖麻雌性单株两种无性繁殖技术研究及繁殖体系构建.中国农业大学硕士学位论文.2005,24-29. * |
| 黄家祥等.高油蓖麻新品种淄蓖麻2号.中国种业 1.2001,(1),25. |
| 黄家祥等.高油蓖麻新品种淄蓖麻2号.中国种业 1.2001,(1),25. * |
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| CN101238792A (en) | 2008-08-13 |
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